BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Hong Zhang,Chenggang Zhang,Zhaotong Wang,Wenwu Cao,Miao Yu,Ye Sun
Sensitive and anti-interference detection of targeted signal(s) in body fluids is one of the paramount tasks in biosensing. Overcoming the complication and high cost of antibody/aptamer-modification, surface-enhanced Raman spectroscopy (SERS) based on antibody/aptamer-free (AAF) substrates has shown great promise, yet with rather limited detection sensitivity. Herein, we report ultrasensitive and anti-interference detection of SARS-CoV-2 spike protein in untreated saliva by an AAF SERS substrate, applying the evanescent field induced by the high-order waveguide modes of well-defined nanorods for SERS for the first time. A detection limit of 3.6 × 10 −17 M and 1.6 × 10 −16 M are obtained in phosphate buffered saline and untreated saliva, respectively; the detection limits are three orders of magnitude improved than the best records from AAF substrates. This work unlocks an exciting path to design AAF SERS substrates for ultrasensitive biosensing, not limited to detection of viral antigens.
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Materials Chemistry Frontiers
·
2023
·Vol. 7
·期 6
·1100-1109
·
IF 7
Suyang Li,Junjie Chen,Wanbing Xu,Biao Sun,Jiechen Wu,Qiang Chen,Pei Liang
Bimetallic gold core–silver shell (Au@Ag) surface-enhanced Raman scattering tags draw broad interest in the fields of biological and environmental analyses. Herein, an efficient hybrid microfluidic chip was designed to prepare uniform Au@Ag core–shell nanoparticles, and DTNB was used as the internal standard tag molecule to prepare Au@DTNB@Ag for SERS detection. Homogeneous core–shell nanoparticles with a particle size of 90 nm were prepared by mixing a silver precursor and a gold core in a microfluidic chip. The relative standard deviation (RSD) of the particle size distribution was close to 10%, and the detection limit of 4-MBA was as low as 10-10 M. In order to solve the influence of SERS signal fluctuation, a uniform Au@DTNB@Ag core–molecule–shell structure was synthesized in a microfluidic chip, and the characteristic peak of the analyte was corrected by the relative intensity of the DTNB characteristic peak (1335 cm−1). The experimental results showed that the SERS detection was achieved with high reproducibility, and the SERS peak intensity had a good linear correlation with the concentration. The homogeneous SERS substrate prepared using a microfluidic chip has potential for sensitive and reliable detection of environmental chemical contaminants.
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JOURNAL OF FOOD AND DRUG ANALYSIS
·
2022
·
IF 6.157
Wenwen Chen,Qiang Chen,Wei Zhang,De Zhang,Zhi Yu,Ying Song,Xiubing Zhang,Dejiang Ni,Pei Liang
The problem of aflatoxin contamination emerged gradually in the field of food safety. Surface-enhanced Raman spectroscopy (SERS) is an ultra-sensitive and non-destructive spectroscopy technology with extensive application prospects in the detection field. In this paper, with the detection of AFB1 as the target, Au@Ag NPs substrate with uniform morphology and strong SERS effect was prepared. Furthermore, the intermediates formed by hydrogen bonding between AFB1 and melamine facilitate the binding of toxin molecules to the substrate. Moreover, the AFB1-melamine-Au@Ag NPs detection system was established by optimizing the melamine dosage, and the limit of detection can reach 10 −8 mol/L (M). In this method, AFB1 (concentration range of 10 −4 M – 10 −7 M) in tea oil, the Raman signal intensity of AFB1 shows an excellent linear, logarithmic relationship, and the correlation coefficient is 0.9685. Therefore, this work has achieved simple, sensitive, and stable AFB1 detection and has broad application potential in food safety detection.
