TALANTA
·
2026
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IF 6.7
Ziyi Chen,Yaqian Feng,Shu Chen,Wenfu Liu,Peisheng Zhang,Rongjin Zeng,Yuanqiang Hao
This study presents a polarity-switchable photoelectrochemical (PEC) method for determining hydrogen sulfide (H 2 S) in fruit matrices by electrochemically programming BiOI into a BiOI/Bi 2 S 3 heterojunction on FTO. BiOI nanosheets were first deposited potentiostatically and then partially sulfidized in Na 2 S under a mild cathodic bias, forming a tightly coupled BiOI/Bi 2 S 3 heterostructure with an in-situ generated surface Bi 2 S 3 layer. The heterojunction broadens visible-light absorption and strengthens interfacial charge separation, leading to a cathodic-to-anodic photocurrent inversion that greatly improves signal discrimination at 0 V bias. Under optimized conditions, the device shows a linear range of 0.05–30 μM with a 5.42 nM LOD, high selectivity, and stable on/off cycling. Applied to real samples, H 2 S levels of 2.13, 1.54, and 1.32 μM were obtained for apple, pear, and kiwifruit, with 93.8%‒107.4% recoveries by standard additions. This simple, low-bias PEC platform couples in-situ structural conversion with polarity switching for rapid quality monitoring and early spoilage assessment.
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BIOLOGICAL TRACE ELEMENT RESEARCH
·
2026
·
IF 4.3
Zhang Zhenyang,Chen Weijun,Shen Jiaqi,Dong Haowei,Zhang Chunnuan,Gao Xiaochan,Ren Hongtao,Huang Yong,Gao Shiyang
Waterborne cadmium (Cd) remains a significant threat to the intestinal health and growth of largemouth bass ( Micropterus salmoides ), necessitating the search for effective dietary mitigants. In this 56-day trial, the protective efficacy of paeonol (PAE) was evaluated across five experimental groups: a negative control, a Cd-stressed group (Cd, 2.75 mg/L waterborne exposure), and three treatment groups concurrently exposed to 2.75 mg/L Cd and supplemented with dietary PAE at 100, 200, and 400 mg/kg, respectively. The results showed that Cd exposure induced intestinal oxidative stress, impaired structural integrity, downregulated antimicrobial peptide expression, compromised immune function, and disrupted intestinal microbial homeostasis ( P < 0.05). PAE supplementation significantly decreased malondialdehyde (MDA) content, and the PAE200-Cd exhibited increased activities of total superoxide dismutase (T-SOD) and catalase (CAT), as well as elevated expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and cytochrome P450 1A1 (CYP1A1) ( P < 0.05). Intestinal villus structure was restored, the number of goblet cells increased, and the expression of tight junction proteins (Occludin, ZO-1, Claudin-1) genes was significantly upregulated ( P < 0.05). Meanwhile, the expression of β-defensin, Lysozyme, and Mucin 2 was significantly upregulated ( P < 0.05). The PAE100-Cd showed increased expression of immunoglobulins (e.g., IgM and IgT), while the PAE200-Cd inhibited the expression of pro-inflammatory factors ( P < 0.05). Cd-induced negative changes in intestinal microbial diversity and stability were restored by PAE, and the PAE200-Cd exhibited decreased abundances of Proteobacteria and Pseudomonas , and increased abundance of Firmicutes ( P < 0.05). Under the present experimental conditions, the appropriate dietary PAE level based on oxidative stress and intestinal physical barrier was preliminarily estimated to be 115.51–229.55 mg/kg. Collectively, PAE can alleviate Cd-induced intestinal injury by improving antioxidant capacity and enhancing intestinal barrier function, and represents a promising functional candidate for heavy metal mitigation. Graphical The alternative text for this image may have been generated using AI.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Qiongya Lu,Jingcheng Hu,Xing Ni,Xue Zhang,Jiaojiao Xia,Yani Zhao,Rubing Huang,Bin Zou
In this study, we successfully synthesized two immobilized enzyme carrier: hollow mesoporous carbon spheres (HMCS) and mesoporous carbon spheres (MCS). The carriers were thoroughly characterized by using a range of techniques, including scanning electron microscopy, water contact angle measurement, and BET surface area analysis. HMCS exhibited a unique hollow structure, moderate hydrophobicity, and a larger specific surface area. The high-performance immobilized lipase, CRL@HMCS was prepared by using HMCS as the carrier. The basic enzymatic properties and reaction kinetic parameters of CRL@HMCS were studied. CRL@HMCS exhibited significantly higher catalytic efficiency, with a Vmax approximately 2.7-fold greater than that of the free CRL. Excellent operational stability was showed by retaining 64.14% of its relative activity after being reused 7 times and 84.05% after 22 days of storage. Under optimal conditions (45 °C, 200 rpm, 5 h), CRL@HMCS achieved a 70.4% yield in geranyl acetate synthesis, significantly higher than the free enzyme (48.65%).
