JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Ying Jiang,Hao Lu,Lingli Lei,Daniel Scherman,Yingshuai Liu
The design of nanomedicines targeting specific tumor microenvironment (TME) characteristics, such as low pH, hypoxia, and elevated glutathione (GSH) concentration, holds significant potential for selectively killing cancer cells. In this study, we have developed a TME-responsive nanoparticle, i.e. , MIL-53(Fe)@MnO 2 stabilized with polyethylene glycol (FMP), which is capable of depleting GSH and sustainably generating hydroxyl radical (•OH) for enhanced chemodynamic therapy of cancer. After endocytosis, the FMP nanoparticles undergo gradual decomposition, resulting in GSH depletion while releasing Fenton-active Fe 2+ and Mn 2+ ions. These ions then subsequently catalyze a Fenton-like reaction to generate highly toxic •OH, ultimately inducing oxidative cell death. As a synergistic effect, the depletion of GSH further enhances therapeutic efficacy by inhibiting intracellular •OH scavenging. In vivo experiments demonstrated that this synergistically chemotherapeutic approach efficiently suppressed tumor growth without inducing significant systemic toxicity. This work presents a promising nanotherapeutic strategy that simultaneously depletes intracellular GSH and sustains cytotoxic •OH generation for enhanced tumor treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Peng Dong,Yun-Bo Chi,Deng-Ke Teng,Yuan-Qiang Lin,Ling-Yu Zhu,He-Qun Li,Jia-Yu Yang,Jia-Rui Du,Zong-Tao Zhang,Hai-Tao Ran,Guo-Qing Sui,Hui Wang,Qi-Meihui Wang
Ferroptosis, an iron-dependent, nonapoptotic form of regulated cell death, has become a new approach for antitumor treatment. However, the insufficient accumulation and poor penetration of ferroptosis inducers deep in tumors greatly limit their therapeutic effects. In this study, we constructed a cascade penetrating metal‒polyphenol ultrasonic molecular probe, Fe 3+ Cur-PFP@IR780-LIP (FCIPL). The nanoparticles (NPs) can penetrate deep into tumors in a stepwise fashion via specific targeting combined with cavitation effects resulting from acoustic droplet vaporization (ADV) and ultrasound-targeted microbubble destruction (UTMD) technology. Then, the drug can be successfully delivered into the mitochondria of tumor cells under the cascade response of ultrasound and the tumor microenvironment, inducing the ferroptosis domino cascade. The nanoparticles disassemble, releasing the Fenton reaction catalyst Fe 2+ and the ferroptosis inducer Cur, which together constitute the ferroptosis "amplifier," promoting the domino-like burst of lipid peroxide (LPO) and triggering ferroptosis. Simultaneously, the sonosensitizer IR780 is activated to induce sonodynamic therapy (SDT). Under the synergistic action of ferroptosis and SDT, a waterfall-like therapeutic effect is produced. In terms of diagnosis, this nanoplatform combines multimodal (ultrasound, photoacoustic, magnetic resonance and fluorescence) imaging to improve the diagnostic performance of anaplastic thyroid cancer (ATC) and provide a visualization strategy for early diagnosis. In this study, the advantages of ultrasound technology are exploited to achieve deep penetration of drugs and overcome the limitations of a single treatment modality, achieve optimal diagnostic and treatment effects, and provide new ideas for integrating ATC diagnosis and treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
·
2025
·
IF 1.7
Zhang Xiaojun,Wang Fang,Dai Yuna,Gao Zhaoyu,He Jianchao
