JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Jinzhi Ren,Ying Peng,Luhang She,Lu Yan,Jianjun Li,Caixia Gao,Chao Wang,Yimeng Wang,Xiangping Nie,Xiaowei Zhang
The estuarine ecological environment faces significant threats from contaminants of emerging concern (CECs); yet, the risk posed by CECs to resident organisms remains poorly understood. Here, we employed tiered toxicity testing to investigate the adverse effects and potential mechanisms of tris (1-chloro-2-propyl) phosphate (TCPP) on the early life stages of an estuarine fish, Mugilogobius chulae . TCPP affected the development of M. chulae embryos, including survival, morphology, hatching, and behavior. A concentration-dependent transcriptomic analysis showed that TCPP disrupted 12 neurodevelopment-related KEGG pathways in M. chulae embryos, with five of the 30 % top-ranked pathways related to neurotransmitter signaling. Besides the cholinergic synapse signaling pathway, the glutamatergic signaling pathway (including NMDAR and AMPAR subtypes) may also mediate TCPP-induced neurodevelopmental toxicity. The NMDAR subtype GRIN2B was downregulated at high concentrations. Molecular dynamics simulations revealed a strong interaction between TCPP and GRIN2B, with TCPP binding to the residues Ile153 and Ile188. The results suggest that NMDARs play a crucial role in TCPP-induced neurodevelopmental toxicity toward M. chulae. AOP network analysis predicted that TCPP may impact cognitive functions and memory. Our study provides a novel testing strategy for identifying the mechanisms of toxicity of CECs, a crucial component of ecological risk assessment.
研究领域:
环境毒理学
·
水生生态学
·
分子生物学
·
神经科学
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PubMed
高影响因子
ELECTROCHEMISTRY
·
2015
·Vol. 83
·期 3
·155-160
·
IF 1.033
Yan-qing LI,Ying LUO,Li-jun LI,Hao CHENG,Wen-yi HUANG
In order to establish a simple, sensitive and rapid method for the determination of azithromycin, the gold electrode modified with Silica sol/Nano-Au/PVP/Ru(bpy)32+ was prepared, and the electrochemiluminescence (ECL) method was developed for the determination of azithromycin based on the sensitizing effect of azithromycin on Ru(bpy)32+. Moreover, the electrochemical behaviors and the electrochemical luminescence behaviors of the Ru(bpy)32+ system and the Ru(bpy)32+- azithromycin system were investigated by ECL respectively. The results show that the proposed electrode showed very good electrochemical activity and electrochemiluminescence response to the Ru(bpy)32+- azithromycin system, under the optimum experimental condition, the linearity of azithromycin concentration to ECL intensity was achieved in the concentration of azithromycin from 1.0 × 10−7 to 1.0 × 10−4 mol L−1 and the detection limit (S/N = 3) was 1.33 × 10−9 mol L−1. Through the parallel determination of 1.0 × 10−5 mol L−1 azithromycin for 8 times, the RSD of results was 1.82%, and the recovery ranging from 97.9% to 101.6% was obtained in the detection of azithromycin tabelets and the RSD was 1.36% (n = 5). The proposed method showed high selectivity and sensitivity and simplicity, and it could be applied to the analysis of azithromycin with satisfactory results.
研究领域:
分析化学
·
药物分析
·
生物医学工程
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高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2021
·
IF 3.975
Hao Li,Weijun Zhen,Chengyuan Dong,Ling Zhao
Nano boron nitride-trimethylolpropane tris (3-mercaptopropionate) grafted poly (L-lactic acid) (BN-TTMP-PLLA) was prepared with nonmetallic organic catalyst by thiol-ene click chemistry. By orthogonal experiment and range analysis, the optimum reaction conditions of BN-TTMP-PLLA were as follows: reaction time of 2.5 h, photoinitiator addition amount of 0.04 g, TTMP addition amount of 0.9 g, and mass ratio of PLLA-AA to BN-570 of 1:1. The product was characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, which showed that PLLA was successfully grafted onto BN surface. Furthermore, PLA/BN-TTMP-PLLA nanocomposites were prepared by melt blending with BN-TTMP-PLLA as modifier to enhance the performance of PLA . Compared with pure PLA, the impact strength and elongation at break of PLA/BN-TTMP-PLLA (0.5 wt%) nanocomposites were increased by 1.58 times and 7.34 times, respectively. Molecular dynamics simulation calculation showed that E Interaction between PLA and BN before and after functionalization decreased from −177.44 kcal/mol to −516.42 kcal/mol, which proved that intermolecular interactions between them were strengthened. In addition, PLA/BN-TTMP-PLLA (0.5 wt%) nanocomposites during melting and isothermal crystallization were studied by Rheonaut technology for simultaneous rheometry and FTIR, which demonstrated that the crystallization properties of PLA was clearly improved due to the strong hydrogen bonding effect between BN-TTMP-PLLA and PLA.
