JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Bojie Yuan,Minghao Sui,Jie Qin,Jingyu Wang,Hongtao Lu
Understanding the interaction between silver nanoparticles (Ag NPs) and substances in aquatic systems enables Ag NPs to be better applied in science and industry. Moreover, it also could help us to determine the fate of Ag NPs and assess their hazards in aquatic environment. In this work, the interaction of Ag NPs with HCO 3 − , ubiquitous ligand in aquatic systems, during long-term incubation was firstly studied and the possible mechanism was proposed. Results showed that the long-term incubation with HCO 3 − led to the red-shifting of UV–vis spectra, the increasing of particles zeta potential (more negative), and the declining of the aqueous Ag + concentration, as well as the decreasing in the toxicity of Ag NPs suspensions. Furthermore, the ambient temperature was found to have a great impact on the interaction of Ag NPs with HCO 3 − . The reaction process between Ag NPs and HCO 3 − was revealed by H 2 O 2 mediated oxidation experiments. An ultrathin Ag 2 CO 3 layer was proved to form on the surface of Ag NPs, which should be the reason for the evolution of various properties of Ag NPs described above.
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FOOD CONTROL
·
2021
·
IF 5.548
Shujuan Wang,Yiling Fan,Zhen Feng,Minghui Song,Qiongqiong Li,Bo Jiang,Feng Qin,Hao Liu,Lefu Lan,Meicheng Yang
Escherichia coli O157:H7 ( E. coli O157:H7) is one of the most dangerous foodborne pathogens causing severe illnesses in the elderly, children and immunocompromised people. Traditional methods could not meet the detection requirements of high speed, sensitivity, and specificity. The clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic is a novel high sensitivity and specificity detection method. In this study, a rapid nucleic acid of Escherichia coli O157:H7 was detected using rfbE gene based on CRISPR/Cas12a system. The limit of detection for DNA and bacterial concentrations was 0.9 pg/μL and 6.5 × 10 4 CFU/mL, respectively. Furthermore, when the initial bacterial inoculum was 14 CFU/mL in the ground beef sample, the target was detected after 4 h of culture through Metal Organic Framework immunomagnetic beads (Fe 3 O 4 @ [email protected] 2 ) enrichment.
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AMB Express
·
2022
·Vol. 12
·期 1
·1-17
·
IF 4.126
Liu Shasha,She Pengfei,Li Zehao,Li Yimin,Li Linhui,Yang Yifan,Zhou Linying,Wu Yong
High incidences of urinary tract infection (UTI) of aminoglycosides-resistant E.coli causes a severe burden for public health. A new therapeutic strategy to ease this crisis is to repurpose non-antibacterial compounds to increase aminoglycosides sensibility against multidrug resistant E.coli pathogens. Based on high throughput screening technology, we profile the antimicrobial activity of tavaborole, a first antifungal benzoxaborole drug for onychomycosis treatment, and investigate the synergistic interaction between tavaborole and aminoglycosides, especially tobramycin and amikacin. Most importantly, by resistance accumulation assay, we found that, tavaborole not only slowed resistance occurrence of aminoglycosides, but also reduced invasiveness of E.coli in combination with tobramycin. Mechanistic studies preliminary explored that tavaborole and aminoglycosides lead to mistranslation, but would be still necessary to investigate more details for further research. In addition, tavaborole exhibited low systematic toxicity in vitro and in vivo, and enhanced aminoglycoside bactericidal activity in mice peritonitis model. Collectively, these results suggest the potential of tavaborole as a novel aminoglycosides adjuvant to tackle the clinically relevant drug resistant E. coli and encourages us to discover more benzoxaborole analogues for circumvention of recalcitrant infections.
