Science Advances
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2026
·Vol. 12
·期 32
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IF 13.9
Zhikun Wu,Ziyue Meng,Wei Huang,Zhaoxiao Yu,Longtao Cao,Jie Wang,Zhipeng Xie,Meng Cui,Luyan Z. Ma,Feng Long
Resistance–nodulation–cell division (RND) efflux pumps are the major cause of multidrug resistance in Pseudomonas aeruginosa . The inner membrane protein MexK from P. aeruginosa is a narrow-spectrum RND transporter. It assembles with membrane fusion protein MexJ and outer membrane proteins to form a tripartite efflux complex that efficiently exports drugs like triclosan. Here, we resolve the cryo-EM structures of apo-MexK at 3.4-angstrom resolution and the triclosan-bound MexJK complex at 2.6-angstrom resolution. Besides the unique architectural features of MexK, our structural data reveal a triclosan-binding pocket within the transmembrane domain of MexK protomers, distinct from known substrate-binding sites in other RND transporters. Using molecular dynamics simulations and mutagenesis, we elucidate a previously uncharacterized triclosan transport tunnel across the inner membrane with cytosolic access. The key residues for triclosan efflux are further shown essential for two other potential substrates of MexK, chloramphenicol and pyrimethamine. Our results provide the molecular basis for an unusual drug trafficking pathway in RND efflux pumps.
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BIOLOGICAL CONTROL
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2026
·
IF 4
Lingling Cao,Junjie Lu,Yinglu Chen,Yajie Du,Shuqiong Yang,Zhiqiang Yan,Qingling Zheng,Pengfei Duan
White mold disease caused by Pseudodiploöspora longispora is destructive to the production and quality of Morchella . In this study, an endophytic bacterial strain YX-3 was isolated from the ascocarp of Morchella and exhibited significantly antagonistic effects against P. longispora with the inhibition rate of 91.5%. The strain YX-3 was identified to be Pseudomonas chlororaphis subsp. piscium through multi-locus sequence analysis and morphological characteristics. The gene features and functional annotation were analyzed according to the whole genome sequencing. The VOCs produced by strain YX-3 demonstrated significant inhibitory effects on mycelial growth, spore germination of P. longispora and reduced the severity of white mold disease through fumigation during storage. In field experiments, the biological control effect of YX-3 reached to 57.3%. The scanning and transmission electron microscope (SEM and TEM) and fluorescence microscopy revealed that VOCs from YX-3 caused abnormal changes and damaged the cell membrane integrity. Ten VOCs produced by YX-3 were detected through gas chromatography-mass spectrometry, of which seven were evaluated for their antifungal activity against P. longispora. The volatiles, namely 2-undecanone and 2-undecanol exhibited notable inhibitory effects on P. longispora with inhibition rate of 100%, 77.06% at 80 μL/plate and the minimum inhibition concentration of 2-nonanol was 0.75 μL/plate and reduced the severity of white mold disease. Overall, these findings elucidate the biocontrol mechanisms of P. chlororaphis subsp. piscium strain YX-3 and support its potential application as a biocontrol agent against white mold disease in Morchella .
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ACS Applied Bio Materials
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2026
·
IF 5.6
Lu Sheng,Wei Xiaoqian,Jiang Siyu,Wang Danqi,Liu Xiao,Hu Yufeng,Chen Ying,Ma Hang,Ding Ping,Kai Tianhan
Pomegranate ( Punica granatum ) peel is a rich source of polyphenols with recognized bioactivities. This study evaluated the quorum sensing (QS)-inhibitory and anti-biofilm properties of a standardized pomegranate extract, as well as its major ellagitannins (punicalagin and ellagic acid), against Pseudomonas aeruginosa , an important opportunistic pathogen associated with multidrug-resistant infections. Using the P. aeruginosa strain (ATCC 27853) and the QS biosensor Chromobacterium violaceum , the pomegranate extract and punicalagin (MIC = 0.5 and 0.1 mg/mL, respectively) suppressed the production of QS-regulated virulence factors (pyocyanin >80%, rhamnolipids >60%), inhibited bacterial motility, and disrupted biofilm formation (>70%) without inducing resistance. Cytotoxicity assays demonstrated the favorable cytocompatibility of the extract in mammalian cells. To enable localized delivery, a mechanically robust polyvinyl alcohol–chitosan (PVA–CS) hydrogel was developed. Structural characterization revealed a porous, hydrogen-bond-stabilized structure with enhanced thermal stability, favorable swelling behavior, and excellent moisture retention. Under simulated infection conditions, the extract-loaded hydrogel (P 3 C 2 P) significantly inhibited bacterial growth, attenuated virulence production, and suppressed both motility and biofilm formation. These findings demonstrate a triple-action anti-infective mechanism—mediated by punicalagin, which concurrently targets virulence, motility, and biofilm formation. This work establishes a natural product-based hydrogel platform with significant translational potential for wound management and antimicrobial applications.
