TALANTA
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2026
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IF 6.7
Wei Zhang,Jinhui Wang,Jialu Xu,Yulong Xu,Haiyan Li,Yujun Jiang
The environmental persistence and inherent biodegradation resistance of pesticide residues pose a serious threat to ecosystems and human health, necessitating the development of efficient monitoring strategies. Herein, we decipher the fluorine-fluorine (F···F) interaction within an AIE-active, trifluoromethyl-functionalized covalent organic framework (PEFA-COF) and exploit this molecular recognition motif for the selective detection of trifluralin (TFR). Benefiting from abundant trifluoromethyl moieties and superior luminescence properties, the PEFA-COF enables specific TFR recognition through a synergistic interplay of electrostatic forces, hydrogen bonding, and F···F interactions. This recognition event triggers a pronounced fluorescence quenching response via the internal filtration effect (IFE), facilitating highly sensitive and selective TFR quantification. The constructed PEFA-COF-based fluorescent sensing platform was successfully validated in complex water and milk matrices, achieving an excellent fluorescence quenching coefficient and a detection limit as low as 0.06 μmol/L. Collectively, this work not only deciphers the critical role of F···F interaction in pesticide recognition but also advances the function-led design of AIE-active COFs toward practical and robust pesticide residue monitoring in food supply chains.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Qiao Zeng,Xu Chen,Xinghao Chen,Wenying Yu,Qiao Wang,Zhan Tang
Novel in situ Ru gel improved Ru solubility, bioavailability, and retention. • Ru gel enhanced aqueous humor exposure by 5.14-fold and extended ocular retention. • Ru gel promoted corneal epithelial migration under hyperosmotic stress. • Ru gel suppressed inflammation, exhibiting dual therapeutic effects. The local delivery of JAK inhibitors for dry eye disease (DED) remains challenging due to poor drug solubility and rapid ocular clearance. This study reports an ion-sensitive in situ gelling system for ruxolitinib (Ru) using polyvinylpyrrolidone-K12 and gellan gum to enhance solubility and ocular retention. The optimized Ru gel exhibited shear-thinning behavior and rapid ion-activated gelation. In vitro permeation studies indicated that the gel matrix did not impede Ru diffusion. In scratch assays and Transwell assays performed under hypertonic stress conditions, the Ru gel demonstrated a more pronounced pro-migratory effect on cells within the scratch assay. This observation may indicate variations in the gel matrix's mechanism of action across different cellular migration patterns. Pharmacokinetic evaluation in rabbits demonstrated a 5.14-fold increase in aqueous humor exposure (AUC) compared with Ru solution, without significantly altering T max , MRT, or t 1/2 , suggesting that enhanced efficacy stems from prolonged precorneal retention rather than improved corneal penetration. In a benzalkonium chloride-induced rat DED model, the Ru gel significantly restored tear secretion, prolonged tear film break-up time, and repaired corneal integrity to levels comparable to healthy controls. While blank gel provided physical protection and reduced pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) possibly through lubrication and barrier effects, Ru-loaded gel uniquely promoted histological recovery of corneal epithelium and conjunctival goblet cells. These findings demonstrate that the engineered Ru gel is a safe and potent therapeutic platform that enhances ocular exposure and mitigates inflammation through prolonged retention and pharmacological repair, offering a promising strategy for DED management. Download: Download high-res image (256KB) Download: Download full-size image
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JOURNAL OF MOLECULAR STRUCTURE
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2025
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IF 4.7
Chunxiang Wu,Yuze Li,Zhengli Liang,Xiao Jiang,Hui Fang,Lixiang Qin,Xingyuan Bai,Lehui Liu,Xingxing Jiang,Zheshuai Lin,Hongming Liu
Second harmonic generation (SHG) response, birefringence, and optical band gap are several critical parameters of nonlinear optical (NLO) crystals. However, due to the mutual influence and constraints among the fundamental properties of NLO crystals, simultaneously optimizing these performance parameters poses a significant challenge. Herein, lanthanide iodates crystallizing in centrosymmetric (CS) space group were selected as parents, by introducing [SO 4 ] group and water molecule to induce symmetry breaking, a series of mixed-anion lanthanide iodate-sulfate hydrates, RE(IO 3 )(SO 4 )(H 2 O) 2 ∙H 2 O (RE = Eu, Gd, Tb, Yb, Y), were obtained. Their structures changed from the CS space group of the parents to non-centrosymmetric (NCS) one ( P 2 1 2 1 2 1 ). These crystals exhibit strong SHG responses (0.79−1.21 × KH 2 PO 4 ), moderate birefringence (0.061−0.109), short UV transmission cutoff edges (∼240 nm), and wide optical bandgaps (4.24−4.32 eV), demonstrating their potential as NLO crystalline materials with excellent comprehensive properties. This work highlights a feasible strategy of introducing [SO 4 ] group and water molecule into CS iodates to construct NCS structures, thereby developing potential high-performance NLO crystal materials.
