Food Bioscience
·
2023
·
IF 5.2
Hengju Ge,Jiaxin Li,Yang Xu,Jiahong Xie,Naymul Karim,Fujie Yan,Jianling Mo,Wei Chen
Curcumin (CUR), the main compound extracted from Curcuma longa , has various biological actions, including hepatoprotective, anti-infective, anti-inflammatory, antioxidant, and neurotrophic properties. But it has not yet been clarified how CUR affects the accumulation of fatty in hepatic cells. Our study uncovered that CUR decreased the lipid deposition in free fat acids (FFAs)-treated hepatocytes, which could be blocked by Compound C (an AMPK inhibitor). This result suggests that protective effect was associated with the AMPK pathway activation. MiR-3666 directly targets the AMPK gene, and overexpression of miR-3666 greatly weakens the hypolipidemic effect of CUR. Our results showed that CUR reduced FFAs-induced lipid deposition by targeting miR-3666. This boosted AMPK phosphorylation and inhibited its downstream targets, including ACC and SREBP1 . These findings together confirm that CUR-mediated inhibition of miR-3666 to activate the AMPK pathway is a potential alternative for alleviation of hepatic lipid deposition.
研究领域:
药理学
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分子生物学
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肝病学
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产品关联
DOI
高影响因子
MATERIALS & DESIGN
·
2022
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IF 9.417
Liangjun Zhang,Haihan Xia,Song Du,Nan Zhao,Xiaoxun Zhang,Qiong Pan,Senlin Xu,Zhicheng He,Zeng Yi,Jin Chai
Hyperinflammation can lead to tissue injury, organ failure, and even fatalities by overproducing proinflammatory cytokines. The long-term safety and outstanding anti-inflammatory ability of natural products have attracted much attention, but the low solubility and bioavailability limit their application. In this study, PGDE was conjugated with aromatic hydroxy groups of curcumin, resveratrol, and quercetin to prepare polyphenolic nano-assemblies. The nano-assemblies showed a comparable capacity for scavenging DPPH and ABTS free radicals with curcumin. The modification not only decreased the cytotoxicity of curcumin but also the anti-inflammation activity was preserved successfully. In vivo tests, NCAs were preferentially located at the inflammatory sites and performed effective therapeutic effects on acute and chronic inflammation models including sepsis, IBD, and liver fibrosis models. The anti-inflammatory activities were primarily realized by suppressing the overproduction of TNF-α, IL-6 and IL-β cytokines, and down-regulating the ROS-related NOX-1 and NOX-4. Additionally, NCAs inhibited the activation of the TGF-α/Smad pathway and decreased the production of α-SMA to alleviate collagen deposition in fibrosis mice. This study described a simple method for preparing nano-assemblies of insoluble polyphenols via the carrier-free self-assembly method, which improved the therapeutic effect on inflammatory diseases and broadened the application field of insoluble natural active polyphenols.
研究领域:
药理学
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生物医学工程
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免疫学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2022
·
IF 7.033
Jiangxiu Niu,Ming Yuan,Zhaowei Zhang,Liye Wang,Yanli Fan,Xianghui Liu,Xianming Liu,Huiyuan Ya,Yansong Zhang,Yang Xu
Background The poor skin permeation and deposition of topical therapeutic drugs is a major issue in topical drug delivery, improving this issue is conducive to improving the topical therapeutic effect of drugs. Methods In this study, octadecylamine modified hyaluronic acid (OHA) copolymer was synthesized by amide reaction technique to prepare curcumin (CUR)-loaded micelles (CUR-M) for topical transdermal administration. CUR-M was successfully prepared by dialysis, and the formulation was evaluated for particle size, zeta potential, surface morphology, entrapment effciency (EE%), drug loading (DL), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and the in vitro drug release. Additionally, in vitro skin permeation and retention, in vivo topical analgesic and anti-inflammatory activity, and skin irritation were assessed. Results The mean drug loading (DL), drug entrapment efficiency (EE), hydrodynamic diameter and zeta potential of CUR-M were 8.26%, 90.86%, 165.64 nm and −26.85 mV, respectively. CUR-M was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), it was found that there was an interaction between CUR and OHA, and CUR existed in CUR-M in an amorphous form. CUR-M exhibited sustained release in 48 h and good stability at 4 °C for 21days. CUR-M could significantly increase the skin penetration and retention of CUR and had better analgesic and anti-inflammatory activities in vivo when compared with CUR solution. Hematoxylin-eosin staining results revealed that the transdermal penetration mechanism of CUR-M might be related to the hydration of stratum corneum by HA. In addition, CUR-M showed no skin irritation to mouse skin. Conclusion CUR-M might be a promising and safe drug delivery system for the treatment of topical diseases.
