JOURNAL OF NEUROSCIENCE
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2024
·
IF 4.4
Jing Chen,Yuan Gao,Shu-Ting Bao,Ying-Di Wang,Tao Jia,Cui Yin,Cheng Xiao,Chunyi Zhou
The comorbidity of chronic pain and depression poses tremendous challenges for the treatment of either one because they exacerbate each other with unknown mechanisms. As the posterior insular cortex (PIC) integrates multiple somatosensory and emotional information and is implicated in either chronic pain or depression, we hypothesize that the PIC and its projections may contribute to the pathophysiology of comorbid chronic pain and depression. We show that PIC neurons were readily activated by mechanical, thermal, aversive, and stressful and appetitive stimulation in naïve and neuropathic pain male mice subjected to spared nerve injury (SNI). Optogenetic activation of PIC neurons induced hyperalgesia and conditioned place aversion in naïve mice, whereas inhibition of these neurons led to analgesia, conditioned place preference (CPP), and antidepressant effect in both naïve and SNI mice. Combining neuronal tracing, optogenetics, and electrophysiological techniques, we found that the monosynaptic glutamatergic projections from the PIC to the basolateral amygdala (BLA) and the ventromedial nucleus in the thalamus (VM) mimicked PIC neurons in pain modulation in naïve mice; in SNI mice, both projections were enhanced accompanied by hyperactivity of PIC, BLA and VM neurons, and inhibition of these projections led to analgesia, CPP, and antidepressant-like effect. The present study suggests that potentiation of the PIC-BLA and PIC-VM projections may be important pathophysiological bases for hyperalgesia and depression-like behavior in neuropathic pain, and reversing the potentiation may be a promising therapeutic strategy for comorbid chronic pain and depression.Significance Statement Treatment of chronic pain is quite challenging because of the involvement of central sensitization and emotional disorders. It is keenly demanding to search for brain circuits commonly or separately contributing to hyperalgesia and emotional dysfunction in chronic pain. The PIC is involved in both pain and emotional processing. But, the relationship between the malfunction in the PIC and the comorbidity of chronic pain and depression has not been elucidated. Here, we demonstrate that in chronic pain, PIC neurons became hyperactive, and their projections to the BLA and the VM of the thalamus were augmented; these alterations may confer hyperalgesia and depression-like behaviors in chronic pain. Therefore, these pathways may be potential therapeutic targets for comorbid chronic pain and depression.
研究领域:
神经科学
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疼痛研究
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行为神经科学
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神经回路
✓ 已验证
DOI
PubMed
高影响因子
Journal of Integrative Agriculture
·
2024
·
IF 4.6
Jinxin Yu,Jiayi He,Xuefeng Zhang,Chuxiao Lin,Shiyan Liu,Xin Gong,Xinnian Zeng,Jiali Liu
To ensure the reliability of learned information, most insects require multiple intervals of experience before storing the information as Long-term memory (LTM), and this requirement has been validated in insects from the behavioral to the molecular level. Recent studies have shown that some insects can form LTM after a single experience, although the mechanisms underlying one-trial LTM formation are not well understood. Therefore, understanding the mechanisms underlying rapid learning and subsequent preference formation in insects is crucial. Here we show that the agricultural pest Bactrocera dorsalis can rapidly form LTM, which is dependent on protein synthesis, and that the formation of LTM requires high energy support at the cost of reduced survival. Furthermore, based on a liquid chromatography-mass spectrometry (LC-MS) metabolomics approach, we found that LTM-related processes are sequentially coupled to two processes for energy generation, the TCA cycle and oxidative phosphorylation. This was further confirmed by blocking these energy generation processes. Our results provide a theoretical basis for the development of behavioral modulators in oriental fruit flies that target energy generation intermediate metabolites, as well as a new perspective on the rapid formation of LTM in insects.
研究领域:
昆虫学
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分子生物学
·
代谢组学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 3
·413
·
IF 5.1
Xinyu Zhang,Pai Peng,Qianying Ma,Shance Niu,Shande Duan,Yimeng Zhang,Xinzhong Hu,Xiaolong Wang
Dendrobium officinale (DO) is a well-known medicinal and edible plant, yet its impact on the quality of noodles has been infrequently reported. In this study, DO was incorporated into multi-grain flour in varying proportions (0, 2, 4, 6, 8%) to prepare noodles, and their quality was assessed. The percentage increase in DO decreased the cooking loss, whiteness, appearance, and taste of the noodles while simultaneously enhancing their water absorption, adhesiveness, smoothness, and starch digestion resistance. Lower supplemental levels of DO (2–4%) facilitated the water absorption of protein and the formation of a dense and extensive protein network surrounding the partially gelatinized starch, which was characterized by higher relative crystallinity. The highest sensory score (77.4) and greatest content of slowly digestible starch content (38%) were observed in the noodles containing 4% DO. Conversely, higher percentages of DO (6–8%) diluted and compromised the protein network in the cooked noodles, leading to water migration from protein to starch. The excessive polysaccharides from DO tended to complex with fully gelatinized starch, promoting starch aggregation and interactions between starch and non-starch components. This ultimately resulted in the highest adhesiveness and resistant starch content (34%) in the cooked noodles with 8% DO. These findings provide a reference for enhancing noodle quality by regulating the amount of DO added, thereby promoting the application of DO in cereal-based food products.
