Nutrients
·
2024
·Vol. 16
·期 9
·1395
·
IF 4.8
Xinru Du,Yongheng Yan,Yufeng Dai,Ruijie Xu
Numerous studies have investigated the immunomodulatory effects of yogurt, but the underlying mechanism remained elusive. This study aimed to elucidate the alleviating properties of yogurt on immunosuppression and proposed the underlying mechanism was related to the metabolite D-lactate. In the healthy mice, we validated the safety of daily yogurt consumption (600 μL) or D-lactate (300 mg/kg). In immunosuppressed mice induced by cyclophosphamide (CTX), we evaluated the immune regulation of yogurt and D-lactate. The result showed that yogurt restored body weight, boosted immune organ index, repaired splenic tissue, recovered the severity of delayed-type hypersensitivity reactions and increased serum cytokines (IgA, IgG, IL-6, IFN-γ). Additionally, yogurt enhanced intestinal immune function by restoring the intestinal barrier and upregulating the abundance of Bifidobacterium and Lactobacillus. Further studies showed that D-lactate alleviated immunosuppression in mice mainly by promoting cellular immunity. D-lactate recovered body weight and organ development, elevated serum cytokines (IgA, IgG, IL-6, IFN-γ), enhanced splenic lymphocyte proliferation and increased the mRNA level of T-bet in splenic lymphocyte to bolster Th1 differentiation. Finally, CTX is a chemotherapeutic drug, thus, the application of yogurt and D-lactate in the tumor-bearing mouse model was initially explored. The results showed that both yogurt (600 μL) and D-lactate (300 mg/kg) reduced cyclophosphamide-induced immunosuppression without promoting tumor growth. Overall, this study evaluated the safety, immune efficacy and applicability of yogurt and D-lactate in regulating immunosuppression. It emphasized the potential of yogurt as a functional food for immune regulation, with D-lactate playing a crucial role in its immunomodulatory effects.
研究领域:
免疫学
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营养学
·
药理学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2025
·
IF 14.3
Xianyu Chu,Yanan Wang,Li Jing,Wei Jiang,Yuanyuan Wu,Ming Lu,Bo Liu,Chunbo Liu,Yantao Sun,Guangbo Che
Constructing heterostructures to regulate the electronic structure is an effective strategy for enhancing the oxygen evolution reaction (OER) electrocatalytic activity. Herein, we prepared heterostructured Co 4 S 3 /CoP 3 (CoPS/NFF) electrocatalysts through a one-step phosphorization and sulfuration process. The synthesized electrode drives an overpotential of 190, 272, and 331 mV at 20, 50, and 100 mA cm −2 for OER in 1 M KOH alkaline media, respectively. These values outperformed those of monophase Co 4 S 3 and CoP 3 as well as the majority of transition metal-based catalysts previously reported. Furthermore, the Density Functional Theory (DFT) calculation results show that charge redistribution occurs at CoPS/NFF heterogeneous interfaces, which facilitates charge transfer and improves catalytic activity. The CoPS/NFF with heterostructure optimizes the adsorption strength of oxygen-containing intermediates (*O, *OH, and *OOH) by appropriately adjusting the d-band center energy level, thereby reducing the energy barrier of OER. This work provides a new perspective on rational design strategies for efficient transition metal-based electrocatalysts by inducing d-band center regulation through the construction of heterostructures.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Yao Yu,Xinru Li,Yiping Hou,Min Wei,Yiyun Qian,Yuxin Zhou,Min Yin,Yanqin Jiang,Pingping Song
Gray mold , caused by Botrytis cinerea is an intractable fungal disease that causes extensive damage to agricultural products. In the search for novel antifungal active ingredients, we discovered a linear pyranocoumarin Pd-D-V was effective against B. cinerea in both in vitro and in vivo assays. Furthermore, this study investigated the effects of Ca 2+ and the Ca 2+ -calcineurin signaling pathway on its antifungal activity against B. cinerea . The results indicated that Pd-D-V reduced the concentration of Ca 2+ in the mycelia of B. cinerea ; CaCl 2 , the Ca 2+ channel blocker verapamil , or the calcineurin inhibitor cyclosporin A could affect the sensitivity of Pd-D-V against B. cinerea ; the expression of genes ( Bccch1, Bcmid1, BccnA, Bccnb1, Bcpmc1, and Bcpmr1 ) of the Ca 2+ -calcineurin signaling pathway decreased after Pd-D-V treatment. In summary, Pd-D-V is compound for developing fungicides against B. cinerea . Pd-D-V can reduce intracellular Ca 2+ concentration and disturb Ca 2+ homeostasis . The Ca 2+ -calcineurin signaling pathway is important in the antifungal activity of Pd-D-V against B. cinerea .