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JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2020
·Vol. 20
·期 12
·7558-7568
·
IF 1.134
Chi Fenping,Chen Pengpeng,Mao Changjie
Rose Bengal (RB) was used as a functional pigment and poly dimethyl diallyl ammonium chloride was used as a coupling agent to modify Graphene Oxide (GO) in order to enhance the light absorption and ROS generation of GO. GO, RB and the obtained RB-PDDA-GO were characterized by scanning
electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, thermogravimetric analysis, Raman spectroscopy, UV-visible spectrophotometry, and X-ray photoelectron spectroscopy. The oxidation of hydroquinone to p-benzoquinone was used to evaluate the oxidation
ability. Three kinds of reactive oxygen species (O2
·-, 1O2 and ·OH) produced by the materials under light irradiation were detected by the ESR method using TEMP (2,2,6,6-tetramethyl-4-piperidine) and DMPO (5,5-dimethyl-1-pyrroline-N-oxide)
as capture agents. The results showed that RB-PDDA-GO produced more ROS under light than GO. Antibacterial experiments were carried out with E. coli as the target strain to detect the actual utility of ROS produced by the materials. The results showed that RB-PDDA-GO had a significant
sterilization effect.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2011
·Vol. 165
·期 2
·666-675
·
IF 1.879
Chen Sheng,Liu Zhiguo,Chen Jian,Wu Jing
Escherichia coli is one of the most commonly used host strains for recombinant protein production. More and more research works on the production of recombinant protein indicate that extracellular production throughout a culture medium is more convenient and attractive compared to intracellular production. In present work, inducing temperature and isopropyl β- d -1-thiogalactopyranoside (IPTG) concentration were investigated to decrease the formation of inclusion body and increase the amount of soluble recombinant cutinase initially. Enzyme activity in the culture medium reached to 118.9 U/ml at 64 h of culture, and no inclusion body was detected in cytoplasm under the inducement condition of 0.2 mM IPTG and 30°C. In addition, it was found that a large amount of cutinase had been accumulated in periplasm since 16-h cultivation under the same inducement condition. Therefore, glycine and surfactant sodium taurodeoxycholate (TDOC) were further used to promote the leakage of recombinant cutinase from periplasm. Supplied with 100 mM glycine and 1 mM TDOC, the amount of cutinase in periplasm decreased remarkably, and the activity in the culture medium reached to 146.2 and 149.2 U/ml after 54 h of culturing, respectively.
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Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Zhang Haipeng,Zhao Xiaoou,Wang Xiaoyu,Wang Xinyue,Gu Jinyang,Liu Zhongliang,Kong Lingcong,Chen Jingrui,Ma Hongxia
The widespread use of commercial antibiotics has led to the emergence of multidrug resistance in pathogenic bacteria, posing a significant threat to human health and underscoring the urgent need for alternative antimicrobial agents. In this study, a bacteriocin-producing strain, Bacillus velezensis FS-3 ( B. velezensis FS-3), was isolated from soil in Changbaishan, China. Fermentation conditions were optimized to enhance both bacteriocin activity and yield. A novel bacteriocin, PFS-3, was purified from B. velezensis FS-3 using hydrochloric acid precipitation, organic solvent extraction, and preparative reversed-phase high-performance liquid chromatography. Liquid chromatography-mass spectrometry/mass spectrometry analysis determined its molecular weight to be 929.16 Da, and amino acid sequencing of this peptide revealed eight amino acids (STYLFEGL). To date, the biological properties of PFS-3 have not been reported. We demonstrate here that PFS-3 exhibits low toxicity and remarkable stability under diverse conditions, including variations in temperature, pH, and the presence of metal ions or organic reagents. PFS-3 displayed broad-spectrum antimicrobial activity, with particularly strong activity against Gram-negative bacteria. Notably, its minimum inhibitory concentration against multidrug-resistant Escherichia coli B2 (MDR E. coli B2) was 16 μg/mL. Mechanistic investigations revealed that bacteriocin PFS-3 exerts bactericidal effects on MDR E. coli B2 by disrupting cardiolipin in the outer membrane of the cells. Furthermore, in vivo experiments demonstrated that PFS-3 significantly improved survival rates in infection models of MDR E. coli . In conclusion, PFS-3 is a newly identified bacteriocin with strong antibacterial activity, high stability, safety, and a favorable therapeutic index, highlighting its potential applications in the food industry and biopharmaceuticals for combating MDR E. coli infections.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 24
