Food Chemistry-X
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2024
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IF 6.5
Mengya Zhao,Hong Ren,Zhuomin Yan,Jing Ma,Xiaoping Feng,Di Liu,Fangyu Long
Ochratoxin A (OTA) contamination in grape juice has attracted widespread concern as OTA can lead to kidney disease and cause adverse neurological effects. An effective method to remove OTA is to make use of highly adsorbent materials that are able to remove the toxic contaminant. Recently, inactivated Lactobacillus plantarum- based biosorbents have shown to be an efficient, cost-effective and environmentally friendly bioremediation method in removing toxic pollutants such as OTA. We used five chemical thiol-modification methods to improve the adsorption efficiency of OTA in grape juice. The esterification of Lactobacillus plantarum (L-Es) significantly increased the sulfhydryl contents (-SH) by 251.33 μmol/g and >90% of OTA was removed. However, the inactivated microbial adsorbent was difficult to separate after adsorption and therefore, the prepared L-Es were embedded into the cellulose nanocrystals (L-Es@CNCs). Moreover, L-Es@CNCs significantly increased the adsorption rate of OTA in grape juice samples by 88.28% with negligible effects on juice quality due to the properties of easy re-use and excellent biodegradability. This showcases its potential application for OTA removal in the grape juice industry.
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SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Jing Zhang,Xiaojian Xu,Yue Qiang
An ultrasensitive electrochemical ochratoxin A (OTA) aptasensor was designed using AgPt bimetallic nanoparticles decorated iron-porphyrinic metal-organic framework (PCN-223-Fe) as electrochemical tracer. PCN-223-Fe was synthesized with zirconium ion as node and iron-porphyrin as linker, and in situ supported with PtAg bimetallic nanoparticles to obtain AgPt/PCN-223-Fe composite. Spindle-shaped AgPt/PCN-223-Fe was characterized by electron microscopies, X-ray diffraction and X-ray photoelectron spectroscopy. Due to the synergistic effect, AgPt/PCN-223-Fe modified electrode exhibited enhanced electrocatalytic activity to oxygen reduction. Sequentially, AgPt/PCN-223-Fe was combined with streptavidin (SA) to prepare electrochemical tracer. SA/AgPt/PCN-223-Fe introduced into the electrode surface capturing OTA aptamer through the recognition of biotin and streptavidin, which lead to a strong electrochemical signal from oxygen reduction. When OTA existed in solution, the aptamer was bonded with OTA preferentially, and electrochemical signal reduced. The proposed approach showed a wide linear range from 20 fg mL −1 to 2 ng mL −1 and a low detection limit of 14 fg mL −1 (S/N = 3). Meanwhile, the aptasensor showed good reproducibility, stability and selectivity, which was successfully applied for OTA detection in red wine and corn samples. This strategy broadens application of metal-organic frameworks, and could be used for other toxins detection.
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NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 27
·11977-11982
·
IF 3.591
Wenqiang Lai,Jiaqing Guo,Zuqin Qiao,Xuwei Chen,Shuhan Wang,Luxi Wu,Quanying Cai,Shuai Ye,Youxiu Lin,Dianping Tang
Biotoxins present in foods represent a major source of contamination. Although different analytical methods have been developed, a new detection method for identification and quantification of these toxins is necessary for extending their application sphere. Therefore, in this study, an enzyme-controlled citrate-iron(III) chelating system was used to build a novel colorimetric immunoassay sensing platform for detection of ochratoxin A (OTA). The experimental signal was derived from the reaction of iron(III) and 3,3′,5,5′-tetramethylbenzidine (TMB). However, the addition of citrate could combine with iron(III) rapidly, thereby hindering the reaction of iron(III) and TMB for production of the signal (i.e., colorless to blue). Additionally, hydrogen peroxide (H2O2), which could be produced from glucose in the presence of glucose oxidase (GOx), could affect the binding capacity of citrate and iron(III), thereby releasing iron(III) to react with TMB. That is to say, different amounts of GOx could regulate