ALTERNATIVE THERAPIES IN HEALTH AND MEDICINE
·
2024
·
IF 1.9
Chunshan Wei,Zhulin Wu,Xiaoya Yang,Xiaoyan Jiang,Lianan Wang,Jinsong He,Guangdong Tong
Background: In China, traditional Chinese medicine (TCM) is an important part of the comprehensive treatment of hepatocellular carcinoma (HCC), and Chinese herb formulas with the effect of "yiqi jianpi jiedu huayu" (replenishing qi, strengthening spleen, and removing toxicity and blood stasis) are the common and efficient treatments for HCC. However, the mechanism of these formulas in treating HCC remain unclear. Objective: In this paper, our goal is to explore the potential mechanism of Phyllanthus urinaria L anti-neoplastic decoction (PAD), the representative formula of "yiqi jianpi jiedu huayu", in treating HCC. Design: The research team performed the network pharmacology and in vitro experiment (preparation of PAD aqueous extract, cell cultures and MTT assay, cell apoptosis assay, wound healing assay, transwell assays, western blot). Setting: The study took place in the Department of Hepatology, the Fourth Clinical Medical College of Guangzhou University of Chinese Medicine (Shenzhen Traditional Chinese Medicine Hospital), China. Outcome measures: The active components and targets of PAD and HCC targets were screened by five Chinese herbs and two disease databases respectively. The network pharmacology was utilized to construct the relationship network between PAD and HCC, and the mechanism was predicted by pathway enrichment analysis. The experiment was performed to verify the intervention effect of PAD on HCC and phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) pathway. Results: The relationship network between PAD and HCC suggested that PAD mainly regulated the potential therapeutic targets of HCC by key active components such as quercetin, luteolin, calycosin, wogonin, and pinocembrin. Pathway analysis demonstrated PAD could play an anti-HCC effect via multiple pathways (e.g., PI3K/Akt). Results of the experiment showed that PAD could effectively inhibit the proliferation and migration of HCC cells, and promote HCC cells apoptosis in a concentration-dependent behavior. Additionally, PAD could decrease the protein expression of phosphorylated PI3K/Akt. Conclusion: PAD mainly exerts an anti-HCC effect through multiple active components represented by quercetin and multiple pathways represented by the PI3K/Akt pathway. This study provided an experimental basis for the clinical application of PAD.
研究领域:
肿瘤学
·
中医药学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 47
·11235-11250
·
IF 7
Chan Chen,Haisheng Ren,Weikang Tang,Mengqi Han,Qinfei Chen,Hong Zhou,Jiadong Chen,Yuyue Gao,Wenbin Liu
L-3,4-Dihydroxyphenylalanine (L-DOPA) is widely used in Parkinson's disease treatment and is therefore in high demand. Development of an efficient method for the production of L-DOPA is urgently required. Nanozymes emulating tyrosine hydroxylase have attracted enormous attention for biomimetic synthesis of L-DOPA, but suffered from heterogeneity. Herein, a spherical porous iron–nitrogen–carbon nanozyme was developed for production of L-DOPA. Tannic acid chelated with ferrous ions to form a tannin-iron coordination framework as a carbon precursor. Iron and nitrogen co-doped carbon nanospheres were assembled via an evaporation-induced self-assembly process using urea as a nitrogen source, F127 as a soft template, and formaldehyde as a crosslinker. The nanozyme was obtained after carbonization and acid etching. The nanozyme possessed a dispersive iron atom anchored in the Fe–N coordination structure as an active site to mimic the active center of tyrosine hydroxylase. The material showed spherical morphology, uniform size, high specific surface area, a mesoporous structure and easy magnetic separation. The structural properties could promote the density and accessibility of active sites and facilitate mass transport and electron transfer. The nanozyme exhibited high activity to catalyze the hydroxylation of tyrosine to L-DOPA as tyrosine hydroxylase in the presence of ascorbic acid and hydrogen peroxide. The titer of DOPA reached 1.2 mM. The nanozyme showed good reusability and comparable enzyme kinetics to tyrosine hydroxylase with a Michaelis–Menten constant of 2.3 mM. The major active species was the hydroxyl radical. Biomimetic simulation of tyrosine hydroxylase using a nanozyme with a fine structure provided a new route for the efficient production of L-DOPA.
