SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yue Wang,Shuning Lei,Youjun Zeng,Wei Ni,Jie Zhou,Guo-Jun Zhang
MicroRNA(miRNA) has emerged as a particularly promising biomarker for the early diagnosis of Alzheimer's disease (AD). However, current detection methods for miRNA remain challenges in terms of sensitivity and pre-amplification steps. This study proposes a novel surface plasmon resonance imaging (SPRi) biosensor that combines tetrahedral DNA nanostructures (TDN)-based surface organization with CRISPR-Cas13a-mediated molecular recognition, enabling amplification-free, sensitive, and specific detection of AD-related miRNA-137. The biotin-labeled RNA reporters are anchored to TDN framework on the gold film surface, ensuring the orientation of the probe. In the presence of target miRNA-137, the CRISPR-Cas13a system activates, cleaves these reporters and prevents subsequent binding of streptavidin-HRP, thereby inhibiting precipitate formation from the enzymatic reaction. Under optimal condition, this sensor, integrated with the self-developed highly sensitive intensity-modulated SPRi system, displays a good linear response to miRNA-137 ranging from 0.5 pM to 500 nM with a detection limit of 212 fM. The platform demonstrates excellent specificity of discriminating single-base mutation, and reliable analysis capability in clinical plasma samples. Notably, our sensor confirms significantly reduced miRNA-137 levels in AD patients compared to healthy people. This innovative assay provides a powerful tool for the detection of AD-related miRNAs, promising to advance early diagnosis research for AD.
研究领域:
分子诊断
·
神经生物学
·
生物传感技术
·
纳米医学
·
转化医学
·
分子生物学
·
临床检验
✓ 已验证
产品关联
DOI
高影响因子
Journal of Analysis and Testing
·
2025
·
IF 7
Ren Dandan,Dong Wenpei,Shen Shiqi,Zhao Wenchen,Qian Tianwei,Xia Xinyi,Li Yuqiu,Xu Fuxin,Tang Xiaoya,Li Xingchen,Chen Yu,Zhang Jue,Cheng Xia,Yang Jianjun,Yu Ping
Colorectal cancer (CRC) is a gastrointestinal malignancy with high morbidity and mortality rates. Timely and effective early diagnosis is crucial for improving the survival rate of CRC patients. However, current histopathological biopsy techniques are not only invasive but also fail to fully capture the biological characteristics and dynamic changes of CRC, making them ineffective for early identification. Since dysregulated microRNA (miRNA) signatures are associated with various diseases, including cancers, miRNA analysis in liquid biopsies offers a promising approach for cancer diagnosis, prognosis and monitoring. Nonetheless, single biomarker profiling is no longer sufficient to meet the specificity required for early CRC screening. It is expected that multiple miRNA analyses will enable both early CRC identification and dynamic monitoring. In this work, we synthesized a fluorescent metal-organic framework (UiO-66-NH 2 ) with desirable optical properties and successfully developed a ratiometric fluorescence sensor. This method enabled the highly sensitive and precise simultaneous quantification of CRC-related miRNA-21, miRNA-17 and miRNA-92a. It has also shown excellent performance in clinical samples. The integrated detection of multiple CRC-related miRNAs enhanced data accuracy through unique probe designs, offering significant potential for widespread applications.
研究领域:
肿瘤学
·
生物传感器
·
纳米材料
·
分子诊断
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 19
·11257-11262
·
IF 3.119
Xitong Huang,Zhiming Xu,Ji-Hua Liu,Bo-Yang Yu,Jiangwei Tian
MicroRNA-21 (miRNA-21) is a significant biomarker which is closely related to some kinds of diseases, such as cancer, cardiovascular disease and kidney disease. Therefore, the detection of miRNA-21 is of great importance and can provide essential information for disease diagnosis. In this study, we report a facile, sensitive assay for miRNA-21 detection using personal glucose meters (PGM). Biotinylated DNA strand linked invertase (Inv) is conjugated on the surface of streptavidin-coated magnetic beads (MBs) to form a MBs–DNA–Inv complex. Target miRNA-21 in the detection system is captured by the MBs–DNA–Inv probe through DNA/RNA hybridization. The duplex-specific nuclease (DSN) enzyme specifically cleaves the DNA to recycle the target miRNA and release invertase, thereby triggering the dual signal amplification and ensuring high sensitivity. Besides, we establish a linear relationship between PGM and different concentrations of miRNA-21 in the range of 10 to 200 pM. The limit of detection is 1.8 pM, which is more sensitive than some of the previous reports. In addition, the biosensor exhibits excellent sequence selectivity and single-base mutation can be discriminated. Moreover, the expression of miRNA-21 is confirmed in urine from mice by our method, which is in good accordance with the qRT-PCR result. Therefore, a dependable, low-cost strategy for the detection of miRNA has been established and it meets the latest analytical demands for miRNA determination that is suitable for the public.