Breast cancer (BC) refers to a malignant neoplasm that takes place in the epithelial tissue of the breast. α-Cyperone (α-CYP) is one of the principal active components of Cyperus rotundus . However, research on the role of α-CYP in the development of BC is still lacking. This study investigates the effect and underlying mechanism of α-CYP in the progression of BC. Our findings revealed that both low-dose and high-dose α-CYP inhibited the colony formation ability of MCF-7 and BT474 cells, accompanied by the decrease in Ki67 expression and the obstruction of the cell cycle. Moreover, α-CYP treatment increased the activity of caspase-3, which leads to an increase in apoptosis. Moreover, the combination of α-CYP and cisplatin (DDP) remarkably suppressed cell viability and further facilitated apoptosis, indicating that α-CYP could enhance the sensitivity of chemotherapeutic agents in BC cells. Further, α-CYP treatment decreased TRIM24 expression through the ubiquitin–proteasome pathway. Notably, α-CYP counteracted the robust proliferation of BC cells triggered by TRIM24 overexpression. Taken together, this study confirmed that α-CYP is an effective anticancer component for BC treatment. α-CYP inhibits proliferation and induces apoptosis of BC cells via the modulation of TRIM24.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2025
·
IF 5.6
Dapeng Lu,Lin Ma,Qingyue Zhang,Chengzhong Xing,Yanke Li
5-Fluorouracil (5-FU) is an effective drug in the treatment of colorectal cancer (CRC); however, the development of acquired resistance to 5-FU poses a great challenge in clinical practice. Through the analysis of public datasets, we found that reduced 3-Hydroxybutyrate Dehydrogenase 2 (BDH2) expression in CRC tissues predicted poor survival in CRC patients. Low expression of BDH2 in clinical CRC tissue samples predicted a higher degree of malignancy. In addition, public dataset analysis showed that GLI Pathogenesis Related 1 (GLIPR1) expression was elevated in CRC patients following 5-FU treatment, contrasting with the expression pattern of BDH2. We reported that in 5-FU-resistant CRC cell lines (LOVO/5-FU and HCT15/5-FU), BDH2 was downregulated, while the expression of GLIPR1 was increased. To explore the regulatory relationship between BDH2 and GLIPR1 in 5-FU resistance, BDH2 and GLIPR1 overexpression plasmid vectors were constructed to transfect 5-FU resistant CRC cell lines. BDH2 led to enhanced 5-FU sensitivity in 5-FU-resistant CRC cell lines and inhibited the malignant behavior of CRC-resistant cells in vitro . In nude mice with subcutaneous tumors and intraperitoneal injections of 5-FU, tumor tissues formed by BDH2-overexpressing cells exhibited slower growth and increased apoptosis. Mechanistically, the upregulation of BDH2 increases GLIPR1 promoter methylation, mediated by DNA methyltransferases, thereby inhibiting GLIPR1 expression. High expression of GLIPR1 reduces 5-FU sensitivity in 5-FU-resistant CRC cell lines, which abolished the impact of BDH2 expression. These results suggest that BDH2 inhibits GLIPR1 expression by increasing GLIPR1 promoter methylation, thereby enhancing 5-FU sensitivity of 5-FU-resistant CRC cell lines and inhibiting CRC progression.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Linna Chang,Yan Qin,Zhou Shen,Di Wu,Xiaoping Yin,Leyong Zeng
Reactive oxygen species (ROS) are critical in tumor therapy, but the antioxidant tumor microenvironment severely restricts the ROS-modulated antitumor efficacy. In this study, a photothermal-promoted cascade nanozyme (MnO 2 @Lap/PAH/L-Arg@Au) was constructed by modifying β-lapachone (β-Lap), poly(allylamine hydrochloride) (PAH), and L-arginine (L-Arg) and depositing gold nanoparticles (AuNPs) on hollow manganese dioxide (MnO 2 ), enhancing ROS-modulated catalytic/gas combination therapy. The pH- and glutathione-responsive hollow MnO 2 degradation released β-Lap to elevate the generation of hydrogen peroxide (H 2 O 2 ), which substantially activated and AuNPs-mediated hydroxyl radical (·OH) generation. Moreover, H 2 O 2 /·OH induces L-Arg to produce highly toxic nitric oxide (NO), and the Au nanoshell with high photothermal conversion efficiency (38.6 %) further enhanced ROS/NO generation. By reducing adenosine triphosphate production and inducing mitochondrial dysfunction, the combination of photothermal/catalytic/gas therapy greatly triggered cell apoptosis and inhibited tumor growth. Thus, the developed MnO 2 @Lap/PAH/L-Arg@Au nanozyme established a dynamic cascade pathway of “ROS-driven NO release”, and achieved photothermal-promoted catalytic/gas therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2025
