INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Hongdong Song,Aijing He,Xiao Guan,Zhengyu Chen,Yize Bao,Kai Huang
Epigallocatechin-3-gallate (EGCG) has gained appreciable attention because of its health benefits. However, the poor permeability across the intestine limits its use. In this study, we have fabricated chitosan-coated EGCG-hordein nanoparticles (Cs-EHNs), with the aim to enhance the intestinal permeability of EGCG. Cs-EHNs were fabricated by layer-by-layer electrostatic stacking method, and its uptake and transcellular permeability were studied in the Caco-2/HT29 co-culture model. The constructed Cs-EHNs had the average diameter of 296 nm, polymer dispersity index (PDI) of 0.30, zeta potential of 59.6 mV, and showed a spherical morphology. Encapsulation efficiency of EGCG was 87.3%. The transcellular permeability experiments indicated that the apparent permeability coefficient (P app ) of Cs-EHNs was higher than that of free EGCG. Furthermore, the cellular uptake of Cs-EHNs was studied by specific endocytosis inhibitors, and results showed that the uptake mechanisms of Cs-EHNs were through caveolae-mediated endocytosis and macropinocytosis. This study demonstrated that encapsulation of EGCG using chitosan-coated hordein nanoparticles could be a promising approach to improve the absorption of EGCG.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Zhijuan Li,Minghao Hou,Baoye Yao,Chuhan Ma,Shitong Zhang,Honglei An,Yichen Liu,Haibao Duan,Tongfei Li,Yawen Tang
The development of high-entropy alloy (HEA) nanocatalysts with ultrasmall size and hollow structures is vital yet challenging for efficient hydrogen evolution reaction (HER) across all pH conditions. Herein, we present a mild and facile hydrothermal strategy for synthesizing hollow PtRuRhCoCu HEA nanospheres (NSs) via self-assembly of sub-3 nm alloy nanoparticles directed by N , N ′-methylenebisacrylamide (MBAA). The resulting PtRuRhCoCu HEA features a multielement composition, interconnected hollow architecture, and well-dispersed ultrafine particles, providing abundant active sites and strong synergistic electronic interactions. As a result, the PtRuRhCoCu HEA catalyst achieves remarkable HER activity with low overpotentials of 10 mV (1 M KOH), 15 mV (0.5 M H 2 SO 4 ) and 30 mV (1 M phosphate-buffered saline, PBS) at 10 mA cm −2 , outperforming commercial Pt black. Moreover, this catalyst also exhibits outstanding long-term stability across a broad pH range, operating continuously for 80 h under alkaline conditions at various current densities without noticeable degradation. The density functional theory (DFT) calculations confirm that this HEA exhibits optimized hydrogen adsorption energy and electronic structure. When integrated into a full electrolyzer, it delivers a cell voltage of 1.48 V at 10 mA cm −2 , demonstrating great potential for water splitting. This work presents an effective design strategy for next-generation HEA electrocatalysts toward green hydrogen production.
