Frontiers in Pharmacology
·
2018
·
IF 3.831
Mao Jingxin,Yi Man,Wang Rui,Huang Yuanshe,Chen Min
Costunolide, a sesquiterpene isolated from Vladimiria souliei (Franch.) Ling, is known to exhibit anti-inflammatory, anti-viral, and anti-tumor activities. However, the effects of costunolide on liver injury are poorly understood. The current study aimed to investigate the hepatoprotective effects of costunolide against lipopolysaccharide (LPS) and D-galactosamine-induced acute liver injury (ALI) in mice. The results indicated that costunolide (40 mg/kg) could significantly improve the pathological changes of hepatic tissue, and reduced the LPS and D-galactosamine-induced increases of alanine aminotransferase (from 887.24 ± 21.72 to 121.67 ± 6.56 IU/L) and aspartate aminotransferase (from 891.01 ± 45.24 to 199.94 ± 11.53 IU/L) activities in serum. Further research indicated that costunolide significantly reduced malondialdehyde content (from 24.56 ± 1.39 to 9.17 ± 0.25 nmol/ml) and reactive oxygen species (from 203.34 ± 7.68 to 144.23 ± 7.12%), increased the activity of anti-oxidant enzymes superoxide dismutase (from 153.74 ± 10.33 to 262.27 ± 8.39 U/ml), catalase (from 6.12 ± 0.30 to 12.44 ± 0.57 U/ml), and total anti-oxidant capacity (from 0.64 ± 0.06 to 6.29 ± 0.11 U/ml) in hepatic tissues. Western blot results revealed that costunolide may trigger the anti-oxidative defense system by inhibiting kelch-like ECH-associated protein 1 and nuclear factor-related factor 2 (cytosol), increasing nuclear factor-related factor 2 (nucleus), heme oxygenase-1 and NAD (P) H quinone oxidoreductase 1 activity. Moreover, costunolide significantly decreased the protein expression of proinflammatory cytokines including interleukin 1β, interleukin 6, and tumor necrosis factor. Pretreatment with costunolide could reduce the expression of toll-like receptor 4, myeloid differentiation factor 88, p65 (Nucleus), phosphorylated IκB kinase α/β, inhibitor of nuclear factor kappa-B kinase, inhibitor kappa Bα and prevent the expression of phosphorylated inhibitor kappa B kinase which repressed translocation of p65 from cytoplasm to nucleus. In addition, pretreatment with costunolide also inhibited hepatocyte apoptosis by reducing the expression of B-cell lymphoma 2 associated X, cytochrome C, cysteinyl aspartate specific proteinase 3, cysteinyl aspartate specific proteinase 8 and cysteinyl aspartate specific proteinase 9, and by increasing B-cell lymphoma 2. From the above analysis, the protective effects of costunolide against LPS and D-galactosamine-induced ALI in mice may be attributed to its anti-oxidative activity in nuclear factor-related factor 2 signaling pathways, anti-inflammatory suppression in nuclear factor-kappa B signaling pathways, and inhibition of hepatocyte apoptosis. Thus, costunolide may be a potential therapeutic agent in attenuating LPS and D-galactosamine -induced ALI in the future.
