MOLECULES
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2024
·Vol. 29
·期 9
·1993
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IF 4.2
Beilei Wang,Dezhi Yang,Xiaohong Zhong,Yuhui Liu,Yong Huang
Abnormal viscosity is closely related to the occurrence of many diseases, such as cancer. Therefore, real-time detection of changes in viscosity in living cells is of great importance. Fluorescent molecular rotors play a critical role in detecting changes in cellular viscosity. Developing red emission viscosity probes with large Stokes shifts and high sensitivity and specificity remains an urgent and important topic. Herein, a novel viscosity-sensitive fluorescent probe (TCF-VIS1) with a large stokes shift and red emission was prepared based on the 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) skeleton. Due to intramolecular rotation, the probe itself does not fluorescence at low viscosity. With the increase in viscosity, the rotation of TCF-VIS1 is limited, and its fluorescence is obviously enhanced. The probe has the advantages of simple preparation, large Stokes shift, good sensitivity and selectivity, and low cytotoxicity, which make it successfully used for viscosity detection in living cells. Moreover, TCF-VIS1 showed its potential for cancer diagnosis at the cell level and in tumor-bearing mice by detecting viscosity. Therefore, the probe is expected to enrich strategies for the detection of viscosity in biological systems and offer a potential tool for cancer diagnosis.
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CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Yuan Zhang,Dong Wang,Gaili Liu,Kun Wang,Shaomin Shuang,Chuan Dong
2-Mercaptobenzothiazole (MBT), commonly employed in the rubber and metal industries, poses a significant environmental hazard, detrimental effects on aquatic ecosystems and potential carcinogenicity in humans. Urgent measures are warranted to develop practical strategies for MBT analysis and mitigation. This study introduces a novel nanozyme sensor, orderly consistent with dendritic mesoporous silica nanoparticle (DMSN), CuO nanoparticle cargo, and MnO 2 shell (designated as DMC), engineered for both MBT detection and degradation. By harnessing oxidase-like MnO 2 and peroxidase-like CuO mediated singlet oxygen ( 1 O 2 ), superoxide anion radicals (O 2 •- ), and hydroxyl radicals (•OH) generation, combining with the MBT-triggered colorimetric change of 3,3′,5,5′-tetramethylbenzidine (TMB), the absorbance and colorimetric methods with a broad linear detection range spanning 0.25 to 30 μM and 122 nM limit of detection are established. Furthermore, leveraging the evident ROS production by the nanozymes, DMC exhibits the advanced capability to modulate MBT levels and notably mitigate its biotoxicity oxidatively due to the ultrahigh reactive •OH from CuO nanoparticle compare with the MnO 2 . This research presents a facile and cost-effective approach for MBT detection while contributing positively to MBT management and environmental preservation efforts.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xiayu Liu,Ying Shi,Donghong Liu,Qihe Chen
An exopolysaccharide (EPS)-producing bacterium, identified as Weissella cibaria based on 16S rDNA sequencing, was isolated from Chinese traditional rice wine koji. Whole-genome sequencing (WGS) revealed 2261 core genes and 3 plasmids, with 95 carbohydrate-active enzymes identified in the genome. The EPS produced by W. cibaria ZJU.1 was primarily composed of glucose, with a molecular weight of 144.5 kDa, as determined by high-performance anion-exchange chromatography (HPAEC) and high-performance gel chromatography (HPGPC). Structural analysis indicated that the EPS predominantly consists of α-(1→6) glycosidic bonds (88.32%) and α-(1→3) branches (11.68%). Microscopic examination revealed an interleaved fibrous structure at low concentrations and a lamellar structure at higher concentrations. Thermal analysis demonstrated good stability, with the carbon ring structure intact up to 264.12°C. Furthermore, the EPS exhibited excellent dispersion stability and rheological properties at higher concentrations. Activity prediction using a zebrafish model suggested potential therapeutic benefits, including alleviation of diabetes, anti-tumor, and anti-epileptic effects. This study highlights the promising food and pharmaceutical applications of W. cibaria ZJU.1 EPS.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2024
·
IF 1.2
Jiamei LI,An YAN,Mingquan WANG,Di LI
Targeted delivery of proteins into desired cell groups is crucial for disease therapy and cellular functionalization. However, current delivery methods face severe side effects and off-target problems, making it difficult to achieve cell-targeted protein delivery. Herein, we developed a user-friendly membrane fusion liposome for cancer cell-targeted protein delivery. Phosphorothioated DNA-mediated membrane fusion was employed as an efficient transmembrane delivery approach. The phosphorothioated DNA was capped with the AS1411 aptamer, which specifically recognizes nucleolar proteins on the cancer cell membrane, enabling a controllable delivery of proteins into targeted cancer cells. This delivery system exhibited commendable biocompatibility and targeted delivery ability, thereby realizing highly effective cancer cell inhibition in vitro. The in vivo results further suggested that the membrane protein-responsive membrane fusion delivery system offers a new avenue for highly biocompatible and targeted protein delivery.
