SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Yanan Zhang,Dajun Hou,Xueli Yu
A ratiometric fluorescent pH sensor was facilely constructed by covalent modification of amino-terminated silicon nanodots (SiND) with pH-sensitive fluorescein isothiocyanate (FITC). After optimization, the SiND-FITC(40:1) material with a SiND:FITC initial mass ratio of 40:1 was selected for the sensing of hydrogen ions. It was observed that the material inherits the unique features of SiND and FITC, and there is significant improvement of SiND acid−base stability, which is a favorable factor in terms of providing fluorescence reference signal. The SiND-FITC(40:1) material displays not only high pH sensitivity, but also good stability and anti-interference ability, and the response process is highly reversible. Deploying the SiND-FITC(40:1) material, we have made available a simple, sensitive, and precise approach for pH sensing. In aqueous solutions, the I 517 /I 466 fluorescence intensity ratio of SiND-FITC(40:1) increases linearly in the pH range of 5.40–7.76. This dual emission nanosensor was successfully applied for pH sensing and cellular fluorescence imaging.
研究领域:
纳米技术
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材料科学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Chao Chen,Yong Yu,Xiaoli Wang,Ping Shi,Yibing Wang,Ping Wang
Podophyllotoxin (PPT) offers a broad-spectrum of anticancer activities, but little has been reported for its controlled release. This work shows that by manipulating molecular interactions between PPT and Chitosan, efficient nanoscale capsulation of PPT can be realized. The drug encapsulation efficiency is as high as 52%, with a final particle drug loading in the order of 10% (wt/wt). It further demonstrates that changes in pH can also significantly affect the rate of drug release from the Chitosan nanoparticles. Upon contact with cancer cells, chitosan nanoparticles enable efficient internalization and drug release. In vitro evaluations with HepG-2 and MCF-7 cells indicate that the chitosan nanoparticle carriers can improve drug efficacy in comparison to free PPT, most likely by regulating the intrinsic apoptotic signaling pathway to induce apoptosis. Overall, PPT chitosan nanoparticles promise a safe and efficient drug delivery system for PPT.
研究领域:
药理学
·
生物材料
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2018
·
IF 1.911
Nahla Rahoui,Bo Jiang,Nadia Taloub,Mohammad Hegazy,Yu Dong Huang
The accuracy of spatiotemporal control cargo delivery and release are primordial to enhance the therapeutic efficiency and decrease the undesirable effects, in this context a novel prodrug were developed based on biocompatible polyvinyl alcohol (PVA) substrate. PVA was conjugated to doxorubicin (PVA-DOX) via an acid-labile hydrazone linkage. PVA was first functionalized with acidic groups, then reacted with hydrazine hydrate to form an amide bond. The amine group of PVA hydrazide was linked to carbonyl group (C = O) of DOX to form a pH sensitive hydrazone bond. The molecular structure of the PVA-DOX was confirmed by FTIR, XPS, and 1H-NMR analysis methods. The degree of grafting were evaluated by TGA and confirmed by XPS, which reveals the successful bond attachment of DOX to PVA. Our findings confirm pH dependent DOX release from PVA-DOX prodrug with faster release rate in acidic environment (pH 5.0, pH 6.0) and slower release rate in neutral pH environment (pH 7.4). Compared to the primary DOX, our synthesized PVA-DOX conjugates could exhibit a promising therapeutic effect, high biocompatibility and zero premature release. The results prove the successful synthesis of PVA-DOX conjugates with high efficiency.
研究领域:
药剂学
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生物材料学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 5
·2070-2079
·
IF 3.639
Hang Liu,Junbing Zhang,Yong Xiong,Shengfeng Peng,David Julian McClements,Liqiang Zou,Ruihong Liang,Wei Liu
BACKGROUND Norbixin, a carotenoid extracted from annatto seeds, is widely utilized as a natural pigment in foods, cosmetics and medicines. Its water solubility is relatively high under neutral or alkaline conditions but low under acidic conditions, which limits its application in some food products. RESULTS This problem was overcome by utilizing liposomes to encapsulate the carotenoids so that they could be easily dispersed within acidic solutions. The norbixin was loaded into the liposomes using the pH-driven method. Liposomes were produced by passing aqueous phospholipid dispersions through a microfluidizer under high pressure. Norbixin was then added to the liposome dispersions at pH 7.0 and then driven into the hydrophobic domains of the phospholipid bilayers by acidifying the system. Measurements of the encapsulation efficiency showed that the norbixin was successfully loaded into the liposomes using the pH-driven method. X-ray diffraction analysis showed that the norbixin was in an amorphous state after incorporation into the liposomes. Encapsulation of norbixin within the liposomes was also shown to increase its water dispersibility and chemical stability under acidic pH conditions. CONCLUSION The pH-driven method therefore provides a useful means of increasing the application of this bioactive carotenoid within functional foods and other products. © 2021 Society of Chemical Industry.
