INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2023
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IF 5.8
Fangqian Sui,Zengjun Fang,Lingjun Li,Xinhuan Wan,Yongqing Zhang,Xiaoqing Cai
Multifunctional nanoparticles have attracted significant attentions for oncology and cancer treatment. In fact, they could address critical point for tumour treatment by creating a stimuli-responsive targeted drug delivery system that can exist stably in the systemic circulation, efficiently penetrate the tumour tissue, and then accumulate in tumour cells in large quantities. A novel stepwise pH-responsive multifunctional nanoparticles (FPDPCNPs/DTX) for targeted delivery of the antitumour drug docetaxel (DTX) is prepared by coating a tumour acidity-sensitive “sheddable” FA modified β-carboxylic amide functionalized PEG layer (folic acid-polyethylene glycol-2,3-dimethylmaleic anhydride, FA-PEG-DA) on the cationic drug-loaded core (poly(β-amino ester-cholesterol, PAE-Chol) through electrostatic interaction in this study. The charge shielding behaviour of the FPDPCNPs/DTX was confirmed by zeta potential assay. The surface charges of the nanoparticles can change from positive to negative after PEG coating. The IC 50 values of FPDPCNPs/DTX was 3.04 times higher than that of PEG “unsheddable” nanoparticles in cytotoxicity experiments. The results of in vivo experiment further showed that FPDPCNPs/DTX had enhanced tumour targeting effect, the tumour inhibition rate of FPDPCNPs/DTX was as high as 81.99%, which was 1.51 times that of free DTX. Under a micro acidic environment and folate receptor (FR)-mediated targeting, FPDPCNPs/DTX contributed to more uptake of DTX by MCF-7 cells. In summary, FPDPCNPs/DTX as a multifunctional nano-drug delivery system provides a promising strategy for efficiently delivering antitumour drugs.
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Haifeng Lu,Qi Lu,Hongwu Sun,Zhongkun Wang,Xiang Shi,Yuling Ding,Xiang Ran,Jing Pei,Yubo Pan,Qunlin Zhang
Intracellular reactive oxygen species (ROS) are closely associated with cancer cell types. Therefore, ROS-based pattern recognition is a promising strategy for precise diagnosis of cancer, but such a possibility has never been reported yet. Herein, we proposed an ROS-responsive fluorescent sensor array based on pH-controlled histidine-templated gold nanoclusters (AuNCs@His) to distinguish cancer cell types and their proliferation states. In this strategy, three types of AuNCs@His with diverse fluorescence profiles were first synthesized by only adjusting the pH value. Upon the addition of various ROS, fluorescence quenching of three types of AuNCs@His occurred with different degrees, thereby forming unique optical “fingerprints”, which were well-clustered into several separated groups without overlap by principal component analysis (PCA). The sensing mechanism was attributable to the oxidation of AuNCs@His by ROS, as revealed by X-ray photoemission spectroscopy, Fourier transform infrared spectroscopy, 1H nuclear magnetic resonance spectroscopy, and electrospray ionization mass spectrometry. Based on the ROS-responsive sensing pattern, cancer cell types were successfully differentiated via PCA with 100% accuracy. Additionally, the proposed sensor array exhibited excellent performance in distinguishing the proliferation states of cancer cells, which was supported by the results of the Ki-67 immunohistochemistry assay. Overall, the ROS-responsive fluorescent sensor array can serve as a promising tool for precise diagnosis of cancer, indicating great potential for clinical application.
