ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xiaojian Xu,Yuhe Shen,Ruizhe Xing,Jie Kong,Rongxin Su,Renliang Huang,Wei Qi
The development of high-performance biosensors is a key focus in the nanozyme field, but the current limitations in biocompatibility and recyclability hinder their broader applications. Herein, we address these challenges by constructing core–shell nanohybrids with biocompatible poly(ethylene glycol) (PEG) modification using a galvanic replacement reaction between orthovanadate ions and liquid metal (LM) (VOx@EGaIn-PEG). By leveraging the excellent charge transfer properties and the low band gap of the LM surface oxide, the VOx@EGaIn-PEG heterojunction can effectively convert hydrogen peroxide into hydroxyl radicals, demonstrating excellent peroxidase-like activity and stability (Km = 490 μM, vmax = 1.206 μM/s). The unique self-healing characteristics of LM further enable the recovery and regeneration of VOx@EGaIn-PEG nanozymes, thereby significantly reducing the cost of biological detection. Building upon this, we developed a nanozyme colorimetric sensor suitable for biological systems and integrated it with a smartphone to create an efficient quantitative detection platform. This platform allows for the convenient and sensitive detection of glucose in serum samples, exhibiting a good linear relationship in the range of 10–500 μM and a detection limit of 2.35 μM. The remarkable catalytic potential of LM, combined with its biocompatibility and regenerative properties, offers valuable insights for applications in catalysis and biomedical fields.
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 5
·753-762
·
IF 4.776
Zheng Ruan,Yangyang Zhao,Pan Yuan,Le Liu,Yucai Wang,Lifeng Yan
Drug-free biocompatible nanoparticles with photosensitizers as both diagnostic and therapy agents represent an emerging approach for imaging-guided photodynamic therapy (PDT). Here, a NSH-active boron-dipyrromethene (BODIPY) derivative with a bromine substituted (BODIPY-Br2, for simplicity, BDP-Br) photosensitizer was synthesized; it showed a high ability of generating reactive oxygen species (ROS) upon irradiation for PDT and near infrared fluorescence imaging ability. Then, a simple PEGylated BDP-Br (PEG-BDP) as a kind of macro photosensitizer was prepared which showed superior cellular uptake ability, high efficiency of imaging, and curing with PDT therapy in vitro and in vivo. After administration with a 4T1 orthotopic tumor model, the resultant PEG-BDP showed prolonged blood circulation and preferential tumor accumulation compared with free BDP-Br molecules. Once accumulated in tumor tissues, therapeutic effects of PEG-BDP could significantly suppress primary tumor growth and has no evident side effects at a weak irradiation energy as low as 35 mW cm−2 for 20 minutes. The designation of this simple structure macro photosensitizer agent provides new avenues for efficient imaging-guided cancer PDT.
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PubMed
高影响因子
Small
·
2024
·
IF 13
Shiyan Fu,Yong Li,Li Shen,Yonglai Chen,Jingxuan Lu,Yonghong Ran,Yazhen Zhao,Hong Tang,Longfei Tan,Qinyang Lin,Yuhui Hao
Unavoidable damage to normal tissues and tumor microenvironment (TME) resistance make it challenging to eradicate breast carcinoma through radiotherapy. Therefore, it is urgent to develop radiotherapy sensitizers that can effectively reduce radiation doses and reverse the suppressive TME. Here, a novel biomimetic PEGylated Cu 2 WS 4 nanozyme (CWP) with multiple enzymatic activities is synthesized by the sacrificing template method to have physical radiosensitization and biocatalyzer-responsive effects on the TME. Experiment results show that CWP can improve the damage efficiency of radiotherapy on breast cancer cell 4T1 through its large X-ray attenuation coefficient of tungsten and nucleus-penetrating capacity. CWP also exhibit strong Fenton-like reactions that produced abundant ROS and GSH oxidase-like activity decreasing GSH. This destruction of redox balance further promotes the effectiveness of radiotherapy. Transcriptome sequencing reveals that CWP induced ferroptosis by regulating the KEAP1/NRF2/HMOX1/GPX4 molecules. Therefore, owing to its multiple enzymatic activities, high-atomic W elements, nucleus-penetrating, and ferroptosis-inducing capacities, CWP effectively improves the efficiency of radiotherapy for breast carcinoma in vitro and in vivo. Furthermore, CWP-mediated radiosensitization can trigger immunogenic cell death (ICD) to improve the anti-PD-L1 treatments to inhibit the growth of primary and distant tumors effectively. These results indicate that CWP is a multifunctional nano-sensitizers for radiotherapy and immunotherapy.