研究领域:
食品科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Xunliang Huang,Weiqiang Tian,Hao Jiang,Yue Xing,Ya Pu,Zhongwei Liu,Song Yang,Zhensheng Kang,Lin Cai
BACKGROUND Fungal phytopathogens pose a significant threat to global crop yields. The overuse of pesticides leads to pesticide-resistant pathogens, environmental contamination and risks to human health. Consequently, there is an urgent need for effective and ecofriendly antifungal strategies to address these challenges. RESULTS Herein, ZnO-ZIF-8 is synthesized via an in situ growth method to integrate the antifungal property of zinc oxide (ZnO) and the control release property of zeolitic imidazolate framework (ZIF)-8. Results suggest that the inhibition rates of ZnO, ZIF-8 and ZnO-ZIF-8 against Alternaria alternata are 44.4%, 74.1% and 94.4%, respectively, indicating that ZnO-ZIF-8 exhibits the most potent antifungal activity. ZnO-ZIF-8 also causes distortion and fracture of mycelia, significantly impairing spore germination and inhibiting germ tube elongation. CONCLUSION The potential antifungal mechanisms of ZnO-ZIF-8 involve the disruption of fungal cell morphology and structure through the release of Zn 2+ , ultimately leading to fungal cell death. Biosafety assessments indicate that the application of ZnO-ZIF-8 exhibits negligible effects on tobacco seeds and plants. This study provides valuable insights for the effective management of plant fungal diseases and supports sustainable agricultural development. © 2025 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Horticulture Research
·
2024
·
IF 7.6
Yuan Ziyu,Li Gu,Zhang Huixian,Peng Zhaoxin,Ding Wenyu,Wen Huan,Zhou Hanxin,Zeng Jiwu,Chen Jiajing,Xu Juan
Citrus fruits have abundant flavonoid glycosides (FGs), an important class of natural functional and flavor components. However, there have been few reports about the modification of UDP-glycosyltransferases (UGTs) on flavonoids in citrus. Notably, in flavonoid biosynthesis, 7-O-glucosylation is the initial and essential step of glycosylation prior to the synthesis of flavanone disaccharides, the most abundant and iconic FGs in citrus fruits. Here, based on the accumulation of FGs observed at the very early fruit development stage of two pummelo varieties, we screened six novel flavonoid 7-O-glucosyltransferase genes (7GlcTs) via transcriptomic analysis and then characterized them in vitro. The results revealed that four Cg7GlcTs possess wide catalytic activities towards various flavonoid substrates, with CgUGT89AK1 exhibiting the highest catalytic efficiency. Transient overexpression of CgUGT90A31 and CgUGT89AK1 led to increases in FG synthesis in pummelo leaves. Interestingly, these two genes had conserved sequences and consistent functions across different germplasms. Moreover, CitUGT89AK1 was found to play a role in the response of citrus to Huanglongbing infection by promoting FG production. The findings improve our understanding of flavonoid 7-O-glucosylation by identifying the key genes, and may help improve the benefits of flavonoid biosynthesis for plants and humans in the future.
研究领域:
植物科学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2015
·
IF 3.574
Fengzhong Wang,Huifang Wang,Donghui Wang,Fang Fang,Jixiang Lai,Tao Wu,Rong Tsao
The changes in isoflavone and γ-aminobutyric (GABA) contents and antioxidant activity of germinated soybean seeds were investigated. Three Chinese soybean cultivars (ZH 13, 30 and 42) released for high yield and protein content were evaluated. The results showed that the total isoflavone contents at peak level were 86.3, 81.0, and 42.3% higher than that of the un-germinated control for ZH 42, 13 and 30, respectively. GABA content of ZH 13 in particular was a statistically significant 36.7-fold increase in day 5 germinated soybeans as compared to un-germinated soybean. Such increases also led to significantly elevated antioxidant activities . Moreover, germination can significantly suppress IL-8 expression in Caco-2 cells by ZH 13 extract ( P < 0.05) without cell apoptosis. Current results suggest that ZH 13 soybean is a cultivar that can be used to develop nutritional and functional foods with important health benefits.
