JOURNAL OF MATERIALS SCIENCE
·
2021
·Vol. 56
·期 14
·8871-8886
·
IF 4.22
Pi Menghan,Jiang Lichao,Wang Zhisen,Cui Wei,Shi Lingying,Ran Rong
As a new two-dimensional material, MXene has caused extensive research in field of supercapacitor, electromagnetic shielding, strain sensing field due to its excellent mechanical properties and electrical conductivity. MXene with good water dispersibility can be used as an ideal nano-material to enhance the conductivity of hydrogels. However, MXene is easy to accumulate in the hydrogel system, and there are rare articles on MXene hydrogels. In this paper, we combine cellulose nanocrystals (CNCs) and MXene to prepare CNC/MXene nanosheets and take advantage of CNC’s excellent dispersibility in water and the electrostatic repulsion between CNCs to avoid the accumulating of MXene and enhance the mechanical properties of the hydrogel. Based on it, we prepared a physical–chemical double crosslinking and double-network CNC/MXene hydrogel by in situ polymerization and cycle freeze-thaw method. The CNC/MXene hydrogel we prepared exhibited excellent mechanical properties; it could be stretched to 7 times its original length and could reach 1.025 MPa tensile stress. When the compression strain was 80%, the compression stress could reach 1.1 MPa. After unloading the compression pressure, it could rapidly return to its original length within 0.17 s, which would impact greatly on the practical application of CNC/MXene hydrogel. What's more, the conductivity of it was up to 0.4 S/m and the thermal conductivity was 0.38 W/mK. So, the hydrogel prepared by us has a wide range of application prospects in the fields of human motion monitoring, electronic skin, man–machine interface, etc.
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CELLULOSE
·
2021
·Vol. 28
·期 3
·1715-1729
·
IF 5.044
Liu Hongyu,Yang Lin,Zhan Yifei,Lan Jianwu,Shang Jiaojiao,Zhou Mi,Lin Shaojian
In this work, a durable and sunlight-driven self-cleaning superhydrophobic cotton fabric was developed as a multifunctional oil/water separation material. First of all, Ag/AgCl was successfully deposited on the polydopamine modified cotton fabric surface by electrostatic adsorption, then the Ag/AgCl loaded fabric was coated by the polydimethylsiloxane (PDMS) via simple dip-coating method. The as-prepared cotton fabric (PDMS-Ag/AgCl@CF) presented the superhydrophobic property with water contact angle about 155°. Meanwhile, the prepared PDMS-Ag/AgCl@CF exhibited great tolerance and resistance to broad pH and various organic solvents, and it can also withstand 50 cycles of abrasion or 60 min of ultrasound treatment without an apparent decrease of superhydrophobicity, indicating its outstanding mechanical durability. As expected, this durable superhydrophobic fabric can availably separate various oil/water mixtures by gravity-driven force with high separation efficiency (over 97.8%), and it possessed good reusability. Additionally, the PDMS-Ag/AgCl@CF exhibited an outstanding self-cleaning performance under sunlight irradiation, due to visible light photocatalytic performance of Ag/AgCl. Finally, the resultant cotton fabric displayed good antibacterial activity. Therefore, in consideration of its prominent comprehensive performances, it is expected that the PDMS-Ag/AgCl@CF can be used as a promising material for oily water remediation.
