Biomed Research International
·
2020
·Vol. 2020
·期 1
·5084057
·
IF 2.276
Li Yang,Yiwei Fang,Ronghua Liu,Junwei He
Dendropanax dentiger root is a traditional medicinal plant in China and used to treat inflammatory diseases for centuries, but its phytochemical profiling and biological functions are still unknown. Thus, a rapid, efficient, and precise method based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied to rapidly analyse the phytochemical profiling of D. dentiger with anti-inflammatory and antioxidant activities in vitro . As a result, a total of 78 chemical compositions, including 15 phenylpropanoids, 15 alkaloids, 14 flavonoids, 14 fatty acids, 7 phenols, 4 steroids, 4 cyclic peptides, 3 terpenoids, and 2 others, were identified or tentatively characterized in the roots of D . dentiger . Moreover, alkaloid and cyclic peptide were reported from D . dentiger for the first time. In addition, the ethanol crude extract of D. dentiger roots exhibited remarkable anti-inflammatory activity against cyclooxygenase- (COX-) 2 inhibitory and antioxidant activities in vitro . This study is the first to explore the phytochemical analysis and COX-2 inhibitory activity of D. dentiger . This study can provide important phytochemical profiles and biological functions for the application of D. dentiger roots as a new source of natural COX-2 inhibitors and antioxidants in pharmaceutical industry.
研究领域:
天然药物化学
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药用植物学
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炎症生物学
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自由基生物学
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药物代谢
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DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Dandan Jiang,Xiqian Li,Jiutong Ma,Qiong Jia
In this study, we designed a gadolinium-based immobilized metal ion affinity chromatography material for the selective enrichment of phosphopeptides. Gadolinium ion was immobilized on the surface of glutathione-coated magnetic nanoparticles through a facile and effective synthetic route. The adsorbent integrated the advantages of superparamagnetism of Fe 3 O 4 core, good biological compatibility of glutathione , and strong interaction between gadolinium ion and phosphopeptides. It was employed to enrich phosphopeptides from standard protein digests coupled with MALDI-TOF MS. Results demonstrated that the adsorbent possessed high selectivity for phosphopeptides, good reusability and reproducibility. Moreover, the material provided selective enrichment of phosphopeptides from real samples including non-fat milk digests and human serum. The developed method exhibited high sensitivity (detection limit of 10 fmol), showing great potential in the detection of low-abundance phosphopeptides in biological samples.
研究领域:
生物化学
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分析化学
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医学检测
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DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2018
·
IF 2.869
Min Tao,Li Zhang,Yinlong Guo
A novel dual-channel enzymatic inhibition measurement (DEIM) method was developed to improve the repeatability with light/heavy isotope substrates, producing reliable relative standard deviations (< 3%) by employing acetylcholinesterase (AChE) as the model enzyme. The matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) was adapted for enzyme-inhibited method due to its good salt-tolerance and high throughput; meanwhile, dual-channel enzymatic reactions were performed to improve the repeatability of each well. The acetylcholinesterase inhibition measurement was conducted by mixing the quenched enzyme reaction solution of blank group (with heavy isotope as substrate) and experimental group (with light isotope as substrate), of which the inhibition rate might be affected by isotope effects. Hence, inverse study and Km measurement were implemented to validate the method. The inverse study shows similar inhibition rate (68.9 and 70.3%) and the Km of isotope substrates are analogous (0.139 and 0.135 mM), which demonstrated that the novel method is feasible to AChE inhibition measurement. Finally, the method was applied to herb extracts, half of which exhibit inhibition to AChE. The precise dual-channel enzymatic inhibition measurement (DEIM) method could be regarded as a promising approach to potential enzyme inhibitor screening.
