International Journal of Environmental Research and Public Health
·
2013
·Vol. 10
·期 7
·2897-2905
·
IF 1.998
Juan Peng,Shanshan Song,Liguang Xu,Wei Ma,Liqiang Liu,Hua Kuang,Chuanlai Xu
We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.Keywords:peanut allergen;Ara h 1;monoclonal antibody;sandwich ELISA
研究领域:
食品科学
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免疫学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Wenshan He,Run Jiang,Siqi Li,Minglu Zhang,Tingting Zhang,Xiaobiao Zhu,Xiaohui Wang
Chlortetracycline (CTC), a widely used typical tetracycline antibiotic, has raised increasing concerns due to its potential health and environmental risks. Biodegradation is considered an effective method to reduce CTC in environment. In this study, a strain Aspergillus sp. LS-1, which can efficiently degrade CTC, was isolated from CTC-rich activated sludge . Under optimal conditions, the maximum removal efficiency of CTC could reach 95.41%. Temperature was the most significant factor affecting the degradation efficiency of LS-1. The 19 products were identified in the CTC degradation by strain LS-1, and three degradation pathways were proposed. All the degradation pathways for CTC exhibited ring-cleaving, which may accelerate the mineralization of CTC. To gain more comprehensive insights into this strain, we obtained the genome of LS-1, which had high GC content (50.1%) and completeness (99.3%). The gene annotation revealed that LS-1 contains some vital enzymes and resistance genes that may carry functional genes involved in the CTC degradation. In addition, other antibiotic resistance genes were found in the genome of LS-1, indicating that LS-1 has the potential to degrade other antibiotics. This study provides a more theoretical basis for the investigation of CTC degradation by fungi and new insights into the biodegradation of CTC.
研究领域:
环境科学
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微生物学
·
基因组学
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DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
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2024
·
IF 4.8
Ying Zhang,Qinqin Wang,Mingyuan Zhu,Bin Dai
The prevailing Zn/AC catalysts was plagued by limited lifetime and difficulties in regeneration for acetylene acetoxylation. In order to exploring renewable catalyst for acetylene acetoxylation, Zn/S-1 catalyst was synthesized using Silicalite-1(S-1) as support. The catalytic performance of Zn/S-1 was compared with Zn/AC catalyst using active carbon as support. The CH 3 COOH conversion of Zn/S-1 catalyst only decreased 15 % after running 225 h, much better than the 66 % of Zn/AC after 154 h. TG analysis revealed that the improved stability of Zn/S-1 catalyst was attributed to its better resistance for carbon deposition with the presence of S-1 support. A series of characterizations and DFT results elucidated that the Lewis acid sites of S-1 support and Zn component serve as dual active sites, which enhanced the adsorption of CH 3 COOH and inhibited the carbon deposition. In addition, the deactivated Zn/S-1 catalyst could be regenerated by calcination in air atmosphere due to the thermal stability of S-1, and the regenerated Zn/S-1 catalyst showed similar catalytic activity with the fresh catalyst.
研究领域:
催化化学
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材料科学
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产品关联
DOI
高影响因子
CANCER RESEARCH
·
2022
·Vol. 82
·期 19
·3573-3587
·
IF 13.312
Li Yongxu,Wang Tiepeng,Wan Quan,Wang Qing,Chen Zhenzhong,Gao Yuan,Ye Yuchen,Lin Jiusheng,Zhao Bihuan,Wang Huaile,Yang Jinming,Zhao Kai,Lu Na
Glioblastoma (GBM) is the most common type of primary adult brain tumor. Glioma stem cell (GSC) residence and temozolomide (TMZ) resistance in GBM both contribute to poor patient outcome. TRAF4 is a scaffold protein with E3 ubiquitin ligase activity that has recently been discovered to promote invasion and metastasis in several malignancies, but the effects and functions of TRAF4 in GBM remain to be determined. Here, we report that TRAF4 is preferentially overexpressed in GSCs and is required for stem-like properties as well as TMZ sensitivity in GBM cells. TRAF4 specifically interacted with the N-terminal tail of Caveolin-1 (CAV1), an important contributor to the tumorigenicity of GBM cells. TRAF4 regulated CAV1 stability by preventing ZNRF1-mediated ubiquitination and facilitating USP7-mediated deubiquitination independently of its E3 ubiquitin ligase catalytic activity. TRAF4-mediated stabilization of CAV1 activated protumorigenic AKT/ERK1/2 signaling, and disruption of this axis resulted in defects in stemness maintenance. In addition, expression of TRAF4 and CAV1 was positively correlated and predicted poor prognosis in human GBM samples. Screening of common nervous system drugs identified risperidone interaction with TRAF4, and risperidone treatment resulted in the dissociation of TRAF4 and CAV1. Importantly, pharmacologic inhibition of TRAF4 with risperidone potently inhibited self-renewal, abrogated tumorigenicity, and reversed TMZ resistance in GBM. Overall, TRAF4-mediated stabilization of CAV1 promotes stemness and TMZ resistance in GBM, providing a therapeutic strategy that could improve patient outcomes. Significance: The identification of a TRAF4/Caveolin-1 axis that plays a crucial role in malignant progression of glioblastoma provides new insights into the function of TRAF4 in ubiquitin signaling and suggests TRAF4 as a potential therapeutic target.