研究领域:
生物传感器
·
分子生物学
·
分析化学
·
疾病诊断
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2014
·Vol. 18
·期 10
·2829-2835
·
IF 2.234
Wang Mo,Yin Huanshun,Fu Zhengliang,Guo Yunlong,Wang Xinxu,Zhou Yunlei,Ai Shiyun
MicroRNAs (miRNAs), a class of small endogenous nonprotein-coding RNAs, regulate a wide range of biological processes, and their abnormal expressions are related to the growth and development of plants. Thus, a simple, rapid, and highly sensitive assay for miRNA detection is of great significance. In this work, a label-free and ultrasensitive assay for miRNA detection using protein cage nanoparticles has been developed. Apoferritin-encapsulated Cu nanoparticles (Cu-apoferritin) could be immobilized on the electrode through special reaction between amino and carboxyl. Hybridization event between the probe DNA and the target miRNA-159a is confirmed by electrochemical oxidation signal after Cu released into the detection buffer by adjusting the pH. This assay is highly selective and sensitive with a low detection limit of 3.5 fM. Moreover, the developed method can even discriminate single-base mismatched strand between the complementary targets. The effect of abscisic acid on the expression level of miRNA-159a in Arabidopsis thaliana seeds was also investigated.
研究领域:
植物学
·
遗传学
·
纳米技术
·
电化学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2012
·Vol. 2
·期 18
·7140-7145
·
IF 0
Yunlei Zhou,Mo Wang,Xiaomeng Meng,Huanshun Yin,Shiyun Ai
A novel signal amplified electrochemical microRNA (miRNA) biosensor was developed here using hemin-G-quadruplex as signal unit. A hairpin structure DNA probe (43 bases) was immobilized on the gold nanoparticles (AuNPs) modified gold electrode surface with the segment at its 3′-end complementary to miRNA-21 (22 bases) and the segment at its 5′-end complementary to capture DNA (21 bases). Upon hybridization with the target miRNA-21, the hairpin structure was unfolded, which was further hybridized with capture DNA loaded on AuNPs. The AuNP contained two kinds of DNA; one was complementary to the hairpin structure DNA probe, while the other was aptamer for hemin. The electrochemical signal of hemin self-assembled in the center of G-quadruplex structure of the aptamer was measured using chronoamperometry. The miRNA-21 target was analyzed with a detection limit of 3.96 pM. Using isolated total RNA from human gastric carcinoma BGC-823 cells, human breast adenocarcinoma MCF-7 cells, human hepatocarcinoma HepG2 cells and human colon cancer HT-29 cells, the assay detected specifically miRNA-21 and over-expression of oncogene miRNA-21 was demonstrated.
研究领域:
生物化学
·
生物技术
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Jia Tang,Yuxin Xing,Zhenqiang Wang,Mengnan Yang,Jixi Zhang,Kaiyong Cai
The design and interfacial engineering of DNA functionalized nanosensors with spatially separated but functionally integrated detection units holds a great promise in optical biosensors. In this study, we developed a bi-component hybrid of Janus nanoquencher particles with immobilized enzymes for fluorescence-based “off-on” detection in solution. Specifically, the hybrids consisting of a mesoporous polydopamine nanoparticle (MPDA) side (∼200 nm) asymmetrically attached with a gold (Au) particle side (∼30 nm) were generated through electrostatic repulsion-controlled growth. By regioselective binding and modification of the exposed side of the particles, fluorescent DNA probes and duplex-specific nuclease (DSN)/T7 exonuclease (T7) were subcompartmentalized on the MPDA and Au side, respectively, to realize substrate recognition and target recycling via the formation and hydrolysis of DNA hybrids. By using microRNAs (miRNAs) as a model target, the developed nanosensor realized a sensitive detection with a low limit of 32 fM (in a linear range from 20 fM–500 fM). Additionally, a high selectivity capable of discriminating a single-base mismatch, as well as a long-term detection stability were realized by the asymmetric interface designs and enzyme immobilization. The methodology is promising in the design and development of DNA probe-loaded hybrid nanosensors.