研究领域:
药剂学
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皮肤科学
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生物材料
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Guangyi Kan,Ye Zi,Li Li,Huan Gong,Jiawei Peng,Xichang Wang,Jian Zhong
Herein, pH-cycle method was explored to prepare curcumin-encapsulated hydrophilic bovine bone gelatin (BBG/Cur) nanoparticle and then the obtained nanoparticle was applied to stabilize fish oil-loaded Pickering emulsion. The nanoparticle had a high encapsulation efficiency (93.9 ± 0.5 %) and loading capacity (9.4 ± 0.1 %) for curcumin. The nanoparticle-stabilized emulsion had higher emulsifying activity index (25.1 ± 0.9 m 2 /g) and lower emulsifying stability index (161.5 ± 18.8 min) than BBG-stabilized emulsion. The pH affected the initial droplet sizes and creaming index values of the Pickering emulsions: pH 11.0 < pH 5.0 ≈ pH 7.0 ≈ pH 9.0 < pH 3.0. Curcumin provided obvious antioxidant effect for the emulsions, which was also dependent on pH. The work suggested pH-cycle method could be used to prepare hydrophobic antioxidant-encapsulated hydrophilic protein nanoparticle. It also provided basic information on the development of protein nanoparticles for Pickering emulsion stabilization.
研究领域:
食品科学
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纳米技术
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材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2023
·
IF 8.4
Jingfei Wang,Qingye Meng,Ying Ma,Yongyan Yang,Ruiting Zhang,Shuangling Zhong,Jia Wang,Yan Gao,Xuejun Cui
As outstanding natural materials, polysaccharide materials have attracted extensive research attention in the field of biomedical materials due to their nontoxicity, biocompatibility, and biodegradability. In this study, carboxymethylcellulose (CMC) was used to develop a wound dressing hydrogel (RSMCs@CUR/CMC hydrogels) based on photo-cross-linking and a microcapsule platform (RSMCs@CUR) with curcumin to achieve quick healing of skin wounds. The combination of RSMCs@CUR and CMC hydrogels can significantly improve the mechanical properties, stability, and antibacterial properties of hydrogels. RSMCs@CUR/CMC hydrogels can possess a compressive strength of 5.3 MPa and a tensile strength of 2.5 MPa. Compared to CMC hydrogels, antibacterial properties were significantly improved. Thus, RSMCs@CUR/CMC hydrogels provided continuous drug release in different pH environments, which can suit different periods during the wound healing process. The accumulated drug release percentage of a hydrogel can reach 24.37% in a phosphate-buffered solution (PBS). Hydrogels also exhibited good biocompatibility. The cell viability remained above 90% when cultured with NIH 3T3 cells. The wound dressing hydrogel also reduced inflammation and prevented wound infection. The RSMCs@CUR/CMC hydrogel can significantly improve the wound healing rate; the wound contraction rate was about 98.9% on the 14th day.
研究领域:
生物医学材料
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伤口护理
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药学科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Lan Wang,Jin Mao,Qi Zhou,Qianchun Deng,Lei Zheng,Jie Shi
The food industry has paid lots of attentions to curcumin because of its potential bioactive qualities. However, its use is severely constrained by its low bioavailability, stability and water solubility. Herein, we created sodium caseinate and carboxymethylpachymaran (CMP) nanoparticles (SMCNPs) that were loaded with curcumin. The composite nanoparticles were spherical, as characterized by SEM and TEM , the fluorescence spectroscopy, FTIR and XRD research revealed that hydrogen bonding, hydrophobic interaction and electrostatic interaction were the main drivers behind the creation of the nanoparticles. The SMCNPs exhibited lower particle size, greater dispersion and higher encapsulation rate when the mass ratio of sodium caseinate to CMP was 3:5 (particle size of 166.8 nm, PDI of 0.15, and encapsulation efficiency of 88.07 %). The composite nanoparticles had good antioxidant activity , physical stability and sustained release effect on intestinal tract during the in vitro simulation experiments, successfully preventing the early release of curcumin into gastric fluid. Finally, cytotoxicity studies told that the prepared composite nanoparticles have good biocompatibility and can inhibit the growth of tumor cells (HT-29). In conclusion, using CMP and sodium caseinate as carriers in this study may open up a fresh, environmentally friendly, and long-lasting way to construct a bioactive material delivery system.