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JOURNAL OF CONTROLLED RELEASE
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2025
·
IF 11.5
Liuxin Yang,Fengya Jing,Dandan Wei,Xiaocong Zhao,Yinghua Tao,Tao Liu,Tianzhu Zhang
Abdominal wall defects caused by trauma, congenital rupture, and intra-abdominal infection remain challenging due to the large wound area and complex complications. Herein, an assembled mesenchymal stem cell (MSCs)-laden granular hydrogel (termed assembled GSD@FPs), loaded with basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF), is developed. This hydrogel is constructed through dynamic covalent cross-linking ( via borate ester bonds) among dopamine-grafted gelatin methacrylamide (GelMA-DA), phenylborate-modified hyaluronic acid (HA-PBA), and epigallocatechin-3-gallate (EGCG), serving as multifunctional bulk building blocks for cell delivery and abdominal wall repair. The designed assembled granular hydrogels possess good rheological properties, self-healing, injectability, and tissue-adhesion properties. Detailed in vitro cell experiments are conducted, revealing that the GSD@FPs granular hydrogels can effectively promote cell proliferation, cell migration and angiogenesis. Furthermore, in abdominal wall defects, assembled GSD@FPs significantly accelerates the tissue healing process by simultaneously inhibiting the inflammatory response, promoting collagen deposition, and promoting cell proliferation and angiogenesis. Importantly, the assembled GSD@FPs granular hydrogels can also provide mechanical support and increase the thickness of regenerated tissue (1727.8 ± 169.6 μm for the control group, 3204.2 ± 278.5 μm for the assembled GSD@FPs group at 14 d). Eventually, the GSD granular hydrogels biodegraded, facilitating tissue remodeling and generating new muscle tissues. Therefore, this study provides a promising strategy with great potential for application in abdominal wall repair.
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BIOELECTROCHEMISTRY
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2011
·
IF 3.52
Xiao Liu,Liqiang Luo,Yaping Ding,Daixin Ye
A novel poly-glutamic acid (PGA) film modified carbon paste electrode (CPE) incorporating carbon nanotubes (CNTs) was first prepared for the determination of l -tryptophan ( l -Trp). Scanning electron microscopy and Fourier transform infrared spectroscopy were applied for characterization of the surface morphology of the modified electrodes and cyclic voltammetry was used to investigate the electrochemical properties of the proposed electrode towards the oxidation of l -Trp. Optimization of the experimental parameters was performed with regard to pH, ratio of CNTs, concentration of glutamic acid , electro-polymerization cycles, accumulation time and concentration of sodium dodecylbenzene sulfonate . The linearity between the oxidation peak current and the l -Trp concentration was obtained in the range of 5.0 × 10 − 8 to 1.0 × 10 − 4 M with a detection limit of 1.0 × 10 − 8 M ( S / N = 3) and the sensitivity was calculated to be 1143.79 μA∙mM − 1 ∙ cm − 2 . In addition, the PGA modified CPE incorporating CNTs displayed high selectivity, good stability and reproducibility, making it suitable for the routine analysis of l -Trp in clinical use.