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EUROPEAN POLYMER JOURNAL
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2023
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IF 6
Shulan Jiang,Li Xia,Hao Ma,Tingting Yang,Linmao Qian
Smart hydrogels have received attentions for the great potentials in biomedical and robots applications. However, the deformation performance of hydrogels still needs to be improved, especially for the pH and thermal dual responsive hydrogels. In this study, a pH and temperature dual-responsive poly(acrylamide-acrylic acid-3-acrylamidophenylboronic acid)/Poly(N-isopropylacrylamide) (P(AAm-AAc-3-AAPBA)/PNIPAM) bilayer structures have been prepared by UV polymerization. The PNIPAM layer shrinks/swells when the temperature is higher/lower than the low critical solution temperature , while P(AAm-AAc-3-AAPBA) layer will swell/shrink when pH is too high/low. The volume change trend of the two layer is different, resulting in the bending behaviors of P(AAm-AAc-3-AAPBA)/PNIPAM hydrogel actuator. The bidirectional bending performance can be reversibly, repeatedly, and precisely controlled by pH and temperature-induced cooperative swelling-shrinking properties from both layers. The bending angle of the bilayer structure achieves about 1420° at the environment of pH 2 and 55 ℃. It takes about 70 s to convert the bending angle of the bilayer structures from 355° (10 ℃, pH 2) to −360° (55 ℃, pH 12). In addition, three kinds of biomimetic hydrogel structures, such as mechanical gripper, bionic flower and bionic hand, have been prepared and demonstrated good performance. The obtained P(AAm-AAc-3-AAPBA)/PNIPAM hydrogels exhibit excellent pH and thermal responsive bending properties, which enable them as an ideal smart hydrogel system.
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CARBOHYDRATE POLYMERS
·
2023
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IF 11.2
Shuifang Mao,Yanming Ren,Shiguo Chen,Donghong Liu,Jinhu Tian,Xingqian Ye
Favorable hydrogels can be used as a material to deliver bioactive molecules and improve the stability of bioactive substances, while their safety needs to be improved. In this study, protocatechuic acid (PCA) and Fe 3+ were rapidly self-assembled to form a metal-phenolic network under different pH conditions, and then sodium alginate (SA) was added to prepare the SA/PCA/Fe hydrogel without adding other chemical reagents. The structural characteristic of SA/PCA/Fe hydrogel was characterized by infrared spectroscopy, X-ray diffraction analysis and scanning electron microscopy. The results showed that the structures of SA/PCA/Fe hydrogels prepared at different pH values were significantly different. The texture analysis, water-holding measurement and rheological analysis indicated that the SA/PCA/Fe hydrogel showed higher gel strength, water holding capacity and storage modulus. Thermogravimetric analysis illuminated that the SA/PCA/Fe hydrogel enhanced the thermal stability of free anthocyanins through encapsulating anthocyanins. Moreover, in vitro simulated digestion experiment revealed that SA/PCA/Fe hydrogel could control the release of anthocyanins in the simulated gastrointestinal tract. To sum up, this present study might provide a safer and feasible way for the delivery of bioactive substances.
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Materials Today Bio
·
2023
·
IF 8.2
Zhong-Hua Chen,De-Yan Du,Yi-Fei Fu,Jun-Jie Wu,Dan-Yang Guo,Yue-Yue Li,Meng-Nan Chen,Zheng-Dong Yuan,Kai-Wen Zhang,Zhen-Yu Zhang,Xia Li,Feng-Lai Yuan