研究领域:
植物病理学
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分子生物学
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真菌学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Zhouzhou Li,Zheng Li,Feng He,Wen He,Qixu Chen,Hongqing Wang
Recently, how to improve the photocatalytic uranium removal performance of Graphite-phase carbon nitride (GCN) has always been a hot research topic. In this paper, a new strategy based on p-n junction and D-A structure is proposed to improve the photocatalytic performance. And as a result, a novel cyano modified photocatalytic material NC-GCN/Ag 2 O was designed and prepared for the reduction of U(VI). Interestingly, the material shows better adsorption performance for U(VI) in the dark treatment stage, and its removal of U(VI) is close to 70 % under the initial U(VI) concentration of 20 ppm and the solid–liquid ratio of 0.2 g/L. And the adsorption is in accordance with the Langmuir model and Pseudo second order model. After 30 min of illumination, the removal rate of U(VI) can reach 93.2 %, significantly higher than that of GCN (13.6 %) and NC-GCN 10 (37.7 %). It’s worth mentioning that photocatalytic reduction dominated the adsorption-reduction process for U(VI) removal after light exposure. In addition, after four cycles, the removal of U(VI) from the composite material decreased by only 5 % with no change in the crystal structure, i.e., it has good reusability and stability. Meanwhile, there was no significant interference with other ions except for Cu 2+ and V 5+ , which had a weak interference. Finally, a new mechanism for U(Ⅵ) reduction was proposed.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Puhong Yi,Yue Xu,Hanlin Liu,Yuhua Hao,Mengdan Liu,Zhiqiang Liu,Yaping Xue,Liqun Jin,Yuguo Zheng
2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid (PPO) is a valuable prochiral keto acid for the asymmetric synthesis of the important herbicide L-phosphinothricin (L-PPT). Developing an efficient biocatalytic pathway to meet the increasing demand for PPO is highly attractive. In this study, a robust D-amino acid aminotransferase from Cytobacillus firmus ( Cf DAAT) was screened, which exhibited excellent tolerability to D,L-PPT and showed remarkable specific activity and affinity toward pyruvate (61.9 U/mg, Km = 7.4 mM). A designed co-substrate regeneration system was then introduced to shift the reaction equilibrium forward by coupling with D-amino acid oxidase (DAAO) and catalase (CAT). An efficient whole-cell biocatalyst was obtained by fine-tuning RBS intensity to coordinate the co-expression of the three enzymes. Moreover, the optimum conditions to promote overall conversion efficiency were identified by response surface methodology. Consequently, with only a catalytic amount of pyruvate (3.33 mol%) added in a 1 L reaction system, 200 mM D-PPT (400 mM D,L-PPT) was completely converted in 10 h, resulting in a PPO productivity of 3.33 g⋅L −1 ⋅h −1 , and a notable conversion rate of >99% at 300 mM D-PPT in 16 h. It demonstrated a considerable catalytic level and provided a competitive route for industrial PPO production from D,L-PPT.
