SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Mingxia Jiang,Yuyin Lin,Liyuan Xu,Zhihang Chen,Junxian Wu,Yong Yuan,Lihua Zhou
Extracellular respiratory bacteria (ERB) assume a significant role in biological and environmental remediation, so the rapid detection of ERB is essential to monitor its biological repair process. This study developed a microfluidic colorimetric biosensor for rapid and sensitive detection of ERB model strain Shewanella oneidensis MR-1. In the process of separation and enrichment of bacteria based on immune magnetic nanoparticles (MNPs), the biological signal is significantly amplified by the excellent peroxidase activity of flower-like CoOOH (FL-CoOOH). Firstly, immune MNPs, samples and immune FL-CoOOH were mixed and incubated within the microfluidic chip’s channel. Magnetic capture in the separation chamber yielded MNP-bacteria-CoOOH complex. Then, 3, 3′, 5, 5′-tetramethylbenzidine (TMB) substrate was added to make the FL-CoOOH nanozyme catalyze the formation of yellow product. Finally, the target bacteria in the microplate were quantitatively detected. Based on the excellent simulated enzyme activity and stability of FL-CoOOH, the detection can be completed within 45 min after the introduction of the microfluidic chip. The detection was 5×10 1 ∼5×10 6 CFU/mL. Compared with the previously reported colorimetric sensors for MR-1, the sensitivity was increased by 99.54 % to 23 CFU/mL. This study uniquely integrates optical sensors and microfluidic technology for rapid ERB detection with enhanced portability. The design of biosensor can facilitate further investigations into extracellular respiration mechanisms and rapid ecological environment restoration applications.
研究领域:
生物技术
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环境科学
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分析化学
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微流控
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2024
·
IF 1.2
Guofen Zhou,Linlin Yu,Wenlong Guo,Zhongwei Jiang,Lianzhe Hu,Min Wang
In this study, nanozyme is used for monitoring the dose of ultraviolet (UV) in sunlight for the first time. We find that nitrogen-doped carbon dots (N-doped C-dots) can effectively catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) under different UV region including UVA, UVB, and UVC. In comparison, the N-doped C-dots fail to show the catalytic activity under visible light. The finding is applied for distinguishing UV light and visible light through the color change of TMB. More importantly, the photo-oxidation TMB catalyzed by N-doped C-dots can work well in a near neutral pH, which is beneficial for its application in wearable device due to the reducing of acid corrosion. Finally, a wearable bracelet is fabricated by integrating N-doped C-dots and TMB into hydrogels. The bracelet is used for monitoring the UV exposure in sunlight. The color change of TMB in the bracelet can be clearly observed by naked eyes or quantified by a smartphone. The bracelet is also used for distinguishing different weather condition such as sunny/cloudy or different place such as indoor/outdoor during a sunny day. This study provides the first example of nanozyme in wearable UV sensor and will stimulate the applications of nanozyme in wearable device.
CAS / MDL:
研究领域:
材料科学
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纳米技术
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可穿戴技术
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环境监测
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光化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 5
·1-8
·
IF 5.479
Zhao Chen,Hong Cheng-yi,Lin Zheng-zhong,Chen Xiao-mei,Huang Zhi-Yong
A specific and sensitive colorimetric aptasensor is described for the determination of Malachite Green (MG). It is exploiting the inhibition of the peroxidase-like activity of gold nanoparticles (AuNPs). The AuNPs act as enzyme mimics that catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to yield a dark blue solution. The catalytic activity is inhibited by hexadecyl trimethyl ammonium ion, specifically by cetyltrimethylammonium bromide (CTAB), which causes the aggregation of AuNPs. If a (negatively charged) RNA-aptamer against MG is added, it binds to the positively charged CTAB and prevents aggregation. This enhances the enzyme mimicking activity of the AuNPs and leads to the formation of a dark blue solution. However, in the presence of MG, the aptamer binds to MG, and leads to the aggregation of AuNPs again. The aggregated AuNPs possess a light blue color. A colorimetric method (best performed at 650 nm) was work out that can detect MG in a concentration range from 10 to 500 nmol L −1 . The detection limit based on 3σ/k criterion is 1.8 nmol L −1 . The assay is highly specific and accurate. Recoveries from spiked real samples (aquaculture water) ranged from 80% to 120%.
Based on the inhibition of cetyltrimethyal ammonium ion and the enhancement of RNA-aptamer, the differences of the peroxidase-like activities of AuNPs can be greatly enlarged with and without MG, by which a colorimetric aptasensor can be constructed for the detection of Malachite Green (MG).
