LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yanpeng Zhang,Qiudie Liu,Jiahua Wang,Wei Xu,Guozhen Wang,Zhixiong Hu,Chun Hu
The purpose of this study was to fabricate the binary complexes stabilized emulsion delivery systems for β-carotene using sodium caseinate (NaCas) and high methoxy pectin (HMP), low methoxy pectin (LMP) and ι-carrageenan (CA), and to study the stability and digestibility of the complex emulsion systems. Compared to NaCas emulsion, the complex emulsion systems had higher particle size and the absolute value of ζ-potential, and exhibited better physical stability and better protection of β-carotene from thermal degradation. It was demonstrated that the increase in viscosity of the complex emulsions and the improvement of the interfacial viscoelasticity of the NaCas-HMP and NaCas-LMP complexes were conducive to the retention of β-carotene. In vitro digestion experiments showed that the complex emulsions could pH-responsively release β-carotene in the gastrointestinal tract at a release rate of more than 70%, and the bioaccessibility of β-carotene in complex emulsions was up to 72.6%, 76.6% and 80.2% respectively, all of which were related to the interfacial and rheological properties of the complex emulsion. These results illustrate the relationship between the physicochemical properties of the complex emulsions and the stability and digestibility of the emulsion system, which facilitate the design of an emulsion delivery system for bioactive substances.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Nan Ma,Rong Li,Gui-Fang Zhang,Ruo-Han Gao,Dong-Jie Zhang
Quinoa β-glucan (QBG) has shown potential benefits in treating type 2 diabetes mellitus (T2DM); however, comprehensive evaluations of its effects remain limited. This study investigates the impact of QBG—derived from hot water extraction (Q-) and microbial fermentation enrichment (Q+)—on serum glucose levels, lipid profiles, appetite-regulating hormones, fecal short-chain fatty acids (SCFAs), and gut microbiota composition and function in streptozotocin/high-fat diet (STZ/HFD)-induced T2DM mice. The results indicate that QBG treatment significantly reduced fasting blood glucose, insulin levels, triglycerides (TG) and total cholesterol (TC), while concurrently increasing high-density lipoprotein cholesterol (HDL C) levels. Additionally, liver and pancreatic function improved, as evidenced by decreased levels of malondialdehyde (MDA), aspartate transaminase (AST), and alanine transaminase (ALT). SCFA levels were significantly higher in QBG-treated groups compared to MC group. QBG treatment also reduced the abundance of Firmicutes and Patescibacteria, along with the Firmicutes/Bacteroidota ratio, while increasing levels of Bacteroidota and Actinobacteria. These findings suggest that QBG can regulate the dysbiosis of SCFAs production in T2DM mice and may indirectly modulate the secretion of appetite-regulating hormones by influencing gut microbiota composition. Furthermore, PICRUSt analysis revealed that QBG treatment, particularly Q + _H, could enhance disrupted metabolism and improve gut microbiota functions, helping restore normal physiological function.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·Vol. 104
·期 11
·6584-6593
·
IF 3.3
Junli Lv,Mengmeng Tan,Jiali Zou,Yueying Yun,Yan Ren,Fucheng Guo,Guoze Wang
