Journal of Pharmaceutical Analysis
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2019
·
IF 4.44
Satar Tursynbolat,Yrysgul Bakytkarim,Jianzhi Huang,Lishi Wang
The simultaneous electrochemical determination of myricetin and rutin remains a challenge due to their indistinguishable potentials. To solve this problem, we constructed a ternary platinum nanoparticle, reduced graphene oxide, multi-walled carbon nanotubes (Pt@r-GO@MWCNTs) nanocomposite via a facile one-pot synthetic method. Under the optimized conditions, the ternary Pt@r-GO@MWCNTs nanocomposite exhibited good electrocatalytic activity toward myricetin and rutin via solid phase extraction and excellent performance for the simultaneous determination of myricetin and rutin. The oxidation peak current of myricetin was proportional to its concentrations in the range of 0.05–50 μM with a detection limit of 0.01 μM (S/N = 3). The linear range for rutin was 0.05–50 μM with a detection limit of 0.005 μM (S/N = 3). The ternary nanocomposite sensor also exhibited good reproducibility and stability, and was successfully used for the simultaneous determination of myricetin and rutin in real orange juice samples with recoveries ranging between 100.57% and 108.46%.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qing Huang,Chen-dong Ling,Wen-feng Zhuge,Wei Huang,Feng-ping Liu,Cui-zhong Zhang,Jin-yun Peng,Fu-xing Huang
An efficient electrochemical sensor for rutin determination based on CeO 2 /NiCo 2 S 4 acanthosphere-like microspheres and multi-walled carbon nanotubes (MWCNTs) modified electrode was proposed. In this work, we design an acanthosphere-like CeO 2 /NiCo 2 S 4 heterojunction by an “in situ growth” method of hydrothermal process. Then MWCNTs were introduced into heterostructured microspheres to form composites of CeO 2 /NiCo 2 S 4 /MWCNTs. The morphology and elemental properties of the electrochemical materials were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The redox mechanism of rutin on the working electrode surface and the electrochemical properties were investigated by cyclic voltammetry (CV), chrono coulometry (CC) and electrochemical impedance spectroscopy (EIS). The results show that the CeO 2 /NiCo 2 S 4 /MWCNTs modified glassy carbon electrode exhibited excellent electric conductivity and electrocatalytic activity toward rutin, and increased effective working area. Under the optimized condition, the fabricated sensor expressed a good linear response for rutin analysis ranging from 0.5 to 65.4 μM with a low detectable limit of 4.37 × 10 -2 μM (S/N = 3). The electrochemical sensor shows good reproducibility and reliable stability, high sensitivity and selectivity, and has been successfully applied for rutin determination in orange juice and human urine samples.
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Fermentation-Basel
·
2024
·Vol. 10
·期 2
·87
·
IF 3.3
Wenjiao Xue,Chen Liu,Yao Liu,Hao Ding,Chao An,Shizhe Zhang,Saijian Ma,Qiwen Zhang
To assess the probiotic potential of strain 68-1 with rutin conversion capabilities, isolated from Chinese traditional Jiangshui, a complete genomic analysis and in vitro tests were conducted. The Oxford Nanopore Technologies (ONT, Oxford, UK)–Illumina (San Diego, CA, USA) hybrid sequencing platform was used for whole genome sequencing and the results showed that strain 68-1 had a chromosome sequence of 3,482,151 bp, with 46.53% GC content and five plasmids with a sequence length ranging from 2009 bp to 48,711 bp. Strain 68-1 was identified asLactiplantibacillus pentosusbased on the whole genome sequence. A total of 133 genes encoding for carbohydrate-active enzymes (CAZymes) were identified and genes that may be involved in rutin conversion were found in theL. pentosus68-1 genome.L. pentosus68-1 showed excellent tolerance to gastrointestinal juice and adhesion properties, and corresponding genes were identified. In addition,L. pentosus68-1 exhibited strong antibacterial and antifungal activity, where organic acids may play a crucial role in its antagonistic ability. Moreover, the gene cluster for plantaricin_EF production was detected. No high virulence factor was found in theL. pentosus68-1 genome and no hemolytic effect was observed. In addition,L. pentosus68-1 showed resistance to ampicillin, gentamycin, and kanamycin, and the genomic analysis indicated that horizontal ARG transfer should not be possible. The results show thatL. pentosus68-1 could be developed as a novel probiotic candidate to improve rutin bioavailability in the food and feed industry.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Weisong Wu,Limei Liu,Yingwei Zhu,Jingbin Ni,Jian Lu,Xiaoli Wang,Li Ma,Yanjun Jiang