Bone targeted delivery of estrogen offers great promise for the clinical application of estrogen in the treatment of postmenopausal osteoporosis (PMOP). However, the current bone-targeted drug delivery system still has several issues that need to be solved, such as the side effects of bone-targeted modifier molecules and the failure of the delivery system to release rapidly in the bone tissue. It is important to aggressively search for new bone-targeted modifier molecules and bone microenvironment-responsive delivery vehicles. Inspired by the distribution of citric acid (CA) mainly in bone tissue and the acidic bone resorption microenvironment, we constructed a CA-modified diblock copolymer poly(2-ethyl-2-oxazoline)–poly(ε-caprolactone) (CA-PEOz) drug delivery system. In our study, we found that the CA modification significantly increased the bone targeting of this drug delivery system, and the delivery system was able to achieve rapid drug release under bone acidic conditions. The delivery system significantly reduced bone loss in postmenopausal osteoporotic mice with a significant reduction in estrogenic side effects on the uterus. In summary, our study shows that CA can act as an effective bone targeting modifier molecule and provides a new option for bone targeting modifications. Our study also provides a new approach for bone-targeted delivery of estrogen for the treatment of PMOP.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2023
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IF 4.4
Simin Lin,Chunmei Lai,Zejie Huang,Wei Liu,Lei Xiong,Yuxin Wu,Yanqiao Jin
Synthesis of lignin-based carbon dots (LCDs) with high quantum yield (QY), stable fluorescence properties and biocompatibility has been a challenge. Here, we propose an improved two-step strategy for producing high-quality LCDs from enzymatic hydrolysis lignin (EHL). The p-aminobenzenesulfonic acid used in the strategy not only provides nitrogen and sulfur elements, but also tailors the disordered three-dimensional structure of EHL. The successful co-doping of N and S elements favors the reduction of the optical energy bandgap (E g ), resulting in a high QY of 45.05% for LCDs. The LCDs exhibited superior selectivity and sensitivity for Fe 3+ with a limit of detection (LOD) of 0.15 μM when Fe 3+ concentration was 50–500 μM. In addition, LCDs demonstrated significant fluorescence in HepG2 cells and HepG2 cells loaded with LCDs at a concentration of 80 μg/mL showed good viability, suggesting that they are suitable for in vivo applications. The luminescent centers of LCDs change during pH regulation and thus show a special visual response to pH changes, making them have great potential for detecting metabolism in living cells. This work provides a novel and low-cost method for fabricating sustainable fluorescent probes for chemical sensing and bioimaging.
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Biomaterials Science
·
2023
·
IF 6.6
Siyi Li,Qinglin Wang,Yingying Ren,Pengfei Zhong,Pengtao Bao,Shanyue Guan,Xiaochen Qiu,Xiaozhong Qu
The development of cancer treatment is of great importance especially in the early stage. In this work, we synthesized pH-sensitive amphiphilic ruthenium complex containing two alkyl chains and two PEG chains which was utilized as oxygen sensitive fluorescent probe for co-assembly with lipids to harvest a liposomal delivery system (RuPC) for the encapsulation of a photothermal agent indocyanine green (ICG). The resultant ICG encapsulated liposome (RuPC@ICG) enabled to deliver ICG into cells via membrane fusion pathway by which the ruthenium complex was localized in the cell membrane for better detecting the extracellular oxygen concentration. Such characteristic endowed the ratiometric imaging feature to distinguish tumour location from normal tissues at just 3 days after cancer cells were implanted by monitoring the hypoxia condition and tracing the metabolism. Moreover, the pH sensitivity of the liposomes favoured the cell uptake, and improved the anti-tumour efficiency of the formulation in vivo under NIR irradiation. Should liposomal systems have less safety issue, our work not only provided a facile way for the construction of theragnostics by combining phototherapy with photoluminescent imaging, but hopefully paving the way for the clinical translation from bench to bedside.