研究领域:
分析化学
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纳米技术
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环境科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Hang-Yu Zhou,Tong-Qing Chai,Li-Jing Peng,Wei-Yi Zhang,Tao Tian,Hao Zhang,Feng-Qing Yang
A bisubstrate colorimetric system was developed for rapid and multi-color assay of copper ion (Cu 2+ ). Cu 2+ -triethylamine (Cu 2+ -TEA) complex with the peroxidase-like activity can catalyze the 3,3′,5,5′-tetramethylbenzidine (TMB) to produce oxidized TMB (TMB + , color in blue) and dopamine (DA) to oxidized DA (oxDA, color in orange) in the presence of hydrogen peroxide, which can obtain a three-color colorimetric result due to the couple of complementary colors of blue and orange. The determination of Cu 2+ is achieved based on the absorbance value at 460 nm of the composite peak of TMB + and oxDA. After systematically optimizing the reaction conditions, the developed bisubstrate colorimetric method exhibits high sensitivity for the detection of Cu 2+ (limit of quantitation (LOQ) is 0.0625 μM) with a wide linear range of 0.0625–8.0 μM (R 2 = 0.991). Meanwhile, the reaction solution shows an orange, brown, and green color change with the increase in Cu 2+ concentration, which can realize the semi-quantitatively detection by the naked eyes. Furthermore, TMB was also used as a single substrate for Cu 2+ detection with a linear range of 0.5–9.0 μM (R 2 = 0.994), and the LOQ is determined to be 0.5 μM. The LOQ of bisubstrate system is 8 times lower than that of single substrate system, indicating a higher sensitivity for the detection of Cu 2+ using the bisubstrate colorimetric system. In addition, the developed bisubstrate colorimetric method exhibits excellent selectivity for Cu 2+ detection, which was applied in the determination of Cu 2+ in lake water and soil samples, and satisfactory spiked recoveries were obtained.
研究领域:
分析化学
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环境科学
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光谱学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Danni Wang,Rui Guo,Yunyun Wei,Yingzhi Zhang,Xiayu Zhao,Zhangrun Xu
Detection of telomerase activity is crucial for the telomerase-related early diagnosis of cancer and drug screening. Herein, a multicolor visual telomerase detection method with high sensitivity was developed based on the etching of hexadecyl trimethyl ammonium bromide-stablized Au nanorods (Au NRs) by the oxidation state 3,3′,5,5′-tetramethylbenzidine sulfate (TMB 2+ ). In order to meet the demand of bare-eye inspection, an enzyme-free signal amplification strategy of catalytic hairpin assembly (CHA) was incorporated. After the introduction of telomerase, telomerase extension products specifically triggered cyclic CHA and led to the successive formation of G-quadruplex/hemin DNAzyme, which catalyzed the H 2 O 2 -mediated oxidation of TMB. The Au NRs were gradually etched as the concentration of catalysate TMB 2+ increased, resulting in the decrease of aspect ratio of the Au NRs. Correspondingly, the longitudinal localized surface plasmon resonance peak of Au NRs was blue-shifted, with the concomitant generation of a series of color transition. Under optimal conditions, a highly sensitive detection toward telomerase was realized down to 15 HeLa cells . Compared to previous colorimetric method for telomerase determination, multiple colors corresponding to telomerase activity was the most attractive virtue of our approach. More strikingly, telomerase-induced cyclic CHA significantly enhanced the accumulation of G-quadruplex/hemin DNAzyme, thus the signal amplification was effectively realized. Our approach exhibited excellent sensitivity and convenient signal readout, which is expected to provide great potential application in cancer diagnosis and therapy.
