Chemical Science
·
2024
·
IF 7.6
Yan Zhao,Weiheng Kong,Jianqing Zhu,Fengli Qu
Simultaneous implementation of tumor-associated macrophage (TAM) repolarization and immunogenic tumor cell death (ICD) elicitation enables tumor immunotherapy with high efficacy. However, the inconsistency of stimulation tolerance restricts simultaneous implementation. To address this obstacle, we validate that an H2O2-mediated regulatory strategy could achieve coordinated occurrences. To accomplish this, a bimodal responsive modulator is constructed, namely ZnO2-ATM (ATM: 3-amino-1,2,4-triazole), as an immune adjuvant to coordinate the occurrence of TAM repolarization and ICD elicitation through the endo/exogenous synergistic responsive production of H2O2. H2O2 produced by ZnO2-ATM reverses the immune-suppressive TAM from an M2 to an M1 phenotype, but induces tumor cell necrosis and promotes damage-related molecular pattern release, thereby evoking ICD. This H2O2-mediation bimodal responsive therapeutic strategy to induce the synergistic occurrence of TAM repolarization and ICD elicitation promotes effective immune effects against tumors, demonstrating that the ZnO2-ATM nanoadjuvant could be expected to provide new tools and paradigms for antitumor immunotherapy.
研究领域:
肿瘤免疫学
·
生物化学
·
药理学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yi Liang,Xiaolin Huang,Xirui Chen,Wenjing Zhang,Guo Ping,Yonghua Xiong
A novel direct competitive plasmonic enzyme-linked immunosorbent assay (dc-pELISA) was applied to detect ochratoxin A (OTA) with naked eyes. In this assay, horseradish peroxidase (HRP) + hydrogen peroxide (H 2 O 2 ) + tyramine (TYR)-induced gold nanoparticle (AuNP) aggregation was considered as a signal output; AuNP aggregation could be triggered through the phenol polymerization of TYR , which was induced by hydroxyl radicals from HRP-catalyzed H 2 O 2 ; OTA-labeled catalase (CAT) was used as a competing antigen to consume H 2 O 2 . Owing to the combined advantages of ultrahigh CAT catalytic activity for H 2 O 2 and dual-color responses (red and blue) generated through AuNP aggregation, the proposed method was highly sensitive and thus could be employed with naked eyes to detect OTA qualitatively with a cut-off limit of 150 pg/mL. Our method also demonstrated a good dynamic linear range (12.5–150 pg/mL) for quantitative OTA determination with a reliable correlation coefficient of R 2 = 0.992, a half-maximal inhibitory concentration of 84.75 pg/mL, and a detection limit of 17.8 pg/mL. In brief, this newly-designed technique is considerably suitable for high-throughput screening detection or point-of-care diagnostics in resource-constrained regions because of the easy readout of results by naked eyes without the use of advanced detection equipment.
研究领域:
食品科学
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分析化学
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生物医学工程
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DOI
高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2024
·Vol. 89
·期 3
·811-822
·
IF 2.5
Ma Yang,Ma Yongwen,Wan Jinquan,Wang Yan,Ye Gang,Zhang Zhifei,Lin Yining
View large Download slide View large Download slide Close modal Advanced oxidation technologies based on hydroxyl radical (•OH) and sulfate radical (SO 4 −• ) are two common types of advanced oxidation technologies, but there are not many reports on the application of advanced oxidation methods in actual wastewater pretreatment. This article compares the pre-treatment performance of Fe 2+ /H 2 O 2 and Fe 2+ /Persulfate systems in actual pharmaceutical wastewater, and combines EEM, GC-MS, and toxicity testing results to explore the differences in TOC, COD, and NH 3 -N removal rates, optimal catalyst dosage, applicable pH range, toxicity of effluent after reaction, and pollutant structure between the two systems. The results indicate that the Fe 2+ /H 2 O 2 system has a higher pollutant removal rate (TOC: 71.9%, COD: 66.9%, NH 3 -N: 34.1%), but also requires a higher catalyst (Fe 2+ ) concentration (6.0 g/L), and its effluent exhibits characteristic peaks of aromatic proteins. The Fe 2+ /Persulfate system has a wider pH range (pH ≈ 3-7) and is more advantageous in treating wastewater containing more cyclic organic compounds, but the effluent contains some sulfur-containing compounds. In addition, toxicity tests have shown that the toxicity reduction effect of the Fe 2+ /Persulfate system is stronger than that of the Fe 2+ /H 2 O 2 system. Two types of advanced oxidation systems were compared using actual pharmaceutical wastewater as the research object. Investigated the pretreatment effect of advanced oxidation systems and explored the possibility of applying advanced oxidation to the pretreatment process of wastewater. A deeper comparison was made between the two advanced oxidation methods by comparing the EEM and GC–MS results.
