Journal of Environmental Sciences
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2022
·
IF 6.796
Yunhao Zhang,Yi Qian,Ming Zhang,Weichuan Qiao
The wastewater discharge from the process of chrome plating, which contains 6:2 chlorinated polyfluorinated ether sulfonate (F-53B) and chromium (Cr), may be toxic to biofilm. In this study we found that the biofilm formed by Pseudomonas aeruginosa PAO1 was inhibited by exposure to a combination of F-53B and Cr(VI). The combined pollution damaged the cell membranes and the structure of the biofilm, and inhibited the production of the Pseudomonas quinolone-based signal, which affected biofilm formation. Moreover, the secretion of extracellular polymeric substances decreased as a result of this combined exposure. Exposure to F-53B and Cr(VI) individually or in combination could induce the excessive accumulation of intracellular reactive oxygen species (ROS), and the ROS positive rate of the bacteria increased under the treatment with 0.2 mmol/L of Cr(VI) and 250 nmol/L of F-53B, respectively. In addition, the activities of superoxide dismutase (SOD) and catalase (CAT) were enhanced for scavenging ROS in the bacteria that were exposed to Cr(VI) and F-53B. As an antioxidant, rutin was used to repair the toxicity of Cr(VI) and F-53B towards the biofilm formed by the bacteria. When rutin was added to the bacteria medium, with either Cr(VI) or F-53B as pollutant, or with the combined pollutants, the extracellular protein content of the bacteria recovered to 0.84, 0.94, and 0.85 times that of the control, respectively. Meanwhile, the accumulation of ROS and the activities of SOD and CAT decreased, which indicated that the addition of rutin can alleviate the oxidative stress and promote the antioxidant stress system.
研究领域:
环境科学
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微生物学
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毒理学
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PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 3.9
Jinyue Chai,Xue Chen,Chengcheng Jin,Fang Chai,Miaomiao Tian
In this study, AgCl was used as the carrier matrix, TiO 2 was introduced on the substrate surface, carboxyphenylboronic acid (CPBA) was used as the boronate affinity functional monomer , and Rutin (RT) as template to prepare molecular imprinted adsorbent AgCl@TiO 2 @B-MIPs (B-MIPs). The effects of synthesis conditions, solid-phase extraction (SPE) conditions, selectivity, competitiveness, reproducibility, and reusability were evaluated in detail. Under the optimum adsorption conditions, the maximum adsorption capacity is 31.70 mg g −1 . The utility of B-MIPs as a SPE adsorbent for RT was supported by an abundance of pore structure, as well as boric acid affinity sites that facilitate rapid binding of adsorbents to templates. The linear range is 0.05–50 mg L −1 , and the limit of detection (LOD) is 0.023 mg L −1 . The method was used for the determination of RT in sunscreen and isolation cream by high-performance liquid chromatography (HPLC). The target analytic recoveries are in the range of 84.7–98.0% (relative standard deviation is less than 2.4%, n = 3). Furthermore, this work provides a potential possibility to detect other cis -diol containing analytes in the complex matrix.
研究领域:
分析化学
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药学
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材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Qing Huang,Chen-dong Ling,Wen-feng Zhuge,Wei Huang,Feng-ping Liu,Cui-zhong Zhang,Jin-yun Peng,Fu-xing Huang
An efficient electrochemical sensor for rutin determination based on CeO 2 /NiCo 2 S 4 acanthosphere-like microspheres and multi-walled carbon nanotubes (MWCNTs) modified electrode was proposed. In this work, we design an acanthosphere-like CeO 2 /NiCo 2 S 4 heterojunction by an “in situ growth” method of hydrothermal process. Then MWCNTs were introduced into heterostructured microspheres to form composites of CeO 2 /NiCo 2 S 4 /MWCNTs. The morphology and elemental properties of the electrochemical materials were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The redox mechanism of rutin on the working electrode surface and the electrochemical properties were investigated by cyclic voltammetry (CV), chrono coulometry (CC) and electrochemical impedance spectroscopy (EIS). The results show that the CeO 2 /NiCo 2 S 4 /MWCNTs modified glassy carbon electrode exhibited excellent electric conductivity and electrocatalytic activity toward rutin, and increased effective working area. Under the optimized condition, the fabricated sensor expressed a good linear response for rutin analysis ranging from 0.5 to 65.4 μM with a low detectable limit of 4.37 × 10 -2 μM (S/N = 3). The electrochemical sensor shows good reproducibility and reliable stability, high sensitivity and selectivity, and has been successfully applied for rutin determination in orange juice and human urine samples.
