PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Xin-Yu Ji,Bing-Yi Wang,Yi-Feng Zhang,Yu-Jing Zhang,Ya-Jie Lai,Yang Yang,Xiang-Chen Wang,Su-Yan Wang,Pedro Laborda,Xin-Chi Shi
BACKGROUND Epicoccum sorghinum is a pathogenic fungus that causes leaf spot in a wide range of plants, including maize, and synthesizes the mycotoxin tenuazonic acid (TEA), which is carcinogenic. Despite the relevant economic and yield losses caused by E. sorghinum worldwide, methods for the control of this pathogen are lacking. RESULTS In this work, the efficacy of Bacillus -produced dipicolinic acid (DPA) for control of E. sorghinum was evaluated using in vitro and in vivo assays, and compared with the efficacy of three commercial fungicides, including carbendazim, prochloraz, and thiram. DPA inhibited E. sorghinum mycelial growth, and conidia germination, and produced important alterations in E. sorghinum hyphae. Interestingly, 10 mM DPA reduced TEA biosynthesis by 86.6%. Although DPA rapidly degraded on maize leaves, 10 mM DPA showed higher preventive (67.4% lesion length inhibition) and inhibitory (89.5% lesion length inhibition) efficacies for the control of E. sorghinum on maize leaves compared to the commercial fungicides. CONCLUSIONS Collectively, this study presents the first method for the control of E. sorghinum on maize and demonstrates that DPA application is a suitable approach to inhibit E. sorghinum symptoms in plants and TEA biosynthesis. © 2024 Society of Chemical Industry.
研究领域:
植物病理学
·
农业科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Shuning Chen,Ao Yuan,Huafeng Wang,Daibin Yang,Li Cui,Fengjiao Hao,Xiaojing Yan,Huizhu Yuan
BACKGROUND Citrus huanglongbing (HLB) is a devastating disease in citrus, caused by Candidatus Liberibacter asiaticus (CLas), which primarily resides in the phloem where chemicals cannot effectively reach, posing a significant challenge in controlling HLB. To address these challenges, plant essential oils (EOs), widely used as transdermal enhancers and known for their benefits for plant tissues, were investigated for their potential to enhance chemical permeation. RESULTS In this study, seven EOs – eugenol, carvacrol, eucalyptol, geraniol, linalool, cinnamaldehyde, and d -limonene – were evaluated for their potential to enhance chemical penetration into citrus leaves. Preliminary screening with tracer methods revealed that geraniol provided the greatest enhancement of permeation, achieving the deepest and broadest distribution into the spongy tissue of the citrus leaf. The permeation levels of rhodamine B and crystal violet through the cuticular layer were elevated from 0.55 mg L −1 and 0.11 mg L −1 to 20.04 mg L −1 and 7.14 mg L −1 , respectively, by the addition of geraniol. Further validation through high-performance liquid chromatography–mass spectrometry (HPLC–MS) confirmed that the penetration of fludioxonil and tetracycline into citrus leaf tissues was enhanced by 15.07-fold and 20.43-fold, respectively. In planta experiments confirmed that adding geraniol to tetracycline and oxytetracycline via foliar application eliminated CLas bacteria in HLB-affected citrus more efficiently than controls. CONCLUSION Our study underscores the potential of geraniol as an effective EO-based permeation enhancer for combating HLB. By significantly improving the delivery and efficacy of bactericidal treatments, geraniol offers a promising strategy for managing this catastrophic citrus disease, potentially transforming the approach to controlling HLB. © 2024 Society of Chemical Industry.