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Lu Han,Qingqing Pan,Mengting Chen,Xi Fan,Bin He,Yuji Pu
The development of oral drug delivery systems with colon-targeted release and high biocompatibility is of critical importance for the treatment of ulcerative colitis (UC), considering the chronic nature of the disease and the genetic susceptibility of patients. Herein, we report a composite delivery system (UA@PB/Gel) based on Generally Recognized As Safe (GRAS) materials, poly(vinyl alcohol) (PVA) and inulin, designed for colon-specific delivery of ursolic acid (UA). In this system, butyrate-conjugated PVA nanoparticles efficiently encapsulate UA and respond to colonic esterase, enabling the localized release of both UA and butyrate. Embedding these nanoparticles within an inulin hydrogel further enhances colonic retention and provides a sustained release. The composite system demonstrates efficient colon-targeting delivery, prolonged retention, and potent anti-inflammatory effects in vitro and in vivo. In a dextran sulfate sodium-induced colitis mouse model, UA@PB/Gel effectively alleviates colonic inflammation, restores epithelial barrier integrity, reduces proinflammatory cytokine expression, and modulates gut short-chain fatty acid levels, with minimal systemic toxicity. These results highlight the potential of the GRAS materials-based nanoparticle–hydrogel composite as a safe and effective therapeutic platform for UC.
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ACS Omega
·
2026
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IF 5.2
Ling Chen,Li Huang,Xingbo Yan,Panpan Zhang,Enben Su,Yu Zhang
Although a variety of novel strategies have been used to select antibody-producing cells efficiently either from natural B cell collections or from plasma cell collections postimmunization, the production of monoclonal antibodies against weak antigens with high affinity and special epitopes for diagnostic or therapeutic applications remains fraught with challenges and uncertainty. In this report, we proposed an effective method for generating monoclonal antibodies from rabbits by using histone deacetylase inhibitor (HDACi) to improve antibody gene diversity, particularly the diversity of the complementarity-determining regions (CDRs) which determines the specificity of the antibody. HDACi was encapsulated in nanoliposomes to form an immunomodulator, which was administered to neonatal rabbits during the ontogeny of the immune systems. It was observed that the number of antigen-specific plasma cells in these treated rabbits was increased, and more importantly, the antibody gene diversity, especially the CDRs, was proven to have increased after 3 times of antigen immunization compared with the untreated group, probably due to the promoted efficiency of gene conversion. The results indicated that the histone modification mediated by HDACi treatment has an effect of promoting diversification of the antibody genes in treated animals. The nanoliposomes as a carrier of HDACi helped to increase the utilization rate of the loaded drugs, reduce the total dosage of administrations, and extend the time interval between administrations, making the drug feasible for newborn animals. This new method enables animals to generate antibodies against different epitopes of weakly immunogenic antigens, such as peptide antigens, and makes it possible to select antibodies with better performance for subsequent application in diagnostic reagents.
