Acta Pharmaceutica Sinica B
·
2025
·
IF 14.6
Qian Jing,Mengnan Zhao,Yan Tang,Tao Chen,Mingyan Sun,Dandan Mi,Lan Zou,Rujing Wang,Jun Lu,Sanjun Shi
Interference with calcium homeostasis provokes tumor cell death and immune response, providing a novel direction for tumor immunotherapy as a promising cancer treatment strategy. Nevertheless, most reported Ca 2+ -overloaded nanoinducers encounter challenges such as intricate preparation procedures, safety concerns arising from inorganic material input, and limited anti-tumor efficiency. Herein, we synthesized a biocompatible and pH-sensitive Ca-doped cyclodextrin metal-organic framework (Ca/K-MOF) as a carrier, which was then loaded with photosensitizer hypericin (HY) via a simple one-pot synthesis to form HY@Ca/K-MOF. To enhance the stability both in vitro and in vivo , we coated HY@Ca/K-MOF with a hydrophilic layer of PEG ( PEG HY@Ca/K-MOF). When exposed to 590 nm photoirradiation, PEG HY@Ca/K-MOF, with its pH-responsive dissociation, the Ca 2+ and HY mediators released at the tumor site share the responsibility of triggering intracellular Ca 2+ disturbances, which amplified the production of reactive oxygen species (ROS) and led to mitochondrial calcium overload through modulating mitochondrial MICU1 function. Under photocontrol, this interplay between ROS generation and mitochondrial calcium overload created a bidirectional amplification effect, where each process reinforced the other, subsequently eliciting a pyroptosis-evoked immune response. Significantly, this newly constructed delivery platform effectively suppressed both primary and distant tumors without the need for additional immunological interventions. In summary, this Ca 2+ -doped MOF-based nanomaterial provides a promising approach for efficient tumor photo-controlled mitochondrial Ca 2+ overload-pyroptosis immunotherapy.
研究领域:
纳米医学
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癌症免疫治疗
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生物无机化学
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光动力治疗
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分子生物学
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药物递送系统
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DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2023
·
IF 7.9
Meihao Peng,Jintao Li,Jie Zhou,Bowen Zhang,Jiaqing Liao,Di Yang,Yu Wang,Yixi Yang,Rui Li,Xue Tang,Qiuxia Lu,Qi Zhao
Background Asthma is a chronic inflammatory disease that is challenging to treat. Fritillaria unibracteata var. wabuensis (FUW) is the plant origin for the famous Chinese antitussive medicine Fritillaria Cirrhosae Bulbus. The total alkaloids of Fritillaria unibracteata var. wabuensis bulbus (TAs-FUW) have anti-inflammatory properties and may be used to treat asthma. Purpose To explore whether TAs-FUW have bioactivity against airway inflammation and a therapeutic effect on chronic asthma. Methods The alkaloids were extracted via ultrasonication in a cryogenic chloroform-methanol solution after ammonium-hydroxide percolation of the bulbus. UPLC-Q-TOF/MS was used to characterize the composition of TAs-FUW. An ovalbumin (OVA)-induced asthmatic mouse model was established. We used whole-body plethysmography, ELISA , western blotting , RT-qPCR, and histological analyses to assess the pulmonary pathological changes in these mice after TAs-FUW treatment . Additionally, TNF-α/IL-4–induced inflammation in BEAS-2B cells was used as an in vitro model, whereby the effects of various doses of TAs-FUW on the TRPV1/Ca 2+ -dependent NFAT-induced expression of TSLP were assessed. Stimulation and inhibition of TRPV1 receptors by capsaicin (CAP) and capsazepine (CPZ), respectively, were used to validate the effect of TAs-FUW. Results The UPLC-Q-TOF/MS analysis revealed that TAs-FUW mainly contain six compounds (peiminine, peimine, edpetiline, khasianine, peimisine, and sipeimine). TAs-FUW improved airway inflammation and obstruction, mucus secretion , collagen deposition, and leukocyte and macrophage infiltration, and downregulated TSLP by inhibiting the TRPV1/NFAT pathway in asthmatic mice. In vitro , the application of CPZ demonstrated that the TRPV1 channel is involved in TNF-α/IL-4–mediated regulation of TSLP. TAs-FUW suppressed TNF-α/IL-4–induced TSLP generation expression by regulating the TRPV1/Ca 2+ /NFAT pathway. Furthermore, TAs-FUW reduced CAP-induced TSLP release by inhibiting TRPV1 activation. Notably, sipeimine and edpetiline each were sufficient to block the TRPV1-mediated Ca 2+ influx. Conclusion Our study is the first to demonstrate that TNF-α/IL-4 can activate the TRPV1 channel. TAs-FUW can alleviate asthmatic inflammation by suppressing the TRPV1 pathway and thereby preventing the increase in cellular Ca 2+ influx and the subsequent NFAT activation. The alkaloids in FUW may be used for complementary or alternative therapies in asthma.