研究领域:
食品科学
·
植物学
·
营养学
✓ 已验证
DOI
高影响因子
DEVELOPMENT
·
2024
·
IF 3.7
Yu Meng,Thorner Konrad,Parameswaran Sreeja,Wei Wei,Yu Chuyue,Lin Xinhua,Kopan Raphael,Hass Matthew R.
The conserved Runt-related (RUNX) transcription factor family are master regulators of developmental and regenerative processes. Runx1 and Runx2 are expressed in satellite cells (SC) and in skeletal myotubes. Conditional deletion of Runx1 in adult SC negatively impacted self-renewal and impaired skeletal muscle maintenance even though Runx2 expression persisted. Runx1 deletion in C2C12 cells that retain Runx2 expression identified unique molecular functions of Runx1 that cannot be compensated by Runx2. The reduced myoblast fusion in vitro caused by Runx1 loss was due in part to ectopic expression of Mef2c, a target repressed by Runx1 . Structure-function analysis demonstrated that the Ets-interacting MID/EID region of Runx1, absent from Runx2, is critical to Runx1 myoblast function and for Etv4 binding. Analysis of ChIP-seq datasets from Runx1 (T-cells, muscle) versus Runx2 (preosteoblasts) dependent tissues identified a composite Ets:Runx motif enriched in Runx1 -dependent tissues. The Ets:Runx composite motif was enriched in peaks open exclusively in ATAC-seq datasets from WT cells compared to ATAC peaks unique to Runx1KO cells. Thus, engagement of a set of targets by the RUNX1/ETS complex define the non-redundant functions of Runx1 .
研究领域:
分子生物学
·
细胞生物学
·
转录因子
·
肌肉再生
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 21
·13506
·
IF 6.208
Guobin Li,Lan Zhang,Zhe Lu,Baoqiang Yang,Hui Yang,Peng Shang,Jean X. Jiang,Dong’en Wang,Huiyun Xu
Osteoporosis and sarcopenia (termed “Osteosarcopenia”), the twin-aging diseases, are major contributors to reduced bone mass and muscle weakness in the elderly population. Connexin 43 (Cx43) in osteocytes has been previously reported to play vital roles in bone homeostasis and muscle function in mature mice. The Cx43-formed gap junctions (GJs) and hemichannels (HCs) in osteocytes are important portals for the exchange of small molecules in cell-to-cell and cell-to-extracellular matrix, respectively. However, the roles of Cx43-based GJs and HCs in both bone and muscle aging are still unclear. Here, we used two transgenic mouse models with overexpression of the dominant negative Cx43 mutants primarily in osteocytes driven by the 10-kb Dmp1 promoter, R76W mice (inhibited gap junctions but enhanced hemichannels) and Δ130–136 mice (both gap junction and hemichannels are inhibited), to determine the actions of Cx43-based hemichannels (HCs) and gap junctions (GJs) in the regulation of bone and skeletal muscle from aged mice (18 months) as compared with those from adult mice (10 months). We demonstrated that enhancement of Cx43 HCs reduces bone mass due to increased osteoclast surfaces while the impairment of Cx43 HCs increases osteocyte apoptosis in aged mice caused by reduced PGE2levels. Furthermore, altered mitochondrial homeostasis with reduced expression of Sirt-1, OPA-1, and Drp-1 resulted in excessive ROS level in muscle soleus (SL) of aged transgenic mice. In vitro, the impairment of Cx43 HCs in osteocytes from aged mice also promoted muscle collagen synthesis through activation of TGFβ/smad2/3 signaling because of reduced PGE2levels in the PO CM. These findings indicate that the enhancement of Cx43 HCs while GJs are inhibited reduces bone mass, and the impairment of Cx43 HCs inhibits PGE2level in osteocytes and this reduction promotes muscle collagen synthesis in skeletal muscle through activation of TGFβ/smad2/3 signaling, which together with increased ROS level contributes to reduced muscle force in aged mice.Keywords:osteocytes;hemichannels;gap junctions;bone-muscle crosstalk;aging
研究领域:
生物学
·
生理学
·
老年学
·
肌肉骨骼系统
✓ 已验证
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2021
·
IF 3.463
Xiaoya Tao,Qiong Wu,Jiayin Li,Di Wang,Sanusi Shamsudeen Nassarawa,Tiejin Ying
Phytohormone methyl jasmonate (MeJA) plays an important role in fruit ripening. This research aimed to investigate the regulation of MeJA in ethylene biosynthesis and signal transduction during postharvest tomatoes ripening. After mature green cherry tomatoes were infiltrated with MeJA (0.5 mM) or deionized water (control) respectively, fruit colour and firmness, ethylene production, the activities and expressions of ACS and ACO, as well as the expressions of major genes involved in ethylene signal transduction were periodically monitored. Results showed that a significant acceleration in ripening in MeJA-treated fruit was observed, along with the accelerated changes of ethylene production, the enhanced activities and expression levels of ACS and ACO. The expression levels of ETR3 , ETR4 , ETR6 , ETR7 , EIN2 , EIL2 , EIL3 , EIL4 and ERF1 were up-regulated (from 1.26- to 6.50-fold) by MeJA during tomatoes ripening. The expression of CTR1 , however, was down-regulated (from 0.23- to 0.54-fold). Moreover, the expression of EIL1 was positively regulated at the early ripening stage whereas negatively regulated at the late ripening stage (11.76-/0.39-fold), whereas the expressions of CTR3 and CTR4 showed the opposite expression patterns (0.15-/7.78-fold, 0.48-/2.00-fold). Results suggested the up-regulation of the genes associated with ethylene biosynthesis, as well as the up-regulated transcriptional levels of the most genes involved in ethylene signal transduction, leading to the increase of ethylene production, response and action, would be one of the major mechanisms of MeJA in accelerating postharvest tomatoes ripening.
