ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
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2023
·
IF 3.9
Yingying Chen,Wei Song,He Zhang,Xinping Ji
Epithelial-mesenchymal transition (EMT) of alveolar epithelial cells is a vital process in idiopathic pulmonary fibrosis (IPF), which results in the accumulation of fibroblasts and myofibroblasts and excessive extracellular matrix deposition. Based on RNA sequencing analysis and GEO dataset reanalysis, we screened out MICALL2, a gene upregulated in the lungs of IPF mice and alveolar epithelial type II (ATII) cells from IPF patients, and aimed to explore its role in IPF. We validated the expression of MICALL2 in bleomycin (BLM)-induced IPF mice and TGF-β1-stimulated ATII cells (primary murine ATII cells and A549 cells), and explored the role of MICALL2 in IPF by knockdown of MICALL2 in BLM-induced mice and TGF-β1-stimulated ATII cells. We found that MICALL2 was upregulated in the lungs of BLM-induced mice and TGF-β1-stimulated ATII cells. MICALL2-deficient mice had reduced fibrogenesis and restrained EMT upon BLM challenge. Knockdown of MICALL2 restrained the EMT process, in vitro, through impeding β‐catenin nuclear translocation. Mechanistically, we demonstrated that NPAS2 is directly bound to the promoter of MICALL2. Altogether, our data revealed transactivation of MICALL2 induced by NPAS2, contributing to activation of the Wnt/β-catenin pathway in ATII cells, thus leading to the EMT process and subsequent pulmonary fibrosis. Interfering with MICALL2 may represent an innovative therapeutic target to mitigate the extent of IPF.
研究领域:
肺科学
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细胞生物学
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分子生物学
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病理学
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DOI
PubMed
高影响因子
CHEMISTRY OF MATERIALS
·
2015
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IF 8.354
Xinmiao Liang,Liying Wang,Yangming Jiang,Jian Wang,Huan Luo,Chaoyang Liu,Jiwen Feng
Li10GeP2S12 (LGPS) is a new solid electrolyte of the highest Li ionic conductivity reported as of now. An anisotropic 3D Li ionic transport network consisting of an ultrafast Li 1D diffusion tunnel and fast in-plane 2D pathways was previously predicted by molecular dynamics simulations. In this paper, we have studied in detail the Li ion dynamics in LGPS by multiple solid-state NMR methods. Two different Li motion processes, characterized by apparently different activation energies of 0.16 eV and 0.26 eV, were unambiguously probed by both 7Li and 31P solid-state NMR and assigned to Li ion diffusions in the 1D tunnel and in the 2D plane, respectively. 31P spin-locking relaxation measurement further reveals that interstitial position Li(4) is active for in-plane Li migration.
研究领域:
材料科学
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物理化学
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DOI
高影响因子
Journal of Physical Chemistry C
·
2024
·
IF 3.3
Cong Fu,Lingfang Liu,Cici Wang,Weixin Huang,Guofeng Zhao
Unraveling the effect of defects on the concentration of surface-available photoexcited charges is of great importance for semiconductors in photocatalysis. Herein, by using in situ diffuse-reflectance infrared (IR) spectroscopy, the concentration of surface-available photoholes on TiO2 nanoparticles with tunable bulk/surface defects was quantified by an adsorbate (methanol) surface elementary reaction kinetic analysis method. Reaction kinetics showed that the oxidation of the adsorbed methanol species under light irradiation was a first-order reaction. Therefore, the concentration of surface-available photoholes can be calculated from the rate constants of adsorbed methanol on the TiO2 surface. By carefully comparing the specific reaction rate constants on the TiO2 surface with various bulk/surface defect ratios, we demonstrated for the first time that increasing the bulk defect concentration relative to the surface defects decreases the concentration of surface-available photoholes. This trend implicates different effects of bulk and surface defects on band bending and subsequent interfacial hole transfer processes in TiO2 nanoparticles. These insights not only reveal the mechanistic role of surface and bulk defects in photocatalysis but also highlight that in situ IR spectroscopy combined with reaction kinetic analysis has enormous potential in elucidating photocatalytic mechanisms.
研究领域:
光催化
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材料化学
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表面科学
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物理化学
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纳米材料
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分析化学
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DOI
高影响因子
Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 8
·2676-2681
·
IF 7.059
Yanxun Li,Xianglang Sun,Xuning Zhang,Dongyang Zhang,Haoran Xia,Jiyu Zhou,Nafees Ahmad,Xuanye Leng,Shuo Yang,Yuan Zhang,Zhong'an Li,Huiqiong Zhou
Herein, a new electropolymerized (EP) film named poly-triphenylcarbazole fluoranthene (p-TPCF) has been in situ synthesized by an electrochemical cyclic voltammetry method and applied to tune the work-function (WF) of the PEDOT:PSS hole-transporting layer (HTL) in organic solar cells (OSCs). Multiple techniques, including Kelvin probe force microscopy (KPFM), light intensity dependent solar cell characterization, Mott–Schottky analysis, and transient photovoltage (TPV) and transient photocurrent (TPC) measurements, have been explored to provide insights into the mechanism. Benefiting from the deeper work-function (WF), larger built-in voltage (Vbi), decreased recombination, and longer carrier lifetime, the devices with EP films exhibited superior device performance with simultaneously enhanced Voc, Jsc and FF.
