Biosensors-Basel
·
2023
·Vol. 13
·期 12
·1004
·
IF 5.4
Siqi Wang,Xinru Dong,Jialu Li,Jialei Liu,Yifei Ruan,Yinqiang Xia
Nonspecific adsorption has always been a critical challenge for sensor detection; thus, an efficient and facile approach for fabricating antifouling sensors is highly desirable. Here, we developed an antifouling coating on sensor surfaces, conveniently made with a simple drip of phase-transited BSA (PTB) followed by a modification with a peanut allergen antibody, which unexpectedly provides synergistic antifouling properties in sensors. Atomic force microscopy and scanning electron microscopy were used to evaluate the surface evenness. Optimizations in terms of PTB modification time and concentrations were performed using surface plasmon resonance by measuring protein resistance capabilities. Compared to bare Au surfaces, the PTB-modified surfaces exhibited low adsorption against BSA (<10 ng/cm2) and good resistance against lysozyme (Lyz). After immobilizing antibodies, the antifouling performance of the sensor coatings had an obvious enhancement, with almost no BSA adsorption and low lysozyme adsorption. The target recognition was also analyzed to verify the good sensing performance of the antifouling sensor. This understanding of antibody synergy provides suggestions for the development of antifouling sensors.Keywords:SPR;antifouling;phase-transited BSA;antibody synergy
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Yan Huang,Peter Osei Boamah,Jianbo Gong,Qi Zhang,Mingqing Hua,Yuzhen Ye
The fusion of molecular and anatomical modalities facilitates more reliable and accurate detection in clinic. In this work, we prepared gadolinium (III) complex Gd-DTPA-FITC-CS 11 with magnetic resonance (MR) and fluorescence dual-modal imaging modalities. Gd-DTPA-FITC-CS 11 consisted of fluorescein isothiocyanate and low-molecular-weight chitosan (CS 11 ) units conjugated with gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). Gd-DTPA-FITC-CS 11 exhibited a higher longitudinal relaxivity (14.09 mM −1 s −1 ) than the clinical Gd-DTPA (3.85 mM −1 s −1 ). T 1 -weighted MR contrast enhancement was also demonstrated the comparability to Gd-DTPA at lower dosage. The binding with bovine serum albumin (BSA) was investigated. The fluorescence of BSA in the presence of Gd-DTPA-FITC-CS 11 was weakened due to static quenching mechanism. The conformation of BSA was slightly changed but α -helix was dominant. The binding was entropy-driven and spontaneous and the main contribution was hydrophobic interaction. Our results suggested the potential of Gd-DTPA-FITC-CS 11 as an MR/fluorescence dual-modal imaging contrast agent in improving the diagnostic sensitivity and accuracy.
研究领域:
医学影像学
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2022
·Vol. 9
·期 20
·2200281
·
IF 17.521
Liwen Hong,Gaoxian Chen,Zhengwei Cai,Hua Liu,Chen Zhang,Fei Wang,Zeyu Xiao,Jie Zhong,Lei Wang,Zhengting Wang,Wenguo Cui
Emerging evidence indicates that a vicious cycle between inflammation and microthrombosis catalyzes the pathogenesis of inflammatory bowel disease (IBD). Over-stimulated inflammation triggers a coagulation cascade and leads to microthrombosis, which further complicates the injury through tissue hypoxia and ischemia. Herein, an injectable protein hydrogel with anti-thrombosis and anti-inflammation competency is developed to impede this cycle, cross-linked by silver ion mediated metal-ligand coordination and electronic interaction with sulfhydryl functionalized bovine serum albumin and heparin, respectively. The ex vivo experiments show that the hydrogel, HEP-Ag-BSA, exhibits excellent self-healing ability, injectability, biocompatibility, and sustained drug release. HEP-Ag-BSA also demonstrates anti-coagulation and anti-inflammation abilities via coagulation analysis and lipopolysaccharide stimulation assay. The in vivo imaging confirms the longer retention time of HEP-Ag-BSA at inflammatory sites than in normal mucosa owing to electrostatic interactions. The in vivo study applying a mouse model with colitis also reveals that HEP-Ag-BSA can robustly inhibit inflammatory microthrombosis with reduced bleeding risk. This versatile protein hydrogel platform can definitively hinder the “inflammation and microthrombosis” cycle, providing a novel integrated approach against IBD.