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PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2024
·
IF 5.9
Weixiong Dong,Xulei Cao,Haiyan Tao,Hongrui Jiang,Yaoyao Wang,Guihua Meng,Shuping Jia
To address the issue of current solar thermal conversion materials being unable to function continuously in the presence of intermittent solar radiation. The phase-change energy storage material polyethylene glycol (PEG) is ingeniously introduced into the interfacial evaporation material. Polyacrylic acid (PAA) hydrogel is employed as the leakproof support material to stabilize the form of PEG. Graphene oxide (GO) and nano silver (Ag NPs) are utilized as the solar thermal conversion medium. A novel interface evaporation three-dimensional network gel (PEG/PAA/GO-Ag) composite PCM was constructed by sol–gel-in-situ reduction method. In the full spectrum range, there is a good match between the sunlight’s spectrum and a light absorption rate of 95.4 %. At 1 kW·m −2 light, an evaporation rate of 2.14 kg·m −2 ·h −1 and a photothermal conversion efficiency of 91.2 % were achieved. In the absence of light, the stored thermal energy is released through the phase transition process and continues to evaporate clean water, resulting in an evaporation rate of 0.73 kg·m −2 ·h −1 and a photothermal conversion efficiency of 35.0 %. Furthermore, the material demonstrates excellent performance stability in 10 consecutive heating and cooling cycle evaporation tests. It exhibits good cyclic stability and can be utilized for saltwater desalination and dye sewage treatment.
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高影响因子
LANGMUIR
·
2024
·
IF 3.7
Chunyang Liu,Yanrong Cheng,Delan Ge,Ju Xie,Xia Guo
Aqueous two-phase systems (ATPS) are considered a new type of green separation system, but their low selectivity to target molecules limits their practical application. In this study, we used surfactant@MWCNT as an adjuvant to improve the selectivity of PEG/(NH4)2SO4 ATPS. The surfactants used include Triton X-100 (TX-100), Tween 80, sodium dodecyl sulfate (SDS), sodium dodecyl benzenesulfonate (SDBS), and bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT). Nine aromatic compounds, including syringic acid, vanillin, tryptophan, phenylalanine, tyrosine, mandelic acid, theophylline, theobromine, and cephalexin, were selected as model extracts. The results showed that the use of the surfactant@MWCNT composite as an adjuvant significantly improved the selectivity of the ATPS toward syringic acid among the nine extracts. Moreover, the selectivity of the ATPS for syringic acid with the TX-100/MWCNT composite as the adjuvant is better than that with the Tween 80/MWCNT composite as the adjuvant, followed by that with the anionic surfactant/MWCNT composite as the adjuvant. The enhanced selectivity of ATPS by the surfactant/MWCNT composite is ascribed to the spatial effect and the electrostatic and hydrophobic interactions between the extract and the surfactant adsorbed on the MWCNT.
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PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2024
·
IF 4.4
Han Song,Jiasheng Fan,Shengkai Liu,Boyu Chen,Jin Huang,Yujie Fu,Zhiguo Liu
In this study, we synthesized DOX-loaded H-ZIF-8@CaCO 3 -PEG nanocarrier, which combined with calcium overload and photothermal therapy (PTT) for synergistic therapy. The nanocarrier comprised a PEG modified calcium carbonate (CaCO 3 ) shell and a hollow imidazolate zeolite skeleton (H-ZIF-8) core, in which the chemotherapy drug doxorubicin hydrochloride (DOX) and photosensitizer indocyanine green (ICG) were simultaneously co-loaded. In vitro cellular experiments revealed that the lethality of DOX&ICG@H-ZIF-8@CaCO 3 -PEG against tumor cells was significantly higher than that of the single chemotherapy owing to the combination with chemotherapy and photothermal therapy as well as calcium overloading. This drug-loaded nanocarrier exhibits notable characteristics such as exceptional stability, elevated drug loading capacity and efficient release of the loaded drug, which provides a promising perspective for efficient synergistic therapy.