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PLANT BIOTECHNOLOGY JOURNAL
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2025
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IF 10.5
Shu-Yue Zhang,Jian-Qiang Kong
Summary OSW-1 is a cholestane saponin uniquely produced in Ornithogalum saundersiae and exhibits a potential antitumor activity. The enzymes responsible for OSW-1 biosynthesis in O. saundersiae , however, remain unclear. Herein, cholesterol was confirmed to be the precursor of OSW-1 using stable-isotope labeling method. Next, two cytochrome P450s, Os CYP90B94 and Os CYP90G11, and their partner cytochrome P450 reductase Os CPR2 were mined by comparable transcriptome analysis and demonstrated to be involved in OSW-1 biosynthesis. Specifically, Os CYP90B94 catalyses cholesterol to 22 R -hydroxycholesterol, which is then converted to 16 β -hydroxy-22-oxo-cholesterol, a precursor of OSW-1 by the second P450 O sCYP90G11 via two consecutive oxidation reactions. Unlike their homologues, Os CYP90G11 and Os CYP90B94 displayed broader catalytic promiscuity. Os CYP90B94 recognizes both cholesterol and 7 β -hydroxycholesterol, while Os CYP90G11 can react with cholesterol, 22 R -hydroxycholesterol and 16 β ,22 R -dihydroxycholesterol, suggesting their involvement in more than one pathway besides OSW-1 biosynthesis. This study lays a foundation for the complete characterization of OSW-1 biosynthesis.
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TOXICOLOGY IN VITRO
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2025
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IF 2.7
Pinzheng Li,Hengbin Zhang,Yingchun Chen,Shuanghui Lu,Huidi Jiang,Su Zeng,Hui Zhou
Oxoglaucine (OXO), an aporphine alkaloid derived from Corydalis yanhusuo , exhibits a broad spectrum of pharmacological activities, including antiviral, antifungal, antiplatelet, and anti-hepatic fibrosis effects, with particularly promising anticancer potential. In this study, we systematically investigated the hepatotoxicity profile and underlying mechanisms of OXO using in vitro models. Dose–response assays revealed that while OXO induced significant cytotoxicity in hepatocytes, primary hepatocytes exhibited markedly reduced sensitivity compared to hepatocellular carcinoma cells at therapeutically relevant concentrations. Mechanistic studies in mouse primary hepatocytes and human primary hepatocytes attributed this selective cytotoxicity to differential cytochrome P450 (CYP) enzyme expression. This finding was functionally validated by the observation that co-treatment with the broad-spectrum CYP inhibitor aminobenzotriazole (ABT) enhanced OXO-induced toxicity in mouse primary hepatocytes and human primary hepatocytes. Regarding its mechanism of toxicity, OXO induced marked cytotoxicity in the HepG2 cell line by triggering mitochondrial-mediated apoptosis, as evidenced by a decreased Bcl-2/Bax ratio, cytochrome C release, Caspase-3 activation, and S-phase cell cycle arrest. Collectively, our findings in HepG2 cells and primary hepatocytes elucidate the role of CYP-mediated metabolism in the selective cytotoxicity of OXO. These findings not only provide crucial mechanistic insights but also support the further development of OXO as a promising candidate for preclinical and clinical evaluation in hepatocellular carcinoma.
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JOURNAL OF CONTROLLED RELEASE
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2025
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IF 11.5
Peng Jiang,Yongnian Zeng,Wen Zhang,Jiapeng Lei,Xue Jiang,Wei Li
Reactive oxygen species (ROS)-based therapies have emerged as promising non-antibiotic strategies for treating infected wounds due to their broad-spectrum antimicrobial activity and low risk of resistance development. However, their clinical translation remains limited by low delivery efficiency, poor bacterial targeting, and uncontrolled post-treatment inflammation. To address these challenges, we reported a multifunctional bilayer microneedle patch (LFT/S-MN) designed for efficient bacterial eradication and enhanced wound healing. The patch integrates positively charged lactoferrin-based nanoparticles (LFT NPs) conjugated with a porphyrin sonosensitizer (TAPPFe) in the dissolvable base layer, and a pH-responsive tip layer loaded with the anti-inflammatory agent spermidine (SPD). Upon insertion into infected tissue, the released LFT NPs efficiently target and aggregate negatively charged bacteria, enabling robust ROS generation through synergistic chemodynamic and sonodynamic therapy (CDT/SDT) under ultrasound stimulation. Concurrently, the acidic wound microenvironment triggers the gradual degradation of the tip layer, allowing controlled SPD release for immunomodulation. In a murine P. aeruginosa -infected wound model, the LFT/S-MN patch significantly reduced bacterial load, suppressed inflammation, promoted collagen deposition and neovascularization, and accelerated wound closure. This work presents a promising non-antibiotic strategy for wound disinfection and regeneration through synergistic CDT/SDT and inflammation modulation.
