SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2023
·
IF 4.4
Feng Lu,Xinhuan Xu,Xingdong Zhu,Linxin Shen,Weizheng Wan,Min Hu
Room temperature phosphorescent (RTP) Carbon Dots have been capturing increasing attention in recent years, while building a general method to adjust the emission color of RTP carbon dots is still a big challenge. Herein we report a simple method that combine the carbon nanodots and dyes (R6G and DCF) in SiO 2 nanosphere to get a series of multicolor RTP nanodots (CD@SiO 2 @dye) with long lifetime in aqueous solution. Leverage on chitosan quaternary ammonium as matrix and diethylenetriamine as N-doping resource to form a cross-linked skeleton as a luminescent center (namely CD), and a rigid network is formed by silica encapsulation (CD@SiO 2 ) to restrict the non-radiative transition process to generate the phosphorescence. The CD-based composites, with 1.10 s green (503 nm) phosphorescence emission, serve as activator to stimulate the corresponding luminescence of organic dyes. Then, based on Förster resonance energy transfer (FRET) process from CDs (as donor) to organic dyes (as acceptor) under UV excitation, the CD@SiO 2 @R6G emit ultra-long lifetime (1.13 s) orange-yellow (570 nm) afterglow, and CD@SiO 2 @DCF emit ultra-long lifetime (1.20 s) yellow-green afterglow (530 nm). Furthermore, it also achieves RTP colors control when the ratio of CDs and the dyes changes, the ratio of green emission and dye’s emission activated by CDs will gradually change as well. These kinds of materials keep the inherent advantages of low toxicity and luminous stability, and achieve adjustable RTP color in aqueous solution. Our research provides a strategy to synthesize water-soluble long-life RTP CDs with adjustable color and lifetime.
研究领域:
材料科学
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纳米技术
·
光化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Conglin Guo,Qu Tang,Jige Yuan,Shijie Li,Xiaoxiao Yang,Yuechen Li,Xiaobo Zhou,Haiwei Ji,Yuling Qin,Li Wu
The accurate discrimination of bacterial infection is imperative for precise clinical diagnosis and treatment. Here, this work presents a simplified sensor array utilizing “All-in-One” Pdots for efficient discrimination of diverse bacterial samples. The “All-in-One” Pdots sensor (AOPS) were synthesized using three components that exhibit fluorescence resonance energy transfer (FRET) effect, facilitating the efficient integration of multiple discrimination channels to generate specific fluorescence response patterns through a single detection under single-wavelength excitation. Additionally, machine learning techniques were employed to visually represent the fluorescence response patterns of AOPS upon exposure to bacterial metabolites derived from diverse bacterial species. The as-prepared sensor platform demonstrated excellent performance in analyzing eight common bacteria, drug-resistant strains, mixed bacterial samples, bacterial biofilms and real samples, presenting significant potential in the identification of complex samples for bacterial analysis.
研究领域:
生物传感
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医学诊断
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Gao Lu,Zhao Haimei,Zhou Xiaodan,Zhao Yuxian,Wang Yidi,Geng Huaixiao,Xu Jia,Zhang Zhichao,Han Shuang
Copper and nitrogen co-doped fluorescent carbon quantum dots (Cu-NCDs) were synthesized via a one-pot hydrothermal synthesis method using L-tyrosine and copper chloride as precursors. The as-prepared Cu-NCDs exhibited bright blue fluorescence (emission peak at 427 nm) with high photostability (10.5% intensity loss after 1 h irradiation at 365 nm). Upon introduction of tetracyclines antibiotics (TCs), include oxytetracycline (OTC), chlortetracycline (CTC) and tetracycline (TC), the fluorescence intensity of Cu-NCDs was selectively quenched through fluorescence resonance energy transfer (FRET), as confirmed by time-resolved fluorescence decay analysis. This phenomenon established a linear relationship between fluorescence quenching efficiency and TCs concentration. The calculated limits of detection (S/ N = 3) for three target analytes were determined to be 0.24 µM (OTC), 0.56 µM (CTC), and 0.19 µM (TC), respectively. Accurate quantification of TCs residues in tap water and dairy products demonstrated recovery rates of 97.94 − 112.20% (OTC), 94.93 − 110.00% (CTC), and 97.58 − 107.67% (TC), with relative standard deviations (RSDs) below 4.16% ( n = 3). Based on the paper sensor platform, on-site detection of TCs has been achieved, and visual semi quantitative analysis can be completed within 1 min.