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3 Biotech
·
2020
·Vol. 10
·期 5
·1-13
·
IF 1.798
Liu Zhengying,Bao Jianying,Yang Huilin,Zhang Zhibin,Yan Riming,Zhu Du
Perylenequinones (PQ), a class of naturally occurring polypeptides, are widely used as a clinical drug for treating skin diseases and as a photodynamic therapy against cancers and viruses. In this study, the effects of different carbon sources on PQ biosynthesis by Shiraia sp. Slf14 were compared, and the underlying molecular mechanism of fructose as the sole carbon to enhance PQ production was investigated by transcriptome analysis. The results indicated that fructose enhanced PQ yield to 1753.64 mg/L, which was 1.73-fold higher than that obtained with glucose. Comparative transcriptome analysis demonstrated that most of the upregulated genes were related to transport systems, energy and central carbon metabolism in Shiraia sp. Slf14 cultured in fructose. The genes involved in glycolysis and pentose phosphate pathways, and encoding citrate synthase, ATP-citrate lyase, and acetyl-CoA carboxylase were substantially upregulated, resulting in increased overall acetyl-CoA and malonyl-CoA production. However, genes involved in gluconeogenesis, glyoxylate cycle pathway, and fatty acid synthesis were significantly downregulated, resulting in higher acetyl-CoA influx for PQ formation. In particular, the putative PQ biosynthetic cluster was upregulated in Shiraia sp. Slf14 cultured in fructose, leading to a significant increase in PQ production. The results of real-time qRT-PCR and related enzyme activities were also consistent with those of transcriptome analysis. These findings provide a remarkable insight into the underlying mechanism of PQ biosynthesis and pave the way for improvements in PQ production by Shiraia sp. Slf14.
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PubMed
高影响因子
Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-10
·
IF 6.352
Wen Yongdi,Liao Baosheng,Yan Xiaoxiao,Wu Zhenqiang,Tian Xiaofei
Background Hypocrellin A (HA) is a perylene quinone pigment with high medicinal value that is produced by Shiraia bambusicola Henn. (S. bambusicola) and Hypocrella bambusae (Berk. & Broome) Sacc. (Ascomycetes) with great potential in clinical photodynamic therapy. Submerged cultivation of S. bambusicola is a popular technique for HA production. However, there is not much research on how temperature changes lead to differential yields of HA production.Results The temperature regulation of submerged fermentation is an efficient approach to promote HA productivity. After a 32 °C fermentation, the HA content in the mycelia S. bambusicola (GDMCC 60438) was increased by more than three- and fivefold when compared to that at 28 °C and 26 °C, respectively. RNA sequencing (RNA-seq) analysis showed that the regulation of the expression of transcription factors and genes essential for HA biosynthesis could be induced by high temperature. Among the 496 differentially expressed genes (DEGs) explicitly expressed at 32 °C, the hub genes MH01c06g0046321 and MH01c11g0073001 in the coexpression network may affect HA biosynthesis and cytoarchitecture, respectively. Moreover, five genes, i.e., MH01c01g0006641, MH01c03g0017691, MH01c04g0029531, MH01c04g0030701 and MH01c22g0111101, potentially related to HA synthesis also exhibited significantly higher expression levels. Morphological observation showed that the autolysis inside the mycelial pellets tightly composted intertwined mycelia without apparent holes.Conclusion sThe obtained results provide an effective strategy in the submerged fermentation of S. bambusicola for improved HA production and reveal an alternative regulatory network responsive to the biosynthesis metabolism of HA in response to environmental signals.
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高影响因子
Drug Delivery and Translational Research
·
2020
·Vol. 11
·期 1
·169-181
·
IF 2.664
Wu Jun-Yong,Cai Jia-Xin,Li Yong-Jiang,Hu Xiong-Bin,Liu Xin-Yi,Wang Jie-Min,Tang Tian-Tian,Xiang Da-Xiong
For therapy of skin cancer, transdermal administration has been a potential way to enhance chemotherapy. However, the drug delivery efficacy remained unsatisfactory because of the physiological barriers from the skin to the tumor, which hindered the effect of 3,5,4′-trimethoxy-trans-stilbene (BTM), a drug that has toxicity to cancer. Herein, we prepared an oil-in-water (O/W) microemulsion to load BTM (BTM-ME) for transdermal therapy of melanoma. BTM-ME was characterized by size, zeta potential, and polymer disperse index (PDI). B16F10 melanoma cell line was used for cell experiments and animal models. And cell uptake, viability assay, and flow cytometry were to test the cell internalization and the ability of BTM-ME to induce cancer cell apoptosis. Skin penetration testing was to detect its penetration efficiency to the skin. And tumor-bearing mice were used to prove the improvement of anti-cancer efficacy of BTM-ME with the combination of Taxol. BTM was successfully loaded in O/W microemulsion, with a drug loading capacity of 24.82 mg/mL. BTM-ME can penetrate the skin and increase the retention of BTM in the epidermis. And the combination of Taxol and BTM-ME effectively suppressed tumor growth and has lower toxicity to normal organs. BTM-ME provides adjuvant therapy to cutaneous melanoma and the combination of Taxol and BTM-ME has the clinical potential for skin cancer therapy.