研究领域:
食品科学
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生物技术
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Xiaoxiao Hu,Qiang Zhang,Xiaomei Dai,Junyong Sun,Feng Gao
The pH environment in cancer cells has been demonstrated to display vital influences on the therapeutic effect of photodynamic therapy (PDT). It is very interesting to develop pH-responsive probes for simultaneous pH sensing and dynamics monitoring of the effects of PDT, and therefore assessing the correlation between them. In this study, a multifunctional fluorescence probe, dual-emission carbonized polymer dot (CPD) in blue and red regions, which uses ethylene imine polymer (PEI) and 4,4′,4″,4‴-(porphine-5, 10, 15, 20-tetrayl) tetrakis (benzoic acid) (TCPP) as precursors through a one-step hydrothermal amide reaction, has been designed for ratiometric pH sensing, generating pH-dependent 1O2 for PDT of cancer cells, and investigating the dynamics effects of PDT through pH-guided imaging. The prepared CPDs were successfully used for ratiometric pH response, pH-dependent generation of 1O2, and dynamics monitoring PDT in HeLa cells. This study may provide an alternative strategy to prepare CPD-based theranostic integrated nanoprobes for PDT through the rational design of precursors.
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Haiqing Lu,Tongda Lei,Li Zhang,Shiyang Chen,Rouxi Chen,Xipeng Li,Yongheng Wang,Jian Zhu,Mingyi Chen,Kai Zhang,Qingqi Yang,Sen He,Jie Fan
A low-cost flexible polyaniline@carbon nanofiber membrane (PANI@CNM) electrode was fabricated by chemical in situ polymerization of aniline monomer for pH detection. The preparation condition, morphology, chemical structure and the electrochemical properties of the PANI@CNM electrode were investigated. The result showed that PANI@CNM electrode with high sensitivity of 76.2 mV/pH was prepared with 0.3 M aniline solution and hydrochloric acid as the doping acid at 0℃ for 6 h. FTIR and Raman analysis suggested that the pH sensibility of PANI is attributed to the super-Nernstian behavior of the PANI in the emerald imine form. The PANI@CNM working electrode performs a stable electrochemical property without interference by the common metal iron and biochemical, and has an outstanding reversible pH response in the pH range of 6.32–8.34 without interference of deformation. It also maintained a stable sensing performance in the temperature range of 25-40℃ for long time use and presented excellent biocompatibility. Generally, the PANI@CNM working electrode exhibits great potential in flexible biomedical and wearable pH detection application.
研究领域:
材料科学
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生物医学工程
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2025
·Vol. 17
·期 5
·663
·
IF 4.9
Longzhou Zhang,Baoyuan Ma,Zhiguang Xu,Yan Zhao
Wearable, non-invasive sweat sensors capable of continuously monitoring the pH of sweat, which is a key indicator related to metabolism and homeostasis level, are highly desirable for personal health management. However, ensuring the stability and accuracy of these sensors can be challenging when the body is in motion. In this work, we prepared a stretchable nanofibrous membrane-based electrochemical pH-sensing electrode by embedding carbon nanotubes (MWCNT) and silver nanowires (AgNWs) into an elastic electrospun nanofibrous membrane, followed by polyaniline electrodeposition. The as-prepared pH-sensing electrode showed a high sensitivity of 82.53 mV/pH and high accuracy in ionic solutions with pH ranging from 3 to 7. Notably, the electrode maintained stable sensing performance under deformations, including torsion, bending, and tensile strains up to 30%. Even after 1000 cycles of stretching at a 30% tensile strain, the detection sensitivity remained above 70 mV/pH, indicating its potential application as a wearable electrochemical sensor for monitoring sweat pH in personal health management.
研究领域:
生物医学工程
·
材料科学
·
健康监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Wei Liu,Meipeng Huang,Jianwei Liang,Xudong Luo,Gao Yang,Di An,Sai Wei,Zhipeng Xie
In this work, we present a preparation strategy for pH-responsive hierarchically porous bioinspired Al 2 O 3 ceramic membrane (pH-HPBA-CM). The pore size of the pH-HPBA-CM could be adjusted by altering the pH value. With the increase of the pH value, the porosity gradually decreases and the compressive strength of the pH-HPBA-CM is also affected by the pH value, which presents an increasing trend with the increase of the pH value. The variation of compressive stress versus displacement of the pH-HPBA-CMs (stress-strain relationship) prepared at different pH values was discussed. A mechanical simulation experiment was carried out to better analyze the reason why the honeycomb-like structure of the pH-HPBA-CM has excellent specific strength . In the simulation process, the small pore model (15 μm) can be regarded as part of the large pore model (35 μm). The simulation of the total deformation, equivalent stress, and equivalent elastic strain were obtained by setting the load pressure of 100 MPa. At this load pressure, the maximum deformation of the entire ceramic membrane is far lower than the theoretical limit value of its simulation, and all of them are distributed at the junction (connectional pores). This work provides a new insight into the effective preparation of pH-responsive hierarchically porous bioinspired ceramic membranes with high porosity, excellent mechanical performance, and tunable pore size.
