MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Chen Yiting,Gong Chunhui,Chen Kaiwei,Wang Ziwei,He Manli,Wang Peng,Chen Kai,Jiao Yan,Yang Yi
Strontium-90 ( 90 Sr) is a major radioactive component that has attracted great attention, but its detection remains challenging since there are no specific energy rays indicative of its presence. Herein, a biosensor that is capable of rapidly detecting Sr 2+ ions is demonstrated. Simple colorimetric method for sensitive detection of Sr 2+ with the help of single-stranded DNA was developed by preparing MnO 2 nanorods as oxidase mimic catalysis 3,3′,5,5′-tetramethylbenzidine (TMB). Under weakly acidic conditions, MnO 2 exhibited a strong oxidase-mimicking activity to oxidize colorless TMB into blue oxidation products (oxTMB) with discernible absorbance signals. Nevertheless, the introduction of a guanine-rich DNA aptamer inhibited MnO 2 -mediated TMB oxidation and reduced oxTMB formation, resulting in blue fading and diminished absorbance. Upon the addition of strontium ions to the system, the aptamers formed a stable G-quadruplex structure with strontium ions, thereby restoring the oxidase-mimicking activity of MnO 2 . Under the best experimental conditions, the absorbance exhibits a linear relationship with the Sr 2+ concentration within the range 0.01–200 μM, with a limit of detection of 0.0028 µM. When the concentration of Sr 2+ from 10 −8 to 10 −6 mol L −1 , a distinct color change gradient could be observed in paper-based sensor. We successfully applied this approach to determine Sr 2+ in natural water samples, obtaining recoveries ranging from 97.6 to 103% with a relative standard deviation of less than 5%. By providing technical solutions for detection, our work contributed to the effective monitoring of transportation of radioactive Sr in the environment. Graphical abstract
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
·
IF 6.1
Danni Jiang,Yang Yang,Xiaojuan Han,Qianni Li,Yingxiu Ma,Jun Jiao,Lan Chao
Fluorene-9-bisphenol (BHPF), a widely recognized alternative to bisphenol A (BPA), has been increasingly used in a wide range of industries. However, despite numerous studies, the direct mechanism underlying its male reproductive toxicity remains poorly understood. Here, a BHPF-exposed mouse model was established to evaluate the effect of BHPF on male fertility. The results showed that BHPF exposure induced reproductive dysfunction, including testis damage, spermatogenesis impairment, steroid hormone disruption and sperm quality degradation. Mechanistically, over 90,000 differentially methylated regions were identified and increased global testicular methylation levels indicated that DNA methylation may be associated with BHPF-induced testicular damage. Transcriptome analysis revealed 221 up-regulated genes and 227 down-regulated genes in BHPF-exposed mice, mainly enriched in the steroid biosynthetic pathway. Further combined methylome and transcriptome analysis revealed the critical methylated genes potentially involved in lipid metabolism. The differentially expressed genes (Cyp4a10 and Rdh1) were further confirmed to correlate with impaired male mouse fertility. Taken together, the results of this study provide a better understanding of the molecular mechanisms of toxicity effects induced by BHPF exposure from the perspective of methylome and transcriptome.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Sun Rengang,Zhou Yadong,Fang Yunzhu,Qin Yirui,Zheng Yekai,Jiang Li
The harm and impact of the COVID-19 pandemic have highlighted the importance of fast, sensitive, and cost-effective virus detection methods. In this study, we developed a DNA aptamer sensor using nanoparticle-enhanced surface plasmon resonance imaging (SPRi) technology to achieve efficient labeling-free detection of SARS-CoV-2 S protein. We used the same DNA aptamer to modify the surface of the SPRi sensor chip and gold nanoparticles (AuNPs), respectively, for capturing target analytes and amplifying signals, achieving ideal results while greatly reducing costs and simplifying the preparation process. The SPRi sensing method exhibits a good linear relationship ( R 2 = 0.9926) in the concentration range of 1–20 nM before adding AuNPs to amplify the signal, with a limit of detection (LOD) of 0.32 nM. After amplifying the signal, there is a good linear relationship ( R 2 = 0.9829) between the concentration range of 25–1000 pM, with a LOD of 5.99 pM. The simulation results also verified the effectiveness of AuNPs in improving SPRi signal response. The SPRi sensor has the advantage of short detection time and can complete the detection within 10 min. In addition, the specificity and repeatability of this method can achieve excellent results. This is the first study to simultaneously capture a viral marker protein and amplify the signal using polyadenylic acid (polyA)–modified DNA aptamers on the SPR platform. This scheme can be used as a fast and inexpensive detection method for diagnosis at the point of care (POC) to combat current and future epidemics caused by the virus.
