Animals
·
2026
·Vol. 16
·期 11
·1606
·
IF 3.2
Yiping Gu,Hongzuo Duan,Congyun Ji,Hanyu Lin,Yuxin Lai,Jianwei Zhang,Cun Zhang,Suxin Huo,Zheng Ni,Tao Yun,Weicheng Ye,Jionggang Hua,Liu Chen,Yuan Fu,Yinchu Zhu,Zihao Pan
Simple SummaryBovine herpesvirus type 1 (BoHV-1) causes severe respiratory disease and reproductive disorders in cattle, leading to major economic losses worldwide. Current vaccines in China have limitations, as they cannot distinguish vaccinated animals from infected ones. In this study, we developed two new live vaccines by removing three viral genes (rBoHV-∆gG-∆gE/gI and rBoHV-∆TK-∆gE/gI). We tested their safety and effectiveness in rabbits. The results show that both vaccines were safe and induced strong immune responses. After exposure to the virus, vaccinated rabbits showed no fever, significantly reduced virus shedding, and less lung damage. Notably, the rBoHV-∆TK-∆gE/gI vaccine worked best at a high dose, while the rBoHV-∆gG-∆gE/gI vaccine was effective even at a low dose. These findings suggest that our new vaccines could help control BoHV-1 infections and support eradication programs by allowing differentiation between vaccinated and naturally infected animals.Bovine herpesvirus type 1 (BoHV-1) causes severe respiratory and reproductive diseases in cattle, leading to significant economic losses worldwide. Current inactivated vaccines in China fail to induce robust cellular immunity and cannot differentiate infected from vaccinated animals (DIVA). To address these limitations, we constructed two triple-gene-deleted BoHV-1 vaccine candidates, ∆T3 (∆TK-∆gE/gI) and ∆g3 (∆gG-∆gE/gI), using Red/ET two-step recombination technology based on an infectious BAC clone. Their safety and immunogenicity were evaluated in a rabbit model. Both deletion mutants exhibited growth kinetics similar to the wild-type strain but with significantly reduced viral titers and plaque areas. The triple-gene deletion mutants showed superior safety compared with single-gene deletion mutants, with minimal clinical signs and low viral shedding. Immunization with ∆T3 and ∆g3 induced robustgB-specific and neutralizing antibody responses. Following challenge with the virulent BoHV-1 BC01 strain, vaccinated rabbits maintained normal body temperatures, showed significantly reduced viral shedding (approximately 100-fold), and exhibited milder pulmonary lesions. These findings demonstrate that ∆T3 high-dose and ∆g3 low-dose regimens are promising DIVA-compatible vaccine candidates, offering an effective strategy for IBR control and eradication programs.
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MOLECULAR THERAPY
·
2026
·
IF 11.4
Yang Yang,Jiannan Wang,Hong Yu,Zhen Quan,Zhujiao Zhang,Jingran Guo,Haoxuan Wu,Shanshan Liu,Xiaoqian Li,Xinxin Hu,Hongyan Zou,Mengyuan Zhu,Tianyi Wang,Zijian Zhou,Jiaying Liu,Jing Wang,Kai Wang,Lili Yang,Zhaoguo Han,Xilin Sun
The costimulatory receptor CD28 is indispensable for regulating T-cell effector function, and the absence of co-stimulation is associated with poor immunotherapy response. However, longitudinal molecular imaging studies targeting CD28 as a costimulatory biomarker using positron emission tomography (PET) have not been reported. Here, we developed the co-stimulation-associated radiotracer [ 89 Zr]Zr-DFO-anti-CD28 to enable early prediction of therapeutic response by identifying recovery of T-cell responsiveness following programmed cell death protein 1 (PD-1) blockade in lung cancer. [ 89 Zr]Zr-DFO-anti-CD28 demonstrated high specificity for CD28 + tumor-infiltrating lymphocytes (TILs) in vitro and in vivo. By monitoring CD28 expression and its loss within the tumor microenvironment, [ 89 Zr]Zr-DFO-anti-CD28 PET identified responses to anti-PD-1 therapy earlier than changes in tumor volume observed at day 