In patients suffering from inflammatory bowel diseases (IBDs), the immune system is disrupted and the intestinal barrier function is compromised. Here, six zinc-flavonoid particles were produced by one-step reaction via changing flavonoids (myricetin, quercetin, and rutin) and solvent (water and ethanol), and then their cytocompatibility and ability to scavenge H2O2, free radicals, and LPS-induced ROS were compared. Zinc-rutin particles (W-ZnRT) composed of rutin (78.92 wt %), Na12[ZnPO4]12·12H2O (6.76 wt %), and crystal water were screened out because W-ZnRT exhibited 80.8 ± 15% cell viability against RAW264.7, could rapidly scavenge 78.1 ± 1% of H2O2 and 71.6 ± 2% of DPPH within 30 min, and reduced LPS-increased intracellular ROS to normal levels. In addition, the therapeutic effects of rutin and W-ZnRT were also compared in dextran sulfate sodium (DSS)-induced acute and chronic colitis in mice. W-ZnRT was superior to rutin alone in chronic colitis (n = 9), although they were equally effective in acute colitis (n = 7). Compared to rutin, 11 oral doses of W-ZnRT (40 mg kg–1) significantly improved intestinal permeability (p = 0.0299) and colon length (p = 0.0025), reduced intestinal proinflammatory factors (IL-6, IL-1β, and TNF-α), and upregulated tight junction proteins to maintain intestinal barrier function. Taken together, these results identified W-ZnRT as an efficient and safe therapeutic strategy for IBD.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-8
·
IF 5.705
Wang Zonghua,Yu Gege,Xia Jianfei,Zhang Feifei,Liu Qingyun
The authors demonstrate the exploitation of reduced graphene oxide (RGO) as a template for immobilizing zeolitic imidazolate framework-8 (ZIF-8) crystals loaded with the electrochemical probe Methylene Blue (MB). The framework was deposited on the surface of RGO in a one-pot process. Transmission electron microscopy, scanning electron microscopy and X-ray diffraction were employed to characterize the nanocomposite. The electrochemical behavior of rutin at a glassy carbon electrode (GCE) modified with the nanocomposite was investigated by cyclic voltammetry and differential pulse voltammetry. The modified GCE displays high electrocatalytic activity toward rutin oxidation at a relatively low working potential (0.4 V vs. Ag/AgCl). Under the optimal conditions, the sensor has an amperometric response that is linear in the 0.1 to 100 μM rutin concentration range, with a 20 nM detection limit (at an S/N ratio of 3). The method was successfully applied to the determination of rutin in tablets and urine samples.
The zeolitic imidazolate framework ZIF-8 was loaded with Methylene Blue and deposited on the surface of reduced graphene oxide. A glassy carbon electrode was modified with the nanocomposite and then used for the determination of rutin with a 20 nM detection limit and a linear range from 0.1 to 100 μM.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Wen Lv,Mengyuan Zhang,Yange Chen,Yilin Wang,Xuanbo Liu,Yibin Zhou,Yongquan Wang
This study investigated how the interaction between wheat glutenin (WG) and either hydroxypropyltrimethyl ammonium chloride chitosan (HACC) or carboxymethyl cellulose (CMC) regulates the stabilization of HIPE. The potential of these HIPE was then evaluated as a base material for 3D-printed dysphagia foods and a vehicle for rutin delivery. The hydrogen bonds and electrostatic interactions between WG and HACC or CMC was verified using FTIR and intrinsic fluorescence, while molecular docking elucidated hydrogen bonds and hydrophobic interactions. These non-covalent interactions inhibit excessive aggregation, resulting in the formation of smaller complex particles. The interfacial layer thickness quadrupled compared to WG alone. The WG-CMC complexes (WC) were 62–142 nm thicker than the WG-HACC complexes (WH). This aligns with the wettability of WC, which had a three-phase contact angle of 91°. HIPE with an 80% internal oil phase was stabilized using WC and WH. The droplet sizes of the WC-stabilized HIPE (WCE) were 4–35 μm smaller than those of the WH-stabilized HIPE (WHE). All HIPE displayed elastic characteristics, showing higher G′ compared to G′′. The HIPE stabilized by WC at pH 7 (WCE7) exhibited the highest G′, G′′, and viscosity, consistent with its interconnected network microstructure. This structural configuration resulted in the superior stability and high-accuracy 3D printing of WCE7. All the product was classified as a level 4 dysphagia food under the International Dysphagia Diet Standardization Initiative (IDDSI). The HIPE encapsulated 96.7% of the rutin, providing a 32.2% increase in photostability and a bioaccessibility of over 62.7%.
