MOLECULES
·
2019
·Vol. 24
·期 17
·3060
·
IF 3.06
Shuang Gao,Yan-Yan Liu,Jing-Yu Jiang,Ying Fu,Li-Xia Zhao,Chun-Yan Li,Fei Ye
Herbicide safeners selectively protect crops from herbicide injury while maintaining the herbicidal effect on the target weed. To some extent, the detoxification of herbicides is related to the effect of herbicide safeners on the level and activity of herbicide target enzymes. In this work, the expression of the detoxifying enzyme glutathione S-transferase (GST) and antioxidant enzyme activities in maize seedlings were studied in the presence of three potential herbicide safeners: 3-dichloroacetyl oxazolidine and its two optical isomers. Further, the protective effect of chiral herbicide safeners on detoxifying chlorsulfuron in maize was evaluated. All safeners increased the expression levels of herbicide detoxifying enzymes, including GST, catalase (CAT), and peroxidase (POD) to reduce sulfonylurea herbicide phytotoxicity in maize seedlings. Our results indicate that theR-isomer of 3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine can induce glutathione (GSH) production, GST activity, and the ability of GST to react with the substrate 1-chloro-2,4-dinitrobenzene (CDNB) in maize, meaning that theR-isomer can protect maize from damage by chlorsulfuron. Information about antioxidative enzyme activity was obtained to determine the role of chiral safeners in overcoming the oxidative stress in maize attributed to herbicides. The interaction of safeners and active target sites of acetolactate synthase (ALS) was demonstrated by molecular docking modeling, which indicated that both isomers could form a good interaction with ALS. Our findings suggest that the detoxification mechanism of chiral safeners might involve the induction of the activity of herbicide detoxifying enzymes as well as the completion of the target active site between the safener and chlorsulfuron.Keywords:chiral safener;biological activity;antioxidative enzyme activity;protection mechanism;chlorsulfuron
研究领域:
植物生理学
·
分子生物学
·
农药科学
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DOI
PubMed
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2019
·
IF 3.25
Osman Ahmed Zelekew,Dong-Hau Kuo,Hairus Abdullah
Herein, we report the bimetallic (Sn,Zn)(O,S) oxysulfide nanocatalyst with a facile method. The Sn-based catalyst with the addition of Zn was synthesized with the proportions of 0, 20, 30 and 50% of Zn to Sn precursors for preparation. The catalysts were characterized by XRD , TEM , SEM XPS , and UV–vis instruments. The nanocatalysts were also tested for the detoxification of Cr +6 . The Sn-20 catalyst with 20 M percent of Zn(Ac) 2 ·2H 2 O showed an excellent performance for the induced photocatalytic reduction of Cr +6 under visible light irradiation at room temperature. The complete reduction of Cr +6 was achieved within 80 min by Sn-20 catalyst. However, 85.6, 97, and 94% of Cr +6 reductions were achieved within 80 min under visible light illumination by Sn-0, Sn-30, and Sn-50 catalysts, respectively. Hence, the bimetallic (Sn,Zn)(O,S) oxysulfide nanocatalyst will be a candidate and highly potential material for the detoxification of Cr(VI)-containing polluted water.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 30
·期 15
·42587-42595
·
IF 5.19
Yu Haikuo,Yang Xianmei,Dai Jinghua,Li Yuning,Veeran Sethuraman,Lin Jintian,Shu Benshui
The fall armyworm, Spodoptera frugiperda, has become a worldwide pest and threatens world food production. A previous study indicated that azadirachtin, the most effective botanical insecticide for S. frugiperda , inhibits larval growth of the insect. The effect of azadirachtin on the tissues of the larvae, however, remains to be determined. In this study, the effects of azadirachtin on the structure of fat bodies were analyzed. Comparative transcriptomic analysis was conducted between controls and samples treated with 0.1 μg/g azadirachtin for 7 days to explore potential relevant mechanisms. The expression of 5356 genes was significantly affected after azadirachtin treatment, with 3020 up-regulated and 2336 down-regulated. Among them, 137 encode detoxification enzymes, including 53 P450s , 20 GSTs , 27 CarEs , 16 UGTs , and 12 ABC transporters . Our results indicated that azadirachtin could destroy fat body structure and change the mRNA levels of detoxification-related genes. The up-regulated genes encoding detoxification enzymes might be related to detoxifying azadirachtin. Our results elucidate a preliminary mechanism of azadirachtin detoxification in the fat bodies of S. frugiperda larvae.