5, and increased radiotracer uptake correlated with more favorable treatment responses. Repeated PET imaging was performed to monitor dynamic changes in CD28 + TILs induced by different immunotherapies at day 14, and [ 89 Zr]Zr-DFO-anti-CD28 PET captured the CD28-dependent restoration of T-cell effector function, further supporting its potential for early prediction. Taken together, costimulatory biomarker-specific CD28 PET molecular imaging may enable noninvasive early prediction of responses to multiple immunotherapies and facilitate the application of precision medicine in lung cancer.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2012
·
IF 2.098
Sheng-Gui Liu,Lin-Ping Zhang,Jian Liu,Wen-Yi Su,Xiao-Bo Shi
Two cadmium complexes, Cd(bpbp)Cl 2 (complex 1 ) and [Cd(bpbp) 2 ](ClO 4 ) 2 (complex 2 ), based on 2,6-bis(1-phenylbenzimidazol-2-yl)pyridine (bpbp), were synthesized and characterized by X-ray single crystal structure analyses. For complex 1 : crystal system, monoclinic, space group, C2/c, a = 27.427(3) Å, b = 13.4495(15) Å, c = 14.8381(17) Å, β = 106.635(2)°, V = 5244.4(10) Å 3 , Z = 8. It is a neutral complex. The Cd(II) ion distorted trigonal bipyramidal geometry is five-coordinated by three nitrogen atoms from ligand (bpbp) and two chlorine ions. For complex 2 : crystal system, triclinic, space group, P-1, a = 13.4791(15) Å, b = 13.8506(16) Å, c = 16.5839(19) Å, α = 94.202(2)°, β = 106.948(2)°, γ = 94.872(2)°, V = 2935.3(6) Å 3 , Z = 2. It is an ionic complex. The Cd(II) ion octahedral geometry is six-coordinated by six nitrogen atoms from two ligand (bpbp). Both complexes emit blue luminescence with emission peaks at 420 and 430 nm in solid state and with emission peaks at 415 and 425 nm in DMF solution. In complex 1 absorption spectra, there is not only the free ligand absorption peak at 310 nm, but also shows strong Cd–Cl charge transfer peak at 350 nm in DMF solution.
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MEDICINE
·
2026
·Vol. 105
·期 5
·e47341
·
IF 1.4
He Mingjie,Wen Jie,Zou Yan,Jiang Qiong,Zhong Aimin
Hyperhomocysteinemia has been identified as a potential metabolic risk factor for type 1 diabetes-related nephropathy (T1DN). However, the causal relationship between homocysteine (Hcy) and T1DN remains unclear. We performed univariable Mendelian randomization (MR) and multivariable MR analyses to investigate the causal effects of Hcy and B vitamins (vitamin B6, B12, and folate) on T1DN in a cohort of 19,406 individuals. Linkage disequilibrium score regression and colocalization analyses were conducted to examine genetic correlations. Additionally, 2-step MR analysis was employed to identify potential mediating factors. Our MR findings revealed that elevated Hcy levels were causally associated with the late stage of T1DN (OR = 1.33, 95% CI: 1.08–1.64; P < .0001; false discovery rate < 0.05). Multivariable MR analysis further confirmed that Hcy remained significantly associated with increased risk of late-stage T1DN (OR = 1.215; false discovery rate < 0.05), even after adjusting for kidney function and vitamin B6 levels. Two-step MR analysis suggested that estimated glomerular filtration rate mediated 3.21% of the effect of Hcy on T1DN. No causal relationship was observed between Hcy and the microalbuminuria stage of T1DN (OR = 0.963, 95% CI: 0.717–1.293). Furthermore, folate, vitamin B6, and vitamin B12 were not significantly associated with T1DN. Experimental results showed that Hcy inhibited HK-2 cell viability and induced cellular injury. Notably, treatment with losartan and sinomenine alleviated Hcy-induced cytotoxicity, indicating their potential protective effects. The findings of this study suggest that elevated Hcy levels represent an important risk factor contributing to the development of T1DN.