·Vol. 15
·期 7
·458
·
IF 5.6
Shuai Zhao,Meimei Xu,Chenglong Lin,Weida Zhang,Dan Li,Yusi Peng,Masaki Tanemura,Yong Yang
Highly infectious and pathogenic viruses seriously threaten global public health, underscoring the need for rapid and accurate diagnostic methods to effectively manage and control outbreaks. In this study, we developed a comprehensive Surface-Enhanced Raman Scattering–Lateral Flow Immunoassay (SERS-LFIA) detection system that integrates SERS scanning imaging with artificial intelligence (AI)-based result discrimination. This system was based on an ultra-sensitive SERS-LFIA strip with SiO2-Au NSs as the immunoprobe (with a theoretical limit of detection (LOD) of 1.8 pg/mL). On this basis, a negative–positive discrimination method combining SERS scanning imaging with a deep learning model (ResNet-18) was developed to analyze probe distribution patterns near the T line. The proposed machine learning method significantly reduced the interference of abnormal signals and achieved reliable detection at concentrations as low as 2.5 pg/mL, which was close to the theoretical Raman LOD. The accuracy of the proposed ResNet-18 image recognition model was 100% for the training set and 94.52% for the testing set, respectively. In summary, the proposed SERS-LFIA detection system that integrates detection, scanning, imaging, and AI automated result determination can achieve the simplification of detection process, elimination of the need for specialized personnel, reduction in test time, and improvement of diagnostic reliability, which exhibits great clinical potential and offers a robust technical foundation for detecting other highly pathogenic viruses, providing a versatile and highly sensitive detection method adaptable for future pandemic prevention.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-12
·
IF 5.3
Xu Ke,Dong Hui,Wang Zuying,Chen Weitian,Zhou Yan,Cheng Menghao,Du Bingbing,Wei Xiuhua,Zhu Xu,Zhou Yanli,Li Zhaohui,Zhang Yintang,Xu Maotian
A molecular probe, 3-oxo-3H-phenoxazin-7-yl thiophene-2-carboxylate (RD), was synthesized using resorufin (Re) as the substrate for the specific recognition of glutathione (GSH). Ultrasonic waves were employed to enhance the reaction efficiency between the molecular probe and GSH. The molecular probe was designed to exhibit specific recognition ability for GSH. In the absence of GSH, the probe’s fluorescence is quenched; in the presence of GSH, a specific reaction restores the fluorescence intensity, achieving “turn-on” fluorescence detection. The detection limit for GSH was 5.52 μM, with a linear range of 5 μM to 1.2 mM. Additionally, an electrochemical detection method was integrated. The interaction between GSH and the modified electrode surface induces a change in the electrochemical signal, which can be used for the quantitative determination of GSH. The detection limit for GSH using this method was 8 μM, with a linear range of 10 μM to 1.2 mM. By combining these two detection modes, the reliability and accuracy of glutathione detection were significantly improved. Furthermore, the practical application of this method in human serum samples demonstrated its good selectivity and anti-interference ability, providing a reliable dual-mode analysis platform for clinical GSH detection. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Tingting Bai,Yanjia Liu,Ling Jian,Zhien Liu,Longxin Xu,Zhongwen Liu,Daohong Wu