研究领域:
药理学
·
生物医学工程
·
胃肠病学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Xueyi Guo,Weijia Wang,Ying Yang,Qinghua Tian,Yang Xiang,Yan Sun,Zhiming Bai
Magnetic nano capture agent (MNCA)-based magnetic separation can remove heavy metals from blood efficiently and safely. It has been considered as the next-generation therapy for heavy metal intoxication. In this work, we report the first use of MNCA to remove arsenic (As) with high efficiency from blood. MNCA with a flexible, non-covalent and biocompatible surface layer has been synthesized by packaging cetyltrimethylammonium bromide (CTAB) and bovine serum albumin (BSA) together with oleic acid coated magnetic iron oxide nanoparticles (MNP@OA) via Van der Waals force. This surface layer enhances arsenic anion diffusion from the surface to the binding sites, leading to increased arsenic removal performance of MNCA. The adsorption capacities of BSA and CTAB enfolded MNP@OA (MNP@CTAB-BSA) for As (III) and As (V) are 131.00 mg g −1 and 119.58 mg g −1 , respectively. 89.02% of As (III) and 81.78% of As (V) are removed from blood using MNP@CTAB-BSA, and the residual concentrations of As (III) and As (V) in blood decrease from 400.00 μg L −1 to 43.93 μg L −1 and 72.88 μg L −1 , respectively. The as-synthesized MNP@CTAB-BSA shows excellent biocompatibility. Under operation condition, only 0.80% of hemolysis and 1.64% of plasma protein adsorption are induced by MNP@CTAB-BSA, and the cell viability of peripheral blood mononuclear cells (PBMCs) is more than 95.53%. This study paves a fast, safe and effective way for arsenic intoxication therapy.
研究领域:
纳米技术
·
环境科学
·
毒理学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Jiali Gu,Yan Zhang,Hongfei Tong,Yang Liu,Laiyu Sun,Yajun Wang,Li Xiao
In the present work, mixed-mode chromatography adsorbents grafted with dextran of different molecular weight were prepared. Compared to non-grafted adsorbent (BA-6B), the morphology of dextran-grafted adsorbents (BA-6B-Ts) did not change significantly, while the water content ( ω ), porosity ( P ) and pore volume ( V ) decreased. Static adsorption, salt tolerance and dynamic adsorption behaviors of adsorbents BA-6B-Ts against BSA/IgG were studied. Compared to adsorbent BA-6B, adsorbents BA-6B-Ts had higher saturated adsorption capacity ( Q m ) toward BSA/IgG, especially for BA-6B-T20, the Q m values toward BSA and IgG increased by 32.8% and 39.5%, respectively. Adsorbents BA-6B-Ts showed enhanced salt tolerance toward BSA, and relatively weaker salt tolerance toward IgG. The relative adsorption capacity of BA-6B-T20 was 66.7% at 0.1 mol/L NaCl and 39.2% at 0.5 mol/L NaCl, higher than those of BA-6B. Moreover, for both BSA and IgG, the effective pore diffusivity ( D e ) of BA-6B-Ts was higher than that of BA-6B. D e values of BA-6B-T20 toward BSA and IgG increased by 77% and 139% compared to BA-6B, respectively. Dynamic binding capacities (DBCs) of BA-6B-Ts were also improved. DBC of BSA and IgG on BA-6B-T20 were 25.6% and 46.5% higher than those on BA-6B. The possible mechanisms for the above difference in adsorption behavior of dextran-grafted adsorbents toward BSA/IgG were discussed, providing some insights into protein adsorption and mass transport for dextran-grafted MMC resins.
研究领域:
生物化学
·
分离科学
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 81
·66147-66154
·
IF 3.84
Xiaohong Zhu,Hui Li,Hui Zhou,Shian Zhong
A novel protein imprinted polymer, which combined the surface imprinting technology and magnetic halloysite nanotubes (MHNTs), was prepared for selective separation of a template protein. In this work, MHNTs were synthesized through the coprecipitation method based on encapsulation of Fe3O4 nanoparticles in halloysite nanotubes. The surface of the MHNTs was modified with vinyl groups through reaction with 3-(methacryloyloxy)propyl trimethoxysilane. Subsequently, the modified MHNTs were used as a support, N-isopropylacrylamide and methacrylic acid as bifunctional monomers, N,N′-methylene bisacrylamide as the crosslinking agent, and ammonium persulphate and N,N,N′,N′-tetramethylethylenediamine as initiators. The resulting polymer was named MHNTs@BSA-MIP, which was characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometry. The MHNTs@BSA-MIP exhibited high adsorption (48.4 mg g−1), good selectivity, rapid kinetic binding (45 min), fast magnetic separation (10 s), and favorable reproducibility (relative standard deviation <8% for batch-to-batch evaluation). The prepared MHNTs@BSA-MIP is suitable for separation and provides a significant reference for other proteins in proteomics.