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Food Bioscience
·
2026
·
IF 6.2
Shiyu Zhang,Xinzhuan Yao,Yanxia Wang,Muhammad Safiullah Virk,Shiyu Tian,Zhenkedai Yuan,Qihong Zou,Linlin Li,Shenyuan Ye,Hu Tang,Litang Lu
This study comprehensively evaluated Baiye No.1 (BY1H), a temperature-sensitive tea cultivar. Normally, it whitens in early spring and gradually regreens in autumn, accompanied by declining free amino acids and rising catechin and caffeine levels. We reevaluated the common belief that autumn tea is inferior. The feasibility of producing premium yellow tea from autumn-harvested BY1H was assessed, and the mechanisms underlying its quality formation were elucidated. The menhuang yellowing step played a central role. Driven by non-enzymatic thermal oxidation and hydrolysis, it reduced typical autumn defects by lowering phenolic and caffeine levels and increasing sweet-tasting free amino acids. Sensory evaluation, targeted metabolite profiling, and multivariate statistics were integrated to compare BY1H with four reference yellow teas (Ya’an Yellow Tea, Weishan Maojian, Yuan’an Luyuan Yellow Tea, and Pingyang Huangtang). BY1H yellow tea exhibited superior sensory attributes for its mellow-sweet taste and layered floral bouquet. Chemically, this profile was supported by a high water-extract yield, low levels of caffeine and ester-catechins, and abundant serine and phenylalanine. Together, these components contributed to its mellow-sweet taste. Gas Chromatography-Mass Spectrometry (GC-MS) analysis further identified 18 key volatiles, among which benzaldehyde, β-ionone, and methyl 2-(2-methylpropoxy)benzoate were the major contributors to the sweet-floral aroma. Partial Least-Squares Discriminant Analysis (PLS-DA) and Mantel tests confirmed BY1H’s unique chemical signature, clearly separating it from the other traditional yellow teas. These findings demonstrate that autumn BY1H leaves can be valorized through yellow tea processing, providing a scientific basis for cultivar innovation and sustainable utilization of seasonal tea resources.
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Journal of Saudi Chemical Society
·
2016
·Vol. 21
·期 3
·315-323
·
IF 1.978
Pei-Yi Wang,Ling Chen,Jian Zhou,He-Shu Fang,Zhi-Bing Wu,Bao-An Song,Song Yang
In this paper, a variety of 1-aryl-4-hydroxy-1 H -pyrrol-2(5 H )-one derivatives bearing 1,3,4-oxadiazole moiety were designed and synthesized. Preliminary bioassays suggested that these compounds not only exhibited favorable antibacterial activity toward plant pathogenic bacteria including Xanthomonas oryzae pv. oryzae ( Xoo ), Ralstonia solanacearum ( R. solanacearum ), and Xanthomonas axonopodis pv. citri. ( Xac ), but also demonstrated certain curative and protective activities against Tobacco mosaic virus (TMV). Further studies revealed that compounds 8a and 8l exerted the best inhibition effect against Xoo with the half-maximal effective concentration (EC 50 ) values of 8.6 and 7.3 μg/mL, respectively, which were better than that of commercial agricultural antibacterial bismerthiazol (EC 50 = 92.6 μg/mL). Given the above results, this kind of compounds can be further studied and explored as alternative antibacterial agents.
研究领域:
药物化学
·
农业化学
·
抗菌剂
·
病毒学
·
有机合成
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Acta Biomaterialia
·
2026
·
IF 10.4
Yihui Sun,Yingshuai Wang,Qianbo He,Min Zhao,Jiaru Guo,Chunjie Xu,Qiupeng Yan,Yanchun Chen,Bingqiang Zhang,Hongmei Du,Jing Huo,Yong Zhang,Haoyun Zhang,Yingjun Guan,Qianjun He
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive motor neuron degeneration in the brain and spinal cord, with mutant superoxide dismutase 1 (SOD1) induced oxidative stress and neuroinflammation as key pathogenic drivers. Here, we uncover that mutant SOD1 is both a Fenton-like agent able for catalytical generation of ·OH and a hydrogenation catalyst for H 2 scavenging reactive oxygen species. To enhance the bioavailability of H 2 , we develop an orally administered Mg 2 Si nanosheets based feed for sustained release of high-amount H 2 . On an ALS model of hSOD1 G93A transgenic mice, Mg 2 Si feed remarkably delays ALS progression, improves the motor performance of ALS mice, and extends their lifespan. Histopathologically, oral Mg 2 Si treatment ameliorates motor neuron degeneration, misfolded SOD1 aggregation and reactive gliosis in spinal cord, while protecting neuromuscular junctions and ameliorating muscle atrophy during disease progression. Transcriptomic analysis demonstrates the H 2 -mediated down-regulation of both oxidative stress and neuroinflammatory pathways in response to the suppression of NLRP3 inflammasome activation. The proposed strategy of catalyzed hydrogen therapy offers an inspiration for metalloproteases-related neurodegenerative