BACKGROUND The biological functions of ferulic acid (FA) have garnered significant interest but its limited solubility and stability have led to low bioavailability. Hydroxypropyl- β -cyclodextrin (HP- β -CD), with its distinctive hollow structure, offers the potential for encapsulating hydrophobic molecules. The formation of an inclusion complex between FA and HP- β -CD may therefore be a viable approach to address the inherent limitations of FA. To investigate the underlying mechanism of the FA/HP- β -CD inclusion complex formation, a combination of spectral analyses and computer simulation was employed. RESULTS The disappearance of the characteristic peaks of FA in Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) confirmed the formation of an inclusion complex between FA and HP- β -CD. Thermogravimetry-derivative thermogravimetry (TG-DTG) studies demonstrated that the thermal stability of FA was enhanced due to the encapsulation of FA within HP- β -CD. Molecular dynamics simulation also provided evidence that FA successfully penetrated the HP- β -CD cavity, primarily driven by van der Waals interactions. The formation of the complex resulted in more compact HP- β -CD structures. The bioavailability of FA was also strengthened through the formation of inclusion complexes with HP- β -CD. CONCLUSIONS The findings of this study have contributed to a deeper understanding of the interactions between FA and HP- β -CD, potentially advancing a delivery system for FA and enhancing the bioavailability of insoluble active components. © 2024 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Tao Gou,Wenxiu Li,Shenglin Chen,Chao Yi,Yu Guo,Zheng Cao,Lei Zhou,KangJu Lee,Mingrui Chen,Yaowen Liu
In this study, a hydrophobic and antibacterial pad was prepared to preserve Channel Catfish ( Ictalurus punctatus ). The pad composite the microfibrillated cellulose and β-cyclodextrin/nisin microcapsules. The hydrophobic pad ensures a dry surface in contact with the fish, reducing microbial contamination. The pad has a low density and high porosity, making it lightweight and suitable for packaging applications, while also providing a large surface area for antibacterial activity. Results demonstrated that this antibacterial pad exhibits an ultralow density of 9.0 mg/cm 3 and an ultrahigh porosity of 99.10%. It can extend the shelf life of Channel Catfish fillets to 9 days at 4 °C, with a total volatile base nitrogen below 20 mg/100 g. The study proposes a novel solution for preserving aquatic products by combining antibacterial substances with the natural base material aerogel. This approach also extends the utilization of aerogel and nisin in food packaging.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR MEDICINE-JMM
·
2024
·
IF 4.8
Su Qi,Chen Kun,Ren Jiayan,Zhang Yu,Han Xu,Leong Sze Wei,Wang Jingjing,Wu Qing,Tu Kaihui,Sarwar Ammar,Zhang Yanmin
Abstract Non-small cell lung cancer (NSCLC) is a highly malignant tumor with a poor prognosis. Hypoxia conditions affect multiple cellular processes promoting the adaptation and progression of cancer cells via the activation of hypoxia-inducible factors (HIF) and subsequent transcription activation of their target genes. Preliminary studies have suggested that estrogen receptor β (ERβ) might play a promoting role in the progression of NSCLC. However, the precise mechanisms, particularly its connection to HIF-1α-mediated modulation under hypoxia, remain unclear. Our findings demonstrated that the overexpression of ERβ, not ERα, increased cell proliferation and inhibition of apoptosis in NSCLC cells and xenografts. Tissue microarray staining revealed a strong correlation between the protein expression of HIF-1α and ERβ. HIF-1α induced ERβ gene transcription and protein expression in CoCl 2 -induced hypoxia, 1% O 2 incubation, or HIF-1α overexpressing cells. ChIP identified HIF-1α binding to a hypoxia response element in the ESR2 promoter. The suppression of HIF-1α and ERβ both in vitro and in vivo effectively reduced the tumor growth, thus emphasizing the promising prospects of targeting HIF-1α and ERβ as a therapeutic approach for the treatment of NSCLC. Key messages
ERβ, not ERα, increases cell proliferation and inhibition of apoptosis in NSCLC cells and xenografts.
A strong correlation exists between the protein expression of HIF-1α and ERβ.
HIF-1α induced ERβ gene transcription and protein expression in hypoxic cells via binding to HRE in the ESR2 promoter.