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DOI
PubMed
高影响因子
Bioengineered
·
2021
·
IF 3.269
Na Li,Xueyuan Guo,Rui Li,Jian Zhou,Fangfang Yu,Xianliang Yan
Macrophage infiltration is a hallmark pathological change observed in early stage myocardial ischemia/reperfusion (MI/R) injury and one of the main causes of myocardial damage. Here, we investigated the effects of p-Coumaric acid (p-CA) on macrophage polarization following MI/R injury and its mechanisms. In vitro, p-CA decreases the expression of LPS/IFN-γ-induced M1 macrophage markers (TNF-α, IL-6, iNOS and CCL2) and increases IL-4-induced M2 macrophage markers (IL-10, CD206, Arg1 and Mrc) in mouse bone marrow-derived macrophages (BMDMs). Additionally, p-CA elevated indoleamine 2, 3-dioxygenase (IDO) protein expression levels, M2 macrophage polarization and M2 macrophage markers through IL-4. In contrast, repression of IDO attenuated p-CA functions regulating BMDMs through IL-4. In vivo, IDO expression was downregulated in mouse hearts subjected to MI/R injury. Treatment of p-CA increased IDO expression in the hearts of MI/R mice. Functionally, p-CA decreases M1 macrophage markers, the number of M1 macrophages and inflammation around heart tissue following MI/R injury. Importantly, p-CA reduces cardiomyocyte apoptosis caused by MI/R. Altogether, our study identified that p-CA modulates macrophage polarization by promoting IDO expression and that p-CA attenuates macrophage-mediated inflammation following MI/R by promoting M2 macrophage polarization through IDO.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-20
·
IF 9.429
Deng Han,Shang Wenting,Wang Kun,Guo Kunxiong,Liu Yu,Tian Jie,Fang Chihua
Despite advancements in diagnostic methods and therapeutic strategies, the mortality rate of hepatocellular carcinoma (HCC) remains as high as its incidence rate. Most liver cancers are detected in the advanced stages, when treatment options are limited. Small HCC is difficult to diagnose and is often overlooked by current imaging methods because of the complexity of the liver environment, especially in cirrhotic livers. In the present study, we developed a tumor “cruise missile”, mesoporous Fe 3 O 4 -containing glucose oxidase-conjugated GPC3 peptide nanoparticles (FGP NPs). It was designed to enhance the accuracy of small HCC visualization to 85.7% using combined ultrasound/photoacoustic imaging in complex liver environment, which facilitated sequential catalytic targeted therapy for small HCC. In a carcinogen-induced mouse HCC model, FGP NPs could be used to accurately diagnose HCC in a liver cirrhosis background as well as distinguish HCC nodules from other abnormal liver nodules, such as cirrhosis nodules and necrotic nodules, by dynamic contrast-enhanced photoacoustic imaging. In a mouse xenograft HCC model, highly reactive oxygen species were formed by sequential catalytic reactions, which promoted HCC cell apoptosis, significantly increasing the survival of the model mice. The present study provides a basis for the precise detection and elimination of small HCCs in the complex liver environment. Graphical Abstract
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高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2013
·
IF 3.499
Dacheng Fan,Weizhi Sun,Peiju Qiu,Zhiyong Wu,Yantuan Li,Shengbiao Wan,Tao Jiang,Lijuan Zhang
We synthesized a series of novel 3-indolyl cyclopent[ b ]indoles by trifluoroacetic acid mediated cyclodimerizations . The reaction showed high stereoselectivity and moderate to good yields. The influencing factors for stereoselectivity were systematically analyzed and a stepwise reaction mechanism was proposed. The cell viability tests in two colon and two lung cancer cell lines indicated the 1-benzyl-2-phenyl-group in 3-indolyl cyclopent[ b ]indoles was critical for the observed lower IC 50 s in these compounds. Western blot analysis demonstrated that the compound inhibited the expression and phosphorylation of EGFR through altered HSP90 expression. Further cell cycle and cell cycle check point protein analyses showed expected anti-cellular proliferation and cell cycle arresting properties associated with suppressed EGFR expression and phosphorylation. These data revealed a novel molecular mechanism explaining the observed cytotoxicities for these compounds.
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高影响因子
Journal of Functional Foods
·
2022
·
IF 5.223
Xiang Li,Yunhui Zhang,Congying Zhao,Bowei Zhang,Bo Peng,Yan Zhang,Jin Wang,Shuo Wang
Bisphenol A (BPA), a plasticizer, is widely believed to cause metabolic disorders. However, an effective intervention is still absent. This study investigated the beneficial effects of Epigallocatechin-3-gallate (EGCG) on insulin resistance and microbial disturbance induced by BPA. The serum glucose, insulin, total triglyceride, and total cholesterol were decreased in BPA-treated mice after the intervention of EGCG. The sensitivity of insulin signals in peripheral tissue was improved by modulating the phosphorylation of IRS-1, Akt, and GSK-3β. In addition, 16S rRNA analysis for fecal microbiota showed the relative abundance of Ruminiclostridium , Blautia , unidentified_Ruminococcaceae , and Candidatus_Saccharimonas were restored by the high dose of EGCG. Furthermore, EGCG increased the concentrations of fecal short-chain fatty acids and protected the gut barrier impaired by BPA. Overall, our results demonstrated the positive effects of EGCG on BPA-induced insulin resistance and gut microbial dysbiosis. The current study provides a potential treatment for the chronic metabolic toxicity of BPA.