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Advanced Healthcare Materials
·
2024
·
IF 10
Xiaochen Chen,Pengfei Tian,Wenwen Chai,Liyan Zhang,Muyan Qin,Mengke Fan,Na Liang,Jua Kim,Yansong Wang,Weijia William Lu,Deping Wang,Xu Cui,Haobo Pan
Tumor therapy has advanced significantly in recent years, but tumor cells can still evade and survive the treatment through various mechanisms. Notably, tumor cells use autophagy to sustain viability by removing impaired mitochondria and clearing excess reactive oxygen species (ROS). In this study, the aim is to amplify intracellular oxidative stress by inhibiting mitochondrial autophagic flux. Multisynergistic environmental-response nanoparticles (ERNs) are engineered by integrating gold nanoparticles and copper peroxide with borosilicate bioactive glass. The controlled release of copper and inhibition of autophagy flux triggered an overabundance and accumulation of oxidative stress within the tumor cells. This stress triggered immunogenic tumor cell death, believed to initiate a systemic immune response. The tumor microenvironment (TME) transitioned back to a normal physiological state as tumor cells are ablated. ERNs responded to the microenvironment changes by depositing hydroxyapatite on the surface and spontaneously enhancing bone regeneration. This innovative formulation facilitates the functional transition of ERNs from “anti-tumor therapy” to “biomineralization” that kills cancers and induces new bone formation. Overall, it is shown that the ERNs effectively eradicate cancers by utilizing chemodynamic therapy, starvation therapy, and immunotherapy.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xiaoqin Li,Xiaoxue Lu,Linyu Zhang,Zhixiong Cai,Dianping Tang,Wenqiang Lai
A colorimetric immunoassay was built for determination of carcinoembryonic antigen (CEA) based on papain-based colorimetric catalytic sensing system through the use of glucose oxidase (GOx). In the presence of GOx, glucose was catalytically oxidized to produce H 2 O 2 . Through the assistance of papain (as a peroxide mimetic enzyme), the signal came from the oxidative color development of 3,3',5,5'-tetramethylbenzidine (TMB, from colorless to blue) catalyzed by the generated H 2 O 2 . Herein, a sandwich-type immunoassay was built based on GOx as labels. As the concentration of CEA increased, more GOx-labeled antibodies specifically associate with target, which leaded to more H 2 O 2 generation. Immediately following this, more TMB were oxidized with the addition of papain. Accordingly, the absorbance increased further. As a result, the concentration of CEA is positively correlated with the change in absorbance of the solution. Under optimal conditions, the CEA concentration was linear in the range of 0.05–20.0 ng/mL, and the limit of detection (LOD) reached 37 pg/mL. The papain-based colorimetric immunoassay also exhibited satisfactory repeatability, stability, and selectivity.
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Journal of Materials Chemistry B
·
2024
·
IF 6.1
Dengyue Chen,Jieli Yin,Chen Liu,Jiaqi Guo,Mao Li,Baoyin Chen,Xuewen Zhang,Bing Wang,Xuan Zhu
Both chemodynamic therapy and photodynamic therapy, based on the production of reactive oxygen (ROS), have excellent potential in cancer therapy. However, the abnormal redox homeostasis in tumor cells, especially the overexpressed glutathione (GSH) could scavenge ROS and reduce the anti-tumor efficiency. Therefore, it is essential to develop a simple and effective tumor-specific drug delivery system for modulating the tumor microenvironment (TME) and achieving synergistic therapy at the tumor site. In this study, self-assembled nanoparticles (named CDZP NPs) were developed by copper ion (Cu2+), Doxorubicin (Dox), Zinc phthalocyanine (ZnPc) and a trace amount of poly (2-(di-methylamino) ethylmethacrylate)-poly [(R)-3-hydroxybutyrate]-poly (2-(dimethylamino) ethylmethacrylate (PDMAEMA-PHB-PDMAEMA) through chelation, π-π stacking and hydrophobic interaction. This triple factors-responsive (pH, laser and GSH) nanoparticles demonstrated unique advantages through the synergistic effect. Highly controllable drug release ensured its effectiveness at the tumor site, Dox-induced chemotherapy and ZnPc-mediated fluorescence (FL) imaging exhibited the distribution of nanoparticles. Meanwhile, Cu2+-mediated GSH-consumption not only reduced the intracellular ROS elimination but also produced Cu+ to catalyze hydrogen peroxide (H2O2) and generated hydroxyl radical (∙OH), thereby enhancing chemodynamic and photodynamic therapy. Herein, this study provided a green and relatively simple method for preparing multifunctional nanoparticles that can effectively modulate the TME and improve synergetic cancer therapy.