研究领域:
生物化学
·
酶工程
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工业微生物学
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产品关联
DOI
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-9
·
IF 4.38
Dong Wenjing,Song Erqun,Song Yang
The acute liver injury (ALI) and hepatic fibrosis caused by the co-treatment of lipopolysaccharide (LPS)/ d -galactosamine (D-GalN) have been extensively studied. However, whether LPS/D-GalN are genotoxic has been left unknown. In this study, male mice were divided into eight groups with eight animals in each group. For acute challenge of LPS/D-GalN, the mice in each group received a combination of LPS/D-GalN via intraperitoneal injection at the dose of 25 μg/kg/250 mg/kg, 25 μg/kg/500 mg/kg, or 50 μg/kg/500 mg/kg body weight. An additional group for chronic administration of test compounds was conducted by i.p. injection of LPS/D-GalN (10 μg/kg/100 mg/kg) every other day for 8 weeks. Saline solution (0.9%) and cyclophosphamide (CTX) (50 mg/kg body weight) given by i.p. injection was used as the negative and positive control, respectively. The results of single cell gel electrophoresis (SCGE) assay indicated that acute exposure of the mice to LPS/D-GalN caused severe DNA damage in hepatic cells, but not in the brain, sperm or bone marrow cells, which evidenced the genotoxicity of LPS/D-GalN administrated in combination. Interestingly, the chronic administration of LPS/D-GalN triggered significant genotoxic effects not only in hepatic but also in brain cells, with negative results in sperm and bone marrow cells. Histopathological examination in the liver and brain tissues revealed changes consistent with the SCGE results. The present study indicates genotoxic potential of LPS/D-GalN co-administered in mice, which may serve as an in vivo experimental model for relevant genotoxic study.
研究领域:
毒理学
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分子生物学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FLUID PHASE EQUILIBRIA
·
2015
·
IF 2.2
Xuzhao Yang,Jun Wang,Hao Song,Wenyuan Zou
Heat capacity of methyl α- d -glucopyranoside (α-MeG) was measured by differential scanning calorimetry at the temperatures ranging from 298.15 to 513.15 K. Melting point, enthalpy and entropy of fusion, enthalpy H T – H 298.15 K , and entropy S T -S 298.15 K were determined. Thermal decomposition of α-MeG was investigated using thermogravimetric analysis under pure nitrogen atmosphere. Ozawa-Flynn-Wall and ASTM models were used to calculate the activation energy and pre-exponential factor. Solubilities of α-MeG in methanol were measured within the temperature range of 293.15–318.15 K by using a synthetic method under atmospheric pressure. The modified Apelblat, λh , Wilson and NRTL models were applied to correlate the experimental data. The calculated results show good agreement with the experimental data and Wilson model is slightly more accurate than modified Apelblat, λh and NRTL models. The dissolution enthalpy, entropy, and Gibbs free energy were calculated by the Van't Hoff equation. The excess enthalpy of the solution can be evaluated by the λh model.
研究领域:
物理化学
·
食品科学
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产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2015
·Vol. 18
·期 7
·2147-2154
·
IF 8.02
Alei Zhang,Cong Gao,Jing Wang,Kequan Chen,Pingkai Ouyang
In this study, we present an efficient affinity adsorption–enzymatic reaction integrated approach to produce N-acetyl-D-glucosamine (GlcNAc) from crude chitin powders (CP). Chitinase was first purified from the crude enzyme mixture by affinity adsorption on the CP substrate, with ∼92.7% recovery of the enzyme achieved within 10 minutes in the presence of 20 g L−1 of CP under optimized conditions. The chitinase then efficiently converted 40 g L−1 of CP to 39.3 g L−1 of GlcNAc in a batch enzymatic reaction for 4 days, achieving nearly 100% conversion of the substrate CP. The product GlcNAc was easily separated from hydrolysates after destaining, concentrating, crystallizing and drying processes with a high purity of 98%. In addition, the chitinase can be conveniently reused for several batches by the affinity adsorption–enzymatic reaction approach, giving an average yield of 29.6 g L−1 of GlcNAc based on 30 g L−1 of CP as the substrate.
