INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 6
·3458-3468
·
IF 3.3
Mingyan Liu,Xingzi Yao,Gaowei Zhang,Shengxiang Yi,Xueyong Huang,Fangjian Ning,Liping Luo
Summary Eurya honey (EH) is produced from the nectar of Eurya. In this paper, the physicochemical properties (moisture, free acidity, pH, 5-hydroxymethylfurfural, conductivity, sugar, and colour) of EH were systematically studied for the first time. The total phenols and flavonoids were determined by spectrophotometry, and the contents were 40.29 ± 4.17 mg GAE/100 g and 12.44 ± 0.62 mg RE/100 g, respectively. A total of 232 compounds were identified, and 30 of them were quantified by ultra-performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-Q/TOF-MS). The potential markers of EH were abscisic acid (21.3–103.4 μg/100 g), gallic acid (6.9–220.2 μg/100 g), tricoumaroyl spermidine (284.2–518.1 μg/100 g), and kaempferol (15.0–258.7 μg/100 g). EH showed antioxidant capacities in DPPH free radical-scavenging activity (IC 50 : 42.87–131.26 mg mL −1 ), ferric reducing antioxidant capacity (23.22–112.24 μmol FeSO 4 ·7H 2 O/100 g), and ABTS radical-scavenging activity assays (9.51–31.21 μmol Trolox/100 g).
研究领域:
食品科学
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分析化学
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药理学
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农学
✓ 已验证
产品关联
DOI
高影响因子
Biomass Conversion and Biorefinery
·
2023
·
IF 4
Wen Yao,Zeng Xiang,Tan Hongyu,Liu Bing
To research the optimization of ultrasonic-assisted extraction of total flavonoids from Cortex Lycii and its biological activities. Box-Behnken design experiment was used to optimize the extraction process, and AB-8 macroporous resin was used for purification. Its biological activities were studied by the inhibition rate of α -glucosidase and α - amylase and the scavenging rate of DPPH and ABTS. The optimal extraction parameters of total flavonoids from Cortex Lycii were as follows: the extraction temperature was 63 ℃, the ethanol volume fraction was 71%, the solid–liquid ratio was 28 mL/g, the extraction time was 61 min. Under this optimal condition, the yield of total flavonoids was 1.674 mg/g, which was consistent with the predicted value of 1.637 mg/g, and was 5.85 times higher than that of the traditional water extraction method. The inhibitory rate (IC 50 ) of total flavonoids extract from Cortex Lycii on α -glucosidase was 3 μg/mL and on α -amylase was 5 μg/mL. The scavenging rate (IC 50 ) of DPPH radical was 0.648 mg/mL and the scavenging rate (IC 50 ) of ABTS radical IC 50 was 0.012 mg/mL. The optimized extraction process with response surface design was reliable, which could be used for the extraction of total flavonoids from Cortex Lycii . It has strong hypoglycemic and antioxidant activities and has a good prospect for future utilization and development.
研究领域:
药用植物学
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药学
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生物化学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Nan Sun,Jiayan Xie,Jianhua Xie,Yi Chen,Xiaobo Hu,Qiang Yu
In this study, the static adsorption–desorption performance of seven macroporous resins for bound polyphenols from mung bean coat dietary fiber (MBDF-BP) was compared, and NKA-9 macroporous resin was preferably screened for subsequent separation and purification. The composition of the purified products was identified and quantified, 44 major compounds were detected, with the main phenolic acid being p -hydroxybenzoic acid, which contained up to 8881.90 μg/g DW. The purification enriched flavonoids, with high contents of catechin (1419.03 μg/g DW) and vitexin (615.88 μg/g DW). The MBDF-BP purified products (pMBDF-BP) produced significant reversible inhibitory activity against α -glucosidase in a mixed-type inhibition manner, which was superior to the reported crude extracts. The antioxidant activity assays showed that pMBDF-BP exhibited distinct scavenging effects on DPPH•, ABTS + •, •OH free radicals, as well as reactive oxygen species (ROS) in Caenorhabditis elegans ( C. elegans ). These results demonstrated that NKA-9 macroporous resin could effectively enrich MBDF-BP extracts and enhance its antioxidant activity, which was promising to explore new sights into the applications of bound polyphenols from mung bean coat dietary fiber in functional foods or dietary supplements, thus contributing to the scientific utilization of mung bean coat resources and increasing the added value of related products.