研究领域:
分析化学
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材料科学
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电化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Weisong Wu,Limei Liu,Yingwei Zhu,Jingbin Ni,Jian Lu,Xiaoli Wang,Li Ma,Yanjun Jiang
In patients suffering from inflammatory bowel diseases (IBDs), the immune system is disrupted and the intestinal barrier function is compromised. Here, six zinc-flavonoid particles were produced by one-step reaction via changing flavonoids (myricetin, quercetin, and rutin) and solvent (water and ethanol), and then their cytocompatibility and ability to scavenge H2O2, free radicals, and LPS-induced ROS were compared. Zinc-rutin particles (W-ZnRT) composed of rutin (78.92 wt %), Na12[ZnPO4]12·12H2O (6.76 wt %), and crystal water were screened out because W-ZnRT exhibited 80.8 ± 15% cell viability against RAW264.7, could rapidly scavenge 78.1 ± 1% of H2O2 and 71.6 ± 2% of DPPH within 30 min, and reduced LPS-increased intracellular ROS to normal levels. In addition, the therapeutic effects of rutin and W-ZnRT were also compared in dextran sulfate sodium (DSS)-induced acute and chronic colitis in mice. W-ZnRT was superior to rutin alone in chronic colitis (n = 9), although they were equally effective in acute colitis (n = 7). Compared to rutin, 11 oral doses of W-ZnRT (40 mg kg–1) significantly improved intestinal permeability (p = 0.0299) and colon length (p = 0.0025), reduced intestinal proinflammatory factors (IL-6, IL-1β, and TNF-α), and upregulated tight junction proteins to maintain intestinal barrier function. Taken together, these results identified W-ZnRT as an efficient and safe therapeutic strategy for IBD.
研究领域:
药理学
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免疫学
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胃肠病学
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分子生物学
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DOI
PubMed
高影响因子
Pharmaceutics
·
2026
·Vol. 18
·期 7
·872
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IF 6.9
Weinan Li,Sisi Yan,Yingying Gao,Yuhan Fu,Yutong Mei,Yanhong Wang,Zhixin Yang
Background: The highly heterogeneous and dynamically evolving tumor microenvironment leads to the development of drug resistance and recurrence in traditional therapies. Although immunotherapy demonstrates unique advantages, its clinical utility remains constrained by the suboptimal immunogenicity and the limited effect of monotherapy. Herein, a hybrid drug delivery system based on a spatiotemporal hierarchical controlled-release strategy was proposed to achieve dual immunotherapy with immune checkpoint blockade (ICB) and immunogenic cell death (ICD) to promote synergistic anti-tumor therapy.Methods: A liposome–micelle hybrid drug delivery system (RUT-RPP-LP) was constructed using a lipid bilayer composed of dioleoyl phosphatidylethanolamine/hemisuccinyl cholesterol to encapsulate rutin (RUT) and to form an inner cavity-encapsulated resveratrol micelle (RPP). RUT-RPP-LP was characterized, and its pH sensitivity and release behavior were investigated. Subsequently, a colon cancer tumor-bearing mouse model was constructed to evaluate the in vivo targeted anti-tumor effect and biological safety. On this basis, the combined mechanism of ICB and ICD was preliminarily explored.Results: RUT-RPP-LP possessed excellent formulation characteristics, stability, and biocompatibility, achieving graded controlled release of drugs via responding to the TME and lysosomal acidity, respectively. Obviously, RUT-RPP-LP could specifically target the tumor site, induce the occurrence of ICD, and simultaneously block the PD-1/PD-L1 immune checkpoint signaling pathway, thereby enhancing the function of T cells and inducing apoptosis of tumor cells.Conclusions: The RUT-RPP-LP based on the hierarchical controlled-release strategy exerted a spatiotemporally coordinated enhancement of anti-tumor immunity, and may provide a novel combinatorial approach to overcome the low response of immunotherapy in solid tumors.
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.7
Yupei Sun,Yanhui Yi,Yaxi Xu,Xiangyang Zhang,Xiaofeng Mou,Feng Zhao,Jianzeng Xin,Sheng Liu
Meat color directly affects consumers’ purchase intention of meat, which is mainly affected by myoglobin (Mb). The extract of Flos sophorae immaturus (FSI) and its main active ingredient rutin (52% of the FSI extract) show potential in preserving beef color. In this study, their protective effects and mechanisms were investigated by spectral analysis, molecular docking, and molecular dynamics simulation. Results indicated that rutin exhibited superior color protection: Beef treated with rutin maintained higher redness and brightness during 7 days of 4 °C storage, with MetMb% maintained at 45–47% and stable pH values around 5.5. Mechanistically, rutin spontaneously formed stable noncovalent complexes with Mb mainly through van der Waals forces and hydrogen bonds, inducing Mb aggregation, altering its secondary structure, reducing surface hydrophobicity and solubility, and enhancing thermal stability (denaturation temperature increased from 77.80 to 133.25 °C). These findings provide a theoretical foundation for developing novel natural meat color protectants.