研究领域:
植物病理学
·
农业化学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Ya Wang,Renfeng Zhang,Xiaoqiu Guo,Ying Xu,Wei Sun,Shengxin Guo,Jian Wu
BACKGROUND Crops are consistently under siege by a multitude of pathogens. These pathogenic microorganisms, including viruses and bacteria, result in substantial reductions in quality and yield globally by inducing detrimental crop diseases, thus posing a significant challenge to global food security. However, the biological activity sepectrum of commercially available pesticides is limited and the pesticide efficacy is poor, necessitating the urgent development of broad-spectrum and efficient strategies for crop disease prevention and control. RESULTS The bioassay results revealed that certain target compounds demonstrated outstanding in vivo antiviral efficacy against cucumber mosaic virus and tobacco mosaic virus. In particular, compound D6 showed remarkable antiviral activity against CMV, significantly higher than that of the control agent ningnanmycin. Mechanism of action studies have shown that compound D6 could enhance the activity of defense enzymes and upregulate the expression of genes related to disease resistance, thereby enhancing the antiviral effects in plants. In addition, these compounds displayed superior inhibitory activity against plant bacterial diseases. For Xoo , compound D10 showed an excellent inhibitory effect that was better than that of the control agent bismerthiazol. Scanning electron microscopy and fluorescence double-staining experiments revealed that compound D10 effectively inhibited bacterial growth by disrupting the cell membrane. CONCLUSION A series of trifluoromethyl hydrazone derivatives were designed and synthesized, and it was found that they have control effects on plant viruses and bacterial diseases. In addition, this study revealed the mechanism of action of the active compounds and demonstrated their potential as multifunctional crop protectants. © 2024 Society of Chemical Industry.
研究领域:
农业科学
·
植物病理学
·
药物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·Vol. 80
·期 3
·1076-1086
·
IF 4.1
Shunyu Xiang,Xiaofeng Zhang,Zhe Cao,Shiqi Peng,Jingyun Xu,Qianqiao Huang,Jin Huang,Chen Xu,Xianchao Sun
BACKGROUND In recent years, nanomaterials-based pesticide carriers have garnered significant attention and sparked extensive research. However, most studies have primarily focused on investigating the impact of physical properties of nanomaterials, such as size and modifiable sites, on drug delivery efficiency of nano-pesticides. The limited exploration of biologically active nanomaterials poses a significant obstacle to the advancement and widespread adoption of nano-pesticides. In this study, we prepared chitin nanocrystals (ChNC) based on acid hydrolysis and systematically investigated the differences between nano- and normal chitin against plant bacteria ( Pseudomonas syringae pv. tabaci ). The primary objective was to seek out nanocarriers with heightened biological activity for the synthesis of nano-pesticides. RESULTS Zeta potential analysis, Fourier Transform infrared spectrometry (FTIR), X-Ray diffraction (XRD), Atomic force microscopy (AFM) and Transmission electron microscopy (TEM) identified the successful synthesis of ChNC. ChNC showcased remarkable bactericidal activity at comparable concentrations, surpassing that of chitin, particularly in its ability to inhibit bacterial biofilm formation. Furthermore, ChNC displayed heightened effectiveness in disrupting bacterial cell membranes, resulting in the leakage of bacterial cell contents, structural DNA damage, and impairment of DNA replication. Lastly, potting experiments revealed that ChNC is notably more effective in inhibiting the spread and propagation of bacteria on plant leaves. CONCLUSION ChNC exhibited higher antibacterial activity compared to chitin, enabling efficient control of plant bacterial diseases through enhanced interaction with bacteria. These findings offer compelling evidence of ChNC's superior bacterial inhibition capabilities, underscoring its potential as a promising nanocarrier for nano-pesticide research. © 2023 Society of Chemical Industry.