研究领域:
生物化学
·
材料科学
·
医学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 10
·6063-6075
·
IF 4.036
Gao Lidi,Hu Xingfang,Qin Shili,Chu Hongtao,Zhao Xuan,Wang Bingbing
A new kind of chiral zirconium based metal–organic framework, L-Cys-PCN-222, was synthesized using L-cysteine (L-Cys) as a chiral modifier by a solvent-assisted ligand incorporation approach and utilized as the chiral stationary phase in the capillary electrochromatography system. L-Cys-PCN-222 was characterized by X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, Fourier-transform infrared spectra, nitrogen adsorption/desorption, circular dichroism spectrum, zeta-potential and so on. The results revealed that L-Cys-PCN-222 had the advantages of good crystallinity, high specific surface area (1818 m2 g−1), thermal stability and chiral recognition performance. Meanwhile, the L-Cys-PCN-222-bonded open-tubular column was prepared using L-Cys-PCN-222 particles as the solid phase by ‘thiol–ene’ click chemistry reaction and characterized by scanning electron microscopy, which proved the successful bonding of L-Cys-PCN-222 to the column inner wall. Finally, the stability, reproducibility and chiral separation performance of the L-Cys-PCN-222-bonded OT column were measured. Relative standard deviations (RSD) of the column efficiencies for run-to-run, day-to-day, column-to-column and runs were 1.39–6.62%, and did not obviously change after 200 runs. The enantiomeric separation of 17 kinds of chiral compounds including acidic, neutral and basic amino acids, imidazolinone and aryloxyphenoxypropionic pesticides, and fluoroquinolones were achieved in the L-Cys-PCN-222-bonded OT column. These results demonstrated that the chiral separation system of the chiral metal–organic frameworks (CMOFs) coupled with capillary electrochromatography has good application prospects.
研究领域:
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
Cell Reports Methods
·
2024
·
IF 4.3
Jinli Geng,Yaxiong Yang,Boying Li,Zhen Yu,Shuang Qiu,Wen Zhang,Shixin Gao,Nan Liu,Yi Liu,Bo Wang,Yubo Fan,Chengfen Xing,Xiaodong Liu
Genetically encoded inhibitors of Ca V 1 channels that operate via C-terminus-mediated inhibition (CMI) have been actively pursued. Here, we advance the design of CMI peptides by proposing a membrane-anchoring tag that is sufficient to link the inhibitory modules to the target channel as well as chemical and optogenetic modes of system control. We designed and implemented the constitutive and inducible CMI modules with appropriate dynamic ranges for the short and long variants of Ca V 1.3, both naturally occurring in neurons. Upon optical (near-infrared-responsive nanoparticles) and/or chemical (rapamycin) induction of FRB/FKBP binding, the designed peptides translocated onto the membrane via FRB-Ras, where the physical linkage requirement for CMI could be satisfied. The peptides robustly produced acute, potent, and specific inhibitions on both recombinant and neuronal Ca V 1 activities, including Ca 2+ influx-neuritogenesis coupling. Validated through opto-chemogenetic induction, this prototype demonstrates Ca 2+ channel modulation via membrane-assisted molecular linkage, promising broad applicability to diverse membrane proteins.
研究领域:
神经科学
·
分子生物学
·
生物工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Qinghao Liu,Juhong Wu,Maosen Chen,Jie Zhong,Jianzhong Huang,Bingmei Wang,Jinyu Li,Zhibin Zhao,Feng Qi
l-Tryptophan decarboxylase (TDC) and l-3,4-dihydroxyphenylalanine decarboxylase (DDC) catalyze the decarboxylation of l-tryptophan, 5-hydroxytryptophan, and l-3,4-dihydroxyphenylalanine. In this study, we analyzed the amino acid compositions of the substrate-binding pockets of TDC from Catharanthus roseus (CrTDC) and DDC from Sus scrofa (SsDDC), explored the specificity of key amino acids within these pockets, and elucidated mechanisms influencing substrate selectivity and catalytic activity in both enzymes, using whole-cell catalysis to screen mutants and determine enzymatic kinetic parameters. The results demonstrated that residues Ala-103 and Val-122 in CrTDC, along with their corresponding sites Thr-82 and Ile-101 in SsDDC, significantly influence substrate selectivity and catalytic efficiency. Molecular dynamics simulations revealed that substrate selectivity and catalytic efficiency depends on the nucleophilic attack distance between the substrate’s amino group and the C4′ of pyridoxal 5′-phosphate. This study elucidates the catalytic mechanisms and structural bases of TDC and DDC, guiding enhancements in the related aromatic monoamine biosynthesis.