研究领域:
生物化学
·
酶工程
✓ 已验证
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 6
·976-984
·
IF 3.1
Hou Huipeng,Tang Shanshan,Wang Wei,Liu Miao,Liang Axin,Xie Bingteng,Yi Yue,Luo Aiqin
I n this work, an electrochemical chiral sensor of a nanowire-like chiral metal-organic framework/multiwalled carbon nanotube-chitosan/glassy carbon electrode( D -MOF/MWCNTs-CS/GCE) was proposed for the enantiorecognition of L / D -tryptophan( L / D -Trp). The asymmetrical spatial structure of D -MOF provides the feasibility for the enantiorecognition of Trp enantiomers. Moreover, differential pulse voltammetry(DPV) was carried out to be the detection method and the DPV peak potential difference(Δ E p ) between L -Trp and D -Trp was referred as the index of the enantiorecognition performance. Several parameters, such as mass ratios and drop-coated volume of MWCNTs-CS, drop-coated volume and concentration of D -MOF, pH and detection temperature of D -MOF/MWCNTs-CS/GCE were optimized for the largest Δ E p value. And the molecular dynamics(MD) simulation was used to elucidate the enantiorecognition mechanism. Furthermore, the proportions of D -Trp( D -Trp%) in Trp mixtures were detected in a good linear relationship with the DPV peak potentials( E p ), and the proposed electrochemical chiral sensor exhibited good reproducibility, stability and enantiorecognition ability. Additionally, the proposed electrochemical chiral sensor( D -MOF/MWCNTs-CS/GCE) has a good application prospect in the fields of biomedicine, clinical diagnosis, chemical production, pharmaceuticals safety and so on.
研究领域:
分析化学
·
生物医学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Yingqing Zhao,Rui Liu,Christian Marcus Pedersen,Zhenzhou Zhang,Zhaohui Guo,Honghong Chang,Yingxiong Wang,Yan Qiao
Lactic acid (2-hydroxypropionic acid, LA) can be produced from abundant and renewable biomass resources via chemocatalytic processes, which exhibited high potential for meeting its tremendous demand in a wide range of applications. The basic Ba(OH) 2 could efficiently catalyze the conversion of d -glucose into LA. In the present study, by utilizing various NMR techniques including, chemical shift titration, 2D diffusion ordered spectroscopy (DOSY) analysis, heteronuclear singular quantum correlation ( 1 H– 13 C HSQC), the binding sites and catalytic interaction between d -glucose and catalyst were clarified. The experimental results by NMR and electrospray ionization mass spectrometry (ESI–MS) were used to explore the reaction mechanism. Experimental results disclosed that the catalyst, Ba(OH) 2 , may exist in the form of [Ba(OH)(H 2 O) 2 ] + under the reaction condition. The OH – of [Ba(OH)(H 2 O) 2 ] + is attached to the C5-O of d -glucose, and Ba 2+ is connected to the C1-O of d -glucose. Herein, we suppose that Ba(OH) 2 and d -glucose interact and form a complex, d -glucopyranose-[Ba(OH)(D 2 O) 2 ] + . Furthermore, when the reaction was performed in D 2 O, we were surprised to find that LA underwent varying degrees of deuteration. The reaction mechanism, binding sites and deuteration of the product have shed light on the efficient conversion of biomass to LA.
