INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhe Dou,Pei-Ru Hu,Lin-Lin Song,Yi-Cheng Wang,Hao-Nan Tang,Zhen-Qi Weng,Ya-Jun Wang,Yuan-Qiang Cai
Carbonyl reductases (CRs) are promising biological macromolecules for the asymmetric synthesis of chiral secondary alcohols. A novel CR was evolved for efficient conversion of d -xylose into anti-aging ( S )-Pro-Xylane. Through extensive gene mining, an active carbonyl reductase Zm CR was identified from Zygofabospora marxiana with high reductive activity towards d -xylose-derived 1-C-(β- d -xylopyranosyl)-acetone (XPSA) and in situ cofactor regeneration capability. Using a three-round of concise engineering strategy, a hextuple mutant C85V/V128C/S129A/V161S/Y166H/T217A (M6) was identified from merely 157 mutants with specific activity of 46.00 U·mg −1 , about 306 folds of WT. The Shannon-Wiener index of M6 was 2.21, much higher than WT, indicating its extended substrate scope. M6 could efficiently catalyze the asymmetric reduction of 190 g·L −1 XPSA into ( S )-XPSP. The k cat / K M XPSA of M6 was 203.84 s −1 ·mM −1 , about 910 folds of WT. More non-bonded interactions were monitored in M6, contributing to the stabilization of reactive catalytic conformation. This study provides a promising biocatalyst for the chemoenzymatic synthesis of value-added anti-aging ( S )-Pro-Xylane from lignocellulosic d -xylose.
研究领域:
生物化学
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酶工程
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合成生物学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2025
·
IF 21.1
Su Li,Jinkai Qiu,Zijun Bian,Bin Shao,Zhicheng Liu,Cheng Lian,Honglai Liu,Jun Hu
The dry reforming of carbonate with methane (CaDRM) can be one of the breakthrough technologies to address the challenge of mitigating inevitable CO 2 emissions into value-added chemicals but remains great challenges for efficient catalysts design. In this study, we break the trade-off between the catalytic activity and the carbon deposit by tailoring the d-band center of Ni through Fe doping, which matches the energy level of CH 4 * dehydrogenation but is far away from that of the over cleavage of CH* . In this light, the porous spherical Fe x Ni/Al 2 O 3 catalysts with Ni 3 Fe alloy nanoaggregates are obtained through a one-step spray drying method. The optimized Fe 0.6 Ni/Al 2 O 3 catalyst shows an excellent catalytic activity with a superior CO selectivity of 91.75 % at a complete CaCO 3 conversion at 700°C, and successfully prevents the carbon deposit to maintain its excellent stability. Moreover, the extremely low activation energy of 45.2 kJ/mol and the good porosity of Fe x Ni/Al 2 O 3 ensure its fast dynamics. Therefore, this tailoring d-band center approach through the formation of alloy catalysts supplied a novel strategy for effectively cracking hydrogen species while avoiding over cleavage of CH x * in the thermal catalysis, which promoted the CaDRM technology to be a promising solution for carbon neutrality in industrial sectors in near future.
研究领域:
化学工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Current Molecular Pharmacology
·
2024
·Vol. 17
·期 1
·e18761429267000
·
IF 2.4
Fengyan Tang,Bo Zhao,Li Zhang,Faisal Raza,Hajra Zafar,Shao Zhong,Lin Li,Wenhua Zhu,Lingna Fang,Bing Lu,Liwen Shen,Ping Guo,Nengxing Yu,Quanmin Li
Objective: The potential mechanism underlying the protective effect of Astragaloside IV (AS-IV) co-treatment with 1, 25-dihydroxy-vitamin D (Vit-D) on neuropathy in diabetic high-fat rats was investigated. Methods: The rat diabetic hyperlipidemia (DH) model was established via streptozotocin and a high-fat diet (HFD). After co-treatment (of AS-IV and Vit-D at respective doses of 50 mg/kg via oral gavage and 30000 IU/kg via intramuscular injection), blood glucose levels, markers of inflammation and oxidative stress, as well as apoptosis and histopathology were evaluated with appropriate techniques. Results: Co-treatment could effectively reduce blood glucose levels substantially (p< 0.01), improve weight loss, and decrease oral glucose tolerance. Reduced respective sensory and motor nerve conduction velocities in rats were substantially improved (p<0.01) after co-treatment. Also, we observed obvious improvement in DH-induced injured nerve fiber myelin structure and other organ pathologies in co-treated rats. Besides, we observed up-regulated expressions of peroxisomal-proliferator activated receptor-alpha (PPAR-α) and Vit-D receptors (VDR) (p< 0.01) through the western blotting technique. Using the same technique, we also discovered reduced levels of interleukin (IL)1 beta, IL-6, and tumor necrosis factor-alpha, coupled with increased IL-10 and superoxide dismutase levels (p< 0.01). Importantly, co-treatment could effectively exert anti-oxidative and anti-inflammatory effects. Also, co-treatment resulted in the up-regulation of PPAR-α and VDR expressions, inhibition of the renin–angiotensin–aldosterone system, and promotion of β-cell sensitivity to insulin. Conclusion: The combined application of AS-IV and Vit-D exhibited health effects such as anti-oxidation, regulation of inflammatory factors, and promotion of cell repair, which may be considered as the mechanisms underlying treatment of diabetic peripheral neuropathy and improvement in biochemical indicators.