研究领域:
材料科学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 22
·4214-4220
·
IF 4.543
Zulei Zhang,Xingdi Zhang,Dechao Niu,Yongsheng Li,Jianlin Shi
Natural antibodies are used widely for various applications such as in biomedical analysis, protein separation, and targeted-drug delivery, but they suffer from high cost and low stability. In this study, we developed a facile approach for the construction of antibody-like binding sites in a porous silica solid for efficient separation of bovine serum albumin (BSA) based on large-pore silica particles (LPSPs). This was accomplished by grafting two types of organosilane monomers, 3-aminopropyltriethoxylsilane (APTES) and octyltrimethoxysilane (OTMS), to provide hydrogen bonds or hydrophobic interactions with BSA through molecular imprinting technology. The resulting molecularly imprinted, large-pore silica particles (MI-LPSPs) were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TG), X-ray diffraction (XRD) and N2 sorption analysis. Results showed that the as-synthesized MI-LPSPs exhibited a spherical morphology, favorable stability and large pore structure. The kinetic adsorption experiments showed that the MI-LPSPs could reach equilibrium within one hour and were described well by the pseudo second-order model, indicating that chemical adsorption might be the rate-limiting step. Meanwhile, the MI-LPSPs had a large binding capacity up to 162.82 mg g−1 and high selectivity for the recognition of BSA. Moreover, such a high binding capacity and selectivity was retained after six runs, indicating a good stability and reusability of MI-LPSPs. Thus, it is expected that a simple synthetic methodology in the present study provides a promising pathway to prepare novel imprinted materials for efficient purification and separation of target proteins.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 8
·770
·
IF 5.4
Lei Deng,Feng Huang,Aomei Zhang,Tingting Wang,Minghui Yang,Xiaoqing Li,Xiang Chen
Halide lead perovskite has attracted increased attention due to its excellent optical properties. However, the poor stability of the halide lead perovskite nanocrystals has been a major obstacle to their application in biosensing. Here, we proposed a method to synthesize CsPbBr3/BSA NCs perovskite using bovine serum albumin (BSA) as a zwitterion ligand. Then, a fluorescent sensor for alkaline phosphatase determination based on CsPbBr3/BSA NCs was successfully built via the interaction of L-ascorbic acid (AA) with BSA on the perovskite surface. Under optimal conditions, the sensor showed a linear concentration range from 50 to 500 μM with a detection limit of 28 μM (signal-to-noise ratio of 3) for AA, and demonstrated a linear concentration range from 40 to 500 U/L with a detection limit of 15.5 U/L (signal-to-noise ratio of 3) for alkaline phosphatase (ALP). In addition, the proposed fluorescent biosensor exhibited good selectivity and recovery in the determination of ALP in human serum. This strategy offers an innovative way for enhancing the water stability of lead halide perovskite and promoting their application in biosensing areas.Keywords:perovskite;fluorescence sensing;alkaline phosphatase;L-ascorbic acid
研究领域:
材料科学
·
分析化学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Jianmin Li,Wenyi Zhang,Shuang Liu,Fan Yang,Yupeng Zhou,Lin Cao,Yiming Li,Yunfei Guo,Xiang Qi,Guoping Xu,Jing Peng,Yang Zhao
Purpose BSA-biomineralized Gd nanoparticles (Gd@BSA NPs) have been recognized as promising nanoscale MR contrast agents. The aim of this study was to carry out a preclinical evaluation of these NPs in a middle-sized animal model (rabbits).Methods New Zealand white rabbits were treated intravenously with Gd@BSA NPs (0.02 mmol Gd/kg) via a clinically-used high-pressure injector, with commercial Gd-diethylene triamine pentaacetate (Gd-DTPA)-injected group as control. Then MR angiography was performed according to the standard clinical protocol with a 3.0-T MR scanner. The SNR and CNR of the main arteries and branches were monitored. Pharmacokinetics and bioclearance were continuously evaluated in blood, urine, and feces. Gd deposition in vital organs was measured by ICP‒MS. Weight monitoring, HE staining, and blood biochemical analysis were also performed to comprehensively estimate systemic toxicity.Results The ultrasmall Gd@BSA NPs (<6 nm) exhibited high stability and T1 relaxivity. Compared to Gd-DTPA, Gd@BSA NPs demonstrated superior vascular system imaging performance at ultralow doses, especially of the cardiac artery and other main branches, and exhibited a significantly higher SNR and CNR. Notably, the Gd@BSA NPs showed a shorter half-life in blood, less retention in organs, and improved biocompatibility.Conclusion The preclinical evaluations here demonstrated that Gd@BSA NPs are promising and advantageous MR CA candidates that can be used at a low dose with excellent MR imaging performance, thus suggesting its further clinical trials and applications.
