JOURNAL OF MEMBRANE SCIENCE
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2022
·
IF 10.53
Ke Chen,Linhan Ni,Hao Zhang,Chengming Xiao,Li Li,Xin Guo,Junwen Qi,Chaohai Wang,Xiuyun Sun,Jiansheng Li
Rational design of fillers and polymers is of great importance to fabricate mixed matrix membranes (MMMs) with high performance. In this work, fluorinated metal-organic frameworks (KAUST-7 (also referred as NbOFFIVE-1-Ni)) were adopted as nanofillers to prepare MMMs through physically blending into the polymer of intrinsic microporosity (PIM-1). Benefiting from the features of porous KAUST-7, including well CO 2 affinity, distinctive pore characteristics (size, shape, and functionality) and strong intermolecular interactions with PIM-1, the achieved MMMs demonstrated increased CO 2 /CH 4 separation performance and improved anti-physical aging property. Specifically, with the assistance of KAUST-7, the CO 2 permeability and CO 2 /CH 4 (30:70 vol%) selectivity of the optimized MMMs increased up to 5928 Barrer and 23.5, corresponding to improvements of 50% of and 88% of the permeability and selectivity compared with the pristine membranes, respectively. Importantly, 100-h continuous separation test results confirmed the satisfying long-term stability of the prepared MMMs. Notably, the MMMs maintained 91% of the initial CO 2 permeability after 150 days (decreased only 539 Barrer), implying that the CO 2 permeability decrement is about 5-fold lower than that of the control PIM-1 membrane. These results suggest that the fabricated MMMs are promising candidates for CO 2 separation in practical applications.
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JOURNAL OF NANOBIOTECHNOLOGY
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2022
·Vol. 20
·期 1
·1-16
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IF 9.429
Huang Haoming,Hu Dian,Chen Zhuo,Xu Jiarong,Xu Rengui,Gong Yusheng,Fang Zhengming,Wang Ting,Chen Wei
Background Type 1 diabetes mellitus (T1DM) is an autoimmune disease mediated by autoreactive T cells and dominated by Th1 response polarization. Insulin replacement therapy faces great challenges to this autoimmune disease, requiring highly frequent daily administration. Intriguingly, the progression of T1DM has proven to be prevented or attenuated by helminth infection or worm antigens for a relatively long term. However, the inevitable problems of low safety and poor compliance arise from infection with live worms or direct injection of antigens. Microneedles would be a promising candidate for local delivery of intact antigens, thus providing an opportunity for the clinical immunotherapy of parasitic products. Methods We developed a Schistosoma japonicum- egg tip-loaded asymmetric microneedle patch (STAMP) system, which serves as a new strategy to combat TIDM. In order to improve retention time and reduce contamination risk, a specific imperfection was introduced on the STAMP (asymmetric structure), which allows the tip to quickly separate from the base layer, improving reaction time and patient’s comfort. After loading Schistosoma japonicum- egg as the immune regulator, the effects of STAMP on blood glucose control and pancreatic pathological progression improvement were evaluated in vivo. Meanwhile, the immunoregulatory mechanism and biosafety of STAMP were confirmed by histopathology, qRT-PCR, ELISA and Flow cytometric analysis. Results Here, the newly developed STAMP was able to significantly reduce blood glucose and attenuate the pancreatic injury in T1DM mice independent of the adjuvants. The isolated Schistosoma japonicum- eggs micron slowly degraded in the skin and continuously released egg antigen for at least 2 weeks, ensuring localization and safety of antigen stimulation. This phenomenon should be attributed to the shift of Th2 immune response to reduce Th1 polarization. Conclusion Our results exhibited that STAMP could significantly regulate the blood glucose level and attenuate pancreatic pathological injury in T1DM mice by balancing the Th1/Th2 immune responses, which is independent of adjuvants. This technology opens a new window for the application of parasite products in clinical immunotherapy.