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高影响因子
MOLECULES
·
2019
·Vol. 24
·期 15
·2769
·
IF 3.06
Xican Li,Xiaojian Ouyang,Minshi Liang,Dongfeng Chen
The biological process, 3-O-galactosylation, is important in plant cells. To understand the mechanism of the reduction of flavonol antioxidative activity by 3-O-galactosylation, myricetin-3-O-galactoside (M3OGa) and myricetin aglycone were each incubated with 2 mol α,α-diphenyl-β-picrylhydrazyl radical (DPPH•) and subsequently comparatively analyzed for radical adduct formation (RAF) products using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS) technology. The analyses revealed that M3OGa afforded an M3OGa–DPPH adduct (m/z873.1573) and an M3OGa–M3OGa dimer (m/z958.1620). Similarly, myricetin yielded a myricetin–DPPH adduct (m/z711.1039) and a myricetin–myricetin dimer (m/z634.0544). Subsequently, M3OGa and myricetin were compared using three redox-dependent antioxidant analyses, including DPPH•-trapping analysis, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO•)-trapping analysis, and•O2inhibition analysis. In the three analyses, M3OGa always possessed higher IC50values than those of myricetin. Conclusively, M3OGa and its myricetin aglycone could trap the free radical via a chain reaction comprising of a propagation step and a termination step. At the propagation step, both M3OGa and myricetin could trap radicals through redox-dependent antioxidant pathways. The 3-O-galactosylation process, however, could limit these pathways; thus, M3OGa is an inferior antioxidant compared to its myricetin aglycone. Nevertheless, 3-O-galactosylation has a negligible effect on the termination step. This 3-O-galactosylation effect has provided novel evidence that the difference in the antioxidative activities of phytophenols exists at the propagation step rather than the termination step.
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高影响因子
CHEMISTRY & BIODIVERSITY
·
2020
·Vol. 17
·期 12
·e2000763
·
IF 2.039
Zhiyan Liu,Jiangping Cao,Xiaoting Yan,Wanqing Cheng,Xiaoguang Wang,Ruige Yang,Yong Guo
Many phytopathogenic fungi cause severe damage to crop yields. In continuation of our research aimed at the discovery and development of natural products-based fungicides, a series of thirty-one sarisan attached 3-phenylisoxazolines were synthesized and evaluated for their antifungal activities against five phytopathogenic fungi ( B. cinerea , C. lagenarium , A. solani , F. solani , and F. graminearum ). Among all title sarisan derivatives, compounds IV2 , IV14 and IV23 showed potent antifungal activity against some phytopathogenic fungi. In particular, compound IV2 exhibited a broad-spectrum and more potent antifungal activity against A. solani , F. solani , and F. graminearum than the commercial fungicide Hymexazol. In addition, compounds IV2 , IV14 and IV23 also displayed relative low toxicity on normal NRK-52E cells. This work will give some insights into the development of sarisan derivatives as new fungicide candidates in plant protection. Graphical
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高影响因子
Journal of Energy Storage
·
2024
·
IF 8.9
Mengyue Liu,Kexin Wan,Xinmiao Zhang,Mingkun Wang,Dawei Yang,Jishi Wei,Kun Chang,Hongwei Tang
NASICON-type cathodes as promising candidates have attracted more and more attention, and Na 3 V 2 (PO 4 ) 3 is one of the outstanding representatives. In particular, it can tolerate complex interface reaction processes during the Zn 2+ insertion/desertion in the aqueous environment. However, existing three-dimensional channels in the intrinsic Na 3 V 2 (PO 4 ) 3 always hardly play to their advantages as narrow pathways and some other defects. This work has considered both the conductivity and capacity of this vanadium-based NASICON cathode at the same time, where a sodium-rich cathode material Na 3.2 V 1.8 Mn 0.15 Mg 0.05 (PO 4 ) 3 (NVMMP) with Mn 2+ and Mg 2+ dual-substituents has been designed considering the low cost and environmentally friendly characteristics. The synergistic effects introduced by Mn 2+ and Mg 2+ substitution led to a significant increase in the capacity and cycle life of the optimized NVMMP cathode material (with a capacity retention rate of 90.6 % after 2500 cycles at 2 A g −1 ). Furthermore, the reversible storage mechanism of Zn 2+ in NVMMP cathode materials and the theoretical study of such synergistic substitution on intrinsic electronic conductivity have been revealed by different in/ex-situ characterization techniques and theoretical calculations, where redox processes generated by Mn 3+ /Mn 4+ , enhanced ionic/electron pathway, and the pinning effect caused by injecting inert MgO 6 octahedra into the NASICON framework are key points to high capacity and long-life span.