研究领域:
食品科学
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药物递送系统
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2019
·Vol. 10
·期 20
·2551-2558
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IF 4.76
Wenyu Cheng,Hongtao Chen,Chendong Ji,Ru Yang,Meizhen Yin
Perylenediimide-cored (PDI-cored) polymers have been widely applied as fluorescent drug carriers due to their high fluorescence quantum yield and outstanding chemical/photophysical stability. However, most of these delivery systems lack detailed drug delivery specifics, especially when and where the drug is released. In this study, a PDI-based nanocarrier (PPL-B) is constructed and applied to monitor curcumin release with an “off–on” fluorescence switch. The star-shaped drug carrier has an amphiphilic structure with fluorescent PDI as the core and poly(amino acid) as the shell, which is suitable for drug delivery. A model drug curcumin is loaded to PPL-B and quenches its fluorescence significantly through photo-induced electron transfer (PET). Upon curcumin release, the fluorescence of PPL-B achieves an “off–on” switch as a result of PET interruption. In light of this, curcumin release is successfully monitored by CUR@PPL-B at the cellular level. This PDI-based nanocarrier for drug release monitoring with a PET mechanism shows great potential in the drug delivery field.
研究领域:
药学
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材料科学
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生物医学工程
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纳米技术
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2015
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IF 2.818
Yujia Liu,Dandan Liu,Li Zhu,Qian Gan,Xueyi Le
Liposome, a promising delivery system with phospholipid bilayer, could improve the water solubility of curcumin, which is a polyphenolic natural product with many bioactivities. However, the instability of liposome limits its development in application. In this study, the chitosan was used to protect the structure stability of phospholipid bilayer of curcumin liposome and control the sustained release rate of curcumin further. The temperature stability and phase transition were studied by differential scanning calorimetry. When curcumin molecule was embedded in phospholipid bilayer, the pre-transition temperature (50.40 °C) and main phase transition temperature (61.47 °C) decreased about 10 °C compared to it without curcumin, and phospholipid bilayer fluidity was increased. The pre-transition temperature was disappeared after chitosan coated on the surface of curcumin liposome. The thermal stability test and X-ray diffraction also indicate that high temperature could change the structure of liposome and chitosan could protect the curcumin from damage and leak. The in vitro release study showed that the cumulative release rate got faster with temperature increased and chitosan could decrease the release rate. These results may guide the potential application of chitosan-coated liposome as a carrier of curcumin in nutraceutical and functional foods.
研究领域:
药剂学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
·
2026
·
IF 3.2
Xi Su,Wei Xu,Lulu Liu,Qingxiang You,Wenchang Yi,Siyu Chen,Zhenying Wan,Shuya Huang
A novel ethyl cellulose-sodium alginate/chitosan/curcumin (EC-SA/CS/Cur) composite microgel system was developed through a one-step emulsion method. Optimized homogenization at 1,300 rpm for 2 min enabled consistent fabrication of EC-SA/CS/Cur microgels with a mean particle size of 2.85 μm. The microstructural morphology, particle size distribution, and chemical composition of the optimized EC-SA/CS/Cur microgels were characterized by microscopy, laser particle size analysis, and Fourier-transform infrared spectroscopy. The microgels exhibited a curcumin encapsulation efficiency of 54.4 ± 6.8% and a drug loading capacity of 0.032 ± 0.004%. The microgels exhibited excellent stability under enzymatic and physiological pH environments while displaying a unique sustained-release profile in ethanol-containing media; under agitated conditions, a cumulative release of 3.2 μg/mL was achieved within 6 h, representing a 28.0% increase in cumulative release compared to static conditions. The minimum inhibitory concentration of EC-SA/CS/Cur microgels was determined as 675 μg/mL; after 3 h of exposure, their antimicrobial efficacy significantly outperformed free curcumin. These results confirm that the EC-SA/CS microgel system acts as an effective delivery vehicle, markedly enhancing curcumin’s antimicrobial performance. This study provides a promising strategy for developing stable, high-efficiency curcumin delivery systems with improved therapeutic potential, particularly for oral/throat protection against alcohol-induced irritation via on-site curcumin release to alleviate local inflammatory damage.
