JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Zhao Mengfei,Zhang Jichuan,Guan Meng,Li Xuanhan,Sun Huating,Mao Kuirong,Tan Huizhu,Zhang Yijia,Meng Xandi,Cong Xiuxiu,Xin Yanbao,Wang Haorui,Yu MeiLing,Chen Si,Zhou Hongxv,Wang Xinhe,Yang Yong-Guang,Sun Tianmeng,Zhang Yuning
Immune checkpoint blockade (ICB) remains limited by the immunosuppressive tumor microenvironment (TME) and by the difficulty of coordinating therapeutic agents that act in distinct tumor compartments. In particular, checkpoint antibodies must function in the tumor stroma, whereas TME-remodeling small molecules often require intracellular access, creating an extracellular–intracellular delivery mismatch that conventional carriers rarely resolve. Here, we develop an acid-gated, esterase-amplified AND-gated dendritic nanogel (DNG) for the spatiotemporally sequential codelivery of anti-PD-1 antibody (aPD-1) and the PPARδ antagonist GSK3787. The DNG comprises a PEG corona for reversible aPD-1 adsorption and a hydrophobic dendritic core for GSK3787 encapsulation. Under the combined cues of tumor acidity and esterase activity, the nanogel undergoes progressive dissociation, triggering preferential extracellular release of aPD-1 in the tumor stroma, followed by tumor-cell internalization of the GSK3787-loaded core for intracellular drug action. This AND-gated design enables spatially resolved pharmacology rather than simple codelivery. In a melanoma model, DNG@(GSK3787 + aPD-1) markedly enhances antitumor efficacy, thereby promotes CD8⁺ T cell infiltration, and elicits systemic antitumor immune responses. Mechanistically, we identify a tumor cell-centric mode of immune remodeling and further show that the dendritic core actively governs not only small-molecule encapsulation, but also antibody retention and triggered release. Together, this work establishes a materials strategy for overcoming extracellular–intracellular delivery mismatch in combination immunotherapy. Graphical AND-gated dual-responsive nanogel enables spatiotemporally sequential extracellular delivery of aPD-1 and intracellular delivery of GSK3787, thereby overcoming the extracellular–intracellular delivery mismatch and synergistically remodeling the tumor immune microenvironment for enhanced cancer immunotherapy.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
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IF 4.6
Ziyu Zhang,Yanlin Li,Rong Fang,Hongfang Zhang
Gold nanorods with different aspect ratios were synthesized by pH regulation. • A DNA three-way junction was employed for target recognition and AuNRs introduction. • A highly sensitive amperometric biosensor was developed for MUC-1 detection. Mucin 1 (MUC-1) is a valuable tumor-associated transmembrane glycoprotein. Electrochemical biosensor is a convenient tool for the quantitative detection of MUC-1 in biological samples. In this research, a new MUC-1 biosensor is developed based on gold nanorods conjugated with DNA three-way junction. The sandwiched biosensor is fabricated by covalent anchoring the anti-MUC-1 antibody on an electrode surface which is modified with the nanocomposite of silver nanoparticles and chitosan. In the presence of MUC-1, gold nanorods can be specifically introduced onto the sensor surface because of one aptamer tail of the DNA three-way junction, which is conjugated with gold nanorods via two thiol terminals. Then, the reductive current of H 2 O 2 monitored by amperometry increases drastically with the increase of MUC-1 concentration. Under the optimized conditions, the biosensor exhibited a linear response over the range of 100 fg·mL −1 to 1 μg·mL −1 , with a low detection limit of 54.3 fg·mL −1 . The biosensor showed good reproducibility, with an RSD of 4.7% for the response of five independently fabricated sensors. The detection recovery in spiked serum samples ranges from 87.1% to 110.0%, demonstrating the application potential of the proposed strategy for ultrasensitive electrochemical detection of MUC-1 in biological samples. Download: Download high-res image (193KB) Download: Download full-size image