研究领域:
材料科学
·
仿生学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Hao Feng,Xun Zhu,Rong Chen,Qiang Liao,Dingding Ye,Biao Zhang,Jian Liu,Ming Liu,Gang Chen,Kun Wang
In this study, the pH-response of the zwitterionic polydopamine layer to the palladium deposition in a capillary microchannel and the resultant catalytic performance for the nitrobenzene hydrogenation were explored. Experimental results showed that the polydoapmine layer exhibited the switchable surface charge from positive to negative with increasing the pH value. Under low K 2 PdCl 4 concentration, the intrinsic low pH not only allowed most palladium to be deposited at the inlet region of the microchannel, but also provided more accessible sites for the anion PdCl 4 2− deposition. Such property resulted in less palladium deposited at the outlet region because of the limited supply of the precursor and poor mass transfer, while an excellent size distribution of the deposited palladium nanoparticles at both the inlet and outlet of the microreactor was obtained. With the increase of the K 2 PdCl 4 concentration, the increased pH value of the precursor solution aggravated the dispersity of the deposited palladium species and led to large palladium particles at the upstream of the microreactor. The gradually lowered pH value of the precursor solution along the microchannel and the large concentration gradient could directly intensify the anion PdCl 4 2− deposition. Thus, an inverse palladium distribution between the inlet and outlet regions was observed due to the shift of surface potential from negative to positive along the microchannel. Such feature influenced both the catalytic activity and the durability for the nitrobenzene hydrogenation. The obtained results in this work are beneficial for not only the controllable metal catalyst preparation but also the improvement in the economy utilization of catalyst.
研究领域:
化学工程
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
Materials Research Express
·
2021
·Vol. 8
·期 6
·065306
·
IF 1.62
Shangjie Jiang,Shisheng Zhou
In this paper, a simple method for preparing high stability superhydrophobic paper with pH-induced wettability transition was proposed. Firstly, the pH-responsive monomer 2- (dimethylamino) ethyl methacrylate (DMAEMA), the silicon-containing crosslinking monomer 3-trimethoxysilyl propyl methacrylate (TSPM) and the fluorine-containing monomer hexafluorobutyl methacrylate (HFMA) were polymerized to prepare the pH-responsive polymer PHFMA-PTSPM-PDMAEMA. Afterwards, the amino-modified SiO2 was grafted onto the polymer to provide roughness and then coated on the paper to prepare the superhydrophobic paper with pH-responsive properties. Further research found that the modified paper prepared by this method not only has strong stability and transparency, but also can realize the reversible regulation of superhydrophobic & lipophilic and super-oleophobic & hydrophilic properties under different pH-induction, and it has important application value in the field of oil-water separation in industrial applications.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Lixia Guo,Xueyun Zhang,Danning Wen,Lei Ding,Yan Niu,Lihong Li,Wen Liu,Haipeng Diao,Liheng Feng
Accurate measurement of pH is of great significance in the research of biology and medicine sciences. Cell is the basic unit of living organism and the proper pH of cytoplasm and organelles such as lysosome is the precondition to maintain its function. Alteration of pH may impair the normal process of cells and lead to occurrence of diseases. Sensitively sensing the pH of cytoplasm and organelles is of great value for biomedical research. Due to the complexity of biological microenvironment , it is still challenging to simultaneously detect the pH changes of cytoplasm and lysosome. Here, we reported a ratiometric pH probe with NIR fluorescence termed as PCHC, which realized sensitive cytoplasmic and lysosome pH detection. The probe was prepared by connecting coumarin with dicyanmethylene-4H-pyran via amidation reaction, and displays large Stoke-shift, good biosafety and pH selectivity. After entering the cell, the probe shows blue in cytoplasm and NIR fluorescence in lysosome due to respective pH. The ratio of blue and NIR fluorescence signal can be served as indicator to monitor pH change of cytoplasm and lysosome simultaneously, which has potential application for investigation of pH alteration related diseases.
研究领域:
生物医学
·
细胞生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2015
·
IF 3.368
Wenlong Xu,Huizhong Liu,Aixin Song,Jingcheng Hao
Bilayers at high pH in the fatty acid systems of palmitic acid/KOH/H 2 O, palmitic acid/CsOH/H 2 O, stearic acid/KOH/H 2 O and stearic acid/CsOH/H 2 O can form spontaneously (Xu et al., 2014, 2015). In this work, the bilayers can still be observed at 25 °C with an increase of the concentration of fatty acids. We found that wormlike micelles can also be prepared in the fatty acid soap systems at high pH, even though the temperature was increased to be 50 °C. The viscoelasticity , apparent viscosity, yield stress of the bilayers were determined by the rheological measurements. Wormlike micelles were identified by cryogenic transmission electron microscopy (cryo-TEM) and emphasized by the rheological characterizations, which are in accordance with the Maxwell fluids with good fit of Cole–Cole plots. The phase transition temperature was determined by differential scanning calorimetry (DSC) and the transition process was recorded. The regulating role of counterions of fatty acids were discussed by (CH 3 ) 4 N + , (C 2 H 5 ) 4 N + , (C 3 H 7 ) 4 N + , and (C 4 H 9 ) 4 N + as comparison, concluding that counterions with appropriate hydrated radius were the vital factor in the formation wormlike micelles.
研究领域:
胶体化学
·
表面活性剂科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）