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FOOD HYDROCOLLOIDS
·
2023
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IF 10.7
Hanyu Li,Minghui Zhao,Sijie Zhou,Huajiang Zhang,Jing Wang,Ning Xia,Yujia Liu,Shihui Hua,Guixin Tan
Curcumin is a hydrophobic polyphenol with various health benefits, but its poor water solubility and stability limit its application in the food and pharmaceutical industries. In this study, a pH-driven method was developed to prepare ovalbumin (OVA)-curcumin nanocomplexes without organic solvents, reducing the environmental impact. The results showed that curcumin was encapsulated in OVA with a high efficiency of over 95% and remained in an amorphous state within the protein molecules. Molecular docking and spectroscopic analyses revealed that curcumin was mainly bound and stabilized by hydrogen bonds and hydrophobic interactions in the hydrophobic core of OVA. The binding strength of the intermediate stage of the pH-driven method was stronger than that of the final stage, due to the exposure of OVA molecular binding sites and the involvement of electrostatic attraction. Furthermore, the potential of polysaccharide blending to enhance the stability of the nanocomplexes was investigated. The results showed that sodium alginate effectively enhanced the photostability and thermal stability of the OVA-curcumin nanocomplexes by co-solubilization and electrostatic complex formation. In addition, simulated in vitro digestion also demonstrated that the pH-driven method combined with polysaccharide blending increased the bioaccessibility of curcumin in nanocomplexes from 17.79% to 52.54%. Therefore, the pH-driven method coupled with polysaccharide blending ensures the encapsulation and stabilization of hydrophobic bioactive substances for use in their delivery systems to improve bioaccessibility.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 4
·647-653
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IF 3.1
Li Miao,Hao Yashi,Yang Zuobo,Yun Jimmy,Liang Xin
Ruthenium(Ru) and its derivatives have been widely studied as oxygen evolution reaction(OER) electrocatalysts in acidic water electrolysis due to their inherent electronic properties and high oxygen evolution activity. A facile pH-induced size regulation approach for Ru nanocrystals has been developed by introducing NaOH and CH 3 COOH in a polyol system. The size of Ru particles decreases with the increase of the dosage of NaOH and increases at a certain dosage of CH 3 COOH. The formation mechanism of ruthenium nanocrystals was investigated through a series of characterizations and kinetic experiments. The electrocatalytic activities of the electrocatalysts derived from these Ru particles were studied toward OER to investigate the influence of particle size on their electrocatalytic properties. Moreover, the prepared electrocatalysts were applied as anodic materials in the proton exchange membrane(PEM) electrolysis cell and demonstrated excellent performance.
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ACS Nano
·
2023
·
IF 17.1
Chaojie Ren,Danni Zhong,Yuchen Qi,Chaoyi Liu,Xingyu Liu,Shihong Chen,Sheng Yan,Min Zhou
The oral form of insulin is more convenient and has better patient compliance than subcutaneous or intravenous insulin. Current oral insulin preparations, however, cannot overcome the enzyme barrier, chemical barrier, and epithelial barrier of the gastrointestinal tract completely. In this study, a microalgae-based oral insulin delivery strategy (CV@INS@ALG) was developed using Chlorella vulgaris (CV)-based insulin delivery system cross-linking with sodium alginate (ALG). CV@INS@ALG could overcome the gastrointestinal barrier, protect insulin from harsh gastric conditions, and achieve a pH-responsive drug release in the intestine. CV@INS@ALG might contribute to two mechanisms of insulin absorption, including direct insulin release from the delivery system and endocytosis by M cells and macrophages. In the streptozotocin (STZ)-induced type 1 diabetic mouse model, CV@INS@ALG showed a more effective and long-lasting hypoglycemic effect than direct insulin injection and did not cause any damage to the intestinal tract. Additionally, the long-term oral administration of the carrier CV@ALG effectively ameliorated gut microbiota disorder, and significantly increased the abundance of probiotic Akkermansia in db/db type 2 diabetic mice, thereby enhancing the insulin sensitivity of mice. Microalgal insulin delivery systems could be degraded and metabolized in the intestinal tract after oral administration, showing good biodegradability and biosafety. This insulin delivery strategy based on microalgal biomaterials provides a natural, efficient, and multifunctional solution for oral insulin delivery.
