MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-9
·
IF 5.479
Zhu Dong,Hu Yue,Zhang Xiao-Jing,Yang Xiao-Tong,Tang Ying-Ying
An immunoassay is described for either colorimetric and fluorometric determination of the cancer biomarker α-fetoprotein (AFP). It is making use of ZnS nanospheres modified with CdTe quantum dots (QDs). These display strong fluorescence due to the enrichment of the QDs onto the porous ZnS nanospheres. In this assay, the release of millions of zinc(II) ions can be triggered to form a purple complex (with an absorption maximum at 571 nm) on addition of the reagent 2-(5-nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino) phenol. This results in a sensitive colorimetric immunoassay which can also be used as a visual test. It represents an enzyme-free alternative to the commonly used ELISAs. It also can be evaluated by fluorometry (with excitation/emission maxima at 400/645 nm). The detection limits are 10 pg·mL −1 for fluorometry and 7 pg·mL −1 for colorimetry. This sensitivity is better by one order of magnitude than that of the commercial ELISA. The dual detection feature provides good complementarity and reduces the risk of false-positive or false-negative results.
Schematic presentation of colorimetric and fluorescent dual-channel detection for α-fetoprotein. ZnS-CdTe hierarchical porous nanospheres are used as labels for signal amplification.
研究领域:
生物技术
·
分析化学
·
纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2014
·Vol. 30
·期 1
·60-66
·
IF 1.675
Dan Li,Shaopu Liu,Yizhong Shen,Jidong Yang,Youqiu He
Water-soluble thioglycolic acid (TGA)-capped core/shell CdTe/CdS quantum dots (QDs) were synthesized. The interactions of rhein and emodin with TGA-CdTe/CdS QDs were evaluated by fluorescence and ultraviolet-visible absorption spectroscopy. Experimental results showed that the high fluorescence intensity of TGA-CdTe/CdS QDs could be effectively quenched in the presence of rhein (or emodin) at 570 nm, which may have resulted from an electron transfer process from excited TGA-CdTe/CdS QDs to rhein (or emodin). The quenching intensity was in proportion to the concentration of both rhein and emodin in a certain range. Under optimized conditions, the linear ranges of TGA-CdTe/CdS QDs fluorescence intensity versus the concentration of rhein and emodin were 0.09650–60 µg/mL and 0.1175–70 µg/mL with a correlation coefficient of 0.9984 and 0.9965, respectively. The corresponding detection limits (3σ/S) of rhein and emodin were 28.9 and 35.2 ng/mL, respectively. This proposed method was applied to determine rhein and emodin in human urine samples successfully with remarkable advantages such as high sensitivity, short analysis time, low cost and easy operation. Based on this, a simple, rapid and highly sensitive method to determine rhein (or emodin) was proposed. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
分析化学
·
材料科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Siping Chen,Li Ji,Jinpo Xie,Chenjing Xu,Qinglan Li,Suqing Zhao
Vibrio vulnificus have seriously threatened human health through wound infection and bacterial contamination of seafood. Herein, a novel fluorescent lateral flow immunoassay (LFIA) was developed to sensitively and rapidly detect Vibrio vulnificus by using polyclonal antibodies-functionalized CdTe@SiO 2 nanocomposite (CdTe@SiO 2 -pAb) as the advanced red fluorescent signal label. The fluorescent LFIA based on CdTe@SiO 2 -pAb exhibited a wide detection range from 2.51 × 10 4 CFU/mL to 1.40 × 10 8 CFU/mL with the low limit of detection of 3.95 × 10 3 CFU/mL for Vibrio vulnificus . Moreover, the constructed LFIA displayed excellent specificity and good recovery ranging from 93.3% to 104.1% towards spiked samples. Therefore, CdTe@SiO 2 -pAb was a promising fluorescent signal label of LFIA for the sensitive and handy detection of Vibrio vulnificus in contaminated seafood.