研究领域:
药理学
·
肝病学
·
免疫学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2017
·
IF 1.795
Wen Zhang,Qi Tang,Yan-Fang Feng,Kai-Liang Luo,Zhong Zhang,Yu-Ning Liang
Four new d-block metal complexes formulated as [NiII(H3thptacn)]Cl2·H2O (1), [MnIV(thptacn)]ClO4 (2), [CuII3(Hthptacn)2](ClO4)2 (3), and [CdII2(H3thptacn)2Cl2][B(C6H5)4]2 (4) were obtained from the macrocyclic ligand 1,4,7-tris(3-hydroxypropyl)-1,4,7-triazacyclononane (H3thptacn) either through solvent diffusion or by evaporation of their solutions. These complexes were characterized by single crystal X-ray structural determination, elemental analysis, and routine spectroscopic methods. Complexes 1 and 2 exhibit similar mononuclear structures with the metal centers being surrounded by both the backbone nitrogen atoms and the pendant coordinating alcohol/alkoxide groups. Complex 3 is a linear trinuclear cluster, where three Cu(II) centers are combined together by the bridging alkoxide groups in a centro-symmetric pattern. Two chloride groups join two Cd(II) atoms each chelated by one triply protonated ligand H3thptacn to afford dinuclear compound 4 with a symmetry center. When coordinating to different d-block metals, the macrocyclic ligand exhibits four types of binding modes with various dissociation status on its pendant alcohol groups and different numbers of these pendant groups participating in coordination. The magnetic measurements revealed significant zero-field splitting for mononuclear Ni(II) and Mn(IV) complexes. A moderate antiferromagnetic interaction between the neighboring Cu(II) centers governs the magnetic properties of 3 with J = −166(3) cm−1.
研究领域:
化学
·
材料科学
·
配位化学
✓ 已验证
DOI
高影响因子
Journal of Energy Chemistry
·
2025
·
IF 14.9
Xiaona Zhang,Zeyang Liu,Wenxuan Guo,Wei An,Zhaoyi Song,Yizhang Du,Tongjun Shen,Qihao Qin,Chunxia Wang,Guoyong Huang,Shengming Xu,Junjie Mao
The strong hydrogen binding affinity on Ru surfaces and their intrinsic aggregation tendency pose significant challenges to the hydrogen evolution reaction (HER) activity of Ru-based electrocatalysts. The construction of active electrocatalysts composed of partially dispersed nanoparticles (NPs) and individual single atomic sites with robust thermodynamic stability, has emerged as a viable alternative to benchmark commercial HER electrocatalyst. Herein, a multi-step strategy was designed to synthesize Ru NP @Fe SA -NC electrocatalyst and a robust interaction between uniformly dispersed Ru NPs and embedded single-atom Fe sites was uncovered, which not only regulates the particle size of Ru NPs but also controls the spin state and electronic configuration of Fe single atom. Moreover, magnetic characterization further corroborates the synergetic effect and predicts that the synergetic effect induces a high spin state of the Fe atom with unpaired electrons in the 3 d orbitals, which enhances the adsorption of intermediates and accelerates the reaction kinetics. The as obtained electrocatalyst demonstrate exceptional performance with a low overpotential of 13 mV at 10 mA cm -2 in alkaline condition. Remarkably, theoretical calculations indicates that the outstanding performance of Ru NP @Fe SA -NC stems from the Fe optimized electronic structure of the Ru site, which downshifts the d-band center, reduces the energy barriers for water dissociation and optimizes H* desorption, thereby promoting HER. This study presents an innovative approach to utilize Fe SA -NC to stabilize Ru NPs and reduce the energy barrier, contributing to an ideal HER performance.
研究领域:
材料科学
·
电化学
·
理论计算
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Shuju Zhao,Nianzu Liu,Wenqi Wang,Zhenying Xu,Yumin Wu,Xiliang Luo
The development of antifouling biosensors capable of detecting biomarkers at low concentrations in complex bio-fluids with many interference components is of great importance in the diagnosis and treatment of diseases. Certain zwitterionic peptides composed of natural L-amino acids have been used for the construction of low fouling biosensors and demonstrated excellent antifouling performances, but they are prone to enzymatic degradation in biological media, such as serum that contains a variety of enzymes . In this work, a novel antifouling peptide with the sequence of cppPPEKEKEkek was designed, and three unnatural D-amino acids were set at both ends of the peptide to enhance its tolerance to enzymatic degradation. An electrochemical biosensor was constructed by coupling the antifouling peptide with a conducting polymer polyaniline (PANI) to achieve accurate detection of alpha-fetoprotein (AFP) in clinical samples. Owing to the presence of the designed peptide with partial D-amino acids (pD-peptide), the biosensing interface showed significantly high antifouling performance and enhanced stability in human serum. Meanwhile, the pD-peptide based biosensor exhibited high sensitivity toward the target AFP, with the linear range from 0.1 fg mL −1 to 1.0 ng mL −1 and the limit of detection of 0.03 fg mL −1 (S/N = 3). This strategy of enhancing the stability (tolerance to enzymolysis) of antifouling peptides in biological samples provided an effective way to develop antifouling biosensors for practical applications.
