MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Ruhao Wang,Mingjing Wang,Xu Hun
Coxsackievirus B3 (CVB3), a member of the genus Enterovirus, has high risks for incidence and mortality. Developing speedy and sensitive molecular diagnostics for CVB3 detection is challenging. Herein, a novel highly sensitive photoelectrochemical (PEC) biosensor based on nanocomposite (ZnSeNSs/AuNPs/BNNSs) modified electrode was fabricated. This biosensor relied on reverse transcription of CVB3 RNA and the rapid isothermal amplication by recombinase polymerase amplification (RPA). The amplified product was identified and linked to photoelectrode. Then biotin on the amplified product was connected to strepavidin-alkaline phosphatase (SA-ALP). The alkaline phosphatase catalyzed the hydrolysis of substrate 4-aminophenylphosphate monosodium (4-APP) to generate free 4-aminophenol which was a electron donor. An enhancing PEC signal was obtained. Therefore, a relationship between photocurrent response and CVB3 concentration was established. Under the optimal conditions, this PEC biosensor displayed a liner photocurrent response in the range of 0.1 fM ∼100 fM with a low detection limit of 30 aM. Furthermore, this strategy with satisfactory stability and selectivity provides an innovative way for CVB3 detection and lays a foundation for the research of portable devices for clinical sample processing and analysis in the further.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Shuyun Bi,Di Shao,Yue Yuan,Rui Zhao,Xu Li
A novel surface-enhanced Raman spectroscopy (SERS) method for the determination of nitrofurazone was developed using AuNPs/ γ -Al 2 O 3 nanoparticles protected by β -cyclodextrin ( β -CD) as substrate prepared in our lab. The optimum experimental conditions were obtained from single factor procedure and response surface modeling. A linear relationship ( I SERS = 508.96 c + 31987.87, c : nmol L −1 , R 2 = 0.996) between SERS intensity and the concentration of nitrofurazone in the range of 3.3 – 667.0 nmol L −1 was established, the limit of detection (LOD) was found at nmol L −1 level (0.37 nmol L −1 by 3 S 0 / S ). The selectivity for the method was studied by the influences of foreign substances on the determination. The recoveries and RSD (n = 5) for the six meat samples were 95.1 % – 104.5% and 2.4 % – 4.8% respectively, which suggesting that the new SERS method was successfully to detecting nitrofurazone.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Huanhuan Bao,Meifang Yuan,Chengui Xiao,Daofeng Liu,Weihua Lai
A signal-enhanced LFIA based on tyramine (TYR)-induced AuNPs aggregation has been developed for the sensitive detection of danofloxacin (DAN). In the model, the hydroxyl radical produced by HRP catalyzing H 2 O 2 can trigger the TYR-AuNPs to aggregate on the T or C line for enhancing the detection signal. The linear range of TYR-AuNPs LFIA was 0.25–5 ng mL −1 with the limit of detection (LOD) of 0.032 ng mL −1 , and the LOD was 8-fold lower than that of the traditional AuNPs LFIA (0.26 ng mL −1 ). The TYR-AuNPs LFIA could be used with the naked eyes to qualitatively detect DAN with a cut-off limit of 2.5 ng mL −1 , which was 4-fold lower than that of the traditional AuNPs LFIA (10 ng mL −1 ). The recoveries of TYR-AuNPs LFIA were 86.04–105.14% and 92.41–110.19%, with the coefficient of variation of 1.71–2.05% and 4.42–5.89% in chicken and pork, respectively.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2021
·Vol. 25
·期 4
·1383-1391
·
IF 2.647
Yang Hongmei,Hu Peiyu,Tang Jing,Cheng Ying,Wang Fang,Chen Zilin
A simple and sensitive bifunctional electrochemical aptasensor for detection of adenosine and thrombin has been developed using gold nanoparticles–electrochemically reduced graphene oxide (AuNPs-ERGO) composite film-modified electrode. Firstly, the reduced graphene oxide film and AuNPs were sequentially immobilized on glassy carbon electrode (GCE) surface. Secondly, thrombin aptamer was immobilized on the modified electrode. Finally, adenosine aptamer was hybridized with it to serve as a recognition element and methylene blue (MB) as electrochemical signal indicator. In the presence of adenosine or thrombin, the sensor recognized it and a conformational change was induced in aptamer, resulting in decrease of the peak current of MB. The linear relation between concentration of adenosine or thrombin and peak current of MB allowed quantification of them. Thanks to the special electronic characteristic of AuNPs-ERGO composite film, sensitivity of sensor was greatly improved. Under optimal conditions, the proposed aptasensor presented an excellent performance in a linear range of 25 nM to 750 nM for adenosine and 0.5 nM to 10 nM for thrombin. Detection limits were estimated to be 8.3 nM for adenosine and 0.17 nM for thrombin, respectively. Moreover, dual-analyte detection of adenosine and thrombin was achieved without potentially increasing the complexity and cost of the assay.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yu Liu,Fangfang Wang,Yawen Liu,Zhigang Zhang,Haiming Hu,Yiqing Wang,Lei Xiong,Junping Zheng,Hongtao Liu
Conventional ELISA methods have been widely used for disease diagnosis, yet the sensitivity and accuracy are still to be improved. Based on our label-free AuNPs-mediated plasmonic sensor, we developed an ultrasensitive plasmonic ELISA by integrating the plasmonic sensor and cascade amplification. In principle, alkaline phosphatase (ALP)-conjugated antibodies captured targets, and the ALP hydrolyzed 2-phospho-ascorbic acid (AA2P) into ascorbic acid (AA). Then, the released AA changed Fe 3+ into Fe 2+ , which further triggered Fe 2+ -catalyzed H 2 O 2 -preventing aggregation of AuNPs by oxidizing Cysteine (FeHOAuC). Finally, the target content was negatively related with the AuNPs aggregation and the subsequent color variation. As a proof-of-concept, this novel plasmonic ELISA exhibited an ultralow LOD (0.1 ng/mL, around 4.4 pM) and a detection range (0.25–8.0 ng/mL) on detecting neutrophil gelatinase-associated lipocalin (NGAL) within 120 min. The sensitivity was superior to those of conventional ELISA and plasmonic ELISA. In summary, our study provided a novel and sensitive plasmonic ELISA for proteinic biomarker diagnosis.