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Cell Communication and Signaling
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2026
·
IF 11.6
Liu Fang,Han Chao,Xiao Yi
Histone lactylation is a recently identified epigenetic modification that directly couples cellular metabolic state with gene expression. Whether lactate affects host resistance to pathogens via histone lactylation remains unclear. Here, we found that infection with Pseudomonas aeruginosa PA14 significantly increased lactate levels in Caenorhabditis elegans . Exogenous sodium lactate (NAL) also raised lactate levels and reduced worm resistance to the extracellular bacteria P. aeruginosa and Enterococcus faecalis , but had no detectable effect on the intracellular bacteria Salmonella and Listeria monocytogenes . Lowering lactate levels with the lactate dehydrogenase inhibitor Oxamate enhanced worm resistance to infection. Genetic screening showed that the regulatory effects of NAL and Oxamate on immunity were dependent on SKN-1. Similar results were also observed with pyruvate and the pyruvate dehydrogenase kinase inhibitor dichloroacetate (DCA) treatment. Mechanistic studies showed that, among the histone lactylation sites examined, elevated lactate promoted lactylation most strongly at H4K16 (H4K16la). ChIP‑qPCR results showed increased H4K16la enrichment at the skn-1 promoter upon lactate elevation. This led to increased activity of the transcription factor SKN-1, as evidenced by increased SKN-1 nuclear translocation, upregulation of downstream genes ( skn-1 , gst-4 , gst-7 , gst-10 , gcs-1 ), and enhanced GST-4::GFP fluorescence. However, the addition of NAL caused excessive GST-4::GFP fluorescence, indicating SKN-1 overactivation. This also led to suppression of the antimicrobial peptide IRG-1 and increased bacterial accumulation in the gut. In summary, this study provides evidence that lactate suppresses innate immunity in C. elegans , likely through H4K16 lactylation-mediated overactivation of SKN-1. These findings suggest that lactate may act as a negative regulator of innate immunity in an invertebrate model and provide a basis for future studies on targeting lactate metabolism or histone lactylation in infectious diseases.
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BIOORGANIC CHEMISTRY
·
2026
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IF 5.1
Sheng-Li Wu,Xiao-Feng He,Pianchou Gongpan,Xiao-Yan Huang,Chang-An Geng
DCA stimulated GLP-1 secretion in STC-1 and NCI-H716 cells. • DCA decreased fasting blood glucose levels by promoting GLP-1 secretion in db/db mice. • DCA ameliorated glucose intolerance and insulin resistance and lowered serum TC levels. • DCA promoted GLP-1 secretion through specific activation on TGR5/PKA/MEK/ERK pathway. Type 2 diabetes mellitus (T2DM) is a heterogeneous metabolic disorder characterized by insulin resistance and progressive β cell dysfunction. Deoxycalyxin A (DCA), a unique diarylheptanoid-chalcone hybrid isolated from Alpinia katsumadai , was identified as a novel stimulator of GLP 1 secretion in both STC-1 (EC 50 : 10.7 μM) and NCI-H716 (EC 50 : 13.4 μM) cells. Oral administration of DCA (40 and 80 mg/kg) in db/db mice significantly reduced fasting blood glucose, ameliorated glucose intolerance and insulin resistance by enhancing endogenous GLP-1 secretion, and lowered the total serum cholesterol levels. Mechanistic study demonstrated that DCA identify the TGR5 receptor, which induces a significant increase in intracellular cyclic AMP (cAMP) levels, which activates PKA with subsequent sequential phosphorylation of MEK1/2 and ERK activation, ultimately enhancing GLP-1 secretion. Our findings identify DCA as a novel TGR5 agonist to stimulate GLP-1 secretion via the TGR5/PKA/MEK/ERK pathway, which has the potential to be developed as a promising antidiabetic lead with a distinct mechanism of action. DCA, a unique diarylheptanoid-chalcone from Alpinia katsumadai , is a novel TGR5 agonist to stimulate GLP-1 secretion via PKA/MEK/ERK pathway, which has the potential to be developed as a promising antidiabetic candidate. Download: Download high-res image (123KB) Download: Download full-size image