研究领域:
药理学
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免疫学
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呼吸医学
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植物科学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
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IF 13.2
Haiyan Yang,Wenbo Han,Chun Shi,Dongtian Miao,Dawei Wang,Zhengjun Shi,Jing Yang,Jingjie Shen
Conventional conductive hydrogels face persistent sustainability challenges including energy-intensive fabrication processes, poor subzero temperature tolerance, and rapid dehydration under ambient conditions, contributing to electronic waste accumulation. To address these limitations, we developed an eco-friendly CaTPD hydrogel through rapid room-temperature polymerization initiated by a tannic acid-Ca 2+ complex, eliminating external energy requirements. The synthesis incorporates renewable biomass resources - dialdehyde cellulose derived from sustainable cellulose sources and plant-derived tannic acid - aligning with green chemistry principles. Structural characterization confirmed the formation of a porous network with coordinated complexes, yielding exceptional mechanical properties including 153 kPa tensile strength, 1423.1% fracture elongation, and 988.9 kJ/m 3 toughness. The incorporated calcium chloride imparted remarkable environmental stability, maintaining complete flexibility at −80 °C and preserving 81.5% of initial mass after 15 days at ambient conditions. The hydrogel demonstrated repeatable adhesion to diverse substrates (up to 32.3 kPa to wood) through multiple interfacial interactions. As a strain sensor, it achieved broad detection range (0–400% strain) with tunable sensitivity (gauge factor 1.63–16.87) and maintained signal stability over 3000 cycles. Practical applications were demonstrated through reliable monitoring of human motions ranging from large-scale joint movements to subtle swallowing actions. This work establishes a sustainable paradigm for flexible electronics by integrating biomass-derived materials with energy-efficient manufacturing, offering a viable solution to reduce energy consumption and electronic waste in sensor production while maintaining high performance under extreme conditions, ultimately contributing to cleaner production in the electronics industry.
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高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Yongbiao Sun,Jianfeng Tang,Guangyan Li,Yihuai Hua,Yunbing Sun,Suyang Hu,Xiaojin Wen
The Li + , K + , Mg 2+ and Ca 2+ -exchanged X zeolites were prepared using ion exchange method for producing efficient adsorbents that could be used in the purification of liquefied natural gas (LNG). The adsorption, separation and regeneration characteristics of the samples were systematically explored and discussed using static adsorption of pure gases, dynamic adsorption of a binary gas mixture (3%CO 2 and 97%CH 4 ) and regeneration experiments. The results showed that Li13X had the largest surface area of 520 m 2 /g, whereas Ca13X possessed the highest micropore volume of 0.25 m 3 /g with the smaller pore size observed from the pore size distribution . The adsorption capacity of Li13X for CO 2 (4.10 mmol/g) was almost the same as that of Ca13X (4.12 mmol/g), which were larger than all the other samples at 120 kPa and 30 °C. However, K13X had the highest static CO 2 /CH 4 separation factor of 46, which was due to the increased basicity of the framework. For the dynamic adsorption, Ca13X possessed the highest CO 2 /CH 4 separation factor of 93, and showed the smallest CH 4 adsorption capacity of 1.22 mmol/g. As for the regeneration of the proposed adsorbents, the heat requirement for CO 2 in dynamic was not only affected by the isosteric heat, but was also dependent on the competition between CH 4 and CO 2 . Moreover, the static and dynamic vacuum regenerations had the same tendency and mainly depended on the pore size distribution.