研究领域:
癌症生物学
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纳米技术
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-10
·
IF 6.408
Zhou Xuemei,Qi Yuji,Tang Yulian,Gao Hui,Lv Li,Lei Xingyu,Hu Lei,Yan Zhengquan
To enhance the peroxidase-like performance and its application in detection of toxic o -aminophenol ( o -AP), a kind of bimetal Cu–Zn oxide-based mesoporous nanosphere (Cu 2/3 Zn 1/3 O PNPs) was constructed under microwave-radiation conditions. Its mesoporous microstructure and peroxidase-like catalytic activity were investigated in detail. The results showed that Cu 2/3 Zn 1/3 O PNPs possessed a high specific surface area of 34.89 m 2 g −1 and a well-distributed mesoporous size of approximate 6.07 nm, which endowed the superior peroxidase-like performance. The material catalyzes the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB) with K m / V max of 0.104 mM/3.79 × 10 −8 M·s −1 in the presence of H 2 O 2 . Especially o -AP could exclusively deteriorate the characteristic UV–Vis absorbance intensity at 653 nm ( A 653 ) of the Cu 2/3 Zn 1/3 O PNPs-TMB-H 2 O 2 system with obvious color change from blue to colorless. Under the optimal conditions, the effect of some interfering substances was low and the limit of detection (LOD) for o -AP was 1.65 × 10 −8 mol/L (S/N = 3). When applied to the colorimetric detection of o -AP in practice, the recovery was between 96.1 and 107.2% with R.S.D. less than 2.04%. The mechanism of synergic-enhancement peroxidase-mimic activity of Cu 2/3 Zn 1/3 O PNPs and its exclusive colorimetric response to o -AP were proposed as well. Graphical abstract
研究领域:
纳米技术
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Yinhui Yi,Xun Zhou,Diyan Liao,Jieling Hou,Hongde Liu,Gangbing Zhu
The sensitive detection of acetylcholinesterase (AChE) and organophosphorus pesticides (OPs) is very important for the protection of human health. Herein, a hybrid material, Pt NPs/Fe-MOF, consisting of a metal–organic framework (MIL-88B-NH2, Fe-MOF) decorated with platinum nanoparticles (Pt NPs), was prepared first and exhibited remarkably improved and excellent peroxidase-mimicking activity compared to the Fe-MOF material resulting from the synergistic catalysis effect between Fe-MOF and Pt NPs, which can effectively catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to generate a blue product (oxidized TMB, oxTMB). Interestingly, in the presence of AChE and acetylcholinesterase, the peroxidase-mimicking activity from Pt NPs/Fe-MOF was inhibited obviously, and thus, a colorimetric sensing platform for AChE can be constructed; more importantly, after the addition of OPs, this nanozyme activity can be recovered, inducing the further successful construction of a sensitive colorimetric sensing platform for OPs. The related sensing mechanism and condition optimization were studied, and the as-prepared Pt NPs/Fe-MOF nanozyme-based colorimetric method for AChE and OP detection displayed superior analytical performances with wide linearities and low detection limits. Furthermore, the designed method offers satisfactory real application ability. We expect the as-proposed Pt NPs/Fe-MOF nanozyme-based colorimetric sensing platform for AChE and OPs via the enzyme cascade reaction to show great potential application.
研究领域:
分析化学
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纳米技术
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环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ying Li,Yuyang Zhang,Rida Javed,Rui Li,Hongbin Zhao,Xing Liu,Chenghui Zhang,Hongmei Cao,Daixin Ye
Recently, nanozymes as an outstanding alternative to natural enzymes has attracted wide attention because of its high stability performance. In this study, P N C nonmetal nanozymes with high oxidase-like activity was synthesized can specifically catalyze colorless 3,3,5,5-tetramethyl-benzidine(TMB) to form blue oxidized TMB (TMBox). In the presence of nitrite, it further oxidizes TMBox to obtain yellow derivative products attributed to nitrite inducing diazotization reaction in TMBox. Based on this principle, a colorimetric and electrochemical sensing system was developed, and the ultra-sensitive multi-mode detection of nitrite was realized by combining RGB mode of smart phone, UV–Vis spectrum and electrochemical method. Compared with single signal detection, the multi-mode sensing system can realize self-validation to achieve more reliable detection results. What's more, the developed multi-mode sensing could quickly and sensitively detect nitrite in real sample, especially RGB mode of smart phone meeting the equipment limited areas, suggesting a broad application prospects in food safety.
研究领域:
分析化学
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材料科学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Lili Zhao,Jiawei Zhou,Peipei Li,Shu Huang,Xiaohua Zhu,Youyu Zhang,Meiling Liu,Shouzhuo Yao
Human immunoglobulin G (HIgG) plays a significant role in diagnosing infections and monitoring autoimmune diseases. However, the development of sensitive and reliable sensing platforms remains a challenge. Based on the Ag 2 S/SnO 2 composites, a dual-mode sensing platform was developed using multifunctional CoOOH nanoflakes for the sensitive detection of HIgG through photoelectrochemistry (PEC) and colorimetry. In the PEC mode, Ag 2 S QDs boost the PEC activity of SnO 2 NFs and facilitate the subsequent antibody immobilization. Ascorbic acid (AA) captures holes in Ag 2 S/SnO 2 to enhance the photocurrent. CoOOH, acting as a secondary antibody label, forms a sandwich structure with the electrode. The loading of HIgG and CoOOH-Ab2 due to specific recognition of antigen-antibody, leads to the quenching of PEC signal. Various amounts of HIgG will bind with different amounts of CoOOH-Ab2, displaying a linear response within the range of 100 pg/mL∼10 μg/mL, with the detection limit of 2.2 pg/mL. In the colorimetric mode, CoOOH efficiently catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), and various amount of the sandwich biocomplexes formed with different HIgG levels lead to distinct color changes for visual detection. The linear response range is 500 pg/mL∼9.375 μg/mL with a lower detection limit of 0.29 ng/mL. The two independent modes with different mechanisms, based on the same PEC electrode, can validate each other and provide more scientific and reliable results in response to the targets. Thus, this dual-mode sensing platform for targets-induced signaling changes presents a novel strategy for bioanalysis and detection.