研究领域:
生物技术
·
食品安全检测
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 7
·4981-4990
·
IF 3.201
Min Wang,Ke-yan Zheng,Shao-wu Lv,Hai-feng Zou,Hong-sen Liu,Gang-lin Yan,Ai-dong Liu,Xiao-fang Fei
Besides having many important physiological functions, cell membrane proteins have been recognized as major markers for many diseases in clinical examination. The discovery of new cell membrane proteins has attracted significant research interest. Herein, a multifunctional Fe3O4@SiO2/CdTe nanocomposite was designed for the detection and separation of cell membrane proteins, which was synthesized by a covalent coupling reaction between amino groups and carboxyl groups. It can serve as a universal tool for modifying antigens or other molecules that have amino groups. To verify the feasibility and practicability of this method, a folic acid (FA)-functionalized magnetic/fluorescent nanoprobe (Fe3O4@SiO2/CdTe-NH-FA) has been developed for recognizing and separating the FA receptor on HepG2 cells. FA was fixed to the shell of Fe3O4@SiO2/CdTe nanocomposites via 1,6-hexamethylenediamine. It obviously reduces the fluorescence quenching effect of FA on magnetic/fluorescent nanocomposites. The results demonstrate that the synthesized nanoprobe Fe3O4@SiO2/CdTe-NH-FA provides excellent optical characteristics for the reliable imaging of cancer cells and has good magnetic properties to enrich and isolate cell membrane proteins. Consequently, this biological nanoprobe can be used to find, obtain, and study unknown membrane proteins and predict the occurrence of some diseases. In addition, this nanoprobe will be a potential platform for accurate cancer diagnosis and therapy in the future.
研究领域:
纳米技术
·
生物化学
·
分子生物学
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Jing Wang,Na Li,Feng Shao,Heyou Han
Highly luminescent, good stable and low toxic N-acetyl- l -cysteine (NAC) capped CdTe/CdS@ZnS–SiO 2 near-infrared (NIR)-emitting quantum dots (QDs) were successfully fabricated in aqueous solution via a microwave irradiation reduction route, in which thiol-capped CdTe/CdS QDs were employed as core templates and ZnCl 2 , NAC and tetraethyl orthosilicate as shell precursors. This presented ZnS-like clusters filled hybrid SiO 2 model not only greatly improved the brightness and stability of original CdTe/CdS QDs, but also tremendously decreased the cytotoxicity towards HeLa cells. Furthermore, it was found that Hg 2+ could effectively selective quench the QD NIR emission based on electron transfer process. On the basis of this fact, a simple, rapid and specific method for trace Hg 2+ determination was proposed. Under optimal conditions, the fluorescence intensity decreased linearly with the concentration of Hg 2+ ranging from 5.0 × 10 −9 to 1.0 × 10 −6 M and the limit of detection for Hg 2+ was 1.0 × 10 −9 M ( S / N = 3). As practical applications, the novel NIR sensor has been demonstrated to monitor and image Hg 2+ level in milk power and HeLa cells respectively with satisfactory results obtained.
研究领域:
材料科学
·
生物医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Shenghong Yang,Jie Liu,Yu Chen,Jinxiu Guo,Lei Zhao,Xuan Wei,Xianglu Peng,Qiaosheng Pu
Rapid development of nanomaterials has extended a new horizon for sensitive and easy-to-use sensors. Robust determination of nitro-explosives with nano-sensors has attracted great attentions due to the increasing safety concern. In this work, strongly fluorescent polyacrylamide coated CdTe(S) quantum dots (CdTe(S)@PAM QDs) with good dispersity and stability have been prepared by a facile, effective and reliable photochemical method through simple one-step UV irradiation. The prepared QDs were systematically characterized and the results proved that the prepared material could be readily used for the sensitive and selective sensing of 2,4,6-trinitrotoluene (TNT). The influence of experimental conditions on the analytical performance was investigated. With a QD concentration of 3.13 μmol/L, the calibration curve of TNT was linear up to 0.48 μmol/L, and a LOD (S/N = 3) of 2.1 nmol/L was attained. In combination of simple analyte extraction, no interference was observed from common inorganic ions and organic substances. TNT in samples with complicated matrices was successfully determined. The standard addition recoveries obtained from water and soil samples were in a range of 93.2–106.3%.
