ADVANCED SYNTHESIS & CATALYSIS
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2026
·Vol. 368
·期 15
·e70681
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IF 3.7
Yating Zou,Jinghui Zhou,Yongyi Zeng,Zhimiao Luo,Bishuang Chen,Lan Liu,Gang Xu
Ene-reductases (ERs) catalyze stereoselective anti-addition to activated alkenes, a mechanism that intrinsically favors trans products and appears incompatible with alkyne semihydrogenation, particularly for the formation of ( Z )-alkenes. Here, we investigate how ERs achieve both ( E )- and ( Z )-selective alkyne reduction within this conserved catalytic framework. Screening of a 51-member ER library reveals distinct kinetic partitioning between alkyne-to-alkene and alkene-to-alkane reduction steps, governed by differential stabilization of the substrate and intermediate within the active site. Guided by this insight, engineering of OYE3 suppresses over-reduction and enables rapid, selective formation of ( E )-alkenes under optimized reaction conditions. Unexpectedly, selected ERs also produce ( Z )-alkenes from cyano-substituted alkynes. Combined mutagenesis and molecular dynamics analyses exclude noncanonical proton donors and further suggest a substrate reorientation mechanism involving a ~180° flip within the active pocket. Together, these findings point to a unified mechanistic framework that may account for how ERs generate both ( E )- and ( Z )-alkenes from alkynes, and provide a working hypothesis for the origin of stereochemical divergence in ER-catalyzed alkyne reduction.
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Device
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2025
·
IF 8
Xinyi Ke,Yifan Duan,Yifei Duan,Zhe Zhao,Chunyu You,Tingting Sun,Xingyu Gao,Ziyu Zhang,Wen Xue,Xuanyong Liu,Yongfeng Mei,Gaoshan Huang,Junhao Chu
Electronic skin (e-skin) mimics the sensing abilities of human skin and offers sensitivity and flexibility, which has garnered attention for medical health monitoring. Nonetheless, limitations in the properties of active materials and signal-to-noise ratio hinder the implementation of multi-functional detection such as integrating biomolecular sensing, motion detection, and electrocardiography in a single device. Here, we introduce a strategy of preparing a multi-functional e-skin that utilizes a composite material combining self-locking chitosan and conductive metal-organic framework film, achieving high-performance motion detection and biomolecular sensing. The integration of deep learning, specifically transformer neural networks, aids in recognizing subtle facial micro-expressions. A sensing array is crafted by utilizing a rapid assembly method that supports discerning more attributes with heightened precision.
研究领域:
生物医学工程
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材料科学
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人工智能
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Water Research X
·
2025
·
IF 8.2
Weihong Xu,Haibei Li,Zhongwei Yang,Tianjiao Chen,Shuqing Zhou,Yue Zhao,Danyang Shi,Dong Yang,Junwen Li,Min Jin
High turbidity, attributed to suspended solids and colloidal particles, can lead to microbiological control failure during water disinfection. Lignin, a naturally occurring particle suspended in water, may contribute to turbidity. However, its ability to adsorb bacteria and protect them from chlorination remains poorly understood. In this study, we investigated Escherichia coli inactivation by NaClO in the presence and absence of natural particles. Among the five tested microparticles, lignin significantly enhanced E. coli survival under NaClO exposure, diminishing its bactericidal effect. Furthermore, surviving bacteria showed upregulation of gene expression related to energy metabolism and oxidative stress, as indicated by transcriptional analysis and qPCR measurements. These findings were further supported by increased ATP content, decreased ROS levels, and a prolonged lag phase in growth curves, enabling bacteria to rapidly restore their growth activity. Further analysis revealed that E. coli binds to the surface of lignin through hydrophobic interactions involving hydroxyl, methyl, aromatic rings, and ether groups. Lignin exhibited a strong bacterial adsorption capacity, as described by pseudo-second-order (PSO) kinetics and the Freundlich model, leading to reduced membrane permeability to NaClO. Importantly, the protective effect of lignin on bacteria was positively correlated with its hydrophobicity, with greater hydrophobicity resulting in enhanced bacterial protection. These findings suggest that lignin significantly increases E. coli resistance to chlorination, potentially contributing to disinfection failure in water treatment. This study highlights the role of lignin as a potential hazard that compromises microbiological control, offering new insights into chlorination failure caused by turbidity.