·13684
·
IF 4.9
Lankun Yi,Yubin Bai,Xu Chen,Weiwei Wang,Chao Zhang,Zixuan Shang,Zhijin Zhang,Jiajing Li,Mingze Cao,Zhen Zhu,Jiyu Zhang
Rutin is a widely known plant secondary metabolite that exhibits multiple physiological functions. The present study focused on screening for synergistic antibacterial combinations containing rutin, and further explored the mechanisms behind this synergy. In vitro antibacterial test results of rutin showed that the ranges of minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) are 0.125–1 and 0.125–2 mg/mL, respectively. However, rutin and amikacin have a significant synergistic effect, with a fractional inhibitory concentration index (FICI) range of 0.1875–0.5. The time bactericidal curve proved that the combination of rutin and amikacin inhibited bacterial growth within 8 h. Scanning electron microscopy (SEM) revealed that a low-dose combination treatment could disrupt the cell membrane ofEscherichia coli(E. coli). A comprehensive analysis using alkaline phosphatase (AKP), K+, and a protein leakage assay revealed that co-treatment destroyed the cell membrane ofE. coli, resulting in the significant leakage of AKP, intracellular K+, and proteins. Moreover, confocal laser scanning microscopy (CLSM) and red–green cell ratio analysis indicated severe damage to theE. colicell membrane following the co-treatment of rutin and amikacin. This study indicates the remarkable potential of strategically selecting antibacterial agents with maximum synergistic effect, which could significantly control antibiotic resistance.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Qiaoli Yang,Yaping Wang
This research developed an electrochemical biosensor based on T4 bacteriophages assembled with beta-galactosidase (β-gal) for Escherichia coli K12 ( E. coli K12) detection. The β-gal Soc fusion proteins were affinity-assembled on the Hoc − Soc − T4 phage capsid, exhibiting superior substrate affinity and enhanced stability compared to the free enzyme. The antibodies were immobilized on the multiwalled carbon nanotube/gold nanoparticle modified electrode, and then the engineered β-gal T4 was bonded the bacterial cells captured by the antibodies, constructing the electrochemical biosensor. The biosensor can detect E. coli K12 as low as 6 CFU/mL within 90 mins utilizing catalytic events of β-gal T4. The biosensor exhibited high specificity, distinguishing live target bacteria from non-target bacteria or dead target bacteria, which is attributed to its dual recognition mechanism involving antibody and β-gal T4. The biosensing platform can be extended to other bacteria by customizing phage-enzyme combinations, enabling bacterial detection in food, environment and other fields.
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ACS Synthetic Biology
·
2025
·
IF 3.9
Yifan Feng,Wenlong Xiao,Xinyi Li,Weiyu Cao,Yujia Jiang,Wenming Zhang,Wankui Jiang,Fengxue Xin,Min Jiang
Ectoine, a major compatible solute in halophilic micro-organisms, shows great potential in cosmetics and pharmaceuticals areas owing to its water-binding properties and capability to prevent oxidative damage. In this study, the ectABC gene cluster responsible for the ectoine synthesis originated from halophilic bacterium Halomonas venusta was first assembled into Escherichia coli. Subsequently, the crr gene in PTS was knocked out to further drive the metabolic flux from phosphoenolpyruvate to oxaloacetate, resulting in 1.27 g/L of ectoine. Then, the rate-limiting enzyme LysC in the ectoine synthesis pathway was identified and modified. The recombinant E. coli with the further overexpression of feedback-insensitive mutant EclysC* increased the ectoine titer to 2.51 g/L with a yield of 0.37 g/g in shake flasks. After the medium optimization including the carbon and nitrogen source, sodium chloride, and magnesium sulfate concentration, the ectoine titer was improved to 4.55 g/L. 115.15 g/L of ectoine with a yield of 0.23 g/g was obtained in the 5.0 L bioreactor through the optimization of substrate feeding and IPTG supplementation in the fed-batch fermentation. To achieve the cost-effective production of ectoine, lignocellulosic hydrolysate from wheat straw was adopted. 134.08 g/L of ectoine with a yield of 0.33 g/g sugar and a productivity of 3.7 g/L/h was finally produced, representing a relatively high level of ectoine production from renewable resources compared to other studies. This study provides valuable insights into a cost-effective and efficient method for industrial-scale ectoine production.
