Nano Research
·
2024
·Vol. 17
·期 7
·5863-5871
·
IF 9.5
Ke Yanjing,Zhang Jiaxing,Peng Xin,Zhang Zhiyi,Wang Xu,Qi Wei,Wang Mengfan
Recently, many efforts have been dedicated to construct artificial catalysts with enzyme-like activity. However, it is still a big challenge to endow artificial catalysts with specific substrate selectivity. In this study, we developed a facile strategy to construct a MIL-53(Fe)-based nanocatalyst with designable selectivity in the degradation of oxytetracycline (OTC). Through the Fe–O–P conjunction, oxytetracycline aptamer (OA) can be easily anchored on MIL-53(Fe) to provide the specific site for OTC binding. We verified that the obtained MIL-53(Fe)-Apt nanocatalyst displayed enhanced catalytic ability in the degradation of OTC, whereas obvious suppression toward other substrate analogues. This performance therefore brings about an anticipated selectivity toward OTC. Moreover, we highlighted that the configuration of aptamers on MIL-53(Fe) can be modulated through varying conjunction mode. Structure–function analysis revealed that aptamer configuration affects the local concentration of substrate around catalytic site, which thus decides the catalytic performance toward OTC. This work presented a facile and promising strategy for developing artificial catalysts with designable selectivity.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-18
·
IF 14.7
Qiu Sheng,Wu Qinan,Wang Hao,Liu Dongfang,Chen Chen,Zhu Zhiming,Zheng Hongting,Yang Gangyi,Li Ling,Yang Mengliu
Zinc-alpha2-glycoprotein (AZGP1) has been implicated in peripheral metabolism; however, its role in regulating energy metabolism in the brain, particularly in POMC neurons, remains unknown. Here, we show that AZGP1 in POMC neurons plays a crucial role in controlling whole-body metabolism. POMC neuron-specific overexpression of Azgp1 under high-fat diet conditions reduces energy intake, raises energy expenditure, elevates peripheral tissue leptin and insulin sensitivity, alleviates liver steatosis, and promotes adipose tissue browning. Conversely, mice with inducible deletion of Azgp1 in POMC neurons exhibit the opposite metabolic phenotypes, showing increased susceptibility to diet-induced obesity. Notably, an increase in AZGP1 signaling in the hypothalamus elevates STAT3 phosphorylation and increases POMC neuron excitability. Mechanistically, AZGP1 enhances leptin-JAK2-STAT3 signaling by interacting with acylglycerol kinase (AGK) to block its ubiquitination degradation. Collectively, these results suggest that AZGP1 plays a crucial role in regulating energy homeostasis and glucose/lipid metabolism by acting on hypothalamic POMC neurons.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-10
·
IF 14.7
Fang Jinjie,Wang Haiyong,Dang Qian,Wang Hao,Wang Xingdong,Pei Jiajing,Xu Zhiyuan,Chen Chengjin,Zhu Wei,Li Hui,Yan Yushan,Zhuang Zhongbin
Hydroxide exchange membrane fuel cells (HEMFCs) have the advantages of using cost-effective materials, but hindered by the sluggish anodic hydrogen oxidation reaction (HOR) kinetics. Here, we report an atomically dispersed Ir on Mo 2 C nanoparticles supported on carbon (Ir SA -Mo 2 C/C) as highly active and stable HOR catalysts. The specific exchange current density of Ir SA -Mo 2 C/C is 4.1 mA cm −2 ECSA , which is 10 times that of Ir/C. Negligible decay is observed after 30,000-cycle accelerated stability test. Theoretical calculations suggest the high HOR activity is attributed to the unique Mo 2 C substrate, which makes the Ir sites with optimized H binding and also provides enhanced OH binding sites. By using a low loading (0.05 mg Ir cm −2 ) of Ir SA -Mo 2 C/C as anode, the fabricated HEMFC can deliver a high peak power density of 1.64 W cm −2 . This work illustrates that atomically dispersed precious metal on carbides may be a promising strategy for high performance HEMFCs.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Zhang Jinlong,White Jason C.,Lowry Gregory V.,He Jinglei,Yu Xuefeng,Yan Chuanhao,Dong Liang,Tao Shu,Wang Xilong