研究领域:
纳米材料科学
·
环境分析化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jinyun Peng,Qing Huang,Yuxia Liu,Pingfeng Liu,Cuizhong Zhang
A novel efficient photoelectrochemical (PEC) sensor was assembled for curcumin (Cur) detection using 2-mercaptoacetic acid (TGA)-capped CdTe nanoparticles and nickel tetra-amined phthalocyanine-linked graphene oxide (TGA-CdTe@NiTAPc-Gr). The TGA-CdTe nanoparticles could be firmly immobilized on the thin film structure of NiTAPc-Gr in a hydrogen-bonded manner, thus producing the proposed PEC sensor with a high photoelectrochemical activity of TGA-CdTe nanoparticles and excellent stability of NiTAPc-Gr. The TGA-CdTe@NiTAPc-Gr thin film showed a stronger blue light absorption property than the individual precursors, and this resulted in the sensitive detection of Cur ranging from 0.25–100 μM with a detection limit of 12.50 nM. In addition, the PEC sensor exhibited a high sensitivity and selectivity and quite good reproducibility. Thus, it shows potential for Cur detection in real samples.
研究领域:
分析化学
·
生物传感
·
纳米材料
·
光电化学
·
食品安全
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 19
·2263-2271
·
IF 2.073
Yunmei Huang,Jidong Yang,Haiyan Yuan,Yuan Guo,Xiaoqing Zeng,Jiawei Cheng,Yuhui Zhang
D-Penicillamine (D-PA), as the dominant enantiomer, is usually used in many clinical drugs, but the other enantiomer, L-penicillamine (L-PA) is a toxic enantiomer. Therefore, it is of great significance to analyze and detect L-PA in biomedical or environmental samples. CdTe quantum dots (CdTe QDs) were modified by N-acetyl-L-cysteine (NALC) as novel fluorescent probes and were successfully utilized for the determination of L-penicillamine. The fluorescence of the CdTe QDs decreased significantly in the presence of cupric ions (Cu2+) due to the binding of Cu2+ to NALC on the surface of the CdTe QDs, but the addition of L-PA immediately resulted in efficient fluorescence recovery of the modulated CdTe QDs. The experimental results showed that the pH of the buffer solution and the concentration of Cu2+ affected the fluorescence intensity upon adding L-PA. Under the optimum conditions, the recovered fluorescence intensity was linearly proportional to the increasing L-PA concentration in the range of 2 × 10−7 to 4 × 10−6 mol L−1, with correlation coefficient R2 = 0.9980. So, a new, simple and rapid method for the determination of L-PA has been developed and its potential has been demonstrated in human serum samples and environmental samples with satisfactory results.
研究领域:
分析化学
·
生物医学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yanqing Ran,Rui Dai,Xiaoshuo Zhang,Faqiong Zhao,Baizhao Zeng
Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein and its detection is important for diagnosis and therapeutic prognostic assessment of many cancers. Herein, a highly sensitive electrochemiluminescence (ECL) sensor was developed for its detection. In the ECL sensor, dimercaptosuccinic acid-capped core/shell structured CdTe/CdS quantum dot (QD) was loaded on metal-organic framework material UiO-66(Ce) as electrochemical luminophore; Ti 3 C 2 /Ag composite was introduced for immobilizing EpCAM aptamer. Due to the well immobilization of QD and catalysis of UiO-66(Ce), the obtained luminophore CdTe/CdS QDs@UiO-66(Ce) displayed a strong and stable ECL signal. Furthermore, the Ti 3 C 2 /Ag could act as a co-reactant accelerator to boost the ECL signal. Thus, the resulted sensor had quite strong initial ECL signal for signal-off detection. Further experiment showed that it exhibited good linear response to EpCAM over the concentration range of 1 pg mL −1 − 10 ng mL −1 in pH 7.4 Tris buffer, with a detection limit low to 0.66 pg mL −1 . Additionally, the ECL sensor also presented high selectivity and satisfying reproducibility; its practicability was demonstrated by the successful determination of EpCAM in serum samples.