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Foods
·
2026
·Vol. 15
·期 5
·829
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IF 6
Jinqiong Xu,Xinyu Yu,Jianbo Liu,Qinglin Sheng
Pseudomonas aeruginosais an important waterborne pathogen that is harmful to food safety and human health. Therefore, the detection ofPseudomonas aeruginosahas become an essential item in food sampling. This article constructs a fluorescent aptamer sensor based on Tb-MOFs, using Tb-MOFs as the fluorescence signal source, colloidal gold (AuNPs) as the signal conversion switch, and aptamers as the target recognition element, to establish a quantitative detection method forPseudomonas aeruginosa. The constructed sensor exhibits a good linear relationship within the concentration range ofPseudomonas aeruginosafrom 1 to 106CFU/mL, with a detection limit of 0.63 CFU/mL. Moreover, the sensor was also applied to the detection ofPseudomonas aeruginosain actual samples of bottled drinking water and orange juice. The fluorescent aptasensor based on Tb-MOFs provides a rapid and sensitive new sensing method for the detection ofPseudomonas aeruginosain food.
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ENVIRONMENTAL POLLUTION
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2025
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IF 7.3
Anhang Li,Zeyu Wang,Changjie Shi,Nan Li,Mengwei Bai,Jiachao Yao,Dzmitry Hrynsphan,Haifeng Qian,Sihan Hu,Jun Wei,Jun Chen
Nitrogen pollution constitutes a critical environmental challenge for aquatic ecosystems, where biological denitrification serves as a critical mechanism for nitrogen removal in wastewater treatment plants (WWTPs). Pseudomonas guariconensis , a key denitrifying bacterium in WWTPs, demonstrates stable symbiotic relationships with organic carbon substrates under operational conditions. This study investigated the biochar-mediated enhancement mechanism of denitrification performance using Pseudomonas guariconensis strain XYH-2. Experimental results revealed that 0.5 % (w/w) biochar addition significantly improved denitrification efficiency, elevating NO 3 − -N removal from 39 % to 94 % within 16 h while reducing N 2 O accumulation by 81.7 % (from 837 to 153 ppm). Electrochemical characterization and extracellular polymeric substances (EPS) analysis demonstrated biochar's role as an electron shuttle, evidenced by a 48.5 % increase in electron transfer system activity (ETSA) and enhanced redox currents. Notably, biochar stimulation elevated the activities of four key denitrifying enzymes: nitrate reductase (NAR, +75.6 %), nitrite reductase (NIR, +25.4 %), nitric oxide reductase (NOR, +33.3 %), and nitrous oxide reductase (N 2 OR, +159.4 %). Structural characterization revealed biochar-induced conformational modifications in N 2 OR that enhanced substrate binding affinity, particularly explaining the dramatic N 2 O mitigation. These findings provided mechanistic insights into biochar-bacteria synergism, proposing an effective strategy for optimizing denitrification processes in nitrogen-contaminated wastewater treatment systems.