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ACS Applied Bio Materials
·
2026
·
IF 5.6
Jiajia Ren,Jie Liu,Yingying Wei,Jinglei Du,Lin Chen,Yongzhen Yang,Xuguang Liu,Shiping Yu,Yong Chen
Early diagnosis and precise treatment of liver cancer represent critical directions in medical research. Theranostic strategies integrating bioimaging and drug delivery have demonstrated significant potential, offering advantages such as high precision, enhanced imaging clarity, and improved therapeutic efficacy. Room temperature phosphorescence carbon dots@silica composites (RTP CDs@SiO2) stand out in bioimaging for their excellent properties such as long phosphorescence lifetime and low toxicity. However, their irregular morphology and large particle size restrict applications in drug delivery. In this study, RTP CDs@SiO2 composites were synthesized in an an aqueous solution via three approaches with different alkaline additives: no alkaline additive, ammonia solution, and arginine. Through the synergistic effect of the guanidine group, amino group, and carboxyl group in arginine molecules, nanoparticles with an average particle size of 14.2 nm, a phosphorescence lifetime of 1398.91 ms, and a visible afterglow duration of 12 s were prepared. The resultant composites possessed favorable biocompatibility with low toxicity, as well as outstanding phosphorescence imaging performance both in vitro and in vivo. The RTP CDs drug delivery system (CDs@SiO2-HA-DOX) was further constructed by loading the targeting molecule hyaluronic acid (HA) and the chemotherapy drug doxorubicin (DOX) via electrostatic adsorption and covalent coupling using CDs@SiO2 as a carrier. Results demonstrated that this system achieved a maximum drug loading capacity of 49.39% and exhibited pH-dependent DOX release under weakly acidic conditions. In vitro antitumor experiments revealed that CDs@SiO2-HA-DOX reduced the viability of HepG2 cells to 22.95%. In vivo studies using tumor-bearing mouse models confirmed a tumor inhibition rate of 75.48%, demonstrating significant antitumor efficacy. This work represents the first successful application of RTP CD composites in drug delivery. The developed system shows great promise as a theranostic candidate for liver cancer by integrating drug delivery and RTP imaging, highlighting its significant potential for cancer therapy applications.
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BMC MICROBIOLOGY
·
2026
·
IF 5.4
Ru Yi,Yu Ying,Liu Chuanxin,Liu Yunge,Liu Dongxia,Chen Ruyan,Chen Xiaowan,Bai Xiaoyang,Jiang Tao,Duan Jiajia
Podophyllotoxin (PPT) is a naturally occurring plant-derived insecticide, yet its potent cytotoxicity raises concerns regarding potential neurobehavioral toxicity in non-target organisms. However, the underlying mechanisms remain largely unclear. This study aims to investigate the neurotoxic effects of PPT in zebrafish. Three-day post-fertilization wild-type AB strain zebrafish were exposed to six concentration gradients of PPT (0.997–31.2 µg/mL). Based on the determined LC10 and MNLC values, four concentrations including 0.140, 0.420, 1.26, and 1.57 µg/mL were selected for intestinal-brain toxicity assessments and color preference tests were conducted. Histopathological damage in brain and intestinal tissues were evaluated through hematoxylin-eosin and acridine orange staining techniques. Subsequently, based on the maximum non-lethal concentration of 1.26 µg/mL, the mechanisms were further investigated using 16S rRNA sequencing, HM700 targeted metabolomics, and bulk RNA-seq, with key genes being validated via quantitative PCR. Correlation analysis was employed to elucidate the relationships among gut microbiota, differential metabolites, and gene expression. Our results showed that zebrafish exposed to various concentrations of PPT for 2 days exhibited significant intestinal and neurotoxic effects, including alterations in color preference behavior and structural damage in both the brain and intestinal tissues. 