Graphical abstract
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高影响因子
TRANSITION METAL CHEMISTRY
·
2020
·Vol. 45
·期 4
·253-266
·
IF 1.366
An Si-Ying,Su Yuan-Yuan,Qi Xin,Zhang Rong-Lan,Ma Yan-Ling,Zhao Jian-She
Abstract A series of Zn(II)-based complexes as photoinduced carbon-monoxide-releasing molecules (photoCORMs), viz. ( L )Zn( R )]ClO 4 with L = tris(pyridine-2-ylmethyl)amine (TPA), N , N -((5-estermethyl-2-pyridyl)methyl)-bis(2-pyridylmethyl) amine (5-COOCH 3 TPA), N , N -(bis(5-estermethyl-2-pyridyl)methyl)-(2-pyridylmethyl) amine [(5-COOCH 3 ) 2 TPA], or N , N -((6-estermethyl-2-pyridyl)methyl)-bis(2-pyridylmethyl) amine (6-COOCH 3 TPA) and R = 4-dimethylaminoflavonol [4-N(Me) 2 FLH], 4-diethylaminoflavonol [4-N(Et) 2 FLH], or 4-methoxyflavonol (4-MeOFLH), have been prepared and characterized. When dissolved in CH 3 CN or 1:1 H 2 O:dimethylsulfoxide (DMSO) in presence of O 2 , visible light ( λ max ranging from 417 to 462 nm) induced dioxygenase-type reactions to release CO quantitatively from these complexes. Moreover, the intracellular uptake and CO releasing reactivity were confirmed by the change of their fluorescent properties observed under fluorescence microscopy. The cytotoxicity of the complexes demonstrates that they exhibit good biocompatibility in the concentration range from 1 to 50 μM. The solid complexes are stable in air for more than 60 days, and their aqueous solutions are also stable for 96 h under anaerobic or dark condition. The remarkable stability and biocompatibility of the complexes are indispensable properties for use in pharmaceutical chemistry. Graphic abstract
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高影响因子
AAPS PHARMSCITECH
·
2021
·Vol. 22
·期 1
·1-10
·
IF 3.246
Zhao Yunchun,Zheng Haili,Wang Xiaorong,Zheng Xiaoling,Zheng Yongquan,Chen Yue,Fei Weidong,Zhu Jiahuan,Wang Wenxi,Zheng Caihong
Novel cationic lipid-based liposomes prepared using an amphiphilic cationic lipid material, N , N -dimethyl-( N ′, N ′-di-stearoyl-1-ethyl)1,3-diaminopropane (DMSP), have been proposed to enhance the transfection of nucleic acids. Herein, we designed and investigated liposomes prepared using DMSP, soybean phosphatidylcholine, and cholesterol. This novel gene vector has high gene loading capabilities and excellent protection against nuclease degradation. An in vitro study showed that the liposomes had lower toxicity and superior cellular uptake and transfection efficiency compared with Lipofectamine 2000. An endosomal escape study revealed that the liposomes demonstrated high endosomal escape and released their genetic payload in the cytoplasm efficiently. Mechanistic studies indicated that the liposome/nucleic acid complexes entered cells through energy-dependent endocytosis that was mediated by fossa proteins. These results suggest that such cationic lipid-based liposome vectors have potential for clinical gene delivery.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2022
·Vol. 33
·期 10
·1-13
·
IF 4.727
Su Zhixiang,Xing Yuehao,Wang Fei,Xu Zeqin,Gu Yongquan
There are no suitable methods to develop the small-calibre tissue-engineered blood vessels (TEBVs) that can be widely used in the clinic. In this study, we developed a new method that combines electrospinning and in-body tissue architecture(iBTA) to develop small-calibre TEBVs. Electrospinning imparted mechanical properties to the TEBVs, and the iBTA imparted biological properties to the TEBVs. The hybrid fibres of PLCL (poly(L-lactic-co-ε-caprolactone) and PU (Polyurethane) were obtained by electrospinning, and the fibre scaffolds were then implanted subcutaneously in the abdominal area of the rabbit (as an in vivo bioreactor). The biotubes were harvested after four weeks. The mechanical properties of the biotubes were most similar to those of the native rabbit aorta. Biotubes and the PLCL/PU vascular scaffolds were implanted into the rabbit carotid artery. The biotube exhibited a better patency rate and certain remodelling ability in the rabbit model, which indicated the potential use of this hybridization method to develop small-calibre TEBVs.