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高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2016
·Vol. 16
·期 7
·7530-7538
·
IF 1.338
Wang Yang,Nie Jinshan,Wang Wei,Gu Zhun
In this work, redox and pH responsive vesicles of PEGylated hyperbranched poly(amidoamine)-doxorubicin (PPCD) conjugates were prepared for the improvement of cancer therapy. The PPCD 3/1 and PPCD 6/1 conjugates were respectively prepared by the acid sensitive cis-aconityl linkage
between doxorubicin and differently PEGylated hPAMAM (PEG3-g-hPAMAM and PEG6-g-hPAMAM). The PPCD vesicles were prepared by disulfide-crosslinking the micelles formed in THF and then transferring them from THF into water. The crosslinked
PPCD vesicle with a higher PEGylated degree showed a measurable decrease in size and zeta potential. Both the crosslinked PPCD vesicles could be disassembled into small-sized conjugates under the redox condition. The drug release profiles showed that the both vesicles presented stimuli-triggered
drug release in acidic and reducing environment and lower DOX leakage under neutral condition. The cytotoxicity assay reflected that both the PPCD vesicles could effectively kill the gastric cancer MGC803 cells, and PPCD 6/1 vesicle with smaller size presented higher cytotoxicity than PPCD
3/1 vesicle. Considering the more applicable size (about 100 nm), higher capacity of killing tumor cells and better compatibility, the PPCD-6 vesicle with higher PEGylation degree is more favorable to promote the therapeutic effect of cancer, and will be more suitable as drug delivery system
for solid tumor treatment.
研究领域:
药剂学
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肿瘤学
·
生物材料学
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DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 8
·2001-2009
·
IF 3.641
Ma Jun,Yu Da-Hai,Zhao Di,Huang Teng,Dong Min,Wang Ting,Yin Hai-Tao
Radiotherapy is an effective anti-cancer therapy for patients with non-small cell lung cancer (NSCLC), however, the prognosis is unsatisfactory owing to radio-resistance and toxicity. It is crucial to improve radiotherapy efficacy. Ag nanoparticles (NPs) and ginsenoside Rg3 (Rg3) exerted
antitumor and radio-sensitization effects. Therefore, we investigated whether poly-lactide-co-glycolide-polyethylene glycol (PLGA-PEG)-Rg3-Ag will function as a noninvasive, tracing, radiotherapy sensitizer. The morphology of NPs was visualized with transmission electron microscopy (TEM).
The drug loading content, encapsulation efficiency, and cumulative drug release of Rg3 was determined by HPLC. Cellular uptake of NPs in A549 and SPCA-1 was measured by immunostaining. The radio-sensitization effect of PLGA-PEG-Rg3-Ag in vitro was determined in A549 by detecting proliferation,
colony formation, and apoptosis with CCK-8, clonogenic survival assay, and flow cytometry, while in vivo was determined in nude mice by testing the body weight and tumor volume. PLGA-PEG-Rg3-Ag exerted radio-sensitization effect by reducing cell proliferation and colony formation while
enhancing cell apoptosis in A549; reduced tumor volume in nude mice. PLGA-PEG-Rg3-Ag exhibits radio-sensitization effects in NSCLC.
研究领域:
肿瘤学
·
药剂学
·
放射治疗学
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DOI
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 41
·24624-24630
·
IF 3.119
Gaizhen Kuang,Qingfei Zhang,Shasha He,Ying Liu
Curcumin (Cur) can be used as a photosensitizer in the photodynamic therapy (PDT) of cancer, but its low bioavailability limits further clinical application. A mesoporous silica-based drug delivery system (PEGylated mesoporous silica nanoparticles, MSN-PEG@Cur) was designed to solve the problem. The successful preparation of MSN-PEG@Cur was characterized by several physico-chemistry techniques. The endocytosis, ROS generation and in vitro anti-cancer efficacy of MSN-PEG@Cur were evaluated in detail step by step. The results indicated that MSN-PEG@Cur could be effectively endocytosed into cells and release Cur, which can promptly generate ROS upon irradiation, achieving effective PDT in cancer treatment. This MSNs-based drug delivery system provides an alternative strategy for Cur loading and PDT of cancer.
