Drug Metabolism and Pharmacokinetics
·
2019
·
IF 1.874
Yang Zhang,Chen Su,Jinxiu Lei,Lu Chen,Haihong Hu,Su Zeng,Lushan Yu
Mandelic acid (MA) is generally used as a biomarker of the exposure of styrene , which is classified as a class of hazardous environmental pollutants, and also used as an important chiral intermediate in pharmaceutical industry. The previous studies have found the excretion of phenylglyoxylic acid (PGA) in human and rat, a metabolite of MA, was mainly from S- MA rather than R -MA. The metabolic mechanism, however, is not clear. In order to explore the possible metabolic mechanism, the enzyme types involved in the stereoselectivity metabolism of MA were firstly studied, and then human and rat long-chain 2-hydroxy-acid oxidase 2 (HAO2) were recombinantly expressed to study the metabolic profiles of S- MA and its analogues. The results indicated that HAO2 might catalyze the stereoselectivity metabolism of S- MA in rats. Human HAO2 ( h HAO2) and rat HAO2 ( r HAO2) isozymes β 1 and β 2 were successfully cloned and expressed with high purity and good enzyme activities. The enzyme kinetic profiles of these enzymes were different for S- MA and analogues. The order of catalytic efficiency for h HAO2 and r HAO2, however, was reverse. It might be relevance to the difference in active amino acid residues and loop 4 in human and rat L-2-hydroxy acid oxidase isozyme B crystal structures.
研究领域:
环境科学
·
生物化学
·
毒理学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2022
·
IF 12.92
Yue Huang,Kai Dai,Jinfeng Zhang,Graham Dawson
Non-stoichiometric W 18 O 49 (WO) prepared by solvothermal method has excellent NIR absorption due to the localized surface plasmon resonance effect caused by oxygen vacancies. This has great potential in the field of using sunlight to convert carbon dioxide into organic fuels. In addition, through the amination of CdSe, the one-dimensional/two-dimensional step-scheme (S-scheme) WO/CdSe-diethylenetriamine (WO/CdSe-D) photocatalyst with electron transmission channels driven by visible light to NIR light is constructed by microwave solvothermal method. The LSPR of WO and the synergistic effect of coupling semiconductors to construct S-scheme heterojunctions can improve light utilization and achieve efficient charge carrier transfer efficiency. The optimized photocatalyst of 35%WO/CdSe-D has the best CO 2 reduction performance compared to WO and CdSe-D, and the yield is 25.37 µmol h –1 g –1 . X-ray photoelectron spectroscopy was used to verify the charge transfer path of the S-scheme WO/CdSe-D heterojunction. This work provides a possibility for the application of non-stoichiometric oxides rich in oxygen vacancies in the field of photocatalytic CO 2 reduction.
研究领域:
材料科学
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环境科学
·
能源科学
✓ 已验证
DOI
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2024
·
IF 3.1
Chao-Ping Lin,Chen-Hui Xu,Fan-Yu Meng,Shi-Ping Song,Zhe-Ming Wu,Ren-Chao Zheng,Yu-Guo Zheng
A chemoenzymatic process combing nitrilase-catalyzed hydrolysis and continuous flow racemization was developed for highly efficient manufacturing of (S)-pregabalin. Nitrilase was immobilized on epoxy resin and employed for the biosynthesis of (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the precursor of (S)-pregabalin. Isobutylsuccinonitrile (IBSN) (700 mM) was converted to (S)-CMHA with a conversion of 45.3% and ee of 99.5%. Meanwhile, the unreacted (R)-IBSN was continuously racemized by the γ-alumina-based solid base catalysts (ASBCs) in a packed bed reactor, and an outstanding space-time yield of 6.76 mol L–1 day–1 was achieved. Moreover, the ASBCs exhibited excellent operational stability in the racemization system and retained 85.9% catalytic activity after 20 days of continuous racemization operation. (S)-CMHA was subsequently separated and purified by a membrane separation system in 95% yield and was hydrogenated in one step to (S)-pregabalin in a yield of 88%. These obtained results exhibited the efficiency of the chemoenzymatic process and the great potential for industrialization of (S)-pregabalin.