diseases treatment. Statement of Significance Amyotrophic lateral sclerosis (ALS) is an incurable and devastating neurodegenerative disease lacking effective clinical interventions. Although hydrogen gas (H 2 ) exhibits promising neuroprotective potential, conventional H 2 therapy is severely limited by unstable and transient H 2 release, failing to sustain long-term treatment requirements for chronic ALS pathogenesis. To overcome this bottleneck, we engineer oral administrable Mg 2 Si nanosheets that enable sustained H 2 release via gastrointestinal retention, achieving stable long-term hydrogen supplementation in vivo . Mechanistically, Mg 2 Si-derived H 2 efficiently eliminates excess free radicals triggered by toxic mutant SOD1, and further disrupts the pathological crosstalk between oxidative stress and neuroinflammation in ALS. In transgenic ALS mice, dietary Mg 2 Si intervention markedly ameliorates motor dysfunction and effectively delays disease progression. Collectively, this study firstly applies Mg 2 Si nanomaterial-based sustained hydrogen therapy for ALS treatment, establishes a novel gastrointestinal hydrogen delivery strategy, and provides an innovative and clinically translatable paradigm for the design of hydrogen delivery systems against neurodegenerative disorders. Download: Download high-res image (244KB) Download: Download full-size image
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Fermentation-Basel
·
2023
·Vol. 9
·期 2
·133
·
IF 3.7
Peng Cheng,Muhammad Usman,Muhammad Arslan,Huanqing Sun,Li Zhou,Mohamed Gamal El-Din
A Gram-positive exoelectrogenRhodococcus pyridinivoranssp. strain HR-1 was cultivated from leachate-fed microbial fuel cell (MFC) to study the biodegradation effect of pyridine. In the comparison with mixed cultured MFC, HR-1 presented a remarkable electrical capacity with a maximum output of 4.33 W/m3under 30 °C in neutral anolyte with 1 g/L acetate as a substrate. Further, HR-1 demonstrated the environmental resistance as a Gram-positive strain. Microbial metabolism was evident at pH between 5–9 and temperature in the range of 20–40 °C, whereas optimal condition for pyridine degradation was observed at 30 °C. This is the first study reporting the degradation of pyridine in the bio-electrochemical system that achieved a 42% ± 5% degradation rate in a full operation cycle at 2 g/L of the concentration. Considering the nonnegligible internal resistance of HR-1-inoculated MFC, trehalose lipid was also introduced as a bio-surfactant to reduce the charge transfer obstacle between the microbes and the electrodes. The surface morphology illustrated that the strain had a plump shape with a high specific area. Accordingly, bio-surfactant addition promoted the anode biomass (1.2 ± 0.1 mg/cm2to 1.7 ± 0.2 mg/cm2) and achieved a higher degradation rate (68% ± 4%). The feasibility of electrochemical disposal on pyridine and eminent adaptability of strain sp. HR-1 as a Gram-positive exoelectrogen makes MFC a practical approach for real application.Keywords:microbial fuel cells;gram-positive exoelectrogen;pyridine;trehalose lipid;Rhodococcus pyridinivorans
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2023
·
IF 6
Yongdi He,Jinou Song,Jiuzhong Yang,Yuan Zhuang,Liang Qiu,Zhijun Li,Shubao Song,Jibiao Xie
An experimental study of 5% 1 -nitropropane (1-NP)-initiated cyclohexane pyrolysis was carried out in a flow reactor at 30 Torr (4.0 kPa) over a temperature range of 673–1273 K. The pyrolysis species were detected with synchrotron vacuum ultraviolet photoionization mass spectrometry (SVUV-PIMS) technique. Pyrolysis species from m/z 15–106, including C 1 -C 8 hydrocarbons, CO, NO, NO 2 , CH 2 O, CH 3 OH and C 3 H 5 NO, were identified with near-threshold photoionization efficiency (PIE) spectra and quantified at different temperatures. The experimental results indicated that the addition of 1-NP obviously reduced the initial pyrolysis temperature of cyclohexane from 1048 to 1098–723 K, and caused the decreases of the initial formation temperatures for the species from cyclohexane pyrolysis. Almost all the high-temperature pyrolysis species in the mechanisms proposed in the literature were identified in the experiments. Based on the experimental results in this work and rate coefficients proposed in the literature, the primary pyrolysis mechanisms of cyclohexane in the presence of 1-NP were discussed. The 1-NP pyrolysis occurs at a lower temperature than the cyclohexane does, and produces HONO, NO 2 , C 3 H 7 , etc. It was demonstrated that cyclohexane was mainly consumed via the H-atom abstractions by H and OH attacks in this work and the cyclohexyl radical formed via H-atom abstractions mainly isomerized into 1-C 6 H 11 -6 radical, which further combines with H to form 1-C 6 H 12 .