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Frontiers in Chemistry
·
2023
·
IF 5.5
Jiapeng Deng,Su Liu,Guoqing Li,Yien Zheng,Weifei Zhang,Jianjing Lin,Fei Yu,Jian Weng,Peng Liu,Hui Zeng
Introduction: Chemotherapy is a common strategy for the treatment of osteosarcoma. However, its therapeutic efficacy is not ideal due to the low targeting, lowbioavailability, and high toxicity of chemotherapy drugs. Nanoparticles can improve the residence time of drugs at tumor sites through targeted delivery. This new technology can reduce the risk to patients and improve survival rates. To achieve this goal, we developed a pHsensitive charge-conversion polymeric micelle [mPEG- b -P(C7- co -CA) micelles] for osteosarcoma-targeted delivery of cinnamaldehyde (CA). Methods: First, an amphiphilic cinnamaldehyde polymeric prodrug [mPEG- b -P(C7- co -CA)] was synthesized through Reversible Addition-Fragmentation Chain Transfer Polymerization (RAFT) polymerization and post-modification, and self-assembled into mPEG- b -P(C7- co -CA) micelles in an aqueous solution. The physical properties of mPEG- b -P(C7- co -CA) micelles, such as critical micelle concentration (CMC), size, appearance, and Zeta potential were characterized. The CA release curve of mPEG- b -P(C7- co -CA) micelles at pH 7.4, 6.5 and 4.0 was studied by dialysis method, then the targeting ability of mPEG- b -P(C7- co -CA) micelles to osteosarcoma 143B cells in acidic environment (pH 6.5) was explored by cellular uptakeassay. The antitumor effect of mPEG- b -P(C7- co -CA) micelles on 143B cells in vitro was studied by MTT method, and the level of reactive oxygen species (ROS) in 143B cells after mPEG- b -P(C7- co -CA) micelles treatment was detected. Finally, the effects of mPEG- b -P(C7- co -CA) micelles on the apoptosis of 143B cells were detected by flow cytometry and TUNEL assay. Results: An amphiphilic cinnamaldehyde polymeric prodrug [mPEG- b -P(C7- co -CA)] was successfully synthesized and self-assembled into spheric micelles with a diameter of 227 nm. The CMC value of mPEG- b -P(C7- co -CA) micelles was 25.2 mg/L, and it showed a pH dependent release behavior of CA. mPEG- b -P(C7- co -CA) micelles can achieve chargeconversion from a neutral to a positive charge with decreasing pHs. This charge-conversion property allows mPEG- b -P(C7- co -CA) micelles to achieve 143B cell targeting at pH 6.5. In addition, mPEG- b -P(C7- co -CA) micelles present high antitumor efficacy and intracellular ROS generation at pH 6.5 which can induce 143B cell apoptosis. Discussion: mPEG- b -P(C7- co -CA) micelles can achieve osteosarcoma targeting effectively and enhance the anti-osteosarcoma effect of cinnamaldehyde in vitro . This research provides a promising drug delivery system for clinical application and tumor treatment.
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Foods
·
2023
·Vol. 12
·期 9
·1857
·
IF 5.2
Ruoting Liu,Wenrui Chi,Qihao Zhu,Hailan Jin,Jian Li,Lijuan Wang
A cellulose-based matrix for monitoring milk freshness (MF) was produced from rice straw particles (RSPs) in a 0.125–0.150 mm that was bis-quaternized to attach bromocresol purple (BP) as a sensor. Under alkali conditions, the obstinate structure of the rice straw had opened, thereby improving the accessibility of the cellulose. Bis-quaternization created more adsorption sites for BP. The maximum adsorption capacity was 97.68 mg/g. The sensors were interwoven with cellulosic fibers to form the cellulose-based label with a relatively loose three-dimensional structure via hydrogen bonds. As the proportion of BP-BCRPs was increased from 10% to 40%, the air permeability of the label increased from 3.76 to 15.01 mm/s, which increased the response to the tested gases (10.12 s for 1 mL of acetic acid). The intelligent label exhibited excellent sensitivity at pH values of 3–9 with highly saturated color changes. During the storage period, the label color shifted from blue-purple to yellow as acidity was increased from 17.24 to 19.8 °T due to capillarity action, providing a timely warning to consumers. The prepared colorimetric porous intelligent cellulose-based label is suitable for monitoring of MF.Keywords:bis-quaternary ammonium salt;porous cellulose-based label;capillarity;pH sensitivity;milk freshness
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危险品化学品经营许可证（带存储）