研究领域:
环境科学
·
废水处理工程
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DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-16
·
IF 10.6
Zhao Yulan,Wu Yang,Xu Quan,Liu Yi,Song Zhiyong,Han Heyou
Reactive oxygen species (ROS) has emerged as potent therapeutic agents for biofilm-associated bacterial infections. Chemodynamic therapy (CDT), involving the generation of high-energy ROS, displays great potential in the therapy of bacterial infections. However, challenges such as insufficient hydrogen peroxide (H 2 O 2 ) and over-expressed glutathione (GSH) levels within the microenvironment of bacterial biofilms severely limit the antibacterial efficacy of CDT. Herein, we have developed a multifunctional nanoplatform (CuS@CaO 2 @Dex) by integrating copper sulfide (CuS) and calcium peroxide (CaO 2 ) into dextran (Dex)-coated nanoparticles. This innovative platform enhanced ROS generation for highly efficient biofilm elimination by simultaneously supplying H 2 O 2 and depleting GSH. The Dex-coating facilitated the penetrability of CuS@CaO 2 @Dex into biofilms, while CaO 2 generated a substantial amount of H 2 O 2 in the acidic biofilm microenvironment. CuS, through a Fenton-like reaction, catalyzed the conversion of self-supplied H 2 O 2 into hydroxyl radicals (•OH) and consumed the overexpressed GSH. Additionally, the incorporation of near-infrared II (NIR II) laser irradiation enhanced the photothermal properties of CuS, improving the catalytic efficiency of the Fenton-like reaction for enhanced antibacterial effects. In vivo experiments have demonstrated that CuS@CaO 2 @Dex exhibited remarkable antibacterial and antibiofilm efficacy, exceptional wound healing capabilities, and notable biosafety. In summary, the Dex-coated nanoplatform proposed in this study, with its self-sterilization capability through ROS, holds significant potential for future biomedical applications.
研究领域:
纳米技术
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生物医学工程
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药学
·
传染病学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Yuhong Liu,Xuetao Zhang,Xinhua Shi,Xiaoping Jiang,Jing Zhang,Hui Wang,Meng Gao
Due to the important role of H 2 O 2 in human health and environment safety, the development of portable optical sensors for real-time detection of H 2 O 2 in gas phase is highly anticipated. Herein, an electrospun ratio fluorescence film (PVA@Dual-SiO 2 ) with bead structure was constructed by dual Aggregation-induced Emission (AIE) featured silica nanoparticles for convenient detection of gaseous H 2 O 2 . In this system, the blue-emitted AIE silica nanoparticles (T-SiO 2 ) with H 2 O 2 recognition capability was embedded in the spinning fiber as the fluorescent responder. Meanwhile, the red-emitted AIE silica nanoparticles (F-SiO 2 ) was designed as the fluorescent reference unit in film. By exploiting the peroxide-mediated reaction between T-SiO 2 and H 2 O 2 , the dual-emitting PVA@Dual-SiO 2 could sense gaseous H 2 O 2 with high sensitivity and selectivity by ratiometric fluorescence signals. It proved that this film could be used for detecting H 2 O 2 in food samples. This work developed a bead like fiber film sensor with dual AIE emission, which provided a representative case of the fabrication of portable and integrated film sensor with high fluorescence performance.
研究领域:
分析化学
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生物传感器
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材料科学
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环境监测
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食品安全
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产品关联
DOI
高影响因子
Catalysis Science & Technology
·
2023
·Vol. 13
·期 7
·2101-2107
·
IF 5
Jianhao Qiu,Lu Zhang,Guanglu Xia,Dingliang Dai,Yong Tang,Jianfeng Yao
Different ligand functionalized UiO-66 (UiO-66-X, X = OH, (OH)2 and NH2) were prepared and then modified by ZnIn2S4 lamellas to form ZnIn2S4/UiO-66-X heterostructures for visible-light-driven H2O2 evolution in pure water. The H2O2 yields using ZnIn2S4/UiO-66-NH2, ZnIn2S4/UiO-66-(OH)2 and ZnIn2S4/UiO-66-OH are 799, 733 and 165 μmol L−1, respectively, which are 9.5, 8.7 and 2.0 times that of ZnIn2S4/UiO-66. The high performance of ZnIn2S4/UiO-66-NH2 and ZnIn2S4/UiO-66-(OH)2 is ascribed to the benign visible-light response and Z-scheme heterostructures, and the H2O2 evolution abides by indirect O2 reduction with ˙O2− as an intermediate species. Additionally, H2O2 yields using ZnIn2S4/UiO-66-NH2 on tap water and Xuanwu Lake (Nanjing, China) water can be comparable to that on the above-deionized water. This study sheds light on the great promise of functionalized MOFs and their applications on green (photo)catalytic energy conversion.