研究领域:
昆虫学
·
分子生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Runren Jiang,Guanghua Lu,Tianjian Dang,Min Wang,Jianchao Liu,Zhenhua Yan
Photocatalysis is a promising method for water pollution treatment, but it is challenged due to its low efficiency and insufficient understanding of the toxicity after degradation. In this work, we construct a polyethyleneimine-enhanced BiOBr semiconductor organic framework (PEI/BiOBr SOF) by utilizing PEI's bonding and intercalation effects. PEI/BiOBr SOF enhances the photocatalytic degradation of bisphenol AF (BPAF) at a reaction rate 10 times that of BiOBr. Under the dual effects of intercalation and bonding, PEI/BiOBr SOF can realize extended the light absorption, increase the specific surface area and surface-active sites, inhibit carrier complexation, and promote the production of reactive oxygen species. The toxicity changes of the BPAF were evaluated by combining chemical product analysis and biological toxicity experiments. The mutual validation of the toxicity prediction results of the ECOASR product and the zebrafish toxicity experiment confirmed that the toxicity of BPAF decreased after degradation. Furthermore, the mechanism of photocatalytic degradation and detoxification of BPAF with PEI as the circuit of charge transmission of the BiOBr SOF was proposed.
研究领域:
环境科学
·
材料科学
·
毒理学
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Weijie Liu,Yanting You,Di Sun,Shiwei Wang,Jingrong Zhu,Cong Liu
Effluents loaded with various synthetic dyes are considered as a huge burden to the surrounding ecosystems. Sudan dyes are relatively difficult to decolorize due to its water-insolubility. In the present study, the strain Shewanella putrefaciens CN32 was firstly applied to decolorize Sudan dyes under the anaerobic condition , and the physicochemical parameters on the decolorization were optimized. The results demonstrated that the suitable decolorization condition was temperature 26 °C, initial pH 7.0–8.0 and NaCl concentrations 0–20 g/L. Electron competitive acceptors including nitrite, nitrate, dimethyl sulphoxide and oxygen could cause the significant inhibition to the decolorization of Sudan dyes. Biosurfactant rhamnolipid played a positive role in enhancing the decolorization of Sudan I. The co-culture of S. putrefaciens CN32 and Bacillus circulans BWL1061 is reported for the first time to accelerate the decolorization through improving the synergistic effect of enzymatic degradation and biological reductive effect. The highest decolorization of 90.23% to Sudan I was achieved within 108 h, suggesting that co-culture technique has a good potential in the treatment of dyeing wastewater. Furthermore, the microbial toxicity tests indicated that the toxicity of Sudan I to Escherichia coli BL21 and Bacillus subtilis 168 was obviously decreased after the decolorization.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 10
·9585-9597
·
IF 2.76
Yang Xueting,Zheng Jinzhao,Lu Yongming,Jia Rong
Malachite green (MG), a recalcitrant, carcinogenic, and mutagenic triphenylmethane dye, was decolorized and detoxified using crude manganese peroxidase (MnP) prepared from the white rot fungus Irpex lacteus F17. In this study, the key factors (pH, temperature, MG, Mn 2+ , H 2 O 2 , MnP) in these processes were investigated. Under optimal conditions, 96 % of 200 mg L −1 of MG was decolorized when 66.32 U L −1 of MnP was added for 1 h. The K
m , V
max , and k
cat values were 109.9 μmol L −1 , 152.8 μmol L −1 min −1 , and 44.5 s −1 , respectively. The decolorization of MG by MnP followed first-order reaction kinetics with a kinetic rate constant of 0.0129 h −1 . UV–vis and UPLC analysis revealed degradation of MG. Furthermore, seven different intermediates formed during the MnP treatment of 0.5 h were identified by LC-TOF-MS. These degradation products were generated via two different routes by either N -demethylation of MG or the oxidative cleavage of the C–C double bond in MG. Based on ecotoxicity analyses performed on bacteria and algae, it was confirmed that MG metabolites produced by the MnP-catalyzed system were appreciably less toxic than the parent compound. These studies indicate the potential use of this enzyme system in the clean-up of aquatic and terrestrial environments.
