INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Benke Cui,Chunguang Ren,Renlong Zhang,Yuhang Ren,Jiaxin Liu,Yanzhe Gou,Tianyu Zhao,Xue Jiang,Hongli Cui,Xiuzhi Wang,Linlin Wang
Hypoxia can prolong the healing time of wounds and oxygen delivery to the hypoxic tissue has been reported an effective strategy for promoting wound regeneration. Bacterial infections can interfere with the wound healing process, leading to poor skin regeneration and even more serious complications, which is also an urgent issue to be solved during the process of wound healing. To address the problem of delayed wound healing caused by hypoxia and bacterial infections, we fabricated a series of Chlorella -loaded hydrogels (CK) using gelatin, κ-carrageenan and I-carrageenan as matrixes, which introduced Chlorella and ampicillin conferred oxygen-producing and antimicrobial capacity to the hydrogel. The CK hydrogels possessed good mechanical and adhesive properties, as well as the capability of efficient and sustained oxygen release. The hydrogels possessed outstanding hemostatic ability, excellent blood and cell compatibility, which also owned excellent antibacterial capacity against E. coli and S. aureus . More notably, the in vivo evaluation revealed that the hydrogel can accelerate wound healing by promoting collagen deposition, which may as a promising potential wound dressing for hypoxic wound healing.
研究领域:
生物材料学
·
医学工程
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2014
·Vol. 67
·期 4
·593-600
·
IF 1.96
Zhang Weiguo,Liu Min,Zhang Peiliang,Yu Fugen,Lu Shan,Li Pengfu,Zhou Junying
Only limited information is available on herbicide toxicity to algae under mixotrophic conditions. In the present study, we studied the effects of the herbicide paraquat on growth, photosynthetic pigments, antioxidant enzymes, and gene expression in Chlorella pyrenoidosa under mixotrophic compared with autotrophic conditions. The mean measured exposure concentrations of paraquat under mixotrophic and autotrophic conditions were in the range of 0.3–3.4 and 0.6–3.6 μM, respectively. Exposure to paraquat for 72 h under both autotrophic and mixotrophic conditions induced decreased growth and chlorophyll (Chl) content, increased superoxide dismutase and peroxidase activities, and decreased transcript abundances of three photosynthesis-related genes (light-independent protochlorophyllide reductase subunit, photosystem II protein D1, and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [ rbc L]). Compared with autotrophic conditions, the inhibition percentage of growth rate under mixotrophic conditions was lower at 0.8 μM paraquat, whereas it was greater at 1.8 and 3.4 μM paraquat. With exposure to 0.8–3.4 μM paraquat, the inhibition rates of Chl a and b content under mixotrophic conditions (43.1–52.4 % and 54.6–59.7 %, respectively) were greater compared with autotrophic conditions, whereas the inhibition rate of rbc L gene transcription under mixotrophic conditions (35.7–44.0 %) was lower. These data showed that similar to autotrophic conditions, paraquat affected the activities of antioxidant enzymes and decreased Chl synthesis and transcription of photosynthesis-related genes in C. pyrenoidosa under mixotrophic conditions, but a differential susceptibility to paraquat toxicity occurred between autotrophically versus mixotrophically grown cells.
研究领域:
环境科学
·
植物生理学
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2018
·
IF 5.807
Yun Huang,Chaoyang Wei,Qiang Liao,Ao Xia,Xun Zhu,Xianqing Zhu
To improve the carbon to nitrogen (C/N) ratio of harvested microalgae biomass for better producing biogas by fermentation, biodegradable cationic starch with high C/N ratio were synthesized to harvest Chlorella vulgaris . The impact of pH was also studied as the zeta potential of both microalgae and cationic starch would change with pH. Results indicated the cationic starch can harvest above 99% of the microalgae and the C/N ratio can rise from 7.50 to 7.90. The zeta potential of microalgae always kept negative and presented a trend of descending firstly and then upgrade. The maximum microalgae biomass flocculation capacity of 1 g cationic starch was 8.62 g with the help of self-flocculation at pH 3. The concentration of flocs formed at pH 11 was 25.74 g L −1 and the diameter was 0.553 mm which was much larger than the flocs formed at pH 3 (0.208 mm).
