MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yuanzhen He,Dengjie Zhong,Yunlan Xu,Ran Jiang,Pengfei Liao,Jiayou Zhang
In this work, Ti/SnO 2 -Sb 2 O 4 -La/β-PbO 2 electrode was successfully prepared by electrodeposition method. The surface morphology, crystal structure, elemental states and electrochemical characteristics of the electrode were examined by SEM, XRD, XPS, CV, and LSV. The results demonstrated that the conductivity, specific surface area, electrochemical oxidation performance and stability of the β-PbO 2 coated electrode with SnO 2 -Sb 2 O 4 -La as the intermediate layer were greatly improved. Under the conditions of initial rhodamine B (RhB) concentration of 20 mg/L, initial pH of 3.0, electrolyte concentration of 0.1 M, and current density of 30 mA cm −2 , the removal rates of color and TOC reached 100 % and 83.64 % after 20 mins and 30 mins, respectively. The electrode can also completely degrade methylene blue and methyl orange wastewater after 30 mins under the same conditions. The accelerated lifetime of the electrode reached 175 mins (1000 mA cm −2 , 1 M H 2 SO 4 ), and the actual lifetime was up to 3208 hrs. Based on the results of free radical quenching experiment and GC–MS detection, RhB degradation pathway was proposed: the aniline-based conjugated system of RhB molecule was attacked by the combined action of free radicals (indirect oxidation) and electrocatalysis (direct oxidation), which decomposed macromolecules into small molecules and ultimately mineralized them into CO 2 and H 2 O. This work is expected to provide a fresh research concept for preparing DSA electrodes with excellent catalytic performance and high stability.
✓ 已验证
产品关联
DOI
高影响因子
ACS Chemical Neuroscience
·
2025
·
IF 3.9
Ying Sun,Yajun Bai,Bin Li,Peinan Fan,Haiyan Lu,Pu Jia,Ye Zhao,Yujun Bai,Shixiang Wang,Kechun Liu,Meng Jin,Tai-Ping Fan,Xiaohui Zheng
Developmental epileptic encephalopathies (DEEs), including Dravet syndrome (DS), require antiseizure medications (ASMs) that balance efficacy with developmental safety. There is an urgent clinical need for novel therapeutic agents that combine potent anticonvulsant activity with developmental safety. β-Asarone, an active constituent of Acorus plants, has demonstrated antiepileptic potential, but its toxicities severely limit clinical application. Notably, β-asaronol, a hydroxylated metabolite of β-asarone, may exhibit improved safety; however, its pharmacological properties and therapeutic potential remain systematically unelucidated. To evaluate the developmental safety and antiseizure efficacy of β-asaronol for pediatric refractory epilepsy, developmental toxicity, antiseizure activity, and neuroprotective effects were systematically assessed using zebrafish models (Tg vmat2:GFP, Tg lfabp:EGFP, pentylenetetrazole (PTZ)-induced seizures, and scn1lab–/– mutants). Electrophysiology, molecular docking, and biomarker analyses elucidated mechanisms. β-Asaronol (5–150 μM) exhibited no significant developmental toxicity in zebrafish, with normal hatching rate, mortality, malformation rate, and no abnormalities in neurotoxicity or hepatotoxicity indicators. Its lethal concentration 50 (LC50) value was 3.5-fold higher than that of the parent compound β-asarone. It prolonged seizure latency, suppressed PTZ-induced hyperactivity (150 μM restoring baseline locomotion), and reduced neuronal apoptosis (lactate dehydrogenase (LDH)/c-fos normalization). In scn1lab–/– mutants, β-asaronol outperformed stiripentol and cannabidiol, reducing epileptiform discharges by about 93%. Mechanistically, β-asaronol potentiated γ-aminobutyric acid type A (GABAA) receptor currents (EC50 = 20.8 μM) via benzodiazepine-binding site interactions. β-Asaronol combines superior developmental safety with potent antiseizure efficacy, positioning it as a promising candidate for Dravet syndrome and refractory epilepsy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2022
·
IF 4.885
Jinmao Ma,Dongrun Wang,Wei Zhang,Xiangyu Wang,Xia Ma,Minsheng Liu,Quanlin Zhao,Lincheng Zhou,Sanxiang Sun,Zhengfang Ye
The host–guest β-cyclodextrin has attracted much attention for potential application in various fields due to its low price, natural pores, and functionality. Herein, we report a method using 1,4-phenylene diisocyanate (PPDI) to fabricate β-cyclodextrin (CD)-modified poly(chloromethyl styrene) (PCMS) resin, namely, PCMS@PPDI-CD. β-Cyclodextrin was utilized to improve the adsorption performance of PCMS, which was then applied to anchor β-cyclodextrin powder. The product exhibits excellent renewability and availability due to the application of PCMS. The adsorption performance was fully exerted by the combination of β-cyclodextrin and PCMS. SEM mapping, FTIR, TG , BET, and XPS analyses were conducted to explore the morphological and structural properties of PCMS@PPDI-CD. Adsorption experiments show that PCMS@PPDI-CD removed 100 % of TC and 75 % of Cu(Ⅱ), and the maximum adsorption capacity for Cu(Ⅱ) and TC reached 187.6 and 266.4 mg g −1 , respectively. After recycling six times, the adsorption ability of Cu(Ⅱ) and TC still achieved excellent removal performance. In addition, the chelation of the lone N pair, the hydrophobicity of the cavity, H-bonding, complexation, n-π interaction, and porous structure showed the possible mechanism. We used computational formulas to simulate the adsorption state and found that the resin is theoretically feasible. Most importantly, we propose a novel application of PCMS@PPDI-CD. This work shows that the resulting PCMS@PPDI-CD is highly efficient and can be easily recycled, making it an attractive resin for adsorption.