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Food Bioscience
·
2026
·
IF 6.2
Mengmeng Ji,Jinwei Li,Liuping Fan,Liang Wang,Shengquan Huang
Pseudomonas aeruginosa , a significant opportunistic pathogen, poses a serious threat to food safety due to its antibiotic resistance and strong biofilm-forming ability. In this study, quercetagetin (Que) was identified as a novel quorum sensing (QS) inhibitor. Reporter strain assays, molecular docking, and dynamic simulation indicated that Que acts as an antagonist of the QS receptors. Que attenuated QS-regulated virulence phenotypes in a dose-dependent manner but showed insufficient antibiofilm activity ( p >0.05) at sub-minimum inhibitory concentration (sub-MIC) levels. In contrast, the combination of 0.5 mmol/L Que with 20 mmol/L D-leucine or D-tryptophan exhibited additive effects on planktonic cells, reducing its survival by 2.19–3.97 log compared to the control. For biofilm cells, the combined treatment potentiated the reduction in cell density by 0.48–1.42 log. Notably, these effects were strain-dependent but were equal to or outperformed those of the ε-polylysine (ε-PL) control. Mechanistically, this enhancement was driven by the disruption of biofilm matrix and cell membrane integrity. This was evidenced by the greater suppression of Acyl-homoserine lactones (AHLs) synthesis and virulence phenotypes, alongside substantial reductions in biofilm biomass and roughness and increased in membrane permeability compared to Que. Furthermore, the combination resulted in repression of key enzymes activities (Na + -K + -ATPase and succinate dehydrogenase) by 29%–38%. These changes indicated a promising strategy to control P. aeruginosa and enhance food safety.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 13.2
Lijin Shi,Chenglong Xue,Xiaoyu Zhao,Yuqian Wang,Qi Shuai
To address the dual challenges of antibiotic resistance and physical barrier in Pseudomonas aeruginosa ( PA ) biofilm infection, a near-infrared light responsive nitric oxide (NO) releasing asymmetric nanomotor (MAGN) was developed to achieve efficient anti-infection through the triple strategy of “targeted recognition - autonomous penetration- synergistic antibacterial”. The MAGN surface modification of galactose enables it to actively target bacteria, thereby enhancing its accumulation at the infection site. Under the irradiation of 808 nm near-infrared light, MAGN generates heat, which on the one hand promotes the dispersion of biofilms, and on the other hand triggers the loaded nitrosoglutathione (GSNO) molecules to release NO gas. The released NO gas not only drives the autonomous movement by activating the asymmetric propulsion mechanism of MAGN to further enhance its permeability but also works in synergy with the photothermal effect to exert antibacterial efficacy deep in the infected site. In a mouse model infected with PA , MAGN demonstrated a remarkable biofilm-eliminating capability in the absence of antibiotics. In conclusion, MAGN establishes a pioneering platform that leverages targeted multi-modal synergy to achieve highly effective, antibiotic-free eradication of recalcitrant PA biofilm infections.
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Biomaterials Science
·
2026
·
IF 5.7
Meilin Qiao,Licheng Gao,Ruibing Yu,Jian-Wei Chen,Xuanrong Sun,Yue Cai
The emergence of multidrug-resistant Pseudomonas aeruginosa (P. aeruginosa) infections, particularly those involving biofilm formation, poses a critical challenge to global healthcare. While polymyxin B (PMB) remains a last-line antibiotic against such infections, its efficacy is severely limited by poor biofilm penetration and adaptive resistance mechanisms. In this study, we developed a biomimetic nanoplatform (PMφ-PLGA-NOR) comprising a poly(lactic-co-glycolic acid) (PLGA) core loaded with norharmane (NOR), coated with PMB-modified P. aeruginosa cytoplasmic membrane (PMφ). This design utilizes the homologous targeting of bacterial membrane components and PMB's affinity for gram-negative pathogens to achieve efficient biofilm penetration. NOR, a βcarboline compound, has been shown to disrupt quorum sensing (QS) by inhibiting PqsA, resulting in significant reduction in biofilm formation. In vitro investigations have demonstrated that PMφ-PLGA-NOR disrupts the integrity of biofilms, thereby enhancing the bactericidal efficacy of PMB by facilitating its deep penetration into the biofilm matrix. In vivo, intraperitoneal administration of Mφ-PLGA-NOR combined with PMB achieved a 2-log reduction in bacterial lung burden. This study underscores the promise of bacterial plasma membrane-based pathogen-mimetic nanoplatforms in potentiating the efficacy of antibiotic adjuvants in conjunction with biofilmpenetrating strategies.
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BIOSENSORS & BIOELECTRONICS
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2020
·
IF 10.257
Wang Shuai,Yu Wei,Liu Zhiyong,Cai Xiaoyin,Zeng Sheng,Zhang Jingran,Gao Yamin,Tan Yaoju,Cao Zhizhong,Guo Lingmin,Hameed H M Adnan,Khan Shahzad Akbar,Khan Zafran,Tian Xirong,Fang Cuiting,Islam Md Mahmudul,Sapkota Sanjeep,Guan Ping,Hu Jinxing,Liu Jianxiong,Zhong Nanshan,Zhang Tianyu
Pseudomonas aeruginosa is an increasingly prevalent pathogen that has become a serious health concern due to an increasing incidence of multidrug-resistant (MDR) hospital-acquired infections. The emergence of MDR- P. aeruginosa coupled with shrinking antibiotic pipelines has increased the demand for new antimicrobials and therapeutics. An effective tool for drug screening both in vitro and in vivo can facilitate the discovery of drugs and regimens for treating P. aeruginosa infection. Here, for the first time, we combined the mini-Tn7 system and Xer/ dif recombinase system to construct a stable and s electable marker- f ree a uto l uminescent P . a eruginosa (SfAlPa) by one step. Afterwards, in vitro and in vivo activities of several antibiotics including amikacin , biapenem , levofloxacin and polymyxin B were assessed using SfAlPa. This study demonstrated that the use of SfAlPa could significantly facilitate rapid real-time evaluating the activities of compounds. Compared to prevailing methods, this method reduces the time, effort, animals and costs consumed in the discovery of new drugs against P. aeruginosa . Additionally, the methodology described in this study could be easily modified for construction of selectable marker-free reporter strain in other Gram-negative bacteria.