研究领域:
化学工程
·
生物催化
·
药物化学
✓ 已验证
DOI
高影响因子
Biomaterials Advances
·
2025
·
IF 6
Ying Sun,Yining Cai,Shanshan Xu,Ningning Zhai,Zirui Wang,Zongpu Qiu,Shuying Han,Yuanqing Wei,Rui Liu,Hao Wu
While the regulatory roles of biomaterial topology in bone regeneration are recognized, the specific osteogenic mechanisms driven by distinct morphological features, particularly without biochemical cues, remain poorly understood. This study aimed to independently elucidate the osteogenic potential and underlying mechanisms solely attributable to the distinctive “S”-grooved topology derived from cuttlebone. Inspired by cuttlebone, we successfully fabricated a biomimetic polycaprolactone (PCL) membranes sheet with “S”-grooved topology using liquid silicone mold replication combined with PCL recasting techniques. Comprehensive characterization (SEM, CLSM) confirmed precise replication of the native “S”-grooved morphology (width: 100 ± 30 μm, depth: 20 ± 6 μm). In vitro studies revealed that these grooves promoted directional adhesion and proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) via contact guidance, as evidenced by cytoskeleton alignment. Furthermore, the “S”-grooved topology significantly enhanced osteogenic differentiation of mouse pre-osteoblasts (MC3T3-E1), demonstrated by increased alkaline phosphatase (ALP) activity and mineralized nodule formation. Mechanistic investigations using RT-qPCR, Western blot, and dimethyl labeling-based quantitative proteomics on rBMSCs identified key involvement of the Rap1-ERK signaling pathway. Specifically, the “S”-grooved topology upregulated Rap1 expression and enhanced ERK phosphorylation, leading to increased expression of osteogenic markers (e.g., Runx2, OPN). These findings demonstrate that the biomimetic “S”-grooved topology alone, through contact guidance and activation of the Rap1-ERK mechanotransduction pathway, significantly enhances osteogenic differentiation, providing a foundation for designing topologically optimized biomaterials for bone regeneration biomimetic PCL membranes sheet with the “S”-grooved topology.
研究领域:
生物材料
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组织工程
·
细胞生物学
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生物医学工程
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材料科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2024
·Vol. 20
·期 6
·1041-1048
·
IF 2.9
Li Shizheng,Lv Yanwei,Liu Xiaoying,Yuan Yuan
Chronic wounds in diabetic patients have become a common complication. However, current wound management tends to yield unsatisfactory outcomes and may give rise to complications. Therefore, we prepare a nano-structured lipid carrier by incorporating a 20(S)-Protopanaxadiol (PPD)-loaded
lipid nanoparticles (PPD-N) into a silicone elastomer (PPD-NS) for treating chronic diabetic wounds and assess its therapeutic efficacy in promoting diabetic wound healing and vascular regeneration in a mouse model. Our findings revealed that the prepared PPD-N showed uniform distribution
with spherical morphology and an average diameter of approximately 130 nm. Moreover, PPD-NS exhibited a wound closure rate of 92.1% after 48 hours of treatment and demonstrated enhanced vascular regeneration. Therefore, it can be concluded that the prepared PPD-NS can effectively be used in
diabetic wound repair and vascular regeneration. This study provides a promising potential therapeutic option for diabetic wounds.
研究领域:
药学
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生物医学工程
·
伤口护理
·
糖尿病研究
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Longkai Pan,Li Yao,Hui Mei,Hongxia Liu,Zhipeng Jin,Shixiang Zhou,Minggang Zhang,Gangqiang Zhu,Laifei Cheng,Litong Zhang
In this study, a novel 3D Bi 2 S 3 /P-ZnO S-scheme gyroid metamaterial was prepared by 3D printing and the hydrothermal method. The unique gyroid macrostructure and nanoarray microstructure offer a large volume of active sites for the photothermal catalytic reaction, thus improving the overall utilization rate of light. ZnO obtained by sintering the precursor produces oxygen vacancies, primarily due to P doping. This enables it to have a photothermal effect. After loading Bi 2 S 3 , the photothermal effect of P-ZnO and Bi 2 S 3 is coupled, further increasing the surface temperature of composite structure and speeding up the reaction rate. P-ZnO and Bi 2 S 3 formed a S-scheme heterojunction, promoting the separation and transfer of photogenerated carriers. Under simulated sunlight, the CO and CH 4 yield of Bi 2 S 3 /P-ZnO S-scheme gyroid metamaterials are 8.87 and 1.49 μmol h −1 . These are 3.45 times and 4.65 times of P-ZnO gyroid structure, respectively. Through SEM, TEM, XPS, ESR and in-situ FTIR characterizations, the transfer paths of photogenerated carriers between heterojunctions were revealed, and the reaction paths of photothermal catalytic CO 2 reduction were explored. The co-design of 3D structure and material allows for a novel concept for further improving photothermal catalytic performance.