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DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
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2019
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IF 4.44
Satar Tursynbolat,Yrysgul Bakytkarim,Jianzhi Huang,Lishi Wang
The simultaneous electrochemical determination of myricetin and rutin remains a challenge due to their indistinguishable potentials. To solve this problem, we constructed a ternary platinum nanoparticle, reduced graphene oxide, multi-walled carbon nanotubes (Pt@r-GO@MWCNTs) nanocomposite via a facile one-pot synthetic method. Under the optimized conditions, the ternary Pt@r-GO@MWCNTs nanocomposite exhibited good electrocatalytic activity toward myricetin and rutin via solid phase extraction and excellent performance for the simultaneous determination of myricetin and rutin. The oxidation peak current of myricetin was proportional to its concentrations in the range of 0.05–50 μM with a detection limit of 0.01 μM (S/N = 3). The linear range for rutin was 0.05–50 μM with a detection limit of 0.005 μM (S/N = 3). The ternary nanocomposite sensor also exhibited good reproducibility and stability, and was successfully used for the simultaneous determination of myricetin and rutin in real orange juice samples with recoveries ranging between 100.57% and 108.46%.
研究领域:
分析化学
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材料科学
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生物医学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 22
·7761
·
IF 4.927
Yuhong Li,Jianxiong Tang,Yueli Lin,Jiejun Li,Yaqi Yang,Pengcheng Zhao,Junjie Fei,Yixi Xie
Rutin, a natural flavonol glycoside, is widely present in plants and foods, such as black tea and wheat tea. The antioxidant and anti-inflammatory effects of flavonoids are well known. In this study, a new electrochemical rutin sensor was developed using multiwalled carbon nanotubes/aluminum-based metal–organic frameworks (MWCNT/CAU-1) (CAU−1, a type of Al-MOF) as the electrode modification material. The suspension of multiwalled carbon tubes was dropped on the surface of the GCE electrode to make MWCNT/GCEs, and CAU−1 was then attached to the electrode surface by electrodeposition. MWCNTs and CAU−1 were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Due to the synergistic effect of CAU−1 and MWCNT-COOH, the prepared sensor showed an ultrasensitive electrochemical response to rutin. Under optimized conditions, the sensor showed a linear relationship between 1.0 × 10−9~3.0 × 10−6M with a detection limit of 6.7 × 10−10M (S/N = 3). The sensor also showed satisfactory stability and accuracy in the detection of real samples.Keywords:electroanalysis;pyrolysis;carbon materials;drug analysis;modified electrode
研究领域:
分析化学
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材料科学
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电化学
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DOI
PubMed
高影响因子
MOLECULES
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2022
·Vol. 27
·期 22
·8021
·
IF 4.927
Tao Zhang,Qingxue Ji,Jiayi Song,Haiming Li,Xing Wang,Haiqiang Shi,Meihong Niu,Tingting Chu,Fengshan Zhang,Yanzhu Guo
The poor water solubility, large particle size, and low accessibility of cellulose, the most abundant bioresource, have restricted its generalization to carbon dots (CDs). Herein, nitrogen and sulfur co-doped fluorescent carbon dots (N, S-CDs) were hydrothermally synthesized using cellulose nanocrystals (CNC) as a carbon precursor, exhibiting a small particle size and excellent aqueous dispersion. Thiourea was selected as a nitrogen and sulfur dopant to introduce abundant fluorescent functional groups into N, S-CDs. The resulting N, S-CDs exhibited nanoscale size (6.2 nm), abundant functional groups, bright blue fluorescence, high quantum yield (QY = 27.4%), and high overall yield (16.2%). The excellent optical properties of N, S-CDs endowed it to potentially display a highly sensitive fluorescence “turn off” response to rutin. The fluorescence response for rutin allowed a wide linear range of 0–40 mg·L−1, with a limit of detection (LOD) of 0.02 μM, which revealed the potential of N, S-CDs as a rapid and simple sensing platform for rutin detection. In addition, the sustainable and large-scale production of the N, S-CDs in this study paves the way for the successful high-value utilization of cellulose.Keywords:cellulose nanocrystals;carbon dots;hydrothermal method;fluorescent sensing
研究领域:
材料科学
·
化学
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DOI
PubMed
高影响因子
APPLIED CLAY SCIENCE
·
2023
·
IF 5.6
Pierre Dramou,Yiyang Sun,Xu Ni,Fangqi Wang,Hua He
