TALANTA
·
2014
·
IF 3.511
Lina Zou,Yinfeng Li,Shaokui Cao,Baoxian Ye
DNA–polyaniline (PAn) complex Langmuir–Blodgett film modified glassy carbon electrode (GCE) was used as a new voltammetric sensor (DNA/PAn-LB/GCE) for xanthine (XA) detection. The characteristic of DNA/PAn-LB film was studied by electrochemical impedance spectroscopy and scanning electron microscope. Electrochemical behaviors of XA at the sensor were studied in pH 7.0 phosphate buffer solutions by cyclic voltammetry and differential pulse anodic voltammetry. The results showed that this new modified electrode exhibited an excellent immunity from uric acid and hypoxanthine interference and a new sensitive and selective electroanalytical method for XA was proposed with wider linear range. Under the optimum conditions, the calibration curve for XA was obtained over the range of 7.0×10 −8 –1.0×10 −5 mol L −1 , with the detection limit of 3.0×10 −8 mol L −1 . The practicability of this method was demonstrated by determining the concentration of XA in human serum samples.
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BIOSENSORS & BIOELECTRONICS
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2017
·
IF 7.78
Fenglei Gao,Taotao Fan,Shanshan Ou,Jing Wu,Xing Zhang,Jianjun Luo,Na Li,Yao Yao,Yingfeng Mou,Xianjiu Liao,Deqin Geng
In this paper, we reported a sensitive and selective electrochemical method for quantify DNA methylation , analyzing DNA MTase activity and screening of MTase inhibitor based on silver nanoparticles (Ag NPs) decorated carbon nanocubes (CNCs) as signal tag. The Ag NPs/CNCs was prepared by in situ growth of nanosilver on carboxylated CNCs and used as a tracing tag to label antibody. The sensor was prepared by immobilizing the double DNA helix structure on the surface of gold electrode. When DNA MTase was introduced, the probe was methylated. Successively, anti-5-methylcytosine antibody labeled Ag NPs/CNCs was specifically conjugated on the CpG methylation site. The electrochemical stripping signal of the Ag NPs was used to monitor the activity of MTase. The electrochemical signal has a linear relationship with M.SssI activities ranging from 0.05 to 120 U/mL with a detection limit of 0.03 U/mL. In addition, we also demonstrated the method could be used for rapid evaluation and screening of the inhibitors of MTase. The newly designed strategy avoid the requirement of deoxygenation for electrochemical assay, and thus provide a promising potential in clinical application.
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ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Guobin Mao,Qin Cai,Fubing Wang,Changliang Luo,Xinghu Ji,Zhike He
As the blood glucose concentration is an important clinical parameter of diabetes, the rapid and effective detection of blood glucose is very significant for monitoring and managing diabetes. Here, a facile method to prepare Rox-DNA functionalized CdZnTeS quantum dots (QDs) was developed. The Rox-DNA functionalized CdZnTeS QDs were prepared by a one-pot hydrothermal method through phosphorothioate DNA bound to QDs, which were employed as a ratiometric fluorescent probe for the rapid and sensitive detection of H2O2 and glucose. Compared with the traditional multistep construction of ratiometric fluorescent probes, this presented approach is simpler and more effective without chemical modification and complicated separation. The CdZnTeS QDs with green fluorescence is specifically sensitive to H2O2, while the red fluorescence of Rox is invariable. H2O2 is the product from the oxidation of glucose catalyzed by glucose oxidase (GOx). Therefore, a facile method to detect H2O2 and glucose with a detection limit of 0.075 μM for H2O2 and 0.042 μM for glucose was developed. In addition, this proposed probe has been employed for the detection of glucose in human serum with a satisfactory result. Moreover, this probe has been used for visual detection, and the health and diabetics can be distinguished by the naked eye. Meanwhile, this nanoprobe is also generalizable and can be extended to the detection of many other H2O2-mediated analytes.