研究领域:
药理学
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内分泌学
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神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEUROTOXICOLOGY
·
2018
·
IF 3.076
Jing Zheng,Juan Chen,Xiaohan Zou,Fang Zhao,Mengqi Guo,Hongbo Wang,Tian Zhang,Chunlei Zhang,Wei Feng,Isaac N. Pessah,Zhengyu Cao
Saikosaponins (SSs) are a class of naturally occurring oleanane-type triterpenoid saponins found in Radix bupleuri that has been widely used in traditional Chinese medicine. As the main active principals of Radix bupleuri , SSs have been shown to suppress mouse motor activity, impair learning and memory, and decrease hippocampal neurogenesis. In the present study, we investigated the effect of five SSs (SSa, SSb1, SSb2, SSc, and SSd) on neuronal viability and the underlying mechanisms in cultured murine neocortical neurons. We demonstrate that SSa, SSb1 and SSd produce concentration-dependent apoptotic neuronal death and induce robust increase in intracellular Ca 2+ concentration ([Ca 2+ ] i ) at low micromolar concentrations with a rank order of SSd > SSa > SSb1, whereas SSb2 and SSc have no detectable effect on both neuronal survival and [Ca 2+ ] i . Mechanistically, SSd-induced elevation in [Ca 2+ ] i is the primary result of enhanced extracellular Ca 2+ influx, which likely triggers Ca 2+ -induced Ca 2+ release through ryanodine receptor activation, but not SERCA inhibition. SSd-induced Ca 2+ entry occurs through a non-selective mechanism since blockers of major neuronal Ca 2+ entry pathways, including L-type Ca 2+ channel, NMDA receptor, AMPA receptor, Na + -Ca 2+ exchanger, and TRPV1, all failed to attenuate the Ca 2+ response to SSd. Further studies demonstrate that SSd increases calcein efflux and induces an inward current in neocortical neurons. Together, these data demonstrate that SSd elevates [Ca 2+ ] i due to its ability to increase membrane permeability, likely by forming pores in the surface of membrane, which leads to massive Ca 2+ influx and apoptotic neuronal death in neocortical neurons.
研究领域:
神经科学
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药理学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Bei-Bei Jiang,Xue-Fei Sun,Lin Wang,Si-Yu Wang,Ru-Dong Liu,Shu-Guang Wang
Biofouling is a critical issue in membrane separations . Development of novel approaches for biofouling mitigation is of crucial importance for membrane-based technologies . In this research, D-tyrosine was immobilized on a polyethersulfone (PES) ultrafiltration membrane (UF) to mitigate biofouling with the synergistic effects of surface hydrophilicity and anti-microbial properties. Results showed that the grafting of D-tyrosine increased the surface hydrophilicity and imparted a smoother surface to the membrane without altering its transport properties. During bovine serum albumen (BSA) filtration tests, the unified membrane fouling index (UMFI) of D-tyrosine-polydopamine (PDA)/PES membrane was only 0.00162, probably half of the PDA/PES membrane’s UMFI without D-tyrosine. The flux recovery ratio (FRR) of the D-tyrosine-PDA/PES membrane was up to 79%, higher than the virgin PES membrane. Moreover, the D-tyrosine-PDA/PES membrane showed better anti-biofouling performance when inoculated with E. coli culture. The proposed membrane surface modification technique paves the way toward realization of biological control for mitigating biofouling by simultaneously enhancing the anti-adhesive and antimicrobial properties of the membrane.
研究领域:
环境科学
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材料科学
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生物学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
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2015
·
IF 4.074
Cong Chen,Yan Meng,Sheng Li,Wenhua Wu,Chuanjun Liu,Xiaojuan Xu,Lina Zhang
A polysaccharide coded as PR-CA was extracted from Polyporus rhinoceros and determined to be a β-(1→3)- d -glucan with multiple branches. The weight-average molecular weights ( M w ) of PR-CA in dimethylsulfoxide (DMSO) and in water were determined with static light scattering (SLS) to be 3.57 × 10 5 and 1.79 × 10 7 , indicating existence of the single chains in DMSO and co-existence of single chains and aggregates in water. Moreover, the stiffness of single chains of PR-CA in water was directly visualized by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The hollow structure of PR-CA nanofibers with width of 30–40 nm and length of ∼350 nm formed in the water/DMSO (9:1, v:v) was demonstrated by a fluorescent probe tetraphenylethylene (TPE) via aggregation-induced emission (AIE). The formation of PR-CA nanofibers was ascribed to the parallel aggregation of the extended PR-CA chains due to the hydrogen bonding and hydrophobic interaction. This work offered valuable results for promising applications of natural branched β-glucans in the biological fields of drug inclusion, delivery and disease diagnosis.