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PHYTOMEDICINE
·
2026
·
IF 11.3
Yeqiu Xu,Junrui Jonathan Hai,Duan Sun,Yihan Yang,Chen Wang,Xinan Zhang,Huanli Xu,Yajin Liao,Xianjun Qu,Bo Han,Weishi Liang,Yong Hai
Background Distractive spinal cord injury (DSCI) is a severe complication of spinal deformity correction surgery, causing early disruption of the blood–spinal cord barrier (BSCB). However, the molecular mechanisms that translate mechanical stress into vascular endothelial dysfunction remain unclear, and effective pharmacological strategies targeting this process are lacking. Purpose This study aimed to investigate the therapeutic effects of sodium rutin (NaR), a water-soluble derivative of rutin, on BSCB disruption following DSCI, and to elucidate the underlying molecular mechanisms. Methods In vivo rat DSCI models and in vitro mechanical distraction models of spinal cord microvascular endothelial cells were employed. The therapeutic effects of oral NaR administration on locomotor function and BSCB permeability in rats following DSCI were systematically evaluated. Mechanistic investigations targeting the SIRT1/β-catenin axis and oxidative stress were conducted utilizing RNA sequencing, interventions with small interfering RNA and SIRT1 inhibitors, alongside standard molecular biology techniques. Results NaR treatment dose-dependently improved locomotor recovery in DSCI rats and significantly reduced BSCB permeability by restoring the expression of tight junction proteins. Mechanical distraction caused a significant downregulation of SIRT1 in endothelial cells. NaR treatment upregulated SIRT1 expression and promoted its nuclear-to-cytoplasmic translocation. This upregulation of SIRT1 was associated with significant inhibition of mitochondrial oxidative stress. Furthermore, oxidative stress was found to promote β-catenin phosphorylation and subsequent degradation via the ubiquitin-proteasome pathway. NaR stabilized β-catenin through SIRT1-mediated antioxidant effects, promoting its nuclear translocation and the downstream expression of tight junction genes. Consequently, by repairing the BSCB, NaR restricted the infiltration of immune cells (CD68 + macrophages/microglia and MPO + neutrophils) into the spinal parenchyma, thereby attenuating secondary neuroinflammation. Conclusion The SIRT1/β-catenin axis may serve as a critical mechanotransduction pathway regulating BSCB integrity. NaR protects the BSCB after DSCI by restoring SIRT1 activity, suppressing oxidative stress, and stabilizing β-catenin signaling. NaR may be a promising pharmacological strategy for the treatment of DSCI. In brief: Hai et al. identify a mechanism by which mechanical distraction disrupts the blood-spinal cord barrier through suppression of SIRT1 and oxidative destabilization of β-catenin. Oral sodium rutin restores barrier integrity, limits inflammation, and thus improves locomotor recovery, highlighting a vascular-targeted therapeutic strategy for distraction-induced spinal cord injury Download: Download high-res image (296KB) Download: Download full-size image
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Feng Liu,Li Chen,Kangling Yin,Taotao Fan,Zongcheng Yan
The development of promising environmentally friendly extraction tools has been one of the hottest topics in green chemistry. Among these, natural deep eutectic solvents (NADESs) as green extraction media have received widespread recognition. In this paper, with the aim of exploring the potential applications of NADESs in the extraction field, water-soluble n -propanol was used to form liquid–liquid biphasic systems with choline chloride/ maltose NADESs. The phase behavior of those systems was designed by adjusting the molar proportion of the hydrogen bond acceptors (HBA): hydrogen bond donors (HBD) and temperature. This allows for the systems tailored to the specific requirements of the target molecule, as shown by their application in the extraction of rutin. Investigation of the distribution