研究领域:
生物化学
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分析化学
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药理学
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DOI
PubMed
高影响因子
ChemCatChem
·
2023
·Vol. 15
·期 20
·e202300793
·
IF 4.5
Guoqing Wang,Miao Jiang,Zhao Sun,Leilei Qian,Guangjun Ji,Lei Ma,Cunyao Li,Hong Du,Li Yan,Yunjie Ding
Graphical A multifunctional heterogeneous Pd-POPs-CF 3 SO 3 H catalyst containing integrated acid and metal sites and anions was synthesized for tandem conversion of fructose to bio-diketones in good yield with an extraordinary TOF. Tandem conversion of biomass to value-added fine chemicals is a significant challenge. For instance, the production of fine chemicals from fructose involves conversion to 5-hydroxymethylfurfural (5-HMF), followed by another reaction and purification. Dual catalyst systems have been used in nearly every study on the tandem conversion of fructose to bio-diketones. Therefore, a sole multifunctional heterogeneous catalyst was developed in this study for the tandem conversion of fructose to bio-diketones, which has not been reported previously. Instrument corrosion and the separation or purification of 5-HMF were avoided using the multifunctional heterogeneous catalyst, which contained integrated active sites of acid, metal, and anions. The multifunctional CF 3 SO 3 H-functionalized porous-organic-polymers(POPs)-supported Pd catalyst (Pd-POPs-CF 3 SO 3 H) was prepared using a series of modifications. A bio-diketone yield of 51.0 % was achieved using Pd-POPs-CF 3 SO 3 H in the tandem conversion of fructose with an excellent TOF of 88.3 h −1 which is much more efficient than catalyst reported in literatures. Pd-POPs-CF 3 SO 3 H could be reused at least three times with stable performance. Control experiments and characterization results proved that the high specific surface area, hierarchical pore structure, abundance of CF 3 SO 3 −1 anions, and proximity of Pd moieties and acid sites (“The closer the better” principle) led to the decent performance for bio-diketone.
研究领域:
催化
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化学工程
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有机化学
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绿色化学
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DOI
高影响因子
Food & Function
·
2025
·
IF 5.4
Jiapan Gan,Jiayan Xie,Xin Xu,Jiahao Ai,Sisi He,Jiafen Zou,Qiang Yu,Hongli Jin,yanfang liu,Xinmiao Liang
ZhangTouHong (ZTH), highly valuable edible-medicinal resource rich in bioactive flavonoids, lack of chemical composition and biological activities studies. In this work, a preparation process was developed to prepare flavonoid multi-components (FMC) from ZTH, which showed high efficiency and specificity, and increased total flavonoid content by 4.62-fold. Based on UPLC- Q-TOF- MS/MS and molecular networks, the FMC in ZTH were identified for the first time, with a total of 48 flavonoid compounds identified, including 3 potential new compounds. Moreover, the verification results of anti-aging activity demonstrated that FMC have excellent antioxidant activity and anti-aging capacity, extending nematodes lifespan by 21.65% and survival time of oxidative stress nematodes by 35.71%. In summary, this study established an efficient and scalable preparation process for preparing bioactive flavonoid components, and for the first time verified the anti-aging effect of ZTH FMC, providing the scientific basis for deep development and industrial application of this characteristic citrus resource.
研究领域:
天然产物化学
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药用植物学
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分子生物学
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抗衰老研究
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2025
·
IF 4.9
Lei Xia,Fang-Jing Liu,Meng-Jie Wang,Han-Bing Zhang,Jing Zhang,Yao Lu,Le-Le Qiu,Xian-Yong Wei,Yun-Peng Zhao
Production of cyclohexanone through selective hydrogenation of phenol under mild conditions has attracted much attention. Herein, a NaOH-modified Pd/WO 3 rich in oxygen vacancies for selective hydrogenation of phenol was prepared through impregnation method. The highest catalytic activity was exhibited on NaOH-Pd/WO 3 with 25 wt % addition of NaOH, and phenol achieved 100 % conversion at 90 °C and 0.7 MPa H 2 for 2 h, with a cyclohexanone selectivity of 95.3 %. The turnover frequency (TOF) of NaOH-Pd/WO 3 is approximately 3.5 times higher than that of the Pd/WO 3 . Detailed catalyst characterization and theoretical calculations reveal that the high activity and selectivity of NaOH-Pd/WO 3 for phenol hydrogenation to cyclohexanone are highly correlated with the concentration of oxygen vacancies in the catalyst. In-situ infrared and Raman analyses reveal that the OH - introduced by NaOH reacted with the lattice oxygen in WO 3 to abstract oxygen from the lattice, generating a significant number of oxygen vacancies. The possible reaction mechanisms for phenol hydrogenation were elucidated through combined density functional theory calculations and isotopic labeling experiments. This strategy of improving catalytic activity and selectivity by regulating the concentration of oxygen vacancies provides a theoretical basis for the design and optimization of industrial catalysts.