研究领域:
肿瘤学
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神经科学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE
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2025
·
IF 3.6
Shufang Wu,Zhiqian Long,Yushu Zhang,Ting Zhang,Qinghong Luo,Xiaoxuan Liu,Zhiquan Li
Using low catalyst loadings while maintaining precise polymer property control is a critical challenge in the O-ATRP field. Herein, we report a novel O-ATRP system utilizing 1-bromopyrene (Py-Br) as a photoredox catalyst, enabling controlled polymerization of methyl methacrylate (MMA) at an ultra-low loading of 10 ppm. When combined with ethyl α -bromophenylacetate (EBPA) as an initiator, this system produces polymers with a molecular weight distribution of 1.48 and an initiation efficiency of 66.7%. Mechanistic studies, including UV–Vis spectroscopy, fluorescence spectroscopy, cyclic voltammetry, and time-resolved fluorescence measurements, elucidate the photophysical and photochemical properties of Py-Br in O-ATRP. Kinetic analyses reveal first-order polymerization kinetics, while the linear increase of molecular weight with monomer conversion and low dispersity values indicate good control over the polymerization. The living nature of the system is further confirmed through successful chain extension experiments. This Py-Br-catalyzed O-ATRP system explores the feasibility of conducting polymerization at lower catalyst concentrations, offering insights into approaches for reducing catalyst usage in polymer synthesis. Graphical Photoinduced metal-free ATRP of MMA using ppm-level Py-Br as the organic photocatalyst.
研究领域:
高分子化学
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材料科学
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光催化
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产品关联
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2018
·
IF 4.556
Sida Ruan,Yuanyuan Gu,Bo Liu,Huashan Gao,Xiongwei Hu,Haiping Hao,Liang Jin,Ting Cai
Glucagon-like peptide 1 (GLP-1) has recently received significant attention as an efficacious way to treat diabetes mellitus. However, the short half-life of the peptide limits its clinical application in diabetes. In our previous study, a novel GLP-1 analog (PGLP-1) with a longer half-life was synthesized and evaluated. Herein, we prepared the PGLP-1-loaded poly(d,l-lactide-co-glycolide) microspheres to achieve long-term effects on blood glucose control. The incorporation of zinc ion into the formulation can effectively decrease the initial burst release, and a uniform drug distribution was obtained, in contrast to native PGLP-1 encapsulated microspheres. We demonstrated that the solubility of the drug encapsulated in microspheres played an important role in in vitro release behavior and drug distribution inside the microspheres. The Zn-PGLP-1 microspheres had a prominent acute glucose reduction effect in the healthy mice. A hypoglycemic effect was observed in the streptozotocin (STZ) induced diabetic mice through a 6-week treatment of Zn-PGLP-1-loaded microspheres. Meanwhile, the administration of Zn-PGLP-1 microspheres led to the β-cell protection and stimulation of insulin secretion. The novel GLP-1 analog-loaded sustained microspheres may greatly improve patient compliance along with a desirable safety feature.