研究领域:
材料科学
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化学工程
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环境科学
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能源技术
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吸附分离
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DOI
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
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2022
·Vol. 129
·期 1
·69
·
IF 2.983
Deng Kaiyuan,Zhang Ruiting,Jin Yahong,Yuan Lifang,Wu Haoyi,Hu Yihua
A series of Mn 2+ -doped solid Zn 3 Ga 2 GeO 8 materials with far-red broad-band emissions is prepared as promising candidates for light emitting diodes (LEDs) for application in indoor plant cultivation. Phosphor exhibits broad far-red emissions, ranging from 600 to 1100 nm which overlap with the absorption range of the phytochrome ( \(P_{fr}\) ). In addition, the co-doping with Ca 2+ contributes a 1.57-fold increase in the far-red-emission intensity at room temperature. Encapsulating phosphor onto ultraviolet LED chips yields far-red-emitting LED devices, which can be promising candidates for promoting indoor plant cultivation.
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DOI
高影响因子
ACS Applied Engineering Materials
·
2023
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IF 0
Yu-cheng Zhang,Ya-qi Xue,Takayo Ogawa,Satoshi Wada,Jin-Ye Wang
Manufacturing biocompatible materials with higher-order structure has great significance because they can mimic the extracellular medium of the human organism and are a novel strategy for tissue regeneration. In this study, a device with stiffness-gradient characteristics based on two biocompatible materials, alginate with presolidification and photocurable acrylamide-containing supporting bath, was designed and constructed by the 3D printing technique. The presolidification can avoid rapid diffusion of alginate in aqueous solutions, improve mechanical properties without the introduction of heterogeneous gel precursor, and endow gradient stiffness by the controlled diffusion of calcium ions. Besides, a photocurable supporting bath was combined to manufacture a device with a dual-gradient structure by a 4-step procedure, including 3D printing, removal of the inner hydrogel, solidification of alginate, and curing of the supporting bath. A cylinder-like container was manufactured as the template, and the wall of the resultant container with two types of gradient structures showed parabola-like stiffness changes (open upward), resulting from calcium ion diffusion-controlled gradient solidification and alginate diffusion-controlled gradient photocuring. Moreover, the resultant device exhibited lower cytotoxicity to both adherent and suspension cells than containers manufactured with alginate. Because of the high water uptake of the photocured supporting bath, the removal of toxic metabolic products together with cell culture medium from the container leads to better cell compatibility. This diffusion-controlled device is also applicable to other additive manufacturers with biomedical significance.
研究领域:
生物材料
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组织工程
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增材制造
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细胞培养
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DOI
Physiological Reports
·
2026
·Vol. 14
·期 11
·e70975
·
IF 2.1
Yunxian Wang,Fenglan Chu,Yanhua Gong,Ruihong Guo,Hui Dong
Despite the ubiquitous use of monosodium glutamate (MSG) as a food additive, its specific effects on intestinal health and disease remain largely unclear. Here we found mucosal application of MSG induced an upward intestinal short-circuit current ( I sc ) in the murine ileum, which was significantly reduced by extracellular Cl − , Na + , and Ca 2+ removal. Mechanistically, MSG-induced I sc was significantly attenuated by pharmacological inhibition of key transporters (NCX, SGLT1, NKA) and secretory channels (CaCC, CFTR, IK Ca ). Furthermore, disrupting intracellular Ca 2+ homeostasis via endoplasmic reticulum (ER) chelation or blockade of IP 3 R, RyR, SOCE/CRAC, and VGCC significantly suppressed both the ileal I sc and MSG-stimulated Ca 2+ signaling in IECs. Importantly, MSG triggered a simultaneous elevation in intracellular Ca 2+ and a reduction in Na + , which were abolished by the blockers of NCX and SGLT1. In vivo, MSG-induced Ca 2+ -dependent ion transports were significantly reduced in irritable bowel syndrome with diarrhea (IBS-D) mice. Our findings indicate that MSG drives Ca 2+ -dependent intestinal ion transports through a complex network orchestrated by SGLT1 and NCX under healthy state, which is markedly attenuated in IBS-D. This study provides novel insights into the gastrointestinal impact of dietary MSG in both health and bowel disease.