研究领域:
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2024
·
IF 9.7
Nuradila Zahirah Mohd Azmi,Archina Buthiyappan,Muhammad Fazly Abdul Patah,Nor Adilla Rashidi,Abdul Aziz Abdul Raman
This study aims to develop an inexpensive, environmentally-friendly adsorbent for capturing CO 2 using coconut shells, widely abundant in Asian agricultural waste. The raw coconut shell adsorbent (CS) has undergone dual functionalization utilizing Chlorella microalgae and magnesium oxide (MgO) to enhance its physicochemical properties. Through dual functionalization, minimizing the loss of surface area and reducing the temperature sensitivity often occurring during CO 2 adsorption is possible. The surface morphology , functional groups, and thermochemical properties of CS-hydrochar were characterized and evaluated. The characterization demonstrates the successful development of the ternary composite (HCS-A-Mg), with a specific surface area of 1045 m 2 /g and containing nitrogen and metal oxide functional groups. XRD analysis of HCS-A-Mg reveals crystalline peaks at 36.8°, 42.9°, and 63°, confirming the successful impregnation of MgO. The adsorption result showed that HCS-A-Mg exhibits a higher CO 2 adsorption capacity of 2.63 mmol/g, 50% higher than pristine hydrochar (HCS). The adsorption study revealed that the CO 2 adsorption capacity of HCS-A-Mg can be maximized to 3.23 mmol/g at 101 °C and 4.6 bar. Adsorption isotherms shows that the non-linear Sips model best describes the adsorption process , indicating a multilayer adsorption mechanism with profound surface heterogeneity of n = 2.3 for HCS-A-Mg, signifying higher accessibility of gas molecules deep into pores, enabling a variety of adsorption sites for CO 2 binding. Selectivity and reusability studies were conducted to validate the adsorbent applicability for industrial applications. HCS-A-Mg has higher CO 2 /N 2 selectivity by 41–57% and 0.05% loss of adsorption capacity after the 10th cyclic operation, suggesting their suitability for industrial applications. This finding underscores the potential of microalgae and magnesium oxide in advancing carbon capture technology and addressing environmental challenges.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Baoqi Ding,Susu Zhang,Wenju Jiang,Wenxin Qu,Yuan Tan,Zhiwei Ding,Weizhe Tian,Ye Yuan,Chunguang Ren,Zongliang Liu,Qun Liu
A hydrogel loaded Chlorella and berberine was constructed based on carboxymethyl chitosan, κ-carrageenan and silk fibroin. • The hydrogel exhibited excellent sustained oxygen-producing capacity. • The hydrogel demonstrated remarkable antibacterial activity and high biocompatibility. • The hydrogel can facilitate wound healing and promote tissue regeneration. Hypoxia and bacterial infection synergistically impede skin wound healing, posing significant clinical challenges. Conventional oxygen-releasing agents and antimicrobial agents are constrained by poor biocompatibility and the emergence of drug resistance. Consequently, the development of biocompatible, non-resistance-inducing oxygen-releasing antimicrobial wound dressings is of paramount importance. This study presents a κ-carrageenan (κ-Cr)/carboxymethyl chitosan (CMCS) hydrogel wound dressing loaded with Chlorella and berberine (BBR). Chlorella facilitates stable and sustained oxygen release with excellent biocompatibility, while berberine exhibits broad-spectrum antimicrobial activity without inducing drug resistance. In vitro experiments reveal that under light conditions, the hydrogel releases a cumulative 8 mg/mL of oxygen within 8 h and reduces bacterial viability to 2%, demonstrating excellent sustained oxygen-release and antimicrobial properties. The hydrogel matrix, formed via electrostatic cross-linking of κ-carrageenan and carboxymethyl chitosan, enhances adhesive performance, endowing the dressing with outstanding mechanical properties and skin adhesiveness. In vivo studies using a rat full-thickness skin wound model demonstrate the hydrogel's remarkable wound-healing efficacy: the wound healing rate reaches 98% by day 10, highlighting the hydrogel's ability to accelerate wound repair. Overall, this multifunctional hydrogel wound dressing holds substantial promise in the field of skin wound healing and provides a promising therapeutic strategy for patients with complex healing needs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Binbin Zhang,Jiarun Li,Xia Zhao,Xiuhua Hu,Lihui Yang,Ning Wang,Yantao Li,Baorong Hou