behavior of rutin suggested that the partition coefficient and the extraction efficiency of rutin in the bottom phase increase significantly with decreasing temperature and increasing HBA : HBD molar ratio. And with the temperature of 288.15 K, the highest TLL of NADES (ChCl: Mal = 1:2) + NPA + water system achieved higher E% of 94.27 %. The interaction force between NADES and rutin was studied using the Hansen solubility parameter, it turned out that Δ δ i , j of the 2:1 and 1:2 M ratio of NADES was only 8.91, 5.12, which meaning that there may be strong interactions between NADES and rutin. In addition, the hydrogen bond ( Δ δ H ) of the NADES (HBA: HBD = 1:2) was 25.47, which is greater than other interaction force, revealing that the hydrogen bond is the maximum force between rutin and the NADES (HBA: HBD = 1:2). The energy ( Δ G T ), enthalpy ( Δ H T ) and entropy ( Δ S T ) calculated from the partition data of rutin was consistent with the free energy ( Δ G C ) , enthalpy ( Δ H C ) and entropy ( Δ S C ) changes of system, which confirmed that the driving force of the phase separation of the system drives the transfer of rutin to the lower phase. In addition, we used eco-friendly water to regenerate NADES. The recovery efficiency of rutin was explored while NADES was reused in three cycles. This result showed there was no change observed in the extraction capability for rutin in those system. Finally, a larger aggregate of size 1315 nm was observed by dynamic light scattering, which furthermore demonstrated that the hydrogen bonding interaction between NADES (HBA: HBD = 1:2) and rutin is the main driving force tool for enhancing rutin partitioning. This method presents a newly system constructed by NADES and NPA for the green extraction, revealing the feasibility of the system for rutin extraction and facilitating industrial applications.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·Vol. 103
·期 5
·2367-2377
·
IF 4.1
Shun Chen,HuiJuan Liu,JiaQi Zhang,BinBin Zhou,XiaoFang He,Tian Wang,Chao Wang
BACKGROUND This study was conducted to investigate the effect of dietary rutin on the meat quality, antioxidant status and mitochondrial structure and function in the breast muscle of heat-stressed broilers. A total of 192 male broilers were randomly assigned into three groups and treated with normal control (CON), heat stress (34 °C, HS), and HS with 500 mg kg −1 rutin supplementation (HS + Rutin), respectively. RESULTS Dietary rutin significantly reversed HS-induced decrease in body weight, average daily feed intake, average daily gain, and feed efficiency. Rutin supplementation attenuated HS-induced impaired meat quality by decreasing the lightness, drip loss at 24 and 48 h, the peak time of free water ( T 22 ) and the peak area ratio of free water ( P 22 ), and increasing the pH 24h and peak area ratio of immobilized water ( P 21 ). Rutin supplementation promoted superoxide dismutase, glutathione peroxidase activities and total antioxidant capacity, and decreased malondialdehyde levels compared with the HS group. Moreover, rutin attenuated HS-induced mitochondrial damage by increasing the mitochondrial DNA copy number and improving mitochondrial morphology. Dietary rutin significantly increased mitochondrial biogenesis-related mRNA (proliferator-activated γ receptor coactivator-1α [PGC-1α], nuclear respiratory factor 1 [NRF1], and mitochondrial transcription factor A [TFAM]) expression via the AMP-activated protein kinase (AMPK) signaling pathway. HS significantly increased mitophagy-related genes and proteins (Parkin, PTEN-induced putative kinase 1 [PINK1], microtubule associated protein light chain 3-II [LC3-II]) expression, and dietary rutin significantly reversed these alterations. CONCLUSION Dietary rutin attenuated the HS-induced decline in meat quality and antioxidant capacity of broilers, which may be related to inhibition of the AMPK/PINK1–Parkin signaling pathway to attenuate mitochondrial damage. © 2023 Society of Chemical Industry.