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiyue Xie,Haijun Wang,Li Zhang,Yuting Liu,Yaqin Chai,Yali Yuan,Ruo Yuan
The shortened distance between luminophor and coreactant can effectively enhance luminous efficiency in electrochemiluminescence (ECL) system owing to the improvement of electron transfer rate. Here, a novel sandwich-type ECL immunosensor based on N-(aminobutyl)-N-(ethylisoluminol) (ABEI) as luminophor was constructed by integrating hollow gold nanoparticles (HAuNPs), l -cysteine ( l -Cys) and ferrocene (Fc) in a complex for multiple signal amplification. The HAuNPs with large specific surface areas and superior conductivity were first used as scaffolds to load abundant coreactant l -Cys, aiming to further assemble catalyzer Fc stably. Through host-guest recognition, the β-cyclodextrin (β-CD) functionalized ABEI thus subsequently decorated on Fc, bringing in shortened distance between luminophor ABEI and coreactant l -Cys with high ECL signal. Moreover, the catalyzer Fc and HAuNPs could dramatically promote decomposition of another coreactant H 2 O 2 to produce abundant superoxide radical (O 2 ˉ ) and hydroxyl radical (OH ) for further signal amplification. Taking β2-microglobulin (β2 M) as model analyte, the developed immunosensor exhibited a superior performance for detection β2 M in the range of 1.0 × 10 −3 –1.0 × 10 3 ng mL −1 with a detection limit of 2.6 × 10 −1 pg mL −1 . The developed strategy realized solid-state ECL detection and multiple ECL signal amplification, providing a novel sensitive detection method for β2 M, which may show a great promise for the clinical application.
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Na Li,Huan Xia,Zifu Ni,Zewang Guo,Yang Song,Wenquan Huang,Yanbin Jiang,Wenyong Lou
A robust endoxylanase with high activity has great potential in biomass utilization. Here, the catalytic efficiency of the endoxylanase XynF derived from Herbinix hemicellulosilytica was improved by fusing a β-xylosidase xln-DT derived from Dictyoglomus thermophilum with linkers (L0 and L3) and the direction from xln-DT to XynF. The biochemical properties and application potential of chimeras towards corncob xylan were explored. The result showed that the chimeras Xln-L0-XynF and Xln-L3-XynF exhibited higher catalytic efficiency (31-fold and 20-fold enhancement, respectively), and maintained high stability in some harsh environments compared with parental enzymes. This might be attributed to the appropriate linkers and the direction which decrease the adverse interactions between the two modules. These two chimeras could hydrolyze corncob xylan to produce approximately 30% xylooligosaccharides with polymerization of 2–6. This work represents the promising xylanases for participating in XOS production from an abundant corncobs and promote us to modify glycoside hydrolase by fusion technology.