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Frontiers in Pharmacology
·
2022
·
IF 5.988
Xiao Man,Chen Bocen,Niu Kun,Long Ziyu,Yang Fan,Xie Yiqiang
Background: Alpiniae Oxyphylla Fructus (AOF) is Traditional Chinese medicine and a dietary supplements for centuries, which posseses cardiotonic, neuroprotective, antioxidant, warming the kidney and nourish the spleen, these biological fuction is related to potential anti-aging properties. However, little is known about their effects on aging. This work aimed to investigate the effects of extracts of AOF on longevity and stress resistance in Caenorhabditis elegans (C. elegans) and the mechanisms that underlie its effects.Methods: Wild-type (WT) strand of C.elegans (N2)worms were cultured in growth medium with or without AOF. First, we examined the effects of AOF on lifespan, reproduction and healthspan assay, stress resistance and oxidative analysis, lipofuscin levels. Second, The levels of ROS and MDA, the antioxidant enzyme activities were examined to explore the underlying mechanism of AOF. Finally, the expression of the longevity-related genes were investigated to further understand the AOF’s underlying mechanism.Results: The lifespan of C. elegans was prolonged by 23.44% after treatment with high-dose AOF (100 ug/ml). AOF alleviated aging-related declines in C. elegans health and enhanced resistance to heat shock. Furthermore, AOF decreased reactive oxygen species and malondialdehyde, increased the activities of superoxide dismutase and catalase, and reduced accumulation of fat. AOF upregulated the expression of sod-3, gst-4, daf-16, and skn-1 but downregulated the expression of daf-2 and age-1 and accelerated the translocation of DAF-16 into the nucleus. The extended lifespan induced by AOF was reversed in daf-16(mu86) and skn-1(zu135) mutants, indicating that this gene is involved in AOF-regulated longevity.Conclusion: Our findings demonstrated that AOF extends lifespan and healthspan and enhances stress via boosting the activity of the antioxidant enzyme and controlling the expression of genes associated with insulin/IGF signaling and SKN-1 pathways. As a result, this work suggested AOF as a possible candidate to reduce the signs of aging by activating and inhibiting target genes.
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CELL BIOLOGY INTERNATIONAL
·
2022
·Vol. 47
·期 3
·598-611
·
IF 4.473
Yangyang Wu,Shuo Zhou,An Zhao,Yuling Mi,Caiqiao Zhang
Oxidative stress is a major cause of ovarian aging and follicular atresia. There is growing evidence that showed potential roles of rutin in antidiabetic, anti-inflammatory, antitumor, antibacterial and antioxidant, although it is yet unclear what the underlying mechanism is. Here, we looked into the potential effects of rutin on oxidative stress in the prehierarchical small white follicles (SWFs) from 580-day-old (D580) laying chickens. According to the findings, aging D580 layer ferroptosis was much higher than it was for laying hens during the peak period (280-day-old, D280). In both naturally aged and d -gal-induced chicken SWFs, rutin treatment concurrently boosted cell proliferation and prevented apoptosis. In addition, rutin inhibited the increased ferroptosis in aging hens. Meanwhile, rutin markedly suppressed the elevated ferroptosis and descending antioxidant capacity of D280-culture-SWFs from chicken elicited by d -gal. Rutin's activation of the Nrf2/HO-1 pathway hastened the SWFs' verbal battle with oxidative damage and reduced ferroptosis. Furthermore, activation of the ferroptosis signal increased the oxidative damage in SWFs. In conclusion, rutin alleviated oxidative stress that was induced by ferroptosis in aging chicken SWFs through Nrf2/HO-1 pathway. These findings point to a novel mechanism by which rutin protects SWFs from oxidative stress by suppressing ferroptosis, which is presumably a fresh approach to slowing ovarian aging in laying hens.
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Polymers
·
2023
·Vol. 15
·期 4
·810
·
IF 5
Zeping Wang,Binjie Hu,Haibin Yu,George Zheng Chen
In this study, three copolymers of poly(methyl methacrylate) and poly(butyl acrylate) (PMMA-co-PBA) latex containing 1-octyl-3 methylimidazolium hexafluorophosphate (C8mimPF6), cellulose nanocrystals (CNCs), and C8mimPF6-CNCs were successfully synthesized through mini emulsion polymerization. These novel composites were each coated on mild steel panels and tested for their anti-corrosion performance by immersion of the coated samples in 3.5 wt% sodium chloride (NaCl) solution over a certain period. The synergistic anti-corrosion effects of the C8mimPF6-CNCs sample led to the highest coating resistance, charge transfer resistance, and corrosion inhibition efficiency and the lowest diffusion coefficient and corrosion rate. The proposed synergistic mechanism revealed that CNCs enhanced the barrier effect of the coating while C8mimPF6inhibited corrosion when released.Keywords:ionic liquid;cellulose nanocrystals;corrosion protection;polymer;electrochemical analysis;composite
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危险品化学品经营许可证（带存储）