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ADVANCED ENGINEERING MATERIALS
·
2025
·
IF 3.3
Yue Zhu,Liya Dai,Mingshuang Hu,Fei Wang,Haoran Guo,XiaoYun Zhao,Hui Yang
Electronic tattoos (e-tattoos), which can be conformally attached to the human skin for real-time, continuous, and noninvasive sensing of physiological signals, hold great promise for personalized healthcare applications. However, e-tattoos based on these water-soluble materials are easily degraded by sweat in the course of daily exercise, leading to the inaccuracy of human signal sensing for long-term monitoring. Herein, enhanced waterproof breathable e-tattoos via spray deposition of a water-soluble composite ink containing poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate)(PEDOT:PSS), single-walled carbon nanotubes, waterborne polyurethane, and maltitol (as a plasticizer) is developed. The sprayed e-tattoos exhibit a distinctive 3D network structure. This unique architecture endows them with exceptional waterproof properties, excellent conformal adhesion, breathability, and biocompatibility, thereby ensuring their long-term wearability. As epidermal electronic devices, the highly conformal e-tattoos serve as ion-selective interdigital electrodes and seamlessly integrated with a flexible printed circuit board. Such integration enabled wireless, continuous, and real-time monitoring of potassium ion (K + ) concentrations in human sweat during daily activities. Therefore, the waterproof and breathable e-tattoos with excellent comprehensive performance could provide a promising platform for next-generation wearable epidermal electronics.
研究领域:
材料科学
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纳米技术
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生物医学工程
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柔性电子学
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可穿戴传感器
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Journal of Ocean University of China
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2025
·Vol. 24
·期 6
·1827-1833
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IF 1.2
Xin Wenyuan,Zhou Yunbing,Liu Daixi,Wang Cong,Sherwin K. B. Sy
Astaxanthin (AX) and vitamin E (VE) are widely consumed nutritional supplements in China, with its beneficial effects predominantly attributed to all-trans AX and VE. The aim of this study is to develop and validate a rapid and accurate method for quantifying the content of AX and VE in nutritional supplement products using highly sensitive 1 H NMR method. Coumarin was chosen as the internal standard. Specific signals from AX was optimal at H-7,7′ in the chemical shift range of δ 6.17–6.24 ppm, whereas the signals of VE at δ 2.59 ppm. To demonstrate the reliability of this analytical approach the proposed method underwent rigorous validation, specificity, limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision, and recovery. The accuracy of the validation method was 3.10% for AX and 1.99% for VE. The results indicated that the method was precise and reliable. The method has been successfully applied to simultaneous quantification of AX and VE in nutritional supplements products.
研究领域:
分析化学
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营养科学
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药物分析
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IEEE SENSORS JOURNAL
·
2024
·Vol. 24
·期 11
·18327-18335
·
IF 4.3
Zihan Wang,Kai-Chong Lei,Huaze Tang,Yang Luo,Hongfa Zhao,Peisheng He,Wenbo Ding,Liwei Lin
Vibration perception can help robots recognize their dynamic states to explore the surrounding environment. However, the intrinsic stretchability of soft robots poses challenges to integrating vibration sensors. This study introduces an innovative stretchable electronic skin (e-skin) that facilitates vibration proprioception in soft robots. Constructed with a thickness of approximately 0.1 mm, this ultrathin e-skin is produced using a screen-printing technique with liquid metal particles (LMPs), incorporating a kirigami design for seamless integration. The e-skin works by the triboelectric nanogenerator-based sensing mechanism, which transduces mechanical vibration into an electrical signal without an external power source. By analyzing the vibration signals generated by the dynamic motions of soft robots, the e-skin shows a wide range of applications. From the vibration signal of the soft robotic finger’s sliding motion, 17 different textures can be distinguished with 99% accuracy. Furthermore, analysis of the vibration signal from a soft robotic gripper’s swinging motion enables the estimation of both the type and weight of grains inside the container it grips, achieving accuracies of 97.7% and 95.3%, respectively. As such, this work presents a new approach to realizing the vibration proprioception of soft robots, thereby broadening the applications of dynamic proprioception in soft robotics.