Sketch map of developing the biotube. The vascular scaffolds were prepared by electrospinning (A). Silicone tube was used as the core, and the vascular scaffold was used as the shell (B). The vascular scaffold and silicone tube were implanted subcutaneously in the abdominal area of the rabbit (C). The biotube was extruded from the silicone tube after 4 weeks ofembedding (D). The biotube was implanted for the rabbit carotid artery (E).
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 3
·1-11
·
IF 6.408
Wang Ying,Wang Tian,Wang Minglu,Wang Jun,Xu Zhixiang,Zhang Hongyan
The simple and rapid commercial colloidal gold test strip can only be used for qualitative or semi-quantitative detection, accompanied by weak detectability and false negative experimental results. Herein, a photothermal test strip assay which combined test strip with a portable photothermal card reader was established to achieve quantitative detection with excellent detectability. According to the photothermal effect produced by gold nanoparticles (GNPs) captured on the test line, the signal could be recorded by the reader. Thirteen food hazards including veterinary drug residues and pesticide residues were tested; the photothermal detectability in actual samples were about 23 (methyl parathion), 7 (enrofloxacin), 6 (sarafloxacin), 8 (sulfadiazine), 12 (sulfamethazine), 7 (paraquat), 6 (malachite green), 11 (amantadine), 13 (nitrofurazone), 6 (diethylstilbestrol), 12 (estriol), 21 (estrone), and 26 (17β-estradiol) times better than the visual detectability. Our results demonstrated that the photothermal test strip assay could be used for sensitive, rapid, and quantitative detection of residues of food hazards. Graphical abstract
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高影响因子
Nature Communications
·
2026
·
IF 18.1
Gao Lu,Yuan Zhuolei,Huang Tongling,Li Pengda,Zhong Yanting,Gao Jun,Luo Yifan,Li Bin,Pan Haobo,Ye Keqiang,Guan Min
Metabolic dysregulation under nutrient stress disrupts bone homeostasis. Although acetate metabolism is linked to diabetes and alcoholism, its role in the skeletal complications of these conditions remains unknown. Here we identify acetyl-CoA synthetase 1 (ACSS1) as a key stress responder that mediates mitonuclear communication in bone marrow skeletal stem cells. ACSS1-driven rewiring of acetate metabolism impairs cellular metabolic flexibility, mitochondrial function, and osteogenesis. Mechanistically, nutrient excess from glucose, alcohol or acetate upregulates ACSS1 via a mitochondrial ROS activated integrated stress response-CEBPB pathway. Maladaptive ACSS1 accumulation enforces excessive acetyl-CoA production from either exogenous or glucose/ethanol-derived endogenous acetate, promoting mitochondrial protein hyperacetylation specifically inactivating ME2 via lysine 94 acetylation. This ACSS1-triggered ME2 inactivation disturbs the malate-aspartate shuttle, leading to a self-perpetuating cycle of redox imbalance and oxidative stress that exacerbates mitochondrial dysfunction and osteogenic impairment. Importantly, genetic deletion of Acss1 or AAV-delivered non-acetylatable ME2 mutant in osteoprogenitor cells restores mitochondrial resilience, osteogenic capacity, and bone formation, protecting against osteoporosis or osteonecrosis in male mouse models of type 2 diabetes and chronic alcohol consumption. These results unveil an essential role for ACSS1 in controlling metabolic flexibility and osteogenesis, identifying ACSS1 as a potential therapeutic target for metabolic stress-related bone disorders.