16S rRNA sequencing revealed reduced gut microbial diversity and increased levels of Acinetobacter and Perlucidibaca . Metabolomic profiling revealed that PPT disrupts purine metabolism, significantly reducing neuroprotective metabolites like adenosine and inosine, while increasing neurotoxic compounds such as quinolinic and oxalic acids. Transcriptomic analysis showed that genes associated with neuronal damage and inflammation were affected. qPCR confirmed that PPT exposure suppresses genes crucial for synaptic function (gad1b, drd2b) and upregulates genes related to the complement pathway (c3a.1, c1qc) and pro-inflammatory cytokines (IL-6, IL-1β, TNF-α). Correlation analysis linked changes in gut bacteria, Acinetobacter and Perlucidibaca , to altered purine metabolism and genes involved in synaptic and inflammatory pathways. These findings suggest that PPT-induced neurotoxicity is associated with alterations in gut microbial composition, dysregulation of purine metabolism, and changes in neuroinflammatory and synaptic-related pathways. This study highlights the potential association between microbiota-gut-brain axis disturbances and PPT-induced neurotoxicity and provides new insights for evaluating its environmental health risks. Graphical The alternative text for this image may have been generated using AI.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2026
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IF 6.8
Qiong Hu,Yuanquan Ran,Guo Chen,Wenwei Xia,Lanlan Yang,Junlin Liu,Bing Yang,Wenyu Wu,Dongxin Tang,Minyi Tian
Ethnopharmacological relevance Lemmaphyllum drymoglossoides (Baker) Ching is a widely used folk herbal remedy that has been employed in cancer treatment. However, studies on the anti-colorectal properties and phytochemicals of L. drymoglossoides essential oil (EO) are currently lacking. Aim of the study The purpose of this study was to examine the in vivo and in vitro anti-colorectal cancer properties of L. drymoglossoides EO and to examine its chemical composition. Materials and methods Using a Clevenger-type apparatus, L. drymoglossoides was extracted via hydrodistillation to obtain L. drymoglossoides EO. The cytotoxicity of L. drymoglossoides EO was evaluated using the MTT assay against the colorectal cancer HCT-116 cell line and the non-cancer NCM460 and L929 cell lines. Clone formation assays were employed to detect the effects of L. drymoglossoides EO on HCT-116 cell proliferation, and its effect on the cell cycle was assessed using flow cytometry. Moreover, EO's ability to induce apoptosis was evaluated using Hoechst 33258 staining, Annexin V-APC/PI staining, AO/EB staining, and morphological analysis. We evaluated the inhibitory activity of L. drymoglossoides EO against HCT-116 cell metastasis using Transwell migration and invasion detections and wound healing assays. Furthermore, western blotting was utilized to evaluate its impact on the expression of proteins related to cell proliferation, apoptosis, and metastasis. To further verify its in vivo anticancer effect, a nude mouse model of HCT-116 cell xenograft tumors was established. We analyzed the in vivo anticancer effect of L. drymoglossoides EO (100 mg/kg, intraperitoneal injection) by measuring tumor volume and weight, calculating tumor inhibition rate, and performing H&E and TUNEL staining. We employed an Agilent GC-MS/FID to perform chemical composition detection of L . drymoglossoides EO. Results L. drymoglossoides EO displayed selective cytotoxic activity with stronger cytotoxicity toward the HCT-116 cancer cell line (IC 50 : 34.44 ± 1.15 μg/mL) and lower cytotoxicity against the non-cancer cell lines NCM460 (IC 50 : 70.37 ± 1.39 μg/mL) and L929 (IC 50 : 85.32 ± 3.70 μg/mL). L. drymoglossoides EO induced G1 phase arrest by increasing p21 expression and decreasing Cyclin E1, CDK2, Cyclin D3, and CDK4 expression, thereby suppressing cell proliferation. Additionally, L. drymoglossoides EO elevated the Bax/Bcl-2 ratio, decreased ΔΨm, facilitated the release of Cyt c , and stimulated Caspase 9 and Caspase 3 activation, resulting in PARP cleavage, and triggering apoptosis by regulating mitochondrial apoptosis-related markers. L. drymoglossoides EO downregulated N-cadherin and upregulated E-cadherin, thereby restricting cell metastasis. L. drymoglossoides EO effectively inhibited the growth of HCT-116 xenograft tumors by inducing apoptosis in nude mice. Furthermore, GC-MS/FID analysis identified the major components of L. drymoglossoides EO as 1-hexanol, hexanal, linalool, hexanal dihexyl acetal, pimaradiene, trans -2-hexenal, trans - β -ionone, α -terpineol, and 3-hexenol. Conclusions L. drymoglossoides EO possesses promising anti-colorectal properties and holds development potential in the pharmaceutical field.