研究领域:
生物医学工程
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柔性电子
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软体机器人学
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传感器技术
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人机交互
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智能材料
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纳米能源
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Particuology
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2021
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IF 3.067
Youhong Zhang,Yinpei Zhu,Leslie S. Loo,Jianguo Yin,Kean Wang
Molecularly imprinted polymer (MIP) beads imprinted with vitamin E (V E ) were synthesized via a two-step swelling polymerization method, using methacrylic acid (MAA) and other functional monomers together with polystyrene (PS) microspheres as the substrate. In initial trials, different functional monomers were assessed, along with a variety of solvents and imprinting ratios. The MIP MAA sample exhibited the highest performance and was characterized and also assessed with regard to V E adsorption under various experimental conditions. This material exhibited reasonably good sphericity, porosity and stability, along with an excellent adsorption capacity of 46 mg/g. This was much higher than the value for the corresponding non-imprinted polymer made with the same monomer, and 20% higher than that for an acrylamide-based MIP synthesized using a precipitation polymerization method. The core-shell structured MIP MAA also demonstrated fast adsorption kinetics, a low pressure drop and good reusability in fixed bed experiments. The V E adsorption equilibrium, selectivity, kinetics, thermodynamics and regeneration capacity of this material were also examined and are reported herein.
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CHEMICAL RESEARCH IN TOXICOLOGY
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2020
·
IF 3.184
Yuhe Kan,Lu Chen,Dao Lin,Xinya Bu,Mengwu Mo,Liang Yan,Zhenjun Yang,Longfei Yuan,Li Wu,Yujian He
DNA damage can occur naturally or through environmental factors, leading to mutations in DNA replication and genomic instability in cells. Normally, natural d-nucleotides were selected by DNA polymerases. The template l-thymidine (l-T) has been shown to be bypassed by several types of DNA polymerases. However, DNA replication fidelity of nucleotide incorporation opposite l-thymidine in vivo remains unknown. Here, we constructed plasmids containing a restriction enzyme (PstI) recognition site in which the l-T lesion was site-specifically located within the PstI recognition sequence (CTGCAG). Further, we assessed the efficiencies of nucleotide incorporation opposite the l-T site and l-T lesion bypass replication in vitro and in vivo. We found that recombinants containing the l-T lesion site inhibited DNA replication. In addition, A was incorporated opposite the l-T lesion by routine PCR assay, whereas preference for nucleotide incorporation opposite the l-T site was A (13%), T (22%), C (46%), and G (19%), and no nucleotide insertion and deletions were detected in E. coli cells. In particular, a novel restriction enzyme-mediated method for detection of the mutagenic properties of DNA lesion was established, which allows us to readily detect restriction–digestion of the l-T-bearing plasmids. The study provided significant insight into how mirror-image nucleosides perturb the fidelity of DNA replication in vivo and whether they elicit mutagenic effects, which may help to understand both how DNA damage interferes with the flow of genetic information during DNA replication and development of diseases caused by gene mutation.
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高影响因子
JOURNAL OF CONTROLLED RELEASE
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2021
·
IF 9.776
Lina Liang,Yan Peng,Liyan Qiu
Inducing mitochondrial malfunction is an appealing strategy to overcome tumor multidrug resistance (MDR). Reported here a versatile mitochondrial-damaging molecule, vitamin E succinate (VES), is creatively utilized to assist MDR reversal of doxorubicin hydrochloride (DOX·HCl) via a nanovesicle platform self-assembled from amphiphilic polyphosphazenes containing pH-sensitive 1H-benzo-[d]imidazol-2-yl) methanamine (BIMA) groups. Driven by multiple non-covalent interactions, VES is fully introduced into the hydrophobic membrane of DOX·HCl-loaded nanovesicles with loading content of 23.5%. The incorporated VES also offers robust anti-leakage property toward DOX·HCl under normal physiological conditions. More importantly, upon release within acidic tumor cells, VES can target mitochondria and result in various dysfunctions including excessive generation of reactive oxygen species (ROS), mitochondrial membrane potential (Δ Ψ m ) loss, and inhibited adenosine triphosphate (ATP) synthesis, which contribute to cell apoptosis and insufficient energy supply for drug efflux pumps . Consequently, the killing-effect of DOX·HCl is significantly enhanced toward drug resistant cancer cells at the optimal mass ratio of DOX·HCl to VES. Further in vivo antitumor investigation on nude mice bearing xenograft drug-resistant human chronic myelogenous leukemia K562/ADR tumors verifies the extremely enhanced anti-tumor efficacy of the dual drug-loaded nanovesicle with the tumor inhibition rate (TIR) of 82.38%. Collectively, this study provides a s safe, facile and promising strategy for both precise drug delivery and MDR eradication to improve cancer therapy.
