SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ling-Xin Kong,Yan-Xin Zhang,Wei-Gang Zhang,Yun-Shan Zhang,Tie-Yi Yan,Peng-Cheng Geng,Biao Wang
Lab-on-tip technology opens a new door for optical fiber surface plasmon resonance (SPR) sensors. In this paper, we first demonstrate an ultra-compact reflective SPR microtip probe on the flat fiber end in experiment, which has a length of tens of microns and a diameter of several microns. Simulations and experiments show that this new fiber SPR microtip probe coated with a nano-goldfilm has excellent refractive index (RI) sensing characteristic and great potential for biochemical detection at single cell level. Based on the high RI sensitivity, a bio-probe assembled with Chitosan/Polysodium Styrene Sulfonate (Cs/PSS) nano-polyelectrolyte film and the sheep anti-human IgG-HRP is prepared to recognize human IgG specifically. Due to the high surface sensitivity, the SPR microtip probe exhibits an ultra-low detection limit for human IgG. Due to the high bulk sensitivity and high thermo-optical coefficient (TOC) of polydimethylsiloxane (PDMS), a reference temperature probe based on SPR microtip is proposed, which is used to compensate for the temperature cross-sensitivity of the bio-probe. This new SPR microtip probe can be used not only in optical fiber sensor, but also in Tip-Enhanced Near-Field Raman Microscopy and Scanning Near-Field Optical Microscope.
研究领域:
光纤传感器
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表面等离子体共振(SPR)技术
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生化传感
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纳米技术
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显微镜
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DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Xiuling Xue,Xiaoyi Chen,Zongyu Zhang,Guofeng Fan,Tingting Ma
Graphite carbon nitride (g-C 3 N 4 ) has a strong photocatalytic capability to degrade ionic organic compounds due to its unique electrical structure with numerous marginal amino groups. A photocatalyst was fabricated via a facile in-situ sedimentation–calcine–hydrothermal method by anchoring AgBr and Bi 2 WO 6 onto the framework of phosphorus-doped g-C 3 N 4 (PCAB). The photocatalyst has been proven to have good photocatalytic degradation effect on different ionic organic pollutants. The removal rate of tetracycline, the target pollutant, can reach 99.2%, which was 1.83 times higher than that of g-C 3 N 4 within 60 min under visible light. Moreover, the degradation rates of dyes containing azo bonds and -SO 3 H can reach 100% within 20 min, as determined by the electrical relationship between the catalyst and the dye. The excellent performance can be attributed to the synergistic effect of the formation of mid-gap in g-C 3 N 4 after phosphorus doping, improvements in charge carrier transfer and the low recombination rate based on the construction of dual Z-scheme heterojunction and Ag bridge. In addition, cycling experiments and characterizations illustrated the high stability and reusability of PCAB. The main active species during photocatalytic process was·O 2 − , whereas h + and·OH played a minimal role. Ultimately, a dual Z-scheme mechanism was proposed to explain the photocatalytic reaction.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
LANGMUIR
·
2013
·
IF 4.187
Tao Wang,Xiaowen Wang,Yunchao Long,Guangming Liu,Guangzhao Zhang
The conformation of polyzwitterionic brushes plays a crucial role in the adsorption/desorption of proteins on solid surfaces. By use of quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR), we have systematically investigated the conformational behavior of poly(sulfobetaine methacrylate) (PSBMA) brushes as a function of ionic strength in the presence of different ions. The frequency change demonstrates that the effectiveness of anions to weaken the inter/intrachain association and to enhance the hydration of the grafted chains increases from kosmotrope to chaotrope in the low ionic strength regime, but the ordering of anions is almost reversed at the high ionic strengths. The dissipation change indicates that some heterogeneous structures are formed inside the brushes in the presence of chaotropic anions with the increase of ionic strength. In SPR studies, the change of resonance unit (ΔRU) with ionic strength is determined by the balance between the increase of thickness and the decrease of refractive index of the brushes. No anion specificity is observed in the SPR measurements because ΔRU is insensitive to the coupled water molecules inside the brushes. For the control of protein adsorption/desorption, our studies show that the brushes can more effectively resist the protein adsorption in the presence of a more chaotropic anion and a more chaotropic anion can also more effectively induce the protein desorption from the surface of the brushes. In addition, no obvious cation specificity can be observed in the conformational change of the brushes in either QCM-D or SPR measurements.