研究领域:
催化
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化学工程
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材料科学
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物理化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Ji-Ping Wei,Bin Qiao,Wen-Jun Song,Tao Chen,Fei li,Bo-Zhi Li,Jin Wang,Ye Han,Yan-Feng Huang,Zhi-Jiang Zhou
Rapid and efficient characterization and identification of pathogens at the strain level is of key importance for epidemiologic investigations, which still remains a challenge. In this work, solvothermically Fe 3 O 4 -COOH@MIL-101 composites were fabricated by in situ crystallization approach. The composites combine the excellent properties of both chromium (III) terephthalate (MIL-101) and carboxylic-functionalized magnetite (Fe 3 O 4 -COOH) particles and possess the efficient peptides/proteins enrichment properties and magnetic responsiveness. Fe 3 O 4 -COOH@MIL-101 composites as magnetic solid phase extraction materials were used to increase the discriminatory power of MALDI-TOF MS profiles. BSA tryptic peptides at a low concentration of 0.25 fmol μL −1 could be detected by MALDI-TOF MS. In addition, Fe 3 O 4 -COOH@MIL-101 composites were successfully applied in the selective enrichment of the protein biomarkers from bacterial cell lysates and discrimination of Escherichia coli at the strain level. This work provides the possibility for wide applications of magnetic MOFs to discriminate pathogens below the species level.
研究领域:
生物化学
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微生物学
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材料科学
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DOI
高影响因子
JOURNAL OF MASS SPECTROMETRY
·
2024
·Vol. 59
·期 7
·e5058
·
IF 1.9
Xintong Zhao,Juan Ren,Ze Wang,Xiangfeng Chen
Analysis of noncovalent interactions between natural products and proteins is important for rapid screening of active ingredients and understanding their pharmacological activities. In this work, the intensity fading MALDI-TOF mass spectrometry (IF-MALDI-MS) method with improved reproducibility was implemented to investigate the binding interactions between saponins from Panax notoginseng and lysozyme. The benchmark IF-MALDI-MS experiment was established using N , N ′, N ″-triacetylchitotriose-lysozyme as a model system. The reproducibility of ion intensities in IF-MALDI-MS was improved by scanning the whole sample deposition with a focused laser beam. The relative standard deviation (RSD) of deposition scanning IF-MALDI-MS is 5.7%. Similar decay trends of the relative intensities of notoginseng saponins against increasing amounts of lysozyme were observed for all six notoginseng saponins. The half-maximal fading concentration (FC 50 ) was calculated to quantitatively characterize the binding affinity of each ligand based on the decay curve. According to the FC 50 values obtained, the binding affinities of the six notoginseng saponins were evaluated in the following order: notoginsenoside S > notoginsenoside Fc > ginsenoside Rb1 > ginsenoside Rd > notoginsenoside Ft1 > ginsenoside Rg1. The binding order was in accordance with molecular docking studies, which showed hydrogen bonding might play a key role in stabilizing the binding interaction. Our results demonstrated that deposition scanning IF-MALDI-MS can provide valuable information on the noncovalent interactions between ligands and proteins.
研究领域:
分析化学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Shiye Lin,Haolin Zhang,Jesus Simal-Gandara,Ka-Wing Cheng,Mingfu Wang,Hui Cao,Jianbo Xiao
Quercetin is one of most important flavonoids in foods with multi-benefits for human health. The thermal processing is the main food processing approach. Here, the stability of quercetin in boiling water (100 °C) was investigated by UPLC-Q-TOF-MS-MS. With the increasing boiling time, quercetin gradually degraded, and the initial degradation time is 17.57 min and the half-degradation time is 169.72 min. The degradation mechanisms included oxidation, hydroxylation and nucleophilic attack cleavage. Combining the retention time and characteristic fragment ion information of the corresponding standards, the degraded products of quercetin in boiling water were identified as 3,4-dihydroxyphenylglyoxylate, 1,3,5-trihydroxybenzene, 3,4,5-trihydroxybenzoic acid and 2,4,6-trihydroxybenzoic acid. Moreover, 2,3-dihydro-2,3′,4′,5,7-pentahydroxy-3-oxoflavone, quercetin dimers and quinones were also formed.