✓ 已验证
DOI
高影响因子
Arabian Journal of Chemistry
·
2017
·
IF 4.553
Parbeen Singh,Xiaohong Ren,Yaping He,Li Wu,Caifen Wang,Haiyan Li,Vikramjeet Singh,Jiwen Zhang
The fabrication of supramolecular host in combination biomolecules is an interesting idea in modern drug delivery for development of new polymer with advanced chemical and biological properties. Herein, hyper-crosslinked copolymer was fabricated from β-cyclodextrin (β-CD) and sialic acid (SA) monomers , which can be undoublty considered as a new class of copolymer. The as-synthesized copolymer has complexation properties, which could cover the drug within the structure and deliver to the site of action. The well-known inclusion capability of β-CD and targeted efficacy of SA made it more appropriate for targeted drug delivery. The copolymer was characterized using a wide range of spectroscopic and microscopic techniques such as synchrotron radiation based FTIR spectroscopy (SR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and powder X-ray diffraction (PXRD). The surface area and porosity were calculated by using Nitrogen adsorption method. Doxorubicin (Dox) was selected as a model drug to evaluate the loading efficiency and cellular penetration ability of the copolymer. The copolymer showed high adsorption towards Dox with no significant cytotoxic effects on HeLa cells as proved by cell viability assay. High cellular penetration of Dox loaded copolymer was also recorded by confocal microscopy when compared with free Dox in HeLa cells at 4 h of exposure. Thus, β-CD-SA copolymer could be a useful carrier for targeted drug delivery of cancer and has the potential for further investigation in viral and nervous disease due to the targeting ability of SA.
✓ 已验证
DOI
高影响因子
JOURNAL OF CELLULAR BIOCHEMISTRY
·
2019
·Vol. 120
·期 10
·17273-17282
·
IF 3.448
Min-yi Lv,Chuan-jian Shi,Fei-fei Pan,Jiang Shao,Lu Feng,Guoqin Chen,Caiwen Ou,Jin-fang Zhang,Wei-ming Fu
Consumption of dietary ellagitannins (ETs) has been proven to benefit multiple chronic health disorders including cancers and cardiovascular diseases. Urolithins, gut microbiota metabolites derived from ETs, are considered as the molecules responsible for these health effects. Previous studies have demonstrated that urolithins exhibit antiproliferative effects on prostate, breast, and colon cancers. However, as for hepatocellular carcinoma (HCC), it remains elusive. Herein, we aim to investigate the function of urolithin B (UB), a member of urolithins family, in HCC. The effects of UB on cell viability, cell cycle and apoptosis were evaluated in HCC cells, and we found UB could inhibit the proliferation of HCC cells, which resulted from cell cycle arrest and apoptosis. Furthermore, UB could increase phosphorylated β-catenin expression and block its translocation from nuclear to cytoplasm, thus inducing the inactivation of Wnt/β-catenin signaling. Using a xenograft mice model, UB was found to suppress tumor growth in vivo. In conclusion, our data demonstrated that UB could inhibit the proliferation of HCC cells in vitro and in vivo via inactivating Wnt/β-catenin signaling, suggesting UB could be a promising candidate in the development of anticancer drugs targeting HCC.
✓ 已验证
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
·
2022
·
IF 11.322
Dan Liu,Jiajun Qiu,Ru Xu,Junyu Liu,Jiayin Feng,Liping Ouyang,Shi Qian,Yuqin Qiao,Xuanyong Liu
Bacterial infections in wounds often delay the healing process which threaten human health and life. The development of biocompatible hydrogels with antibacterial activity and self-healing to achieve excellent wound closure effectiveness is highly desirable in clinical applications. Herein, a new class of highly stretchable, biocompatible, and antibacterial hydrogel dressings called BPP hydrogel is designed and prepared by chemically cross-linking β-CD with PEI and then physically cross-linking with PVA. The BPP hydrogel exhibits superior properties including excellent antibacterial properties against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli , good biocompatibility for mouse fibroblasts , mouse embryonic fibroblasts and human umbilical vein endothelial cells, outstanding deformability and self-healing performance to protect the wound completely even in special scenes such as pulling and bending. Besides, after damage, the broken BPP hydrogel could heal together, and the healed BPP hydrogel could be stretched to 150% breaking deformation. Furthermore, in vivo experiments further verify that BPP hydrogel has excellent sterilization and healing effect on infectious wounds. This work proves that BPP hydrogel would be a promising material for wound healing and has good prospect for clinical translation.