研究领域:
生物传感器
·
分析化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2025
·
IF 6.4
Ruichen Geng,Lin Su,Xiaojun Chu,Zhihan Zhang,Ranran Wei,Yinglong Wang,Shuli Yin
Developing electrocatalysts with high activity and selectivity to promote the oxidation of 5-hydroxymethylfurfural (HMF) holds substantial importance yet presents challenges in the realm of biomass upgrading. Herein, the defect-rich high-entropy alloy AuCuAgRuNi nanofibers (AuCuAgRuNi NFs) were synthesized via a facile one-pot wet chemical synthesis. Remarkably, the AuCuAgRuNi NFs demonstrate exceptional electrocatalytic performance for HMF-to-FDCA conversion under ambient conditions, achieving 97.15% yield and 98.40% selectivity with outstanding stability. Theoretical calculations reveal that defect-induced electronic modulation optimizes reactant adsorption, while d-orbital hybridization among multi-metallic components facilitates electron redistribution, synergistically enhancing catalytic activity. Furthermore, HMF oxidation has been successfully integrated with hydrogen evolution in a flow electrolysis cell, and an energy-efficient coupled system has been established, which holds promise for scalable biomass valorization. This research provides valuable insights into the innovative synthesis and inherent catalytic mechanisms of high-entropy alloy systems.
研究领域:
催化化学
·
纳米技术
·
电化学
·
材料科学
·
生物质转化
·
理论化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2023
·
IF 22.1
Fengwei Huang,Xiaoyun Dong,Yuexin Wang,Xianjun Lang
Conjugated microporous polymers (CMPs) have evloved into versatile visible light photocatalysts. Narrowing the bandgaps of CMPs could capture more visible light without shifting the suitable position with an established electron acceptor like thiazolo[5,4- d ]thiazole (TzTz). Here, benzene-1,3,5-tricarbaldehyde (Bz-CHO) and benzo[1,2-b:3,4-b':5,6-b'']trithiophene-2,5,8-tricarbaldehyde (BTT-CHO) are adopted to undergo condensation with dithiooxamide, preparing two TzTz-bridged CMPs, namely TzTz-Bz and TzTz-BTT. Thereby, TzTz-BTT could capture visible light more substantially than TzTz-Bz due to narrowing the bandgap. Endowed by TzTz, both TzTz-BTT and TzTz-Bz possess similar positions for the activation of O 2 and can drive the subsequent formation of imines. However, TzTz-BTT, with a narrower bandgap, could capture more green light to drive the electron and proton transfer for the selective formation of imines with higher conversions than TzTz-Bz. This work suggests that CMPs can be equipped with extensive visible light absorption for photocatalytic selective oxidations.
研究领域:
材料科学
·
光催化
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Yuhan Zheng,Feiyang Sun,Siyi Liu,Gang Wang,Huan Chen,Yongxin Guo,Xiufeng Wang,Maia Lia Escobar Bonora,Sitong Zhang,Yanli Li,Guang Chen
Background: The production of D-lactic acid (D-LA) from non-detoxified corn stover hydrolysate is hindered by substrate-mediated inhibition and low cell utilization times. In this study, we developed a novel temperature-sensitive hydrogel, F127-IEA, for efficient D-LA production using a cell-recycle batch fermentation process.Results: F127-IEA exhibited a porous structure with an average pore size of approximately 1 μm, facilitating the formation of stable Lactobacillus bulgaricus clusters within the gel matrix. It also maintains excellent mechanical properties. It also maintains excellent mechanical properties. F127-IEA immobilized Lactobacillus bulgaricus T15 (F127-IEA-T15) can be used in cell-recycle fermentation for over 150 days from glucose and 50 days from corn stover hydrolysate, achieving high production rates of D-LA from glucose (2.71 ± 0.85 g/L h) and corn stover hydrolysate (1.29 ± 0.39 g/L h). F127-IEA-T15 enhanced D-LA production by adsorbing and blocking toxic substances present in corn stover hydrolysate that are detrimental to cellular activity.Conclusions: The newly developed hydrogels in this study provide a robust platform for large-scale extraction of D-LA from non-detoxified corn stover.