研究领域:
生物化学
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材料科学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 3
·037506
·
IF 4.386
Huipeng Hou,Shanshan Tang,Wei Wang,Miao Liu,Axin Liang,Liquan Sun,Aiqin Luo
The present work describes a faithful strategy of dual-template molecularly imprinted polymers (MIP) to chiral recognize and quantify D-Cystine (D-Cys) and L-Cystine (L-Cys) at ultra-trace level through "vector method." Briefly, one has to saturate association D-Cys while analyzes L-Cys, and vice versa. Herein, the working electrode, magnetic glassy carbon (MGCE), was initially drop-coated with molybdenum disulfide-ionic liquid (MoS2-IL) dispersion liquid for large specific surface area and excellent electrical conductivity. After the working electrode was dried naturally, chitosan (CS) was electrodeposited on. And the strong electrostatic interactions between CS and Fe[(CN)6]3−/4− could further enhance the electric signal. Next, dual-template MIP dispersion liquid was drop-coated on the working electrode. Moreover, Fe3O4 nano-particles (NPs) was regard as the support skeleton material of dual-template MIP, which could significantly improve the bonding strength with MGCE. Herein, the proposed sensor demonstrated good analytical figures of merits with Differential Pulse Voltammetry (DPV), showing that the LOD of L-Cys and D-Cys were 0.7402 pg ml−1 and 0.6136 pg ml−1 respectively, with linear response ranges from 1 pg ml−1 to 12 pg ml−1 for both enantiomers. Furthermore, the proposed sensor exhibited great potential in chiral recognition and biochemical analysis.
研究领域:
分析化学
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生物化学
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材料科学
✓ 已验证
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2020
·
IF 3.448
Jia-Qin Wu,Xiao-Mei Xu,Ding-Lin Wang,Neng-Bing Long,Rui-Feng Zhang
Novel nano-composites were prepared by coating epoxy resin-based cationic polymer in nano-thickness via in-situ curing on the nano-wall of macroporous SiO 2 with pore size of 0.5∼1 μm. By changing the thickness of polymer coating the specific surface area and porosity varied in range of 115∼74 m 2 /g and 90.4∼83.9 %, respectively. Through ion exchange phospholipase D (PLD, from Streptomyces sp ) was efficiently immobilized on the nano-composites as support and the immobilized PLD was applied for the highly efficient synthesis of phosphatidylserine (PS). The loading amount of PLD on the nano-composited support reached to a maximum of 90.2 mg/g support , 4 times as high as that on the pure macroporous silica. The specific activity of the immobilized PLD reached as high as 16,230 U/g protein , while that of free PLD was 18,780 U/g protein . Under a wide range of temperature and pH the stability and activity of the immobilized PLD were greatly improved as compared with the free ones. Under optimized conditions at 45 °C and pH 7.0, the PS yield reached as high as 96.2 % within 40 min. After 28 days storage the immobilized PLD retained 82.2 % of original activity, and after 12 cycling reuses it retained 79.3 % of PS yield, which indicated that the immobilized PLD exhibited good stability.
研究领域:
生物化学
·
酶工程
✓ 已验证
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2024
·
IF 4.4
Lixin Liu,Yuning Wang,Jiuyi Sun,Yunan Zhang,Xiangyu Zhang,Lili Wu,Yingli Liu,Xuan Zhang,Yidi Xia,Qiumei Zhang,Ning Gao
To improve the solubility of the fluoroquinolone drug fleroxacin (FL), based on the previous experience of our research group in synthesizing co-crystals/salts of quinolone drugs to improve the physicochemical properties of drugs, Fleroxacin-D-tartaric acid dihydrate salt (FL-D-TT, C 17 H 19 F 3 N 3 O 3 ·C 4 H 5 O 6 ·2(H 2 O)), was synthesized for the first time using fleroxacin and D/L-tartaric acid (D/L-TT). Structural characterization of FL-D-TT was carried out using single-crystal X-ray diffraction, infrared spectral analysis (FT-IR) and powder X-ray diffraction (PXRD). Molecular electrostatic potential analysis showed that D-tartaric acid interacted more readily with FL than L-tartaric acid. The solubility of FL-D-TT (9.71 mg/mL, 1.82 mg/mL) was significantly higher compared to FL (0.39 mg/mL, 0.71 mg/mL) in water and buffer solution at pH 7.4. This may be attributed to the formation of charge-assisted hydrogen bonds (CAHBs) between FL and D-TT that facilitates the dissociation of FL cations in the dissolution medium, leading to an increase in FL solubility. This also led to some improvement in the in vitro antimicrobial activity of FL-D-TT against E. coli , S. typhi , and S. aureus. In addition, the hygroscopic stability of FL has been improved. Surprisingly, FL-D-TT had better photostability than FL, which could be attributed to the introduction of D-TT to make the photosensitizing moiety of FL more stable, which led to the improvement of the photostability of FL.