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ANALYTICAL BIOCHEMISTRY
·
2023
·
IF 2.9
Yan Xiong,Shu Zhu,Hua Zhao,Jin Li,Yanting Li,Tao Gong,Yanru Tao,Jiangling Hu,Hongmei Wang,Xinhui Jiang
For patients receiving organ transplants, monitoring the blood concentration of MPA can provide timely information on whether MPA has reached the effective therapeutic window to better function while reducing the incidence of rejection or adverse reactions. In this study, an electrochemical sensor for the detection of MPA was built using a nanocomposite made of CS-MWCNT and AuNPs. At the same time, the high performance liquid phase (HPLC) method for MPA was established and compared with this sensor. The surface morphology, structure, and roughness of the material on the electrode were characterized by scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FTIR), and atomic force microscopy (AFM). In addition, the electrochemical behavior of the modified electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The standard curve was obtained in blank plasma, not pure buffer solution. The peak current was linearly related to the MPA concentration in the linear range of 0.001–0.1 mM with a detection limit of 0.05 μM and good anti-interference ability. Moreover, the sensor was employed with success for the determination of MPA in rat plasma with good recovery. The electrochemical sensor presented here is eco-friendly, and sensitive, and offers a great possibility for practical applicability.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Binbin Zhou,Hao Xie,Sisi Zhou,Xingxin Sheng,Liang Chen,Ming Zhong
In this work, a highly sensitive and selective molecularly imprinted electrochemical sensor is exploited to detect zearalenone (ZEA) by the synergistic effect of reduced graphene nanoribbons (rGNRs) and gold nanoparticles (AuNPs). The oxidized GNRs are firstly produced by an improved Hummers’ oxidation method, and then reduced and modified together with AuNPs onto a glassy carbon electrode by electrodeposition technique to realize collaborative amplification of electrochemical signal. The molecularly imprinted polymer film with specific recognition sites can be generated on the modified electrode by electropolymerization. The effect of experimental conditions is systematically investigated to obtain the best detection performance. It is found that the constructed sensor shows a wide linear range of 1–500 ng·mL −1 for ZEA with a detection limit as low as 0.34 ng·mL −1 . Obviously, our constructed molecularly imprinted electrochemical sensor shows great potential in the application of precisely detecting ZEA in food.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-17
·
IF 10.2
Hong Yueze,Fang Qiuna,Bai Ting,Zhao Peiyi,Han Yuemei,Lin Quankui
Posterior capsular opacification (PCO) is the most common complication after cataract surgery. Present strategies can’t meet the clinical needs of long-term prevention. This research reports a novel intraocular lens (IOL) bulk material with high biocompatibility and synergistic therapy. Gold nanoparticles (AuNPs) doped MIL-101-NH 2 metal–organic frameworks (MOFs) (AuNPs@MIL) was firstly fabricated via in situ reductions. Then the functionalized MOFs were uniformly mixed with glycidyl methacrylate (GMA) and 2-(2-ethoxyethoxy) ethyl acrylate (EA) to form the nanoparticle doped polymer (AuNPs@MIL-PGE), and which was used to fabricate IOL bulk materials. The materials’ optical and mechanical properties with different mass contents of nanoparticles are investigated. Such bulk functionalized IOL material could efficiently remove residual human lens epithelial cells (HLECs) in the capsular bag in the short term, and can prevent PCO on demand in the long run by near-infrared illumination (NIR) action. In vivo and in vitro experiments demonstrate the biosafety of the material. The AuNPs@MIL-PGE exhibits excellent photothermal effects, which could inhibit cell proliferation under NIR and doesn’t cause pathological effects on the surrounding tissues. Such functionalized IOL can not only avoid the side effects of the antiproliferative drugs but also realize the enhanced PCO prevention in clinical practice.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Congyi Zhao,Yaxu Zhao,Lianqi Wang,Ke Lu,Wei Guo,Xiong Lu,Faming Gao