研究领域:
材料科学
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生物物理学
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表面化学
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高分子科学
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DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Ning Xia,Ting Sun,Lin Liu,Linxu Tian,Zhifang Sun
Heterogeneous analysis has great application prospects in the detection of post-translational modification (PTM) enzymes with the advantages of signal enhancement, less sample demand, and high sensitivity and selectivity . Nevertheless, once the substrate was fixed on a solid interface, the steric hindrance might limit the approaching of catalytic center to the substrate, thus reducing the efficiency of PTM. Herein, we suggested that the avidin-modified interface could be used to develop heterogeneous sensing platforms with biotin-labeled substrates as the probes, in which the enzymatic PTM was performed in solution and the heterogeneous assay was conducted on a solid surface. The sensing strategy integrates the advantages but overcomes the defects of both homogeneous and heterogeneous assays. Protein kinase A (PKA) and histone acetyltransferase (HAT) were determined as the examples by using sequence-specific peptide substrates. The signal changes were monitored by HRP-based colorimetric assay and antibody-amplified surface plasmon resonance (SPR). The methods were used for analysis of cell lysates and evaluation of inhibition efficiency with satisfactory results. The strategy can be used for the detection of a variety of biological enzymes and provide a new idea for the design of various heterogeneous biosensors. Thus, this work should be of great significance to the popularization and practical application of biosensors.
研究领域:
生物化学
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分子生物学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
Advanced Optical Materials
·
2022
·Vol. 11
·期 1
·2202115
·
IF 10.05
Shengliang Zhang,Yutao Peng,Jing Zhao,Zengjie Fan,Bing Ding,Jim Yang Lee,Xiaogang Zhang,Yimin Xuan
Dual-band electrochromic smart windows with dynamic and independent control of near-infrared (NIR) and visible (VIS) light transmittance can notably reduce the carbon footprint of buildings and improve the occupants’ visual and thermal comfort. The dual-band response is often generated by the phase transition of crystalline electrochromic materials. However, the phase-transition of crystalline materials is kinetically sluggish, resulting in a long response time. Herein, a fast-switching dual-band electrochromic smart window based on a single-component amorphous and porous tungsten oxide (AP-WO 3 ) cathode without the slow phase-transition process is demonstrated. The amorphous and porous structure of WO 3 not only significantly improves the ion transport, but also provides a large surface area for Li + adsorption that gives rise to a tunable surface plasmon resonance (SPR) in the NIR range. Consequently, the single-component AP-WO 3 film can modulate the NIR and VIS light transmittance independently and effectively through three distinct modes with high optical modulation, fast switching speed, high bistability, and good cycling stability. The prototype dual-band electrochromic device assembled by AP-WO 3 cathode also delivers an impressive dual-band electrochromic performance with fast-switching speed (6.4 s for coloration and 7.2 s for bleaching).