Marine corrosion and biofouling on metallic materials are sticky problems. Herein, we prepared hierarchical structured superhydrophobic (SHPB) surfaces via a versatile one step electrodeposition approach. The synthetic SHPB surface was employed as an efficient barrier against corrosion and Chlorella vulgaris -induced biofouling. The surface morphology and chemical compositions were analyzed using scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectrophotometer (FTIR) and Energy-dispersive spectrum (EDS). Electrochemical impedance spectroscopy (EIS), potentiodynamic polarization and immersion test in Chlorella vulgaris -inoculated culture medium were carried out to evaluate the anti-corrosion and anti-biofouling performance of the obtained SHPB surface. The results demonstrated that the synthetic SHPB surface exhibits great enhanced corrosion resistance and biofouling mitigation. Moreover, the as-fabricated SHPB surfaces retain superhydrophobicity in wicked environment such as strong acid and alkali conditions, showing good chemical stability. We believe that the SHPB surfaces over metallic substrates provide a potential and worthful strategy for marine corrosion and biofouling.
研究领域:
材料科学
·
海洋工程
·
生物学
✓ 已验证
DOI
高影响因子
WATER RESEARCH
·
2025
·
IF 12.4
Jiazheng Ding,Danyang Wang,Bin Ji,Anjie Li,Xiao-yan Li
Algal-bacterial granular sludge technology holds significant promise for treating municipal wastewater with carbon emission reduction. However, its practical application has been constrained by the long startup period. In this study, bio-hydrogels co-encapsulating autotrophic ammonia-oxidizing bacteria ( Nitrosomonas europaea ) and photomixotrophic microalgae ( Chlorella vulgaris ) were used in a microalgal-bacterial granules system (MBGS) for the treatment of low C/N wastewater. The operating conditions of the MBGS system included inoculation volume ratio of 1:6 (bio-hydrogel granules: wastewater), initial biomass of 0.33 g suspended solids (SS) /L, light intensity of 300 μmol/m 2 ·s, and no aeration. The results showed that this strategy reduced the typical MBGS startup period to just 6 days, with biomass accumulation (1.53 g SS/L, >4 g SS/L after running 120 days) and efficient chemical oxygen demand (COD) and total nitrogen (TN) removal (effluent COD <20.00 mg/L and TN <2.50 mg/L). Transcriptomic analysis revealed that the symbiosis with Nitrosomonas significantly upregulated the expression of the PetH gene, which encodes a reductase ( EC:1.18.1.2 ) in the photosynthetic system of Chlorella (log₂ fold change=13.63), thereby enhancing the supply of NADPH required for the Calvin cycle. Concurrently, the expression of the rbcL gene, encoding the large subunit of Rubisco in Chlorella , was upregulated by 1.4-fold, which promoted photosynthetic carbon fixation. The symbiosis also suppressed heterotrophic metabolism in Chlorella , as evidenced by downregulation of 72.5 % of genes in glycolysis and the tricarboxylic acid cycle, thereby mitigating the adverse effects of encapsulating material biodegradation on the bio-hydrogel structure. In addition, cell proliferation of Chlorella was stimulated (over 13-fold upregulation of DNA replication licensing factors MCM5 and MCM6), which increased biomass and nutrient removal efficiency. Furthermore, a higher light intensity of 400 μmol/m²/s promoted a significant increase in extracellular polymeric substances (EPS) content (106.76 mg/g SS), improved granule stability (integrity coefficient < 20 %), and enhanced biomass production (4.68 g SS/L after 120 days of operation). These findings highlight a promising strategy for sustainable wastewater treatment.