研究领域:
药学
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生物医学工程
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内分泌学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL MICROBIOLOGY
·
2024
·
IF 2.3
Pan Lixu,Yuan Bo,Li Qingqing,Ouyang Ji,Yang Jie,Zhou Yan,Cui Changzheng
Chlorobenzene (CB), extensively used in industrial processes, has emerged as a significant contaminant in soil and groundwater. The eco-friendly and cost-effective microbial remediation has been increasingly favored to address this environmental challenge. In this study, a degrading bacterium was isolated from CB-contaminated soil at a pesticide plant, identified as Pandoraea sp. XJJ-1 (CCTCC M 2021057). This strain completely degraded 100 mg·L −1 CB and showed extensive degradability across a range of pH (5.0–9.0), temperature (10–37 °C), and CB concentrations (100–600 mg·L −1 ). Notably, the degradation efficiency was 85.2% at 15 °C, and the strain could also degrade six other aromatic hydrocarbons, including benzene, toluene, ethylbenzene, and xylene ( o- , m- , p- ). The metabolic pathway of CB was inferred using ultraperformance liquid chromatography, gas chromatography-mass spectrometry, and genomic analysis. In strain XJJ-1, CB was metabolized to o -chlorophenol and 3-chloroxychol by CB monooxygenase, followed by ortho- cleavage by the action of 3-chlorocatechol 1,2-dioxygenase. Moreover, the presence of the chlorobenzene monooxygenation pathway metabolism in strain XJJ-1 is reported for the first time in Pandoraea . As a bacterium with low-temperature resistance and composite pollutant degradation capacity, strain XJJ-1 has the potential application prospects in the in-situ bioremediation of CB-contaminated sites.
研究领域:
环境科学
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微生物学
·
生物修复
·
生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Kiran Gupta,Baoling Yuan,Chen Chen,Naresh Varnakavi,Ming-Lai Fu
Novel composite of immobilized layered K 2x Mn x Sn 3−x S 6 (x = 0.5–0.95) (KMS-1) on the surface of reduced graphene oxide (r-GO), KMS-1/r-GO (KGO), has been fabricated by employing hydrothermal technique and used for the removal of radioactive Cs + and Sr 2+ ions from aqueous solutions. The KGO was characterized thoroughly with different characteristic techniques and found that the laminar KMS-1 nanoplate were immobilized on the surface of r-GO nanosheets, forming a highly porous stacked structure. Batch studies have been done for obtaining optimal experimental parameters and investigating the effect of adsorption time, initial concentration of pollutant, pH of solution, temperature, and competing anions on the removal properties. At room temperature, the maximum adsorption capacity of KGO was 338.18 mg·g −1 and 220.12 mg·g −1 for both Cs + and Sr 2+ , respectively. Langmuir isotherm and pseudo-second-order kinetic models were fitted well with experimental data of removed Cs + and Sr 2+ . Adsorption showed good selectivity of composite towards Cs + and Sr 2+ in the presence of co-existing ions (Ca 2+ and Mg 2+ ). The proposed adsorption mechanism involved ion exchange through the presence of K + ions in KMS-1 and formation of surface ionic interactions between r-GO and ions through COO X or S X (X denoted Cs + and Sr 2+ ). This study highlighted the facile applicability of novel material KGO and could be used as a new adsorbent for the removal of radioactive ions.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
VIROLOGICA SINICA
·
2012
·Vol. 27
·期 1
·19-25
·
IF 0
Liang Yuan-Yuan,Wang Bin,Qian Dong-Meng,Li Ling,Wang Zhi-Hao,Hu Ming,Song Xu-Xia
To investigate the inhibitory effects of Ginsenoside Rb1 (GRb1) on apoptosis caused by Herpes Simplex Virus-1(HSV-1) in Human Glioma Cells (U251), U251 cells were infected by HSV-1 at a multiplicity of infection of 5 and GRb1, GRb1+HSV-1, HSV-1 and control groups. MTT and cell apoptosis assays were used to detect the inhibitory effects of GRb1 on the apoptosis of U251 cells that caused by HSV-1 infection for various concentrations of drug and virus treatments by MTT assay. We found that in the 400 μg/mL GRb1 and 400 μg/mL GRb1+HSV-1 groups, MTT values were higher than control group at all times ( P <0. 05). Moreover, the apoptosis rate in the 400 μg/mL GRb1+HSV-1 group was lower than the HSV-1 group ( P <0. 05). These results confirmed that, at appropriate concentrations, GRb1 could inhibit nerve cell apoptosis in HSV-1 infections.