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DOI
PubMed
高影响因子
Gels
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2026
·Vol. 12
·期 9
·791
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IF 6.4
Zixuan Zhang,Qianqian Wu,Hua Jiang
While poly (vinyl alcohol) (PVA)-based aerogels are promising porous carriers for controlled drug delivery, drying-induced pore collapse generally limits their internal pore accessibility, drug-loading capacity, and release regulation. In this study, duration-controlled tert-butanol (TBA) solvent exchange was developed as a pore-engineering strategy to regulate the structure-performance relationship of PVA/cellulose nanocrystal/Ca2+composite aerogels. This strategy focuses on balancing pore preservation, liquid-accessible pore connectivity, and network densification, instead of simply increasing the total surface area. With an optimized exchange duration, the TBA6 aerogel exhibited a well-preserved porous network. Moreover, its specific surface area and porosity increased from 33.11 to 96.02 m2·g−1and from 93.93% to 96.03%, respectively. This optimized structure enhanced salicylic acid (SA) loading, elevating the equilibrium adsorption capacity from 40.91 to 61.18 mg·g−1. Release kinetic analysis revealed that the Higuchi constant and the Korsmeyer-Peppas release constant decreased from 18.148 to 12.751 and from 25.070 to 17.283%·h−1/2, respectively, indicating a slower diffusion-regulated release process. Confocal Raman mapping further confirmed that TBA6 promoted a more homogeneous SA distribution within the aerogel matrix and enabled gradual release from the internal porous network. To sum up, controlling solvent-exchange duration is an effective strategy for tuning pore accessibility and diffusion resistance. This study provides new insight into the design of PVA-based composite aerogels for drug-loading and sustained-release applications.
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DOI
高影响因子
Journal of Advanced Research
·
2026
·
IF 17.1
Yi Wang,Laiming Zhang,Jiaxiong Wu,Ziyang Deng,Yunxuan Li,Xingqian Ye,Haibo Pan,Shiguo Chen
Introduction Phenolic compounds are regarded as promising α-glucosidase inhibitors for regulating postprandial blood glucose, but their α-glucosidase inhibitory activity in vivo is much lower than that in vitro . The small intestinal mucus layer serves as a barrier that impedes the binding of phenolic compounds to α-glucosidase, thereby attenuating their activity in vivo , yet the solution to this bottleneck remains unclear. Objectives Selecting prodelphinidin B digallate (proDB DG) as a representative phenolic compound, this study aimed to enhance its in vivo α-glucosidase inhibitory activity by screening the compound that can pre-regulate the barrier properties of the mucus layer. Methods The Caco-2/HT29-MTX-E12 co-culture model was used to screen the compound that can enhance the α-glucosidase inhibitory activity of proDB DG in vivo . This enhanced mechanism was analyzed using ultraviolet–visible light spectroscopy, zeta potential, rheological behavior, transmission electron microscopy, molecular dynamics simulation and Transwell mucus diffusion model. The improved α-glucosidase inhibitory activity and its biosafety were further confirmed by animal experiments. Moreover, the screened compound and proDB DG based tablets were developed. Results Ca 2+ improved the activity of proDB DG in lowering postprandial hyperglycemia in vivo . This enhancement of activity is attributed to its pre-regulation on the barrier properties of the mucus layer. Specifically, Ca 2+ -derived mucin aggregation increased the porosity and diffusivity of the mucus layer, thereby improving the permeability of proDB DG and facilitating its binding to α‑glucosidase. Ca 2+ also competitively bound with the mucus layer, and weakened the interaction between proDB DG and mucins. Furthermore, Ca 2+ –proDB DG based tablets possessed significant hypoglycemic effect and biosafety in vivo . Conclusion Ca 2+ regulates the barrier properties of the mucus layer, thereby promoting proDB DG to reach the mucosa quickly and then interact with α-glucosidase effectively, which may break the application limitations of proDB DG and even phenolic compounds as α-glucosidase inhibitors clinically.