研究领域:
食品科学
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植物化学
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生物化学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Yue Tang,Lei Xu,Zhiyang Yu,Sufen Zhang,Enguang Nie,Haiyan Wang,Zhen Yang
This study investigated the effect of electron beam irradiation (EBI) on the lipid stability of oat and barley during long-term storage. Results showed that the initial free fatty acid content in oat was higher than that in barley. This may mean that lipid hydrolysis started under the function of lipase when oat and barley were milled into flours. Both storage and EBI factors influenced lipid-degrading enzyme activity and promoted lipid oxidation in oat and barley. However, it seemed that storage had higher impacts because the DPPH scavenging activity decreased greatly, and the contents of both malondialdehyde and volatile lipid oxidation products increased in all samples. Thus, the antioxidant capacity and level of lipid oxidation after EBI treatment should be considered when producing oat and barley foods. Overall, this study shows the high potential of EBI for use as a non-thermal technique in stabilising the storage quality of oat and barley.
研究领域:
食品科学
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辐射技术
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiao Yu,Yanan Chen,Zhiguo Qi,Quan Chen,Yujin Cao,Qingshan Kong
The antioxidant activity of corn peptides is related to their molecular weight and structure. Corn gluten meal (CGM) was hydrolyzed using a combination of Alcalase, Flavorzyme and Protamex, and the hydrolysates were subjected to antioxidant activity analysis after further fractionation. Corn peptides with molecular weights less than 1 kDa (CPP1) exhibited excellent antioxidant activity. A novel peptide, Arg-Tyr-Leu-Leu (RYLL), was identified from CPP1. RYLL displayed preferable scavenging capacities for ABTS radicals and DPPH radicals, with IC 50 values of 0.122 mg/ml and 0.180 mg/ml, respectively. Based on quantum calculations, RYLL had multiple antioxidant active sites, and tyrosine was the main active site due to the highest energy of the highest occupied molecular orbit (HOMO). Moreover, the simple peptide structure and hydrogen bond network of RYLL contributed to the exposure of the active site. This study elucidated the antioxidant mechanism of corn peptides, which could provide an understanding for CGM hydrolysates as natural antioxidants.
研究领域:
食品科学
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生物化学
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药学化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Qingxia Yuan,Jing Zhang,Chulei Xiao,Chaligan Harqin,Mingyang Ma,Tao Long,Zhenghui Li,Yanling Yang,Jikai Liu,Longyan Zhao
A novel heteropolysaccharide with about twenty sugar residues named DF80-2 was obtained from Angelica pubescens Maxim. f. biserrata Shan et Yuan root, one of the most widely used traditional Chinese medicines for thousands of years in China. The possible structure of DF80-2 was proposed considering the comprehensive results of physicochemical properties, methylation analysis, and 1D/2D NMR spectroscopy, which showed that its main chain was composed of (1→3)-, and (1→4)-linked-α- d -Glc p , (1→4)-linked-β- d -Gal p , (1→6)-linked-α- d -Man p , and (1→3)-linked-α- l -Ara f , and the branch was present as the α- d -Glc p -(1→3)-β- d -Gal p A disaccharides stretched from O -6 position of (1→4)-linked-α- d -Glc moiety in the main chain. Congo red analysis, scanning electron microscopy and atomic force microscopy showed that DF80-2 possessed a triple helical conformation, and its branched monomers were interlaced with one another forming a regular network structure. DF80-2 exhibited antioxidant activity by effectively scavenging DPPH, and hydroxyl radicals, and chelating ferrous ions.