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ELECTROCHIMICA ACTA
·
2026
·
IF 5.8
Wenna Zhang,Zeguang Fang,Tong Yang,Zhiwen Han,Minglei Cao,Chuankun Zhang,Jian Li,Xiaobin Liao,Yan Zhao
● Non-flammability fluorinated electrolytes boost antioxidant activity and LMBs safety. ● Fluorinated electrolytes weaken the coordination between Li + and dipole. ● Advanced solvation structures promote ideal interfacial chemistry. ● The electrolyte is compatible with both Li metal anode and high-voltage cathode. Lithium metal batteries (LMBs) operated with high-voltage Ni-rich cathodes suffer from interphase fragility, electrolyte depletion, and safety concerns, which are tightly coupled to Li⁺ solvation chemistry. Here, guided by DFT screening and molecular dynamics analysis, we develop a nonflammable all-fluorinated electrolyte by co-solubilizing fluoroethylene carbonate (FEC) and bis(2,2,2-trifluoroethyl) carbonate (TFEC) at a 1:1 vol ratio. The relevant electrocatalyst weakens Li⁺-dipole interactions, reduces the solvent coordination number, and increases PF₆⁻ participation in the first solvation shell, thereby enabling anion-involved interfacial reactions. As a result, the relevant electrocatalyst with a tailored solvation configuration contributes to highly reversible Li plating/stripping with an average Coulombic efficiency of 98.4% over 300 cycles in Li||Cu cells and stable Li||Li cycling for over 900 h at 1 mA cm⁻ 2 . More importantly, Li||NCM811 full cells exhibit markedly improved high-voltage durability, retaining 60% of the initial capacity after 300 cycles at 4.3 V and 58% after 300 cycles at 4.6 V, with discharge capacities of 170.1 and 148.4 mAh g⁻¹ at 4 and 5 C, respectively. This work establishes a solvation structure-driven design principle for safe, high-voltage LMBs. The addition of TFEC solvent effectively adjusts the solvation configuration of the electrolyte and enhances the safety of Li metal batteries, further improving their high-voltage performance. Download: Download high-res image (232KB) Download: Download full-size image
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Nature Communications
·
2026
·
IF 18.1
Xuelian Wang,Hanyu Ma,Yongqiang Li,Liangfeng Chen,Caichao Ye,Yuhao Zhao,Hang Wang,Wanting Fu,Chen Yu,Fei Wang,Siwei Yang,Yuli Kang,Guqiao Ding,Zhen Li
Integrating the diagnostic and therapeutic functions of drugs poses challenges due to specific structural design requirements. However, the trial-and-error dilemma in performance-driven structural evolution of nanomaterials is unsatisfactory. Here, we propose a strategy using graphene quantum dots with sp 2 – sp 3 hybridized carbon frameworks for visualized, intelligent targeted clearance of metabolically reprogrammed senescent cells. We establish a structure-activity relationship between the complex nanostructures and photochemical reactivity. We show that property descriptor-based machine learning promotes the evolution of carbon nanostructures, endowing them with exceptionally high photodynamic efficiency. These machine-learning results also guide the design of carbon-based photo/electrocatalytic structures. By examining the interfacial transport properties of in situ carbon nanostructures in excited states, we identify changes in fluorescence and photodynamic activity of C 3 N quantum dots within metabolically reprogrammed cellular microenvironments. This allows fluorescence detection technology for senescent cells based on C 3 N quantum dots and an intelligent targeted clearance treatment plan. Designing complex carbon frameworks for specific applications often relies on trial-and-error. Here, authors integrate machine learning and interfacial mechanisms to evolve graphene quantum dot carbon frameworks, enabling selective detection and clearance of metabolically reprogrammed senescent cells.