研究领域:
材料科学
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电化学
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光电技术
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能源效率
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建筑技术
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产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2026
·
IF 5.1
Qingyuan Wang,Huiqian Zhang,Feilu Wang,Yubin Xu,Zixuan Zheng,Shaowei Wang,Ren-shan Ge,Xiaoheng Li
Quaternary ammonium compounds, including benzalkonium chlorides (BACs), have broad uses as disinfectants, preservatives, and surfactants. The escalating application of QACs as disinfectants post-COVID-19 raises significant public health concerns regarding their potential as endocrine disruptors. To investigate this, a systematic literature review was conducted using PubMed, Web of Science, and Scopus databases for the period 2000–2026, identifying a critical gap regarding BAC's effects on glucocorticoid metabolism. The current study therefore aims to explore the suppressive effects of nine BACs on human and rat 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), a steroidogenic enzyme for glucocorticoid inactivation, in human and rat microsomes (placental and renal sources) using HPLC-MS/MS technique and Surface Plasmon Resonance (SPR) assays. BACsblocked 11β-HSD2 depending on alkyl group, with C10 + derivatives exhibiting marked activity for human and C12 + derivatives for rat enzyme. C16 BAC emerged as the strongest suppressor (IC 50 = 4.22 μM against human enzyme; 24.74 μM against rat homolog). SPR assay showed that C16 BAC had a high affinity binding with KD of 1.95 μM while C18 had moderate affinity with KD of 19.5 μM against human 11β-HSD2. Mechanistic studies identified BACs as mixed/noncompetitive inhibitors of 11β-HSD2 in both species. In intact human BeWo cells, C10-C18 BACs effectively suppressed cortisol metabolism at 1–100 μM. Pearson correlation analysis linked inhibitory potency to structural features (LogP, molecular weight, alkyl carbon count, heavy atoms, flex, Fsp 3 , volume, Sterimol L) for both enzymes. A 3D-QSAR highlighted apolar interactions are critical. Molecular docking further elucidated binding mechanisms, showing that BACs occupy the NAD + -binding site via van der Waals, H-bonds, apolar contacts, and charges. Collectively, this study reveals a novel mechanism by which BACs disrupt glucocorticoid metabolism through inhibiting 11β-HSD2. The findings underscore BACs as potent environmental endocrine disruptors, warranting urgent re-evaluation of their safety guidelines .
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DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 9
·967-972
·
IF 1.618
Wang Wen-Wen,Han Xiao,Chu Li-Qiang
Nucleic acid aptamers have been widely used as synthetic probes for bioanalytical applications. Herein, we carried out a detailed study on the immobilization of a series of aptamers ranging from 37 to 88 bases, which are specific to either Escherichia coli ( E. coli ) or Staphylococcus aureus ( S. aureus ), on a planar gold substrate via a polyadenine-mediated immobilization method. The resultant surfaces were characterized by both surface plasmon resonance spectroscopy (SPR) and X-ray photoelectron spectroscopy. The results clearly show that the aptamer solution at a lower ionic strength gives rise to a higher lateral density of the aptamer when compared to that at a higher ionic strength. The SPR aptasensors are then employed for detecting their corresponding bacteria ( i.e., E. coli and S. aureus , respectively). The data indicate that the SPR aptasensor with a higher density of aptamer exhibits a better capture of target bacteria.
研究领域:
生物化学
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分析化学
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生物传感器
·
微生物学
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DOI
PubMed
高影响因子
SLAS Discovery
·
2024
·
IF 2.7
Cui Lin,Yijie Wang,Tao Peng,Pengpeng Liu,Yuanyuan Liang,Wencheng Kang,Xiaoping Yu,Yang Song,Xuping Shentu
Neuron-specific enolase (NSE) is currently the most reliable biomarker for small cell lung cancer (SCLC), which is important for disease monitoring, clinical evaluation and diagnosis. However, traditional methods suffer from various disadvantages, including instability, complexity, time-consuming operations, and the necessity for standards. In this study, we developed a calibration-free concentration analysis (CFCA) method based on surface plasmon resonance (SPR) technology, to accurately quantify the active concentration of NSE without relying on any standards. Based on the principle of CFCA, the active concentration of NSE can be calculated by observing binding rate variations at two flow rates under partial mass transport limitation and combining it with the known diffusion coefficient of the NSE. Using the method of CFCA, the active concentration of NSE was determined was only 0.48 mg/mL with an intra-day repeatability of 4.75%. The method has the advantages of simplicity, rapidity, realistic analysis and ease of implementation of high-throughput automated detection. Therefore, the method is expected to become the main measurement method for protein active concentration, which will be beneficial for the development of active protein standards.