研究领域:
生药学
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天然产物化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2019
·Vol. 7
·期 3
·949-958
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IF 1.747
Yaqiong Zhang,Ming Li,Hang Gao,Bo Wang,Xu Tongcheng,Boyan Gao,Liangli (Lucy) Yu
In this study, a new red sorghum variety (Ji Liang No. 1) was investigated for its triacylglycerol (TAG) and fatty acid profiles, carotenoid and tocopherol compositions, total phenolic, total flavonoid and phenolic acid contents, and antioxidant and anti-inflammatory properties. A total of 17 TAGs were identified in the red sorghum oil. Linoleic and oleic acids were the primary fatty acids, contributing more than 80% of the total fatty acids. β-Carotene was the primary carotenoid at a level of 26.14 μg/g. α-, γ-, and δ-tocopherols were at levels of 0.19, 4.08, and 0.10 μg/g, respectively. Moreover, acetone–water (60:40, v/v) extract of the red sorghum exhibited the greatest total phenolic content of 2.77 mg GAE/g and total flavonoid content of 5.44 mg RE/g. The extract had scavenging capacities against DPPH, ABTS + , and peroxyl radicals and suppressed LPS stimulated IL-1β, IL-6, and COX-2 mRNA expressions in a dose-dependent manner. Ferulic, p -coumaric, isoferulic, and p -hydroxybenzoic acids were found in the red sorghum, with ferulic acid as the predominant phenolic acid and mostly in an insoluble bound form. These data indicated a potential utilization of the red sorghum in health-promoting functional food or supplemental products.
研究领域:
农业
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食品科学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2024
·
IF 6.6
Tao Jiang,Weijian Chen,Chao Lu,Jiyong Yang,Ziquan Zeng,Wenqiang Li,Hongsheng Liu,Nana Huang,Yuhui Chen,Wengang Liu
Background Skin wounds are a prevalent issue that can have severe health consequences if not treated correctly. Nanozymes offer a promising therapeutic approach for the treatment of skin wounds, owing to their advantages in regulating redox homeostasis to reduce oxidative damage and kill bacteria. These properties make them an effective treatment option for skin wounds. However, most of current nanozymes lack the capability to simultaneously address inflammation, oxidative stress, and bacterial infection during the wound healing process. There is still great potential for nanozymes to increase their therapeutic functional diversity and efficacy.Methods Herein, copper-doped hollow mesopores cerium oxide (Cu-HMCe) nanozymes with multifunctional of antioxidant, antimicrobial and pro-vascularity is successfully prepared. Cu-HMCe can be efficiently prepared through a simple and rapid solution method and displays sound physiological stability. The biocompatibility, pro-angiogenic, antimicrobial, and antioxidant properties of Cu-HMCe were assessed. Moreover, a full-thickness skin defect infection model was utilized to investigate the wound healing capacity, as well as anti-inflammatory and pro-angiogenic properties of nanozymes in vivo.Results Both in vitro and in vivo experiments have substantiated Cu-HMCe’s remarkable biocompatibility. Moreover, Cu-HMCe possesses potent antioxidant enzyme-like catalytic activity, effectively clearing DPPH radicals (with a scavenging rate of 80%), hydroxyl radicals, and reactive oxygen species. Additionally, Cu-HMCe exhibits excellent antimicrobial and pro-angiogenic properties, with over 70% inhibition of both E. coli and S. aureus. These properties collectively promote wound healing, and the wound treated with Cu-HMCe achieved a closure rate of over 90% on the 14th day.Conclusion The results indicate that multifunctional Cu-HMCe with antioxidant, antimicrobial, and pro-angiogenic properties was successfully prepared and exhibited remarkable efficacy in promoting wound healing. This nanozymes providing a promising strategy for skin repair.
研究领域:
纳米技术
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生物医学工程
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伤口愈合
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Ziyan Chen,Yan Zhao,Mengke Zhang,Xiufang Yang,Pengxiang Yue,Dikang Tang,Xinlin Wei
A new polysaccharide (pFSP) was first isolated from the originally discarded fibrous roots part of Chinese traditional herb, Bletilla striata. pFSP was composed of D-glucose, D-galactose and D-mannose in a molar ratio of 1: 2.03: 3.45 with molecular weight of 9.1 × 10 4 Da. It could effectively scavenge DPPH and superoxide radicals with inhibition rate of 64.47% and 72.27% at 5.0 mg/ml, higher than that of polysaccharide from Bletilla striata tuber. Structural investigations of the periodate oxidation studies and Smith-degradation as well as the FT-IR spectroscopy were performed, and combined with 1D and 2D NMR spectroscopy, the repeating unit of pFSP contained (1→4)-linked-α-D-Glcp, (1→4)-linked-β-D-Manp and (1→3,6)-linked-β-D-Manp units, together with the branches of (1→6)-linked-β-D-Galp and terminated with (1→)-linked-β-D-Manp residue.