In this experimental study, a highly sensitive electrochemical acetylcholinesterase (AChE) biosensor for the detection of organophosphorus pesticides (OPs) based on three-dimensional α-Fe 2 O 3 nanoflowers decorated with Au nanoparticles (AuNPs) and carbon nanotube (CNTs) (α-Fe 2 O 3 NFs-AuNPs-CNTs) was developed. With the blooms structure, the α-Fe 2 O 3 NFs not only provided an excellent microenvironment for the loading of AChE, but also increased the contact specific surface area. Meanwhile, AuNPs dispersed uniformly on the α-Fe 2 O 3 nanosheets and CNTs further enhanced the conductivity of the nanocomposites. Under the optimized conditions, the biosensor was made to exhibit favorable linear relationship with the logarithm of the concentration of omethoate from 1 × 10 −13 -1 × 10 −7 M, with the detection limit of 1.9 × 10 −15 M. In addition, the biosensor also possessed good repeatability, stability and practicality, proved to be a valuable tool for the detection of pesticides in environment.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Weidan Na,Changjin Ou,Yijun Ming,Yuchong Gao,Lin Tian
Surface enhanced Raman spectroscopy (SERS) detection was not as accurate and reproducible as fluorometry due to some uncertainties such as operator's focus adjustment and difference between capillaries. Herein, the weak Raman signal (1640 cm −1 ) from water bending vibration is adopted as a benchmark to improve the practical applicability of Raman detection. Specifically, amygdalin and citrate sodium were applied as reducing and capping agent to synthesize dual-ligand gold nanoparticles (DL-AuNPs). Additionally, the cyano group of amygdalin can attach to the surface of DL-AuNPs and generate a primary SERS signal (2174 cm −1 ), which can be significantly enhanced by Cu(II) as DL-AuNPs aggregates. More importantly, the strong binding affinity between pyrophosphate ions (PPi) and Cu(II) redisperses DL-AuNPs and decreases the signal intensity, making the DL-AuNPs/Cu(II) SERS sensor promising for the detection of PPi. This proposed method presents a reliable SERS analysis with the stable water background calibration and broaden the application of SERS technique in aqueous solution target monitoring.
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Chemosensors
·
2023
·Vol. 11
·期 4
·209
·
IF 4.2
Jiazheng Wan,Guocheng Han
Lysozyme (Lyz) is found in animal and human bodily fluids, and is frequently utilized as a biomarker for various diseases. Even trace amounts of Lyz in food can potentially trigger adverse immune system reactions in sensitive individuals. Therefore, it is very important to monitor Lyz concentration in foods for safety. In this study, a simple and convenient electrochemical sensor for Lyz detection was prepared by modifying gold nanoparticles (AuNPs) and ferrocene dicarboxylic acid (Fc(COOH)2) on a glass carbon electrode (GCE), which was characterized fully by various electrochemical methods and field emission scanning electron microscope (FESEM). The proposed method utilized Fc(COOH)2as a probe and AuNPs as an electron transfer medium to improve the sensor’s current response performance. Under optimal conditions, the sensor was used to detect Lyz with a linear range from 0.10~0.70 mmol·L−1with a sensitivity of 50.55 μA·mM−1·cm−2, and a limit of detection (LOD) of 0.07 mmol·L−1. In the standard addition experiment of food samples (egg white), a total R.S.D. of less than 6.75% and an average recovery between 95.45% and 102.62% were obtained.Keywords:electrochemical sensors;ferrocene dicarboxylic;gold nanoparticles;lysozyme;amplifier unit
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TALANTA
·
2023
·
IF 6.1
Haotian Wu,Gang Zhang,Xiaozhan Yang
An electrochemical biosensor based on iron tetroxide (Fe 3 O 4 )/carboxylated carbon nanotubes (MWCNTs-COOH)/gold nanoparticles (AuNPs) was designed for the detection of alpha-fetoprotein (AFP), which is often used as an important indicator for the early clinical diagnosis of liver cancer markers. The Fe 3 O 4 /MWCNTs-COOH nanocomposite was synthesized by a solvothermal method and it combined with gold nanoparticles (AuNPs) deposited at the constant potential on a glassy carbon electrode to form Fe 3 O 4 /MWCNTs-COOH/AuNPs, which intensifies the electrical signal while the large active sites enable more stable immobilization of the AFP monoclonal antibodies on the electrode. The electrochemical performance of Fe 3 O 4 /MWCNTs-COOH/AuNPs was investigated in detail and the electrochemical response signal after the immune reaction with the AFP antigen-antibody was recorded. The peak current Ip of the response signal is linearly proportional to the lg c AFP in the range of 1 pg mL −1 –10 μg mL −1 , with a detection limit of 1.09034 pg mL −1 and good performance in clinical sample testing. The proposed sensor has shown great application and development potential in clinical medicine field.
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