研究领域:
材料科学
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光化学
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环境科学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Yuyan Yan,Renlu Han,Huijun Zhang,Pengxin Song,Xiangjun Wei,Yafei Hou,Jiancheng Yu,Keqi Tang
Chemodynamic therapy (CDT), which employs Fenton/Fenton-like agents to decompose hydrogen peroxide (H 2 O 2 ) into toxic hydroxyl radical (•OH) to induce cancer cell apoptosis and necrosis, holds great promise in tumor therapy due to its high selectivity . Nevertheless, its efficiency is impaired by the insufficient intracellular H 2 O 2 concentration and/or the insensitive response of Fenton/Fenton-like agents to the slightly acid tumor microenvironment (pH∼7.0–6.5). Herein, we develop a novel CDT reagent based on CeO 2 quantum dot (QD) decorated MgO 2 nanosheets engineered with cascade reactions to boost the intracellular H 2 O 2 level and high pH-activated (pH = 6.5) characteristic for an enhanced CDT. Under the tumor microenvironment (pH = 6.5), MgO 2 nanosheets that are highly reactive can react with H 2 O to produce nontoxic Mg 2+ and abundant H 2 O 2 , boosting the intracellular H 2 O 2 level. The self-generated H 2 O 2 is subsequently converted into •OH by CeO 2 QD, which is served as a relatively high pH-activated (pH = 6.5) Fenton-like agent. The sufficient intracellular H 2 O 2 supply and sensitive response to the slightly acid tumor sites significantly improve the Fenton reaction, leading to the excellent in vivo CDT results with tumor growth inhibition effect. Our work presents a distinctive paradigm for self-boosting CDT efficacy.
研究领域:
医学
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生物材料学
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肿瘤治疗学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 9
·1685
·
IF 7
Lingyu Xie,Yibin Xu,Xiaoqing Ding,Kaixuan Li,Shuang Liang,Danlei Li,Yongxia Wang,Aikun Fu,Weixiang Yu,Xiuan Zhan
Earlier studies have shown that selenomethionine (SM) supplements in broiler breeders had higher deposition in eggs, further reduced the mortality of chicken embryos, and exerted a stronger antioxidant ability in offspring than sodium selenite (SS). Since previous studies also confirmed that Se deposition in eggs was positively correlated with maternal supplementation, this study aimed to directly investigate the antioxidant activities and underlying mechanisms of SS and SM on the chicken hepatocellular carcinoma cell line (LMH). The cytotoxicity results showed that the safe concentration of SM was up to 1000 ng/mL, while SS was 100 ng/mL. In Se treatments, both SS and SM significantly elevated mRNA stability and the protein synthesis rate of glutathione peroxidase (GPx) and thioredoxin reductase (TrxR), two Se-containing antioxidant enzymes. Furthermore, SM exerted protective effects in the H2O2-induced oxidant stress model by reducing free radicals (including ROS, MDA, and NO) and elevating the activities of antioxidative enzymes, which performed better than SS. Furthermore, the results showed that cotreatment with SM significantly induced apoptosis induced by H2O2on elevating the content of Bcl-2 and decreasing caspase-3. Moreover, investigations of the mechanism revealed that SM might exert antioxidant effects on H2O2-induced LMHs by activating the Nrf2 pathway and enhancing the activities of major antioxidant selenoenzymes downstream. These findings provide evidence for the effectiveness of SM on ameliorating H2O2-induced oxidative impairment and suggest SM has the potential to be used in the prevention or adjuvant treatment of oxidative-related impairment in poultry feeds.Keywords:selenomethionine;sodium selenite;antioxidants;glutathione peroxidase;Nrf2
研究领域:
动物科学
·
生物化学
·
细胞生物学
·
营养学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Yijing Ai,Fan Shi,Xiao Han,Xiaoqing Li,Wanting Fu,Baoli Wang,Xiaoping Zhang,Wei Sun
In this work, a simple one-step pyrolysis process was designed to synthesize Fe-doped graphite carbon nitride (Fe–C 3 N 4 ) nanosheets with FeCl 3 as Fe source. The resulted samples were characterized by various characterization techniques, and the results showed that Fe species might form Fe–N bonds with N atoms of C 3 N 4 , which was similar to the iron (Ⅲ) porphyrin active center of horseradish peroxidase. Then Fe–C 3 N 4 nanosheet was further mixed with carbon nanotube (CNT) to obtain Fe–C 3 N 4 @CNT nanocomposite, which was further modified on the self-made flexible printed electrode (PE) to construct the flexible working electrode. Fe–C 3 N 4 nanosheets show good catalytic activity for H 2 O 2 and the introduction of CNT can effectively improve the electron transfer efficiency at the modified electrode interface. By combining with wireless portable electrochemical workstation, the sensor shows a wider detection range (10.0 μmol/L–20.0 mmol/L) and a lower limit of detection (0.38 μmol/L, S/N = 3) for the H 2 O 2 analysis. Furthermore, the sensor can be applied to the real time and in-situ analysis of H 2 O 2 secreted from live cells. In word, this self-supported sensing electrode shows great potential applications in monitoring the pathological and physiological dynamics of cancer cells.