研究领域:
材料科学
·
化学工程
·
环境科学
·
纳米技术
✓ 已验证
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Yu Zhang,Wangfeng Cai,Yajie Guo,Yan Wang
In this work, a nanosheet-assembled core-shell structure composite of Co-Ni-S@CoNi-LDH was prepared on Ni foam via hydrothermal synthesis , in which Co-Ni-S nanowires as cores were assembled with the shell of CoNi-LDH nanosheets. The combination of the high conductivity of Co-Ni-S, the high specific surface area of CoNi-LDH and the multivalent state of Ni and Co provided more active sites, short and effective ion transport paths, and great synergy, which played significant roles in the excellent performance of Co-Ni-S@CoNi-LDH. Meanwhile, the nanosheet-assembled core-shell structure improved the overall stability. Based on the characterisation and the performance measurements, the Co-Ni-S@CoNi-LDH electrode showed an excellent electrochemical performance. The Co-Ni-S@CoNi-LDH electrode acquired a specific capacitance of 2414 F/g at a 1 A/g current density and a high cycle stability with an 89.7% retention after 3000 cycles. The assembled hybrid supercapacitor Co-Ni-S@CoNi-LDH//AC delivered a high energy density of 59.11 Wh kg −1 at an 855.43 W kg −1 power density, as well as an excellent rate performance of 81% after 5000 cycles at a 5 A/g current density, showing good potential for practical applications.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry A
·
2022
·Vol. 10
·期 42
·22791-22802
·
IF 14.511
Xing-Ye Chen,Muhammad Ishaq,Nafees Ahmad,Rong Tang,Zhuang-Hao Zheng,Ju-Guang Hu,Zheng-Hua Su,Ping Fan,Guang-Xing Liang,Shuo Chen
The existence of various detrimental defects inside the absorber layer is a major obstacle limiting the performance of kesterite Cu2ZnSn(S,Se)4 (CZTSSe) thin-film solar cells. Cationic substitution is a viable strategy for overcoming this challenge and has drawn tremendous research attention. In this work, a dual-cation substituted CZTSSe involving Ag and Ti to separately occupy partial Cu and Sn lattice sites is proposed. Under 6% Ag and 1% Ti dual-cation substitution, the champion CZTSSe device delivers a highly interesting efficiency of 12.73%, accompanied by an outstanding VOC of 530 mV. Further investigation demonstrates that Ag introduction can improve the density and crystallinity of CZTSSe thin films while decreasing the concentration of CuZn acceptor defects. Further Ti substitution can effectively eliminate fine grains and reduce the defect density of CuSn and [2CuZn + SnZn] defects, as well as the core level defects. This work explores the synergistic effect of Ag, Ti dual-cation substitution in CZTSSe solar cells for the first time, and the clarified device performance improvement mechanism opens up promising possibilities for the bright development of CZTSSe thin-film photovoltaic technology.