✓ 已验证
DOI
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2023
·
IF 3.9
Xiaoting Dong,Wenli Liu,Yuxuan Dong,Kun Wang,Kewei Li,Liujiao Bian
Metallo-β-lactamases (MβLs) are the primary mechanism of resistance to carbapenem antibiotics. To elucidate how MβLs have evolved with the introduction and use of antibiotics, the mutation and evolution of SMB-1 from Serratia marcescens were investigated in microbial evolution plates containing discontinuous meropenem (MEM) concentration gradients. The results revealed 2-point mutations, A242G and S257R; 1 double-site mutation, C240G/E258G; and 3 frameshift mutations , M5, M12, and M13, which are all missense mutations situated at the C-terminus. Compared with that of the wild-type (WT), the minimum inhibitory concentrations (MICs) of MEM for A242G, C240G/E258G, M5, M12, and M13 increased at least 120-fold, and that of S257R increased 8-fold. The catalytic efficiency k cat /K m increased by 365% and 647%, respectively. Concerning the structural changes, the structure at the active site changed from an ordered structure to an unordered conformation. Simultaneously, the flexibility of loop 1 was enhanced. These changes increased the volume of the active site cavity; thus, this was more conducive to exposing the Zn 2+ site, facilitating substrate binding and conversion to products. In A242G, structural changes in Gly-242 can be transmitted to the active region via a network of interactions between the side chains of Gly-242 and the amino acid side chains near the active pocket. Together, these results pointed to the process of persistent drug tolerance and resistance, the SMB-1 enzyme evolved into a more exquisite structure with increased flexibility and stability, and stronger hydrolysis activity via genetic mutations and structural changes.
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 47
·28397-28407
·
IF 3.119
Long Qin,Jiang Yi,Lai Xuefei,Liao Li,Xie Kenan,Xie Lu
Porous β-tricalcium phosphate (β-Ca3(PO4)2, β-TCP) scaffolds are widely applied in the field of bone tissue engineering due to their nontoxicity, degradability, biocompatibility, and osteoinductivity. However, poor compressive strength and a lack of antibacterial properties have hindered their clinical application. In order to address these disadvantages, graphene (G) and silver nanoparticles were introduced into β-TCP through a two-step method. In the synthesis process, G-β-TCP was prepared via an in situ synthesis method, and then silver nanoparticles and HAp particles were coated on the surface of the G-β-TCP scaffold in an orderly fashion using dopamine as a binder. From the results of characterization, when the content of graphene was 1 wt% of β-TCP, the G-β-TCP scaffold had the highest compression strength (127.25 MPa). And core–shell G-β-TCP-Ag-HAp not only had reduced cytotoxicity via the continuous release of Ag+, but it also achieved long-term antibacterial properties. Besides, the material still showed good cell activity and proliferation.
✓ 已验证
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Rijian Song,Xuan Mao,Shunqing Tang
Here we prepared a low-degree-sulfated κ/β-carrageenan oligosaccharide (L-DS-KOS) by DMSO-methanol desulfation method, and fabricated a sponge dressing for the wound healing of diabetic rats. The immunomodulatory effects of L-DS-KOS on M1-like macrophages were evaluated. Results showed that L-DS-KOS could effectively promote the secretion of anti-inflammatory factors and accelerate polarization of LPS-activated macrophages from M1 to M2 type. The gross examination result showed that the sponge dressing with the mass ratio of L-DS-KOS: collagen = 3: 7 could effectively accelerate the repair process of the full-thickness excisional wound in diabetic rats; and H&E and Masson staining results disclosed that the L-DS-KOS/collagen dressing could better shorten the inflammation period of the wound site, and improve the process of collagen deposition and epithelial formation, thereby promote the repair of skin wounds in diabetic rats. These results demonstrate that L-DS-KOS has potential to be used as a new type of immunomodulatory biomaterial for diabetic wound dressings.
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 18
·4188
·
IF 3.267
Ce Cheng,Yi Gao,Zhihua Wu,Jinyu Miao,Hongxia Gao,Li Ma,Liqiang Zou,Shengfeng Peng,Chengmei Liu,Wei Liu
β-carotene is a promising natural active ingredient for optimum human health. However, the insolubility in water, low oral bioavailability, and instability in oxygen, heat, and light are key factors to limit its application as incorporation into functional foods. Therefore, gliadin nanoparticles (GNPs) Pickering emulgels were chosen as food-grade β-carotene delivery systems. The objectives of the present study were to investigate the influence of GNPs concentration on the rheological properties, stability, and simulated gastrointestinal fate of β-carotene of Pickering emulgels. The formulations of Pickering emulgels at low GNPs concentration had better fluidity, whereas at high GNPs concentration, they had stronger gel structures. Furthermore, the thermal stability of β-carotene loaded in Pickering emulgels after two pasteurization treatments was significantly improved with the increase of GNPs concentration. The Pickering emulgels stabilized with higher GNPs concentration could improve the protection and bioaccessibility of β-carotene after different storage conditions. This study demonstrated the tremendous potential of GNPs Pickering emulgels to carry β-carotene.Keywords:β-carotene;gliadin;Pickering emulgels;rheological property;stability;bioavailability
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）