研究领域:
材料科学
·
高分子化学
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高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Huihui Lei,Junxia Wang,Yueying Sun,Zhijuan Wu,Xiaofei Wang,Yawei Wang,Xuedong Wang
As a typical organophosphorus flame retardant, tris(2-chloroethyl) phosphate (TCEP) is refractory in aqueous environment. The application of TAP is a promising method for removing pollutants. Herein, the removal of TCEP using TAP was rigorously investigated, and the effects of some key variables were optimized by the one-factor-at-a-time approach. To further evaluate the interactions among variables, the response surface methodology (RSM) based on central composite design was employed. Under optimized conditions (pH 5, [PS] 0 : [TCEP] 0 = 500:1), the maximum removal efficiency (RE) of TCEP reached up to 90.6%. In real-world waters, the RE of TCEP spanned the range of 56%− 65% in river water, pond water, lake water and sanitary sewage. The low-concentration Cl - (0.1 mM) promoted TCEP degradation, but the contrary case occurred when the high-concentration Cl - , NO 3 - , CO 3 2- , HCO 3 - , HPO 4 2- , H 2 PO 4 - , NH 4 + and humic acid were present owing to their prominently quenching effects on SO 4 •- . Both EPR and scavenger experiments revealed that the main radicals in the TAP system were SO 4 •- and •OH, in which SO 4 •- played the most crucial role in TCEP degradation. GC-MS/MS analysis disclosed that two degradation products appeared, sourcing from the replacement, oxidation, hydroxylation and water-molecule elimination reactions. The other two products were inferred from the comprehensive literature. As for acute toxicity to fish, daphnid and green algae, product A displayed the slightly higher toxicity, whereas other three products exhibited the declining toxicity as compared to their parent molecule. These findings offer a theoretical/practical reference for high-efficiency removal of TCEP and its ecotoxicological risk evaluation.
研究领域:
环境科学
·
毒理学
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PubMed
高影响因子
SYNTHETIC METALS
·
2021
·
IF 3.266
Yueqiang Ma,Yuchang Du,Chunxia Li,Yan Zhang,Hongmei Du,Jinsheng Zhao,Junhong Zhang,Yu Xie
In this work, starting from cyclopenta[2,1-b;3,4-b′]dithiophene (CPDT) and tris(thienothiophene) (3TT) as donor units, and diketopyrrolopyrrole (DPP) as the acceptor unit, three new solution-processable donor-acceptor conjugated copolymers (P1, P2, P3) were successfully synthesized through the palladium-catalyzed Stille coupling reaction. The monomer molar feeding ratios (CPDT:DPP:3TT) of the three polymers are 3:2:1, 2:1:1, 3:1:2, respectively, which are named as PCPT-1, PCPT-2, and PCPT-3. For comparison purpose, we also synthesized the binary copolymers between CPDT and DPP (PCP), CPDT and 3TT (PCT), and compared their properties with the ternary copolymers. The optical band gaps of the five polymers were all lower than 1.9 eV, and they all showed abundant color changes. During the oxidation process , PCPT-1 changed from purple to cyan-gray and turned to earthy brown finally, PCPT-2 changed from purple in a neutral state to blue-gray in an oxidized state. Similarly, PCPT-3 turned from purple to cement gray and finally oxidized to light blue. PCP polymer changed from dark green in a neutral state to cement gray in an oxidized state, PCT polymer changed from purple in a neutral state to Light gray purple in an oxidized state. The electrochromic kinetic performance study showed that the response time of the five polymers was about 1 s within a 4-second interval. In the dynamic stability test of 750 cycles (6000 s), the five polymers demonstrated good stability. The contrast retention of polymer PCPT-2 at 570 nm reached to as high as 96.17%.