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Chemical Engineering Journal Advances
·
2021
·
IF 0
Xiaoge Wu,Lei Yan,Guofeng Xu,Xiaozhi Wang,JuanJuan Wang,Dionysios D. Dionysiou
The ability of high frequency ultrasonication to improve sulphate radical-based water disinfection was investigated in the laboratory. Low doses of potassium persulfate and zero-valent iron inactivated Escherichia coli (5.74 log) and Staphylococcus aureus (4.82 log) within 40 min when supported by 600 kHz ultrasound applied at 3.09 W/mL. That was far better than the removal with potassium persulfate and iron alone, since neither PS nor Fe 0 showed any killing effect on bacterial cells. Ultrasound at 3.09 W/mL was twice as effective as ultrasound at 6.81 W/mL with both bacteria. Nonlinear double Weibull and modified Hom models fitted the data well, showing an initial lag in the rate. SO 4 •ˉ made a greater contribution to the inactivation than •OH, but both induced cell lesioning, leakage and intracellular oxidation. The delivery effect mediated by 600 kHz ultrasound may promote the intracellular oxidation toward bacteria inactivation. Avoiding consumption by other competing impurities in water, further development should focus on the improvement of the utilization of SO 4 •ˉ generated for more efficient delivery for intracellular oxidation in water disinfection applications.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Aixi Tang,Xiaochao Bi,Jiayu Du,La Rao,Vasanthakumar V.,Yi-bo Hu,Ming-Lai Fu,Wenjie Sun,Baoling Yuan
The effect of polyethylene microplastics (PE-MPs) on the disinfection of Escherichia coli ( E. coli ) by sodium hypochlorite was investigated in different pH value, ionic strength , and NOM concentration to illustrate the impact of MPs on the pathogenic bacteria disinfection efficiency in nature water environment. The results showed that PE-MPs tended to agglomerate rather than disperse due to their strong hydrophobicity in water. Within 30 s, about 1.5 log 10 of E. coli was adsorbed on the surface of PE-MPs, forming subsequent protection for E. coli . Thus, the presence of PE-MPs reduced the inactivation rate of E. coli . As for the particle-free solutions, the higher solution pH, the presence of natural organic matter (NOM), and the higher concentrations of cations (monovalent Na + and divalent Ca 2+ ) were confirmed as the major influencing factors decreasing the E. coli disinfection efficiency. However, due to the adsorption and protection of PE-MPs on E. coli , the influences of complex chemistry factors on the inactivation of E. coli were reduced. The inactivation of E. coli in PE-MPs (20 NTU) solution was 1 log 10 lower than that in particle-free solution under the same water quality conditions. Therefore, considering the complex water chemistry , the existence of MPs could be a potential challenge for disinfection efficiency in the water treatment plants .
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ENVIRONMENTAL TECHNOLOGY
·
2024
·
IF 2.2
Nan He,Ziru Wang,Ling Lei,Changxuan Chen,Yixian Qin,Jingxiang Tang,Kecheng Dai,Heng Xu
Cadmium (Cd) is one of the common heavy metal pollutants in soil, which can induce various diseases and pose a serious threat to human health. Metallothioneins (MTs) are well-known for their excellent metal binding ability due to a high content of cysteine, which has great potential for heavy metal chelation. In this study, we used the Escherichia coli (E. coli) surface display system LPP-OmpA to construct a recombinant plasmid pBSD-LCF encoding LPP-OmpA-CUP1-Flag fusion protein. Then we displayed the metallothionein CUP1 from Saccharomyces cerevisiae on E. coli DH5α surface for Cd removing. The feasibility of surface display of metallothionein CUP1 in recombinant E. coli DH5α (pBSD-LCF) by Lpp-OmpA system was proved by flow cytometry and western blot analysis, and the specificity of the fusion protein in the recombinant strain was also verified. The results showed that the Cd2+ resistance capacity of DH5α (pBSD-LCF) was highly enhanced by about 200%. Fourier-transform infrared spectroscopy showed that sulfhydryl and sulfonyl groups were involved in Cd2+ binding to cell surface of DH5α (pBSD-LCF). Meanwhile, Cd removal rate by DH5α (pBSD-LCF) was promoted to 95.2%. Thus, the recombinant strain E. coli DH5α (pBSD-LCF) can effectively chelate environmental metals, and the cell surface expression of metallothionein on E. coli can provide new ideas and directions for heavy metals remediation.
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