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ANALYST
·
2011
·Vol. 136
·期 19
·3973-3980
·
IF 3.913
Feng Gao,Peng Cui,Xiaoxiao Chen,Qingqing Ye,Maoguo Li,Lun Wang
A novel and efficient method to evaluate the DNA hybridization based on a fluorescence resonance energy transfer (FRET) system, with fluorescein isothiocyanate (FITC)-doped fluorescent silica nanoparticles (SiNPs) as donor and gold nanoparticles (AuNPs) as acceptor, has been reported. The strategy for specific DNA sequence detecting is based on DNA hybridization event, which is detected via excitation of SiNPs-oligonucleotide conjugates and energy transfer to AuNPs-oligonucleotide conjugates. The proximity required for FRET arises when the SiNPs-oligonucleotide conjugates hybridize with partly complementary AuNPs-oligonucleotide conjugates, resulting in the fluorescence quenching of donors, SiNPs-oligonucleotide conjugates, and the formation of a weakly fluorescent complex, SiNPs-dsDNA-AuNPs. Upon the addition of the target DNA sequence to SiNPs-dsDNA-AuNPs complex, the fluorescence restores (turn-on). Based on the restored fluorescence, a homogeneous assay for the target DNA is proposed. Our results have shown that the linear range for target DNA detection is 0–35.0 nM with a detection limit (3σ) of 3.0 picomole. Compared with FITC-dsDNA-AuNPs probe system, the sensitivity of the proposed probe system for target DNA detection is increased by a factor of 3.4-fold.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Yanmei Li,Junmei Nie,Xinyu Li,Ni Yan,Renjiang Kong,Jiaqi Huang,Yeyang Wu,Zhuofeng Li,Yingtao Zhong,Xin Chen,Hong Cheng
Stimulator of interferon genes (STING) plays an essential role in initiating the innate anti-tumor immunity, which could be activated by the cytosolic DNA but suppressed by the immunosuppressive cells. In this work, a self-delivery ternary immunostimulant (designated as ImmSt) is fabricated for colorectal cancer therapy with the immunological cascades of photodynamic DNA damage and T regulatory cell inhibition. Among which, the photosensitizer of Chlorin e6 (Ce6), the STING agonist of 5,6-dimethylxanthine-4-acetic acid (DMXAA) and the phosphatidylinositol 3-kinases (PI3Ks) inhibitor of ZSTK474 could self-assemble into uniform nanomedicine without drug excipient, which enhances the drug stability, bioavailability and pharmacological activities. Importantly, the photodynamic therapy (PDT) effect of ImmSt produces enormous reactive oxygen species to ablate tumor cells, and also triggers the release of cytosolic DNA to amplify the STING signal in combination with the STING agonist. Moreover, ImmSt is able to induce a systemic anti-tumor immunity by the cascade activation of T cells and the decrease of T regulatory cells. In vitro and in vivo results confirm that ImmSt can efficiently eradicate colorectal cancer without severe side effects, which may provide a sophisticated mechanism for immunotherapy in consideration of the immune activation and immunosuppressive factors.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Liu Liang,Xu Qingqing,Hao Shuai,Chen Yun
MicroRNAs (miRNAs) play a vital role in regulating gene expression and are associated with a variety of cancers, including breast cancer. Their distorted and unique expression is a potential marker in clinical diagnoses and prognoses. Thus, accurate determination of miRNA expression levels is a prerequisite for their applications. However, the assays currently available for miRNA detection typically require pre-enrichment, amplification and labeling steps, and most of the assays are only semi-quantitative. Therefore, we developed a quasi-direct liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based targeted proteomics approach to quantify target miRNA by innovatively converting the miRNA signal into the mass response of a reporter peptide via a covalently immobilized DNA-peptide probe. Specifically, the probe containing the targeted proteomics-selected substrate/reporter peptide, GDR AVQLGVDPFR/AVQLGVDPFR, and the DNA sequence complementary to the target miRNA (i.e., miR-21) was first immobilized on APMTS modified silica nanoparticles using PDITC. After the immobilized probe was recognized and hybridized with the target miRNA, the excess probe was degraded using MBN and followed by a trypsin digestion of the hybrids. The reporter peptide was released and quantified using LC-MS/MS. The obtained LOQ was 5 pM. Finally, the developed assay was used for the quantitative analysis of miR-21 in breast cells and tissue samples.