研究领域:
分析化学
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纳米生物技术
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药物残留检测
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食品安全
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环境监测
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分子诊断
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 20
·6025-6032
·
IF 4.019
Qingju Liu,Hui Wang,Ping Han,Xiaoyuan Feng
This study reports on a fluorescent aptasensor of chlorpyrifos (CPF) based on terbium(III) based on metal–organic frameworks (Tb-MOFs) and PDDA-aggregated-gold nanoparticles (AuNPs). The dispersed AuNPs quench the emissions of the Tb-MOF, while the PDDA-aggregated AuNPs have little effect. The aptamer interacts with PDDA via electrostatic interaction to prevent the aggregation of the AuNPs, which protects the AuNPs from PDDA-induced aggregation and the fluorescence of the Tb-MOF is quenched by FRET between the Tb-MOF and the dispersed AuNPs. Upon the addition of CPF, aptamers are exhausted to form a CPF–aptamer complex, and the following PDDA causes the aggregation of AuNPs, accompanied by weak quenching of the fluorescence of the Tb-MOF. This unlabelled strategy sensitively detects CPF at a concentration of 3.8 nM with a linear range of 5–600 nM. The assay is selective for CPF over other interfering compounds. The method was successfully applied to the determination of CPF in (spiked) tap water and vegetables and fruits samples, indicating its potential applications in enlarging the target scope of luminescent-MOF-based detection for environmental and agricultural samples.
研究领域:
分析化学
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环境科学
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农业科学
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产品关联
DOI
PubMed
高影响因子
BIOCONJUGATE CHEMISTRY
·
2019
·
IF 4.349
Kun Yang,Chunlai Wang,Xiaohui Wei,Sheng Ding,Changjun Liu,Feng Tian,Fan Li
The major obstacles to the wider application of photodynamic therapy (PDT) are drawbacks of the current photosensitizers and the tissue penetration limit of the common outer light source. In the present study, the chemiluminescence (CL) from the luminol–H2O2–horseradish peroxidase reaction was explored as a potential inner light source for the intracellular activation of carbon dots (CDs)-based PDT system. To fully use the light and enhance the overall PDT yield, the nanocarrier of CDs, the light of CL, and the PDT agent chlorin e6 (Ce6) were carefully selected and designed to form an efficient and united system. Bright-yellow-emissive CDs (y-CDs) were synthesized through purposeful regulation of the absorption and emission spectra to enhance the overlapping areas in the chemiluminescence resonance energy transfer (CRET) and fluorescence resonance energy transfer (FRET) processes. Our results reflected CL-induced y-CDs-Ce6 system (10 μM) successfully generated reactive oxygen species (ROS, 35.93%), killed ∼90% SMMC-7721 cells in vitro, and significantly delayed tumor growth in vivo. On the basis of immunohistochemical observations of proliferating cell nuclear antigen (PCNA) and platelet/endothelial cell adhesion molecule-1 (PECAM-1 or CD31) results, we concluded that the CL-induced y-CDs-Ce6 system had excellent performance in cancer therapy. The enhanced therapeutic effect was ascribed to two pathways: a direct CRET process and another process of CRET with subsequent y-CD-mediated FRET (CRET-to-FRET).
