Scientific Reports
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2024
·Vol. 14
·期 1
·1-10
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IF 3.8
Li Jiawei,Cheng Yi,Huang Yingchun,Xu Lixin,Zhang Kun,Yan Fei,Chen Jian,Lang Chunhui
Alkaline phosphatase (ALP) is a reliable biomarker for various diseases, making the development of new detection methods highly significant. Herein, we report a simple and accurate dual-signal optical detection strategy for measuring ALP activity across the entire clinical range in adults. Based on this, we designed a smartphone app to eliminate the limitations of large instruments, enabling real-time field detection. In this work, ALP catalyzes the conversion of the phosphate monoester of 2-phospho-L-ascorbic acid (PAA) into ascorbic acid (AA), a compound with strong reducing properties. AA facilitates the in-situ reduction of Ag + to form Au@Ag core-shell nanoparticles, accompanied by significant changes in the ultraviolet-visible absorption spectra and color. These changes allow for the qualitative and quantitative detection of ALP activity. In UV absorption spectroscopy and colorimetric detection, the signal shows good linearity with ALP activity concentrations, with detection ranges of 0 U/L to 200 U/L (R 2 =0.9961) and 25 U/L to 250 U/L (R 2 =0.9771), respectively. Subsequent selective analysis and serum sample analysis demonstrated the high selectivity and accuracy of the proposed method, highlighting its potential for field application.
研究领域:
生物标志物检测
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纳米技术
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医学诊断
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移动健康应用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·
IF 2.7
Huiying Li,Shuichang Chen,Hongjian Huang,Yan Fang,Yunxiang Weng,Qinhui Chen,Xinqing Liu,Haiqing Liu
In circumstances of severe traumatic bleeding, urgent bleeding control is vital to the wellness and life of the wounded person. Among many hemostatic measures, gauze is widely used because of cost-effectiveness, safety and efficiency. However, it may suck a large amount of blood before bleeding stops. Thus, advanced hemostatic gauzes less blood loss and quick hemostatic rate and is greatly demanded. Herein, a novel tissue adhesive gauze (TA-gauze) is developed by coating a thin layer of polyethyleneimine/poly(acrylic acid) (PEI/PAA) on the plain cotton gauze. This TA-gauze has a reduced hydrophilicity and gets a new trait of wet tissue adhesiveness from the PEI/PAA coating layer. The TA-gauze shows much improved hemostatic performance in the rat injury models, that is, its blood loss in the rat femoral artery model is just 13% that of the cotton gauze, due to it can retard the blood movement in the gauze and at the seam of gauze/tissue through a synergistic effect of tissue adhesion and hygroscopicity. It is biocompatible and on-demand detachable. The simple preparation procedure and high hemostatic efficiency of the TA-gauze may promise a bright future for an ideal hemostatic dressing for bleeding control.
研究领域:
生物材料
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止血
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组织工程
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Energy Chemistry
·
2023
·
IF 13.1
Xuhao Liu,Runzhe Yao,Siqi Wang,Yaqing Wei,Bin Chen,Wei Liang,Caiyun Tian,Chengyu Nie,De Li,Yong Chen
The emerging SiP 2 with large capacity and suitable plateau is proposed to be the alternative anode for Li-ion batteries. However, typical SiP 2 still suffers from serious volume expansion and structural destruction, resulting in much Li-consumption and capacity fading. Herein, a novel stretchable and conductive Li-PAA@PEDOT:PSS binder is rationally designed to improve the cyclability and reversibility of SiP 2 . Interestingly, such Li-PAA@PEDOT:PSS hydrogel enables a better accommodation of volume expansion than PVDF binder (e.g. 5.94% vs. 68.73% of expansivity). More specially, the SiP 2 electrode with Li-PAA@PEDOT:PSS binder is surprisingly found to enable unexpected structural recombination and self-healing Li-storage processes, endowing itself with a high initial Coulombic efficiency (ICE) up to 93.8%, much higher than PVDF binder (ICE = 70.7%) as well. Such unusual phenomena are investigated in detail for Li-PAA@PEDOT:PSS, and the possible mechanism shows that its Li-PAA component enables to prevent the pulverization of SiP 2 nanoparticles while the PEDOT:PSS greatly bridges fast electronic connection for the whole electrode. Consequently, after being further composited with carbon matrix, the SiP 2 /C with Li-PAA@PEDOT:PSS hydrogel exhibits high reversibility (ICE> 93%), superior cyclability (>450 cycles), and rate capability (1520 mAh/g at 2000 mA/g) for LIBs. This highly stretchable and conductive binder design can be easily extended to other alloying materials toward advanced energy storage.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