研究领域:
分析化学
·
生物化学
·
癌症研究
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2016
·Vol. 4
·期 41
·9856-9863
·
IF 5.066
Jun Yao,Xin Gou
In this paper, we report a green, ultrafast and facile aqueous preparation of highly luminescent core–shell CdTe/CdS with significant stability under atmospheric pressure. The surface topography, size, physical shape as well as crystalline structures were studied fully and in depth by adopting a variety of analytical methods such as transmission electron microscopy (TEM) and X-ray diffraction (XRD). The possible mechanism involved in the core growth was proposed and the relevant processes were conjectured too. In this study, we presented a convenient label-free fluorescence nanosensor for the rapid determination of trace mercury, which exhibited many advantages, such as a high sensitivity, excellent selectivity, a rapid response time, a wide linear range and a low limit of detection (LOD). The LOD is down to 1.7 × 10−9 mol L−1, which is lower than that of most other detection methods for mercury.
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Yali Xu,Junjie Hao,Xiaoying Niu,Shengda Qi,Hongli Chen,Kai Wang,Xingguo Chen,Tao Yi
Here we demonstrate the seed-mediated growth approach, one of the most reliable and versatile methods to control the size and shape of nanocrystals (NCs), for rapid synthesis of high performance red-emitting core/shell CdTe quantum dots (QDs) in aqueous phase. Critical parameters (including the pH value, Cd:Te molar ratio, MPA:Cd molar ratio, and the amount of CdTe seeds) affecting the growth of core/shell CdTe QDs are systematically introduced and analyzed. Under optimal conditions, the growth behaviors can be well controlled, and the high performance typically offered by the seed-mediated growth method was obtained as expected. The emission peak position quickly shifted from 540 to 680 nm in 30 min, and the red-emitting CdTe QDs thus obtained performed well, giving good size distribution, narrow full width at half maximum and high quantum yield. The growth mechanism and the resulting high structural and optical properties were also elucidated. Finally, the obtained CdTe QDs were successfully applied to highly reactive oxygen species (hROS) sensing, suggesting that this method may be useful in bioimaging and biolabeling.
研究领域:
纳米材料
·
生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2014
·
IF 3.468
Jie Sha,Cuiyan Tong,Haixia Zhang,Lijuan Feng,Bingxin Liu,Changli Lü
This paper reported a facile method for fabricating CdTe quantum dots decorated fluorescent mesoporous silica nanoparticles containing poly(p-phenylenevinylene) by electrostatic interaction in aqueous solution. The resulted fluorescent hybrid nanomaterials showed the dual-emission centered at 500 nm and 717 nm, which can be used as a fluorescent probe to detect Cu 2+ in water through fluorescence resonance energy transfer. Cu 2+ can be captured by the amino groups of the polyethyleneimine to form an absorbent complex, resulting in a strong quenching of red-emission from CdTe quantum dots on the surface of nanoparticles via energy transfer. However, the green-emission of poly(p-phenylenevinylene) remained. Therefore the addition of Cu 2+ induced the fluorescent evolution of the nanoprobe from red to green. The nanoprobe provided an efficient platform for the sensing of Cu 2+ with a detection limit of 31.2 nM. Compared with pure quantum dots, the nanoprobe can distinguish Cu 2+ from Hg 2+ .
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）