研究领域:
生物化学
·
分子生物学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Yonghong Wang,Zhuo Jin,Xinru Zhang,Jinping Li
The incorporation of lamellar materials into polymer matrix can improve the gas separation performance of mixed matrix membranes (MMMs), which have great potential in chemical separation process. However, few intrinsic selectivity sites on MoS 2 sheets suppress further employment in the membrane separation. In this work, the vacancies and defects-containing MoS 2 sheets were prepared by high temperature oxidation and hydrothermal reaction in NaOH solution, and then L-cysteine-functionalized MoS 2 (Cys-MoS 2 ) was synthesized by a thiol-click chemistry reaction between S vacancies and thiol groups as confirmed by transmission electron microscope (TEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and N 2 absorption. After blending with polyether-block-amide (Pebax), the as-prepared MMMs showed outstanding mechanical properties as a result of the homogeneous dispersion of Cys-MoS 2 and hydrogen bonding between two phases as evidenced by attenuated total reflectance-FTIR, scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The highest permeability of 297 Barrer accompanied with a CO 2 /N 2 selectivity of 120 was obtained for MMMs loaded with 1.5 wt% Cys-MoS 2 , which were enhanced by 206.2 % and 124.7 % over the pristine Pebax membrane, respectively. Meanwhile, this value was superior to that of the previously reported Pebax-based MMMs, transcending 2019 upper bound. The enhanced gas separation performance is due to the improved CO 2 adsorption selectivity by the vacancies and defects of Cys-MoS 2 , while the amine groups carrier enhanced CO 2 reactivity selectivity.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Dai Lu,Xing Tian,Zixuan Fu,Sizhao Yang,Yixing Qiu,Yupei Yang,Bin Li,Wei Wang
Despite extensive research into nanozymes, developing highly active catalysts with broad application potential remains a significant challenge. In this study, we synthesized hollow FeNi Prussian blue analog (H-FeNi PBA) nanocages through a straightforward chemical etching method, using FeNi PBA nanocubes as precursors. The resulting H-FeNi PBA nanocages exhibited enhanced peroxidase-mimetic activity, attributed to their increased specific surface area, pore volume, and Fe 2+ content. Leveraging this activity, we developed a novel colorimetric sensing platform for the detection of mercury ions (Hg 2+ ) and L-cysteine (Cys). The platform operates based on the peroxidase-mimetic activity of H-FeNi PBA nanocages, the inhibitory effect of Cys on this activity, and the restoration of activity through the specific complexation between Cys and Hg 2+ . After optimization, the platform demonstrated wide linear detection ranges (0.1–42 μM for Hg 2+ and 1–64 μM for Cys) and low detection limits (0.035 μM for Hg 2+ and 0.24 μM for Cys). The method was successfully applied to detect Hg 2+ and Cys in real samples, showcasing its potential for environmental monitoring and biomedical applications. This work not only provides a simple strategy for synthesizing highly active nanozymes but also highlights their potential in biosensing and catalysis.
研究领域:
生物化学
·
纳米生物学
·
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
Journal of Nano Research
·
2017
·
IF 0.511
Wen Juan Dong,Ji Yan Han,Xin Wu,Li Fan,Wen Ting Liang
A novel near-infrared fluorescence quenching method has been developed for the determination of hydrogen peroxide based on N-acetyl-L-cysteine-capped gold nanoparticles (NAC-AuNPs) as a fluorescence probe. The prepared gold nanoparticles with the size of about 1.91 nm exhibited strong near-infrared fluorescence emission at 693 nm with excitation wavelength at 450 nm in aqueous solution. The fluorescence intensity of NAC-AuNPs was quenched dramatically by adding hydrogen peroxide. Therefore, it could be used to detect hydrogen peroxide based on the fluorescence quenching intensity was linear with the concentration of hydrogen peroxide. Under the optimal experimental conditions, the linear range and detection limit were 1.0×10-6 –3.0×10-2 mol/L and 1.0×10-7 mol/L, respectively. The possible quenching mechanism was investigated by time-resolved fluorescence spectroscopy. The proposed method was simple, sensitive and showed good repeatability and stability.
研究领域:
分析化学
·
生物医学工程
·
纳米技术
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2013
·Vol. 52
·期 3
·1724-1729
·
IF 1.123
Xu Xiaobian,Miao Yelian,Chen Jie Yu,Zhang Qimei,Wang Jining
S-allyl-L-cysteine (SAC) is a bioactive compound in garlic ( Allium sativum ). A novel process including soaking and homogeneous reaction was applied for the effective production of SAC with endogenous γ-glutamyltranspeptidase (γ-GTP, EC 2.3.2.2) in garlic. The effects of temperature and CaCl 2 concentration on γ-GTP activity in soaking, and the relationship of SAC production with γ-GTP activity in homogeneous reaction were investigated, using fresh garlic as raw material. The experimental results showed that the γ-GTP in fresh garlic was activated by soaking. The yield rate and the final content of SAC increased linearly with increasing initial γ-GTP activity in the homogeneous reaction at 37 °C. The final SAC content reached 606.3 μg/g (i.e. 32 times higher than that in fresh garlic) after soaking for 72 h in a 10-mM CaCl 2 solution at 10 °C, and the homogeneous reaction for 8 h at 37 °C. SAC was produced effectively through the homogeneous reaction with activated endogenous γ-GTP in garlic.