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Nano Energy
·
2017
·
IF 12.343
Zheng Lou,Shuai Chen,Lili Wang,Ruilong Shi,La Li,Kai Jiang,Di Chen,Guozhen Shen
The development of electronic skin is the key to the realization of artificial intelligence that immediate contacts with humans, as well as biomedical applications . To mimic the tactile sensing properties of natural skin, large ultrathin arrays of pixel high-performance pressure sensors on a flexible and stretchable substrate are required. Here, we demonstrate ultrathin, flexible multimodal sensing capability of e-skin sensor based on polyaniline hollow nanospheres composite films (PANI-HNSCF). Hollow structure endows the thin films with structure-derived elasticity and a low effective elastic modulus of 0.213 MPa. The effective amplification and transformation of various external stimuli to independent electrical signals enables the precise and continuous sensing of pressure and temperature with a high pressure sensitivity of 31.6 kPa −1 and an accurate temperature resolution of 0.08 °C −1 . Importantly, the single sensing unit can be easily integrated sensor arrays with excellent sensing performance. Our work provided the technological verify between structure of materials and properties of device and make hollow materials possess promising application in e-skin and health-monitoring elements.
研究领域:
材料科学
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生物医学工程
·
人工智能
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MICROBIOLOGICAL RESEARCH
·
2024
·
IF 6.1
Ruixiang Yang,Thiruselvam Viswanatham,Shuangqin Huang,Yuanping Li,Yanshuang Yu,Jinlin Zhang,Jian Chen,Martin Herzberg,Renwei Feng,Barry P. Rosen,Christopher Rensing
Antimony is pervasive environmental toxic substance, and numerous genes encoding mechanisms to resist, transform and extrude the toxic metalloid antimony have been discovered in various microorganisms. Here we identified a major facilitator superfamily (MFS) transporter, AntB, on the chromosome of the arsenite-oxidizing bacterium Ensifer adhaerens E-60 that confers resistance to Sb(III) and Sb(V). The antB gene is adjacent to gene encoding a LysR family transcriptional regulator termed LysR ars , which is an As(III)/Sb(III)-responsive transcriptional repressor that is predicted to control expression of antB . Similar antB and lysR ars genes are found in related arsenic-resistant bacteria, especially strains of Ensifer adhaerens , and the lysR ars gene adjacent to antB encodes a member of a divergent subgroup of putative LysR-type regulators. Closely related AntB and LysR ars orthologs contain three conserved cysteine residues, which are Cys17, Cys99, and Cys350 in AntB and Cys81, Cys289 and Cys294 in LysR ars , respectively. Expression of antB is induced by As(III), Sb(III), Sb(V) and Rox(III) (4-hydroxy-3-nitrophenyl arsenite). Heterologous expression of antB in E. coli AW3110 (Δ ars ) conferred resistance to Sb(III) and Sb(V) and reduced the intracellular concentration of Sb(III). The discovery of the Sb(III) efflux transporter AntB enriches our knowledge of the role of the efflux transporter in the antimony biogeochemical cycle .
研究领域:
微生物学
·
环境科学
·
分子生物学
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ULTRASONICS SONOCHEMISTRY
·
2016
·
IF 4.556
Lingling Zhang,Huan Qi,Zhengxu Yan,Yu Gu,Weiqiang Sun,Abraham Amenay Zewde
The present study evaluated inactivation efficiency of a sonophotocatalytic process using ZnO nanofluids including ultrasonic parameters such as power density, frequency and time. The result showed that inactivation efficiency was increased by 20% when ultrasonic irradiation was combined with photocatalytic process in the presence of natural light. Comparison of inactivation efficiency in photocatalytic, ultrasonic and sonocatalytic processes using Escherichia coli as a model bacteria identified that inactivation efficiencies are shown in the following order: ultrasonic irradiation < sonocatalysis < photocatalysis < sonophotocatalysis. Furthermore, inactivation mechanism of sonophotocatalysis was proposed. Studies of reactive oxygen species (ROS) and zinc ions (Zn 2+ ) release evaluation revealed that ROS play a key role in bacterial inactivation rather than Zn 2+ . Permeability of outer membrane (OM) and inner membrane (IM) of E. coli bacterial cells were studied and exhibited that sonophotocatalysis increased the permeability of OM and IM significantly. The enhanced bacterial inactivation effect in sonophotocatalytic process contributed to acoustic cavitation, sonocatalysis of ZnO and sonoporation phenomenon.
研究领域:
环境科学
·
微生物学
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高影响因子
危险品化学品经营许可证（带存储）