研究领域:
药理学
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生物化学
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分子生物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Wuxia Zhang,Dan Song,Dan Xu,Tingting Wang,Lu Chen,Jinyou Duan
Rhizoma Acori Tatarinowii is widely used in traditional Chinese medicine. In this study, three novel polysaccharides designated RATPW, RATPS1 and RATPS2 were isolated from Rhizoma Acori Tatarinowii by DEAE-52 cellulose chromatography. Their structures were characterized using physicochemical and spectral methods. Chemical analysis indicated that RATPW (6.5 × 10 3 Da) mainly composed of glucose and fructose. RATPS1 (1.5 × 10 5 Da) contained galactose and arabinose, while RATPS2 (5.3 × 10 4 Da) contained ∼49.5% galacturonic acid along with rhamnose, fructose, galactose, and arabinose. In vitro , RATPS2 showed the most significant scavenging activity on DPPH and hydroxyl radical. Three polysaccharides could protect the PC12 cells from H 2 O 2 -induced damage. Immunological tests indicated that both RATPW and RATPS2 significantly stimulated NO production and phagocytic activity in RAW264.7, and promoted splenocyte proliferation. These data suggested that polysaccharides RATPW and RATPS2 had the potential as novel natural sources of antioxidative and immunopotentiating agents.
研究领域:
药理学
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生物化学
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免疫学
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传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2016
·
IF 3.182
Cen Zhang,Guowen Zhang,Junhui Pan,Deming Gong
Galangin is a natural flavonol isolated from plants with potent biological activities. Galangin was found to significantly inhibit xanthine oxidase (XOD) activity in a competitive manner with the generation of superoxide radical (O 2 − ) in the enzyme catalysis, but galangin showed insignificant scavenging activity on 1,1-diphenyl-2-picryhydrazyl (DPPH) and O 2 – radicals. These results demonstrated that inhibition of O 2 – radical generation by galangin may be due to the competitive inhibition of uric acid formation by a ping-pong mechanism. XOD had one high affinity binding site for galangin with a binding constant of 3.60 × 10 4 L mol − 1 at 298 K. Hydrogen bond and hydrophobic interaction dominated the binding process on account of the negative enthalpy and positive entropy changes. The binding of galangin to XOD induced an increase in α-helix and random coil contents and a decrease in β-sheet and β-turn contents of XOD. Further molecular docking study validated that galangin can competitively bind to the site in the molybdenum atomic (Mo) domain, occupying the catalytic center to avoid the entrance of the substrate xanthine, resulting in the inhibition of XOD activity. These findings have provided new insights into the two-substrate kinetics of galangin on XOD and useful information for functional research of galangin in the treatment of gout and oxidative damage.
研究领域:
药理学
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生物化学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PLoS One
·
2013
·Vol. 8
·期 7
·e71144
·
IF 3.73
Yihai Wang,Limin Xiang,Chunhua Wang,Chao Tang,Xiangjiu He
The antidiabetic and antioxidant activities of the ethyl acetate-soluble extract (MFE) of mulberry fruit (Morus alba L.) were investigated. In vitro , MFE showed potent α-glucosidase inhibitory activity and radical-scavenging activities against DPPH and superoxide anion radicals. In vivo , MFE could significantly decrease fasting blood glucose (FBG) and glycosylated serum protein (GSP), and increase antioxidant enzymatic activities (SOD, CAT, GSH-Px) in streptozotocin (STZ)-induced diabetic mice. Bioactivity-guided fractionation of the MFE led to the isolation of 25 phenolic compounds, and their structures were identified on the basis of MS and NMR data. All the 25 compounds were isolated from mulberry fruit for the first time. Also, the α-glucosidase inhibitory activity and antioxidant activity of the phenolics were evaluated. Potent α-glucosidase inhibitory and radical-scavenging activities of these phenolics suggested that they may be partially responsible for the antidiabetic and antioxidant activities of mulberry fruit.
研究领域:
药理学
·
生物化学
·
营养学
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）