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Biosensors-Basel
·
2024
·Vol. 14
·期 3
·135
·
IF 4.9
Hui Chen,Zhiyuan Zhuang,Naihan Xu,Ying Feng,Kaixin Fang,Chunyan Tan,Ying Tan
The ongoing COVID-19 pandemic, driven by persistent SARS-CoV-2 transmission, threatens human health worldwide, underscoring the urgent need for an efficient, low-cost, rapid SARS-CoV-2 detection method. Herein, we developed a point-of-care SARS-CoV-2 detection method incorporating recombinase polymerase amplification (RPA) and DNA–protein crosslinking chemiluminescence (DPCL) (RPADPCL). RPADPCL involves the crosslinking of biotinylated double-stranded RPA DNA products with horseradish peroxidase (HRP)-labeled streptavidin (SA-HRP). Modified products are captured using SA-labeled magnetic beads, and then analyzed using a chemiluminescence detector and smartphone after the addition of a chemiluminescent substrate. Under optimal conditions, the RPADPCL limit of detection (LOD) was observed to be 6 copies (within the linear detection range of 1–300 copies) for a plasmid containing the SARS-CoV-2 N gene and 15 copies (within the linear range of 10–500 copies) for in vitro transcribed (IVT) SARS-CoV-2 RNA. The proposed method is convenient, specific, visually intuitive, easy to use, and does not require external excitation. The effective RPADPCL detection of SARS-CoV-2 in complex matrix systems was verified by testing simulated clinical samples containing 10% human saliva or a virus transfer medium (VTM) spiked with a plasmid containing a SARS-CoV-2 N gene sequence or SARS-CoV-2 IVT RNA. Consequently, this method has great potential for detecting targets in clinical samples.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Qin Ma,Yilong Tu,Wen Yun,Mingming Zhang
A self-driven and self-catalytic (SDSC) tripedal DNA nanomachine was developed for microRNA-21 (miR-21) detection. The microRNA could open one arm of tripedal DNA nanomachine to form DNAzyme with a nearby arm through the proximity effect. After DNAzyme’s cleavage, the exposed DNA arm region competed with the third arm and produced a DNA segment (sequence Q). The released sequence Q initiated the next SDSC cycle of tripedal DNA nanomachine. In the special DNA nanomachines design, the components with close spatial localization were constructed on a single nanostructure, which significantly increased local reactant concentrations and reaction rates. A dynamic correlation was obtained from 10 pM to 50 nM between fluorescence signal and miR-21 concentration. The effective concentration of reactant greatly increased, compared with the free diffusible reactants. Consequently, the incubation time was significantly shorted to 35 min. This strategy showed a promising potential in miRNA detection and disease diagnosis.