the intensity of the signal. Based on these findings, magnetic bead (MB)-based OTA competed with dissociative OTA (added) for glucose oxidase (GOx)-labelled anti-OTA. As the dissociative OTA concentration increased, the amount of GOx-labelled anti-OTA on MB conveniently reduced and the absorbance decreased. The method exhibited a good linear relationship as the concentration of the target (OTA) increased from 0.005 to 5 ng mL−1; the detection limit was 4.2 pg mL−1, as estimated based on the 3Sblank level under optimal conditions. The specificity and feasibility for a real sample of the colorimetric immunoassay was acceptable, respectively. The current results provided important insights into the development of citrate-iron(III) chelating system-based biological detection methods and colorimetric immunoassays.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Tao Liao,Jiaqiang Huang,Xukang Sun,María Belén Camarada,Xuemei He,Pin Zhang,Xiaoning Liao
The luminol–H 2 O electrochemiluminescence (ECL) system has received increasing attention in recent years owing to its ability to overcome the intrinsic drawbacks of conventional co-reactants, specifically the self-decomposition of hydrogen peroxide and low solubility of dissolved oxygen. However, this system still suffers from a high triggering potential, which leads to excessive oxygen (O 2 ) bubble accumulation at the electrode surface and unstable ECL signals. Herein, we proposed a new ECL enhancement strategy for the luminol–H 2 O system using multiwalled carbon nanotube (MWCNTs)-functionalized bimetallic cobalt–zinc zeolitic imidazolate framework (CoZn-ZIF) as a co-reactant accelerator. Benefiting from the synergistic interaction, the MWCNTs-modified CoZn-ZIF (CoZn-ZIF@MWCNTs) demonstrated superior oxygen evolution reaction activity at a low potential (0.6 V vs. Ag/AgCl), leading to stable and high-intensity ECL signals through efficient catalytic conversion of H 2 O molecules into hydroxyl radicals (·OH) and superoxide radicals (O 2 ·− ). By integrating molecular imprinting technology, a novel ECL sensor was developed for the detection of ochratoxin A, achieving a limit of detection of 0.023 nM (S/N = 3) and wide linear range (0.1–10,000 nM). In real sample analysis, the sensor showed satisfactory recoveries of 99.30 %–110.30 %, demonstrating high accuracy. This work not only establishes a new pathway for enhancing luminol ECL performance but also provides innovative insights for advancing ECL-based sensing technologies in food safety and environmental monitoring.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Caifeng Zou,Shi Feng,Zongyi Li,Meng Wu,Panhua Li,Yijing Wang,Yanyan Shang,Xiangyang Li
Ochratoxin A (OTA) and zearalenone (ZEN), two ubiquitous mycotoxins accumulating in warm, humid climates, contaminate crops and foods, making accurate on-site detection essential for safeguarding food safety and environmental health. In this study, carbon/niobium carbide nanomaterials (C/NbCNMs) with superior peroxidase-like activity were synthesized through a carbothermal reduction process followed by dilute-acid etching—a treatment that generated abundant active sites and a large specific surface area. Leveraging the nanozyme activity of C/NbCNMs and the specific recognition capability of aptamers, a dual-aptamer colorimetric sensing platform (C/NbCNMs@OTA-Apt and C/NbCNMs@ZEN-Apt) was constructed for the parallel detection of OTA and ZEN. Aptamer conjugation markedly enhanced the catalytic activity of the nanozyme, amplified the chromogenic signal, and enabled detection limits as low as 19 pg mL −1 (0.019 ng mL −1 ) for OTA and 16 pg mL −1 for ZEN, with visual detection limits of 60 and 50 pg mL −1 , respectively. For high-throughput analysis, a dual-channel intelligent model was established to predict toxin concentrations within 10 s via chromatic characteristic analysis. Moreover, the sensing platform exhibited excellent practicality and accuracy in naturally contaminated food samples, with relative standard deviations (RSD) of 3.30 % for OTA and 3.84 % for ZEN. This work offers a low-cost, highly sensitive, portable, and intelligent alternative to conventional mycotoxin detection methods, facilitating rapid on-site screening of OTA, ZEN, and other analogous contaminants.