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MOLECULES
·
2016
·Vol. 21
·期 8
·1061
·
IF 2.465
Chongshan Dai,Daowen Li,Lijing Gong,Xilong Xiao,Shusheng Tang
Furazolidone (FZD), a synthetic nitrofuran derivative, has been widely used as an antibacterial and antiprotozoal agent. Recently, the potential toxicity of FZD has raised concerns, but its mechanism is still unclear. This study aimed to investigate the protective effect of curcumin on FZD-induced cytotoxicity and the underlying mechanism in human hepatocyte L02 cells. The results showed that curcumin pre-treatment significantly ameliorated FZD-induced oxidative stress, characterized by decreased reactive oxygen species (ROS) and malondialdehyde formation, and increased superoxide dismutase, catalase activities and glutathione contents. In addition, curcumin pre-treatment significantly ameliorated the loss of mitochondrial membrane potential, the activations of caspase-9 and -3, and apoptosis caused by FZD. Alkaline comet assay showed that curcumin markedly reduced FZD-induced DNA damage in a dose-dependent manner. Curcumin pre-treatment consistently and markedly down-regulated the mRNA expression levels of p53, Bax, caspase-9 and -3 and up-regulated the mRNA expression level of Bcl-2. Taken together, these results reveal that curcumin protects against FZD-induced DNA damage and apoptosis by inhibiting oxidative stress and mitochondrial pathway. Our study indicated that curcumin may be a promising combiner with FZD to reduce FZD-related toxicity in clinical applications.Keywords:curcumin;furazolidone;oxidative stress;DNA damage;mitochondrial pathway
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Journal of Materials Chemistry B
·
2016
·Vol. 5
·期 1
·123-133
·
IF 4.872
Jingwen Ma,Yun Zeng,Yongchun Liu,Daocheng Wu
Using 4,7-diphenyl-1,10-phenanthroline (DIP) as a main ligand and ethyl cyanoacrylate (ECA) as both an auxiliary ligand and a polymer skeleton, polymer iridium(III) complexes (DIP)2Ir(ECA) and their nanomicelles with aggregation-induced phosphorescence emission (AIPE) activity were synthesized. The morphology, chemical structure and composition of (DIP)2Ir(ECA) nanomicelles were characterized using transmission electron microscopy, size distribution/zeta potential analysis, nuclear magnetic resonance, and Fourier transform infrared spectroscopy. Their AIPE-active effects and double-stranded DNA (dsDNA) monitoring abilities were determined using phosphorescence spectroscopy using a spectrophotofluorometer. The results showed that (DIP)2Ir(ECA) nanomicelles had a good thermostability within 0–100 °C and their size distribution was 29.14 ± 1.46 nm. These nanomicelles showed AIPE-active effects and their phosphorescence intensity increased nearly 30-fold in water compared to in acetone. These nanomicelles could be used in AIPE-active intracellular imaging and dsDNA monitoring. Owing to the specific phosphorescence quenching that occurred when dsDNA encountered (DIP)2Ir(ECA) nanomicelles, thermostable (DIP)2Ir(ECA) nanomicelles could quickly detect dsDNA with high sensitivity and could be conveniently applied not only in monitoring DNA degradation in a wider pH range (specifically in an acidic environment), but also during PCR procedures. More importantly, both (DIP)2Ir(ECA) nanomicelles and immobilized DNase I could be recycled and utilized at least four times using our novel phosphorescence “quenching-recovery” dsDNA detection procedure. The polymeric (DIP)2Ir(ECA) nanomicelles were fast, sensitive, and convenient in monitoring dsDNA and could be recycled four times owing to their thermostability, indicating their great potential in biomedical and environmental applications.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yanmei Li,Junmei Nie,Xinyu Li,Ni Yan,Renjiang Kong,Jiaqi Huang,Yeyang Wu,Zhuofeng Li,Yingtao Zhong,Xin Chen,Hong Cheng
Stimulator of interferon genes (STING) plays an essential role in initiating the innate anti-tumor immunity, which could be activated by the cytosolic DNA but suppressed by the immunosuppressive cells. In this work, a self-delivery ternary immunostimulant (designated as ImmSt) is fabricated for colorectal cancer therapy with the immunological cascades of photodynamic DNA damage and T regulatory cell inhibition. Among which, the photosensitizer of Chlorin e6 (Ce6), the STING agonist of 5,6-dimethylxanthine-4-acetic acid (DMXAA) and the phosphatidylinositol 3-kinases (PI3Ks) inhibitor of ZSTK474 could self-assemble into uniform nanomedicine without drug excipient, which enhances the drug stability, bioavailability and pharmacological activities. Importantly, the photodynamic therapy (PDT) effect of ImmSt produces enormous reactive oxygen species to ablate tumor cells, and also triggers the release of cytosolic DNA to amplify the STING signal in combination with the STING agonist. Moreover, ImmSt is able to induce a systemic anti-tumor immunity by the cascade activation of T cells and the decrease of T regulatory cells. In vitro and in vivo results confirm that ImmSt can efficiently eradicate colorectal cancer without severe side effects, which may provide a sophisticated mechanism for immunotherapy in consideration of the immune activation and immunosuppressive factors.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2017