研究领域:
植物学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2023
·
IF 3.7
Chunyu Yu,Xue Li,Xin Qiao,Zhiwei Zhang,Danyang Zhang,Hua Liu,Xue-Li Hao,Zhongzhi Wang,Hongpeng You,Liang Zhou
Fluorescent carbon quantum dots (r-CDs) were synthesized safely and readily by stirring at room temperature with a yield of 76.5%. The synthesized r-CDs exhibit bright green emission with an absolute quantum yield of 12.0% under the excitation of ultraviolet light. Within the pH range of 3–12, the r-CDs exhibit excellent fluorescence stability. Interestingly, the fluorescence intensity of r-CDs faded promptly when the pH value diminished to 2 and then regained when the pH value returned to 3. This means that r-CDs can be used as “on–off–on” fluorescent probes for pH sensing. The mechanism of this sensitive pH response behavior was discussed in detail, and a possible mechanism of protonation–deprotonation–protonation processes was proposed. In addition, we have proposed a green preparation route for eco-friendly filter paper using anticounterfeiting. Interestingly, the presence of Fe2+ significantly quenched the fluorescence of r-CDs. Experimental results demonstrated the r-CDs can also be used to detect the content of Fe2+ in water, and the detection limit was 8.82 μM/L.
研究领域:
化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jing Chen,Shuying Li,Ying Chen,Jiao Yang,Jianbin Dong,Xiaoquan Lu
5-Hydroxytryptamine (5-HT) is an important neurotransmitter, and its content in the human body is of great significance to human health. In this study, an l-cysteine-terminated triangular silver nanoplate loaded on a MXene (two-dimensional transition metal carbide or nitride) (Tri-AgNP/l-Cys/MXene) electrochemical sensor was used to detect 5-HT. As an electrically active amino acid with a sulfhydryl group, l-cysteine (l-Cys) forms a more stable Ag–S bond with silver nanoparticles, which can selectively substitute trisodium citrate (TSC) in TSC-capped triangular silver nanoplates (Tri-Ag-NP/TSC). Due to the good conductivity, biocompatibility, and large surface area, MXenes provide a good platform for loading Tri-AgNP/l-Cys. Under optimized conditions, the concentration range for detecting 5-HT with the sensor is 0.5–150 μM, and the limit of detection (LOD) is 0.08 μM (S/N = 3). For detecting 5-HT in actual serum samples, the sensor also showed a good recovery rate (95.38–102.3%), and the relative standard deviation was 2.2–3.4%.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Yinchen Dai,Wubing Chen,Baochun Guo,Xiao Li,Jibiao Guan,Lina Wang,Ming Zhang
Efficient and inexpensive electrocatalysts play an important role in electrolysis of water and hydrogen evolution reaction . The catalytic activity of electrocatalyst can be improved by adjusting the electronic structure and increasing the active center. In this study, Ni 2 P nanosheets were grown on carbon cloth through straightforward solvothermal, and tiny MoS 2 nanosheets were uniformly covered on Ni 2 P nanosheets by in-situ growth method to form heterogeneous electrocatalyst (Ni 2 P @ MoS 2 /CC). The results show that Ni 2 P @ MoS 2 /CC had more active sites than Ni 2 P/CC and MoS 2 /CC catalysts. In addition, the interface interaction based on heterogeneous structure promotes its charge transfer kinetics. In alkaline electrolyte , Ni 2 P @ MoS 2 /CC electrocatalyst had good HER performance. At a current density of 10 mA cm −2 , the overpotential was 99 mV, and the Tafel slope was 97 mV dec −1 . In addition, the catalyst showed excellent electrochemical stability, with no significant loss of activity after 2000 cyclic voltammetry tests and 50 h i-t tests.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）