研究领域:
生物传感器
·
纳米技术
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-8
·
IF 5.479
Chen Donghua,Wen Siming,Peng Rulin,Gong Qingsong,Fei Junjie,Fu Zhuo,Weng Chao,Liu Minna
Mesoporous silica nanospheres (MSNs) are used in a triple signal amplification chemiluminescent (CL) assay for microRNA-21. It is based on (a) the synergistic amplification via loading and controlled-release of signal reagents by MSNs, (b) target recycling amplification, and (c) the enhancement effect of graphene oxide quantum dots (GOQD). CL is generated by the bis(2,4,6-trichlorophenyl) oxalate (TCPO) and H 2 O 2 reaction in the presence of the fluorophore rhodamine B (RB). RB is firstly loaded into the pores of MSNs modified with amino groupsand coupled with ssDNA. Then, the pores are capped by GOQD. Upon the addition of microRNA-21 into the system, the designed ssDNA assumes a double stranded structure. With the aid of duplex-specific nuclease, the double strand structure is cleaved and the free microRNA-21 enters into the next cycling process to combine with other ssDNA forming double strand structures. After several cycling process, amounts of GOQDs departing from the surface of MSNs cause the opening of the pores of MSNs and the release of RB causes the CL of TCPO-H 2 O 2 reaction system. Free GOQDs can lead to a further CL enhancement. By this method, even a low amount of microRNA-21 leads to a large number of released RB molecules and triggers high-intensity CL. The method was applied in an assay where the CL signal increases linearly with the logarithm of the microRNA-21 concentration in the range of 0.005–50 pmol L −1 and the detection limit is 1.7 fmol L −1 (at 3σ).
Schematic presentation of a triple signal amplification chemiluminescence (CL) analysis platform based on rodamine B (RB) loading and controlled release, target recycling amplification and graphene oxide quantum dots (GOQD) as the enhancer for analysis of microRNA-21 in human serum.
研究领域:
癌症研究
·
分析化学
·
纳米技术
·
生物标志物检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Lina Wu,Yingfei Wang,Rong He,Yue Zhang,Yuling He,Chao Wang,Zhenda Lu,Ying Liu,Huangxian Ju
Despite the great success of fluorescence sensing array application in bioanalysis , the photo-quenching problem accompanied with high surface fluorophore density has become the bottleneck for its further sensitivity improvement. Herein, a fluorescence polyethylene glycol (PEG) hydrogel array was developed for highly sensitive microRNA detection based on interfacial cation exchange amplification coupled with DNA hybridization chain reaction (HCR). The carboxylate PEG hydrogel array was fabricated on glass slide via photo polymerization and modified with miRNA capture probe, target miRNA binding triggered HCR in hydrogel with biotin labelled DNA probes to generate numerous DNA polymer chains where biotinylated CdS QDs were subsequently conjugated with a streptavidin bridge. Interfacial cation exchange amplification was triggered in hydrogel upon the introduction of Ag + and Rhod-5N, and abundant Cd 2+ was released from CdS to bind with Rhod-5N for substantial fluorescence enhancement. The aqueous-like environment of hydrogel eliminated the fluorescence quenching and simplified experiment process by performing cation exchange reaction at interface with direct result. The linear range for model target miRNA-21 was 1 fM to 500 pM with a detection limit of 0.835 fM. Taking advantage of the nonfouling property of PEG hydrogel, direct quantification of miRNA-21 was achieved from crude cancer cell lysates with a detection limit down to 10 equivalent cells. The expressions of circulating miRNA-21 in clinical serum samples were also assessed with comparable results from RT-PCR. The developed hydrogel array provides a universal platform for highly sensitive fluorescence sensing, and would benefit clinical non-invasive disease diagnosis.
研究领域:
生物化学
·
生物技术
·
分析化学
·
分子生物学
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Zilian Jiang,Jiao Jiao,Jianjun Li,Hongfang Zhang,Jianbin Zheng
MicroRNAs are novel and reliable biomarkers for early cancer diagnosing. However, accurate detection of the ultralow amount of a specific microRNA remains a big challenge. Herein, an electrochemical biosensing platform based on bivalent recognition-induced nanoparticle blocking in nanochannels for signal amplification is developed. Bivalent recognition is realized by two oligonucleotides, individually containing the complementary sequence for opposite ends of the target microRNA. One oligonucleotide is anchored into the alumina nanochannels and the other is bonded onto the SiO 2 nanoparticles. The presence of the target microRNA increases the blockage effect of the nanochannels and which is greatly amplified by bringing the nanoparticles into the nanochannels. Methylene blue (MB) is chosen as the reporter of the blockage effect. The amount of MB fluxing through the nanochannels is monitored at a working electrode functionalized with MoS 2 nanoplates and treated by the electrochemical method to further amplifying the signal. The reductive current of MB is quantitatively dependent on the concentration of microRNA. Using microRNA-21 as the model target, the electrochemical signal exhibited a linear dependence on the concentration of the target in a wide range of 100 aM to 1 nM. The platform could be readily extended to other nucleic acids.