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Nature Biomedical Engineering
·
2021
·Vol. 6
·期 1
·32-43
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IF 25.671
Zheng Di-Wei,Deng Wei-Wei,Song Wen-Fang,Wu Cong-Cong,Liu Jie,Hong Sheng,Zhuang Ze-Nan,Cheng Han,Sun Zhi-Jun,Zhang Xian-Zheng
Because a host’s immune system is affected by host–microbiota interactions, means of modulating the microbiota could be leveraged to augment the effectiveness of cancer therapies. Here we report that patients with oral squamous cell carcinoma (OSCC) whose tumours contained higher levels of bacteria of the genus Peptostreptococcus had higher probability of long-term survival. We then show that in mice with murine OSCC tumours injected with oral microbiota from patients with OSCCs, antitumour responses were enhanced by the subcutaneous delivery of an adhesive hydrogel incorporating silver nanoparticles (which inhibited the growth of bacteria competing with Peptostreptococcus ) alongside the intratumoural delivery of the bacterium P. anaerobius (which upregulated the levels of Peptostreptococcus ). We also show that in mice with subcutaneous or orthotopic murine OSCC tumours, combination therapy with the two components (nanoparticle-incorporating hydrogel and exogenous P. anaerobius ) synergized with checkpoint inhibition with programmed death-1. Our findings suggest that biomaterials can be designed to modulate human microbiota to augment antitumour immune responses.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2021
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IF 7.312
Shujuan Yang,Rong Tang,Yanan Dai,Tianhao Wang,Zhinong Zeng,Liping Zhang
To expand the application of cellulose acetate (CA) membranes in wastewater treatment, the excellent ultrafiltration performance and anti-fouling properties should be achieved. Firstly, HKUST-1@ lignocellulose nanofibrils (LCNFs) was fabricated through green and in-situ strategy successfully, which combine the characteristics of the HKUST-1 and LCNFs. Then, the blended ultrafiltration membranes with different loading of HKUST-1@LCNFs (or HKUST-1, LCNFs) were prepared by the process of phase separation, which exhibit improved pore structure, hydrophilicity, and rejection rate. Excitedly, the highest pure water permeability of 207.32 L·m −2 ·h −1 and the flux recovery ratio (FRR) of 90.56% were obtained with M6 membrane (0.55 wt% HKUST-1@LCNFs), which was better than that of M2 (HKUST-1) and M3 (LCNFs). It is confirmed that HKUST-1 could inhibit the accumulation of LCNFs, making it taking advantages of its hydrophilic groups. Moreover, composite membranes have attractive antibacterial properties, and >90% of E. coli were killed by M7. This work demonstrated that CA membranes blended with HKUST-1@LCNFs possesses great potential in the wastewater treatment process.
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JOURNAL OF MOLECULAR LIQUIDS
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2021
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IF 6.165
Mingcan Mei,Xutao Hu,Zhen Song,Lifang Chen,Liyuan Deng,Zhiwen Qi
Although chemisorptive ionic liquids (ILs) are widely expected as promising CO 2 absorbents, rigorous thermodynamic analyses for such systems remain challenging due to the inevitable co-existence of chemisorption and physisorption. In this work, an experimental approach combining quantitative NMR (qNMR) spectroscopy and mass balance is proposed to straightforwardly quantify the chemisorption and physisorption of CO 2 in chemisorptive absorbent, and 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) is selected for an exemplary study. The total CO 2 solubility in [Emim][OAc] is measured by the pressure drop method, of which the chemisorption part is quantified by qNMR and the physisorption part is then determined by mass balance. Correspondingly, a large set of CO 2 solubilities at four temperatures (298.15 K, 313.15 K, 323.15 K, and 348.15 K) covering the low pressure range below 100 kPa are determined, where the chemisorption and physisorption contribution are distinguished quantitatively. Following that, thermodynamic analyses for the CO 2 absorption by [Emim][OAc] are made from chemisorption and physisorption point of view, respectively.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2021