研究领域:
生物化学
·
分析化学
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纳米技术
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Sijie Liu,Leina Dou,Xiaolin Yao,Wentao Zhang,Man Zhao,Xuechi Yin,Jing Sun,Daohong Zhang,Jianlong Wang
Development of sensitive, facile and rapid biosensors is important for widespread applications. Nanozymes can be ideal signal donors for constructing dual-readout lateral flow immunoassays (LFIA) because they are an excellent class of optical reporters. Herein, a magnetic prussian blue nanozyme (MPBN) mediated dual-readout on-demand multiplex lateral flow immunoassay (MLFIA) was established by employing ractopamine (RAC) and clenbuterol (CLE) as the model analytes . The MPBN was synthesized through in-suit shell-growing and introduced as a bifunctional signal tag owing to their darker original color and peroxidase-like activity. Based on the catalytic signal created by catalyzing oxidation of chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) and colorimetric signal generated by tag's original color, improved precision and broadened detection range were acquired by implementing a dual-readout strategy. And a two-fold increase in the detection range could fulfill different limit requirements of the same target in various regions. The obtained recoveries from 84.01% to 119.94% indicating the repeatability and reliability of the proposed method. This method provides an attractive platform for the detection of a same target with different detection limits, which possesses a considerable potential in monitoring of other targets.
研究领域:
分析化学
·
生物传感
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纳米技术
·
免疫测定
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yingxia Zhou,Lai Wei,Lihua Chen
Herein, a small molecule, hemin/G-quadruplex (G4-hemin), was physically embedded into NH 2 -MIL-53 (Al) to construct a novel metal-organic framework (MOF)-based enzyme ( [email protected] ). Its catalytic activity and stability were investigated in detail by catalyzing a reaction of 3,3′,5,5′-Tetramethylbenzidine (TMB) in the presence of H 2 O 2 . The results show that the activity and stability of this new enzyme is better than that of traditional hemin or G4-hemin, and even stronger than that of [email protected] ( [email protected] > G4-hemin > [email protected] > hemin). No significant loss in catalytic activity was observed for at least one day. Even after eight days, only a 13.8 % decrease in activity was recorded. An electrochemical biosensor was successfully developed based on a signal amplification strategy utilizing the catalysis of [email protected] The sensor was able to sensitively detect TB in buffer, even in a diluted solution of many proteins. The proposed method offers high selectivity against mismatch sequences of TB, a good linear response range of 10 −18 moL L -1 to 10 -14 moL L -1 and a low minimum detection limit of 0.330 aM.
研究领域:
生物化学
·
分析化学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 58
·33589-33595
·
IF 3.049
Yun Zhang,Shuyou Shi,Jiajia Xing,Wenqing Tan,Chenguang Zhang,Lin Zhang,Huan Yuan,Miaomiao Zhang,Jinjuan Qiao
In this study, a novel colorimetric sensing platform was developed for the detection of S. aureus using dog immunoglobulin G (IgG) as the capture antibody and chicken anti-protein A immunoglobulin Y labeled with horseradish peroxidase (HRP-IgY) as the detection antibody. Dog IgG labeled with magnetic beads was used to capture S. aureus through the interaction between the Fc region of dog IgG and Staphylococcal protein A (SPA). HRP-IgY was introduced to recognize the residual SPA on the surface of S. aureus and to create a sandwich format, after which a soluble 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was added. A stop solution was utilized to cease the enzymatic chromogenic reaction, and then optical density was read at 450 nm. Under optimal conditions, the proposed method displayed a low detection limit of 1.0 × 103 CFU mL−1 and a wide linear range of 3.1 × 103 to 2.0 × 105 CFU mL−1. This detection method exhibited high specificity against other foodborne bacteria. The recovery rates ranged from 95.2% to 129.2%. To our knowledge, this is the first report to employ dog IgG and chicken IgY as an antibody pair to detect S. aureus. This technique exhibits high application potential for S. aureus monitoring in various kinds of samples.
研究领域:
生物传感器
·
微生物学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）