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Advanced Materials Technologies
·
2023
·Vol. 8
·期 17
·2300367
·
IF 6.8
Yongxing Lin,Pengfei Qi,Jincheng Liu,Yizhi Zhuo,Cheng Cai,Haibao Zhang,Xiangyang Li,Xianglan Liu,Lin Chen,Sihai Luo,Xingyou Tian
Large-area gold-coated polyamide 66 (PA66) nanopillar (PA66NP@Au) arrays consisting of secondary lamellar structures and sub-10–nm gaps are fabricated for surface-enhanced Raman spectroscopy (SERS) using ultrathin anodized aluminum oxide (AAO) template, solution-phase growth of PA66 lamellae, and Au sputtering. Such closely packed PA66NP@Au arrays with high density of electromagnetic field enhancement (“hot spots”) on large area exhibit high SERS sensitivity for detection of rhodamine 6g (R6G) molecules with concentration down to 10 −14 m and a large Raman analytical enhancement factor (AEF) of ≈10 10 . Furthermore, the as-fabricated PA66NP@Au arrays provide highly sensitive Raman signals in detection of a commonly pesticide residue, carbendazim with a limit of detection (LOD) of 0.05 mg L −1 (0.05 ppm), which is lower than that of maximum residue limit of 0.2 ppm allowed in Europe Union. The as-fabricated PA66NP@Au arrays are promising for highly sensitive and uniform SERS detection.
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Analytical Methods
·
2023
·Vol. 15
·期 37
·4842-4850
·
IF 3.1
Zhiyou Zeng,Xiaoyu Yang,Yongguo Cao,Sanshan Pu,Xinyu Zhou,Rongli Gu,Yiqian Zhang,Caijun Wu,Xiaojun Luo,Yi He
Nowadays, the presence of highly toxic and persistent residues of pesticides in water and food around the world is becoming a serious problem, and so their rapid and sensitive detection is critical to human health. In this work, a 3D composite nanoparticle of porous PDA (polydopamine) microspheres and Au NPs (PPDA@Au NPs) was proposed as a SERS substrate to detect pesticides. Porous PDA as a substrate was first synthesized with F127 (Pluronic F127), dopamine hydrochloride, and 1,3,5-TMB (1,3,5-trimethylbenzene) under weakly alkaline conditions by a one-step method. Then, HAuCl4 was in situ reduced in the pores of PPDA spheres and grew sequentially for effecting the reducibility of PPDA. As a result, uniform 3D PPDA@Au NPs with “hot spots” were successfully synthesized as SERS substrates, which could effectively avoid the agglomeration of gold nanoparticles to greatly improve the sensitivity and uniformity of the SERS platform. At the same time, methyl parathion, 4-chlorophenol, and 2,4-D as representatives of pesticides were detected with the proposed PPDA@Au NP-based SERS platform, with detection limits lower than 7.26, 7.52, and 11 ng mL−1, separately. The current work presents a simple preparation method to prepare sensitive and uniform SERS platform PPDA@Au NPs, which have potential for applications in actual pesticide and drug testing.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yan-ling Liu,Jian Zhu,Guo-jun Weng,Jian-jun Li,Jun-wu Zhao
Herein, we reported a robust and sensitive SERS sensing platform based on hollow porous pentagonal Au/Ag alloy nanotubes (Au/AgANTs) for simultaneous determination of three food contaminants. With the increase amount of etching agent, the morphology of the nanoparticles changed from solid to hollow porous structures , and then the disappearance of pores, accompanied by a trend of first increasing and then decreasing SERS activity. The Au/AgANTs with pentagonal cross section, hollow and pore structure provide stronger local electric field. Under optimized experimental conditions, they were utilized to achieve the simultaneous determination of rhodamine B (RhB), methylene blue (MB) and malachite green (MG) in shrimp paste with low detection limit (5.3 ×10 −11 M for RhB, 1.27 ×10 −10 M for MB and 4.07 ×10 −12 M for MG), wide linear detection range (1.0 ×10 −8 ∼1.0 ×10 −4 M for RhB, 1.0 ×10 −9 ∼1.0 ×10 −5 M for MB, and 1.0 ×10 −9 ∼1.0 ×10 −5 M for MG) and satisfactory recoveries (97.24–102.66%), suggesting that the developed method is a promising tool for accurate quantification of the three food contaminants at trace level.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-11
·
IF 6.408
Wang Mi,Shen Yanting,Hu Xiaoxiao,Zhu Yanyan,Wang Jing
Reactive oxygen species (ROS) are involved in drug-induced cytotoxicity by regulating cell signaling, inducing oxidative stress, and damaging the DNA and proteins. Examining ROS production in cells under the stimulation of chemotherapeutic drugs is of great importance for understanding the ROS roles and identifying the mechanism of drug-induced cytotoxicity. Here, a silver/gold (Ag/Au) nanoshell-based colorimetric and surface-enhanced Raman spectroscopy (SERS) dual-response nanoprobe was proposed for ROS sensing on the basis of Ag etching. In this study, as a kind of ROS, hydrogen peroxide (H 2 O 2 ) was detected by the prepared nanoprobe. The linear ranges of 0.5–100 μM with a limit of detection (LOD) of 0.343 μM for the colorimetric determination and 1–50 μM with LOD of 0.294 μM for SERS determination were achieved. The detection of cellular ROS concentration after stimulation by cisplatin, paclitaxel, doxorubicin, and 5-fluorouracil was validated by the nanoprobe. The nanoprobe could also be used to detect the signal pathway of ROS production by cisplatin stimulation. This study provided a simple and novel dual-response nanoplatform for detecting and monitoring ROS in cells, which holds great potential for elucidating the mechanism of occurrence and treatment of ROS-involved diseases. Graphical abstract