Enzyme-catalyzed biodegradation is an emerging green strategy for environmental remediation, although challenged by high cost and poor robustness. Herein, natural biopolymer (cellulose)-derived hydrogels concurrently doped with β-cyclodextrin and montmorillonite nanosheets that are synthesized in one-step demonstrate exceptional pollutant affinity and mechanical strength. Laccase is then stably and effectively assembled onto the hydrogels by a facile strategy based on charge-assisted H-bonding, which can be extended to other enzymes. The advanced laccase-assembled hydrogels display excellent stability and increased degradation activity achieved by strong substrate capture and rapid electron transfer. The laccase-assembled hydrogels exhibit significantly improved removal (62-fold) and degradation (52-fold) performance compared to free laccase for diverse organic pollutants (e.g., polycyclic aromatic hydrocarbons) in real wastewater. This enhanced performance is maintained despite the presence of heavy metals, other organic chemicals or dissolved organic matter. This work provides a practical strategy for designing an advanced and sustainable biodegradation tool for environmental remediation.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-20
·
IF 14.7
Ouyang Boshu,Shan Caihua,Shen Shun,Dai Xinnan,Chen Qingwang,Su Xiaomin,Cao Yongbin,Qin Xifeng,He Ying,Wang Siyu,Xu Ruizhe,Hu Ruining,Shi Leming,Lu Tun,Yang Wuli,Peng Shaojun,Zhang Jun,Wang Jianxin,Li Dongsheng,Pang Zhiqing
Due to low success rates and long cycles of traditional drug development, the clinical tendency is to apply omics techniques to reveal patient-level disease characteristics and individualized responses to treatment. However, the heterogeneous form of data and uneven distribution of targets make drug discovery and precision medicine a non-trivial task. This study takes pyroptosis therapy for triple-negative breast cancer (TNBC) as a paradigm and uses data mining of a large TNBC cohort and drug databases to establish a biofactor-regulated neural network for rapidly screening and optimizing compound pyroptosis drug pairs. Subsequently, biomimetic nanococrystals are prepared using the preferred combination of mitoxantrone and gambogic acid for rational drug delivery. The unique mechanism of obtained nanococrystals regulating pyroptosis genes through ribosomal stress and triggering pyroptosis cascade immune effects are revealed in TNBC models. In this work, a target omics-based intelligent compound drug discovery framework explores an innovative drug development paradigm, which repurposes existing drugs and enables precise treatment of refractory diseases.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Zhang Xiaoya,Wang Bing,Luo Yiting,Ding Chuan-Fan,Yan Yinghua
The elucidation of disease pathogenesis can be achieved by analyzing the low-abundance phosphopeptides in organisms. Herein, we developed a novel and easy-to-prepare polymer-coated nanomaterial. By improving the hydrophilicity and spatial conformation of the material, we effectively enhanced the adsorption of phosphopeptides and demonstrated excellent enrichment properties. The material was able to successfully enrich the phosphopeptides in only 1 min. Meanwhile, the material has high selectivity (1:2000), good loading capacity (100 μg/mg), excellent sensitivity (0.5 fmol), and great acid and alkali resistance. In addition, the material was applied to real samples, and 70 phosphopeptides were enriched from the serum of Parkinson’s disease (PD) patients and 67 phosphopeptides were enriched from the serum of normal controls. Sequences Logo showed that PD is probably associated with threonine, glutamate, serine, and glutamine. Finally, gene ontology (GO) analysis was performed on phosphopeptides enriched in PD patients’ serum. The results showed that PD patients expressed abnormal expression of the cholesterol metabolic process and cell–matrix adhesion in the biological process (BP), endoplasmic reticulum and lipoprotein in the cellular component (CC), and heparin-binding, lipid-binding, and receptor-binding in the molecular function (MF) as compared with normal individuals. All the experiments indicate that the nanomaterials have great potential in proteomics studies. Graphical Abstract
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Wang Yi,Wang Shuo,Fu Yunfan,Sang Jiaqi,Wei Pengfei,Li Rongtan,Gao Dunfeng,Wang Guoxiong,Bao Xinhe