研究领域:
生物医学工程
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诊疗学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 9
·3043-3055
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IF 4.478
Liang Yu-Qi,Wu Xiao-Yi,Zeng Jia-Yu,Wu Yan-Ni,Lai Jia-Ping,Sun Hui
The novel dual-emission carbon dots (DECDs) for highly selective and sensitive recognition of chlortetracycline (CTC) and cell imaging were synthesized successfully by one-step synthesis. The obtained DECDs possessed two fluorescence peaks (345 nm and 450 nm) and showed specific response to CTC, resulting in a decrease in fluorescence intensity at 345 nm, a blue shift, and an increase in fluorescence intensity at 450 nm. The obtained DECDs exhibited highly selective response to CTC and not to its analogues, such as tetracycline, doxycycline, and oxytetracycline. Thus, an excellent ratiometric probe for the detection of CTC was fabricated successfully and used for the detection of CTC in real samples with the detection limit (LOD) of 16.45 nM. More importantly, the DECDs were used for quantitative detection of CTC in living cells, which demonstrated excellent biocompatibility and broad prospects in biomedicine application. Finally, the excellent selectivity of DECDs toward CTC was attributed to the FRET mechanism and the formation of complexes. Graphical abstract
研究领域:
分析化学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2013
·Vol. 4
·期 7
·2325-2332
·
IF 5.231
Peisheng Zhang,Jian Chen,Fuhua Huang,Zhiqiang Zeng,Jia Hu,Pinggui Yi,Fang Zeng,Shuizhu Wu
Cupric pollution is a global problem, and the development of stable and sensitive fluorescent probes for cupric ions in the water phase has long been sought. In the present study, we report on the fabrication of core–shell nanoparticle-based fluorescent chemosensors for Cu2+ detection in aqueous media. The core–shell nanoparticle sensor was prepared by a facile one-pot miniemulsion polymerization, in which the fluorescent dye (4-methamino-9-allyl-1,8-naphthalimide, MANI) was covalently incorporated into particle core and the Cu2+ ligand i.e. Vinylbenzylcyclam (VBC), chemically linked onto the surface. The cyclam-functionalized fluorescent polymeric nanoparticles exhibit a high affinity for Cu2+ ions in aqueous media. Upon the addition of Cu2+, the fluorescent emission of the MANI dye in nanoparticles can be quenched on the basis of intraparticle fluorescence resonance energy transfer (FRET) from the dye in the hydrophobic PMMA core to the Cu2+–cyclam complexes on the nanoparticle surface, and the nanoparticle sensor can selectively detect the Cu2+ in water with the detection limit of 500 nM. The observed FRET efficiencies (31.6–73.4%), as well as the distance (r) between MANI (donor) and Cu2+–cyclam complexes (acceptor), were also determined. No interference was observed from other metal ions, making it a highly sensitive and selective Cu2+ probe. Moreover, the nanoparticle-based fluorescent sensor was applicable in a relatively wide pH range (pH 4–10) in water and it exhibited excellent long-term photostability for Cu2+ detection (>45 days) in aqueous media; thus, this approach may provide a new strategy for ratiometric detection of analytes in environmental and biological applications.
研究领域:
分析化学
·
环境监测
·
纳米医学
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生物传感
·
分子探针
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yanzhong Li,Sijia Qi,Chengcai Xia,Yanhao Xu,Guiyun Duan,Yanqing Ge
A novel imidazo[1,2-a]pyridine-rhodamine ratiometric fluorescent probe IP-Hg for Hg 2+ based on a fluorescence resonance energy transfer mechanism has been developed. The probe has been proved to show high sensitivity and high selectivity toward Hg 2+ . Furthermore, it could be used for imaging Hg 2+ in cells and in polluted water.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Cuiling Zhang,Caiping Ding,Guohua Zhou,Qin Xue,Yuezhong Xian
DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn 2+ ) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS 2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn 2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.