研究领域:
材料科学
·
化学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Yi Peng,Yang Zheng,Chongli Shi,Chen Wang,Mengyan Huang,Lingjun Zeng,Susu Wang,Xuan Zhou,Yangzhizhe Gao,Jiawei Hong,María Dolores Hernando Guil,Amadeo R. Fernández-Alba,Damià Barceló,Hui Li
Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is an emerging environmental contaminant of concern due to its widespread presence and potential toxicity. Its metabolite, bis(1,3-dichloro-2-propyl) phosphate (BDCPP), plays a critical role in mediating TDCPP-induced toxicities and may exacerbate aging-related processes. In this study, we quantitatively compared the bioconcentration and toxicity of TDCPP and BDCPP in Caenorhabditis elegans. BDCPP exhibited higher environmental persistence and bioaccumulation potential than TDCPP. Exposure to 0.1–1000 μg/L TDCPP and BDCPP increased Oil red O staining (7.88%–55.44%, 6.42%–33.92%), lipofuscin levels (5.44%–35.98%, 3.54%–28.66%), and reduced the mean lifespan (3.09%–10.31%, 1.84%–8.74%). Overall, TDCPP caused greater triglyceride (TG) accumulation, lipofuscin formation, and lifespan reduction compared to BDCPP. TDCPP reduced brood size by 5.30%–18.95%, while BDCPP exposure increased brood size by up to 13.90%. Mechanism investigations revealed that TDCPP and BDCPP induce aging through distinct pathways. The adverse outcome pathways revealed that BDCPP disrupted germline stem cells (GSCs) apoptosis, transmitting reproductive signals through the kri-1-daf-16-lipl-4 signaling cascade, impairing TG hydrolysis and lifespan, while TDCPP acted through a GSC-independent route. These findings underscore the central role of GSCs in BDCPP-induced aging, enhance our understanding of BDCPP as a secondary pollutant, and provide critical data for assessing the ecological and health risks of chlorinated organophosphate esters.
研究领域:
毒理学
·
环境科学
·
分子生物学
·
衰老研究
·
生态毒理学
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Lei Liu,Zhengzhou Wang
This study presents a one-step synthesis of a magnesium amino- tris -(methylenephosphonate) (Mg-AMP)-reduced graphene oxide (Mg-rGO) hybrid involving graphene oxide (GO) reduction and growth in situ of Mg-AMP nanoparticles in the absence of a reducing agent. Mg-rGO was characterized by X-ray diffraction, X-ray photoelectron and Fourier-transform infrared spectroscopies, transmission electronic microscopy, and thermogravimetric analysis (TGA). Mg-rGO was then used to prepare flame-retardant and toughened phenolic (PF) foam. This additive was found to enhance the compressive and flexural strengths of PF foam as well as to reduce its high friability and brittleness. The limiting oxygen index of the foam with 4 phr Mg-rGO (sample PF/4Mg-rGO) increased to 41.5%, compared with the 38% of untreated foam; the peak heat release rate and total heat release of sample PF/4Mg-rGO were decreased by 28.7 and 18.4%, respectively. Also, the total smoke release and peak CO production rate of PF/4Mg-rGO were reduced by 52.5 and 38.1%, respectively. TGA results indicated that Mg-rGO clearly improved the thermal stability of PF foam.
研究领域:
材料科学
·
化学工程
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Hang Liu,Hua Yin,Xiaolong Yu,Minghan Zhu,Zhi Dang
In this work, the amino-functionalized metal-organic frameworks (MIL-88B-NH 2 ) was synthesized, characterized and used as heterogeneous photo-Fenton catalyst for tris-(2-chloroisopropyl) phosphate (TCPP) degradation. The photo-Fenton activity of MIL-88B-NH 2 was investigated on the basis of influence factors, such as initial pH and TCPP concentration, and coexisting impurities. The results revealed that MIL-88B-NH 2 +H 2 O 2 +Vis system exhibited a satisfactory degradation efficiency of TCPP (almost 100%) within 60 min accompanied by a good reusability. Noticeably, the degradation kinetics constant of TCPP by MIL-88B-NH 2 +H 2 O 2 +Vis system was 0.086 min −1 , which was visibly higher than that of MIL-88B+H 2 O 2 +Vis system (0.021 min −1 ) since the addition of amino-functionalized organic linker inhibiting the recombination rate of the photo-generated electron-hole pairs and improving the visible light response. Combined with the characterization, the conversion of Fe III to Fe II could be accelerated by the photo-generated electron from the excitation of Fe-O clusters and NH 2 functionalities, which strengthened the decomposition of H 2 O 2 and formed plenty •OH. Simultaneously, six steady products were validated and potential degradation pathways of TCPP were proposed. It was anticipated that MIL-88B-NH 2 could be considered as a desirable and alternative candidate in the application of heterogeneous photo-Fenton reaction to control the environmental risks caused by organophosphate flame retardants (OPFRs).