Renewable energy-driven electrocatalytic nitrate reduction reaction presents a low-carbon and sustainable route for ammonia synthesis under mild conditions. Yet, the practical application of this process is currently hindered by unsatisfactory electrocatalytic activity and long-term stability. Herein we achieve high-rate ammonia electrosynthesis using a stable amorphous/crystalline dual-phase Cu catalyst. The ammonia partial current density and formation rate reach 3.33 ± 0.005 A cm −2 and 15.5 ± 0.02 mmol h −1 cm −2 at a low cell voltage of 2.6 ± 0.01 V, respectively. Remarkably, the dual-phase Cu catalyst can maintain stable ammonia production with a Faradaic efficiency of around 90% at a high current density of 1.5 A cm −2 for up to 300 h. A scale-up demonstration with an electrode size of 100 cm 2 achieves an ammonia formation rate as high as 11.9 ± 0.5 g h −1 at a total current of 160 A. The impressive electrocatalytic performance is ascribed to the presence of stable amorphous Cu domains which promote the adsorption and hydrogenation of nitrogen-containing intermediates, thus improving reaction kinetics for ammonia formation. This work underscores the importance of stabilizing metastable amorphous structures for improving electrocatalytic reactivity and long-term stability.
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Nano Research
·
2024
·
IF 9.5
Huang Danru,Wu Lin,Kang Qi,Shen Zhiyong,Huang Qiaosheng,Lin Wenjie,Pei Fei,Huang Yunhui
Solid-state polymer electrolytes (SPEs) are candidate schemes for meeting the safety and energy density needs of advanced lithium-based battery because of their improved mechanical and electrochemical stability compared to traditional liquid electrolytes. However, low ionic conductivity and side reactions occurring in traditional high-voltage lithium metal batteries (LMBs) hinder their practical applications. Here, amino-modified metal-organic frameworks (UiO-66-NH 2 ) with abundant defects as multifunctional fillers in the polyurethane based SPEs achieve the collaborative promotion of the mechanical strength and room temperature ionic conductivity. The surface modified amino groups serve as anchoring points for oxygen atoms of polymer chains, forming a firmly hydrogen-bond interface with polycarbonate-based polyurethane frameworks. The rich interfaces between UiO-66-NH 2 and polymers dramatically decrease the crystallization of polymer chains and reduce ion transport impedance, which markedly boosted the ionic conductivity to 2.1 × 10 −4 S·cm −1 with a high Li + transference numbers of 0.71. As a result, LiFePO 4 ∣SPEs∣Li cells exhibit prominent cyclability for 700 cycles under 0.5 C with 96.5% capacity retention. The LiNi 0.6 Co 0.2 Mn 0.2 O 2 (NCM622)∣SPEs∣Li cells deliver excellent long-term lifespan for 260 cycles with a high capacity retention of 91.9% and high average Coulombic efficiency (98.5%) under ambient conditions. This simple and effective hybrid SPE design strategy sheds a milestone significance light for high-voltage Li-metal batteries.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-14
·
IF 5.3
Liu Yansheng,Sun Yukun,Jia Xiaobo,Zhou Jin,Li Kang,Li Zhaoxu,Wang Guofu
A method for detecting methamphetamine (MET), ketamine (KET), and morphine (MOP) molecules is presented using a reusable substrate based on SERS. The SERS substrate was prepared by etching the Au/Ag alloy film to synthesize a nanoporous Au membrane (AuNPM). By optimizing the preparation conditions and using rhodamine 6G (R6G) as an analyte, the AuNPM exhibited good SERS performance with a limit of detection (LOD) of 10 −9 mol L −1 . A competitive immunoassay category has been applied to the detection of MET, KET, and MOP. The MET, KET, and MOP antigens were functionalized on the surface of the AuNPM to specifically bind to the related drug antibodies. The Au nanoparticles (AuNPs) modified with 4-mercaptobenzoic acid (4-MBA) and antibodies against MET, KET, and MOP were used as nanotags. The 4-MBA served as the reporting molecule and drug antibodies were used to bind to free drug molecules in the target solution. The mixture of nanotags and target solution was dropped onto the antigen-modified AuNPM (antigen/AuNPM), and the free nanotags bind to the antigen/AuNPM. By comparing the SERS intensity of 4-MBA with the presence or absence of drug molecules, the drugs were qualitatively and quantitatively identified. Through this category, the LODs for detecting MET, KET, and MOP were 0.1, 1, and 1 ng mL −1 , respectively. This study proposes an effective method for constructing SERS-based detection of drug molecules with good potential for practical applications. Graphical abstract