研究领域:
有机光伏
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材料科学
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电化学
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聚合物科学
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器件物理
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DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2026
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IF 4.9
Ping Wang,Yue Wang,Changjiang Li,Zhongcheng Ke,Yan Gong,Boyuan Liu,Meng Wang,Xiangqun Jin
Disulfiram (DSF), an FDA-approved anti-alcoholism drug, has demonstrated potent anticancer activity against peritoneal carcinomatosis. However, its therapeutic potential is severely hampered by poor aqueous solubility and instability. Nanoemulsion system provides a promising platform to overcome these challenges. Herein, this study developed and optimized a DSF-loaded nanoemulsions (DSF-NE) to enhance the therapeutic efficacy of DSF in peritoneal carcinomatosis. The optimized DSF-NE exhibited several formulation advantages, including a small particle size (∼84 nm), narrow size distribution (∼0.1), high encapsulation efficiency (>90 %) and sustained-release behavior. Subsequently, cytotoxicity assays against MGC-803, SKOV-3 and Caco-2 cells revealed that DSF-NE significantly enhanced cytotoxic effects compared to DSF, with IC 50 values reduced by 74 %, 62 % and 56 %, respectively. Consistently, DSF-NE achieved a tumor inhibition rate 108.57 % higher than that of DSF in a murine peritoneal carcinomatosis model. The main mechanism involves enhanced cellular uptake of DSF-NE, which promotes ROS generation and subsequently triggers apoptosis. Collectively, these findings highlight DSF-NE as a nanoformulation strategy that not only improves the therapeutic performance of DSF in peritoneal carcinomatosis but also offers broader implications in the development of effective delivery systems for poorly soluble and unstable drugs.
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产品关联
DOI
高影响因子
NEUROCHEMICAL RESEARCH
·
2026
·Vol. 51
·期 4
·207
·
IF 4.5
Fu Jie,Zhang Shaotao,Chu Yifei,Li Jinglun,Chen Xiu
Microglia-mediated neuroinflammation is closely associated with the pathogenesis of epilepsy. Mammalian sterile-20-like kinase 4 (MST4) has been suggested a regulator of inflammation. However, the effect of MST4 on microglia neuroinflammation in epilepsy remains unclear. A pilocarpine-induced rat epilepsy model was constructed and a lipopolysaccharide (LPS)-stimulated microglia cell model was applied in the current research. Knockdown or overexpression of MST4 was established using lentivirus transfection. Electroencephalograph (EEG) was employed to measure brain activities of rats. The protein and mRNA expressions were detected using western blot and qRT-PCR, respectively. Immunofluorescent staining was conducted to detect the distribution of the proteins. TUNEL staining was performed to evaluate cell apoptosis. The protein interaction was evaluated with Co-IP assay. Our results showed that MST4 and nuclear PKM2 expressions were increased in epileptic rats compared to control and colocalized with microglia. MST4 overexpression inhibited microglia activation, the release of TNF-α and IL-1β, and improved neuronal apoptosis in epileptic rats. Furthermore, MST4 interacted with PKM2 and regulated PKM2 nuclear translocation. Inhibiting PKM2 nuclear translocation by TEPP-46 reversed the promoting effect of MST4 knockdown on microglia neuroinflammation. In summary, our study demonstrated that MST4 alleviated microglia-mediated neuroinflammation in epilepsy, and the mechanism of MST4-mediated anti-neuroinflammatory effects may be associated with the inhibition of PKM2 nuclear translocation.