研究领域:
环境科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
BIOCONTROL SCIENCE AND TECHNOLOGY
·
2017
·
IF 0.919
Xiaohua Hu,Yajie Guo,Songqing Wu,Zhaoxia Liu,Tao Fu,Ensi Shao,Carballar-Lejarazú Rebeca,Guohui Zhao,Zhipeng Huang,Ivan Gelbič,Xiong Guan,Shuangquan Zou,Lei Xu,Lingling Zhang
Bacillus thuringiensis var. israelensis (Bti) is highly pathogenic to mosquito larvae and is widely used for mosquito control. Its mosquitocidal activity however is relatively low compared to many chemical insecticides. The detoxification mechanisms in the mosquito, among other things, might neutralize the Bti activity, resulting in resistance or tolerance. We tested whether or not the detoxification mechanisms against chemical insecticides might also operate against Bti, rendering it less effective. We targeted four enzymes in Aedes aegypti larvae involved in detoxification with inhibitors that have been used in resistance studies in chemical insecticides and assayed their effects on Bti toxicity. Results revealed that phenylmethanesulphonyl fluoride (PMSF), diethyl maleate, phenobarbital (PB), and piperonyl butoxide (PBO) altered Bti toxicity to various degrees. PMSF is a serine protease inhibitor that prevents Bti digestion and improves Bti activity. PB that induces several detoxifying enzymes had two different effects depending on the method of treatment. Mortality was higher when treatment with PB was discontinuous (149%) whereas with continuous treatment it was lower (101%). PBO, a typical cytochrome P450 inhibitor, increased Bti effect (159%). The combination of discontinuous pretreatment of larvae with PB followed by PBO had a synergistic effect and showed increased activity (146%). It appears that the mechanism for Bti resistance in mosquitoes is similar to that of chemical insecticides. Our studies indicate that we may be able to increase Bti activity by inhibiting some of the detoxification systems as active as broad spectrum chemical insecticides.
研究领域:
昆虫学
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毒理学
·
杀虫剂抗性
·
蚊虫控制
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产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2021
·
IF 9.297
Guotao Mao,Fangyuan Wang,Jie Wang,Pengyu Chen,Xiaodong Zhang,Hongsen Zhang,Zhimin Wang,Andong Song
Laccases are green biocatalysts that are advantageous in the bioremediation of the triphenylmethane dye malachite green (MG). Here, Cblac from Caldicellulosiruptor bescii was characterized to be a novel DUF152 laccase. Cblac mutant Mut8 was designed with enhanced thermostability, which was demonstrated by the Thermofluor assay, circular dichroism spectrum, and structural analyses. MG bioremediation by Mut8 at high temperatures was performed and examined for its potential to degrade and detoxify the high-temperature MG effluent. Approximately 100% of 100 mg/L of MG was degraded by 40 U/L of Mut8 within 2 h at 75 °C. Mut8 initiated the degradation via oxidative cleavage of the central C–C bond and N-demethylation of MG, and the intermediates were further demethylated, hydroxylated and oxidized. Moreover, the systematic toxicity assessment of the MG degradation products using bacteria, Zea mays , and zebrafish demonstrated that Mut8 thoroughly eliminated MG toxicity. Taken together, Mut8 could effectively decolorize and detoxify MG at high temperatures, showing great promise as an environmental friendly and effective approach to remediate MG effluent immediately released from the dyeing process, thus avoiding the cooling process and lowering the cost simultaneously.