研究领域:
环境工程
·
微生物生态学
·
生物技术
·
废水处理
·
光合作用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2017
·
IF 4.208
Na Liu,Yipeng Wang,Fei Ge,Shixiang Liu,Huaixian Xiao
The interaction of nanoparticles with coexisting chemicals affects the fate and transport of nanoparticles, as well as their combined effects on aquatic organisms. Here, we evaluated the joint effect of ZnO nanoparticle (nano-ZnO) and cetyltrimethyl ammonium chloride (CTAC) on the growth of Chlorella vulgaris and explored the possible mechanism. Results showed that an antagonistic effect of nano-ZnO and CTAC (0.1, 0.2 and 0.3 mg L −1 ) was found because CTAC stop nano-ZnO being broken down into solution zinc ions (Zn 2+ ). In the presence of CTAC, the zinc (including nano-ZnO and released Zn 2+ ) showed a higher adsorption on bound extracellular polymeric substances (B-EPS) but lower accumulation in the algal cells. Moreover, we directly demonstrated that nano-ZnO was adsorbed on the algal B-EPS and entered into the algal cells by transmission electron microscope coupled with energy dispersive X-ray (TEM-EDX). Hence, these results suggested that the combined system of nano-ZnO and CTAC exhibited an antagonistic effect due to the inhibition of CTAC on dissolution of nano-ZnO and accumulation of the zinc in the algal cells.
研究领域:
环境科学
·
水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Yuxi Liu,Wenbiao Jin,Xu Zhou,Song-Fang Han,Renjie Tu,Xiaochi Feng,Paul D. Jensen,Qilin Wang
In this work, a novel flocculation process by using nano-Fe 3 O 4 coated with polyethyleneimine (Fe 3 O 4 @PEI) as magnetic seeds was developed to harvest the microalgae cultivated in urban sewage. Experiment results indicated that the harvest efficiency of Chlorella pyrenoidosa (0.5 g/L) was 98.92 ± 0.41% under the optimal conditions of Fe 3 O 4 @PEI dose of 20 mL/L, flocculation time of 20 min, and stirring speed of 800 rpm (3 min), while that of Scenedesmus obliquus (0.4 g/L) was 98.45 ± 0.35% under a Fe 3 O 4 @PEI dose of 16 mL/L, flocculation time of 15 min, and stirring speed of 730 rpm (3 min). Moreover, the process did not reduce the lipid content of microalgae and quality of biodiesel. After microalgae harvest, Fe 3 O 4 @PEI could be recovered by ultrasonication, re-wrapped with polyethyleneimine and reused to reduce operational cost.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2024
·
IF 4.6
Tong Wang,Meng Gao,Hong Song,Changhai Wang,Meilin He
Polar microalgae, renowned for their exceptional adaptability in extreme environments, are vital for carbon fixation and nutrient cycling. To understand their metabolic resilience under low temperature and high light stress, we conducted an extensive biochemical and full-length transcriptome analysis of Arctic Chlorella ( Chlorella- Arc) and temperate Chlorella ( Chlorella- Temp). This research aimed to comprehensively assess Chlorella 's adaptability to the interactive effects of temperature and light stress. Under low-temperature stress, intense light inhibited Chlorella -Temp growth by 14.8 %, while it significantly promoted Chlorella -Arc growth by 88.8 %. The cold-adapted Chlorella -Arc demonstrates higher tolerance to high light stress. Elevated temperature (+0.80) and light (+0.47) directly increased cell density, revealing direct and indirect effects of temperature and light on Chlorella- Arc bioproducts via structural equation modeling. We then analyzed key molecules involved in lipid metabolism using orthogonal projections to latent structures discriminant analysis, confirmed that Chlorella -Arc maintained a relatively constant fatty acids composition under low temperature and high light stress. Compared