研究领域:
农业科学
·
材料科学
·
微生物学
✓ 已验证
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2021
·Vol. 77
·期 5
·2576-2583
·
IF 4.845
Wei Deng,Mengting Yang,Yang Li,Zhiming Xia,Yongrui Chen,Shuzhong Yuan,Qian Yang
BACKGROUND Chinese sprangletop ( Leptochloa chinensis (L.) Nees) is one of main grass weeds invading Chinese rice fields. The target-site resistance (TSR) of cyhalofop-butyl have been widely reported in L. chinensis populations, but the non-target-site resistance (NTSR) mechanisms have not yet been well-characterized. This study aims to investigate the likely NTSR in a cyhalofop-butyl-resistant L. chinensis population (YZ-R), which was collected from Yangzhou city, Jiangsu Province, China. RESULTS Dose–response assays showed the YZ-R population exhibited 191.6-fold resistance to cyhalofop-butyl, compared to the susceptible population (YZ-S). This resistance is not target-site based, because no mutations in the two ACCase genes were detected in the YZ-R plants compared to the YZ-S plants, and the ACCase genes expression levels were similar in YZ-S and YZ-R plants. In addition, the cytochrome P450 inhibitor malathion and piperonyl butoxide (PBO), and glutathione S-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) did not significantly reverse cyhalofop-butyl resistance in the YZ-R population. However, liquid chromatography−mass spectrometry (LC–MS) analysis indicated that the metabolic rates of cyhalofop acid in YZ-R plants was significantly faster (5 to 10- fold) than in YZ-S plants. Furthermore, the YZ-R population showed no cross-resistance to other ACCase-inhibiting herbicides. CONCLUSION These results indicated that cyhalofop-butyl resistance in the YZ-R population is due to non-target-site based enhanced herbicide metabolism. Resistance in this population is likely involved in a specific detoxification enzyme, with possible high catalytic efficiency and strong substrate specificity, therefore leading to high-level and single resistance to cyhalofop-butyl. © 2021 Society of Chemical Industry
研究领域:
植物学
·
农业科学
·
分子生物学
✓ 已验证
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·
IF 4.1
Qichang Liu,Mei Zuo,Yi Song,Shuzhong He,Jian Huang,Yang Chen
BACKGROUND Phytopathogens cause various diseases by parasitizing crops, reducing crop yield and resulting in substantial economic losses in agricultural production. A novel type isolated from the perennial herbaceous Peganum harmala L. seeds, β -carboline alkaloids pegaharine A ( PA ), has become a hot topic in developing plant-originated green pesticides owing to their significant physiological activities. RESULTS A scalable bioinspired total synthesis of PA is accomplished in the present work. The systematical biological assay study showed that PA exhibited moderate inhibitory activity against nine tested plant pathogenic fungi and showed significant inhibitory activity in vitro against the three tested plant pathogenic bacteria. Most noteworthy is the inhibitory rates of PA on Xanthomonas oryzae pv. oryzae ( Xoo ), X. oryzae pv. oryzicola ( Xoc ) and X. axonopodis pv. citri ( Xac ) of 93.6%, 92.1% and 86.1%, respectively, which are better than the control drug, bismerthiazol (63.4%, 61.2% and 53.7% at 100 μg mL −1 concentration). Furthermore, the EC 50 value of PA against Xoo , Xoc and Xac was 52.2, 60.0 and 65.1 μg mL −1 , respectively, superior to 72.9, 64.2 and 70.1 μg mL −1 of the control drug. Moreover, the anti- Xoo mechanistic studies revealed that PA exerted its antibacterial effects by increasing the permeability of the bacterial membrane, reducing the extracellular polysaccharide content and inducing morphological changes in bacterial cells. CONCLUSION A novel β -carboline alkaloid, PA , was prepared by biomimetic total synthesis. Its significant antibacterial activity was closely related to the permeation of bacterial cell membranes, which was confirmed by anti- Xoo mechanistic studies. More importantly, the structure could be regarded as a model for developing novel bactericides. © 2023 Society of Chemical Industry.
研究领域:
植物化学
·
植物病理学
·
有机合成
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2020
·Vol. 77
·期 3
·1383-1391
·
IF 3.75
Yang Zhou,Fei Fan,Li Wang,Chingchai Chaisiri,Liang-Fen Yin,Wei-Xiao Yin,Chao-Xi Luo