The suppression of HIF-1α and ERβ both in vitro and in vivo effectively reduced the NSCLC tumor growth.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Jialei Han,Ziwei Duan,Changjiang Liu,Yadong Liu,Xinyu Zhao,Bo Wang,Shuaishuai Cao,Dalin Wu
19F magnetic resonance imaging (19F MRI) is gaining attention as an emerging diagnostic technology. Effective 19F MRI contrast agents (CAs) for in vivo applications require a long transverse (or spin–spin) relaxation time (T2), short longitudinal (or spin–lattice) relaxation time (T1), high fluorine content, and excellent biocompatibility. Here, we present a novel hyperbranched polymeric 19F MRI CA based on β-cyclodextrin and phosphorylcholine. The influence of the branching degree and fluorine content on T2 was thoroughly investigated. Results demonstrated a maximum fluorine content of 11.85% and a T2 of 612 ms. This hyperbranched polymeric 19F MRI CA exhibited both great biocompatibility against cells and organs of mice and high-performance imaging capabilities both in vitro and in vivo. The research provides positive insights into the synthesis strategies, topological design, and selection of fluorine tags for 19F MRI CAs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Research
·
2025
·
IF 10.7
Lei Xu,Sangyu Hu,Yuzhu Pei,Yutong Zhou,Xiuli Zhang,Linlin Ding,Minhe Cui,Yonglin Zhou,Xuming Deng,Zihao Teng,Jianfeng Wang
The emergence and prevalence of methicillin-resistant Staphylococcus aureus (MRSA) severely compromises conventional β-lactam antibiotics efficacy and poses an extensive global health challenge. Given the close relationship between docosahexaenoic acid (DHA) and metabolic alterations, this study aimed to reveal the novel function of DHA to potentiate β-lactam antibiotics activity through a lipid peroxidation mechanism. Additionally, DHA exhibited significant inhibitory effects on the catalytic function of β-lactamase through interactions with active residues. Herein, the dual-faceted mechanisms of perturbation of lipid metabolism and β-lactamase catalytic inhibition achieved the potentiated antibacterial efficacy of β-lactam antibiotics in combination with DHA against MRSA. Furthermore, to enhance the pharmacodynamic performance and stability of DHA, amoxicillin and DHA co-loaded nanoemulsions (Amo/DHA-NEs) were prepared via high-energy emulsification. Intriguingly, we found that Amo/DHA-NEs effectively rescued MRSA-induced infections in the murine infection models, as evidenced by the superior bacterial clearance and mitigated inflammation. Collectively, this work reveals a potentially exploitable link between DHA-driven metabolic reprogramming and β-lactams resistance, and we propose combination therapies of DHA and β-lactams targeting the emerging threat of MRSA infections.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR NEUROBIOLOGY
·
2024
·
IF 4.6
Wang Zhenhua,Li Yuan,Wang Zhixu,Liao Yuhui,Ye Qingqing,Tang Shilong,Wei Ting,Xiao Pengyu,Huang Juan,Lu Weitian
Oxidative stress (OS) is the main cause of secondary damage following intracerebral hemorrhage (ICH). The polarity expression of aquaporin-4 (AQP4) has been shown to be important in maintaining the homeostasis of water transport and preventing post-injury brain edema in various neurological disorders. This study primarily aimed to investigate the effect of the oxygen free radical scavenger, edaravone, on AQP4 polarity expression in an ICH mouse model and determine whether it involves in AQP4 polarity expression via the OS/MMP9/β-dystroglycan (β-DG) pathway. The ICH mouse model was established by autologous blood injection into the basal nucleus. Edaravone or the specific inhibitor of matrix metalloproteinase 9 (MMP9), MMP9-IN-1, called MMP9-inh was administered 10 min after ICH via intraperitoneal injection. ELISA detection, neurobehavioral tests, dihydroethidium staining (DHE staining), intracisternal tracer infusion, hematoxylin and eosin (HE) staining, immunofluorescence staining, western blotting, Evans blue (EB) permeability assay, and brain water content test were performed. The results showed that OS was exacerbated, AQP4 polarity was lost, drainage function of brain fluids was damaged, brain injury was aggravated, expression of AQP4, MMP9, and GFAP increased, while the expression of β-DG decreased after ICH. Edaravone reduced OS, restored brain drainage function, reduced brain injury, and downregulated the expression of AQP4, MMP9. Both edaravone and MMP9-inh alleviated brain edema, maintained blood–brain barrier (BBB) integrity, mitigated the loss of AQP4 polarity, downregulated GFAP expression, and upregulated β-DG expression. The current study suggests that edaravone can maintain AQP4 polarity expression by inhibiting the OS /MMP9/β-DG pathway after ICH.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Food Science & Technology
·
2024
·
IF 2.6
Haoran Bai,Liying Yang,Zheng Li,Douxin Xiao,Alideertu Dong
The infestation of pathogenic microorganisms leads to a decline in fruit quality and reduces their storage time. The existing methods for contact preservation suffer from the issue of migration of harmful ingredients, thus necessitating the development of novel preservation techniques. The present study focuses on the development of a contactless and biosafe solid preservative (GR@β-CD-MOF) by incorporating geraniol (GR, essential oil) into the β-cyclodextrin MOF (β-CD-MOF). The loading rate of geraniol was 210.8 mg/g. The morphology and structure of GR@β-CD-MOF were characterized using SEM, XRD, XPS, and FTIR. Furthermore, we evaluated the in vitro antimicrobial activity of GR@β-CD-MOF against three common food-borne pathogens Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Botrytis cinerea (B. cinerea). Subsequently, the GR@β-CD-MOF was placed into poly(lactic acid) bags to prepare a solid preservative for emperor bananas. In comparison with the control group, the emperor bananas treated with GR@β-CD-MOF exhibited lower decreases in relevant physicochemical parameters including color, weight loss, and soluble solids content. Moreover, the shelf life of emperor bananas was extended up to 8 days. These findings validate the efficacy of GR@β-CD-MOF as a solid preservative for fruit preservation.