研究领域:
材料科学
·
电化学
·
生物传感器
·
纳米技术
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2024
·Vol. 14
·期 12
·886
·
IF 3.8
Zhiyong Fang,Xiangyu Song
In the study presented herein, a series of TiO2-supported Ru catalysts were prepared through over-impregnation with different amounts of H2O2and applied to the catalytic combustion of dichloromethane (DCM). The experimental results show that the optimal (1 H2O2)-Ru@TiO2catalyst sample yields 90% DCM conversion at 301 °C, which is 40 °C lower than the temperature required by the (0 H2O2)-Ru@TiO2catalyst. This good activity is related to its high number of acid sites (especially Brønsted acid sites), an appropriate amount of uniformly dispersed RuO2particles, and strong interaction between RuO2and the TiO2support. In addition, excessive H2O2leads to the growth and phase segregation of RuO2, which weakens the interaction between RuO2and the TiO2support and decreases catalytic activity. Moreover, H2O2addition also contributes to the stability of catalysts, which possibly results from the re-dispersion of RuO2on the TiO2support during the reaction.
研究领域:
环境科学
·
催化化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Kai Yin,Lin Deng,Jinming Luo,John Crittenden,Chengbin Liu,Yuanfeng Wei,Longlu Wang
Phenicol antibiotics (PABs) degradation by UV/H 2 O 2 is important because we need to determine the reduction in toxicity and disinfection byproducts for post-chlorine. In this study, the degradation of PABs, including florfenicol (FLO), chloramphenicol (CAP) and thiamphenicol (THA), was examined. The pseudo-first order degradation rate constants of PABs were 3 times higher in ultrapure water (UW) than that in synthetic wastewater (SW) for these conditions: [PABs] 0 = 1 μM, [H 2 O 2 ] = 0.1 mM, and I 0 = 1.985 × 10 −6 E L −1 s −1 . Fulvic acid (FA) and HCO 3 – inhibited PABs degradation, Cl − and NO 3 – concentrations of up to 5 mM and 10 mM had a negligible impact. The impact of water matrix on PABs degradation was successfully predicted using pseudo-steady-state kinetic model. The degradation of PABs was triggered via hydroxylation and/or hydrogen abstraction. The treatment of PABs via UV/H 2 O 2 could decrease their antimicrobial properties, while the byproducts of FLO and THA showed higher acute toxicity in Vibrio fischeri . In addition, two identification products (TP-276 and TP-354) of FLO had higher ecotoxicity toxicity (using ECOSAR) in fish, daphnid and green algae. The trichloromethane formation potential (TCMFP) for PABs with post-chlorination in UW and SW can be reduced after UV/H 2 O 2 compared to UV, and is related to the corresponding decrease of dissolved organic carbon (DOC).
研究领域:
环境科学
·
毒理学
·
水处理工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Zijian Song,Ben Wang,Wu Yang,Tao Chen,Chuan Ma,Lushi Sun
Fe 2.5 M 0.5 O 4 (M = Mn, Ti and Cu) were investigated for the first time as the catalyst for simultaneous removal of NO and SO 2 through catalytic oxidation-absorption with vaporized H 2 O 2 . The physicochemical properties of as-prepared catalysts were comprehensively characterized by XRD, SEM, TEM, BET, XPS, TPR, VSM, TG and PL analyses. Activity test results showed that SO 2 can be completely removed under most conditions. However, NO removal was affected by catalyst, H 2 O 2 consumption, reaction temperature, GHSV and coexistence gases (i.e., H 2 O, O 2 , NO and SO 2 ). Mn, Ti and Cu doping in the magnetite can remarkably enhance NO removal. Redox pairs and oxygen vacancy of catalysts were considered as vital factors affecting OH generation. Base on the catalyst characterization and DFT calculation results, the main mechanism of Mn, Ti and Cu doping on the catalytic activity improvement was explained as follows: (a) Mn, Ti and Cu doping can increase the catalyst surface area; (b) Ti on the surface can directly promote the catalytic decomposition of H 2 O 2 ; and (c) Mn and Cu doping can promote the generation of oxygen vacancy and indirectly increase the activity. Moreover, two arrangement forms of an industrial simultaneous removal system for NO, SO 2 and Hg 0 were proposed.
研究领域:
环境科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）