研究领域:
微生物学
·
生物工程
·
发酵工程
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Lili Liu,Peng Bao,Juan Qiao,Hongyi Zhang,Li Qi
Herein, D- and L-oligopeptides were explored for the first time as chiral ligands in a chiral ligand exchange capillary electrophoresis (CLE-CE) protocol with Zn(II) as central ion for separation of derived d , l -amino acid enantiomers (Dns-D,L-AAs). The effect of four D- and L-oligopeptides, including glycine- l -lysine (Gly-L-Lys), glycine- d -lysine (Gly-D-Lys), l -lysine-lysine-OH and l -lysine-lysine-lysine-lysine-OH on the CLE-CE separation efficiency were evaluated. Thermodynamic calculations and circular dichroism spectra properties showed that the ternary species [(Gly-L-Lys)Zn(II)(D-AAs)] and [(Gly-L-Lys)Zn(II)(L-AAs)] presented the best stereoselectivity , possibly due to entropic effects. Notably, the migration order of Dns-D-AAs and Dns-L-AAs could be tuned by using Gly-D-Lys as the ligand for the CLE-CE system. To obtain satisfactory CLE-CE performance, the concentration ratio of Zn(II) to Gly-L-Lys, the pH of the buffer solution, and the concentration of Zn(II) were investigated. Under the optimized CLE-CE conditions using 100.0 mM H 3 BO 3 , 10.0 mM NH 4 Ac, 3.0 mM Zn (II) and 12.0 mM Gly-L-Lys as the buffer solution at pH 8.30, nine pairs of Dns-D,L-AAs achieved baseline separation, with the partial separation of another five pairs. Furthermore, the proposed CLE-CE protocol, which presented a good linear relationship with the concentration of the test analytes in the range of 75.0–625.0 μM (r 2 ≥ 0.994) and the limit of detection of the method was 5.0 μM, was successfully applied in the kinetics study of l -asparaginase using l -asparagine as the substrate. Our strategy shows the great potential of L-dipeptides in the CLE-CE separation of D,L-AAs enantiomers and bio-applications.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2018
·
IF 0.827
Liwei Cao,Tian Wei,Yihui Shi,Xiaofang Tan,Jianxin Meng
Two rapid and sensitive analytical methods are developed for the determination of D-penicillamine (D-PEN) and tiopronin (TP) through high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). A boron-dipyrrolemethene (BODIPY) fluorescence labeling reagent, 1,3,5,7-tetramethyl-8-bromomethyl-difluoroboradiaza-s-indacene (TMMB-Br) was successfully applied to derivatize these two thiol drugs. Fluorescein was used as the internal standard (IS) for the quantification of D-PEN and TP in CE-LIF. The derivatization and separation conditions were optimized carefully. Under the optimum conditions, the HPLC and CE separation of D-PEN and TP could be achieved within 12 min. The limits of detection were as low as 2.0 nmol/L for HPLC-FLD and 0.47 nmol/L for CE-LIF. The drugs in human urine and serum samples were determined successfully, and the recoveries were 95.0–06.7% and 95.2–104.3%, respectively.