·Vol. 168
·期 5
·057509
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IF 4.316
Yuan Li,Yutong Li,Chao Du,Pengju Zhang,Peipei Cen,Xinsheng Liu,Yonghong Li
Urinary 1-hydroxypyrene (1-OHP) is generally used as an exposure biomarker of polycyclic aromatic hydrocarbons (PAHs). In this work, a colloidal gold modified zeolitic imidazolate frameworks-67/carbon nanofiber paste electrode (Au@ZIF-67/CNFPE) was fabricated for the rapid and sensitive detection of 1-OHP. The morphologies of carbon nanofibers (CNF), ZIF-67, colloidal gold and colloidal gold@ZIF-67 (Au@ZIF-67) were studied by transmission electron microscopy (TEM). The electrochemical behavior of 1-OHP was investigated by square wave voltammetry (SWV). The effect factors like pH value, amount of colloidal gold, accumulation potential and accumulation time have been optimized. Under the optimal conditions, the constructed sensor responded to 1-OHP linearly in the concentration range of 0.0005–10 μM, and the detection limit was 0.1 nM. The reproducibility, stability and selectivity of this sensor were also examined. Finally, the electrode was successfully applied for the analysis of 1-OHP in human urine samples.
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ANALYTICAL CHEMISTRY
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2021
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IF 6.986
Xiaolun Peng,Yijia Wang,Wei Wen,Miao-Miao Chen,Xiuhua Zhang,Shengfu Wang
Programmed cell death protein 1 (PD-1) is one of the coinhibitory checkpoints upon T cell activation, the abnormal expression of which severely threatens host immune modulatation for chronic infection. Thus, fast and sensitive monitoring of PD-1 is of vital importance for early diagnosis and cancer treatment. The current detection methods largely based on enzyme-linked immunosorbent assay (ELISA) require time-consuming incubation and complicated washing steps. Herein, we designed a simple and portable nanofiber paper (NFP)-based fluorescence “off-on” immunosensor for PD-1 rapid determination. Molybdenum disulfide (MoS2) nanosheets modified NFP (MoS2–NFP) was employed for adsorbing and immobilizing CdSe/ZnS quantum dots-antibody (QDs-Ab) complex to construct a ready-to-use fluorescent immunosensor. The fluorescent signal of QDs-Ab was initially quenched by MoS2 under the Förster resonance energy transfer (FRET) effect. When the PD-1 target was specifically captured onto NFP by immunization, the QDs-Ab-PD-1 complex was promptly desorbed from the MoS2–NFP surface, resulting in FRET impediment and fluorescence recovery. As an alternative quenching agent, graphene oxide (GO) served as a contrast to investigate NFP-based sensing performance. Owing to superior quenching and desorption efficiency, the MoS2–NFP-based fluorescence immunosensor exhibited nearly 2-fold lower detection limit (85.5 pg/mL) than GO–NFP-based sensor (151 pg/mL) for PD-1 monitoring. Excellent selectivity and satisfactory recovery in PD-1 mouse cell culture supernatant samples were confirmed as well. In addition, the comparable detectability of the MoS2–NFP-based immunosensor was accurately evaluated by a standard PD-1 mouse ELISA kit. This study displayed a simple, rapid, low-cost, and portable point-of-care PD-1 assay, indicating its broad application prospect toward clinical diagnoses.