In this study, a three-dimensional nanocomposite with magnetic features made of magnetic iron oxide nanoparticles (Fe 3 O 4 ), graphene oxide (GO) and halloysite nanotube (Hal) was prepared and introduced for rutin separation from real sample based on magnetic solid phase extraction (MSPE) process. Hal was modified with chitosan (Hal-COS) and showed positive charge, which can combine with GO by electrostatic force self-assembly to fabricate Hal-GO. Based on this, in-situ growth of magnetic sphere on the surface of Hal-GO demonstrated high specific surface area, chemical and thermal stability, water solubility, and capacity of easy separation in aqueous solution. Several characterizations including FT-IR, HRTEM , TGA and XRD evidenced the successful synthesis of the nanocomposite. The results showed that the optimum adsorption pH was 6 and the optimum adsorption temperature was 25 °C. In addition, further experiments showed that the adsorption process conformed to the Langmuir adsorption model and pseudo-second-order kinetic model , which indicated that the maximum equilibrium adsorption capacity was 107.8 mg g −1 and the maximum equilibrium adsorption time was 120 min. With the optimized conditions, the methodological validation experiments showed that the linear range of MSPE based on Fe 3 O 4 @(Hal-GO) combined with HPLC was 10–100 μg mL −1 , the correlation coefficient of the fitting equation was 0.9991, the LOD and LOQ reached 0.87 μg mL −1 and 2.9 μg mL −1 respectively. It was confirmed that prepared Fe 3 O 4 @(Hal-GO) supported efficient enrichment of rutin when the results were compared to those of other studies.
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高影响因子
FOOD CONTROL
·
2023
·
IF 6
Jie Tang,Lincai Peng,Ahmad Ali,Siyu Zhao,Ziyue Zeng,Ke Yuan,Shun Yao
As the famous naturally occurring flavonoid , rutin exists in many foods , beverages, and their processed functional products. To realize highly efficient analysis for it, a novel supramolecular hydrogel was developed as the electrode modification material for sensor. Here 12 amino acid-based ionic liquids ([C n mim][AA]) were synthesized and 6 of them could self-aggregate to form hydrogels. Especially, all the hydrogels possessed dual sensitivities to temperature and pH, as well as relatively excellent electrocatalytic performance. A three-layer electrochemical sensor [C n mim][AA]/MWCNTs/CS/GCE was prepared by decorating glass carbon electrode (GCE) with hydrogels, multiwalled carbon nanotubes (MWCNTs) and chitosan (CS) to detect rutin. After comprehensive characterization, computation and systematic investigation, the results indicated that Ipa was linearly related to the concentration of rutin in the range of 0.5–5 μM and 5–100 μM with a limit of detection (LOD) of 0.05 μM (S/N = 3). The selectivity, repeatability, stability and potential in electrochemical analysis of the developed supramolecular sensor were proved after analysis of black tartary buckwheat tea and related health-care pills.
研究领域:
分析化学
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食品科学
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生物医学工程
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DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Yilin Wang,Jia Chen,Chenxi Wang,Li Zhang,Yaqi Yang,Chao Chen,Yixi Xie,Pengcheng Zhao,Junjie Fei
As a common antioxidant and nutritional fortifier in food chemistry, rutin has positive therapeutic effects against novel coronaviruses. Here, Ce-doped poly(3,4-ethylenedioxythiophene) (Ce-PEDOT) nanocomposites derived through cerium-based metal-organic framework (Ce-MOF) as a sacrificial template have been synthesized and successfully applied to electrochemical sensors. Due to the outstanding electrical conductivity of PEDOT and the high catalytic activity of Ce, the nanocomposites were used for the detection of rutin. The Ce-PEDOT/GCE sensor detects rutin over a linear range of 0.02–9 μM with the limit of detection of 14.7 nM (S/N = 3). Satisfactory results were obtained in the determination of rutin in natural food samples (buckwheat tea and orange). Moreover, the redox mechanism and electrochemical reaction sites of rutin were investigated by the CV curves of scan rate and density functional theory. This work is the first to demonstrate the combined PEDOT and Ce-MOF-derived materials as an electrochemical sensor to detect rutin, thus opening a new window for the application of the material in detection.
研究领域:
分析化学
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电化学
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材料科学
·
食品化学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）