β-lactoglobulin (β-Lg) is the most allergenic allergen in milk and can affect the health of infants and young children seriously. Detecting β-Lg in food products is an urgent requirement. The coordination of Cu(II) with single-strand DNA as metalloenzyme was used to catalyze TMB (3, 3′, 5, 5′-tetramethylbenzidine)-H 2 O 2 reaction to generate electrochemistry signal. Firstly, DNA 1 self-assembly on the electrode and hybridized with DNA 2 to form DNA 1 /DNA 2 double-stranded complex. Secondly, β-Lg combined with DNA 2 (aptamer) which partially complementary with DNA 1 via target-ligand interaction, β-Lg/DNA 2 complex formed and released, DNA-Cu(II)-AuNPs nanocomposites as catalysis for TMB-H 2 O 2 reaction was introduced subsequently. Thus, AuNPs coated with DNA-metalloenzyme as target-responsive and signal-amplification material for β-Lg detection was obtained. The detection limit of 1.69 pg/mL and linear range of 0.01—100 ng/mL was obtained. This aptasensor exhibits excellent selectivity for interfering ingredients, even if their concentration is 1000 times that of β-Lg. The recovery of standard addition experiment at three levels for four kinds of foods is in the range of 84.50–93.70 %, and RSD of 4.23–11.58 % was obtained. More importantly, β-Lg content detected in another six kinds of food products is consistent with β-Lg content reported which indicated good accuracy was obtained. In a word, this aptasensor exhibits prominent sensitivity, selectivity, accuracy, stability and repeatability which should be provided a universal substitution combination strategy for food allergens detection in future.
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Hongfei Yang,Liangni Qin,Jiaxu Xiao,Xiaoming Pan,Shiyun Han,Linwei Zhang,Jixiang Liang,Yu Si,Hao Wen,Long Li,Xingyu Wang,Xiaojuan Zhang,Dapeng Peng
Dexamethasone, a long-acting glucocorticoid, exhibits considerable structural similarity to other glucocorticoids. To avoid false-positive results in immunoassays due to cross-reactivity of antibodies with structural analogues, the preparation of highly specific antibodies is crucial. In this study, a novel hapten DEX-GA was designed and synthesized, and the monoclonal antibody (mAb) 3D1 was successfully prepared based on the hapten. The mAb 3D1 has the 50 % inhibitory concentration of 0.38 ng/mL for dexamethasone, with cross-reactivity below 7.45 % against twelve glucocorticoids including betamethasone, prednisone, triamcinolone and beclomethasone. Based on this antibody, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatography assay (GICA) were developed for accurate detection of dexamethasone in milk and animal tissues. The limits of detection of ic-ELISA in milk and animal tissues ranged from 0.132 to 0.215 μg/kg, which was a 1-3-fold increase in sensitivity compared with the previously reported ic-ELISA method. The GICA method established in this study had a visual limit of detection of 0.3 μg/L in milk. Notably, this constitutes the first application of GICA in animal tissues, demonstrating a visual detection limit of 0.2 μg/kg. The accuracy and reliability of both ic-ELISA and GICA were validated using liquid chromatography-tandem mass spectrometry.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2025
·
IF 4.5
Ying Yin,Xiang Cheng,Duoyang Fan,Yanpeng Fang,Haohan Li,Xingru Zhou,Hongqi Guo,Wenbin Zeng,Fei Chen
Photodynamic therapy (PDT), a noninvasive and spatiotemporally controlled cancer treatment, often suffers from the mitochondrial or lysosomal accumulation of conventional photosensitizers (PSs), primarily triggering apoptosis and limiting efficacy against apoptosis-resistant tumors. Targeting the plasma membrane offers a promising, yet less explored, strategy to induce immunogenic cell death pathways like ferroptosis and pyroptosis, which can overcome therapeutic resistance. However, PSs capable of simultaneously targeting the plasma membrane and activating both ferroptosis and pyroptosis are scarce. Here, we present a simple and rational π-bridge engineering strategy to develop multifunctional membrane-anchored PSs. Among our synthesized compounds, TDTP exhibits strong near-infrared (NIR) fluorescence, distinct aggregation-induced emission (AIE) characteristics, superior reactive oxygen species (ROS) generation, and precise plasma membrane localization. Upon light activation, TDTP induces synergistic ferroptotic-pyroptotic cell death, leading to highly efficient tumor ablation at nanomolar concentrations (IC50 = 26.07 nM). In vivo studies demonstrate that TDTP enables high-contrast NIR imaging and substantially inhibits tumor growth under image-guided PDT. This work highlights a facile yet powerful strategy for constructing membrane-specific PSs and introduces a rare class of theranostic agents capable of inducing dual cell death pathways.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2025
·Vol. 113
·期 9
·e35646
·
IF 3.4
Huiwen Wang,Yuting Wu,Mingrui Dai,Tingting Zhu,Daming Wu,Diya Leng
To investigate the antibacterial effect, mechanism, and cytotoxicity of Prussian blue/Cerium dioxide (PB/CeO 2 ) nanoparticles against Enterococcus faecalis (E. faecalis) and biofilm. PB/CeO 2 nanoparticles were synthesized and characterized. The antibacterial mechanism of nanoparticles was explored through peroxidase (POD) activity assay, hydroxyl radicals (·OH) detection, and measurement of bacterial reactive oxygen species (ROS) and glutathione (GSH)/glutathione disulfide (GSSG) levels. The biocompatibility of PB/CeO 2 was evaluated by Cell Counting Kit-8 (CCK-8) assay and histological examination of the major visceral organs of rats. The antibacterial effect of PB/CeO 2 was assessed using the colony-forming unit (CFU) method. The impact of PB/CeO 2 on E. faecalis biofilm on dentin slices was further observed with CLSM and SEM. ANOVA and t -test were applied for statistical analysis ( p < 0.05). PB/CeO 2 demonstrated significant antibacterial activity against E. faecalis , mainly when used with H 2 O 2 , significantly enhancing its antibacterial effect and effectively disrupting E. faecalis biofilms on dentin slices. PB/CeO 2 nanoparticles catalyzed ROS production, disrupting the antioxidant defense system of E. faecalis cells, damaging bacterial cell membranes, and ultimately causing bacterial death. PB/CeO 2 nanoparticles exhibit good biocompatibility at appropriate concentrations in vivo and in vitro. The novel multifunctional nanocomposite shows great antibacterial effects against E. faecalis and its biofilm, with low cytotoxicity and good biocompatibility, offering a novel disinfection strategy for root canal treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 10
·1-10
·
IF 5.3
Dong Huahua,Qv Kaige,Zhu Qunyan,Li Jinshuo,Liu Chang,Zhang Lin,Yang Wensheng
A closed bipolar electrode-based electrochemiluminescence (cBPE-ECL) sensor was developed for wireless uric acid (UA) detection. In the cBPE-ECL sensor design, a cobalt-based metal–organic framework (Co-MOF)-derived Au/CoO-modified carbon cloth (Au/CoO-C/CC) electrode served as a polarized anode for UA oxidation, where the generated faradaic current triggered the cathodic ECL of [Ru(bpy) 3 ] 2+ -[S 2 O 8 ] 2− at the polarized cathode electrode, enabling both quantitative and visual UA detection. The polarized anode was fabricated by incorporating Au nanoparticles into a Co-MOF structure on carbon cloth (CC), yielding an open architecture with enhanced charge transfer capabilities that significantly improved electrocatalytic activity toward UA. The as-prepared cBPE-ECL sensor exhibited excellent analytical performance, featuring a wide linear range (10–1000 µM), low detection limit (6 µM), high stability, good reproducibility, and strong anti-interference capabilities. The practical utility of this cBPE-ECL sensing platform was validated through successful UA detection in fetal bovine serum and artificial serum samples, achieving recoveries between 96.4 and 103.4%, demonstrating its potential for UA detection in clinical and biomedical applications. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）