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Chen Zipeng,Zhang Qian,Ding Liping,Lv Guangxin,Liu Tianji,Yang Zhengwei,Jiang Yi,Li Longnan,Li Wei,Ding Feng,Xu Weilin,Zhu Jia,Zhu Bin
Infrared (IR)-transparent radiative cooling textiles show great promise for achieving personal thermal comfort and reducing energy consumption. However, besides a few synthetic fiber materials proposed as IR-transparent textiles, traditional textile materials used to achieve IR transparency have not been realized, impeding large-scale practical applications. Here, based on a common textile material Nylon 6 (PA6), we design a high drawing process with rapid solvent evaporation to achieve IR-transparent PA6 textiles. By altering the chain conformations and crystal structures, the molecular vibrations in the IR region (IR absorption) of PA6 can be significantly weakened. Meanwhile, this process also tailors the fiber to the nanoscale and minimizes IR reflection. Consequently, a human body covered by our textile can stay 2.1 °C cooler than with cotton, corresponding to ~20% indoor energy savings in cooling. We expect that our work offers an innovative pathway to regulate IR radiation for personal thermal management.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-8
·
IF 5.3
Ou Dan,Yan Huixian,Chen Zuanguang
Based on the electrochemical impedance method, a marker-free biosensor with aptamer as a biometric element was developed for the determination of doxorubicin (DOX). By combining aptamer with rigid tetrahedral DNA nanostructures (TDNs) and fixing them on the surface of gold electrode (GE) as biometric elements, the density and directivity of surface nanoprobes improved, and DOX was captured with high sensitivity and specificity. DOX was captured by immobilized aptamers on the GE, which inhibited electron transfer between the GE and [Fe(CN)6] 3− / 4− in solution, resulting in a change in electrochemical impedance. When the DOX concentration was between 10.0 and 100.0 nM, the aptasensor showed a linear relationship with charge transfer resistance, the relative standard deviation (RSD) ranged from 3.6 to 5.9%, and the detection limit (LOD) was 3.0 nM. This technique offered a successful performance for the determination of the target analyte in serum samples with recovery in the range 97.0 to 99.6% and RSD ranged from 4.8 to 6.5%. This method displayed the advantages of fast response speed, good selectivity, and simple sensor structure and showed potential application in therapeutic drug monitoring. Graphical Abstract
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-11
·
IF 5.3
Wang Chunxin,Deng Rong,Li Haiqin,Liu Zhigang,Niu Xiaofeng,Li Xiaochun
An intelligent colorimetric sensing platform integrated with in situ immunomagnetic separation function was developed for ultrasensitive detection of Escherichia coli O157: H7 ( E. coli O157: H7) in food. Captured antibody modified magnetic nanoparticles (cMNPs) and detection antibody/horseradish peroxidase (HRP) co-functionalized AuNPs (dHAuNPs) were firstly synthesized for targeted enrichment and colorimetric assay of E. coli O157: H7, in which remarkable signal amplification was realized by loading large amounts of HRP on the surface of AuNPs. Coupling with the optical collimation attachments and embedded magnetic separation module, a highly integrated optical device was constructed, by which in situ magnetic separation and high-quality imaging of 96-well microplates containing E. coli O157: H7 was achieved with a smartphone. The concentration of E. coli O157: H7 could be achieved in one-step by performing digital image colorimetric analysis of the obtained image with a custom-designed app. This biosensor possesses high sensitivity (1.63 CFU/mL), short detecting time (3 h), and good anti-interference performance even in real-sample testing. Overall, the developed method is expected to be a novel field detection platform for foodborne pathogens in water and food as well as for the diagnosis of infections due to its portability, ease of operation, and high feasibility. Graphical Abstract
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