研究领域:
微生物学
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感染性疾病
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分子影像学
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抗菌药物研发
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转化医学
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药效学
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细菌遗传工程
·
动物模型
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
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2023
·
IF 6.1
Wanqing Zhang,Haixian Liu,Yuxuan Xiao,Jie Ying,Hongwei Liu
Oxygen evolution reaction (OER) has gained increasing attention among researchers due to its significance in energy conversion. However, the sluggish kinetics of the OER present notable challenges. In this work, we present an energy-efficient, cost-effective, widely applicable, and scaled-up biological preparation method to prepare OER catalytic materials with high activity and stability based on bacteria and inexpensive Ni-Fe foam under mild conditions. Anaerobic sulfate-reducing bacterium Desulfovibrio caledoniensis and aerobic Pseudomonas stutzeri are the typical microorganisms and the OER performance of their products is not clear. Electrochemical measurements show that the catalyst prepared via D. caledoniensis exhibits a low overpotential of only 247 mV to achieve 10 mA cm −2 and a low Tafel slope of 46 mV dec -1 , while the catalyst prepared via P. stutzeri has an overpotential and Tafel slope of 259 mV and 71 mV dec -1 , respectively. Therefore, these catalysts exhibit excellent catalytic activities. Furthermore, they have a good stability of more than 40 h at 10 mA cm −2 which is far superior to that of the majority of reported NiFe-based OER catalysts. This work shows new insights into the preparation and development of high-performance OER catalysts in mild conditions.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Mengyang Xing,Yong Chen,Boqiang Li,Shiping Tian
Patulin is a toxic secondary metabolite produced by several moulds, which contaminates fruits and their products posing serious threats to human health. Though several microorganisms and enzymes have been reported to effectively degrade patulin, separation of them from fruit juice challenges the commercial applications. Here, a Pseudomonas aeruginosa strain TF-06 was isolated, its patulin degradation mechanism and optimum conditions for enzyme immobilization were investigated. The results indicated that TF-06 could degrade patulin into non-cytotoxic E/Z-ascladiol mainly by the activity of intracellular enzymes. For easy separation of enzymes, calcium alginate was selected for immobilization of intracellular enzymes from TF-06. The immobilized enzyme beads were effective in detoxification of patulin in apple juice. The mitigation rate was reached 95%, while there was no negative effect on juice quality. The study provides a promising way to resolve the issue of enzyme separation during mycotoxin biological detoxification in fruit juice.
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MICROBIAL PATHOGENESIS
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2023
·
IF 3.8
Navni Rohatgi,Dhanraj Ganapathy,Palanivel Sathishkumar
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that form biofilms in chronic wounds and is difficult to treat with standard treatment methods. In the present study, flavonoid quercetin-mediated CuONPs (Que-CuONPs) were successfully synthesized and incorporated in the electrospun polycaprolactone (Que-CuONPs-PCL) nanofibrous membrane to eradicate the burn wound infection causing P. aeruginosa biofilm. The fabricated scaffold Que-CuONPs-PCL was characterized using HR-SEM, EDX, XRD, and FTIR. The synthesized Que-CuONPs appeared as spherical in shape with the average size of 36 nm. The crystallite size of the synthesized CuONPs was calculated as 23 nm. Antibacterial activity results shows that the ZOI and MIC of Que-CuONPs against P. aeruginosa was found to be 20 mm and 5 μg/mL, respectively. Antibiofilm assay results indicate the pre-formed P. aeruginosa biofilm was completely eradicated by Que-CuONPs at 8-MIC. The Que-CuONPs-PCL nanofibrous scaffolds exhibits less cytotoxic effects on mouse fibroblast (L929) cells. Finally, this study highlights the fabricated Que-CuONPs-PCL nanofibrous scaffolds exhibits an excellent antibiofilm effect against P. aeruginosa biofilm with a great biocompatibility .
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