DOI
高影响因子
International Journal of Nanomedicine
·
2025
·
IF 6.5
Yilu Zheng,Gareth R Williams,Ran Hu,Sen Tong,Jianxiang Xu,Tong Wang,Yanyan Zhang,Junzi Wu,Fan Li,Yingyu Cai,Li-Min Zhu
Background: The development of selective formulations able to target and kill tumor cells without the application of external energy has shown great promise for anti-tumor therapy.Methods: Here, we report a “nanobomb” that explosively increases Ca content within cells. It can selectively release Ca2+ and generate H2O2 in the tumor microenvironment (TME) by acid-triggered degradation of the two-layer protective shell (ie, unlocking the “double-lock”). This material, termed CaO2@ZIF8:CUR@PAA, comprises a CaO2 core coated with the ZIF-8 framework, which was then loaded with curcumin (CUR) and coated again with polyacrylic acid (PAA).Results: Under the slightly acidic conditions of the TME, the PAA shell (first lock) breaks down first exposing CaO2@ZIF8 and CUR inside the cell. Then, ZIF8 (second lock) is degraded in response to acid to deposit Ca2+, and H2O2. CUR can promote the release of Ca2+ from the endoplasmic reticulum to the cytoplasm, inhibit the outflow of Ca2+, and accumulates a large amount of Ca2+ intracellularly together with exogenous Ca2+ (calcium storms). The powerful calcium storm that causes mitochondrial dysfunction. The presence of a large amount of exogenous H2O2 causes further oxidative damage to tumor cell membranes and mitochondria where intracellular ROS production far exceeds clearance. CaO2@ZIF8:CUR@PAA NPs can induce cell S cycle arrest and apoptosis to inhibit tumor multiplication and growth. Oxidative damage-triggered immunogenic cell death (ICD) in turn leads to the polarization of macrophages to the M1 phenotype, inducing immunogenic cell death and inhibiting tumor cell proliferation and metastasis.Discussion: The acid two-step unlocking nanoplatform is a therapeutic modality that combines calcium storm and oxidative damage. The mode triggers apoptosis leading to ICD of tumor cells. The material induces cycle blockade during treatment to inhibit cell proliferation. Robust in vitro and in vivo data demonstrate the efficacy of this approach and CaO2@ZIF8:CUR@PAA as an anticancer platform, paving the way for nanomaterials in immune-triggered cancer therapy.Highlights: - A new calcium accumulation nanoplatform plays a role in activate antitumor immunity.- Double-locked structure slows down the premature decomposition of CaO2.- The platform allows Ca2+ and H2O2 to accumulate in tumor cells.- This leads to mitochondrial dysfunction, apoptosis, and M1 macrophage polarization.- Potent anti-tumor effects are seen both in vitro and in vivo.
研究领域:
癌症治疗
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纳米技术
·
免疫学
·
细胞生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2019
·
IF 1.911
Yazhi Yang,Qianqian Lan,Yong Wang
Thin film composite (TFC) membranes represent a highly promising platform for efficient nanofiltration (NF) processes. However, the improvement in permeance is impeded by the substrates with low permeances. Herein, highly permeable gradient phenolic membranes with tight selectivity are used as substrates to prepare TFC membranes with high permeances by the layer-by-layer assembly method. The negatively charged phenolic substrates are alternately assembled with polycation polyethylenimine (PEI) and polyanion poly(acrylic acid) (PAA) as a result of electrostatic interactions, forming thin and compact PEI/PAA layers tightly attached to the substrate surface. Benefiting from the high permeances and tight surface pores of the gradient nanoporous structures of the substrates, the produced PEI/PAA membranes exhibit a permeance up to 506 L ∙ m −2 ∙ h −1 ∙ MPa −1 , which is ~ 2–10 times higher than that of other membranes with similar rejections. The PEI/PAA membranes are capable of retaining > 96.1% of negatively charged dyes following the mechanism of electrostatic repulsion. We demonstrate that the membranes can also separate positively and neutrally charged dyes from water via other mechanisms. This work opens a new avenue for the design and preparation of high-flux NF membranes, which is also applicable to enhance the permeance of other TFC membranes.