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BIOMATERIALS
·
2021
·
IF 12.479
Xueqing Zhou,Xuan Liu,Xiqin Yang,Li Wang,Yiling Hong,Keke Lian,Guoxi Qiu,Xuwei Shang,Zhongjun Ma,Hong Yuan,Fuqiang Hu
Tumor-associated macrophages (TAMs) in the tumor microenvironment (TME) play an important role in the development of tumors by secreting a variety of cytokines or directly communicating with tumor cells, making TAMs-targeted therapeutic strategies very attractive. It has been reported that oncogene c-Myc is related to every aspect of the oncogenic process of tumor cells and the alternative activation of macrophages. Hence, we constructed a glycolipid nanocarrier containing ROS-responsive peroxalate linkages (CSOPOSA) for ROS-triggered release of drugs and further modified it with Ex 26 (Ex 26-CSOPOSA), a selective sphingosine 1-phosphate receptor 1 (S1PR1) antagonist, to achieve the dual-targeted delivery of the c-Myc inhibitor JQ1 via S1PR1, which is overexpressed on both tumor cells and TAMs, thereby inducing apoptosis of tumor cells, and blocking M2 polarization of macrophages. More strikingly, our studies found that JQ1 could effectively inhibit the migration of tumor cells induced by M2 macrophages-derived exosomes via blocking Caveolin-1 related intercellular exosome exchange through lncRNA H19 and miR-107. The in vivo results revealed that this dual-targeted delivery strategy effectively inhibited tumor growth and metastasis with less systemic toxicity, providing a potential method for effective tumor treatment.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 8
·1-11
·
IF 5.833
Feng Jun,Lu Hao,Yang Yu,Huang Wenyi,Cheng Hao,Kong Hongxing,Li
Lijun
By in situ synthesis of gold nanoparticles (AuNPs) within the acid-etched (AE) MIL-101 (Cr) framework, AE-MIL-101 (Cr) nanocomposites embedded with AuNPs (AuNP/AE-MIL-101 (Cr)) were prepared as surface-enhanced Raman scattering (SERS) substrate. AuNPs are uniformly distributed and stabilized inside the metal-organic framework (MOF), thus forming more SERS hotspots. The SERS performance of AuNP/AE-MIL-101 (Cr) was evaluated using 4-mercaptophenylboronic acid (4-MPBA), 4-mercaptobenzoic acid (4-MBA), benzidine, and rhodamine 6G (R6G). The SERS substrate displays satisfying stability with very low background signal. When benzidine is used as the Raman reporter, the limit of detection (LOD) can reach 6.7 × 10 −13 mol·L −1 , and the relative standard deviation (RSD) of the intra- and inter-batch repetitive tests is less than 5.2%. On this basis, we developed a method for the detection of human carboxylesterase 1 (hCE 1) in human serum using AuNP/AE-MIL-101 (Cr) nanocomposite as SERS substrate and enzyme-linked immunosorbent assay (ELISA) colorimetric substrate as SERS marker. This method was used to determine hCE 1 in clinical serum samples without complicated sample pretreatment, and the detection results were consistent with the data determined by ELISA. In the concentration range 0.1–120 ng·mL −1 , the SERS signal intensity of benzidine at 1609 cm −1 gradually decreases with the increase of hCE 1 concentration (R 2 = 0.9948). The average recoveries of hCE 1 in human serum are in the range 84 to 108%, with RSDs lower than 7.7%. Graphical abstract
By using AuNP/acid etching-MIL-101(Cr) metal organic framework (MOF) as SERS substrate and enzyme-linked immunosorbent assay (ELISA) colorimetric substrate as the SERS marker, a rapid and sensitive method for the determination of human carboxylesterase 1 (hCE1) in human serum samples has been developed.