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产品关联
DOI
高影响因子
CELLULOSE
·
2020
·Vol. 27
·期 17
·10541-10551
·
IF 4.21
Wang Mingyue,Liu Mao,Zhao Hongjuan,Xiong Xiaoqing,Zheng Laijiu
Abstract Modified curcumin, which has the advantages of low toxicity, high solubility, and little color change after modification, is very suitable for nonaqueous supercritical carbon dioxide cotton dyeing. However, the existing curcumin modification technology focuses on improving its solubility. Modified curcumin is directly adsorbed onto cotton fabrics, and the main interaction forces are van der Waals forces, which causes the poor fastness of modified curcumin to dyed cotton fabrics. In this study, reactive groups (hydroxyalkyls) were introduced into curcumin to enhance its fastness to cotton fabrics. The results showed that, similar to alkyl groups, hydroxyalkyl groups can enhance the color depth of dyed cotton fabrics by elevating the solubility of modified curcumin in supercritical carbon dioxide. The higher the solubility of modified curcumin in supercritical carbon dioxide was, the higher the K/S value of the dyed cotton fabric. Furthermore, the hydroxyl groups on the modified curcumin could form ether bonds with the hydroxyl groups on cotton fabrics. Therefore, the washing and rubbing fastness of hydroxyalkyl-curcumin-dyed cotton fabric was better than that of alkyl-curcumin-dyed cotton fabric. Graphic abstract
研究领域:
染料化学
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纺织科学
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材料科学
✓ 已验证
DOI
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2020
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IF 3.343
Baiji Xue,Jinxiang Huang,Haoxiang Zhang,Bingliang Li,Man Xu,Yuelan Zhang,Maobin Xie,Xuefeng Li
Curcumin (CM) is a natural polyphenolic compound with multiple biomedical functions. However, clinical applications face more challenges due to its low dissolution rate and poor bioavailability. Micronization is an effective strategy to overcome these drawbacks. Herein, CM nanoparticles (CM NPs, ∼300 nm) were fabricated using solution enhanced dispersion by supercritical CO2 (SEDS). The solubility of CM NPs was remarkably enhanced. Aim to study the effects of micronization on the biological functions of CM, we investigated the antibacterial activity of original CM and CM NPs upon Pseudomonas aeruginosa. In vitro, the minimal inhibitory concentrations (MIC) assay, solid-medium spot assay, growth kinetics assay and morphologic observation using atomic force microscopy (AFM) confirmed that the anti-P. aeruginosa activity of CM NPs was enhanced compared to original CM. Moreover, CM NPs also showed stronger inhibition for adhesion and biofilm formation of P. aeruginosa compared to original CM. Experiments on mice infected with P. aeruginosa showed that CM NPs have a better therapeutic effect than the original CM in vivo. In summary, CM NPs may be a novel and promising therapeutic candidate for bacterial infection.
研究领域:
药剂学
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纳米技术
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微生物学
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生物医学工程
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药理学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Bingchao Cheng,Hao Pan,Dandan Liu,Dongyang Li,Jinyu Li,Shihui Yu,Guoxin Tan,Weisan Pan
The purpose of this work was to develop a d -α-tocopherol polyethylene glycol 1000 succinate (TPGS) decorated nanodiamond (ND) system loading water-insoluble curcumin (ND/CUR/TPGS) to improve the colloidal dispersity and oral bioavailability of the preparation. CUR was physically loaded into ND clusters, then TPGS was coated to the ND/CUR complex forming amorphous nanostructure on the interparticle nanocage of the ND substrate. The formulation of the nanocomplexes was optimized using response surface methodology, and the optimal ND/CUR/TPGS showed small particle size (196.32 nm), high drug loading efficiency (81.59%) and core-shell structure. In vitro release study demonstrated that the nanocomplexes provided a sustained release behavior. The absorptive concentration of ND/CUR/TPGS was dramatically improved in total intestinal tract compared with CUR suspension, and the absorption was controlled by multiple transcytosis mechanisms. Furthermore, the pharmacokinetic studies demonstrated that ND/CUR/TPGS had significantly higher C max (4.50-fold), larger AUC 0–t (10.67-fold), and longer MRT 0–t (3.07-fold) in contrast with that of CUR suspension. Therefore, ND/CUR/TPGS presented great potential for oral delivery of insoluble and poorly permeable drugs.
研究领域:
药剂学
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纳米技术
·
药物递送系统
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）