研究领域:
环境科学
·
材料科学
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DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Ya-Qi Zhao,Zhi-Peng Li,Shi-Chao Dong,Hao Wang,Yi-Mei Zhao,Lin-Yi Dong,Zhen-Yu Zhao,Xian-Hua Wang
The effective method for trypsin purification should be established because trypsin has important economic value. In this work, a novel and simple strategy was proposed for fabricating micron-sized magnetic Fe 3 O 4 @agarose-benzamidine beads (MABB) with benzamidine as a ligand, which can efficiently and selectively capture trypsin. The micro-sized MABB, with clear spherical core-shell structure and average particle size of 6.6 μm, showed excellent suspension ability and magnetic responsiveness in aqueous solution. The adsorption capacity and selectivity of MABB towards target trypsin were significantly better than those of non-target lysozyme. According to the Langmuir equation, the maximum adsorption capacity of MABB for trypsin was 1946 mg g −1 at 25 °C, and the adsorption should be a physical sorption process. Furthermore, the initial adsorption rate and half equilibrium time of MABB toward trypsin were 787.4 mg g −1 min −1 and 0.71 min, respectively. To prove the practicability, MABB-based magnetic solid-phase extraction (MSPE) was proposed, and the related parameters were optimized in detail to improve the purification efficiency. With Tris-HCl buffer (50 mM, 10 mM CaCl 2 , pH 8.0) as extraction buffer, Tris-HCl buffer (50 mM, 100 mM CaCl 2 , pH 8.0) as rinsing buffer, acidic eluent (0.01 M HCl, 0.5 M NaCl, pH 2.0) as eluent buffer and alkaline buffer (1 M Tris-HCl buffer, pH 10.0) as neutralization solution, the MABB-based MSPE was successfully used for trypsin purification from the viscera of grass carp ( Ctenopharyngodon idella ). The molecular weight of purified trypsin was determined as approximate 23 kDa through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified trypsin was highly active from 30 °C to 60 °C, with an optimum temperature of 50 °C, and was tolerant to pH variation, exhibiting 85 % of maximum enzyme activity from pH 7.0 to 10.0. The results demonstrated that the proposed MABB-based MSPE could effectively purify trypsin and ensure the biological activity of purified trypsin. Therefore, we believe that the novel MABB could be applicable for efficient purification of trypsin from complex biological systems.
研究领域:
生物化学
·
生物技术
·
分析化学
·
蛋白质纯化
·
酶学
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PubMed
高影响因子
Acta Biomaterialia
·
2011
·
IF 4.824
Weifeng Lin,Juan Zhang,Zhen Wang,Shengfu Chen
A new biocompatible silicone comprising a carboxybetaine (CB) ester analogue, 3-methacryloxypropyltris(trimethylsiloxy)silane (TRIS) and an organic silicone macromer (bis-α,ω-(methacryloxypropyl) polydimethylsiloxane) has been developed using photo-polymerisation. Following interfacial hydrolysis of the CB ester, the resulting zwitterionic material became significantly more hydrophilic and exhibited high resistance to both non-specific protein adsorption and bacterial adhesion . Moreover, the stability of these non-fouling properties was dramatically improved by using a slow and controlled rate of ester hydrolysis of the original protective hydrophobic matrix. The subsequent ability to maintain the original optical and mechanical properties of the bare silicone following surface activation makes this material an ideal candidate for preparing contact lenses and other medical devices .
研究领域:
材料科学
·
生物医学工程
·
化学
·
生物材料
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产品关联
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PubMed
高影响因子
ZEITSCHRIFT FUR KRISTALLOGRAPHIE-NEW CRYSTAL STRUCTURES
·
2022
·Vol. 237
·期 6
·1051-1053
·
IF 0.365
Hua-Xiang Zhang
C 33 H 40 CdN 8 O 6 , triclinic,
P
1
‾
(no. 2), a = 10.6468(11) Å, b = 11.6739(9) Å, c = 14.5312(9) Å, α = 93.976(5)°, β = 102.005(7)°, γ = 111.827(8)°, V = 1618.4(2) Å 3 , Z = 2, R
gt ( F ) = 0.0480, wR
ref ( F
2 ) = 0.1102, T = 293(2) K.
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高影响因子
CHIRALITY
·
2021
·Vol. 33
·期 12
·938-950
·
IF 2.437
Shirong Cao,Chunting Xie,Qianyun Ma,Shaoping Wang,Jiayu Zhang,Zhaokun Wang
In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including ( R , S )-ibuprofen, S-ibuprofen, ( R , S )-ketoprofen, S-ketoprofen, S-naproxen, and ( R , S )-loxoprofen sodium. Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition. It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable. The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers. Calibration curve of each enantiomer at the concentration of 5.0–100 ug/ml showed good linearity with the correlation coefficient above 0.9996. Satisfactory recovery (96.54–101.54%), good intra-day precision (RSD 0.52–1.46%), and inter-day precision (RSD 0.13–1.09%) were also obtained. The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers. Quantitative results of the commercial capsules and tablets demonstrated that the difference between the declared and measured values did not exceed 1.52%.
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高影响因子
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