研究领域:
生物化学
·
催化化学
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产品关联
DOI
高影响因子
Catalysis Science & Technology
·
2020
·Vol. 10
·期 6
·1609-1618
·
IF 5.721
Chao Zhang,Jiandong Liu,Xingliang Liu,Shiai Xu
Two novel covalent bond photocatalysts, namely, g-C3N4NSs/TC1 and g-C3N4NSs/TC2 were prepared for the first time via the generation of an amide covalent bond between g-C3N4 nanosheets (g-C3N4NSs) and acyl chloride synthesized from triphenylamine-type dyes, 2-cyano-3-(4-(diphenylamino)phenyl)acrylic acid (TC1) or 3,3′-(4,4′-(phenylazanediyl)bis(4,1-phenylene))bis(2-cy-anoacrylic acid) (TC2). The experimental results indicated that triphenylamine D–π–A-type organic dye moieties covalently bonded to g-C3N4 successfully and the spectral response regions of both g-C3N4NSs/TC1 and g-C3N4NSs/TC2 could be extended from ∼460 nm to more than 600 nm. Due to the introduced amide covalent bond, the constructed g-C3N4NSs/TC1 photocatalyst showed remarkably enhanced H2 evolution activity with about 73 555.8 μmol h−1 g−1 and exhibited high apparent quantum yields (AQY) of 35.2%, 27.1%, 16.9% and 5.4% at 420 nm, 500 nm, 520 nm and 600 nm monochromatic light, respectively. Compared with the results for pure g-C3N4 NSs, about 100 times higher H2 production rate and 978 times higher AQY value were obtained for g-C3N4NSs/TC1 with the amide covalent bond under visible light irradiation. Compared to g-C3N4NSs/TC1, g-C3N4NSs/TC2 exhibited slightly lower H2 evolution activity with about 70 986.8 μmol h−1 g−1. Combining the experimental and density functional theory (DFT) calculation results, a possible mechanism can be conjectured that the electronic and geometric structures of g-C3N4NSs/TC1 are more conducive to the charge separation and the unidirectionality of electron transport in the excited state than those of g-C3N4NSs/TC2. The research reveals that this introduction of simple triphenylamine-type dyes into the surface of g-C3N4via covalent links results in a strong covalent interaction between functionalized moieties and g-C3N4 to synergistically improve the electronic and optical properties and photocatalytic performance. The present work highlights the potential use of organic dye covalent-functionalized g-C3N4via post modification as a novel photocatalyst in applications such as H2 production.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
高影响因子
Life-Basel
·
2024
·Vol. 14
·期 6
·713
·
IF 3.2
Leyuan Liu,Lang Yang,Jinxi Yuan,Jie Zhang,Chenhao Liu,Hongxu Zhou,Wei Liu,Guirong Wang
The oriental fruit fly,Bactrocera dorsalis(Hendel), poses a significant threat to the global fruit industry, causing damage to diverse fruits like citrus, mango, and guava. Chemical pesticides have limited effectiveness, and pesticide residues and pesticide resistance are pressing issues. Therefore, it is essential to develop environmentally friendly pest control methods to address this problem. Behavior-modifying chemicals, including male attractants and intersex protein baits, play a critical role in the control ofB. dorsalis. The mature host fruit serves as both an oviposition site and food source under natural conditions, making it a potential attraction source for oriental fruit flies. Orange,Citrus sinensis, is a main host ofB. dorsalis, and commercial orange juice is a common attractant for the egg laying ofB. dorsalis. Although it can both attract and elicit oviposition behaviors inB. dorsalisadults, its active components are still unclear. This study utilized analytical chemistry, behavioral tests, and electrophysiology to identify the active components of commercial orange juice that attractB. dorsalis, with the aim of providing a reference for the development of behavior-modifying chemical-based techniques to controlB. dorsalis.Five compounds with a high abundance were identified via a GC-MS, including D-Limonene, butanoic acid ethyl ester, β-myrcene, linalool, and α-terpineol. Behavioral and electrophysiological experiments uncovered that D-Limonene was the active substance that was the main attractant in the mixture of these five substances, evoking a strong electrophysiological response in adultB. dorsalis. D-Limonene strongly attracts adultB. dorsalisonly when they are sexually mature, and the attraction is not rhythmic. Olfaction plays a leading role in the attraction of D-Limonene to adultB. dorsalis, andOrco−/−mediates the perception of D-Limonene byB. dorsalis. Overall, D-Limonene is one of the key attractant compounds forB. dorsalisin the volatile compounds of commercial orange juice, offering possible support for the development of behavior-modifying chemical-based technology to controlB. dorsalisin the future.
研究领域:
昆虫学
·
化学生态学
·
害虫防治
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）