·
IF 2.673
Tian-Fu He,Zhi-Hong Zhang,Xin-An Zeng,Lang-Hong Wang,Charles S. Brennan
This work was aimed to investigate the antibacterial action of cinnamaldehyde (CIN) against Escherichia coli ATCC 8735 ( E. coli ) based on membrane fatty acid composition analysis, alterations of permeability and cell morphology as well as interaction with genomic DNA . Analysis of membrane fatty acids using gas chromatography–mass spectrometry (GC–MS) revealed that the proportion of unsaturated fatty acids (UFA) and saturated fatty acids (SFA) were the major fatty acids in plasmic membrane, and their levels were significantly changed after exposure of E. coli to CIN at low concentrations. For example, the proportion of UFA decreased from 39.97% to 20.98%, while the relative content of SFA increased from 50.14% to 67.80% as E. coli was grown in increasing concentrations of CIN (from 0 to 0.88 mM). Scanning electron microscopy (SEM) showed that the morphology of E. coli cells to be wrinkled, distorted and even lysed after exposure to CIN, which therefore decreased the cell viability . The binding of CIN to genomic DNA was probed using fluorescence, UV–Visible absorption spectra, circular dichroism, molecular modeling and atomic force microscopy (AFM). Results indicated that CIN likely bound to the minor groove of genomic DNA, and changed the secondary structure and morphology of this biomacromolecule. Therefore, CIN can be deem as a kind of natural antimicrobial agents, which influence both cell membrane and genomic DNA.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Chen Yiting,Gong Chunhui,Chen Kaiwei,Wang Ziwei,He Manli,Wang Peng,Chen Kai,Jiao Yan,Yang Yi
Strontium-90 ( 90 Sr) is a major radioactive component that has attracted great attention, but its detection remains challenging since there are no specific energy rays indicative of its presence. Herein, a biosensor that is capable of rapidly detecting Sr 2+ ions is demonstrated. Simple colorimetric method for sensitive detection of Sr 2+ with the help of single-stranded DNA was developed by preparing MnO 2 nanorods as oxidase mimic catalysis 3,3′,5,5′-tetramethylbenzidine (TMB). Under weakly acidic conditions, MnO 2 exhibited a strong oxidase-mimicking activity to oxidize colorless TMB into blue oxidation products (oxTMB) with discernible absorbance signals. Nevertheless, the introduction of a guanine-rich DNA aptamer inhibited MnO 2 -mediated TMB oxidation and reduced oxTMB formation, resulting in blue fading and diminished absorbance. Upon the addition of strontium ions to the system, the aptamers formed a stable G-quadruplex structure with strontium ions, thereby restoring the oxidase-mimicking activity of MnO 2 . Under the best experimental conditions, the absorbance exhibits a linear relationship with the Sr 2+ concentration within the range 0.01–200 μM, with a limit of detection of 0.0028 µM. When the concentration of Sr 2+ from 10 −8 to 10 −6 mol L −1 , a distinct color change gradient could be observed in paper-based sensor. We successfully applied this approach to determine Sr 2+ in natural water samples, obtaining recoveries ranging from 97.6 to 103% with a relative standard deviation of less than 5%. By providing technical solutions for detection, our work contributed to the effective monitoring of transportation of radioactive Sr in the environment. Graphical abstract
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Danni Jiang,Yang Yang,Xiaojuan Han,Qianni Li,Yingxiu Ma,Jun Jiao,Lan Chao
Fluorene-9-bisphenol (BHPF), a widely recognized alternative to bisphenol A (BPA), has been increasingly used in a wide range of industries. However, despite numerous studies, the direct mechanism underlying its male reproductive toxicity remains poorly understood. Here, a BHPF-exposed mouse model was established to evaluate the effect of BHPF on male fertility. The results showed that BHPF exposure induced reproductive dysfunction, including testis damage, spermatogenesis impairment, steroid hormone disruption and sperm quality degradation. Mechanistically, over 90,000 differentially methylated regions were identified and increased global testicular methylation levels indicated that DNA methylation may be associated with BHPF-induced testicular damage. Transcriptome analysis revealed 221 up-regulated genes and 227 down-regulated genes in BHPF-exposed mice, mainly enriched in the steroid biosynthetic pathway. Further combined methylome and transcriptome analysis revealed the critical methylated genes potentially involved in lipid metabolism. The differentially expressed genes (Cyp4a10 and Rdh1) were further confirmed to correlate with impaired male mouse fertility. Taken together, the results of this study provide a better understanding of the molecular mechanisms of toxicity effects induced by BHPF exposure from the perspective of methylome and transcriptome.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yingyi Wang,Shanshan Wang,Chunsong Lu,Xiaoming Yang