✓ 已验证
产品关联
DOI
高影响因子
INORGANIC CHEMISTRY
·
2025
·
IF 4.7
Yunjie Bai,Cheng Chen,Mingye Han,Bingbing Zhang,Ying Wang
Borate materials possess diverse crystal structures and exhibit outstanding physical properties, making them highly suitable for optical applications. In this work, two new compounds, α-BaGdBO3F2 and β-BaGdBO3F2, were synthesized by the high-temperature solid solution method and a temperature-controlled phase transition from α-BaGdBO3F2 to β-BaGdBO3F2 was observed. In the crystal structures of α-BaGdBO3F2 and β-BaGdBO3F2, the [BO3] basic building unit and the [GdO6F2] polyhedra are interconnected to form a complex three-dimensional network. The title compounds exhibit good thermal stability, a short ultraviolet cutoff of 274 nm, and a moderately large birefringence (0.072 and 0.059@ 546 nm). The DFT calculations also reveal that β-BaGdBO3F2 could be a potential nonlinear optical (NLO) material. The results indicate that α-BaGdBO3F2 and β-BaGdBO3F2 are promising candidates for UV birefringent and NLO crystal materials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2011
·Vol. 29
·期 8
·1753-1758
·
IF 0.773
Xiuling Ma,Zhen Chen,Xiao Chen,Riyao Chen,Xi Zheng
A novel molecularly imprinted composite nanofiber was prepared by a simple electrospinning technique, in which polyvinylbutyral (PVB) was chosen as matrix, β -cyclodextrin ( β -CD) was used as a functional monomer and naringin (NG) as template molecules. After cross-linked by hexamethylene diisocyanate (HMDI), the composite nanofiber exhibited a high specific binding capacity. The morphological structure of the nanofibers was studied by means of infrared spectrum (IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The β -CD molecules were mostly homogeneously distributed within the PVB nanofiber without forming phase separated crystalline aggregates. Compared with traditional imprinted β -CD polymer, the binding experiments demonstrated that the molecularly imprinted composite nanofiber shows the specific binding sites and the selective binding ability for NG. The molecularly imprinted nanofiber could be used at least six times without any loss in binding capacity.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 21
·5023
·
IF 4.2
Xiaoding Lin,Ting Xu,Wenqi Hou,Xiaoyan Dong,Yan Sun
The deposition of amyloid β-protein (Aβ) in the brain is the main pathogenesis of Alzheimer’s disease (AD). The development of potent inhibitors against Aβ aggregation is one of the effective strategies to combat AD. Endogenous transthyretin (TTR) can inhibit Aβ fibrillization via hydrophobic interactions, but its weak inhibitory potency hinders its application in AD therapy. Here, different recombinant TTRs were designed by cationic surface charge engineering. Compared with TTR, all positively charged recombinant TTRs showed enhanced capability in inhibiting Aβ aggregation, especially the recombinant protein obtained by mutating the acidic amino acid in TTR to arginine (TTR-nR) exhibited excellent inhibitory effect. Among them, TTR-7R remarkably increased the inhibitory potency against Aβ, which could effectively inhibit Aβ40fibrillization at a very low concentration (0.5 μM). In addition, TTR-7R increased cultured cell viability from 62% to 89%, scavenged amyloid plaques in AD nematodes, and prolonged nematode lifespan by 5 d at 2 μM. Thermodynamic studies demonstrated that TTR-7R, enriching in positive charges, presented hydrophobic interactions and enhanced electrostatic interactions with Aβ40, leading to a significantly enhanced inhibitory capacity of TTR-7R. The research provided insights into the development of efficient recombinant protein inhibitors for AD treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）