APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Qingdong Qin,Na Qiao,Yahong Liu,Xian Wu
The morphology and structure of heterogeneous catalysts play crucial roles in peroxymonosulfate (PMS) activation by providing much more available active sites and high chemical stability. In this work, spongelike porous CuO (SP-CuO) was synthesized and used as a PMS activator to degrade Acid Orange 7 (AO7) from aqueous solution. The morphology and property of SP-CuO were characterized using various techniques. The results showed that nanoparticles of SP-CuO with uniform in size were agglomerated together to form a porous spongelike texture. In the catalytic experiments, SP-CuO showed highly effective in heterogeneous activation of PMS compared to commercial CuO and Co 3 O 4 . The first-order rate constant of SP-CuO was approximately 3.9 and 4.4 times greater than that of commercial CuO and Co 3 O 4 , respectively. The effects of experimental parameters including PMS dose, AO7 concentration, catalyst dosage, temperature and pH were systematically investigated. The highest degradation rate was achieved at pH 7.0. The activation energy of SP-CuO was obtained as 21.6 kJ mol −1 . Sulfate radical (SO 4 − ), mainly generated through heterogeneous reaction, was the dominant reactive species for AO7 degradation. Moreover, SP-CuO remained good activity and stability after being recycled five times, demonstrating its potential environmental application in the treatment of dye wastewater.
研究领域:
环境科学
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催化
·
水处理
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产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
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2016
·
IF 3.485
Tao Chen,Xiaohua Han,Zhi Peng,Aihua Li,Jingquan Liu
7, 8-Dihydroxyflavone (7, 8-DHF) is an effective drug for the treatment of Parkinson’s disease. However, its application is heavily limited by its poor water solubility. To improve its hydrophilicity, biocompatible poly (ethylene glycol)acrylate (PEG-A) was grafted onto 7, 8-DHF via in situ reversible addition-fragmentation chain transfer (RAFT) polymerization, forming a kind of a drug/polymer composite, DHF/PPEG-A. It was found that the solubility of DHF/PPEG-A was significantly improved by 78 times compared to the unmodified precursor. Impressively, both the cytotoxicity and the drug efficacy of the synthesized DHF/PPEG-A just slightly decreased compared with the unmodified 7, 8-DHF. Moreover, the drug efficacy of DHF/PPEG-A can be regained by the hydrolysis in the presence of the esterase D due to the decomposition to 7, 8-DHF in body. This dramatical improvement of the water solubility of DHF/PPEG-A and the insignificant compromise of its drug cytotoxicity could envision extensive practical clinical administration of 7, 8-DHF for enhanced drug efficacy.
研究领域:
药学
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材料科学
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生物医学工程
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产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 149
·期 4
·1160-1168
·
IF 4.2
Yahui Zhang,Haifeng Yao,Yongping Dong
The poor solubility of iridium complexes severely hampers its application in the electrochemiluminescence (ECL) sensing field. The doping of an iridium complex with silica (SiO2) nanospheres provides feasible solution for this problem. Herein, one kind of water insoluble iridium complex ([Ir(dFppy)2(d(CF3)bpy)](PF6)) was doped with SiO2 nanoparticles to obtain a luminescent nanocomposite (SiO2@Ir) which could generate strong ECL signals to act as a beacon molecule. An “off–on–off” mode ECL sensor was proposed based on the competitive host–guest interactions between 2-adamantanamine (2-ADA), ferrocene (Fc) and cucurbit[7]uril (Q[7]). Fc could be used as an inhibitor to decrease the ECL signal, while Q[7] could wrap Fc to recover the ECL signal. Q[7] has a stronger binding ability with 2-ADA than Fc. As a result, 2-ADA could replace Fc from the cavity of Q[7], and the ECL signal was inhibited again. Under the optimal conditions, the ECL intensity varied linearly with 2-ADA concentration in the range of 1.0 × 10−8 to 1.0 × 10−6 mol L−1 with a detection limit of 2.5 × 10−10 mol L−1. The results not only provided a new avenue for the application of water insoluble iridium complexes in ECL sensing, but also revealed the attractive potential of host–guest interactions in the fabrication of biosensors.