研究领域:
环境生物技术
·
生物催化
·
生物修复
·
酶工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Runren Jiang,Guanghua Lu,Tianjian Dang,Min Wang,Jianchao Liu,Zhenhua Yan,Haijiao Xie
The use of photocatalysis to remove pharmaceuticals and personal care products (PPCPs) is emerging as a promising method, but few studies have investigated the simultaneous degradation of multiple PPCPs. Here, we constructed a hydrogen-bonded bridged MIL-101(Cr)–NH 2 /BiOCl-OH (MCN/BOCH) system to degrade sulfamethoxazole and ethyl p-aminobenzoate alone or in combination. The MCN/BOCH system effectively removed two PPCPs under visible light and exerted a synergistic effect on degrading the combined pollutants. Meanwhile, the degradation products of the mixture changed significantly compared with that of a single pollutant, and the toxicity of the composite pollutants was significantly reduced through the combined effect of synergistic degradation. The bridging of hydrogen bonds effectively accelerated charge transfer at the heterojunction interface under visible light. Moreover, the separated redox side of the MCN/BOCH heterojunction system and the electron donor and acceptor effect of the two PPCPs significantly enhanced the synergistic photocatalytic degradation performance. This work might be useful for controllable designs of hydrogen-bonded photocatalysts for the treatment of complex PPCP pollution.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Shengli Zhang,Wei Li,Menglin Li,Tong Lin,Kai Su,Hongwei Yang,Junmin Chen
In the present study, a novel PEI-modified Juncus effuses (PEI-JE) adsorbent was prepared based on the natural 3D network structure of JE via a simple and environmental-friendly method with the help of the ethanol/aqueous solution. SEM, EDS, ATR-FTIR and XPS were performed to reveal the physical and chemical structure of the PEI-JE. The Cr(VI) removal by the PEI-JE was investigated by bath adsorption experiments. Results showed that the developed PEI-JE exhibited the same 3D network structure as the JE, which not only was easy to separate from aqueous solution but also can expose more active sites and facilitate mass transfer. The optimal pH for Cr(VI) adsorption on the PEI-JE was 3, and adsorption equilibrium time was related to initial Cr(VI) concentrations. The adsorption kinetic obeyed pseudo-second-order kinetic model, and the adsorption isotherm showed the best fit with Freundlich model. The calculated maximum adsorption capacity was 268.93 mg/g at 25℃. Coexisting ions including Na + , K + , Al 3+ , H 2 PO 4 − , Cl − , NO 3 – had no remarkable effect on Cr(VI) adsorption, but the presence of SO 4 2− , HCO 3 – and CO 3 2– caused the efficiency losses of 10.9%, 41% and 66%, respectively. After 10 cycles of adsorption/desorption, Cr(VI) removal rate on the PEI-JE almost kept unchanged, demonstrating that the PEI-JE possessed good reusability. Mechanism study suggested that Cr(VI) removal was involved in a three-step reaction, namely electrostatic adsorption, redox and complexation. Film diffusion was the rate-limiting step and chemical adsorption was the main mechanism. The Cr(VI) adsorbed on the PEI-JE surface was completely reduced to Cr(III), demonstrating that PEI-JE is useful for Cr (VI) removal and detoxification.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Yulin Zhao,Haoyan Huang,Ning Lv,Chunyan Huang,Huili Chen,Han Xing,Chaorui Guo,Ning Li,Di Zhao,Xijing Chen,Yongjie Zhang
Genipin (GP), the reactive metabolite of geniposide (GE), is responsible for GE-induced hepatotoxicity. As a potential detoxification pathway, the inactivation of GP by glutathione S-transferases (GSTs) has not yet been characterized. In this study, the thiol-GSH conjugates of GP, M532-1 and M532-2 were first identified and the catalytic activities of GSTs were investigated both in vitro and in vivo. GSTA1-1 and GSTA4-4 showed high activity in the formation of both thiol-GSH conjugates, whereas GSTA4-4 specifically catalyzed M532-2 formation in vitro. The active GST isoforms protect against alkylation of N-acetylcysteine (NAC), a classic model nucleophile. GST inhibition attenuated M532-1 formation in rat bile, confirming the in vivo catalytic role of GSTs. In conclusion, this study demonstrated the inactivation of GP by GSTs and implied that interindividual variability of GSTs may be a risk factor for susceptibility to GE-induced hepatotoxicity.
研究领域:
药理学
·
毒理学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Acta Pharmaceutica Sinica B
·
2023
·
IF 14.5
Zinan Zhang,Wenbin Cao,Huanchun Xing,Shuai Guo,Lijuan Huang,Lin Wang,Xin Sui,Kui Lu,Yuan Luo,Yongan Wang,Jun Yang
In the treatment of central nervous system disease, the blood–brain barrier (BBB) is a major obstruction to drug delivery that must be overcome. In this study, we propose a brain-targeted delivery strategy based on selective opening of the BBB. This strategy allows some simple bare nanoparticles to enter the brain when mixed with special opening material; however, the BBB still maintains the ability to completely block molecules from passing through. Based on the screening of BBB opening and matrix delivery materials, we determined that phospholipase A2-catalyzed 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphoserine liposomes can efficiently carry drugs into the brain immediately. At an effective dose, this delivery system is safe, especially with its effect on the BBB being reversible. This mix & act delivery system has a simple structure and rapid preparation, making it a strong potential candidate for drug delivery across the BBB.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）