研究领域:
材料科学
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纳米技术
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膜技术
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2024
·
IF 6.7
Chang Li,Kang Tan,Fangzheng Hua,Changxue Wang,Xiaolin Wang,Fei Wang,Mengting Sun
Hydrate-based natural gas storage and transportation is in urgent need of efficient and eco-friendly promotion methods. Here, we employed a kind of eco-friendly hydrogels, which were polyvinyl alcohol-co-acrylic acid (PVA-co-PAA), as carriers for trace SDS solution to construct a co-promoter for methane hydrate formation. Under static condition without stirring, trace SDS solution that was lower than or equal to 0.4 mmol L −1 had no or had extremely weak promoting effects on hydrate formation kinetics, whereas hydrogel-carried trace SDS exhibited excellent synergistic promoting effects. The HS-0.4 under a SDS concentration of 0.4 mmol L −1 and at a swelling ratio of 200 g g −1 achieved the most remarkable promotion, and the induction time was 0 min, the hydrate growth rate at t 90 was 0.1552 ± 0.0068 mmol mL −1 min −1 , the methane uptake was 121.70 ± 1.58 mmol mol −1 and the water-to-hydrate conversion was 0.730 ± 0.009. During synergistic promotion process, on the one hand, hydrogels keeping with large surface area increased gas–liquid interface, and were in charge of facilitating hydrate nucleation and shortening induction time; on the other hand, trace SDS produced strong capillary effect and sharply boosted mass transfer during hydrate growth period, which drastically enhanced hydrate growth rate and methane uptake. Morphology observation on hydrate evolutions unveiled that the co-promoter significantly improved poor hydrate compactness and partly relieved foam problem caused by SDS. Hydrogel-carried trace SDS can serve as an effective, eco-friendly and economical promotion approach for methane hydrate formation, which might be of great benefit for hydrate-based natural gas storage and transportation.
研究领域:
化学工程
·
材料科学
·
环境科学
·
能源存储与运输
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Mochou Gao,Chenglin Xu,Jinyu Deng,Tong Zhu,Zhilong Xie,Xueyan Zou,Wensheng Yang
Chitosan (CS) was deposited onto the surface of Fe 3 O 4 nanoparticles by anionic cross-linking to obtain Fe 3 O 4 @CS nanocomposite with improved biocompatibility ascribed to CS. The optimized reaction condition for preparing Fe 3 O 4 @CS was established. Furthermore, polyacrylic acid (PAA) was grafted onto the surface of Fe 3 O 4 @CS to obtain magnetically responsive Fe 3 O 4 @CS/PAA nanocomposite; and dextrin was grafted onto the surface of Fe 3 O 4 @CS/PAA by hydrogen bonding to obtain Fe 3 O 4 @CS/dextrin nanoadsorbent with desired magnetic response for the rapid purification of the maltose binding protein-tagged (MBP-tagged) fusion proteins from Escherichia coli lysate. The as-obtained Fe 3 O 4 @CS/PAA nanocomposite was loaded with relevant antibodies to construct the platform for the purification of MBP-tagged, glutathione S-transferase-tagged, and histidine-tagged fusion proteins. Findings demonstrate that multi-tagged proteins platform exhibits rapid recognition and high binding abilities as well as desired specification, universality, and recycling ability to the tested tagged fusion proteins, because they can strongly load different protein ligands.
研究领域:
纳米技术
·
生物技术
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）