研究领域:
分析化学
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生物传感
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Chaojie Bai,Yang Gao,Zuoqun Zhang,Longdou Tu,Dajian Cai,Zixuan Lv,Congjie Gao,Lixin Xue
It is an important initiative to reduce the building energy consumption using energy recovering ventilation (ERV) systems. The application of ERV systems is hindered by the low CO2 barrier performance of commercial total heat exchange membranes (THEMs) that lead to unsatisfactory indoor air refreshing rate, and there is an urgent need for THEMs that have improved CO2 barrier properties and effective energy recovery efficiencies. Here, we report the formation of novel ZIF/PA TFN THEMs based on ZIF-7-X nanoparticles (NPs) with “core–shell” structures and tunable particle sizes, formed from benzimidazole (BIM) ligands and BIM substituted by −NH2, −CH3, −C2H5, and −C3H7 functional groups. The NPs were mixed with pyr omellitic triformyl chloride (TMC) in the organic phase during the interface polymerization process to form ZIF/PA TFN membranes. The total heat exchange performance of ZIF/PA TFN membranes could be effectively modified by the type and quantity of ZIF-7-X NPs added. The CO2 barrier properties and water vapor permeability of ZIF/PA TFN membranes could be improved by the addition of optimal levels of ZIF-7-X NPs, showing low CO2 permeance of 7.76 GPU, high H2O permeance of 663.8 GPU, and excellent enthalpy exchange efficiency of 72.1%. This work provided an effective strategy for tuning not only the nanostructures of ZIF-7 fillers but also the CO2 barrier properties of the formed ZIF/PA TFN membranes.
研究领域:
材料科学
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环境工程
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产品关联
DOI
PubMed
高影响因子
Journal of Breath Research
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2019
·Vol. 13
·期 3
·036005
·
IF 3
Yuanling Liu,Wenwen Li,Yixiang Duan
Oxidative stress (OS) refers to the process of oxidative damage caused by reactive oxygen species (ROS) and reactive nitrogen species (RNS) accumulated by the organisms or cells during the process of generating and scavenging oxygen free radicals. In this study, an OS model of MCF-7 cells was constructed through H2O2-treatment, which for the first time combined volatile organic compounds (VOCs) and intracellular metabolic analysis for a comprehensive and in-depth study of the OS effect on cell metabolism. The VOCs produced by cells in H2O2-treated groups and control groups were extracted with solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometer (GC-MS). Meanwhile, the intracellular metabolites were extracted by methanol and analyzed by GC-MS coupled with silyl-derivatization. The results indicated 15 VOCs and 29 intracellular metabolites were statistically different (p < 0.05) between the H2O2-treated groups and the control groups. The VOC biomarkers were mainly linear chain alkanes and branched chain alkanes, and they were up-regulated in cells treated by H2O2. The intracellular metabolites with significant changes were mainly fatty acids, amino acids, and organic acids. Most of them, however, were down-regulated under OS state. This study revealed volatile and non-volatile metabolites that influenced by elevated OS in MCF-7 cells, and provided clues for mechanism exploration of exhaled biomarkers, thus promoting the non-invasive prediction of BC.
研究领域:
癌症研究
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代谢组学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF DRUG TARGETING
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2016
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IF 2.821
Lijing Zhang,Hua Cao,Jiaxin Zhang,Chengli Yang,Tingting Hu,Huili Li,Wu Yang,Gu He,Xiangrong Song,Aiping Tong,Gang Guo,Rui Li,Yu Jiang,Jiyan Liu,Lulu Cai,Yu Zheng
Specific delivery of drugs to bone tissue is very challenging due to the architecture and structure of bone tissue. A seven-repeat sequence of aspartate, a representative bone-targeting oligopeptide, is preferentially used for targeted therapy for bone diseases. In this study, Asp7-cholesterol((Asp)7-CHOL) was synthesized and (Asp)7-CHOL-modified liposome loaded with doxorubicin (DOX) was successfully prepared using both pre-insertion (pre-L) and post-insertion (post-L) methods. The formulation was optimized according to particle size, zeta potential and the drug-loading efficiency of the liposome. In addition, the bone affinity of the (Asp)7-CHOL-modified liposome was evaluated using a hydroxyapatite (HA) absorption method. The results suggested that (Asp)7-CHOL-modified liposome show excellent HA absorption; pre-L showed slightly higher HA binding than post-L. However, post-L had a higher DOX entrapment efficiency than pre-L. In vivo imaging further demonstrated that pre-L showed a higher bone-targeting efficiency than post-L, which was consistent with in vitro results. In all, (Asp)7-CHOL-modified liposome showed excellent bone-targeting activity, suggesting their potential for use as a drug delivery system for bone disease-targeted therapies.