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yuchen Zhu,Jian Xu,Di Zhang,Aodong Zhang,Boxin Chen,Bin Zhao,Xuhong Qian,Ya Cheng,Fei Feng
Background The gas chromatography column is one of the key components of the gas chromatograph and typically be miniaturized using micro-electro-mechanical system (MEMS) technology. Due to the limited area of the Si wafer, the column length of micro gas chromatographic column (μGCC) is usually much smaller than that of commercial chromatographic columns. Therefore, it is always difficult to use μGCCs to separate small molecule gas components such as light hydrocarbons. More importantly, the heterogeneous microchannel surface formed by silicon glass bonding causes uneven stationary phase coating, further preventing the improvement of separation performance. Results In this paper, a novel all-glass based μGCC with 2 m length for the separation of light hydrocarbons is proposed. The microchannels of the μGCC were directly prepared in the glass substrate by ultrafast laser assisted chemical etching (ULAE). The all-glass microchannels make the coating of the hydrophilic metal-organic frameworks (MOFs) stationary phase continuously because of the homogeneous material composition. Therefore, a widely used copper based hydrophilic MOFs HKUST-1 was used as stationary phase for coating and testing. The test results show that the μGCC which is an open tubular column can realize the baseline separation of light hydrocarbons at 100 °C. And the resolution of difficult separated compounds, methane and ethane, can reach 12.98, which is 201.86 % higher than the silica-based monolithic capillary column in the relevant research. The resolution of ethane and ethylene reaches 6.81 at 120 °C. Significance The μGCC fabricated by ULAE method is composed of all-glass and has the uniform stationary phase coating because of the homogeneous microchannel surface which greatly improve the separation performance, resulting in a large resolution for methane and ethane. The all-glass μGCC has broad application prospects in light hydrocarbon separation.
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GOLD BULLETIN
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2023
·
IF 2.2
Luan Wei,Zheng Meiyun,Yang Youlin,Chen Yi,Zhang Xiahui,Zhu Lingping,Lin Chenxiao
Since their discovery, graphene nanocomposites have attracted much attention for their potential use in many biological applications. Herein, we examined the highly reduced graphene oxide (HRGO) and gold nanomaterial (AuNM)-based (HRGO/Au@AP) nanocomposite for ovarian cancer and apoptosis-inducing abilities, the nanomaterials’ anticancer activities against human ovarian cancer cell lines (SKOV3 and A2780). HRGO was functionalized with the 1-aminopyridine (AP) as a potential stabilizing agent to improve the sample’s solubility and bioavailability. The surface morphology and structure of the nanocomposites were examined by high-resolution transmission electron microscopy. The results of an anticancer study comparing HRGO, HRGO/Au, and HRGO/Au@AP nanocomposites showed a greater capacity to induce apoptosis, the apoptosis assays (AO-EB, DAPI, and Annexin V-FITC/PI staining) and reactive oxygen species (ROS) measurements on SKOV3 and A2780 cells. This data suggests that HRGO/Au@AP promotes potent apoptosis in human ovarian cancer cells.