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Bioactive Materials
·
2025
·
IF 20.3
Tingting Gai,Hao Zhang,Yan Hu,Ruiyang Li,Jian Wang,Xiao Chen,Jianhua Wang,Zhenhua Chen,Yingying Jing,Chenglong Wang,Long Bai,Xiuhui Wang,Jiacan Su
Given the limitations of allogeneic and artificial bone grafts, bone organoids have attracted extensive attention for their physiological properties that closely resemble natural bone, offering great potential to bone reconstruction for critical-sized bone defects. Although early-stage bone organoids such as osteo-callus organoids and woven bone organoids have been reported, functional bone organoids with vascularization and mineralization are currently unavailable due to the lack of bone-mimicking matrix and dynamic culture systems suitable for the long-term cultivation of mature bone organoids. Herein, a novel engineered bionic matrix hydrogels with multifunctional components and double network structure are developed by incorporating calcium phosphate oligomers (CPO) into a combination of bone-derived decellularized extracellular matrix (ECM) and salmon-derived deoxyribonucleic acid (DNA) via photo-crosslinking and dynamic self-assembly strategies. This kind of bionic matrix hydrogels facilitate recruitment, proliferation, osteogenesis and angiogenesis of bone marrow mesenchymal stromal cells (BMSCs). More importantly, vascularized and mineralized bone organoids are sequentially constructed using BMSCs-loaded engineered bionic matrix hydrogels via in vitro dynamic culture and in vivo heterotopic ossification. Meanwhile, this kind of engineered bionic matrix are capable of achieving efficient bone repair for cranial defect. These findings suggest that engineered bionic matrix hydrogels combined with such dynamic culture system, providing a promising strategy for functional bone organoids construction.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jing Xu,Yinbing liu,Xinqi Luo,Yujin Li,Yiqing Xing,Ke-Jing Huang
Self-powered electrochemical biosensors based on enzyme biofuel cells (EBFC) are uniquely valuable in developing real-time and on-site detection of heavy metal ions. This work combines graphdiyne/graphene (GDY/GR) heterojunction with Deoxyribozyme (DNAzyme) trigger catalytic hairpin self-assembly (CHA) circuit. In addition, the smartphone is introduced to obtain the detection signals. The results show that the designed sensor platform can be used for single-tube ultrasensitive and visual lead ion detection with a wide linear range of 0.003–5000 nM and a low detection limit (LOD) of 0.005 nM. This design enriches the routine screening methods for heavy metal ions in food and environmental samples and introduces new idea for the development and application of self-powered electrochemical biosensors.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xudong Yang,Kun Wang,Yuang Zhong,Weining Cui,Xin Jia,Lijun Yin
In this study, ferulic acid (FA) was enzymatically grafted onto sugar beet pectin (SBP) via laccase catalysis. Structural characterization of sugar beet pectin-ferulic acid conjugates (SFC) with varying substitution degrees was performed using UV–vis, FT-IR, and NMR analyses. Mechanistic analyses revealed that phenolic hydroxyl groups (-OH) on FA reacted with carboxyl groups (-COOH) of SBP via enzymatic catalysis, forming covalent linkages. Additionally, amide bond formation between FA's carboxyl groups and amino groups (-NH₂) of pectin-associated proteins was observed. Compared to unmodified pectin, SFC exhibited reduced crystallinity, lower apparent viscosity, and smoother surfaces, whereas thermal stability and in vitro antioxidant capacity were markedly improved. Notably, SFC with high substitution (>159.60 ± 0.60 mg/g) effectively inhibited malondialdehyde (92.18 %) and peroxide (61.29 %) formation in linoleic acid oxidation systems, while demonstrating DNA (66.07 %) and protein (17.79 %) oxidation inhibition through reduced carbonyl generation. These findings highlight SFC's potential as a multifunctional antioxidant in food and pharmaceutical applications.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Qipeng Long,Yang Lin,Jiaqi Zhang,Hengchang Zang,Yan Lu,Yufei Wang,Shulong Jiang
DNA tetrahedron (DT) nanostructures have shown broad application prospects in disease diagnosis and treatment due to their structural programmability, nontoxicity, and efficient cellular uptake. Recently, their potential as ideal nanocarriers for synergistic anticancer drug delivery has been increasingly recognized. In this study, the first use of a DT nanostructure to co-deliver shikonin (SK) and CpG oligonucleotide to achieve chemoimmunotherapy of triple-negative breast cancer (TNBC) is reported. In this nanoplatform, SK serves as both a cytotoxic agent and an immunogenic cell death (ICD) inducer, while CpG-modified DT (CpG-DT) has dual functions of drug carrier and immune adjuvant, which can significantly enhance the ICD effect. CpG-DT/SK exhibits an enhanced water solubility and tumor-specific accumulation. The synergistic combination of SK and CpG further facilitated the maturation of dendritic cells and the subsequent infiltration of CD8+ and CD4+ T cells, thereby achieving effective inhibition of 4T1 tumor growth. This DT-based co-delivery nanoplatform provides a promising strategy for TNBC treatment with great potential for clinical translation.
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