研究领域:
食品科学
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分析化学
·
药理学
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Tong Qingheng,Yao Weifeng
Diabetic nephropathy (DN) is the main cause of end-stage renal disease worldwide and a major public issue affecting the health of people. Therefore, it is essential to explore effective drugs for the treatment of DN. In this study, the traditional Chinese medicine (TCM) formula, Zhijun Tangshen Decoction (ZJTSD), a prescription modified from the classical formula Didang Decoction, has been used in the clinical treatment of DN. However, the chemical basis underlying the therapeutic effects of ZJTSD in treating DN remains unknown. In this study, compounds of ZJTSD and serum after oral administration in rats were identified and analyzed using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS). Meanwhile, a semi-quantitative approach was used to analyze the dynamic changes in the compounds of ZJTSD in vivo. UPLC-Q/TOF-MS analysis identified 190 compounds from ZJTSD, including flavonoids, anthraquinones, terpenoids, phenylpropanoids, alkaloids, and other categories. A total of 156 xenobiotics and metabolites, i.e., 51 prototype compounds and 105 metabolites, were identified from the compounds absorbed into the blood of rats treated with ZJTSD. The results further showed that 23 substances with high relative content, long retention time, and favorable pharmacokinetic characteristics in vivo deserved further investigations and validations of bioactivities. In conclusion, this study revealed the chemical basis underlying the complexity of ZJTSD and investigated the metabolite profiling and pharmacokinetics of ZJTSD-related xenobiotics in rats, thus providing a foundation for further investigation into the pharmacodynamic substance basis and metabolic regulations of ZJTSD.
研究领域:
药学
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分析化学
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药理学
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中药学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 14
·2242
·
IF 4.7
Ting Cai,Nan Hai,Peng Guo,Zhi Feng,Yu Zhang,Jing Wang,Zhipeng Yu,Huan Liu,Long Ding
The objective of this study was to investigate the umami characteristics of soy sauce using electronic tongue evaluation and amino acid composition and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI−TOF MS) analysis. The soy sauce peptides were isolated from soy sauce using XAD−16 macroporous resin combined with ethanol solution. The results showed that the soy sauce peptide fraction eluted by 60% ethanol (SS−60%) exhibited a prominent umami taste, and the umami scores were highly positively correlated with the amino acid nitrogen contents of soy sauces. The umami scores of SS−60% were significantly positively correlated with the contents of free amino acids. Especially, Phe showed the highest positive correlation with the umami scores. In addition, five characteristic ion peaks withm/zat 499, 561, 643, 649, and 855 were identified in the peptide mass fingerprinting. Therefore, this study provides new insights into the umami characteristics for the taste evaluation and reality identification of soy sauce.
研究领域:
食品科学
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分析化学
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感官评价
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产品关联
DOI
PubMed
高影响因子
Food Quality and Safety
·
2023
·
IF 5.6
Xu Daokun,Huang Haolun,Liu Zhen,Wang Yumei,Liu Qinan,Jiang Xing,Yang Jun,Ling Rui
Food allergy is a growing health issue worldwide and the demand for sensitive, robust and high throughput analytical methods is rising. In recent years, mass spectrometry–based methods have been establishing its role in multiple food allergen detection. In the present study, a novel method was developed for the simultaneous detection of almond, cashew, peanut and walnut allergens in bakery foods using liquid chromatography–mass spectrometry. Protein unique to theses four ingredients were extracted, followed by trypsin digestion, quadrupole time–of–flight (Q–TOF) mass spectrometry and bioinformatics analysis. Raw data were processed by de–novo sequencing module plus PEAKS DB (database search) module of the PEAKs software to screen peptides specific to each nut species. Thermal stability and uniqueness of these candidate peptides were further verified using triple quadrupole mass spectrometry (QQQ–MS) under multiple reaction monitoring (MRM) mode. Each nut species was represented by four peptides, all of which were validated for label–free quantification (LFQ). Calibration curves were constructed with good linearity and correlation coefficient (r2) greater than 0.99. The limits of detection (LODs) were determined to range from 0.11 mg/kg to 0.31 mg/kg, and were compared with the reference doses proposed by Voluntary Incidental Trace Allergen Labelling (VITAL). The recoveries of the developed method in incurred bakery food matrices ranged from 72.5% to 92.1% with relative standard deviations (RSD) of less than 5.2%. Commercial bakery food samples detection confirmed existence of undeclared allergens. In conclusion, this method shed light on the field of qualitative and quantitative detection of trace levels of nut allergens in bakery foods.
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高影响因子
危险品化学品经营许可证（带存储）