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产品关联
DOI
PubMed
高影响因子
DRUG METABOLISM AND DISPOSITION
·
2025
·
IF 4
Dafa Yi,Jiazong Ye,Zebei Lu,Leilei Lu,Tengfei Qi,Tong Luo,Xiuyun Peng,Abdullah Al Mamun,Quan Zhou,Daqing Chen,Shuanghu Wang
This study investigates the potential drug-drug interactions between commonly prescribed anti-hypertensive drugs and mobocertinib in clinical practice to provide the scientific basis for the appropriate therapeutic application of drugs. The study used UPLC-MS/MS to detect the essential metabolites of mobocertinib in rat liver microsomes. Moreover, several anti-hypertensive drugs were used as inhibitors and the IC 50 values were determined for calcium channel blockers, angiotensin II receptor blockers and α and β receptor blockers against mobocertinib. The inhibitory mechanism of mobocertinib was further investigated for nicardipine, irbesartan, telmisartan, carvedilol, prazosin hydrochloride and spironolactone. Twenty-five Sprague Dawley ( SD) rats were randomly divided into five groups: benidipine, nicardipine, telmisartan, irbesartan and blank control groups. The drugs were administered over a period of 7 days. Mobocertinib was administered once by gavage to five groups and then blood samples were collected for further analysis. UPLC-MS/MS was performed to analyze the concentration of mobocertinib and its metabolites in rat plasma. Most anti-hypertensive drugs were found to inhibit the metabolism of mobocertinib in vitro. Several anti-hypertensive drugs were also found to increase the plasma concentration and decrease the metabolic rate of mobocertinib when administered multiple times. More importantly, we found that benidipine, nicardipine, telmisartan and irbesartan inhibited the metabolism of mobocertinib in vitro and in vivo . In summary, our data reveal that anti-hypertensive drugs interact with mobocertinib and additional attention is needed when co-administering the two drugs.
研究领域:
药理学
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临床医学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
·
2024
·
IF 5.5
Jinbin Cui,Gang Zhao,Wei Xie,Yang Yang,Xing Fu,Hezhang Meng,He Liu,Mengfei Tan,Dandan Chen,Chao Rong,Yangyun Wang,Yong Wang,Leshuai W. Zhang
Copper sulfide nanoparticles (NPs) synthesized through biomineralization have significant commercial potential as photothermal agents, while the safety evaluation of these NPs, especially for patients with non-alcoholic fatty liver (NAFL), remains insufficient. To explore the differential hepatotoxicity of copper sulfide NPs in NAFL conditions, we synthesized large-sized (LNPs, 15.1 nm) and small-sized (SNPs, 3.5 nm) BSA@Cu 2-x S NPs. A NAFL rat model fed with high fat diet (HFD) was successfully established for a 14-day subacute toxicity study by daily repeated administration of BSA@Cu 2-x S NPs. Our findings from serum biochemistry and histopathological examinations revealed that copper sulfide at both sizes NPs induced more pronounced liver damage in NAFL rats than rats fed with normal diet. Transcriptome sequencing analysis showed that LNPs activated inflammation and DNA damage repair pathways in the livers of NAFL rats, while SNPs displayed minimal inflammation. A three-dimensional spheroid model of NAFL developed in our in-house cell spheroid culture honeycomb chips demonstrated that LNPs, but not SNPs, triggered a distinct release of inflammatory factors and increased reactive oxygen species through Kupffer cells. These results highlight that NAFL condition exacerbated the hepatotoxicity of BSA@Cu 2-x S NPs, with SNPs emerging as safer photothermal agents compared to LNPs, suggesting superior potential for clinical applications.
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·
IF 3.2
Liyang Wei,Qian Hu,Lei He,Guoping Li,Jiukai Zhang,Ying Chen
The folk proverb “the older, the better” is usually used to describe the quality of Citrus grandis “Tomentosa” (CGT) in China. In this study, CGT aged for 6-, 12-, 16-, and 19-years were collected for the investigation of infusion color, main bioactive components, antioxidant activity, metabolic composition, and pathway. The results found that infusion color, the total phenolic and flavonoid, and antioxidant activity of CGT were obviously changed by aging process. Through untargeted metabolomics, 55 critical metabolites were identified to in discrimination of CGT with different storage ages, mainly including phenylpropanoids, lipids, and organic oxygen compounds. Twenty compounds that showed good linear relationships with storage ages could be used for year prediction of CGT. Kyoto encyclopedia of genes and genomes enrichment pathway analysis uncovered important metabolic pathways related to the accumulation of naringin, kaempferol, and choline as well as the degradation of benzenoids, thus supporting that aged CGT might be more beneficial to health. Correlation analysis provided that some key metabolites with bitter taste and biological activity were involved in the darkening and reddening of CGT infusion during aging, and total phenolic and flavonoid were more strongly associated with the antioxidant activity of CGT. This study systematically revealed the quality changes and key metabolic pathways during CGT aging at first time. Practical Application This study reveals the differences in quality attributes and metabolic profile between CGT with different storage ages, providing guidance for consumers' consumption, and also providing more scientific basis for the quality evaluation and improvement of CGT.