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ANALYTICAL SCIENCES
·
2025
·
IF 2
Xia Shaojie,Yang Zhenyuan,Sun Xiaohui,Huang Junjie,Peng Kuan,Wang Yonghong
Ochratoxin A (OTA), a highly toxic mycotoxin, extensively contaminates a wide range of food commodities. Given increasing demands for sensitive and user-friendly detection platforms, we developed a colorimetric OTA biosensor leveraging copper-silver nanoclusters (Cu/Ag NCs), whose peroxidase-like activity is augmented via G/C-rich DNA hybridization. The system employs two tailored DNA strands: a template strand featuring a cytosine-rich domain for NC synthesis flanked by terminal recognition segments and an enhancer strand containing a guanine-rich signal amplification domain and a complementary linker. Upon OTA binding with the aptamer, the apt-template complex dissociates, facilitating template-enhancer hybridization to form G/C-rich double-stranded DNA. This structural transition substantially enhances the peroxidase-like activity of DNA-Cu/Ag NCs, accelerating the oxidation of 3,3′,5,5′-tetramethylbenzidine by H 2 O 2 while simultaneously amplifying the colorimetric signal. Quantitative OTA detection is accomplished by monitoring this dynamic color response. The biosensor demonstrates a semi-log linear detection range from 5.0 nM to 1.0 μM with a detection limit of 5.0 nM. Furthermore, the specific test has high selectivity for other common mycotoxins, and its applicability has been validated through real sample analysis, suggesting that the DNA-Cu/Ag NCs biosensor used for OTA detection holds promising practical applications in food samples. Graphical abstract
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INTERNATIONAL OPHTHALMOLOGY
·
2024
·Vol. 44
·期 1
·1-10
·
IF 1.4
Fu Miao,Chen Yuanyuan,Yang Anhuai
Purpose Ochratoxin A (OTA) contamination of food and feed is a serious problem worldwide. OTA is considered a carcinogen and immunotoxic, nephrotoxic, and neurotoxic mycotoxin. The present study aims to determine the toxic effects of OTA on retinal ganglion cells (RGCs) and assess the resulting impairment of retinal function in mice. Methods RGC-5 cells were exposed to OTA (100 and 200 μg/L) for 3 days, and the mice were fed OTA-contain (100 and 200 μg/kg) diets for 4 weeks. Antioxidant indices were detected by spectrophotometer. The apoptosis of RGC-5 cells was determined by flow cytometry. Mitochondrial morphology and mitochondrial membrane potential were detected by immunofluorescence. RGC survival was determined by immunofluorescence staining with Brn3a. Flash electroretinography (ERG) was conducted to assess visual function. Results The oxidative-antioxidant balance suggested that OTA-induced severe oxidative stress, including increased reactive oxygen species (ROS) and malondialdehyde (MDA) levels in the OTA-exposed RGC-5 cells, and the reduced activity of superoxide dismutase (SOD) and glutathione-S-transferase (GST) in the OTA exposed group. Furthermore, OTA exposure led to remarkable apoptosis in RGC-5 cells. The mitochondrial detection showed that OTA caused significant mitochondrial membrane potential reduction and mitochondrial fragmentation, which may be the cause of apoptosis of RGC-5 cells. Additionally, in vivo experiments demonstrated that OTA resulted in significant death of RGCs and subsequent retinal dysfunction in mice. Conclusion Ochratoxin A induces mitochondrial dysfunction, oxidative stress, and RGCs death in mice.
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FOOD CHEMISTRY
·
2026
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IF 9.8
Saiqun Wang,Keying Chu,Rong Guo,Yue Liu,Lu Cui,Zhouli Wang,Xubo Zhao
Ochratoxin A (OTA) is a widespread mycotoxin contaminating fruits and related products worldwide. Given its significant threats to public health and substantial economic consequences, the development of an efficient and safe detoxification strategy has become pressing necessity. This study synthesized Fe 3 O 4 @TiO 2 @PDA composites via sol-gel method and alkaline PDA grafting, capable of efficiently removing OTA in wines. The characterization results confirmed that Fe 3 O 4 @TiO 2 @PDA has a hierarchical structure consisting of Fe 3 O 4 cores, TiO 2 layers, and PDA coatings, with ~13.47 emu/g saturation magnetization. Fe 3 O 4 @TiO 2 @PDA showed enhanced methylene blue photocatalytic activity and acid tolerance compared with TiO 2 , and achieved 88.51% ochratoxin A (OTA) removal in wine via adsorption (82.83% in 2 h) and photocatalysis (5.68% in 2 h). After 8 cycles, the OTA removal efficiency remained at 33.10%. Degradation products were low-toxicity OTα and phenylalanine. These results validate Fe 3 O 4 @TiO 2 @PDA as a highly efficient photocatalyst for detoxifying OTA in wine.