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RSC Advances
·
2017
·Vol. 7
·期 41
·25694-25701
·
IF 3.108
Xiaoju Shi,Guoyue Lv,Xiaodong Sun,Dianbo Cao,Guangyi Wang,Yulei Chang
Recently, several targeted drug delivery systems have shown promising antitumor activity towards different types of cancer. However, targeting the tumor microenvironment, cellular surface and/or only one organelle may not achieve the expected therapeutic effects. Herein, a targeted drug delivery with stepwise targeting and image-guided strategy was obtained for enhancing antitumor therapy and overcoming various problems caused by the random drug distribution of traditional medicine. In this design, a multifunctional nanoplatform, TPGS/PPG2L@IONPs@DOX/TPP–DOX, based on poly(ethylene glycol)-poly(ε-caprolactone)-poly(amidoamine)-lactose acid (mPEG-PCL-G2.0 PAMAM-LA, PPG2L) copolymer and D-α-tocopherol polyethylene glycol 1000 succinate (TPGS), as a co-carrier, incorporates iron oxide nanoparticles (IONPs) as imaging agents and loads a DOX/triphenylphosphonium (TPP)–DOX drug for chemotherapy. In this nanoplatform micelle, the LA as targeting moieties to hepatocarcinoma cells is the first targeting step. After the drug is released from the hybrid micelles, the incorporated DOX and TPP–DOX target delivery to nuclei and mitochondria for the next targeting step. Furthermore, with the help of MRI contrast agents of IONPs, the distribution of hybrid micelles was monitored. As a result, the hybrid micelles demonstrated enhanced antitumor cell activity in both HepG2 and HepG2/DOX cells. The results indicated that the multifunctional hybrid micelles showed great potential in enhancing antitumor activity under image-guided and stepwise targeted chemotherapy.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
·
IF 5.2
Wenjun Sun,Dehao He,Xueru Ren,Jiayin Li,Linghui Meng,Fang liu,Aiping Zheng
Understanding cellular uptake and intracellular dissolution of drug nanocrystals is necessary. Due to their superior sensitivity, luminescent materials with aggregation-induced emission (AIE) are embedded into drug nanocrystals (hybrid nanocrystals) as a good strategy for tracking intact nanocrystals in cells. However, AIE materials are strongly hydrophobic and tend to self-aggregate, generating fluorescent interference. To exclusively mark nanocrystals, AIE materials must be embedded in nanocrystals and remain soluble when released from dissolved nanocrystals. In this study, cyclosporin A (CsA) nanocrystals (CsA/THPE NCs) embedded with tetrakis(4-hydroxyphenyl)ethylene (THPE) possessing AIE properties were prepared. The self-fluorescence of CsA/THPE NCs was collectively corroborated by scanning electron microscopy, powder X-ray diffraction, fluorescence microscopy, and dissolution experiments. During the dissolution test, CsA/THPE NCs showed a certain degree of slow-release property. Cellular uptake studies were conducted with Caco-2 cells and characterized by confocal microscopy, flow cytometry, and quantitative analyses. The intact nanocrystals could be directly taken up by cells. The cellular uptake was found to be concentration- and time-dependent. Based on the above experimental results, hybrid nanocrystals integrated with AIE materials were verified to have specific self-fluorescence. Additionally, the dissolution rate of nanocrystals can be adjusted by physically embedding them with other materials.
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Frontiers in Pharmacology
·
2024
·
IF 4.4
Xu Xiaodong,Ding Zhao,Pu Chunlin,Kong Changchang,Chen Shijunyin,Lu Weiling,Zhang Jianfa
Introduction: Overexposure to ultraviolet (UV) light is known to cause damage to the skin, leading to sunburn and photo-aging. Chemical sunscreen products may give rise to health risks including phototoxicity, photosensitivity, and photosensitivity. Natural polysaccharides have attracted considerable interests due to diverse biological activities.Methods: A novel polysaccharide isolated was purified and structurally characterized using chemical methods followed by HPLC, GLC-MS, as well as 1D and 2D NMR spectroscopy. The photoprotective effect of the EPS on UVB-induced damage was assessed in vitro using cultured keratinocytes and in vivo using C57BL/6 mouse models.Results: The average molecular weight of the EPS was 5.48 × 106 Da, composed of glucose, mannose and galactose residues at a ratio of 2:2:1. The repeating units of the EPS were →3)-β-D-Glcp (1→3) [β-D-Galp (1→2)-α-D-Glcp (1→2)]-α-D-Manp (1→3)-α-D-Manp (1→. In cultured keratinocytes, the EPS reduced cytotoxicity and excessive ROS production induced by UVB irradiation. The EPS also exhibits an inhibitory effect on oxidative stress, inflammation, and collagen degradation found in the photodamage in mice. 1H NMR-based metabolomics analysis for skin suggested that the EPS partly reversed the shifts of metabolic profiles of the skin in UVB-exposed mice.Conclusion: The EPS exhibits skin photoprotective effects through regulating oxidative stress both in vivo and in vitro. Our findings highlight that the EPS is a potential candidate in sunscreen formulations for an efficient solution to UVB radiation.
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