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
·
IF 4.831
Borong Yu,Yue Mao,Jiangli Li,Jiaosuo Wang,Binbin Zhou,Pan Li,Yuanyuan Ma,Zhangang Han
Surface enhanced Raman spectroscopy (SERS) is a promising technique for trace determination. More and more attention is focused on hybrid SERS substrates, which coupled with noble metal nanoparticles and carbon-based materials. Herein, expanded graphite (EG) is used to prepare EG-covered support by ultrasonic washing and filtration. Such support is flexible and can be cut into any shape. And the contact angle ( θe ) for Au nanorods (Au NRs) sol on the EG-covered support was 108.2° and the hydrophobic surface is helpful for Au NRs to construct ‘hot spots’ during evaporation. The limits of detection (LOD) for crystal violet (CV), thiram, malachite green (MG) and methylene blue (MB) were as low as 1 ppb, 50 ppb, 1 ppb and 1 ppb, respectively. Moreover, a fast and convenient ‘paste-sampling’ method could be employed for trace contaminants on real samples, because EG-based Au NRs substrate is of flexibility and porosity. Thus, CV residue on shrimp could be determined lower than 1 ppb and thiram residue on grapes could be identified lower than 50 ppb. In addition to high sensitivity, the stability of EG-based Au NRs substrate is also very good. Even after acid/alkali pretreatment (pH = 4∼10) or 30 min of thermal treatment (T = 20∼100 °C), the enhancement of the substrate remained stable. What’s more, the substrate could be stored as long as 30 days. The highly stable, sensitive, cost-effective and easy-to-produce EG-based Au NRs substrates exhibit a great potential to promote application of SERS for routine analysis.
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Nanoscale
·
2022
·Vol. 14
·期 34
·12313-12321
·
IF 8.307
Yanying Cui,Linan Xu,Haitao Li,Xuan Wang,Fuwei Sun,Huan Wang,Xinguang Guo,Yihe Zhang,Hongbo Gao,Qi An
Surface-enhanced Raman scattering (SERS) is a rapid and promising detection technique for trace molecules. A central goal of research in this area is to achieve the highly sensitive detection of molecules built on a systematic understanding of enhancement mechanisms. Herein, we develop a Ag cluster@rGO composite nanostructure, which utilizes strong molecular adsorption to achieve ultrahigh SERS sensitivity. Ag clusters are prepared without additional reducing agents, leaving a low carbon footprint in the fabrication process. Finite-difference time-domain (FDTD) simulations show strong electromagnetic field enhancements generated at the edges and interstices of Ag clusters due to the specificity of their structure. Density Functional Theory (DFT) calculations show that the HOMO–LUMO energy gap value is significantly reduced when Ag cluster@rGO forms a composite system with the target molecule, which enables efficient charge transfer between the substrate and molecules, resulting in charge transfer enhancement. A detection limit of 10−14 M using our substrate can be achieved for the environmental pollutant dye rhodamine 6G (Rh6G). The detection limits of bisphenol A (BPA) and its derivatives reach nanomolar levels with good signal stability. More importantly, we demonstrate the ability to rapidly screen BPA migration in Chinese Baijiu. Our SERS platform can be further developed for environmental pollution control and food safety.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Jingyi Sun,Yanan Song,Mengyue Wang,Peng Zhao,Feng Gao,Junqi Li,Mingfeng Yang,Hui Yuan,Baoliang Sun,Ying Wang
Quantitative analysis of microRNAs (miRNAs) in a noninvasive manner is of vital importance for disease diagnosis and prognosis evaluation. However, conventional strategies for realizing accurate, simple, and sensitive detection of target molecules are still a challenge, especially for miRNAs due to their low abundance and susceptibility in the complex biological environment. Here, a novel surface-enhanced Raman scattering (SERS) strategy was established for quantitative detection and monitoring of miRNA-21-5p (miR-21-5p) in living cells and in vivo cerebrospinal fluid (CSF) by applying hairpin DNA (hpDNA)-conjugated gold nanostars (GNSs) SERS probes combined with acupuncture-based technology. This strategy enabled ultrasensitive exploration toward miR-21-5p in a wide range from 1 fM to 100 pM in cell lysates. Moreover, SERS analysis facilitated the detection and long-term monitoring for in vivo miR-21-5p noninvasively. This developed strategy promises to offer a powerful method for the analysis of multiple biomolecules in single cells and living bodies.