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Foods
·
2022
·Vol. 11
·期 11
·1598
·
IF 5.561
Yinyin Liu,Dan Liu,Shuangshuang Cui,Can Li,Ziguang Yun,Jian Zhang,Fengxia Sun
In order to improve the weak optical performance of gold nanoparticles and realize the signal amplification of lateral flow chromatography test strips, individual gold nanoparticles (AuNPs) were aggregated into gold nanoparticle aggregates through functional groups around polyamidoamine (PAMAM) dendrimers. A signal-amplified aptamer-based lateral flow chromatography test strip was constructed for the rapid determination of ochratoxin A (OTA). Under optimal conditions, the visual detection limit of this test strip was 0.4 ng mL−1and the semi-quantitative limit of detection (LOD) was 0.04 ng mL−1. Compared with other traditional aptamer lateral flow chromatography test strips, its sensitivity was improved about five times. The whole test could be completed within 15 min. The aptamer-based strip was applied to the detection of OTA in red wine; the average recoveries ranged from 93% to 105.8% with the relative standard deviation (RSD) varying from 3% to 8%, indicating that the test strip may be a potentially effective tool for the on-site detection of OTA.Keywords:ochratoxin A;signal amplification;lateral flow test strip;aptamer;red wine
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Biosensors-Basel
·
2021
·Vol. 11
·期 3
·87
·
IF 5.519
Yasmin Alhamoud,Yingying Li,Haibo Zhou,Ragwa Al-Wazer,Yiying Gong,Shuai Zhi,Danting Yang
Ochratoxin A (OTA) primarily obtained by the generaaspergillusandpenicillium, is one of the toxic substances for different organs and systems of the human body such as the kidney, liver, neurons and the immune system. Moreover, it is considered to cause tumors and fetal malformation even at a very low concentration. Fast and sensitive assay for detection of OTA at ultralow levels in foods and agricultural products has been an increasing demand. In this study, a new label-free electrochemical biosensor based on three-dimensional reduced graphene oxide/gold nanoparticles/aptamer for OTA detection was constructed. The 3D-rGO/Au NPs nanocomposites were firstly synthesized using a one-pot hydrothermal process under optimized experimental conditions. The 3D-rGO/Au NPs with considerable particular surface area and outstanding electrical conductivity was then coated on a glass carbon electrode to provide tremendous binding sites for -SH modified aptamer via the distinctive Au–S linkage. The presence of OTA was specifically captured by aptamer and resulted in electrochemical impedance spectroscopy (EIS) signal response accordingly. The constructed impedimetric aptasensor obtained a broad linear response from 1 pg/mL to 10 ng/mL with an LOD of 0.34 pg/mL toward OTA detection, highlighting the excellent sensitivity. Satisfactory reproducibility was also achieved with the relative standard deviation (RSD) of 1.393%. Moreover, the proposed aptasensor obtained a good recovery of OTA detection in red wine samples within the range of 93.14 to 112.75% along with a low LOD of 0.023 ng/mL, indicating its applicability for OTA detection in real samples along with economical, specific, susceptible, fast, easy, and transportable merits.Keywords:Ochratoxin A;electrochemical biosensor;gold nanoparticles;three-dimensional reduced graphene oxide;aptamer
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ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yeru Wang,Yu Wang,Su Liu,Wenyu Sun,Manru Zhang,Long Jiang,Minghan Li,Jinghua Yu,Jiadong Huang
DNA walkers, as intelligent artificial DNA nanomachines, have been widely used as efficient nucleic acid amplification tools that the detection sensitivity can be improved by incorporating DNA walkers into DNA biosensors . Nevertheless, since the premature release or flameout in a region of locally exhausted substrate, the walking efficiency of DNA walkers remains unsatisfactory. In this work, we design a smart tripedal DNA walker that is formed by target-initiated catalyzed hairpin assembly (CHA), which can move along the DNA duplex tracks on electrode driven by toehold-mediated DNA strand displacement (TMSD) for transduction and amplification of electrochemical signals. Emphatically, this flexible tripedal DNA walker is capable of walking freely along the tracks with unconstrained walking range. Moreover, the design of multi-legged walker can weaken the derailment of leg DNA and shorten the moving time on electrode, ensuring the processive walking with high efficiency. Additionally, the persistent walking of tripedal walker is driven by cascading TMSD, which eliminates the defects of high cost and instability of enzyme-assisted amplification technology . Therefore, the tripedal DNA walker-based electrochemical biosensor has enormous potential for the applications of OTA detection, and reveals a new avenue for food safety analysis and clinical diagnosis.
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ACS Sensors
·
2020
·
IF 7.333
Xiao-Bin Huang,Shao-Hua Wu,Hao-Cheng Hu,Jian-Jun Sun
The “turn-on” mode surface-enhanced Raman scattering (SERS) aptasensor for ultrasensitive ochratoxin A (OTA) detection was developed based on the SERS “hot spots” of AuNanostar@4-MBA@Au core–shell nanostructures (AuNS@4-MBA@Au) and exonuclease III (Exo III)-assisted target cycle amplification strategy. Compared with conventional gold nanoparticles, AuNS@4-MBA@Au provides a much higher SERS enhancement factor because AuNS exhibits a larger surface roughness and the lightning rod effect, as well as an excellent electromagnetic field between the AuNS core and the Au shell, which contribute to the superstrong SERS signal. Meanwhile, Exo III-assisted target cycle amplification can be used as an effective method for the further amplified detection of OTA. Additionally, the utilization of streptavidin magnesphere paramagnetic particles offers a green, economical, and facile technology for the accumulation and separation of the signal probe AuNS@4-MBA@Au from solution. All these factors lead to a significant enhancement of detectable signals and superhigh sensitivity. As a result, the limit of detection as low as 0.25 fg mL–1 could be achieved, which was lower than that in the other reported literatures on SERS methods for OTA detection as we know. The developed SERS aptasensor also provides a promising tool for foodstuff detection.
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