研究领域:
医学
·
病毒学
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神经科学
·
药理学
✓ 已验证
DOI
PubMed
Frontiers in Plant Science
·
2019
·
IF 4.106
Hussain Sajid,Bai Zhigang,Huang Jie,Cao Xiaochuang,Zhu Lianfeng,Zhu Chunquan,Khaskheli Maqsood Ahmed,Zhong Chu,Jin Qianyu,Zhang Junhua
Salt stress in soil is a critical constraint that affects the production of rice. Salt stress hinders plant growth through osmotic stress, ionic stress, and a hormonal imbalance (especially ethylene), therefore, thoughtful efforts are needed to devise salt tolerance management strategies. 1-Methylcyclopropene (1-MCP) is an ethylene action inhibitor, which could significantly reduce ethylene production in crops and fruits. However, 1-MCPs response to the physiological, biochemical and antioxidant features of rice under salt stress, are not clear. The present study analyzed whether 1-MCP could modulate salt tolerance for different rice cultivars. Pot culture experiments were conducted in a greenhouse in 2016–2017. Two rice cultivars, Nipponbare (NPBA) and Liangyoupeijiu (LYP9) were used in this trial. The salt stress included four salt levels, 0 g NaCl/kg dry soil (control, CK), 1.5 g NaCl/ kg dry soil (Low Salt stress, LS), 4.5 g NaCl/kg dry soil (Medium Salt stress, MS), and 7.5 g NaCl/kg dry soil (Heavy Salt stress, HS). Two 1-MCP levels, 0 g (CT) and 0.04 g/pot (1-MCP) were applied at the rice booting stage in 2016 and 2017. The results showed that applying 1-MCP significantly reduced ethylene production in rice spikelets from LYP9 and NPBA by 40.2 and 23.9% (CK), 44.3 and 28.6% (LS), 28 and 25.9% (MS), respectively. Rice seedlings for NPBA died under the HS level, while application of 1-MCP reduced the ethylene production in spikelets for LYP9 by 27.4% compared with those that received no 1-MCP treatment. Applying 1-MCP improved the photosynthesis rate and SPAD value in rice leaves for both cultivars. 1-MCP enhanced the superoxide dismutase production, protein synthesis, chlorophyll contents (chl a, b, carotenoids), and decreased malondialdehyde, H2O2, and proline accumulation in rice leaves. Application of 1-MCP also modulated the aboveground biomass, and grain yield for LYP9 and NPBA by 19.4 and 15.1% (CK), 30.3 and 24% (LS), 26.4 and 55.4% (MS), respectively, and 34.5% (HS) for LYP9 compared with those that received no 1-MCP treatment. However, LYP9 displayed a better tolerance than NPBA. The results revealed that 1-MCP could be employed to modulate physiology, biochemical, and antioxidant activities in rice plants, at different levels of salt stress, as a salt stress remedy.
研究领域:
植物生理学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
Reaction Chemistry & Engineering
·
2024
·Vol. 9
·期 3
·728-739
·
IF 3.4
Tongtong Wu,Weili Gao,Jiehe Zhang,Mingxu Hao,Shiyu Zhang,Haisheng Tao
As a typical antibiotic, tetracycline is ecotoxic and prone to bacterial resistance, producing superbugs and causing a public ecological crisis, thus raising the need to eliminate tetracycline from the aqueous environment. In this work, magnetic catalyst Fe3O4@HKUST-1 was prepared for catalyzing the degradation of tetracycline by persulfate. X-ray diffraction, scanning electron microscopy, and vibrating sample magnetometry were used to characterize the catalyst, and the results show that the catalyst had good crystallinity and magnetic properties. Tetracycline removal in the Fe3O4@HKUST-1/persulfate system reached 84.80%, and the catalyst was easily recovered from the solution due to its good magnetic properties. Mechanism analysis revealed that the non-radical pathway was the main degradation channel in the Fe3O4@HKUST-1/persulfate system, and 1O2 was the main reactive oxygen species generated from Ov on the surface of the Fe3O4@HKUST-1 catalyst. Tetracycline degradation was achieved by hydroxylation, demethylation, decarbonylation, dehydroxylation and the cleavage of C–N bonds. This finding holds significant value in further comprehension of the catalytic activation mechanism of metal–organic frameworks derivatives on persulfate and provides novel ideas for efficient water treatment technology development.
研究领域:
环境科学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Jingcheng Du,Changyuan Zhang,Hong Pu,Yufeng Li,Saimeng Jin,Luxi Tan,Cailong Zhou,Lichun Dong
Oil/water separation is an urgent topic because the frequently occurred oil leakages and increasing discharge of oily wastewater. The 3D metal foams with special wettabilities are promising oil/water separation materials owing to their outstanding properties. Herein, we fabricated a HKUST-1 MOFs decorated copper foam using a facile template-sacrificial method for oil/water separation. The as-prepared copper foam possessed good superhydrophobicity under broad pH and saline conditions. Its oil adsorption capability was larger than 150% for all the chosen oils and the oil/water separation efficiency of the foam for various oil/water mixture was larger than 96%. Furthermore, although the wettability decreased to hydrophobic state after 30 cycles of mechanical abrasion, the separation efficiency of the foam kept above 97%, showing good repeatability. Results demonstrated that the distinctive 3D structure of copper foam contributed the excellent durability to mechanical abrasion.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）