BACKGROUND Peach scab, caused by Venturia carpophila , can significantly reduce both the yield and quality of peach fruit. It is difficult to distinguish peach scab from other peach diseases such as black spot and bacterial spot . An efficient assay is needed to identify V. carpophila in order to develop scientific management strategies. RESULTS A set of loop-mediated isothermal amplification (LAMP) primers was designed based on the internal transcribed spacer (rDNA-ITS) sequence to detect V. carpophila . Compared with the conventional polymerase chain reaction (PCR) method, the LAMP method not only exhibited higher sensitivity and specificity in the detection of V. carpophila , but also required simpler equipment and less operational time. The minimum detectable concentration of V. carpophila genomic DNA with the LAMP method was 56.6 fg μl –1 , which was 100 times lower than with the conventional PCR method. When eight fungal species including V. carpophila (23 isolates from 14 provinces) and one bacterial species were used with LAMP detection, only V. carpophila showed a color change, from brown to yellowish green, and ladder-like bands in electrophoresis, indicating successful amplification. Moreover, when crude DNAs of peach fruit samples were used in LAMP detection, amplification was observed only from diseased fruits, and not from healthy fruits and the negative control. CONCLUSION The LAMP assay shows simplicity, rapidity, high sensitivity and specificity, and will be useful in distinguishing scab caused by Venturia carpophila from other diseases with similar symptoms. © 2020 Society of Chemical Industry
研究领域:
植物病理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 78
·期 6
·2240-2249
·
IF 4.462
Tan Dai,Zhiwen Wang,Xingkai Cheng,Huige Gao,Li Liang,Pengfei Liu,Xili Liu
BACKGROUND Fungicide resistance has become a serious problem for different mode of action groups except for uncouplers, which makes their resistance mechanism a hot topic, which until now, has not been well clarified. SYP-14288, a newly developed diarylamine fungicide modeled on fluazinam, has shown good toxicity to both oomycete and fungus by the action of uncoupling. In this research, the resistance of Phytophthora capsici to SYP-14288 was studied to clarify the resistance mechanism of uncouplers. RESULTS The toxicity tests of resistant strains against SYP-14288 showed multidrug resistance. The high-performance liquid chromatography (HPLC) results showed that resistant strains could efflux the fungicide, and this ability could be inhibited by the efflux pump inhibitor amitriptyline. The target protein of amitriptyline is P-glycoprotein (P-gp), which was overexpressed in resistant strains. Three products of nitrate reduction of SYP-14288 were detected and determined by HPLC-Q-TOF. Eight cytochrome P450 monooxygenase (P450) proteins were differentially involved in the reduction reaction. CONCLUSION Both fungicide efflux and detoxification metabolism were involved in the resistance mechanisms of P. capsici to SYP-14288. © 2022 Society of Chemical Industry.
研究领域:
植物病理学
·
分子生物学
·
杀菌剂科学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2019
·Vol. 75
·期 7
·2009-2019
·
IF 3.255
Bo Hu,He Huang,Qi Wei,Miaomiao Ren,David K Mburu,Xiangrui Tian,Jianya Su
BACKGROUND Glutathione S -transferases (GSTs) are a superfamily of multifunctional dimeric proteins existing in both prokaryotic and eukaryotic organisms. They are involved in the detoxification of both endogenous and exogenous electrophiles, including insecticides. However, the molecular mechanisms underlying the regulation of GST genes in insects are poorly understood. RESULTS We first identified at least three GST genes involved in resistance to the insecticides chlorpyrifos and cypermethrin. Analysis of upstream sequences revealed that three GSTs ( SeGSTo2 , SeGSTe6 and SeGSTd3 ) harbor the same cap ‘n’ collar C/muscle aponeurosis fibromatosis (CncC/Maf) binding site, and SeGSTo2 and SeGSTe6 contain the aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator (AhR/ARNT) binding site. Luciferase reporter assay showed co-transfection of reporter plasmid containing the SeGSTe6 promoter with CncC and/or Maf expressing constructs significantly boosted transcription. Similarly, AhR and/or ARNT expressing constructs also significantly increased the promoter activities. The co-transfection of mutated reporter plasmid with CncC/Maf or AhR/ARNT did not increase transcription activity anymore. Constitutive over-expression of CncC, Maf and AhR was also found in the HZ16 strain, which might be the molecular mechanism for up-regulated expression of multiple detoxification genes conferring resistance to insecticides. CONCLUSION These results suggest that CncC/Maf and AhR/ARNT coordinately regulate the expression of multiple GST genes involved in insecticide resistance in Spodoptera exigua . © 2019 Society of Chemical Industry
研究领域:
昆虫学
·
分子生物学
·
毒理学
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 78
·期 12
·5358-5365
·
IF 4.462
Jianfeng Liu,Yi Luo,Xuefeng Jiang,Guangjun Sun,Saijie Song,Maofa Yang,Jian Shen