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Molecular Horticulture
·
2026
·Vol. 6
·期 1
·75
·
IF 10
Chen Jingwen,Li Airong,Wu Tianqi,Huang Zhenyu,Li Xiyao,Xu Shan,Wu Yujie,Tian Yuan,Chen Weiwei,Quan Xinhua,Yao Xiangtan,Lin Sue,Feng Xuping,Huang Li
The morphological diversification of leaf margins reflects the evolutionary reshaping of conserved developmental programs. However, fully deciphering the underlying molecular basis remains challenging due to difficulties in phenotype quantification and the incomplete characterization of complex regulatory networks. In this study, we established a computer-vision framework to decode leaf lobe trait and identified the HD-ZIP III factor PHABULOSA ( BcPHB ), combining genome-wide association studies (GWAS) and selective sweep analysis in Brassica campestris . We found that promoter variation defines two major haplotypes, BcPHB HapI and BcPHB HapII , driving divergent expression levels associated with entire and lobed leaves. Functionally, BcPHB knockdown lines ( BcPHB ami ) exhibited lobed margins, whereas complementation of lobed accessions with the BcPHB HapI promoter partially restored the entire-leaf phenotype. Similarly, overexpression of LATE MERISTEM IDENTITY 1 ( BcLMI1 ), a conserved regulator of leaf lobation, phenocopied the BcPHB ami lines. Mechanistically, BcPHB and BcLMI1 form a reciprocal negative feedback loop that stabilizes margin patterning. Furthermore, domestication-driven selection of BcPHB HapI facilitated the transition from ancestral lobed leaves to cultivated entire forms. This study reveals a pivotal developmental module and illustrates how regulatory evolution drives morphological diversification in crops.
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高影响因子
BMC PLANT BIOLOGY
·
2022
·Vol. 22
·期 1
·1-12
·
IF 5.26
Yue Runqing,Sun Qi,Ding Jianguo,Li Wenlan,Li Wencai,Zhao Meng,Lu Shouping,Zeng Tingru,Zhang Hua,Zhao Suxian,Tie Shuanggui,Meng Zhaodong
Background Maize rough dwarf disease (MRDD), caused by rice black-streaked dwarf virus (RBSDV) belonging to the Fijivirus genus, seriously threatens maize production worldwide. Three susceptible varieties (Ye478, Zheng 58, and Zhengdan 958) and two resistant varieties (P138 and Chang7–2) were used in our study. Results A set of ATP-binding cassette subfamily B (ABCB) transporter genes were screened to evaluate their possible involvements in RBSDV resistance. In the present study, ZmABCB15 , an ABCB transporter family member, was cloned and functionally identified. Expression analysis showed that ZmABCB15 was significantly induced in the resistant varieties, not in the susceptible varieties, suggesting its involvement in resistance to the RBSDV infection. ZmABCB15 gene encodes a putative polar auxin transporter containing two trans-membrane domains and two P-loop nucleotide-binding domains. Transient expression analysis indicated that ZmABCB15 is a cell membrance localized protein. Over-expression of ZmABCB15 enhanced the resistance by repressing the RBSDV replication ratio. ZmABCB15 might participate in the RBSDV resistance by affecting the homeostasis of active and inactive auxins in RBSDV infected seedlings. Conclusions Polar auxin transport might participate in the RBSDV resistance by affecting the distribution of endogenous auxin among tissues. Our data showed the involvement of polar auxin transport in RBSDV resistance and provided novel mechanism underlying the auxin-mediated disease control technology.
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高影响因子
危险品化学品经营许可证（带存储）