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FOOD CHEMISTRY
·
2019
·
IF 5.399
Zhibing Huang,Lijuan Zhang,Yanling Wang,Heng Gao,Xiujiang Li,Xinyu Huang,Ting Huang
The mycotoxin citrinin is often produced during fermentation of Monascus products. We studied the effects of flavonoids on citrinin production by Monascus aurantiacus Li AS3.4384 (MALA) by adding rutin, α-glucosylrutin, or troxerutin to the fermentation medium, in a first-of-its-kind study. Appropriate amounts of rutin, α-glucosylrutin, or troxerutin did not affect normal mycelial growth. Addition of 5.0 g/l of rutin only weakly reduced (29.2%) citrinin production, relative to inhibition by 5 g/l α-glucosylrutin or troxerutin (by 54.7% and 40.6%, respectively). In starch inorganic liquid culture media, addition of 20.0 g/l of troxerutin, followed by fermentation for 12 days, reduced citrinin yield by 75.26%. Addition of 15.0 g/l of troxerutin to low-starch peptone liquid fermentation media reduced citrinin yield by 87.9% after 14 days of fermentation, and addition of 30.0 g/l troxerutin to yeast extract sucrose liquid media for 12 days reduced citrinin yield by 53.7%.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yu-Fan Zhan,Zhuo-Hao Meng,Cheng-Hai Yan,Min Tan,Marriam Khurshid,Yi-Jiangcheng Li,Shao-Jun Zheng,Jun Wang
Enzymatically modified isoquercitrin (EMIQ) is a glyco-chemically modified flavonoid exhibiting notably high biological activity, such as antioxidant, anti-inflammatory and anti-allergic properties. However, the utilization of expensive substrates such as isoquercitrin and cyclodextrin in the conventional approach has hindered the industrial-scale production of EMIQ due to high cost and low yields. Hence, the development of a cost-effective and efficient method is crucial for the biological synthesis of EMIQ. In this study, a natural cascade catalytic reaction system was constructed with α -L-rhamnosidase and amylosucrase using the inexpensive substrates rutin and sucrose. Additionally, a novel approach integrating gradient temperature regulation into biological cascade reactions was implemented. Under the optimal conditions, the rutin conversion reached a remarkable 95.39% at 24 h. Meanwhile, the productivity of quercetin-3- O -tetraglucoside with the best bioavailability reached an impressive 41.69%. This study presents promising prospects for future mass production of EMIQ directly prepared from rutin and sucrose.
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Food & Function
·
2017
·Vol. 8
·期 7
·2512-2522
·
IF 3.247
Qiang Wang,Juan Huang,Caibiao Hu,Nan Xia,Tong Li,Qiang Xia
Literature examples of non-aqueous Pickering emulsions stabilized by fat crystals are very rare. Moreover, the applications of rutin are limited due to its low solubility in both water and oils (less than 0.10 mg g−1 and 0.25 mg g−1, respectively). Thus, herein, we developed an optimum formulation of a non-aqueous self-double-emulsifying delivery system (SDEDS) containing rutin and evaluated its oral bioavailability. The new formulation stabilized by fat crystals (glycerol monostearate, GMS) and nonionic surfactants was prepared via a two-step emulsification process. The presence of a mixture of GMS crystals and nonionic surfactants effectively improves the stability of the emulsions. The non-aqueous SDEDS spontaneously forms oil-in-oil-in-water (O/O/W) double emulsions in the gastrointestinal environment with the inner oil phase mainly containing the active ingredients. It is stable at both 4 °C and 25 °C for 30 days and could enhance the dissolution properties of the active ingredients. Furthermore, the protection of rutin against digestion-mediated precipitation was observed when the formulation contained a high concentration of GMS crystals. The oral absolute bioavailability of rutin obtained from SDEDS (8.62%) is 1.76-fold higher than that of the actives suspension (4.90%). Thus, the non-aqueous SDEDS is an attractive candidate for the encapsulation of water-insoluble and simultaneously oil-insoluble nutrients (such as rutin) and for use in oral delivery applications.
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