研究领域:
植物学
·
生物化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ting Zhuo,Jun-ru Qi,Jin-song Liao,Hui-ying Wei,Rong Xiao
Konjac glucomannan (KGM) is a natural functional polysaccharide, and deacetylation (removing acetyl groups) impacts its physicochemical and gel properties. In order to study KGM's gel properties and explore new deacetylation modifiers, three deacetylated konjac glucomannan (DKGM) with different deacetylation degrees (DD, 17.51 %, 35.41 %, and 54.52 %) were prepared using L-arginine. Results showed that L-arginine deacetylated and retained the main structure and high molecular weight of KGM. Solubility decreased from 85.38 % to 81.17 %, transparency decreased from 38.52 % to 35.73 %, water absorbing and swelling slowed down, and molecular morphology changed from chain structures to micelle-like aggregates after deacetylation. Dynamic viscoelastic analysis showed that deacetylation facilitated the formation of the entangled network structure of KGM. The strength of konjac glucomannan and κ-carrageenan mixed gels increased after deacetylation, and this enhancement was mainly through hydrogen bonds and hydrophobic interactions. These findings will provide theoretical guidance for the application of KGM.
研究领域:
食品科学
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 6
·1277
·
IF 6.525
Huimin Wu,Yuchen Xu,Mengru Cai,Longtai You,Jing Liu,Xiaoxv Dong,Xingbin Yin,Jian Ni,Changhai Qu
The incidence of ocular surface disease (OSD) is increasing, with a trend towards younger ages. However, it is difficult for drugs to reach the deep layers of the cornea due to ocular surface barriers, and bioavailability is less than 5%. In this study, DSPE-PEG2000 was modified with L-valine (L-Val), and an HS15/DSPE-PEG2000-L-Val nanomicelle delivery system containing baicalin (BC) ([email protected]/DSPE-PEG2000-L-Val) was constructed using thin-film hydration, with a high encapsulation rate, small particle size and no irritation to the ocular surface. Retention experiments on the ocular surface of rabbits and an in vivo corneal permeation test showed that, compared with the control, nanomicelles not only prolonged retention time but also enhanced the ability to deliver drugs to the deep layers of the cornea. The results of a protein inhibition and protein expression assay showed that nanomicelles could increase uptake in human corneal epithelial cells (HCEC) through energy-dependent endocytosis mediated by clathrin, caveolin and the carrier pathway mediated by PepT1 by inhibiting the overexpression of claudin-1 and ZO-1 and suppressing the expression of PepT1-induced by drug stimulation. These results indicate that[email protected]/DSPE-PEG2000-L-Val is suitable for drug delivery to the deep layers of the ocular surface, providing a potential approach for the development of ocular drug delivery systems.Keywords:L-valine;nanomicelles;baicalin;PepT1;endocytosis proteins;tight junction proteins;ocular drug delivery system
研究领域:
药学
·
眼科学
·
纳米技术
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
E Yang,Meng Jun,Cai Heqing,Li Caibin,Liu Sainan,Sun Luming,Liu Yanxiang
The organic compounds from biochar play a role of hormone analogs, stimulating the expression of metabolites by controlling related gene and protein. In this experiment, we reported the L-histidine biosysthesis was promoted by biochar treatment in E. coli unlike genetic engineering of the traditional method. The related results indicated the most optimal concentration was found to be 3%, and 7% is the lethal dose. E. coli was inhibited in the high-concentration treatment. On the other hand, docking technology was usually used as drug screening, basing on Lock-and-key model of protein in order to better understand mechanisms. So the organic compounds of biochar from GC-MS analysis that acted as ligands were connected to HisG protein controlling L-histidine biosysthesis in E. coli. The result showed that the three organic molecules interacted with HisG protein by hydrogen bond. So we considered that these three compounds play regulatory roles in L-histidine biosysthesis, and the hisG gene expression fully supports this conclusion.
研究领域:
微生物学
·
生物化学
·
分子生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
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