研究领域:
药学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
MOLECULAR AND CELLULAR PROBES
·
2019
·
IF 2.511
Jilian Su,Junmei Wang,Jiamin Luo,Haili Li
Aims Vascular endothelial growth factor (VEGF) can promote cell division, proliferation and migration. In this study, we aimed to investigate roles of ultrasound-mediated destruction of VEGF-targeted and paclitaxel (PTX)-loaded lipid microbubbles (VTPLLM + US) in human breast cancer cells . Methods The activity of MCF-7 cells was determined by cell counting Kit-8. Flow cytometry was performed to detect the cells apoptosis and cell cycle. The expression of cell cycle-associated proteins, matrix metalloprotein-9 (MMP-9), VEGF and apoptosis-associated proteins were detected by qRT-PCR and Western blot . Results The obtained data suggested that VTPLLM + US promoted the G1 phase cell cycle arrest and suppressed the viability of MCF-7 cells. We also found that VTPLLM + US accelerated cells apoptosis. Cell cycle-associated proteins and VEGF expression were modulated by VTPLLM + US. Moreover, VTPLLM + US was found to regulate the expression levels of apoptosis-associated proteins in MCF-7 cells. Our findings suggested that VTPLLM + US suppressed the proliferation and accelerated the apoptosis of MCF-7 cells through regulating VEGF expression. Conclusions The potential effects of VTPLLM + US on apoptosis of MCF-7 cells suggest that applying VTPLLM + US might be an effective strategy in breast cancer therapies.
研究领域:
肿瘤学
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药理学
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医学影像学
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癌症治疗
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 19
·2597-2604
·
IF 2.378
Wei Zhuang,Yining Li,Jiabi Chen,Weihui Liu,Hongyuan Huang
A simple and portable potentiometric immunoassay was designed for the quantitative detection of matrix metalloproteinase-7 (MMP7) in acute kidney injury and renal cancer on a copper ion-selective electrode (Cu-ISE). To construct such an assay system, a sandwich-type immunoreaction was initially carried out on monoclonal anti-MMP7 capture antibody-coated microplates by using an initiator DNA strand-labeled anti-MMP7 secondary antibody. Thereafter, a hybridization chain reaction (HCR) was used for the signal amplification between copper nanocluster-labeled two hairpin DNA probes. Accompanying the formation of the sandwiched immunocomplex, the labeled DNA initiator strand on the secondary antibody triggered the HCR between two alternating DNA hairpins to form a nicked double-helix with copper nanoclusters. The subsequent potentiometric measurement of copper ions released from the concatenated copper nanoclusters under acidic conditions was conducted on a portable handheld potentiometer. The immuno-HCR assay combined an easy-operation potentiometric assay with signal amplification by using an HCR-induced copper nanocluster concatemer. Under optimum conditions, the developed immuno-HCR assay exhibited a dynamic linear range of 0.01–100 ng mL−1 with a detection limit of 5.3 pg mL−1 and good reproducibility. Also, the immuno-HCR assay gave good anti-interfering capacity toward other biomarkers. The analysis for clinical human serum specimens revealed good accordance with the results obtained by an enzyme-linked immunosorbent assay. Importantly, our strategy is promising for enzyme-free and cost-effective analysis of low-abundance cancer biomarkers.
研究领域:
分析化学
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生物医学工程
·
临床化学
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产品关联
DOI
高影响因子
LIFE SCIENCES
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2023
·
IF 6.1
Haijie Wu,Xiaohu Shi,Yingda Zang,Xiaodi Zhao,Xikun Liu,Weida Wang,Wenying Shi,Clarence T.T. Wong,Li Sheng,Xiaoguang Chen,Sen Zhang
Aims Cisplatin is a widely-used drug in the clinical treatment of tumors, but kidney nephrotoxicity is one of the reasons that limits its widespread use. We previously found that 7-hydroxycoumarin-β-D-glucuronide (7-HCG) was one of metabolites of skimmin and highly enriched in the kidneys and maintained a high blood concentration in skimmin-treated rats. Therefore, we investigated whether 7-HCG has a protective effect on cisplatin-induced acute kidney injury. Materials and methods Male C57BL/6 mice were continuously administered 7-HCG for five days, and on the third day, an intraperitoneal injection of cisplatin was given to induce acute kidney injury. After 72 h, the mice were sacrificed for analysis. Serum and renal tissue were collected for renal function evaluation. RNA sequencing was used to explore mechanism, and further validated by western blot and immunohistochemistry. In addition, pharmacokinetic study of oral 7-HCG administration was performed to examine how much 7-hydroxycoumarin (7-HC) was metabolized and 7-HC possible effect on renal protection. Key findings 7-HCG significantly reduced serum BUN and SCR levels, and alleviated pathological damage in renal tissue, and reduced the renal index. RNA sequencing revealed that 7-HCG could reverse p38 MAPK regulation and apoptosis. By western blotting, it was found that 7-HCG could reduce renal injury by reducing p-p38, p-ERK, p-JNK, cleaved-caspase3 and Bax. The immunohistochemical results of cleaved-caspase3 were consistent with western blotting. 7-HCG also significantly reduced the production of ROS in kidney tissue. Pharmacokinetic experiments have shown that 7-HCG in the blood increased rapidly and was eliminated slowly, with an average t 1/2β of 18.3 h. And the concentration of 7-HCG in the target organ kidney was about 4 times higher than that in blood. Significance Our findings indicate that 7-HCG could exert its protective effect against cisplatin-induced acute kidney injury by inhibiting apoptosis via p38 MAPK regulation and elucidates its pharmacokinetics.