研究领域:
分析化学
·
药物分析
·
生物医学
✓ 已验证
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 3.7
Yun Liu,Ao Huang,Xiaowei Wen
Phospholipase D (PLD) is essential for the bioconversion of phosphatidylcholine (PC) to phosphatidylserine (PS), a process valuable in functional food and medicine. This study explores the stability and catalytic properties of PLD immobilized on chitosan-encapsulated magnetic nanoparticles (CMNPs), utilizing oxidized dextran (DX) and glutaraldehyde (Glu) as cross-linkers. The cross-linker concentration and immobilization time were optimized to assess their effects on PLD catalytic performance. PLD immobilized on CMNPs with DX (DX-CMNPs-PLD) exhibited optimal activity at pH 8.0 and 30 °C, retaining over 40 % activity after 14 cycles, while Glu-cross-linked PLD (Glu-CMNPs-PLD) retained approximately 65 %. DX-CMNPs-PLD demonstrated superior pH, temperature, and operational stability compared to free PLD. Additionally, the immobilized PLD was characterized using transmission electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. Kinetics parameters (V max and K m ) of the immobilized PLD were also studied with free PLD serving as a control. Conformational analyses indicated a significant change in PLD's secondary structure, particularly in β-sheet content, which likely contributed to the enhanced stability and activity. These findings suggest a promising approach for PLD immobilization on CMNPs, with notable implications for biotechnological applications.
研究领域:
生物技术
·
食品科学
·
医学
✓ 已验证
DOI
高影响因子
FOOD MICROBIOLOGY
·
2022
·
IF 6.374
Jian Chen,Rendi Yang,Yongzheng Wang,Shigenobu Koseki,Linglin Fu,Yanbo Wang
In this study, we found the inhibitory effect of d -Tryptophan ( d -Trp) on the bacterial growth and spoilage potential of P. fluorescens and S. baltica . The effectiveness of d -Trp in bacterial growth inhibition was highly dependent on environmental NaCl concentrations. The growth/no growth models were developed to clarify the relationship between salt and d -Trp concentrations for optimal growth-inhibitory effect. It showed that higher levels of NaCl (>3.5%) with d -Trp (>15 mM) are required for growth inhibition. The d -Trp treatment combined with NaCl also retarded the psychrotrophic growth and reduced the respiratory active bacteria at low temperature, indicating that the inhibitory effect of d -Trp might be associated with the tolerance to osmotic and cold stress. Furthermore, the treatment d -Trp combined NaCl (artificial seawater) on salmon fillets was investigated. During refrigerated storage, the d -Trp treated salmon fillet either inoculated with S. baltica or P. fluorescen maintained remained a relatively low level of total volatile basic nitrogen (TVBN) (below 30 mg N/100 g during 8-days storage) and delayed increase of low total viable bacteria counts (TVC) with a longer lag phase of 2 days.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Ruoqing Zeng,Meimiao Fang,Ancheng Shen,Xiaolei Chai,Yumiao Zhao,Mingyao Liu,Lingfeng Zhu,Weiwei Rui,Bo Feng,Liang Hong,Chunyong Ding,Zilan Song,Weiqiang Lu,Ao Zhang
Accumulating evidence has demonstrated a critical pathological role of oxysterol receptor GPR183 in various inflammatory and autoimmune diseases, including inflammatory bowel disease (IBD). However, the currently reported GPR183 antagonists are very limited and not qualified for in vivo studies due to their inferior druglike properties. Herein, we conducted a structural elaboration focusing on improving its PK and safety profile based on a reference antagonist NIBR189. Of note, compound 33, bearing an aminobenzothiazole motif, exhibited reduced hERG inhibition, improved PK properties, and robust antagonistic activity (IC50 = 0.82 nM) with high selectivity against GPR183. Moreover, compound 33 displayed strong in vitro antimigration and anti-inflammatory activity in monocytes. Oral administration of compound 33 effectively improved the pathological symptoms of DSS-induced experimental colitis. All of these findings demonstrate that compound 33 is a novel and promising GPR183 antagonist suitable for further investigation to treat IBD.
研究领域:
药理学
·
免疫学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）