研究领域:
肿瘤学
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生物标志物研究
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分析化学
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生物物理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Shuguang Li,Jize Liu,Haibo Wu,Xinxin Zhang,Yanqiong Guo,Boyang Kang,Limin Zhao
Photocatalytic hydrogen production with simultaneous degradation of organic pollutants by using solar energy provide a promising strategy to solve the energy and environmental issue in the future. However, for onefold wide band-gap semiconductor, the poor photocatalytic efficiency limits its application on a large scale due to its easy recombination of photo-generated electron-hole pairs and low solar energy utilization. Herein, a novel Z-scheme BiVO 4 (040)-Bi-(NaYF 4 :Er,Yb,Tm@BiOBr)/Bi photocatalyst is prepared via photo-assisted isoelectric point method. In this composite photocatalyst, up-convention luminescence material, NaYF 4 :Er,Yb,Tm, can offer more ultraviolet light and visible light to intensify the activations of BiOBr and metal Bi nanoparticles, respectively. Furthermore, the formation of Z-scheme BiVO 4 (040)-BiOBr photocatalystic sytem, the spatial separation of the BiVO 4 (040) and BiVO 4 (110) facets and the presence of metal Bi surface plasmon resonance effect are contributed to the high-efficiency separation of the photo-generated electron-hole pairs. The experimental results show that as-prepared Z-scheme BiVO 4 (040)-Bi-(NaYF 4 :Er,Yb,Tm@BiOBr)/Bi photocatalyst exhibits advanced photocatalytic hydrogen production amount (192.0 μm) and excellent degradation ratio of malachite green (88.51 %) under simulated sunlight irradiation for 5.0 h. The apparent quantum yield of the Z-scheme BiVO 4 (040)-Bi-(NaYF 4 :Er,Yb,Tm@BiOBr)/Bi photocatalyst at 420 nm reaches a specific value of 5.8 %. In addition, Z-scheme BiVO 4 (040)-Bi-(NaYF 4 :Er,Yb,Tm@BiOBr)/Bi photocatalyst also remains a high stability in the photocatalytic hydrogen production with simultaneous degradation of malachite green within six reuse. At last, the possible reaction mechanism and degradation pathways of malachite green caused by Z-scheme BiVO 4 (040)-Bi-(NaYF 4 :Er,Yb,Tm@BiOBr)/Bi photocatalyst under simulated sunlight irradiation are put forward.
研究领域:
材料科学
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环境科学
·
化学工程
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DOI
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Baichuan Zhao,Huihui Liu,Lingzhi Xia,Zhi Wang,Congyun Zhang
The development of powerful technologies to simultaneously monitor and eliminate toxic organic pollutants is a vital focus of environmental research. For this purpose, a recyclable surface-enhanced Raman scattering (SERS) substrate was successfully prepared by successively depositing gold nanoparticles (AuNPs) and a reduced graphene oxide (rGO) film on TiO2 microflower particles by two-step in situ reduction processes. The prepared TiO2@Au-rGO ternary composites exhibit not only good SERS performance via molecule enrichment of rGO and surface plasmon resonance (SPR) capability, but also enhanced photocatalytic degradation activity because of the increased broadband UV–visible-light-harvesting efficiency and SPR-enhanced charge transfer. The detection limit of rhodamine 6G (R6G) as low as 10–11 M and excellent reproducibility were achieved. Additionally, the TiO2@Au-rGO ternary composites yield complete removal of R6G within 15 min under broadband UV–visible light illumination, wherein their apparent reaction-rate constant is 2.8 and 5.5 times larger than those of binary TiO2@Au and pure TiO2, respectively. Meanwhile, no distinct SERS and photocatalytic activity loss were observed even after five reusability tests, achieving the recycling SERS application by photocatalytic degradation. Most importantly, the as-fabricated ternary samples can be explored for the efficient capture, sensitive detection, and self-cleaning degradation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), revealing great potential applications in the SERS-based simultaneous monitoring and removal of trace aromatic persistent organic pollutants.