研究领域:
药理学
·
肾病学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wenqing Li,Shimin Zhang,Songchao Shi,Xiaoying Cheng,Chongwei An,Jingyu Wang,Baoyun Ye
Realizing the balance between high energy density and multifunctionality of energetic materials is a hot spot in current research, and its study is of great significance for the application of energetic materials. Regulating microstructure is widely recognized as an effective strategy for solving such problems. In this paper, four different microstructures of Hexanitrohexaazaisowurtzitane(CL-20)/1,1-diamino-2,2-dinitroethylene(FOX-7) composite microspheres, namely, hollow, porous irregular microspheres, porous microspheres, and layered porous microspheres, were prepared by microchannel recrystallization technology. The effects of crystal precipitation rate, binder mechanical properties, and purity of water phase on the microstructure of composite microspheres were systematically investigated. The four prepared samples were spherical particles with regular morphology and uniform particle size. The angle of repose and mechanical sensitivity tests showed that the CL-20/FOX-7 composite microspheres had good dispersibility and mechanical sensitivity. Ignition experiments show that hollow microspheres have the best energy release efficiency, while porous microspheres have higher energy density and more stable energy release efficiency. The results show that the combustion performance and safety of the composite microspheres can be effectively enhanced by adjusting the microstructure. Microchannel recrystallization technology can realize the controllable preparation of the microstructure of composite microspheres, which provides a reference for the design and preparation of other composite microspheres.
研究领域:
材料科学
·
能源化学
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产品关联
DOI
高影响因子
Bioresources and Bioprocessing
·
2016
·Vol. 3
·期 1
·1-8
·
IF 0
Zhang Xiao-Li,Zong Min-Hua,Li Ning
Enzymatic approaches have become promising alternatives to chemical methods for the production of semi-synthetic β-lactam antibiotics. In this work, enzymatic synthesis of N-bromoacetyl-7-aminocephalosporanic acid (N-bromoacetyl-7-ACA), the key intermediate for the production of cefathiamidine, was reported for the first time.Of the immobilized penicillin acylases (PAs) tested, PGA-750 was the best biocatalyst. Optimization of the biocatalytic process was conducted. The optimal acyl donor, molar ratio of acyl donor to 7-ACA, pH, temperature, 7-ACA concentration, and enzyme dosage were methyl bromoacetate, 3, 7.5, 20 °C, 50 mmol/L and 4 U/mL, respectively. Under the optimal conditions, enzymatic N-acylation of 7-ACA with methyl bromoacetate afforded the desired product with the yield of 85% in 2 h, where the synthesis/hydrolysis (S/H) ratio was approximately 1.5. The immobilized enzyme PGA-750 exhibited good operational stability, and the relative yields of approximately 90% and 63% were achieved, respectively, when it was reused in 7th and 11th batch.An enzymatic approach to N-bromoacetyl-7-ACA, the key intermediate for the industrial production of cefathiamidine, has been developed successfully in a fully aqueous medium. The present work may open up a novel opportunity for the production of cefathiamidine through a simple and green process.
研究领域:
生物化学
·
制药工程
·
酶催化
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产品关联
DOI
PubMed
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Xiaodan Wu,Chengdong Nie,Mengqiong Deng,Linan Sun,Ying Wang,Jingru Zhao,Yujie Fu,Yourong Duan,Ying Sun
In this study, natural polysaccharides were first modified to synthesize natural functional monomers (NFMs). Subsequently, a natural novel NFM-based imprinted hydrogel microsphere was prepared for the specific recognition and enrichment of 7-xylulose-10-deacetylpaclitaxel (7-X-10-DTA). Molecular dynamics computational simulations were employed to screen and compare the functional monomers. Subsequently, the optimal preparation and application conditions of NMIHM were determined, with a maximum adsorption capacity of 71.77 mg mL −1 . The adsorption behavior of NMIHM for 7-X-10-DTA conforms to the pseudo-second-order kinetic model and the Langmuir model for monolayer adsorption, representing a spontaneous exothermic process. When used as a solid-phase extraction filler, NMIHM increased the content of 7-X-10-DTA by 13.36 times after enrichment in Taxus × media Rehde. The 7-X-10-DTA extracted in this study exhibited low half-maximal inhibitory concentration (IC50) values of 0.3205 μM and 0.7636 μM against cervical cancer Hela cells and prostate cancer PC-3 cells, respectively, demonstrating a more potent killing effect on these two types of cancer cells. This research is expected to accelerate the research and development of natural functional monomers and the advancement of molecular imprinting technology, thereby promoting the rapid development of practical separation applications.
研究领域:
药学
·
生物化学
·
分离科学与技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）