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高影响因子
ELECTROCHIMICA ACTA
·
2018
·
IF 5.116
Junlong Niu,Shuai Chen,Wenna Zhang,Wenwen Zhang,Keke Chai,Guo Ye,Danqin Li,Weiqiang Zhou,Xuemin Duan,Jingkun Xu
Nanowire poly (3,4-dihydro-2 H -thieno [3,4- b ] [1,4]dioxepin-3-yl)methanol (PProDTM) free-supporting films with conductivities around 1.92 S cm −1 were prepared using a template-free electropolymerization method. The structure, morphology, mechanical properties, and thermal stability of the PProDTM films were characterized by Fourier transform infrared spectroscopy, UV–vis spectroscopy, scanning electron microscopy, dynamic mechanical analysis and thermogravimetric analysis . Moreover, the capacitance performances of PProDTM films were investigated in aqueous and organic electrolytes (acetonitrile (ACN) and dichloromethane (DCM)). In 0.1 M H 2 SO 4 , a symmetrical supercapacitor based on PProDTM exhibited specific capacitance of 89.2 F g −1 at a current density of 1.0 A g −1 , an energy density of 3.1 Wh kg −1 at power density of 0.5 kW kg −1 and the cycling capacitance retention was 74.8% after 5000 cycles. As the scan rate and current density increased, the specific capacitance of PProDTM films clearly decreased in DCM, but no obvious changes occurred in water and ACN. In ACN containing 0.1 M Bu 4 NBF 4 , a symmetrical supercapacitor based on PProDTM exhibited specific capacitance of 99.6 F g −1 at a current density of 1.0 A g −1 , an energy density of 3.5 Wh kg −1 at power density of 0.5 kW kg −1 and the cycling capacitance retention was 82.2% after 5000 cycles. These results imply that our flexible free-supporting PProDTM films are promising for future use in supercapacitors .
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高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
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2013
·
IF 2.823
Long-Hua Zhou,Na Wang,Wei Zhang,Zong-Bo Xie,Xiao-Qi Yu
An facile and green one-pot route has been developed for the synthesis of chromenes using salicylaldehyde and α,β-unsaturated ketones. α-Amylase from Bacillus subtilis shows excellent catalytic activity and exerts good adaptability to different substrates in the reaction. This promiscuous enzyme-catalyzed domino reaction not only extends the application of α-amylase from B. subtilis for new chemical transformations, but also provided an alternative synthetic method for 2 H -chromene derivatives.
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高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2014
·
IF 2.045
Mingjie Wang,Tianxiang Yin,Chen Xu,Zhiyun Chen,Weiguo Shen
The measurements of the liquid–liquid coexistence curve and the heat capacity for binary mixture {1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([C2mim][NTf2]) + 1-propanol} have been precisely performed. The values of the critical exponents α and β in the critical region were obtained and coincided well with the 3D-Ising ones. The complete scaling theory was applied to well represent the asymmetric behavior of the diameter of the coexistence curve, indicating an important role of the heat capacity related term in the complete scaling formulizm. A comparison of the reduced critical parameters to those predicted by the restricted primitive model clearly showed the solvophobic criticality of the studied system.
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高影响因子
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