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Regenerative Therapy
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2025
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IF 3.5
Lan Chen,Lifang Zhu,Yu Cao
Introduction Pine pollen polysaccharides (PPPS) has anti-inflammatory, immunomodulatory, anti-oxidant, hypoglycemic, and anti-bacterial properties. PPPS can accelerate wound healing in mouse cutaneous wounds, yet it is unclear whether PPPS can promote diabetic wound healing. Methods Fibroblasts, keratinocytes, and human umbilical vein endothelial cells (HUVECs) were stimulated with high glucose (HG) to mimic hyperglycemic environment. Cell viability, apoptosis, migration, and angiogenesis were assessed by cell counting, Western blot, transwell migration, and tube formation assays. Neutrophil extracellular traps (NETs) and macrophage polarization were analyzed by immunofluorescence and flow cytometry. Streptozotocin-induced diabetes mellitus (DM) mice were subjected to skin wounds and PPPS administration to validate the role of PPPS in diabetic wound healing. Results PPPS treatment impaired HG-induced viability reduction, apoptosis promotion, and migration repression of fibroblasts, keratinocytes, and HUVECs, accompanied by promotion of angiogenesis of HUVECs under HG stimulation. Specifically, PPPS treatment facilitated NET degradation and suppressed macrophage M1 polarization. Furthermore, PPPS accelerated diabetic wound healing in DM mice, along with decreased citrullinated H3 and PAD4 protein levels and elevated CD31 protein levels, suggesting that PPPS facilitated re-epithelialization and vascularization and reduced NETs. Conclusions PPPS accelerates diabetic wound healing via mediating NETs and the polarization of M1-type macrophages, providing new insights into the promoting role of PPPS in diabetic wound healing.
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PROGRESS IN ORGANIC COATINGS
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2025
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IF 7.3
Lingjing Meng,Yue Yin,Jie Li,Yonglong Zhang,Yunfei Cai,Feiyan Yang,Jinfang Wu,Fang Guo,Wenbo Wang
Polyaniline (PANI) is widely employed as a filler to boost the corrosion resistance of organic coatings, leveraging its notable passivation ability and redox properties. However, its efficacy in protecting against corrosion is mainly confined to small defects such as pinholes due to the specific redox characteristics of PANI and the counterions it releases, which restricts further improvements in anticorrosive performance. To extend the repair capability of PANI fibers to large damaged areas, we propose entrapping neutral corrosion inhibitors within the PANI fibers. This was achieved by preparing benzotriazole (BTA)-entrapped HCl or sulfosalicylic acid (SSA)-doped PANI fibers through a one-step chemical oxidative polymerization process. The effects of various counterions and neutral inhibitors on corrosion protection were explored. A comprehensive release experiment examined unwashed and washed BTA-entrapped HCl or SSA-doped PANI fibers, confirming their pH-responsive controlled release capabilities. The results indicated that incorporating these fibers into polyvinyl butyral (PVB) coatings significantly enhances anti-corrosive performance compared to coatings without entrapped neutral corrosion inhibitors. Specifically, the low frequency impedance magnitude for PANI-SSA-BTA/PVB and PANI-HCl-BTA/PVB coatings increased by sixfold and elevenfold, respectively, over the pure PVB coating. This enhanced performance is attributed to the synergistic effect of the passivation of PANI fibers and the controlled release of BTA. The development of neutral corrosion inhibitors entrapped within acid-doped PANI represents an innovative approach to creating high-performance anticorrosion coatings, holding significant potential for advancing applications in corrosion protection.
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LANGMUIR
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2025
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IF 3.9
Yan Tang,Xinfei Li,Jingxia Liu,Wei Qu,Jun Liu,Junhui Ma,Lei Wang,Mai Jiang,Fangzhou Zhao,Xiangyun Lin,Nan Ma,Wenting Zhao,Xuesong Li,Meiyu Shao,Wei Zhang
Ion and protein adsorption occurs on the implant surface upon contact with tissues. Increasing research efforts focus on modifying or constructing the physical and chemical properties of surfaces to enhance antifouling capabilities. However, conventional in vitro evaluation methods, such as artificial urine and protein immersion models, often fail to replicate the complex interfacial interactions occurring in vivo. In this study, we explored a rabbit bladder implantation model and a biolayer interferometry (BLI) model as in vivo and in vitro systems, respectively, to accurately assess protein and ion interactions on implant surfaces. A series of 2-(Methacryloyloxy)ethyl 2-(trimethylammonio)ethyl phosphate (MPC) surfaces with varying degrees of cross-linking were fabricated on BLI fiber optic sensors and ureteral stents using Atom Transfer Radical Polymerization (ATRP). Our observations revealed that increased cross-linking of MPC-modified surfaces correlated with reduced ion and protein adhesion in the bladder implantation model. A similar correlation was noted with the MPC-modified fiber optic sensors. However, an opposite phenomenon was observed in traditional in vitro methods. The agreement between the BLI and rabbit bladder implantation models indicates that the BLI method effectively reflects the in vivo responses of these surfaces, thereby providing a rapid and accurate in vitro model for assessing protein and ion adsorption on implant surfaces in vivo.