研究领域:
纳米技术
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生物传感
·
材料科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Lingling Long,Fang-Ying Wu,Pengcheng Huang
The analysis of breath acetone could be a potential non-invasive testing alternative for routine blood-based diabetic diagnosis. However, existing methods for detecting acetone are limited by some drawbacks including complex operation, low accuracy and strong interferences from other substances in exhaled breath. Herein, we presented a straightforward ratiometric fluorescence strategy for highly efficient detection of acetone in exhaled breath condensate (EBC) based on surface-confined FRET on carbon dots (CDs). In this sensing platform, the introduction of acetone triggered specific condensation reaction with abundant phenolic hydroxyl groups around the CDs; and concurrently, the in-situ generated product constituted another emitting center by absorbing the excitation energy of the CDs, which enabled visual detection according to a remarkable fluorescence color change from blue to yellowish green. Importantly, the presented probe exhibited a highly sensitive response to acetone, a good self-calibration capability, as well as excellent selectivity against common species in EBC. Finally, it was demonstrated to be applied for accurate evaluation of the acetone content in simulated diabetic EBC samples, indicating the possibility in preliminary screening and assessment of diabetes by non-invasive breath analysis.
研究领域:
分析化学
·
生物传感器
·
纳米材料
·
糖尿病研究
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Guoliang Liu,Da-Qian Feng,Yanling Qian,Wei Wang,Jun-Jie Zhu
Exploring new energy donor/acceptor pairs for constructing FRET system has important research significance. In this study, a FRET assembly by using carbon dots (CDs) as energy donor and gold nanorods (Au NRs) as energy acceptor has been proposed through covalent bond interactions by taking advantage of cysteamine as a bridge. The assembly is characterized by UV–vis absorption spectra, fluorescence spectra , FT-IR spectra and TEM images, revealing that Au NRs successfully absorbed on the surface of CDs. As for the occurrence of FRET process from CDs to Au NRs, the fluorescence signal of CDs-cysteamine-Au NRs assembly quenched significantly. Interestingly, Pb 2+ ions could bind completely with cysteamine and disturbed the FRET process, which activated fluorescence signal of the system. Based on these experimental phenomena, CDs-cysteamine-Au NRs assembly was performed as an off-on FRET biosensor for Pb 2+ ions. The linear range obtained is from 0 to 155 μM with the detection limit of 0.05 μM. Moreover, the FRET-based sensor exhibited high selectivity to the analyte Pb 2+ ions against other interference substances. Furthermore, the present approach was successfully applied to detect Pb 2+ ions in real samples, which suggested its potential applications in environmental monitoring.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Bai-Chen Liu,Bo Tu,Dong-Jin Qian
Photoluminescence regulated by the Förster resonance energy transfer (FRET) process attracts much attention for the development of novel luminescent materials and devices. Here, an efficient FRET system has been designed and constructed with the use of aggregation-induced emission (AIE) units as the energy donor and sulforhodamine B (SRB) as the acceptor in the aggregates, mixed solid powders, and Langmuir–Blodgett (LB) films. Two amphiphilic tetraphenylethene derivatives with octyl chains (C8TPEs) are synthesized and used as AIEgens, which can form well-organized mixed monolayers with arachidic acid (AA) on the SRB subphase surfaces. Efficient photoinduced energy transfer is observed from AIEgens to SRB in the C8TPE/SRB powders and C8TPE-AA/SRB hybrid LB films, with the luminescent colors changing from green through yellow to orange. The differences in their FRET behaviors are interpreted in terms of the highest occupied molecular orbital–lowest unoccupied molecular orbital (HOMO–LUMO) energy gaps, absorption spectra, and intramolecular charge transfer characteristics by time-dependent density functional theory calculations. It is further revealed that by controlling the SRB concentrations in the subphase, the luminescent emission wavelength for the C8TPE-AA/SRB hybrid LB films shifts from 490 to 592 nm, that is, from typical AIE to SRB emission. Therefore, this work provides a viable strategy for developing solid-state FRET systems by combining AIEgens’ donor with aggregation-caused quenching type acceptors, highlighting their potential in optoelectronic and sensing applications.
研究领域:
材料科学
·
光物理学
·
纳米科技
·
表面化学
·
分子光子学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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