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CHINESE JOURNAL OF PHYSICS
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2023
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IF 5
Congliang Chen,Yujie Yang,Yingming Zhang,Dongyang Chen,Yuting Meng
High-entropy oxides are composed of multi-component composite oxides. High-entropy oxides show some fascinating properties attributed to complex elements. It is generally believed that a considerable level of disorder in the high entropy oxides leads to the random arrangement of metal cations within the lattice. Interestingly, in spinel ferrites, each ion has distinct inclination towards a specific position. In this work, four different high entropy spinel oxides with the same element type but different ratios are designed and synthetized by adjusting the concentration of nonmagnetic ions (Zn 2+ ) in the spinel structure which obviously tends to tetrahedral positions. The findings reveal that the Zn 2+ still shows the discernible preference for a tetrahedral orientation in the highly disordered crystal. In addition, the variations of the magnetic properties of high-entropy oxides with the content of Zn 2+ are analyzed. The magnetic properties of samples demonstrate that they have elevated adjustability. In addition, with the increase of Zn 2+ content, the value of M s fluctuates obviously, which proves that the cations in the high entropy spinel oxide are not completely randomly distributed.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Xiaofu Tang,Yan Liang,Shengjing Guan,Lichun Xu,Meiyu Zhu,Wenmin Guo,Lihua Zou,Changlong Sun,Fuzhou Chen
Metallic phase molybdenum disulfide (1 T MoS 2 ) is one of the most promising anode materials for lithium-ion batteries. The 1 T MoS 2 is metastable and synthesis of 1 T MoS 2 without harsh and hazardous conditions is remain challenging. In this work, stable 1 T MoS 2 is prepared by the hydrothermal method utilizing the boron nitride co-doping graphite (BNG) nanosheets as substrates. The doping strategy changed the electron states in the graphite realizing the electron redistribution when combined with MoS 2 . The BNG nanosheets act as the electron donor stabilizing the 1 T MoS 2 and guiding the vertical growth of the MoS 2 without agglomeration. As a result, the as-prepared BNG-MoS 2 presents an outstanding lithium storage performance of 1056.3 mAh/g after 500 cycles and 248.3 mAh/g of capacity at a high rate of 20 A/g after 2000 cycles. Electrochemical measurements indicate the enhanced lithium storage kinetics is derived from the elevated electron conductivity and lithium-ion migration. The DFT calculation further confirms the origin of the phase transition and fast lithium storage kinetics is the electron redistribution at the interface of BNG-MoS 2 . This novel strategy gives a new perspective on the synthesis of stable 1 T phase MoS 2 for advanced anode materials.
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Aging-US
·
2023
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IF 5.2
Huahua Li,Jin Zheng,Yacen Wu,Hong Zhou,Suli Zeng,Quanqing Li
Backgrounds: High glucose (HG) caused oxidative stress and mitochondrial dysfunction, resulting in insulin resistance in podocytes, a key mechanism of diabetic nephropathy. Dendrobium officinale polysaccharide (DOP) was able to improve insulin resistance and antioxidant capability. Objective: The purpose of this study is to explore the mechanism by which DOP decreases the podocyte injury induced by HG. Methods: MPC5 cells were treated with HG, DOP, and IRS-1/2 inhibitor NT157. Afterwards, glucose consumption, generations of ROS and MDA were measured using the detection kits. Mitophagy was monitored using both MtphagTracyker and LysoTracker. The mitochondrial membrane potential was evaluated by JC-1 staining. DOP was also used in a mouse model of diabetes, with the measurements of urine albumin, blood creatinine and blood urea nitrogen. Results: Treatment with DOP suppressed the HG-induced reduction of glucose consumption, the phosphorylation of IRS-1 (phospho Y632), AKT (phospho Ser473 and Thr308) and Nephrin. In addition, HG-induced augment of ROS and MDA, formation of γ-H2A.X foci and translocation of AKT to nucleus were inhibited by DOP. DOP enhanced mitophagy, which was associated with decreased mitochondrial membrane potential and ROS production. DOP conferred protective effect on podocyte in the diabetic mouse by reducing the albumin/creatinine ratio and blood urea nitrogen, and restoring Nephrin expression in podocytes. Conclusions: DOP alleviates HG-induced podocyte injuryby regulating IRS-1/AKT signal and promoting mitophagy.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 18
·13682
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IF 5.6