研究领域:
材料科学
·
光伏技术
·
半导体物理
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Worku Lakew Kebede,Dong-Hau Kuo,Fekadu Tadesse Bekena,Lalisa Wakjira Duresa
Environmentally toxic organic pollutants, namely methylene blue (MB), neutral red (NR), Rhodamine B (RhB), and methyl orange (MO) dyes contain highly toxic, carcinogenic, non-biodegradable, and colored pigments which cause harm for humans and aquatic organisms even at low concentrations. To detoxify these toxic organic pollutants from the wastewater, the bimetallic solid solution-typed In–Mo(O,S) 2 catalyst with various indium (In) contents were synthesized at low temperature through a simple precipitation method. The morphological, structural, chemical compositions, electrochemical and optical properties of the catalysts were thoroughly characterized. The photodegradation performance of the In–Mo(O,S) 2 catalysts over the cationic, anionic and neutral dyes were studied under visible light irradiation. It has been observed that the photocatalytic activity was enhanced as In was added to the Mo(O,S) 2 catalyst, and In–Mo(O,S) 2 -20 was found to be the best composition to completely degrade four organic dyes. The dye degradation had rate constant values of 9.5 × 10 −2 min −1 , 6.3 × 10 −2 min −1 , 4.4 × 10 −2 min −1 , and 15.7 × 10 −1 min −1 for MB (20 ppm), NR (20 ppm), RhB (10 ppm), and MO (10 ppm) dyes, respectively. The active species for degradation of MB is different from those for RhB and MO. Single phase In–Mo(O,S) 2 -20 capable to degrade four kinds of dyes at a fast rate is a good photocatalyst.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Dongxia Chen,Jingjin Zhao,Liangliang Zhang,Rongjun Liu,Yong Huang,Chuanqing Lan,Shulin Zhao
Calcium ion (Ca2+) plays crucial roles in the signal transduction pathways associated with various physiological and pathological events. Monitoring intracellular Ca2+ is of great significance for cell biology research. Here, we report the use of biomass quantum dots (BQDs) as a fluorescent reporter for imaging of intracellular Ca2+, based on the inner-filter-mediated luminescence which was assisted by a Ca2+ chelator alizarin red S (ARS). BQDs were prepared by hydrothermal heating of capsicum. The absorption of ARS overlaps with the excitation of the BQDs, the fluorescence of BQDs being quenched through the inner-filter effect. But the absorption of Ca-ARS complex red shifts and shows a poor inner-filter effect. Thus, Ca2+ can be detected by the inner-filter-mediated luminescence using the BQDs-ARS nanohybrid system. Using the proposed nanosystem, the imaging of intracellular Ca2+ and real-time monitoring of the Ca2+ level change under histamine stimulation were also achieved. Thus, this nanosystem holds potential applications in other Ca2+-related signal transduction study.
研究领域:
细胞生物学
·
纳米技术
·
生物医学工程
·
荧光成像
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Jiaxin Li,Chongchao Yao,Bo Song,Zhihao Zhang,Andreas Libonati Brock,Stefan Trapp,Jing Zhang
Microbial fuel cells (MFCs) have great promise for power generation by oxidizing organic wastewater, yet the challenge to realize high efficiency in simultaneous energy production and resource recovery remains. In this study, we designed a novel MFC anode by synthesizing S-doped NiFe 2 O 4 nanosheet arrays on carbon cloth (S10-NiFe 2 O 4 @CC) to build a three-dimensional (3D) hierarchically porous structure, with the aim to regulate the microbial community of sulfur-cycling microbes in order to enhance power production and elemental sulfur (S 0 ) recovery. The S10-NiFe 2 O 4 @CC anode obtained a faster start-up time of 2 d and the highest power density of 4.5 W/m 2 in acetate-fed and mixed bacteria-based MFCs. More importantly, sulfide removal efficiency (98.3 %) (initial concentration of 50 mg/L S 2− ) could be achieved within 3 d and sulfur (S 8 ) could be produced. Microbial community analysis revealed that the S10-NiFe 2 O 4 @CC anode markedly enriched sulfur-oxidizing bacteria (SOB) and promoted enrichment of SOB and sulfate-reducing bacteria (SRB) in the bulk solution as well, leading to the enhancement of power generation and S 0 recovery. This study shows how carefully designing and optimizing the composition and structure of the anode can lead to the enrichment of a multifunctional microbiota with excellent potential for sulfide removal and resource recovery.
研究领域:
环境工程
·
微生物学
·
能源技术
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2016
·Vol. 133
·期 17
·
IF 1.866
Ruiqing Hu,Yuping Pi,Na Wang,Qian Zhang,Jinqi Feng,Weilin Xu,Xia Dong,Dujin Wang,Hongjun Yang
Biodegradable polymers have received increased attention due to their potential application in the medicine and food industries; in particular, poly(lactic acid) (PLA) is a primary biopolymer because of its excellent biocompatibility and absorbability features. In this study, a porous PLA membrane was fabricated by phase separation using water microdroplets produced by an ultrasonic atomizer as a coagulation bath. The formation of S-type clusters was attributed to the slow exchange rate between solvent and coagulant, which provided time for the movement of polymer molecules. The effect of preparation conditions on the structure of S-type clusters, including polymer concentration in solution and ambient temperature was investigated. The PLA porous membrane prepared from water micro-droplets with different morphology compared with the sample fabricated from distilled water has potential application in fields of tissue engineering and artificial organ generation. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43355.
研究领域:
生物材料学
·
高分子科学
·
组织工程
·
再生医学
·
纳米结构制备
·
相变动力学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）