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Nutrients
·
2026
·Vol. 18
·期 12
·2021
·
IF 5.8
Xiaojing Liu,Fei Pan,Yandan Wang,Wei Wei,Jun Jin,Xingguo Wang,Zhe Cui
Background/Objectives: Controlled comparisons of extracellular β-hydroxybutyrate (β-HB) responses induced by individual dietary fatty acids (FAs) remain limited. This study established a preliminary hepatocyte-derived in vitro assay for comparing FA-associated β-HB responses and used exploratory descriptor-based modeling for hypothesis-generating FA ranking. Methods: Dose- and time-dependent extracellular β-HB accumulation induced by nineteen dietary FAs was quantified in HepG2 and AML12 cells under nutrient-deprived assay conditions. For exploratory descriptor-based modeling, the 19-FA HepG2 dataset was split into 15 training compounds and four held-out compounds. Model stability was further assessed using repeated random splits, LOOCV, permutation testing, MCFA sensitivity analyses, and simple structural baseline models. Four additional structurally related FAs were tested only for preliminary experimental ranking assessment, not as an independent external test set. Results: C8:0 and C10:0 consistently induced the highest extracellular β-HB accumulation, whereas most long-chain saturated FAs and very-long-chain monounsaturated FAs showed lower responses. The single four-compound held-out subset yielded an apparent R2of 0.875, but repeated random-split assessment showed substantial split-dependent variability, and simple baseline models performed similarly to GradientBoosting. Conclusions: This study provides a preliminary in vitro dataset for comparing extracellular β-HB responses to selected dietary FAs under defined nutrient-deprived hepatocyte assay conditions. The descriptor-based analysis should be interpreted only as a small-sample, exploratory, hypothesis-generating structure–response framework.
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Medicine in Novel Technology and Devices
·
2026
·
IF 4.5
Yuqi Li,Jiawei Wang,Dongyan Li,Min Zhang,Lizhen Wang,Yubo Fan
Poly(glycerol dodecanoate) (PGD) is a biodegradable shape-memory elastomer that can be rendered magnetically responsive by incorporating Fe 3 O 4 . However, the effects of Fe 3 O 4 loading on degradation and biosafety remain unclear, which limits the in vivo translation of PGD/Fe 3 O 4 composites. This study aimed to clarify the relationship between Fe 3 O 4 content, degradation behavior, and gastrointestinal biosafety, thereby informing the design of PGD/Fe 3 O 4 devices. Five formulations containing 0, 5, 10, 20, or 40 wt% Fe 3 O 4 were prepared and designated as ION-0, ION-5, ION-10, ION-20, and ION-40. The composites were evaluated by FTIR, SEM/EDS, lipase-mediated degradation under dynamic and static conditions, Caco-2 assays, and 70-day oral exposure in mice. FTIR confirmed Fe 3 O 4 incorporation without altering the principal polyester backbone. Under both degradation conditions, the degradation percentage and medium acidification decreased with increasing Fe 3 O 4 content. Conditioned degradation media from ION-0, ION-5, ION-10, and ION-20 preserved Caco-2 viability and proliferation and maintained ZO-1 and Occludin expression, whereas ION-40 reduced junctional marker expression despite limited overt cytotoxicity. After daily gavage for 70 days, no obvious pathological lesions were observed in the heart, liver, spleen, lung, or kidney in any group, except for the intestine, where evident ileal injury and decreased ileal ZO-1 and Occludin expression were observed in the ION-40 group, whereas the other groups remained well tolerated. Overall, increasing Fe 3 O 4 loading improved structural persistence during degradation but narrowed the biosafety window. Within the present formulation set, composites containing up to 20 wt% Fe 3 O 4 provided the most favorable balance between degradation behavior and intestinal biosafety, supporting their further development for biomedical devices.
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