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CHEMICO-BIOLOGICAL INTERACTIONS
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2021
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IF 5.194
Min Wang,Longji Fang,Tongtong Liu,Xuejie Chen,Yan Zheng,Yilong Zhang,Shiming Chen,Zeng Li
The development of multi-target-directed ligands (MTDLs) may improve complex central nervous system diseases such as Alzheimer's disease (AD). Here, a series of 7-O-1, 2, 3-triazole hesperetin derivatives was evaluated for their inhibition of cholinesterase , anti-neuroinflammatory, and neuroprotective activity. Among the hesperetin derivatives, compound a8 (7-O-((1-(3-chlorobenzyl)-1H-1,2,3-triazol-4-yl)methyl)hesperetin) possessed excellent anti-butyrylcholinesterase activity (IC 50 = 3.08 ± 0.29 μM) and exhibited good anti-neuroinflammatory activity (IC 50 = 2.91 ± 0.47 μM) against NO production through remarkably blocking the NF-κB signaling pathway and inhibiting the phosphorylation of P65. In addition, a8 showed a remarkable neuroprotective effect and lacked neurotoxicity up to 50 μM concentration. Furthermore, possessing significant self-mediated Aβ 1-42 aggregation inhibitory activity, chelated biometals and reduced ROS production were found in compound a8 . In the bi-directional transport assay, a8 exhibited a blood–brain barrier penetrating ability. In this study, the Morris water maze task showed that compound a8 significantly improved the learning and memory impairment of the scopolamine-induced AD mice model. Results highlighted the potential of compound a8 to be a potential MTDL for the development of anti-AD agents.
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ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Kemin Lv,Zhimeng Yu,Marcelo M. Pedroso,Bin Wu,Zhen Gao,Bingfang He,Gerhard Schenk
Establishing a suitable immobilization strategy to improve the accessibility of immobilized cellulase for insoluble cellulose and subsequently recover the enzyme from the remaining substrate is crucial to promote the industrialization of biofuels. Here, using iminodiacetic acid (IDA) and Ni2+ ions, a novel metal-chelated copolymer of methacrylic acid and methyl methacrylate was prepared for the immobilization of His-tagged processive endoglucanase EG5C-1 via affinity interaction between polyhistidine tag and Ni2+. The optimum loading capacity of the functionalized polymer (Eud-IDA-Ni2+) for EG5C-1 was about 280 mg/g, where more than 80% of the activity was recovered. Immobilized EG5C-1 exhibited improved thermal and pH stability and better reusability than the free one, and after five cycles of usage, the hydrolysis productivity remained above 70% of the initial value. The Eud-IDA-Ni2+/EG5C-1 biocomposite displayed reversibly soluble–insoluble characteristics with pH change, which was in the soluble state during the enzyme reaction process but could be recovered in an insoluble form by lowering the pH after the reaction. Thus, the yield obtained from the hydrolysis of an insoluble phosphoric acid-swollen cellulose substrate was similar for the free and immobilized form of EG5C-1. Our combined results suggested that a metal-ion-chelated, pH-responsive polymer had the potential for His-tagged cellulase immobilization to improve both the operational efficiency and economic benefit of the cellulosic biorefinery industry.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Qihang Yu,Wu Tang,Yang Hu,Jian Gao,Ming Wang,Sihong Liu,Huanhuan Lai,Liang Xu,Cong Fan
For the first time, two novel air-sensitive Li-rich organic cathodes ( m -TP4OLi and p -TP4OLi) are designed and reported. The structure-performance relationship for the two organic cathodes is comparatively studied. Using organic anode of lithium terephthalate (Li 2 TP), the resulting Li 2 TP II p -TP4OLi all-organic Li-ion batteries can deliver the peak discharge capacity of 330 mAh g −1 cathode in 1–3.4 V, corresponding to the high energy density of ~574 Wh kg −1 cathode using the median voltage of 1.74 V. Furthermore, the fabricated graphite II p -TP4OLi Li-ion batteries can show the peak discharge capacity of 305 mAh g −1 cathode in 1.5–3.4 V with a high median voltage of 2.4 V, resulting in the peak energy density of ~732 Wh kg −1 cathode (2.4 V × 305 mAh g −1 ). Meanwhile, the graphite II p -TP4OLi Li-ion batteries exhibit impressive device stability over 20,000 cycles. To the best of our knowledge, the peak energy density of our p -TP4OLi is currently the world record among Li-rich organic cathodes in lab conditions, outperforms the energy density of inorganic LiFePO 4 (~476 Wh kg −1 ), comparable with that of inorganic LiCoO 2 (~760 Wh kg -1 ) under the same lab conditions.
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危险品化学品经营许可证（带存储）