Shiyao Wang,Dongfa Lin,Jiaofei Cao,Liping Wang
Animal studies have proven that 1-acetyl-5-phenyl-1H-pyrrol-3-yl acetate (APPA) is a powerful antioxidant as a novel aldose reductase inhibitor independently synthesized by our laboratory; however, there is no current information on APPA’s anti-aging mechanism. Therefore, this study examined the impact and mechanism of APPA’s anti-aging and anti-oxidation capacity using theCaenorhabditis elegansmodel. The results demonstrated that APPA increasesC. elegans’ longevity without affecting the typical metabolism ofEscherichia coliOP50 (OP50). APPA also had a non-toxic effect onC. elegans, increased locomotor ability, decreased the levels of reactive oxygen species, lipofuscin, and fat, and increased anti-stress capacity. QRT-PCR analysis further revealed that APPA upregulated the expression of antioxidant genes, includingsod-3,gst-4, andhsp-16.2, and the critical downstream transcription factors,daf-16,skn-1, andhsf-1of the insulin/insulin-like growth factor (IGF) receptor,daf-2. In addition,fat-6andnhr-80were upregulated. However, the APPA’s life-prolonging effects were absent on thedaf-2,daf-16,skn-1, andhsf-1mutants implying that the APPA’s life-prolonging mechanism depends on the insulin/IGF-1 signaling system. The transcriptome sequencing also revealed that the mitochondrial route was also strongly associated with the APPA life extension, consistent withmev-1andisp-1mutant life assays. These findings aid in the investigation of APPA’s longevity extension mechanism.Keywords:APPA;lifespan;stress resistant;insulin/IGF-1;Caenorhabditis elegans
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SOLID STATE SCIENCES
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2023
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IF 3.5
Qiqi Huang,Fuxiao Zhu,Feiyan Xiao,Gongliang Zhang,Hongman Hou,Jingran Bi,Shuang Yan,Hongshun Hao
In recent years, using external mechanical energy to solve environmental pollution problems has gradually become a promising strategy, such as piezoelectric catalysis and piezoelectric photocatalysis . In this paper, a series of HKUST-1/g-C 3 N 4 composite materials were successfully synthesized by the combination of g-C 3 N 4 with S-vacancy and porous HKUST-1 semiconductor. Under visible light irradiation and ultrasonic action, the catalytic activity of HKUST-1/g-C 3 N 4 on RhB degradation was investigated. When the mass ratio of HKUST-1 to g-C 3 N 4 is 1:0.3 during the process of piezoelectric photocatalysis, the as-prepared HKUST-1/g-C 3 N 4 composite had the best RhB degradation performance up to 94.42%, which is about 2.83 times that of visible light and 3.56 times that of ultrasonic waves. According to the result of the trapping experiment, the process of piezo-photocatalytic reaction could produce the number of reactive oxygen species (ROS), and the hydroxyl radical (•OH) was crucial in the breakdown of pollutants.
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ACS Infectious Diseases
·
2023
·
IF 5.3
Zewen Wen,Cong Wang,Bing Bai,Xinyi Cao,Kewei Fan,Chunyou Hu,Peiyu Li,Qiwen Deng,Zhijian Yu
Streptococcus agalactiae is the major cause of invasive neonatal infections and is a recognized pathogen associated with various diseases in nonpregnant adults. The emergence and spread of antibiotic-resistant S. agalactiae necessitate the development of a novel antibacterial agent. Here, the potential antibacterial activities and mechanisms of ginkgolic acid C15:1 (GA (15:1)) from Ginkgo biloba against clinical S. agalactiae are characterized. The MIC50 and MIC90 values for GA (15:1) against 72 clinical S. agalactiae isolates were 6.25 and 12.5 μM, respectively. GA (15:1) showed a strong bactericidal effect against both planktonic bacteria and bacteria embedded in biofilms as well as significant effectiveness in suppressing the growth of S. agalactiae biofilms. Moreover, GA (15:1) possesses intracellular antibacterial activity and could significantly decrease the bacterial burden in the intraperitoneal infection model of S. agalactiae. Mechanistic studies showed that GA (15:1) triggers membrane damage of S. agalactiae through a unique dual-targeting mechanism of action (MoA). First, GA (15:1) targets phospholipids in the bacterial cytoplasmic membrane. Second, by using mass-spectrometry-based drug affinity responsive target stability (DARTS) and molecular docking, lipoprotein signaling peptidase II (lspA) was identified as a target protein of GA (15:1), whose role is crucial for maintaining bacterial membrane depolarization and permeabilization. Our findings suggest a potential therapeutic strategy for developing GA (15:1) to combat S. agalactiae infections.
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