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WATER RESEARCH
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2025
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IF 12.4
Mengge Fan,Yangjian Zhou,Jialing Luo,Yanpeng Gao,Junlang Qiu,Xin Yang
With drinking water disinfection techniques evolving from chlorination/chloramination to advanced oxidation processes, the conundrum of disinfection byproducts (DBPs) formation that remains for decades is becoming more challenging. This study focuses on the formation of unknown halogenated coupling byproducts, an important class of DBPs neglected in previous investigations, from UV/Chlorine reaction of phenols in presence of halides. To prioritize the highly toxic compounds, the effect-directed analysis strategy involving nontargeted identification with high-resolution mass spectrometry was utilized. The presence of bromide was found to enhance the formation of known DBPs, total organic halogens, and the overall toxicity of treated water. In contrast, iodide at environmentally relevant concentration exhibited minimal effects on the DBP formation and toxicity. Assisted with density functional theory calculation, a series of halogenated coupling byproducts were identified in the reaction involving bromide. The formation mechanisms of halogenated coupling byproducts consist of halogen substitution, free-radical-mediated electron transfer, hydroxylation, among others. As indicated by computational toxicity evaluation, these halogenated coupling byproducts were surprisingly harmful in human thyroperoxidase activity inhibition and aquatic toxicity. This study provides new insights into the DBP formation during UV/Chlorine reaction, and the findings unveiled the emergence of highly toxic halogenated coupling byproducts especially in presence of halides.
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FOOD CHEMISTRY
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2025
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IF 9.8
Yuhong Li,Yixian Zhao,Caiyu Ge,Pengcheng Zhao,Junjie Fei,Yixi Xie
Sunset Yellow (SY), a widely utilized synthetic food colorant, poses potential health risks upon excessive consumption. This work reports an ultrasensitive electrochemical sensor for the quantification of SY in commercial beverages. A spherical hierarchical porous carbon composite embedded with FeCo alloy nanoparticles (FeCo@HPC) was synthesized via sequential hydrothermal carbonization and pyrolysis. Density functional theory (DFT) simulations confirm that FeCo bimetallic synergy enhances interfacial electron transfer kinetics, while the hierarchical porous architecture and nitrogen-doped defect sites cooperatively facilitate SY adsorption and electrocatalytic conversion. The sensor demonstrates a linear response across 0.005–6 μM SY, achieving a detection limit of 2.1 nM (S/N = 3) and sensitivity of 23.6 μA·μM −1 . Validation via high-performance liquid chromatography (HPLC) yielded recovery rates of 97.2–103.5 % in spiked beverage matrices. This study establishes a cost-effective analytical platform for food additive surveillance while elucidating design principles for bimetallic carbon-based electrocatalysts.
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ADVANCED FUNCTIONAL MATERIALS
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2025
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IF 19
Shaoyue Shuai,Feifei Peng,Yufei Zhao,Xianggui Kong,Wenying Shi,Mingfei Shao
The high exciton binding energy ( E b ) in symmetric organic photocatalysts has long hindered solar-to-hydrogen efficiency. Here, a confinement-deconfinement engineering strategy is presented that transforms molecular symmetry from an intrinsic limitation into a tunable design parameter. By confining symmetric molecules within layered double hydroxide interlayers, a highly oriented stacking configuration is enforced, compelling molecules to deviate from their thermodynamically stable modes. Subsequent deconfinement preserves this metastable structure. This confinement-deconfinement synergy induces giant dipole moments (22.50 Debye) and internal electric fields, collectively reducing E b to 44.42 meV (comparable to inorganic semiconductors). Interfacial water molecules activated by deconfinement act as structural co-architects, further dynamically stabilizing the metastable supramolecular assemblies (SA) via hydrogen-bonding networks. The optimized SA achieves a hydrogen evolution rate of 100.48 mmol · g −1 · h −1 , representing the current state-of-the-art. These results establish confinement-deconfinement engineering as a universal paradigm for enhancing photocatalytic performance, as it enables controlled symmetry-to-function conversion across diverse symmetric molecular systems.
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