研究领域:
药学
·
纳米医学
·
肿瘤学
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DOI
高影响因子
POLYMER
·
2011
·
IF 3.829
Tian-Bin Ren,Wen-Juan Xia,Hai-Qing Dong,Yong-Yong Li
A novel reduction-sensitive sheddable micelle based on disulfide-linked hybrid PEG-polypeptide mPEG-SS-Pleu was demonstrated for intracellular drug delivery . These micelles are composed of an mPEG shell and polypeptide core, characterized by FT-IR, 1 H NMR, fluorescence techniques, TEM , and DLS . Interestingly, they would undergo a fast sheddable process when encounter the reduction sensitive condition, indicated by the aggregation phenomena in the presence of DTT, a reduction agent, which could cleave the disulfide bond between the micellar core and shell and consequently leading to the aggregation of hydrophobic core. Cytotoxicity study revealed that copolymers in this system have good biocompatibility and their self-assembled micelles showed a high drug loading efficiency for DOX , a hydrophobic drug model, and released DOX quantitatively in response to the intracellular level of reducing potential. Cellular uptake experiments demonstrated that the fluorescently labeled micelles could be successfully internalized into human liver carcinoma HepG2 cells, evidenced by confocal laser scanning microscopy . Above results indicate that the copolymers may have great potential in drug delivery to achieve improved cancer therapy.
研究领域:
药剂学
·
生物材料学
·
肿瘤学
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DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2020
·
IF 2.734
Bo Yu,Ying Pu,Jun Liu,Jie Liao,Ke Chen,Jiani Zhang,Wen Zhong,Yalan Hu,Xue-Qiang Wang,Bo Liu,Huixia Liu,Weihong Tan
Targeted delivery of antiobesity drugs to adipocytes presents a novel strategy for obesity treatment. The 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in adipose tissue is an attractive therapeutic target of obesity. Emodin (EMO) has been proven to be a potent and selective inhibitor of 11β-HSD1, but it frequently exerts low bioavailability owing to its poor water solubility and lack of tissue specificity. In this work, we conjugated a nanoscale drug delivery polymer with Adipo8 (Ap), a DNA aptamer with high affinity to mature white adipocytes, as a targeting modality that enabled selective delivery of emodin to adipocytes. Emodin-loaded PEG-PLGA nanoparticles (EMO-NPs) were formulated by a modified oil-in-water (O/W) emulsion solvent evaporation method, and Ap was covalently bound to the NP surface via the EDC/NHS method. The NPs were structurally investigated by TEM imaging, DLS and UV-VIS spectroscopy. The resulting aptamer-conjugated, emodin-loaded nanoparticles (Ap-EMO-NPs) had a spherical shape and an average particle size of 146.7 ± 27.85 nm with a drug loading of around 6.8% and a sustained-release property. Confocal microscopy and flow cytometry demonstrated a significant increase in the internalization of Ap-EMO-NP in differentiated 3T3-L1 cells compared to EMO-NP functionalized with nonspecific aptamer. As confirmed by Oil Red O coloring, the Ap-EMO-NPs reduced lipid stacking in 3T3-L1 adipocytes in a sustained-release and dose-dependent manner. The results indicated that Adipo8-functionalized PEG-PLGA NPs could be a potential targeted therapeutic delivery vehicle for obesity treatment.
研究领域:
药理学
·
生物医学工程
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Jun-Jun Chen,Ying Li,Xu-Min Zheng,Fu-An He,Kwok-Ho Lam
In this work, polyethylene glycol grafted graphene (PEG-graphene) was obtained from the amidation reaction between graphene oxide and methoxypolyethylene glycol amine followed by the NaHB 4 reduction and characterized by transmission electron microscope, Fourier transform infrared spectroscope, wide-angle X-ray diffraction, Raman spectroscope, X-ray photoelectron spectroscope, and thermogravimetric analysis. The PEG-graphene was incorporated into polyvinylidene fluoride (PVDF) to form novel PEG-graphene/PVDF composites by the solution blending method. The well dispersion of PEG-graphene in the PEG-graphene/PVDF composite was confirmed by scanning electron microscopy. Based on the results of FTIR, WAXD, differential scanning calorimetry, and XPS, it was found that the presence of PEG-graphene effectively enhanced the electroactive crystalline content of PVDF from 24.6% for the pure PVDF to 90.5% for the PEG-graphene (15 wt.%)/PVDF composite by the interfacial interaction. Moreover, the PEG-graphene (10 wt.%)/PVDF composite near the percolation threshold possessed a much higher dielectric constant of 53.3 compared to the pure PVDF (8.2), and a relatively low dielectric loss of 0.265 at 1000 Hz.
研究领域:
材料科学
·
高分子化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）