研究领域:
药物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CATALYSIS
·
2025
·
IF 6.5
Huabin Lian,Riming Hu,Lin Zheng,Daowei Gao,Shuai Wang,Rongyao Wang,Bin Wang,Guozhu Chen
The electrocatalytic reduction of biomass-derived furfural represents a sustainable pathway for the production of furfuryl alcohol and 2-methylfuran, serving respectively as value-added chemicals and biofuels. However, the production of these high-value-added products under mild conditions is hampered by low Faradaic efficiency. In this study, we develop a strategy to establish strong metal-support interactions, enabling Cu/NC (nitrogen-doped carbon) catalysts to efficiently produce value-added products under mild conditions. Specifically, we prepare a cost-effective NC support with highly dispersed ultrafine nano-Cu particles of approximately 1 nm in diameter. Under mild pH conditions, our comparative experiments show that the Faradaic efficiency for value-added products (furfuryl alcohol and 2-methylfuran) is nearly four times higher than that of commercial foam copper. We elucidate the influence of nitrogen-doped supports on the behavior of the supported metal Cu. Comprehensive in-situ infrared reflection absorption spectroscopy characterization and DFT theoretical calculations reveal a positive shift in the d-band center of the Cu 3d orbitals. This shift enhances the adsorption of furfural C1=O1 bonds on the Cu/NC catalyst and improves its capacity to bind active hydrogen, thereby increasing the Faradaic efficiency of the value-added products. Our findings advance theoretical understanding and providing practical insights for electrocatalytic hydrogenation of furfural under mild pH conditions.
研究领域:
化学工程
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材料科学
·
催化反应
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Qiming Chen,Jinghan Zhang,Qicheng Zhang,Bin Chen,Wenchao Peng,Yang Li,Xiaobin Fan
The rational design of tailored surface properties by surface doping is an efficient yet challenging method for enhanced OER performance. Herein, we propose atom migration effect, induced by surface O-doping to precisely control surface compositions of FeNiS 2 (FNS). The FNS undergoes a surface sulfur/oxygen exchange to form FeNiS 2-x O x (FNSO) structure after surface O-doping, while the bulk phases remain unchanged. In contrast, the surface Ni/Fe ratio is significantly increased due to the accelerated atom migration of nickel during surface O-doping. At elevated temperatures, the bulk phase is prone to transition into complex phases of FeNiS and FeNiO, leading to the decreased surface Ni/Fe ratio. The optimized FNSO demonstrates superior OER performance with a low overpotential of 256 mV at 100 mA cm −2 , and the assembled anion exchange membrane water electrolyzer requires only 1.83 V at 1 A cm −2 with robust durability. Theoretical calculations reveal that this surface atom migration adjusts the d-band center level, optimizes surface adsorption/desorption behaviors, and expedites the OER kinetics. This work contributes to a deeper comprehension of surface modulation with desired surface properties of sulfides and other compounds for enhanced OER performance.
研究领域:
材料科学
·
电化学
·
理论计算
✓ 已验证
DOI
高影响因子
Journal of Magnetic Resonance Open
·
2025
·
IF 2
Bowen Han,Jing He,Shaohua Huang
Chiral amino acids play an indispensable role in living organisms. Diffusion-ordered NMR spectroscopy is an effective NMR tool and a noninvasive analytical method for the analyses of mixture without the need for physical separation of the analytes. However, conventional diffusion-ordered NMR spectroscopy method usually fails to resolve the mixtures of chiral amino acids because of their same molecular masses, sizes, and shapes. Microcrystalline cellulose has been gradually gained more and more interest owing to its wide compatibility, surface area, excellent separation efficiency, non-toxicity, cost-effective, and mechanical stability in many fields. Herein we provide a fast, simple, specific and sensitive method to resolve some D and L-amino acids mixtures by using microcrystalline cellulose as a matrix. This work recognized some D and L- proteinogenic amino acid enantiomers with diffusion-ordered NMR for the first time.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）