研究领域:
医学成像
·
纳米技术
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Shujun Huang,Xinyan Chen,Yu Lei,Wanting Zhao,Jipeng Yan,Jian Sun
Laccase is a very promising natural enzyme in the application of biochemical industry, but its application is severely limited by its high cost and poor stability. To overcome the inherent defects of natural laccase, here, we prepared a new type of small-size mimicking nanozymes based on self-assembly of bovine serum albumin (BSA) and copper sulfate (CuSO 4 ) with help of ionic liquid (IL). The effects of different ILs on the catalytic activity of the product (BSA-Cu nanozymes) were investigated, and it was found that BSA-Cu nanozymes with stable structures could be obtained in tetrabutylammonium hydroxide (TBAOH). Compared with laccase, the V max /K m of BSA-Cu nanozymes prepared by TBAOH was higher (0.081) than that of laccase (0.029), and the stability of BSA-Cu nanozymes were significantly enhanced in terms of pH, temperature, and ionic strength. The BSA-Cu nanozymes were utilized for detection and degradation of a series of phenolic substances, among which catechol was the most effective. In the detection of epinephrine, BSA-Cu nanozymes achieved a lower detection limit (0.38 µg/mL) and were more sensitive than laccase (3.53 µg/mL). The application of ILs can improve the mimicking laccase nanozymes performance and has great potential for further industrial application.
研究领域:
生物化学
·
环境科学
·
纳米技术
·
催化
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zhi Chen,Jian Sun,Guoliang Cheng,Yifan Wang,Huanhuan Liu,Hui Lin
Fluorescence assay for in situ and on-site monitoring of pyrethroid pesticides residues from plants is of great value but remains little research. In this study, a fluorescence biosensor Au NCs@BSA, is designed and constructed by embedding Au nanoclusters (Au NCs) in bovine serum albumin (BSA) to transmit on-the-scene cypermethrin pesticide residues messages. Harnessing orange emission and stimuli-responsive performance of Au NCs, as well as the specific strong binding ability of BSA to cypermethrin molecules, Au NCs@BSA is endowed with quantitative response to cypermethrin at μM level within 20 s through promoting fluorescence recovery in conjunction with the transformation of fluorescence images into digital signals. In addition, the interference experiment using common ions and other types of pesticides as coexisting interference substances demonstrated that the sensing system possesses brilliant selectivity and anti-interference ability. By combining the smartphone-based platform and convolutional neural network (CNN-model), different concentrations levels of cypermethrin were accurately identified in real samples. This study opens up a new avenue to engineer simple fluorescence assay for in situ and on-site capturing of the pyrethroid pesticide residue information, favoring the rapid development of agriculture food monitoring.
研究领域:
分析化学
·
食品安全
·
纳米生物技术
·
环境监测
·
农业科学
✓ 已验证
DOI
高影响因子
ACS Nano
·
2024
·
IF 15.8
Quan Zou,Xi Chen,Bingjie Li,Ruijie Zhang,Jinbin Pan,Xuejun Zhang,Xuening Zhang,Shao-Kai Sun
Contrast-enhanced magnetic resonance imaging (CE-MRI) of acute kidney injury (AKI) is severely hindered by the poor targeting capacity and potential toxicity of current contrast agents. Herein, we propose one-step fabrication of a bovine serum albumin@polydopamine@Fe (BSA@PDA@Fe, BPFe) nanoprobe with self-purification capacity for targeted CE-MRI of AKI. BSA endows the BPFe nanoprobe with renal tubule-targeting ability, and PDA is capable of completely inhibiting the intrinsic metal-induced reactive oxygen species (ROS), which are always involved in Fe/Mn-based agents. The as-prepared nanoprobe owns a tiny size of 2.7 nm, excellent solubility, good T1 MRI ability, superior biocompatibility, and powerful antioxidant capacity. In vivo CE-MRI shows that the BPFe nanoprobe can accumulate in the renal cortex due to the reabsorption effect toward the serum albumin. In the AKI model, impaired renal reabsorption function can be effortlessly detected via the diminishment of renal cortical signal enhancement. More importantly, the administration of the BPFe nanoprobe would not aggravate renal damage of AKI due to the outstanding self-purification capacity. Besides, the BPFe nanoprobe is employed for CE-MR angiography to visualize fine vessel structures. This work provides an MRI contrast agent with good biosafety and targeting ability for CE-MRI of kidney diseases.
研究领域:
医学成像
·
纳米技术
·
材料科学
·
生物医学工程
·
肾脏生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）