to its temperate counterpart, Chlorella -Arc exhibits unique adaptability by modulating polysaccharide composition, downregulating carbon fixation, reducing nitrogen absorption, and maintaining stable unsaturated fatty acid levels with a higher unsaturated to saturated fatty acid ratio. Chlorella- Arc, a prime example of polar microalgae adaptability, exhibits unique metabolic responses. Compared to the low-temperature normal-light group, the high-light Chlorella demonstrates precise regulation of nitrogen uptake and carbon allocation, while activating multiple antioxidant pathways to mitigate the increase in ROS levels under light stress. Acclimated Chlorella- Arc tends to increase triacylglycerol accumulation, suggesting a shift in carbon flow towards lipid production. Our analyses reveal specialized adjustments, enabling survival in harsh conditions of low temperature and intense light. This research enhances our understanding of microalgal resilience in extreme environments, offers valuable insights into the utilization of polar microalgal bioproduct resources.
研究领域:
藻类生物学
·
环境科学
·
代谢组学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2020
·
IF 3.905
Haohao Wu,Lu Wang,Guangbo Ji,Hanwu Lei,Hao Qu,Juan Chen,Fangbin Wang,Jian Liu
In this work, we evaluated the potential for the production of nitrogen-containing compounds (NCCs) and hydrocarbons by investigating the effects of different metal-doped catalysts (Zn/Cu/Ni/HZSM-5) on catalytic pyrolysis of chlorella using microwave heating. All bio-oils exhibited stratification with the upper/lower layer corresponding to the oil/water phase. The results showed that NCCs and hydrocarbons were the most abundant in the oil phase. The yields of these compounds were enhanced by the application of Zn/HZSM-5 and Cu/HZSM-5, reaching 55.5 area% and 47.5 area%, respectively, compared with the unmodified HZSM-5 (40.9 area%). In the water phase, the NCCs content achieved 42.3 area% with Zn/HZSM-5, 29.6 area% with Cu/HZSM-5, and 39.5 area% with Ni/HZSM-5, respectively, all higher compared with the unmodified HZSM-5 (22.4 area%). This study provides an effective method for green production of NCCs and hydrocarbons that are important structural components in many industries and promotes the application of biomass resources.
研究领域:
可再生能源
·
催化化学
·
生物质利用
✓ 已验证
DOI
高影响因子
Environmental Technology & Innovation
·
2024
·
IF 6.7
Guoming Zeng,Yu He,Xuanhao Fan,Xiaoling Lei,Quanfeng Wang,Haoxuan Wei,Da Sun
Surface modification can significantly improve nano zero-valent iron (nZVI) stability. However, different methods and materials significantly affect nZVI toxicity, and the effects on bacterial metabolic processes and corresponding molecular mechanisms require further study. In this study, Chlorella biochar-loaded nano zero-valent iron (nZVI/BC) was prepared, showing optimum removal of Microcystis aeruginosa ( M. aeruginosa ) at a dosing rate of 12.5 g L −1 . The toxicity against Escherichia coli ( E. coli ) was investigated, revealing time-dependent inactivation efficiency, achieving −3.60 log at 30 min and −5.0 log for a single nZVI. Transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) analyses revealed that nZVI exhibited stronger adsorption to cells compared to nZVI/BC, with both nZVI and nZVI/BC primarily interacting with amine, carboxyl, and ester groups on the cell surface. Physiological, biochemical, ROS quenching, and metabolomics experiments indicated that ROS played a key role in the inactivation of E. coli . This study proposes a strategy to balance the stability of nZVI with its biocompatibility.
研究领域:
环境科学
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）