研究领域:
生物技术
·
医学诊断
·
纳米科技
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jiasheng Wang,Ni Hui
A simple electrochemical patterning strategy has been developed for the construction of ultrasensitive biosensors based on polyethylene glycol (PEG)-polypyrrole (PPy) nanowires substrate in order to supply improved antifouling performances. PPy nanowires array was produced through electrochemical polymerization of pyrrole at constant potential onto glassy carbon electrode (GCE) surface. PPy nanowires surfaces were decorated with 4-armed PEG molecules by electrochemical oxidation of amine groups offered by PEG end chains. The prepared PEG/PPy nanowires have integrated the excellent electrical conductivity of conducting polymer PPy nanowires with the good antifouling property of PEG. MicroRNAs (miRNAs) play very important roles in cancer development and a variety of diseases, which make them become promising biomarkers of the onset, prognosis and risk of diseases. Therefore the establishment of miRNA profiles for diseases and the detection of miRNAs in biological samples are critical milestones in diagnostics. An ultrasensitive electrochemical biosensor for miRNA can be effortlessly developed by the immobilization of DNA probes onto PEG/PPy nanowires. DNA/RNA hybridization was monitored by changes of methylene blue (MB) redox signal using differential pulse voltammetry (DPV) method. A wide linear range (0.10 pM ∼ 1.0 nM) to target miRNA was obtained by the fabricated biosensor, and miRNA mismatches can also be easily identified with satisfactory. Furthermore, the biosensor based on PEG/PPy nanowires can be extended to the development of any type of DNA-based biosensor.
研究领域:
生物传感
·
分子诊断
·
电化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2016
·Vol. 11
·期 3
·e0149257
·
IF 3.057
Chao Fang,Weihong Lu,Chengyan Li,Xi Peng,Yang Wang,Xiulan Huang,Zhihong Yao,Nali Cai,Yuge Huang,Xingliang Zhang,Jianxin Tan
Asthma is a common chronic respiratory disease. In a previous study, we found several circulating microRNA signatures associated with childhood asthma and selected miR-3162-3p for subsequent studies. Since the target proteins and underlying molecular mechanisms of miR-3162-3p in asthma etiopathogenesis are not well characterized, we designed this study to clarify its role. We employed bioinformatics and quantitative PCR methods as a first step to determine the target of miR-3162-3p, and we elucidated β-catenin. Luciferase assays and western blot analysis confirmed β-catenin as a direct target of miR-3162-3p as the 3’-untranslated region of β-catenin mRNA possesses a specific miR-3162-3p pairing site. The correlation between the expression levels of miR-3162-3p and β-catenin is confirmed by quantitative PCR and western blot studies in A549, Beas-2B and H1299 cell lines and OVA-induced asthma mouse model. Of note, upregulation of the endogenous miR-3162-3p level is concomitant with the reduction of β-catenin mRNA and protein expression levels. MiR-3162-3p antagomir treatment antagonizes the endogenous miR-3162-3p and effectively rescues the attenuation of endogenous β-catenin in OVA-induced asthmatic mice, which alleviates airway hyperresponsiveness and ameliorates airway inflammation. Collectively, our findings suggest a novel relationship between miR-3162-3p and β-catenin and clarify their mechanistic role in asthma etiopathogenesis.
研究领域:
分子生物学
·
呼吸病学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jiaxin Su,Jinya Du,Rujiao Ge,Chenyang Sun,Yuchun Qiao,Wei Wei,Xuejiao Pang,Yufan Zhang,Huiting Lu,Haifeng Dong
DNAzyme shows great promise in designing a highly sensitive and specific sensing platform; however, the low cellular uptake efficiency, instability, and especially the insufficient cofactor supply inhibit the intracellular molecule sensor applications. Herein, we demonstrate a novel type of DNAzyme-based self-driven intracellular sensor for microRNA (miRNA) detection in living cells. The sensor consists of a metal–organic framework [zeolite imidazole framework (ZIF-8)] core loaded with a shell consisting of a rationally designed DNAzyme, where the substrate strand is modified with FAM and BHQ-1 nearby both the sides of the restriction site, respectively, while the enzyme strand consists of two separate strands with a complementary fragment to the substrate strand and the targeting miRNA, respectively. The ZIF-8 nanoparticles enable the efficient delivery of DNAzyme into the cell and protect the DNAzyme from degradation. The pH-responsive ZIF-8 degradation is accompanied with the release of the DNAzyme and Zn2+ cofactors, and the intracellular target miRNAs recognize and activate the DNAzyme driven by the Zn2+ cofactors to cleave the substrate strand, resulting in the release of the FAM-labeled shorter product strand and increased fluorescence for miRNA detection. The self-driven approach can be generally applied to various miRNAs’ detection through DNAzyme engineering.
研究领域:
生物化学
·
分子生物学
·
纳米技术
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）