研究领域:
食品科学
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代谢组学
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药理学
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植物科学
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产品关联
DOI
PubMed
高影响因子
Environmental Science-Water Research & Technology
·
2024
·
IF 3.5
Fan Bai,Zongping Wang,Guodong Wan,Lisan Cao,Yujie Cheng,Zhenbin Chen,Pengchao Xie
In this study, Fe(III)/sulfite (S(IV)) system was used to treat two actual water samples including a lake water sample and a secondary sedimentation tank effluent sample containing tribromophenol (TBP) with combined pre-oxidation and coagulation process. This process not only efficiently removed TBP but also facilitated the removal of background organic compounds. The treatment efficiency increased with the increased S(IV) addition times. The results from scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) showed that the in-situ generated flocs in the Fe(III)/S(IV) system exhibited enhanced specific surface area, adsorption capacity, and coagulation properties compared to those produced in single Fe(III) coagulation, with generating more Fe3O4 particles. The treatment efficiencies of TBP and background organic materials initially rose and then fell with an increase in S(IV) dosage. Moderately incrementing Fe(III) dosage also boosted the removal of TBP and background organic substances, whereas excessive Fe(III) dosage provided limited additional benefits. In the selected pH range of 3-8, good removal of turbidity could be always achieved, and the best removal of TBP and background organic substances was found at pH 4. Overall, this study indicated that the Fe(III)/S(IV) system with combined pre-oxidation and coagulation process would be an effective treatment strategy for aquatic organic micropollutants.
研究领域:
环境科学
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水处理技术
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产品关联
DOI
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 46
·7955-7964
·
IF 2.7
Si-si Liu,Shi-bao Chen,Yu-bo Yue,Xiao-hao Li,Chi Zhang,Guang-Guo Ying,Chang-Er Chen
Due to their wide applications, occurrence and “PFAS-like” environmental behaviors, ionic liquids (ILs) represent a new challenge for the environmental monitoring community, who require robust analytical methods that can determine accurately and efficiently their environmentally relevant concentrations. A new passive sampling method based on the diffusive gradients in thin films (DGT) technique was developed for the measurement of imidazole-based ILs in waters using a mixed-mode cation exchange (MCX) resin as the adsorbent. The selected binding gel had a high binding capacity (>170 μg per disc) for ILs. Diffusion coefficients measured using a diffusion cell correlated well with alkyl chain lengths (r2 = 0.95) and retention times (r2 = 0.88), providing a simple and rapid prediction approach for other ILs. The assembled MCX-DGT sampler exhibited a linear accumulation for at least 120 h. MCX-DGT also showed good performance under typical freshwater conditions (pH 5–8, ionic strength 0.001–0.01 M, and humic acid 0–5 mg L−1), while still being problematic for aquatic conditions with higher ionic strength (>0.1 M) or DOM (>10 mg L−1). Laboratory deployment (for up to 3 days) in spiked natural freshwater (SNW) resulted in linear mass uptakes for the short-chain ILs (C2–C8), and their DGT-measured concentrations agreed well with solution concentrations. However, MCX-DGT significantly overestimated the concentrations of the long-chain ILs (C10–C12) when deployed in SNW for one day or more, which is attributed to the strong competitive adsorption of the long-chain ILs by natural organic matter. In situ field evaluation along with grab sampling found no target ILs in a wastewater treatment plant and its receiving river, implying that these new chemicals might not be widely used in South China now. This is the first report on the DGT technique for ILs and might provide an effective tool for monitoring short chain length ILs in the aquatic environment in the near future.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-12
·
IF 5.3
Huang Yang,Wu Shangming,Yang Siyi,He Miao,Ma Wenhao,Fa Huanbao,Wang Yongzhong,Hou Changjun,Yang Mei
Dopamine (DA) is a key neurotransmitter whose concentration affects various neurological disorders. Unlike previous methods that synthesize non-fluorescent polydopamine (NFL-PDA) under alkaline conditions, this study introduces a novel “turn-off” sensing method for DA using NFL-PDA synthesized through a unique reaction pathway. In our approach, CuO 2 nanodots, created via a simple reduction method, catalyze the formation of hydroxyl radicals (•OH) in acidic conditions, triggering the oxidative polymerization of DA into NFL-PDA. The reaction between DA and CuO 2 nanodots in acidic solution was examined to understand the process. UiO-66-NH 2 was then used to test NFL-PDA’s fluorescence quenching ability and to further investigate the DA determination mechanism. Results showed that fluorescence quenching was due to enhanced non-radiative energy transfer and Förster resonance energy transfer (FRET) between NFL-PDA and UiO-66-NH 2 . This led to the development of a simple “turn-off” fluorometric DA determination method with a linear range of 0.1–200 μmol/L and a limit of detection (LOD) of 0.0575 μmol/L. Although this method does not outperform existing NFL-PDA synthesis methods under alkaline conditions, it provides a new synthesis approach and application for sensing DA, contributing to the basic theory of chemical sensing. Additionally, DA determination in human serum samples was successfully achieved, with results consistent with high-performance liquid chromatography (HPLC). Graphical abstract
研究领域:
化学传感
·
神经科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）