研究领域:
环境科学
·
材料科学
·
分析化学
·
纳米技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Kaijie Ma,Le Liu,Pengfei Zhang,Yonghong He,Qing Peng
An angular plasmonic sensor based on optimized angle-pixel resolution to detect antibody-antigen binding process is presented. Owing to discrete resonance curves by the CCD pixels, the pixel sensitivity and detection noise of the plasmonic sensors need to be balanced. The angle-pixel resolution is adjusted by changing the distance between the sensor chip and the CCD to obtain the optimal refractive index (RI) resolution for three plasmonic sensors, which are surface plasmon resonance (SPR), long range surface plasmon resonance (LRSPR) and plasmon waveguide resonance (PWR) sensors. Each plasmonic sensor can achieve its optimal RI resolution at the optimal distance, and the LRSPR sensor has the best RI resolution. Attributing to the reference channel and temperature control module in the sensor system, the RI resolution can achieve 4.37 × 10 −7 RIU at Normal mode and 1.13 × 10 -7 RIU at a slow response Extreme mode. The Protein A-IgG binding reaction experiment indicates that the plasmonic sensor has linear response to IgG concentration in a wide range and the limit of detection (LOD) is 5.45 pM at Extreme mode. The procedure and result of this work are helpful to the optimization of other angular plasmonic sensors.
研究领域:
生物传感
·
等离子体学
·
光学传感
·
分析化学
·
生物物理学
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产品关联
DOI
高影响因子
Journal of the American Chemical Society
·
2025
·
IF 15.6
Pei Liu,Mengmeng Zheng,Yue Wang,Xiang-Yu Kong,Yingjie Zhao,Mengru Zhang,Qun Xu,Lei Jiang,Liping Wen
The process of photosynthesis in green plants and energy conversion in certain archaea are inseparable from photoregulated ion pumping. Two-dimensional (2D) nanofluidic membranes, emerging as competitive candidates for constructing ion pumps, present intriguing prospects for harvesting light energy. However, the inevitable presence of defects during the synthesis of 2D materials underscores the critical need to understand their impact on ion transport dynamics within nanofluidic systems. Here, we present a chemically engineered asymmetric nanofluidic membrane (ANM) by intercalating molybdenum trioxide (MoO3) with controlled oxygen vacancies into graphene oxide laminates, systematically investigating the defect chemistry-governed photo-activated ion transport. Upon photoexcitation, MoO3 nanosheets exhibit tunable surface plasmon resonance (SPR) through photochemical H+ intercalation, leading to an augmentation of oxygen vacancies and an elevated concentration of free electrons. These vacancies enrich MoO3 with excess localized surface electrons, enabling tunable SPR that creates negative charge centers and significantly enhances space charge through defect-induced polarization. Density functional theory (DFT) calculations reveal the atomic-level mechanism of vacancy-enhanced cation transport, showing a notable 188% increase in the adsorption energy of K+ at MoO3 surfaces with two vacancy sites compared to one vacancy (−13.66 vs −4.74 eV). Under asymmetric photo irradiation, the system achieves a peak power density of approximately 439.2 W/cm2 in equilibrium ionic solutions, realizing a photonic-to-ionic energy conversion efficiency of 7.98 × 10–4% via synergistic effects of defects and plasmonics. Our research pioneers the elucidation of the regulation and underlying mechanisms of intrinsic defects on active ion transport within nanofluidic membranes, while fostering a novel perspective on photon–electron–ion interplay within nanofluidic environments.
研究领域:
纳米流体学
·
能量转换
·
材料科学
·
物理化学
·
光子学
·
电化学
·
凝聚态物理
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产品关联
DOI
PubMed
高影响因子
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