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POLYMER
·
2022
·
IF 4.432
Yongjiang Li,Xiaoyan Ma,Jingyu Ma,Zongwu Zhang,Zhaoqi Niu,Fang Chen
A new facile fabrication process of the polymer/Ag core-shell microspheres is demonstrated via the reverse breath figure method accompanied by in situ reduction. In contrast to traditional approaches, which require the prior preparation of polymer microspheres, the star-shaped polyhedral oligomeric silsesquioxane (POSS) amphiphilic block copolymers solution containing AgNO 3 is cast under ethanol vapor for the formation of polymer/AgNO 3 core-shell microspheres in one step. The influence of polymer molecular structures, solvent, non-solvent and metal salt on the morphology of polymer microspheres was analyzed. Then the AgNO 3 can be reduced in situ to form Ag nanoparticles on the surface of polymer microspheres, which resulted in the formation of polymer/Ag core-shell microspheres. The reduced Ag nanoparticles were homogeneously deposited on the surfaces of polymer microspheres, which can exhibit good performance in the surface-enhanced Raman scattering (SERS). The polymer/Ag core-shell microspheres substrate showed a high detection sensitivity (enhancement factor 2.49 × 10 6 ) with excellent uniformity and reproducibility for Rhodamine 6G (R6G) (relative standard deviation of the intensity at 612 cm −1 was 6.19%＜20%). The proposed strategy is facile to implement and thus a promising new method for the preparation of polymer/metal core-shell microspheres.
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Analytical Methods
·
2022
·Vol. 14
·期 11
·1134-1139
·
IF 3.532
Xiaojuan Zhao,Dan Sun,Man Yu,Yan Xu,Hui Xie
Montmorillonite (MMT) coated with roughened noble metal nanoparticles are novel hybrid nanocomposite with a wide range of applications including agriculture, materials science and biomedical engineering. Herein, we developed a hybrid nanocomposite (MMT/AgNPs) based on MMT coated with silver nanoparticles (AgNPs), which can be used as a cost-effective and efficient surface-enhanced Raman spectroscopy (SERS) substrate for the detection of pesticides in fruits and vegetables. MMT itself is negatively charged and can be assembled with positively charged AgNPs through electrostatic interactions. Moreover, MMT has a layered 2D structure that possesses a large surface area, which can load a large number of AgNPs to form more SERS hotspots for the ultrasensitive measurement. SERS performance of the MMT/AgNPs nanocomposite was tested by 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) and the substrate can obtain the strongest SERS enhancement effect with the volume ratio of MMT/AgNPs of 1 : 10. These substrates were applied in the measurement of thiram in apples and spinach samples by SERS. Detection limits of pesticide molecules of 5.0 × 10−8 M and 1.0 × 10−7 M in apples and spinach, respectively, were obtained. Most importantly, MMT nanosheets are a robust platform that allowed AgNPs to be evenly and thoroughly distributed and stabilized over the substrate, improving the repeatability and stability of SERS detection. These results reveal that the MMT/AgNPs nanocomposites are suitable substrates for the real-world SERS analysis of pesticide and other contaminants in complex food matrices.
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