Malignant tumors posed severe threat to human health, and various efforts have been contributed towards assaying tumor markers, howbeit, achieving to identify the multiple targets by one single analytical mode is in demand. Hereby, three kinds of aptamer-functionalized silver and silver/gold nanoclusters (DNA-AgNCs and DNA-Ag/AuNCs) were originally synthesized, and possessed the specific recognition ability for their corresponding targets. Specifically, the fluorescence of AgNCs or Ag/AuNCs was quenched by introducing graphene oxide (GO), and more competitive interactions between tumor markers and their aptamers occurred with the targets added, thus facilitating their fluorescence recovered. Based on AgNCs or Ag/AuNCs cooperating with GO, this sensing mode was subsequently employed for detecting mucin 1 in a linear range of 1.33–200 ng/mL with a detection limit of 0.18 ng/mL. Meanwhile, this method was also applied to examine carcinoembryonic antigen and cancer antigen 125, and showed the linear range of 6.7 ng/mL–13.3 μg/mL with a detection limit of 3.18 ng/mL for CEA, while CA125 sensing acquired a linear range of 2 ng/mL–6.7 μg/mL accompanied by a detection limit of 1.26 ng/mL. Besides, this strategy provided the potential ways to identify other tumor markers by designing their corresponding nanoclusters with the aptamers.
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TALANTA
·
2017
·
IF 4.162
Lu Ding,Chunlan Xiang,Gang Zhou
Poly(acrylic acid) (PAA) brushes coated onto silica nanoparticles have been widely utilized in bioassays due to their abilities of providing favorable microenvironments and ensuring good biological activities for biomolecules. However, traditional PAA brushes are synthesized by reversible addition–fragmentation chain transfer polymerization. Hence, it is generally difficult to control and characterize the molecular weight of the PAA brushes, which may depress the reproducibility and bring more uncertain results. Herein, atom transfer radical polymerization method is employed to synthesize β -cyclodextrin-cored PAA with uniform and controllable molecular weight. After loading on the surfaces of adamantane-functionalized silica nanoparticles via host-guest interactions, glucose oxidase and probe single strand DNA (ssDNA) are further immobilized on the as-prepared nanoparticles. Meanwhile, capture ssDNA is functionalized on amino modified magnetic beads. In the presence of ssDNA sequence of Hepatitis B Virus (HBV) containing completely matched sequence of both probe and capture ssDNA, a bioconjugate is formed and can be separated by an external magnet. The isolated glucose oxidase can further catalyze glucose into gluconic acid and H 2 O 2 , and then reduce HAuCl 4 on Au seeds. By monitoring the absorption intensity change of the Au NPs at 530 nm, the proposed biosensor with novel signal amplification probes can be used to detect DNA sequence of HBV with high sensitivity and selectivity in both buffer and serum samples. This developed strategy has presented a new way to construct silica nanoparticles coated by PAA brushes for the fields of clinical diagnosis and other life sciences.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Sun Rengang,Zhou Yadong,Fang Yunzhu,Qin Yirui,Zheng Yekai,Jiang Li
The harm and impact of the COVID-19 pandemic have highlighted the importance of fast, sensitive, and cost-effective virus detection methods. In this study, we developed a DNA aptamer sensor using nanoparticle-enhanced surface plasmon resonance imaging (SPRi) technology to achieve efficient labeling-free detection of SARS-CoV-2 S protein. We used the same DNA aptamer to modify the surface of the SPRi sensor chip and gold nanoparticles (AuNPs), respectively, for capturing target analytes and amplifying signals, achieving ideal results while greatly reducing costs and simplifying the preparation process. The SPRi sensing method exhibits a good linear relationship ( R 2 = 0.9926) in the concentration range of 1–20 nM before adding AuNPs to amplify the signal, with a limit of detection (LOD) of 0.32 nM. After amplifying the signal, there is a good linear relationship ( R 2 = 0.9829) between the concentration range of 25–1000 pM, with a LOD of 5.99 pM. The simulation results also verified the effectiveness of AuNPs in improving SPRi signal response. The SPRi sensor has the advantage of short detection time and can complete the detection within 10 min. In addition, the specificity and repeatability of this method can achieve excellent results. This is the first study to simultaneously capture a viral marker protein and amplify the signal using polyadenylic acid (polyA)–modified DNA aptamers on the SPR platform. This scheme can be used as a fast and inexpensive detection method for diagnosis at the point of care (POC) to combat current and future epidemics caused by the virus.
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