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Cheng Wang,Hao Yu,QiHang Bu,ZiHan Wang,Ning Jiang,JiaoYang Chen,RongXue Sun,QianYuan Liu,JianHua Xu,JiaJun Fu
Given the environmental challenges caused by petroleum-based plastics, this study developed a novel green carboxymethyl chitosan (CMCS)-based active packaging composite film (CP) through a synergistic strategy of precise Ca 2+ coordination cross-linking and small-molecule plasticizing. Based on this strategy, the CP-4.5 film (CaCl 2 : 7 wt%, DL-3-phenyllactic acid (3-PLA): 4.5 wt%) exhibited exceptional mechanical properties, including high flexibility (Young's modulus: 0.747 GPa; elongation at break: 65.2 %) and high toughness (18.4 MJ m −3 ). The incorporation of CaCl 2 not only occupied voids within the CP film but also increased the physical cross-linking strength and density of the polymer network. This structural reorganization impeded the diffusion of O 2 and H 2 O molecules, reducing oxygen permeability by 85.9 % and water vapor permeability by 57.6 %. Additionally, 3-PLA, functioning as a broad-spectrum antibacterial agent, imparted the CP film with superior antibacterial activity. The CP film exhibited recyclability and repairability, and underwent complete biodegradation within 56 days. Overall, the synergistic effect of CaCl 2 and 3-PLA endowed the CP film with superior mechanical properties, barrier properties, antimicrobial activity, and environmental sustainability, effectively mitigating the quality deterioration of refrigerated grass carp fillets during storage, demonstrating the promising potential of the CP film for food packaging applications.
研究领域:
材料科学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lingyu Han,Nuo Dong,Jixin Yang,Bing Hu
The present study investigated the impact of Ca 2+ regulated sodium alginate gelation on buckwheat noodle digestion, as well as its physiological and biochemical effects on rat. An endogenous method was employed for noodle preparation, and the rate of starch hydrolysis was evaluated. Buckwheat noodles prepared using this approach exhibited significantly reduced rates of starch digestion compared to conventional methods. Increased concentrations of sodium alginate and Ca 2+ led to the formation of dense gel networks that promoted weight gain in diabetic rats while simultaneously lowering postprandial blood glucose levels and improving glucose intolerance and abnormal insulin tolerance. Moreover, these gel networks enhanced liver glycogen synthesis by increasing SOD and CAT activities while reducing the levels of ALT and MDA, thereby mitigating morphological damage in the liver. Buckwheat noodles prepared using this endogenous method were found to potentially exhibit hypoglycemic effects and mitigate complications associated with type 2 diabetes.
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2023
·
IF 2.9
Hua-Ping Zhou,Ding-Ran Wang,Chen-Lu Xu,Ye-Wang Zhang
Heparinase I (EC 4.2.2.7), is an enzyme that cleaves heparin, showing great potential for eco-friendly production of low molecular weight heparin (LMWH). However, owing to its poor catalytic activity and thermal stability, the industrial application of heparinase I has been severely hindered. To improve the catalytic activity, we proposed to engineer both the substrate and Ca2+ binding domains of heparinase I. Several heparinases I from different organisms were selected for multiple sequence alignment and molecular docking to screen the key residues in the binding domain. Nine single-point mutations were selected to enhance the catalytic activity of heparinase I. Among them, T250D was the most highly active one, whereas mutations around Ca2+ binding domain yielded two active mutants. Mutant D152S/R244K/T250D with significantly increased catalytic activity was obtained by combined mutation. The catalytic efficiency of the mutant was 118,875.8 min−1·µM−1, which was improved 5.26 times. Molecular modeling revealed that the improved activity and stability of the mutants were probably attributed to the formation of new hydrogen bonds. The highly active mutant had great potential applications in industry and the strategy could be used to improve the performance of other enzymes.
研究领域:
生物化学
·
酶工程
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DOI
PubMed
高影响因子
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