Background Traditional abamectin (Abm) formulations have several shortcomings, such as low water solubility, burst release behavior, poor photostability, and short persistence periods, which decrease their pesticidal activity and the risks they pose to the environment. Nanomaterial-based pesticide delivery systems (PDSs) provide new strategies for the efficient and safe application of pesticides. Here, we developed Abm-loaded graphene oxide (Abm/GO) as a PDS for the sustained release of Abm, which shows enhanced control efficacy against Plutella xylostella . Results The hydrophobic Abm molecule was effectively loaded on GO nanocarrier by a physisorption method, which formed a uniform and stable Abm/GO nanoformulation. GO possesses high adsorption capacity and can effectively load Abm. The Abm/GO nanoformulation shows enhanced water dispersion stability and can remain stable during a 2-year storage period in contrast to the water-insoluble Abm. In addition, the Abm/GO nanoformulation exhibits sustained pesticide release behavior and possesses significantly improved anti-ultraviolet properties. Thus, the Abm/GO nanoformulation shows superior pesticidal activity compared with Abm. Abm/GO showed negligible toxicity to maize seedlings, and its GO nanocarrier can reduce the cytotoxicity of Abm to A549 cells. Conclusion GO-based PDSs can effectively overcome the disadvantages of traditional pesticides, such as their insolubility, burst release behavior, instability, and short persistence period. GO shows much future promise in agriculture in light of its industrialization potential. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
研究领域:
农业科学
·
害虫防治
·
纳米材料应用
✓ 已验证
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Meizi Wang,Guangyao Li,Lin Zhou,Youwu Hao,Longfei Wang,Xuewei Mao,Guoyan Zhang,Chenxiang Zhao
BACKGROUND Peanut southern blight disease, caused by Sclerotium rolfsii , is a destructive soil-borne fungal disease. The current control measures, which mainly employ succinate dehydrogenase inhibitors, are prone to resistance and toxicity to non-target organisms. As a result, it is necessary to explore the potential of eco-friendly fungicides for this disease. RESULTS Fourteen novel phthalide compounds incorporating amino acid moieties were designed and synthesized. The in vitro activity of analog A1 [half maximal effective concentration (EC 50 ) = 332.21 mg L −1 ] was slightly lower than that of polyoxin (EC 50 = 284.32 mg L −1 ). It was observed that on the seventh day, the curative activity of A1 at a concentration of 600.00 mg L −1 was 57.75%, while the curative activity of polyoxin at a concentration of 300.00 mg L −1 was 42.55%. These results suggested that our compound exhibited in vivo activity. Peanut plants treated with A1 showed significant agronomic improvements compared to the untreated control. Several compounds in this series exhibited superior root absorption and conduction in comparison to the endothermic fungicide thifluzamide. The growth promotion and absorption-conduction experiments demonstrated the reason for the superior in vivo activity of the target compound. Cytotoxic assays have demonstrated that this series of targeted compounds exhibit low toxicity levels toward human lo2 liver cells. CONCLUSION Our results provide a new strategy for the design and synthesis of novel green compounds. Furthermore, the target compound A1 can serve as a lead for further development of green fungicides. © 2024 Society of Chemical Industry.
研究领域:
植物病理学
·
农药化学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2021
·Vol. 78
·期 1
·43-51
·
IF 4.845
Xiaojing Yan,Huizhu Yuan,Yixuan Chen,Xin Shi,Xiaohui Liu,Zhenying Wang,Yue Liu,Daibin Yang
BACKGROUND The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), is an invasive pest. Maize is one of its preferred host plants. The traditional application of granules has been effective for the control of FAW. Nevertheless, the challenge is to develop an application method that is less labor-intensive and also efficient. RESULTS Tiny granules in the size range of 0.38–0.55 mm were prepared by the centrifugal granulation method for drone application. The in-swath distribution patterns of granules deposited on sticky tapes were similar to the distribution of aqueous droplets through drone spraying. In the range of 1.5–3.5 m, a relatively higher aircraft height of flight can provide a more uniform distribution pattern of deposited granules, but resulted in less deposit of tiny granules in the whorl of maize plants. Similarly, the deposit of granules in the whorl of maize plants significantly decreased when the flight speed increased from 4 to 6 m s −1 . Granules gathered in the whorl accounted for 50.8–58.7% of total granules deposited in the maize canopy. The field experiment demonstrates granular formulations (containing 0.25% chlorantraniliprole + 0.15% emamectin benzoate, or higher concentrations) can provide better and longer duration of FAW control than the aqueous spray formulation at the whorl stage of maize plants. CONCLUSION Broadcasting of tiny granules by drone can provide better FAW control than liquid spray. The architecture of the maize plant and the downward airstream of the drone create favorable conditions for the deposition of granules in whorls. © 2021 Society of Chemical Industry.
研究领域:
农业昆虫学
·
害虫防治
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）